Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0005238

Physical Location & Seq

Genomic Coordinates Reverse (-)

14677348 .. 14686482

Gene Structure 9135 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1173300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5496 bp

ATGGCTGCGCCCAACAAGATGTTCTCCCATTTCATTTCCATGGTGAAATCATGGATCCCTTGGCGATCAGAACCTGCTAATGTTTCGAGGGATTTTTGGATGCCTGATCGTAGCTGTAGGGTATGCTACGAGTGTGATGCACAGTTTACTGTATTTAACCGTAAACATCATTGTCGACTTTGTGGTCGAGTTTTCTGTGCTAAGTGTACAGAGAACTCAATTCCTGCCCCCTCTGGTGATCCAGGAACAGATCGGGAAGAGTGGGAGAAGATTCGTGTATGCAATTTTTGTTACAAGCAACGGGAACAAGGCGTAGCTATTCCTGATAGCGGAATCCTGATTGCCAACTTGGATCTTAGTTCTTCACTATCAGAAACAAGTTTTGCTAGCTTTAAGTCATGTGGCACTGGTAGCAGTAGTAGCTTCACTAACTCAATGCCACACTCAGCTGGACCATATCAACGACTTCAACTTGGTTCTGGCCTCAGCCCCAGTCAGTCATCTCTAATGGAAACTAACACAGAAAACCAGAGCAAATTCGGACCATGGAGGAACACTGATTTTGGTGCCAATATGTCTCCAAACCAATATGAAGTTGCAACTGCCAGGAGTGATGACGAGGATGTTGAGTCTGGTATTTTTCAGTCAGATTCAAAGAATTATTCTCAAGTCAGTGACTACTTTAGTCACATTGATTTTGATGAGATGAGCAACGATGATGGATCCCATAAGGTGCATCTTGATGGTGAAAATATTGATGCGAAAAATTTAAGTAGCTCCTCGTTACTTCCCAGTTATGACTCGCAGGTTTTGGAGGGAATCCCACAGCTCGAAAAAAAAGAAGATGAACATGATATTGGGGATGAATGTGAGGCATCTTCGTCTATGTATTCTGCAGGGGATGGTGATATACAACCTGTGGATTTTGAGAACAATGGACTTTTATGGCTCCCCCCCGAACCAGAAGATGAAGAAGATGAAAGGGAAACTGTTCTGCTTGATGATGACGATGATGGTGATGCTACAGGAGAGTGGGGCCATTTACGTGCATCAAGCAGTTTTGGAAGTGGGGAATATCGCAATAGGGATCGGTCAGGTGAGGAGCACAAGAAGGCCATGAAGAATGTAGTTGATGGACACTTTAGGGCTTTGGTAGCTCAACTATTGCAGGTTGAGAACCTTCCAATCGGCCAGGAAGGAGAAACAGAAGGTTGGTTGGAGATCATTACATCTCTGTCCTGGGAGGCTGCTACACTATTAAAGCCAGATATGAGCAAAGGTGGAGGGATGGACCCAGGTGGTTATGTAAAAGTAAAATGTATAGCTTCTGGATCTCGCTATGATAGTATGGTGGTCAAAGGAGTTGTGTGTAAGAAAAATGTGGCTCATCGACGAATGACATCCAAATTAGAGAAACCTCGGTTTATGATACTTGGTGGTGCTCTTGAGTACCAGCGTGTTTCTAACTCCTTGTCAAGTTTTGATACTCTATTGCAGCAGGAGATGGACCACTTAAAGATGGCAGTAGCAAAGATCGATGCACACCACCCTGATGTCCTTCTGGTAGAGAAATCAGTTTCTCGATATGCCCAAGAATATCTTCTTGCAAAAGACATATCACTTGTTCTGAATATCAAGAGGCCACTTTTAGAGCGCATAGCTCGCTGCACAGGTGCTCAAATAGTCCCTTCAATTGATCATCTCTCATCACAGAAGTTGGGCTACTGCGACTTATTTCATGTGGAGAGGTTCATGGAAGATCTTGGTTCTGCTGGTCAGGGTGGGAAAAAACTGGTGAAGACATTGATGTATTTTGAAGGCTGCCCAAAGCCATTGGGTTGTACTATTCTACTTAGAGGTGCTAATGGGGATGAGCTGAAAAAAGTGAAGCATGTGGTTCAGTATGGGATTTTTGCCGCATATCATTTGGCTTTGGAGACATCCTTCCTTGCCGATGAAGGAGCCTCTCTGCCAGAACTCCCATTGAACACTCCAATTACTGTGGCACTTCCAGATAAACCATCAAGCATTGAGAGGTCCATTTCCACGGTGCCTGGTTTTAGTGTTGCTGGCAATGGACAGTCTCGCAATGAACCACGAAGATCCAACAGCGTCCCAGTTTCAGATTTGGACTCAGCTATCAGAAGTATACAACCTCCTTTACTAAGTAGTCGTACCTCTCTACCCACTCCTCCCACTTCAGGTTTCACCATTTCTACATCTGTGTACCCCACTCCATCTGGGAATGCTTCAGATACCACATCTGTGTACTCTACTCCGTCTGGGAATGCTCCAGATACTTATCACAAGAGTCTTTCTTCTTATCATATGTTTGATAATCAAAATGAAATGAGTTCTAAAGAATTTTCAGAGGTGGAAAATTCTGCAACCAAAATTTGCTCTGATATCATGTCTAGTCATCTCGCTGGCAACAATTTGAGGTCTTTAGAGACTATGGGACAGGGTATCTTCTCAGTTGCCCAAAATGATGAAAGTGTAAGTACTGGAAATCAACTAGGTGGTTCAGACAATTCATTTCTGCATGAAGATGGTAAAACTCAAGCTGATGAACCAGGACCTATGAATGAAGAGTTTCCTCCGACACCTTCTGACCATCAGAGCATTTTGGTTTCCTTATCATCCCGGTGCGTGTGGAAGGGGACTGTCTGTGAGAGGTCGCATCTGTTTCGAATCAAATACTATGGAAGTTTCGATAAACCATTAGGACGGTTTTTACGAGACCATTTATTTGATCAGAGTTATCAATGCAATTCTTGCGAGATGCCTTCAGAAGCACATGTTCACTGTTATACTCATCGTCAGGGTACTCTCACTATATCTGTTAAGAGGCTGCCAGAAATTCTCTTACCAGGTGAAAAGGAAGGAAGGATCTGGATGTGGCACAGATGCTTGAAGTGTCCGAGGACAAATGGATTTCCTCCGGCTACTCGGAGAATAGTGATGTCTGATGCCGCATGGGGTTTGTCATTTGGGAAATTTTTGGAGCTCAGTTTTTCAAACCATGCGGCTGCAAGCAGAGTGGCGAGCTGCGGCCATTCCTTACATAGAGATTGTCTTCGTTTCTATGGATTTGGGAAGATGGTCGCTTGCTTTCGGTATGCATCAATTGATGTTTATTCTGTCTACCTTCCACCCGCCAAAGTGGATTTCAATTATGAGAAGCAGGAATGGATACAAAAAGAAACAGATGAGGTTATTGATCGGGCAGAGCTTCTTTTTTCTGAAGTACTTAATGTTCTTCGTCAAATTGCGGAGAAAAGATCTGGTTCAGGATCGCATAGTAGTGGCATGGTAACACCTGAATCGAGACACCAAATTGTCGAACTGGAAGGAATGTTACAAAGGGAGAAGGTGGAATTTGAGGAATTGCTCCAGAAAACTTTGAACAGAGAAGCTAAAAAGGGCCAGCCTGTTATTGACATTTTGGAGATCAATCGGCTGCGAAGGCAGCTGCTTTTCCAATCTTACATGTGGGACCATCGCTTAGTTTATGCTGCCAGTTTAGAGAATAACAGACACAGCGATGGTTTGAATAGCTCAATTCCAGATGAGGGGAAACCTGCAACTAATAGTGAAGATATTGCTGATAATGTGGCAATAAATCCAGGAAAAAGTTATAATAGTTGTGATTCCTTTCTTGTGGATACAATGCTTAACAAAGGATTTGACCACGGGGAAGACATTGCCAACACTGTCCACTCTGAAATGGTGAATAAAGAGAGAGACAGTGGCCGAAATTCAAAATATGAAAACGAAGATCAGTGTAATCTCTCTGATGGAGTTAGCACCTGTGATCAATCTGATCCTTTGAAACCAAGGGCAGGTATTCGTAAATCCCTCTCTGATGGTCAGTTTCCTGTCATAATGGATTTGTCAGATACCCTTGACACTGCATGGACTGGTGAAAATCAATGTGGATTTGGAACAGCGAAGGACAGTACCCGCACAGTTCCTGTTTTAGGTCTAGCAGATTCAAGTGCCTCTCCCGTGAAGGACGGATTGAACTTAGACCATGCAGAAGACCAGAATGGGCCCAAGCTTGCGCATTCTGCTTCTGGATTATCTACCAAGGGGTCTGAAAATATGGAAGACTCAGTAAGCTGGTTGAAAATGCCCTTTTTAAACTTCTACTTCAATAAGAATTTTTTATCTGCTTCTCAGAAGCTTGATACACTTGGTGAGTATAATCCGGTCTATGTTTCATCCTTTCGGGAGTTGGAACTTAAAGGTGGGGCTAGGCTGCTTCTGCCTGTGGGGGTAAATGATACTGTTGTCCCCGTATATGATGATGAGCCCACGAGTCTTATATCTTATGCCCTTGCCTCACCAGATTATCAATTGCAAATAAGTGACGATGGGGAAAGAACAAGAGACAATGGCGATGTTAGTTTGTCTGACTCTCTCGCTATGCAGTCACACCATCCTGATGATGACACAACATCTGAATCCCATCGAAGCTTTGGGTCTACTGAGGAGAGCCTTTTGCCCACATATGGATCTCGTAGCTCCCTGGGTTTGGATCCGCTTTCATACACAAAAGCTTTGCATGCAAGAGTTTCTTTCGGAGATGATAGCCCACTTGGTCAGGTTAAATACTCAGTGACGTGTTACTATGCAAAACGTTTTGAAGCCTTAAGAAGGATTTGCTGCCCATCTGAGCTTGACTATGTAAGATCTCTTAGTCGTTGTAAAAAGTGGGGAGCCCAAGGTGGAAAGAGCAATGTCTTTTTTGCTAAAAGCTTGGATGACCGGTTTATCATCAAACAAGTCACCAAGACGGAGTTGGAATCTTTCATAAAGTTTGCTCCCAGTTATTTCAAATACCTATCTGAATCAATTGGCACAAAAAGTCCAACATGCCTGGCAAAGATTTTGGGGATCTATCAGGTTACATCAAAACATCTTAAAGGAGGGAAAGAGTCGAAGATAGATGTCTTAGTTATGGAGAATCTTTTGTTTGGAAGAAATATTACGCGGCTCTATGATCTCAAAGGATCTTCACGGTCTCGTTATAATCCTGATTCTAGTGGGAGCAACAAAGTACTGCTGGATCAGAACTTGATTGAAGCAATGCCCACATCTCCGATCTTTGTGGGAAACAAGCCAAAGCGTGTGTTGGAGAGAGCTGTCTGGAATGATACTGCTTTTCTTGCTTCAATTGACGTAATGGATTATTCACTACTGGTTGGGGTGGACGAAGAGAATCATGAGTTAGTACTTGGAATTATTGATTTCATGAGGCAGTATACATGGGATAAGCACCTTGAAACTTGGGTTAAAGCTTCGGGCATCCTCGGTGGGCCAAAGAACGCTTCTCCAACTGTAGTTTCCCCAAAGCAGTACAAAAAAAGGTTCCGGAAAGCAATGACAACCTATTTTCTGATGGTTCCAGATCAGTGGTCTCCTCCGTCGATTGTTGCAAGTACATCCCAGTCTGATTTGGGCGAAGACACAGGTGGTAATTCGGTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000285 GO:0000322 GO:0000323 GO:0000324 GO:0000329 GO:0003674 GO:0003824 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005768 GO:0005773 GO:0005774 GO:0005942 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006810 GO:0006996 GO:0007033 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009555 GO:0009987 GO:0010008 GO:0010118 GO:0010256 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016301 GO:0016307 GO:0016310 GO:0016482 GO:0016740 GO:0016772 GO:0016773 GO:0019637 GO:0019898 GO:0030258 GO:0031090 GO:0031410 GO:0031982 GO:0032266 GO:0032501 GO:0032502 GO:0032991 GO:0035032 GO:0035091 GO:0042147 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061695 GO:0070772 GO:0071704 GO:0071840 GO:0090332 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0098852 GO:1901981 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00562 ko04070 ko04145 ko04810 map00562 map04070 map04145 map04810

Pfam Domains

Protein Families

Cpn60_TCP1 FYVE PIP5K

Protein Analysis

1832

Amino Acids

203.11

Weight (kDa)

5.41

Isoelectric Point (pI)

45.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	33 - 101	3.3e-18	FYVE zinc finger
Cpn60_TCP1	PF00118	407 - 651	4.4e-32	TCP-1/cpn60 chaperonin family
PIP5K	PF01504	1576 - 1742	1.4e-34	Phosphatidylinositol-4-phosphate 5-Kinase
PIP5K	PF01504	1742 - 1798	5.2e-08	Phosphatidylinositol-4-phosphate 5-Kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0005238)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14270 AT3G14270
fragaria_vesca	FvH4_6g35770 FvH4_6g35770 FvH4_6g35770
malus_domestica	MD09G1173300.v1.1 MD17G1154000.v1.1
prunus_persica	Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1 Prupe.3G031400_v2.0.a1
pyrus_communis	pycom09g09070 pycom17g14660
rosa_chinensis	RchiOBHm_Chr2g0147811
rosa_laevigata	RLG00000020280
rosa_multiflora	Rmu_sc0000308.1_g000046
rosa_roxburghii	Rroxscaffold_2G00099330
rosa_rugosa	Rorug02G0401800 Rorug02G0401800 Rorug02G0401800 Rorug02G0401900
rosa_samantha	Rh2AG459000 Rh2BG472300 Rh2CG446400 Rh2DG481300
rosa_wichuraiana	Rw2G037540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3639, 5034
AasI	GACNNNNNNGTC	1 cut(s)	183
Acc16I	TGCGCA	1 cut(s)	4065
Acc36I	ACCTGC	5 cut(s)	82, 796, 1159, 3589, 3833
AccB1I	GGYRCC	2 cut(s)	566, 2050
AccI	GTMKAC	5 cut(s)	175, 2149, 3144, 4487, 5267
AccII	CGCG	1 cut(s)	4996
AccIII	TCCGGA	1 cut(s)	5376
AclI	AACGTT	1 cut(s)	4642
AcoI	YGGCCR	3 cut(s)	1189, 3052, 3751
AcuI	CTGAAG	4 cut(s)	2184, 2235, 2772, 3264
AdeI	CACNNNGTG	1 cut(s)	4199
AfiI	CCNNNNNNNGG	8 cut(s)	329, 2201, 3572, 3842, 3980, 4514, 4537, 4659
AflII	CTTAAG	1 cut(s)	4654
AflIII	ACRYGT	3 cut(s)	2797, 3489, 4625
AgeI	ACCGGT	1 cut(s)	4770
AjiI	CACGTC	1 cut(s)	4626
AleI	CACNNNNGTG	1 cut(s)	2264
Alw21I	GWGCWC	4 cut(s)	1107, 1444, 1678, 3009
AlwNI	CAGNNNCTG	2 cut(s)	74, 3974
Aor13HI	TCCGGA	1 cut(s)	5376
ApaI	GGGCCC	1 cut(s)	4056
ArsI	GACNNNNNNTTYG	2 cut(s)	2732, 2764
AsiGI	ACCGGT	1 cut(s)	4770
Asp700I	GAANNNNTTC	3 cut(s)	990, 1599, 2592
AspLEI	GCGC	3 cut(s)	10, 1656, 4066
AsuC2I	CCSGG	1 cut(s)	2644
AsuII	TTCGAA	1 cut(s)	2689
AsuNHI	GCTAGC	1 cut(s)	386
AvaII	GGWCC	7 cut(s)	452, 542, 1291, 1507, 2037, 2576, 3496
BaeGI	GKGCMC	1 cut(s)	4056
BaeI	ACNNNNGTAYC	2 cut(s)	4278, 4311
BamHI	GGATCC	3 cut(s)	54, 722, 4540
BanI	GGYRCC	2 cut(s)	566, 2050
BanII	GRGCYC	4 cut(s)	3009, 4056, 4317, 4726
BarI	GAAGNNNNNNTAC	6 cut(s)	1706, 1738, 3342, 3374, 4975, 5007
BauI	CACGAG	1 cut(s)	4318
BbsI	GAAGAC	6 cut(s)	1805, 3068, 3705, 4047, 4116, 5475
Bbv12I	GWGCWC	4 cut(s)	1107, 1444, 1678, 3009
BbvCI	CCTCAGC	1 cut(s)	485
BcgI	CGANNNNNNTGC	4 cut(s)	119, 153, 2669, 2703
BciVI	GTATCC	2 cut(s)	3186, 3658
BclI	TGATCA	3 cut(s)	1696, 2752, 3814
BcnI	CCSGG	1 cut(s)	2644
BfaI	CTAG	6 cut(s)	387, 2415, 2516, 3986, 4257, 5046
BfmI	CTRYAG	4 cut(s)	115, 894, 1023, 5343
BfrI	CTTAAG	1 cut(s)	4654
BfuAI	ACCTGC	5 cut(s)	82, 796, 1159, 3589, 3833
BfuI	GTATCC	2 cut(s)	3186, 3658
BglII	AGATCT	3 cut(s)	1759, 3281, 4694
BmcAI	AGTACT	4 cut(s)	2503, 3249, 5064, 5238
Bme18I	GGWCC	7 cut(s)	452, 542, 1291, 1507, 2037, 2576, 3496
BmgBI	CACGTC	1 cut(s)	4626
BmrI	ACTGGG	5 cut(s)	486, 786, 2111, 4824, 5446
BmtI	GCTAGC	1 cut(s)	390
BmuI	ACTGGG	5 cut(s)	486, 786, 2111, 4824, 5446
BpiI	GAAGAC	6 cut(s)	1805, 3068, 3705, 4047, 4116, 5475
BpmI	CTGGAG	2 cut(s)	2277, 3377
Bpu10I	CCTNAGC	1 cut(s)	485
Bpu14I	TTCGAA	1 cut(s)	2689
BpuEI	CTTGAG	3 cut(s)	651, 1466, 2544
BpuMI	CCSGG	1 cut(s)	2644
Bsa29I	ATCGAT	1 cut(s)	1536
BsaAI	YACGTR	1 cut(s)	1046
BsaBI	GATNNNNATC	1 cut(s)	2301
BsaI	GGTCTC	3 cut(s)	2733, 5031, 5427
BsaWI	WCCGGW	3 cut(s)	4210, 4770, 5376
Bsc4I	CCNNNNNNNGG	8 cut(s)	329, 2201, 3572, 3842, 3980, 4514, 4537, 4659
Bse118I	RCCGGY	1 cut(s)	4770
Bse3DI	GCAATG	6 cut(s)	2080, 2095, 3702, 4747, 5097, 5391
Bse8I	GATNNNNATC	1 cut(s)	2301
BseAI	TCCGGA	1 cut(s)	5376
BseCI	ATCGAT	1 cut(s)	1536
BseJI	GATNNNNATC	1 cut(s)	2301
BseLI	CCNNNNNNNGG	8 cut(s)	329, 2201, 3572, 3842, 3980, 4514, 4537, 4659
BseMI	GCAATG	6 cut(s)	2080, 2095, 3702, 4747, 5097, 5391
BseRI	GAGGAG	5 cut(s)	769, 1115, 2181, 4508, 5415
BseSI	GKGCMC	1 cut(s)	4056
BsgI	GTGCAG	1 cut(s)	1651
Bsh1236I	CGCG	1 cut(s)	4996
BshNI	GGYRCC	2 cut(s)	566, 2050
BshTI	ACCGGT	1 cut(s)	4770
BshVI	ATCGAT	1 cut(s)	1536
BsiHKAI	GWGCWC	4 cut(s)	1107, 1444, 1678, 3009
BsiSI	CCGG	5 cut(s)	2644, 2942, 4211, 4771, 5377
BslFI	GGGAC	6 cut(s)	1670, 2099, 2472, 2674, 3509, 4280
BslI	CCNNNNNNNGG	8 cut(s)	329, 2201, 3572, 3842, 3980, 4514, 4537, 4659
BsmFI	GGGAC	6 cut(s)	1670, 2099, 2472, 2674, 3509, 4280
BsmI	GAATGC	3 cut(s)	2251, 2293, 4066
Bso31I	GGTCTC	3 cut(s)	2733, 5031, 5427
Bsp119I	TTCGAA	1 cut(s)	2689
Bsp120I	GGGCCC	1 cut(s)	4052
Bsp1286I	GDGCHC	7 cut(s)	1107, 1444, 1678, 3009, 4056, 4317, 4726
Bsp13I	TCCGGA	1 cut(s)	5376
Bsp1407I	TGTACA	1 cut(s)	206
Bsp19I	CCATGG	2 cut(s)	39, 545
BspDI	ATCGAT	1 cut(s)	1536
BspEI	TCCGGA	1 cut(s)	5376
BspFNI	CGCG	1 cut(s)	4996
BspHI	TCATGA	2 cut(s)	5227, 5256
BspMAI	CTGCAG	1 cut(s)	898
BspMI	ACCTGC	5 cut(s)	82, 796, 1159, 3589, 3833
BspOI	GCTAGC	1 cut(s)	390
BspT104I	TTCGAA	1 cut(s)	2689
BspT107I	GGYRCC	2 cut(s)	566, 2050
BspTI	CTTAAG	1 cut(s)	4654
BspTNI	GGTCTC	3 cut(s)	2733, 5031, 5427
BsrDI	GCAATG	6 cut(s)	2080, 2095, 3702, 4747, 5097, 5391
BsrFI	RCCGGY	1 cut(s)	4770
BsrGI	TGTACA	1 cut(s)	206
BssAI	RCCGGY	1 cut(s)	4770
BssNAI	GTATAC	2 cut(s)	2150, 5268
BssSI	CACGAG	1 cut(s)	4318
BssT1I	CCWWGG	6 cut(s)	39, 59, 545, 3836, 4089, 4726
Bst1107I	GTATAC	2 cut(s)	2150, 5268
Bst2BI	CACGAG	1 cut(s)	4318
Bst6I	CTCTTC	3 cut(s)	252, 2583, 5214
BstAFI	CTTAAG	1 cut(s)	4654
BstAPI	GCANNNNNTGC	1 cut(s)	2775
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	1 cut(s)	1046
BstBI	TTCGAA	1 cut(s)	2689
BstDSI	CCRYGG	4 cut(s)	39, 545, 2046, 3691
BstENI	CCTNNNNNAGG	2 cut(s)	3978, 4657
BstFNI	CGCG	1 cut(s)	4996
BstHHI	GCGC	3 cut(s)	10, 1656, 4066
BstNSI	RCATGY	5 cut(s)	1895, 2801, 3493, 4571, 4881
BstSFI	CTRYAG	4 cut(s)	115, 894, 1023, 5343
BstSLI	GKGCMC	1 cut(s)	4056
BstUI	CGCG	1 cut(s)	4996
BstV2I	GAAGAC	6 cut(s)	1805, 3068, 3705, 4047, 4116, 5475
BstXI	CCANNNNNNTGG	1 cut(s)	5418
BstZ17I	GTATAC	2 cut(s)	2150, 5268
Bsu15I	ATCGAT	1 cut(s)	1536
BsuI	GTATCC	2 cut(s)	3186, 3658
BsuTUI	ATCGAT	1 cut(s)	1536
BtgI	CCRYGG	4 cut(s)	39, 545, 2046, 3691
BtgZI	GCGATG	3 cut(s)	3485, 3558, 4416
BtrI	CACGTC	1 cut(s)	4626
BtsI	GCAGTG	1 cut(s)	3909
BveI	ACCTGC	5 cut(s)	82, 796, 1159, 3589, 3833
CaiI	CAGNNNCTG	2 cut(s)	74, 3974
CciI	TCATGA	2 cut(s)	5227, 5256
CfoI	GCGC	3 cut(s)	10, 1656, 4066
Cfr10I	RCCGGY	1 cut(s)	4770
ClaI	ATCGAT	1 cut(s)	1536
CseI	GACGC	1 cut(s)	2101
CsiI	ACCWGGT	1 cut(s)	2869
CspAI	ACCGGT	1 cut(s)	4770
CspCI	CAANNNNNGTGG	4 cut(s)	1983, 2018, 3021, 3056
DraI	TTTAAA	1 cut(s)	4143
DraIII	CACNNNGTG	1 cut(s)	4199
DrdI	GACNNNNNNGTC	1 cut(s)	183
DseDI	GACNNNNNNGTC	1 cut(s)	183
EaeI	YGGCCR	3 cut(s)	1189, 3052, 3751
Eam1104I	CTCTTC	3 cut(s)	252, 2583, 5214
EarI	CTCTTC	3 cut(s)	252, 2583, 5214
Ecl136II	GAGCTC	1 cut(s)	3007
Eco130I	CCWWGG	6 cut(s)	39, 59, 545, 3836, 4089, 4726
Eco24I	GRGCYC	4 cut(s)	3009, 4056, 4317, 4726
Eco31I	GGTCTC	3 cut(s)	2733, 5031, 5427
Eco32I	GATATC	1 cut(s)	2407
Eco47I	GGWCC	7 cut(s)	452, 542, 1291, 1507, 2037, 2576, 3496
Eco53kI	GAGCTC	1 cut(s)	3007
Eco57I	CTGAAG	4 cut(s)	2184, 2235, 2772, 3264
EcoICRI	GAGCTC	1 cut(s)	3007
EcoNI	CCTNNNNNAGG	2 cut(s)	3978, 4657
EcoO109I	RGGNCCY	1 cut(s)	2576
EcoRV	GATATC	1 cut(s)	2407
EcoT14I	CCWWGG	6 cut(s)	39, 59, 545, 3836, 4089, 4726
EcoT22I	ATGCAT	1 cut(s)	3124
EcoT38I	GRGCYC	4 cut(s)	3009, 4056, 4317, 4726
ErhI	CCWWGG	6 cut(s)	39, 59, 545, 3836, 4089, 4726
FalI	AAGNNNNNCTT	4 cut(s)	1584, 1616, 4564, 4596
FaqI	GGGAC	6 cut(s)	1670, 2099, 2472, 2674, 3509, 4280
FauI	CCCGC	2 cut(s)	3163, 3971
FauNDI	CATATG	2 cut(s)	2328, 4513
FbaI	TGATCA	3 cut(s)	1696, 2752, 3814
FblI	GTMKAC	5 cut(s)	175, 2149, 3144, 4487, 5267
FriOI	GRGCYC	4 cut(s)	3009, 4056, 4317, 4726
FspBI	CTAG	6 cut(s)	387, 2415, 2516, 3986, 4257, 5046
FspI	TGCGCA	1 cut(s)	4065
GlaI	GCGC	3 cut(s)	9, 1655, 4065
GsuI	CTGGAG	2 cut(s)	2277, 3377
HapII	CCGG	5 cut(s)	2644, 2942, 4211, 4771, 5377
HgaI	GACGC	1 cut(s)	2101
HhaI	GCGC	3 cut(s)	10, 1656, 4066
Hin6I	GCGC	3 cut(s)	8, 1654, 4064
HinP1I	GCGC	3 cut(s)	8, 1654, 4064
HincII	GTYRAC	1 cut(s)	176
HindII	GTYRAC	1 cut(s)	176
HindIII	AAGCTT	6 cut(s)	4058, 4184, 4477, 4560, 4759, 5301
HpaII	CCGG	5 cut(s)	2644, 2942, 4211, 4771, 5377
Hpy99I	CGWCG	2 cut(s)	1395, 5434
HpyCH4IV	ACGT	4 cut(s)	1045, 4625, 4642, 5184
HpySE526I	ACGT	4 cut(s)	1045, 4625, 4642, 5184
HspAI	GCGC	3 cut(s)	8, 1654, 4064
Kpn2I	TCCGGA	1 cut(s)	5376
Ksp22I	TGATCA	3 cut(s)	1696, 2752, 3814
MabI	ACCWGGT	1 cut(s)	2869
MaeI	CTAG	6 cut(s)	387, 2415, 2516, 3986, 4257, 5046
MaeII	ACGT	4 cut(s)	1045, 4625, 4642, 5184
MfeI	CAATTG	5 cut(s)	1692, 3126, 4358, 4857, 5178
MhlI	GDGCHC	7 cut(s)	1107, 1444, 1678, 3009, 4056, 4317, 4726
MlyI	GAGTC	8 cut(s)	638, 794, 2126, 2320, 4106, 4330, 4412, 4949
MmeI	TCCRAC	8 cut(s)	1197, 2130, 2624, 4218, 4785, 4898, 5118, 5363
Mph1103I	ATGCAT	1 cut(s)	3124
MroI	TCCGGA	1 cut(s)	5376
MroXI	GAANNNNTTC	3 cut(s)	990, 1599, 2592
MspA1I	CMGCKG	2 cut(s)	449, 3472
MspCI	CTTAAG	1 cut(s)	4654
MspI	CCGG	5 cut(s)	2644, 2942, 4211, 4771, 5377
MunI	CAATTG	5 cut(s)	1692, 3126, 4358, 4857, 5178
Mva1269I	GAATGC	3 cut(s)	2251, 2293, 4066
MvnI	CGCG	1 cut(s)	4996
NciI	CCSGG	1 cut(s)	2644
NcoI	CCATGG	2 cut(s)	39, 545
NdeI	CATATG	2 cut(s)	2328, 4513
NheI	GCTAGC	1 cut(s)	386
NmuCI	GTSAC	6 cut(s)	674, 686, 4370, 4434, 4621, 4789
NsbI	TGCGCA	1 cut(s)	4065
NsiI	ATGCAT	1 cut(s)	3124
NspI	RCATGY	5 cut(s)	1895, 2801, 3493, 4571, 4881
NspV	TTCGAA	1 cut(s)	2689
OliI	CACNNNNGTG	1 cut(s)	2264
PaeI	GCATGC	1 cut(s)	4571
PagI	TCATGA	2 cut(s)	5227, 5256
PasI	CCCWGGG	1 cut(s)	4531
PciI	ACATGT	2 cut(s)	2797, 3489
PcsI	WCGNNNNNNNCGW	1 cut(s)	2734
PctI	GAATGC	3 cut(s)	2251, 2293, 4066
PdmI	GAANNNNTTC	3 cut(s)	990, 1599, 2592
PfoI	TCCNGGA	2 cut(s)	241, 3625
PinAI	ACCGGT	1 cut(s)	4770
PleI	GAGTC	8 cut(s)	637, 794, 2126, 2319, 4106, 4329, 4412, 4948
PpsI	GAGTC	8 cut(s)	637, 794, 2126, 2319, 4106, 4329, 4412, 4948
Ppu21I	YACGTR	1 cut(s)	1046
PpuMI	RGGWCCY	1 cut(s)	2576
PscI	ACATGT	2 cut(s)	2797, 3489
PsiI	TTATAA	2 cut(s)	3639, 5034
Psp124BI	GAGCTC	1 cut(s)	3009
Psp1406I	AACGTT	1 cut(s)	4642
Psp5II	RGGWCCY	1 cut(s)	2576
PspOMI	GGGCCC	1 cut(s)	4052
PspPPI	RGGWCCY	1 cut(s)	2576
PsrI	GAACNNNNNNTAC	2 cut(s)	3240, 3272
PstI	CTGCAG	1 cut(s)	898
PstNI	CAGNNNCTG	2 cut(s)	74, 3974
PvuII	CAGCTG	2 cut(s)	449, 3472
SacI	GAGCTC	1 cut(s)	3009
SalI	GTCGAC	1 cut(s)	174
ScaI	AGTACT	4 cut(s)	2503, 3249, 5064, 5238
SchI	GAGTC	8 cut(s)	638, 794, 2126, 2320, 4106, 4330, 4412, 4949
SduI	GDGCHC	7 cut(s)	1107, 1444, 1678, 3009, 4056, 4317, 4726
SexAI	ACCWGGT	1 cut(s)	2869
SfcI	CTRYAG	4 cut(s)	115, 894, 1023, 5343
SfuI	TTCGAA	1 cut(s)	2689
SinI	GGWCC	7 cut(s)	452, 542, 1291, 1507, 2037, 2576, 3496
SmlI	CTYRAG	4 cut(s)	666, 1445, 2559, 4654
SmoI	CTYRAG	4 cut(s)	666, 1445, 2559, 4654
SphI	GCATGC	1 cut(s)	4571
SspI	AATATT	2 cut(s)	754, 4990
SspMI	CTAG	6 cut(s)	387, 2415, 2516, 3986, 4257, 5046
SstI	GAGCTC	1 cut(s)	3009
StyI	CCWWGG	6 cut(s)	39, 59, 545, 3836, 4089, 4726
TaiI	ACGT	4 cut(s)	1048, 4628, 4645, 5187
TaqII	GACCGA	1 cut(s)	1080
TatI	WGTACW	9 cut(s)	206, 1841, 2268, 2501, 3247, 5062, 5236, 5361, 5444
TauI	GCSGC	5 cut(s)	1919, 2975, 3029, 3054, 4999
TseFI	GTSAC	6 cut(s)	674, 686, 4370, 4434, 4621, 4789
Tsp45I	GTSAC	6 cut(s)	674, 686, 4370, 4434, 4621, 4789
TspGWI	ACGGA	4 cut(s)	2268, 4032, 4814, 5418
Vha464I	CTTAAG	1 cut(s)	4654
VpaK11BI	GGWCC	7 cut(s)	452, 542, 1291, 1507, 2037, 2576, 3496
XagI	CCTNNNNNAGG	2 cut(s)	3978, 4657
XceI	RCATGY	5 cut(s)	1895, 2801, 3493, 4571, 4881
XcmI	CCANNNNNNNNNTGG	2 cut(s)	4478, 4801
XmiI	GTMKAC	5 cut(s)	175, 2149, 3144, 4487, 5267
XmnI	GAANNNNTTC	3 cut(s)	990, 1599, 2592
XspI	CTAG	6 cut(s)	387, 2415, 2516, 3986, 4257, 5046
ZrmI	AGTACT	4 cut(s)	2503, 3249, 5064, 5238
Zsp2I	ATGCAT	1 cut(s)	3124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.