Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0005238

Physical Location & Seq

Genomic Coordinates Forward (+)

69597972 .. 69605685

Gene Structure 7714 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG481300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5274 bp

ATGGCTGCCCCCAATAAGTTTTTTTCTCAGTTTATTTCCATGGTGAAATCATGGATCCCTTGGCGATCTGAGCCGGCTAATGTTTCGAGGGATTTTTGGATGCCTGATGATAGCTGTAGGGTATGTTATGAGTGTGATGCACAGTTCACTGTATTTAACCGTAAACATCATTGTAGACTTTGTGGACGAGTTTTCTGTGCCAAGTGTACAGAAAACTCAATTCCTGCCCCCTCTGGTGACTCAAGGATAGATCGGGAAGAGAGGGAAAGGATTCGCGTCTGCAATTTTTGTTACAAGCAACAACAGCAAGGCATAGCTGCTACTCCTGATAATGGAACACAGATTGCTAATCTAGATCTTAGTACCTCACCATCAGAAACAAGTTTTACTAGCTTTAAGTCATGCGGCACTGGTAACAGCAGTAGCTTTACTCTTAACTCAATCCCGTACTCAACTGGGCCATACCAACGACTTCAAAACAGTTCTGGTCTTAGCCCCTGTCAATCATCTCCCATGAAAACAAGCTCAGAAAAGCACAGCAAATATGCCTCCTGGAGGACCAATGATTTTGTTGCAGATATAGGGGATTCATCTCCAAATCATTATGAGATTTCAACAACCAGCATCAGGAGTGATGACGACGATGTTGACTATGGGACATATCAGTCAGATTCTAAGAATTATTCTCAAGTCAATGATTACTATGGTCATGTTGAGTTTTATGAGATGAGCAATCATGATGAATCACATAAAGTGCAACTTGATGGTGAAAACATTGATGCAAAGCATTTAAGCAGCTCCCCGTTGCTTCACAGTTTTAACTCGCAGGGTTCGGATCCACCACTTGAAAAGAAAGAAGATGAACATGATATGGGTGATGAATGTGCTTCCTCTTTATGTTCTGCAGGGGATGTTGACGTAGAACCTGTGGATTTTGAGAACAATGCCCTTCTTTGGCTTCCCCCTGAACCAGAAGATGAAGAAGATGAAAGGGAAACTGTTCTTCTTGATGATGACGATGATGGTGATGCTGCAGGAGAGTGGGGAACCTTACGCACATCAAGCAGTTTTGGCAGCGGGGAGTCTCGCAATAGGGATCGATCAGGTGAGGAGCATAAGAAGGTGATGAAGAATGTGGTTGATGGGCACTTTAGGGCTTTGGTAGCTCAGCTATTGCAGGTTGAGAACCTCCCTGTAGGCCAGGAAGGTGAAAATGAAAGTTGGTTGGAGATCATTACGTATCTGTCTTGGGAGGCTGCTACACTGTTAAAGCCAGATATGAGCAAAGGTGGAGGGATGGATCCTGGTGGTTATGTAAAAGAGATGGACCATTTAAAGATGGCAGTAGCAAAGATTGAAGCGCACCACCCTGATGTCCTTCTGGTAGAGAAATCAGTTTCTCGATATGCTCAGGAATATCTTCTTGCAAAAGACATATCACTTGTTCTTAATATCAAGAGGCCACTTTTAGAGCGCATAGCTCGCTGCACAGGTGCTCATATAGTTCCATCTATCGATCATCTCTCTTCACAAAAGTTGGGCTACTGTGAGACATTTCATGTGGGGAGGTTTCTGGAAGATCTTGGTTCTGCTGGCCAGGGTGGGAAGAAACTGGTGAAGACATTGATGTATTTTGAAGGCTGCCCAAAACCATTGGGTTGTACTATTTTACTCAGAGGTGCCAATGGGGATGAGTTGAAGAAAGTGAAGCATGTGGTTCAGTATGGGGTTTTTGCAGCATATCACTTGGCTTTGGAGACATCCTTCCTTGCTGATGAAGGTGCCTCTCTGCCTGAACTCCCATTCCAGTCTCCAATTACTGTGGCACTTCCAGATAAACCATCGAGTATTGAGAGGTCCATCTCAACAGTACCTGGTTTTAGAATTGGTGCCAACGAAAAGTCTCTGGGAGCTCAACCTCAGAGTGAACCACGAAGAGCCAACAGTGTCCCGATTACAGATTTTGACTCAGCAATTAAGAGTAGACCACCATGTTTACTAACTGGTCGTAGCTCTCTACCTGTTCGTCCGACTTCAAGTTCCACAAATTCTACCGGTTTACACTCTGCCCCACCTGGGAACGGTGTTACTTTTCATATCGCTGATAATCAAAATGAAATGGGCCCCAAAGATTCTTGGGTGGTGGAAACTTCTGCAACCAAACCTGGCTCTGATAATATGTCTAATCATCTTACTGCAAATAGCATGGGATCTTCAGAGGCCCTGGGACAAGGTATCTTCTCCAATACCCAAAATGATCCAAGTGTAAATCAGCTAGGTAGTTCAGGCAATCCAATGCTGCATCAAGATGGTCAAACCCATGCTGCAGATGCAGGAACCATGAATGAAGAGTTCCCTCCGTCACCTGCTGACCATCAGAGCATTTTAGTTTCCTTGTCATCTCGATGCGTGTGGAAGGGGACTGTCTGTGAGAGGTCACATCTGTTCCGAATTAAATACTACGGAAGTTTTGATAAACCTTTAGGACGGTTTTTGCGAGACCATTTATTTGATCAGACCTATCAATGCCATTCTTGCGAGATGCCATCAGAGGCACATGTTCATTGTTATACTCACCGACAGGGTACACTCACTATATCTGTTAAGAGGCTGCCAGAAATTTTCTTACCAGGTGAAAGGGAAGGGAAGATCTGGATGTGGCACAGATGTTTGAGGTGTCCAAGGATCAGTGGATTTCCTCCAGCTACTCGGAGAATAGTGATGTCTGATGCTGCATGGGGTTTGTCATTTGGGAAGTTTTTGGAGCTCAGTTTTTCAAACCATGCGGCTGCAAGCAGGGTGGCGAGCTGTGGCCATTCCTTACATAGAGATTGTCTTCGTTTCTATGGATTTGGGAAAATGGTTGCTTGCTTTCGGTATGCATCGATTGATGTTCATTCTGTCTACCTTCCACCCTCCAAACTGGATTTCATTTCCGAGAAGCAGGATTGGATACAAAAAGAAACTAATGAGGTTGTTGCCCGAGCAGAGCTTCTTTTCTCTGAAGTACTTAATGCTCTTAGTCAAATTTCAGAGAAAAGATCTGGTTCAGGGTCAATTACTAGTGGCATTGTAACAGCTGAATCCAGACACCAAATAGTGGAACTGGAAGGAATGTTACAGAAGGAGAAGGTGGAATTTGAGGAGTTACTTCAGAAAACTTTGAAGAGAGAACCTAAGAAGGGCCAACCTGTTATCGACATTCTGGAGATCAATCGATTGCGGAGGCAGTTGTTTTTTCAATCGTATATGTGGGACCACCGCTTGGTTTATGCAGCCAGTTTAGATAACAACAGCTTCCAGGATAGTTTGAGCAGCTCAATTCCAGCTGAGGATAAACCCGTGGCTACTAATGAAAAGCTTACTGAGATGGATGTAGAAATAAAGCCAGGAAAAGGCTATAATAGTTGTGATTCCTATCTGGTGGATGCAATGCTTAGTGATGGCTTTGACCACGAGGGAGGCTTTACTAGCGCTGCTATCAATGCAGACATGGTTCATGGAGAGGACAGGAGTGATGATTTGAATAAGGAAAAAGGTCAAGCTAATCTCCCTACTAGCACAAGTGTCGGCGCTCAATCTGATCCTTTGACACCAAGGACAGCTCTTCGTAGGGTCCTATCTGATGGTCAGCATCCTCTCATGCTGAATTTGTCAGATACCCTTGACACTGCATGGACCGGCGAAAATCTCATTGGAGTTGCGATAGCAAAGGAGAACTCGTGTCCAGTTCCTGACGTGGCCATAGAAAATTCTTCAAATGCCACTCCGGCAGAGGGATTAAATTTAGACCATGCAGAAGCCCAAAATGGGACCAAAGTTGCCCATTATGTGTCACCTGCATTGTCGACCAAGGGGTCTGAGAATATGGAAGACACAGTTAGCTGGTTAAAAATGCCCTTTTTGAACTTCTACCGTTCACTAAATAAGAATTTTTTATCTGCTGCTCAGAAGTTCGATACACTTGGTGGGTACAATCCAGTCTATGTGTCATCCTTTCGGGAGTTGGAACTGGAAGGTGGTGCTAGGCTGCTCTTGCCTGTGGGTGAAAACGATACTGTTGTCCCTGTATATGATGATGAGCCCACAAGTCTTATAGCCTATGCCCTTGTTTCATCAGATTATCAGTTACAAACCAGTGATGAGGGGGAAAGAGCAAAAGACAGTGGGGATATGATGGCCACAGCATCTTTTACTGATTCAGTCATTATGCATCCAGATGATGACACTGTGTCTGAAACTCATAGAAGTCTTGGGTCTACTGAAGAGAGCATACTATCAATGTCTGGATCTCGTGGCTCCCTGGGTTTGGATCCACTTTTATATACAAAGGCTTTGCATGCAAGAGTGTCTTTTGGAGATGATGGTCCTCTTGGTAAGGTTAAGTACTCTGTCACATGTTATTATGCAAAGCGTTTTGAAGCCTTAAGGAAGTTGTGTTGTCCCTCTGAGCTTGATTTTGTAAGATCCCTAAGTCGTTGTAAAAAGTGGGGAGCCCAAGGAGGTAAGAGCAATGTCTTTTTTGCAAAAACCTTGGATGACCGGTTTATCGTCAAACAAGTCACCAAGACAGAGCTGGAATCTTTCATTAAATTTGCACCTGCATATTTCAAGTACCTCTCTGAGTCAATTAGCACAGGAAGTCCAACCTGCCTGGCAAAAATTCTGGGGATATATCAGGTTATGTCGAAGCATGTTAAGGGAGGCAAAGAAACAAAGATGGATGTTTTGATTATGGAGAATCTTCTGTTTGGAAGGACTGTAACACGACTCTATGATCTCAAAGGATCTTCACGGTCAAGGTATAATCCTGATTCTAGTGGGAGTAACAAGGTTCTTCTGGATCAGAACTTGATTGAAGCAATGCCAACATCTCCAATCTTCGTGGGAAACAAGGCAAAGCGGCTGTTGGAGAGAGCTGTCTGGAATGATACCGCTTTCCTTGCCTCAATTGATGTGATGGATTATTCATTACTGGTCGGGGTGGATGAAGAGAAGCATGAGCTAGTACTTGGGATCATTGATTTCATGAGGCAGTATACATGGGATAAGCACCTTGAAACGTGGGTTAAAGCTTCAGGAATCCTTGGTGGGCCAAAGAACGCATCTCCGACTGTAATTTCACCAAAGCAATACAAAAAAAGGTTCAGGAAGGCCATGACAACCTATTTTCTGATGGTTCCAGATCAGTGGTCTCCTCCATCTATTGTTCCAAGTACATCCCAGTCTGATATCGGTGAAGAGAATGCACACGGTGGTGCCTCTGTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009555 GO:0009987 GO:0010008 GO:0010118 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032501 GO:0032502 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1757

Amino Acids

194.35

Weight (kDa)

5.5

Isoelectric Point (pI)

47.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 102	7e-18	FYVE zinc finger
Cpn60_TCP1	PF00118	440 - 590	3.2e-19	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1500 - 1666	3.3e-33	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1665 - 1722	6.9e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14270 AT3G14270
fragaria_vesca	FvH4_6g35770 FvH4_6g35770 FvH4_6g35770
malus_domestica	MD09G1173300.v1.1 MD17G1154000.v1.1
prunus_persica	Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1
pyrus_communis	pycom09g09070 pycom17g14660
rosa_chinensis	RchiOBHm_Chr2g0147811
rosa_laevigata	RLG00000020280
rosa_multiflora	Rmu_sc0000308.1_g000046
rosa_roxburghii	Rroxscaffold_2G00099330
rosa_rugosa	Rorug02G0401800 Rorug02G0401800 Rorug02G0401800 Rorug02G0401900
rosa_samantha	Rh2AG459000 Rh2BG472300 Rh2CG446400 Rh2DG481300
rosa_wichuraiana	Rw2G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	2374, 3847, 4609
AasI	GACNNNNNNGTC	2 cut(s)	664, 3856
Acc36I	ACCTGC	5 cut(s)	1168, 2374, 3847, 4609, 4658
AccB1I	GGYRCC	4 cut(s)	1679, 1781, 1889, 5258
AccB7I	CCANNNNNTGG	2 cut(s)	3100, 3265
AccI	GTMKAC	6 cut(s)	175, 1984, 2904, 3848, 4259, 5039
AccII	CGCG	1 cut(s)	276
AciI	CCGC	7 cut(s)	405, 1077, 2786, 3223, 3262, 4903, 4935
AcoI	YGGCCR	4 cut(s)	1594, 2812, 3741, 4179
AcuI	CTGAAG	5 cut(s)	2199, 3024, 3137, 4284, 5061
AdeI	CACNNNGTG	2 cut(s)	3968, 5255
AfeI	AGCGCT	1 cut(s)	3475
AflII	CTTAAG	1 cut(s)	4426
AflIII	ACRYGT	2 cut(s)	2557, 4397
AgeI	ACCGGT	2 cut(s)	2054, 4542
AhdI	GACNNNNNGTC	1 cut(s)	4231
AhlI	ACTAGT	1 cut(s)	3062
AjiI	CACGTC	1 cut(s)	3739
AjuI	GAANNNNNNNTTGG	4 cut(s)	2912, 2944, 3678, 3710
AleI	CACNNNNGTG	1 cut(s)	5256
AloI	GAACNNNNNNTCC	2 cut(s)	1788, 1820
Alw21I	GWGCWC	3 cut(s)	1498, 1915, 2769
Alw26I	GTCTC	7 cut(s)	1089, 1544, 1751, 1815, 1908, 2493, 5199
AlwNI	CAGNNNCTG	2 cut(s)	1874, 3734
Ama87I	CYCGRG	1 cut(s)	2982
Aor51HI	AGCGCT	1 cut(s)	3475
ApaI	GGGCCC	1 cut(s)	2126
ArsI	GACNNNNNNTTYG	2 cut(s)	2492, 2524
AsiGI	ACCGGT	2 cut(s)	2054, 4542
Asp700I	GAANNNNTTC	3 cut(s)	270, 999, 1419
AspLEI	GCGC	4 cut(s)	1363, 1476, 3476, 3575
AvaI	CYCGRG	1 cut(s)	2982
AvaII	GGWCC	8 cut(s)	558, 1327, 1857, 3256, 3616, 3678, 3813, 4367
BaeGI	GKGCMC	2 cut(s)	1149, 2126
BaeI	ACNNNNGTAYC	2 cut(s)	4047, 4080
BalI	TGGCCA	4 cut(s)	1596, 2814, 3743, 4181
BamHI	GGATCC	4 cut(s)	54, 835, 1300, 4312
BanI	GGYRCC	4 cut(s)	1679, 1781, 1889, 5258
BanII	GRGCYC	5 cut(s)	1915, 2126, 2769, 4086, 4498
BarI	GAAGNNNNNNTAC	4 cut(s)	3102, 3134, 4257, 4289
BauI	CACGAG	3 cut(s)	3455, 3721, 4293
BbsI	GAAGAC	3 cut(s)	1625, 2828, 3879
Bbv12I	GWGCWC	3 cut(s)	1498, 1915, 2769
BbvCI	CCTCAGC	1 cut(s)	3330
BcgI	CGANNNNNNTGC	2 cut(s)	3550, 3584
BciVI	GTATCC	1 cut(s)	2946
BclI	TGATCA	1 cut(s)	2512
BcoDI	GTCTC	7 cut(s)	1089, 1544, 1751, 1815, 1908, 2493, 5199
BcuI	ACTAGT	1 cut(s)	3062
BfaI	CTAG	9 cut(s)	353, 390, 2276, 3063, 3471, 3558, 4026, 4818, 5006
BfmI	CTRYAG	5 cut(s)	115, 903, 1032, 1194, 2325
BfoI	RGCGCY	2 cut(s)	3477, 3576
BfrI	CTTAAG	1 cut(s)	4426
BfuAI	ACCTGC	5 cut(s)	1168, 2374, 3847, 4609, 4658
BfuI	GTATCC	1 cut(s)	2946
BglI	GCCNNNNNGGC	1 cut(s)	3770
BglII	AGATCT	4 cut(s)	355, 1579, 2649, 3041
BlpI	GCTNAGC	1 cut(s)	1167
BmcAI	AGTACT	3 cut(s)	3009, 4388, 5010
Bme18I	GGWCC	8 cut(s)	558, 1327, 1857, 3256, 3616, 3678, 3813, 4367
BmeRI	GACNNNNNGTC	1 cut(s)	4231
BmeT110I	CYCGRG	1 cut(s)	2982
BmgBI	CACGTC	1 cut(s)	3739
BmrI	ACTGGG	2 cut(s)	465, 5218
BmuI	ACTGGG	2 cut(s)	465, 5218
BpiI	GAAGAC	3 cut(s)	1625, 2828, 3879
BpmI	CTGGAG	3 cut(s)	574, 2685, 3227
Bpu10I	CCTNAGC	2 cut(s)	1410, 3330
Bpu1102I	GCTNAGC	1 cut(s)	1167
BpuEI	CTTGAG	2 cut(s)	226, 672
Bsa29I	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
BsaAI	YACGTR	1 cut(s)	1239
BsaBI	GATNNNNATC	1 cut(s)	3417
BsaI	GGTCTC	2 cut(s)	2493, 5199
BsaWI	WCCGGW	2 cut(s)	2054, 4542
BsaXI	ACNNNNNCTCC	2 cut(s)	3707, 3737
Bse118I	RCCGGY	4 cut(s)	73, 2054, 3680, 4542
Bse3DI	GCAATG	3 cut(s)	3438, 4519, 4869
Bse8I	GATNNNNATC	1 cut(s)	3417
BseCI	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
BseJI	GATNNNNATC	1 cut(s)	3417
BseMI	GCAATG	3 cut(s)	3438, 4519, 4869
BseRI	GAGGAG	3 cut(s)	1124, 3158, 5187
BseSI	GKGCMC	2 cut(s)	1149, 2126
BsgI	GTGCAG	1 cut(s)	1471
Bsh1236I	CGCG	1 cut(s)	276
BshNI	GGYRCC	4 cut(s)	1679, 1781, 1889, 5258
BshTI	ACCGGT	2 cut(s)	2054, 4542
BshVI	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
BsiHKAI	GWGCWC	3 cut(s)	1498, 1915, 2769
BsiHKCI	CYCGRG	1 cut(s)	2982
BsiSI	CCGG	5 cut(s)	74, 2055, 3681, 3770, 4543
BslFI	GGGAC	8 cut(s)	670, 1934, 2241, 2434, 3269, 3826, 4049, 4428
BsmAI	GTCTC	7 cut(s)	1089, 1544, 1751, 1815, 1908, 2493, 5199
BsmFI	GGGAC	8 cut(s)	670, 1934, 2241, 2434, 3269, 3826, 4049, 4428
BsmI	GAATGC	1 cut(s)	5251
Bso31I	GGTCTC	2 cut(s)	2493, 5199
BsoBI	CYCGRG	1 cut(s)	2982
Bsp120I	GGGCCC	1 cut(s)	2122
Bsp1286I	GDGCHC	7 cut(s)	1149, 1498, 1915, 2126, 2769, 4086, 4498
Bsp1407I	TGTACA	1 cut(s)	206
Bsp1720I	GCTNAGC	1 cut(s)	1167
Bsp19I	CCATGG	1 cut(s)	39
BspACI	CCGC	7 cut(s)	405, 1077, 2786, 3223, 3262, 4903, 4935
BspDI	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
BspFNI	CGCG	1 cut(s)	276
BspHI	TCATGA	2 cut(s)	736, 5028
BspMAI	CTGCAG	3 cut(s)	907, 1036, 2329
BspMI	ACCTGC	5 cut(s)	1168, 2374, 3847, 4609, 4658
BspQI	GCTCTTC	2 cut(s)	1930, 3612
BspT107I	GGYRCC	4 cut(s)	1679, 1781, 1889, 5258
BspTI	CTTAAG	1 cut(s)	4426
BspTNI	GGTCTC	2 cut(s)	2493, 5199
BsrDI	GCAATG	3 cut(s)	3438, 4519, 4869
BsrFI	RCCGGY	4 cut(s)	73, 2054, 3680, 4542
BsrGI	TGTACA	1 cut(s)	206
BssAI	RCCGGY	4 cut(s)	73, 2054, 3680, 4542
BssNAI	GTATAC	1 cut(s)	5040
BssSI	CACGAG	3 cut(s)	3455, 3721, 4293
BssT1I	CCWWGG	8 cut(s)	39, 59, 2681, 3596, 3852, 4498, 4533, 5086
Bst1107I	GTATAC	1 cut(s)	5040
Bst2BI	CACGAG	3 cut(s)	3455, 3721, 4293
Bst6I	CTCTTC	9 cut(s)	252, 1531, 1930, 2343, 3161, 3612, 4260, 4986, 5235
BstAFI	CTTAAG	1 cut(s)	4426
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	1 cut(s)	1239
BstC8I	GCNNGC	7 cut(s)	75, 1483, 1594, 2794, 2806, 2869, 4341
BstDSI	CCRYGG	2 cut(s)	39, 3342
BstENI	CCTNNNNNAGG	1 cut(s)	553
BstFNI	CGCG	1 cut(s)	276
BstH2I	RGCGCY	2 cut(s)	3477, 3576
BstHHI	GCGC	4 cut(s)	1363, 1476, 3476, 3575
BstMAI	GTCTC	7 cut(s)	1089, 1544, 1751, 1815, 1908, 2493, 5199
BstNSI	RCATGY	5 cut(s)	1715, 2561, 4343, 4401, 4697
BstSFI	CTRYAG	5 cut(s)	115, 903, 1032, 1194, 2325
BstSLI	GKGCMC	2 cut(s)	1149, 2126
BstSNI	TACGTA	1 cut(s)	1239
BstUI	CGCG	1 cut(s)	276
BstV2I	GAAGAC	3 cut(s)	1625, 2828, 3879
BstXI	CCANNNNNNTGG	1 cut(s)	5190
BstZ17I	GTATAC	1 cut(s)	5040
Bsu15I	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
BsuI	GTATCC	1 cut(s)	2946
BsuTUI	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
BtgI	CCRYGG	2 cut(s)	39, 3342
BtrI	CACGTC	1 cut(s)	3739
BtsI	GCAGTG	1 cut(s)	3669
BveI	ACCTGC	5 cut(s)	1168, 2374, 3847, 4609, 4658
Cac8I	GCNNGC	7 cut(s)	75, 1483, 1594, 2794, 2806, 2869, 4341
CaiI	CAGNNNCTG	2 cut(s)	1874, 3734
CciI	TCATGA	2 cut(s)	736, 5028
CfoI	GCGC	4 cut(s)	1363, 1476, 3476, 3575
Cfr10I	RCCGGY	4 cut(s)	73, 2054, 3680, 4542
ClaI	ATCGAT	4 cut(s)	1099, 1515, 2885, 3217
CseI	GACGC	1 cut(s)	265
CsiI	ACCWGGT	2 cut(s)	1873, 2629
CspAI	ACCGGT	2 cut(s)	2054, 4542
CspCI	CAANNNNNGTGG	6 cut(s)	1803, 1838, 2781, 2816, 4866, 4901
DraI	TTTAAA	1 cut(s)	1335
DraIII	CACNNNGTG	2 cut(s)	3968, 5255
DrdI	GACNNNNNNGTC	2 cut(s)	664, 3856
DriI	GACNNNNNGTC	1 cut(s)	4231
DseDI	GACNNNNNNGTC	2 cut(s)	664, 3856
EaeI	YGGCCR	4 cut(s)	1594, 2812, 3741, 4179
Eam1104I	CTCTTC	9 cut(s)	252, 1531, 1930, 2343, 3161, 3612, 4260, 4986, 5235
Eam1105I	GACNNNNNGTC	1 cut(s)	4231
EarI	CTCTTC	9 cut(s)	252, 1531, 1930, 2343, 3161, 3612, 4260, 4986, 5235
Ecl136II	GAGCTC	2 cut(s)	1913, 2767
Eco105I	TACGTA	1 cut(s)	1239
Eco130I	CCWWGG	8 cut(s)	39, 59, 2681, 3596, 3852, 4498, 4533, 5086
Eco24I	GRGCYC	5 cut(s)	1915, 2126, 2769, 4086, 4498
Eco31I	GGTCTC	2 cut(s)	2493, 5199
Eco32I	GATATC	1 cut(s)	5233
Eco47I	GGWCC	8 cut(s)	558, 1327, 1857, 3256, 3616, 3678, 3813, 4367
Eco47III	AGCGCT	1 cut(s)	3475
Eco53kI	GAGCTC	2 cut(s)	1913, 2767
Eco57I	CTGAAG	5 cut(s)	2199, 3024, 3137, 4284, 5061
Eco88I	CYCGRG	1 cut(s)	2982
EcoICRI	GAGCTC	2 cut(s)	1913, 2767
EcoNI	CCTNNNNNAGG	1 cut(s)	553
EcoO109I	RGGNCCY	3 cut(s)	2123, 2221, 3616
EcoRV	GATATC	1 cut(s)	5233
EcoT14I	CCWWGG	8 cut(s)	39, 59, 2681, 3596, 3852, 4498, 4533, 5086
EcoT22I	ATGCAT	2 cut(s)	2884, 4215
EcoT38I	GRGCYC	5 cut(s)	1915, 2126, 2769, 4086, 4498
ErhI	CCWWGG	8 cut(s)	39, 59, 2681, 3596, 3852, 4498, 4533, 5086
FalI	AAGNNNNNCTT	4 cut(s)	744, 776, 4336, 4368
FaqI	GGGAC	8 cut(s)	670, 1934, 2241, 2434, 3269, 3826, 4049, 4428
FauI	CCCGC	1 cut(s)	1070
FbaI	TGATCA	1 cut(s)	2512
FblI	GTMKAC	6 cut(s)	175, 1984, 2904, 3848, 4259, 5039
FriOI	GRGCYC	5 cut(s)	1915, 2126, 2769, 4086, 4498
FspBI	CTAG	9 cut(s)	353, 390, 2276, 3063, 3471, 3558, 4026, 4818, 5006
GlaI	GCGC	4 cut(s)	1362, 1475, 3475, 3574
GsuI	CTGGAG	3 cut(s)	574, 2685, 3227
HaeII	RGCGCY	2 cut(s)	3477, 3576
HapII	CCGG	5 cut(s)	74, 2055, 3681, 3770, 4543
HgaI	GACGC	1 cut(s)	265
HhaI	GCGC	4 cut(s)	1363, 1476, 3476, 3575
Hin6I	GCGC	4 cut(s)	1361, 1474, 3474, 3573
HinP1I	GCGC	4 cut(s)	1361, 1474, 3474, 3573
HincII	GTYRAC	3 cut(s)	649, 916, 3849
HindII	GTYRAC	3 cut(s)	649, 916, 3849
HindIII	AAGCTT	2 cut(s)	3359, 5073
HpaII	CCGG	5 cut(s)	74, 2055, 3681, 3770, 4543
Hpy99I	CGWCG	1 cut(s)	644
HpyCH4IV	ACGT	4 cut(s)	918, 1238, 3738, 5063
HpySE526I	ACGT	4 cut(s)	918, 1238, 3738, 5063
HspAI	GCGC	4 cut(s)	1361, 1474, 3474, 3573
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
Ksp22I	TGATCA	1 cut(s)	2512
LguI	GCTCTTC	2 cut(s)	1930, 3612
LmnI	GCTCC	6 cut(s)	803, 1111, 1910, 2764, 4304, 4493
MabI	ACCWGGT	2 cut(s)	1873, 2629
MaeI	CTAG	9 cut(s)	353, 390, 2276, 3063, 3471, 3558, 4026, 4818, 5006
MaeII	ACGT	4 cut(s)	918, 1238, 3738, 5063
MfeI	CAATTG	1 cut(s)	4950
MhlI	GDGCHC	7 cut(s)	1149, 1498, 1915, 2126, 2769, 4086, 4498
MlsI	TGGCCA	4 cut(s)	1596, 2814, 3743, 4181
MluNI	TGGCCA	4 cut(s)	1596, 2814, 3743, 4181
MlyI	GAGTC	5 cut(s)	233, 1091, 1961, 4634, 4764
MmeI	TCCRAC	6 cut(s)	1206, 2054, 3987, 4670, 4890, 5135
Mox20I	TGGCCA	4 cut(s)	1596, 2814, 3743, 4181
Mph1103I	ATGCAT	2 cut(s)	2884, 4215
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	3 cut(s)	270, 999, 1419
MscI	TGGCCA	4 cut(s)	1596, 2814, 3743, 4181
MslI	CAYNNNNRTG	5 cut(s)	1371, 2307, 2404, 4218, 5256
Msp20I	TGGCCA	4 cut(s)	1596, 2814, 3743, 4181
MspA1I	CMGCKG	3 cut(s)	1077, 3080, 3329
MspCI	CTTAAG	1 cut(s)	4426
MspI	CCGG	5 cut(s)	74, 2055, 3681, 3770, 4543
MunI	CAATTG	1 cut(s)	4950
Mva1269I	GAATGC	1 cut(s)	5251
MvnI	CGCG	1 cut(s)	276
NaeI	GCCGGC	1 cut(s)	75
NcoI	CCATGG	1 cut(s)	39
NgoMIV	GCCGGC	1 cut(s)	73
NmuCI	GTSAC	6 cut(s)	236, 2361, 2436, 3834, 4393, 4561
NsiI	ATGCAT	2 cut(s)	2884, 4215
NspI	RCATGY	5 cut(s)	1715, 2561, 4343, 4401, 4697
OliI	CACNNNNGTG	1 cut(s)	5256
PaeI	GCATGC	1 cut(s)	4343
PagI	TCATGA	2 cut(s)	736, 5028
PaqCI	CACCTGC	3 cut(s)	2374, 3847, 4609
PasI	CCCWGGG	2 cut(s)	2224, 4303
PciI	ACATGT	2 cut(s)	2557, 4397
PciSI	GCTCTTC	2 cut(s)	1930, 3612
PcsI	WCGNNNNNNNCGW	1 cut(s)	2494
PctI	GAATGC	1 cut(s)	5251
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	3 cut(s)	270, 999, 1419
PflMI	CCANNNNNTGG	2 cut(s)	3100, 3265
PfoI	TCCNGGA	2 cut(s)	551, 3300
PinAI	ACCGGT	2 cut(s)	2054, 4542
PleI	GAGTC	5 cut(s)	233, 1090, 1961, 4633, 4764
PpsI	GAGTC	5 cut(s)	233, 1090, 1961, 4633, 4764
Ppu21I	YACGTR	1 cut(s)	1239
PpuMI	RGGWCCY	1 cut(s)	3616
PscI	ACATGT	2 cut(s)	2557, 4397
Psp124BI	GAGCTC	2 cut(s)	1915, 2769
Psp5II	RGGWCCY	1 cut(s)	3616
PspOMI	GGGCCC	1 cut(s)	2122
PspPPI	RGGWCCY	1 cut(s)	3616
PstI	CTGCAG	3 cut(s)	907, 1036, 2329
PstNI	CAGNNNCTG	2 cut(s)	1874, 3734
PvuII	CAGCTG	2 cut(s)	3080, 3329
RseI	CAYNNNNRTG	5 cut(s)	1371, 2307, 2404, 4218, 5256
SacI	GAGCTC	2 cut(s)	1915, 2769
SalI	GTCGAC	1 cut(s)	3847
SapI	GCTCTTC	2 cut(s)	1930, 3612
ScaI	AGTACT	3 cut(s)	3009, 4388, 5010
SchI	GAGTC	5 cut(s)	233, 1091, 1961, 4634, 4764
SduI	GDGCHC	7 cut(s)	1149, 1498, 1915, 2126, 2769, 4086, 4498
SexAI	ACCWGGT	2 cut(s)	1873, 2629
SfcI	CTRYAG	5 cut(s)	115, 903, 1032, 1194, 2325
SinI	GGWCC	8 cut(s)	558, 1327, 1857, 3256, 3616, 3678, 3813, 4367
SmiMI	CAYNNNNRTG	5 cut(s)	1371, 2307, 2404, 4218, 5256
SmlI	CTYRAG	3 cut(s)	241, 687, 4426
SmoI	CTYRAG	3 cut(s)	241, 687, 4426
SnaBI	TACGTA	1 cut(s)	1239
SpeI	ACTAGT	1 cut(s)	3062
SphI	GCATGC	1 cut(s)	4343
SsiI	CCGC	7 cut(s)	405, 1077, 2786, 3223, 3262, 4903, 4935
SspMI	CTAG	9 cut(s)	353, 390, 2276, 3063, 3471, 3558, 4026, 4818, 5006
SstI	GAGCTC	2 cut(s)	1915, 2769
StyI	CCWWGG	8 cut(s)	39, 59, 2681, 3596, 3852, 4498, 4533, 5086
TaiI	ACGT	4 cut(s)	921, 1241, 3741, 5066
TatI	WGTACW	6 cut(s)	206, 1661, 3007, 4386, 5008, 5216
TauI	GCSGC	3 cut(s)	408, 2789, 4906
TseFI	GTSAC	6 cut(s)	236, 2361, 2436, 3834, 4393, 4561
Tsp45I	GTSAC	6 cut(s)	236, 2361, 2436, 3834, 4393, 4561
TspGWI	ACGGA	2 cut(s)	2349, 2478
Van91I	CCANNNNNTGG	2 cut(s)	3100, 3265
Vha464I	CTTAAG	1 cut(s)	4426
VpaK11BI	GGWCC	8 cut(s)	558, 1327, 1857, 3256, 3616, 3678, 3813, 4367
XagI	CCTNNNNNAGG	1 cut(s)	553
XbaI	TCTAGA	1 cut(s)	352
XceI	RCATGY	5 cut(s)	1715, 2561, 4343, 4401, 4697
XcmI	CCANNNNNNNNNTGG	1 cut(s)	4573
XmiI	GTMKAC	6 cut(s)	175, 1984, 2904, 3848, 4259, 5039
XmnI	GAANNNNTTC	3 cut(s)	270, 999, 1419
XspI	CTAG	9 cut(s)	353, 390, 2276, 3063, 3471, 3558, 4026, 4818, 5006
ZrmI	AGTACT	3 cut(s)	3009, 4388, 5010
Zsp2I	ATGCAT	2 cut(s)	2884, 4215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.