AT3G14570

Callose synthase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0016003
Physical Location & Seq
Forward (+)
4892410 .. 4902880
10471 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14570.1

Sequence Viewer

Length: 5931 bp
ATGTCTCACGAAATCGTCCCCGTTGATCCCATTGATGTTCCTAGCACGAGTTACTCACGGCCAATTCTGGGTCCTCGTGAAGATTCGCCGGAACGTGCTACCGAGTTCACGAGATCCCTAACTTTTCGGGAACATGTGTCGTCGGAGCCTTTTGATAGCGAGAGGCTTCCAGCGACATTAGCTTCCGAGATTCAGAGGTTTCTTCGTATTGCTAACTTGGTTGAGTCTGAGGAACCTCGAATTGCTTATCTATGCCGATTTCATGCTTTCGAGATAGCCCACCACATGGATAGAAACTCTACCGGAAGAGGTGTACGGCAGTTCAAAACTTCTCTTCTTCAAAGGCTTGAACTGGATGAAGAGTTCACTGTGAGAAGAAGAAAGGAGAAGAGTGATGTTCGGGAACTCAAACGTGTTTATCATGCTTACAAGGAATATATTATCAGACATGGTGCAGCTTTCAATTTGGATAACAGTCAGCGAGAGAAGTTGATTAACGCACGCAGAATTGCTTCTGTTTTGTATGAAGTTCTCAAGACAGTCACAAGTGGCGCTGGCCCGCAGGCCATTGCGGATAGAGAAAGTATTCGAGCAAAATCTGAATTTTATGTGCCGTATAACATTCTTCCTCTCGATAAAGGAGGGGTACACCAAGCAATTATGCATCTGCCTGAGATTAAAGCTGCTGTTGCAATTGTACGTAACACTCGCGGGTTGCCTCCTCCAGAGGAATTTCAGAGACACCAGCCTTTTCTGGATTTATTTGAATTTCTGCAGTATGCCTTTGGGTTCCAGAATGGAAATGTTGCCAATCAGAGAGAGCATCTGATTCTACTACTCAGTAACACCATTATACGGCAACCACAGAAGCAGTCTTCAGCTCCAAAGTCTGGTGATGAGGCAGTAGATGCATTGATGAAGAAGTTTTTTAAGAACTACACGAACTGGTGCAAATTTCTTGGGAGAAAAAATAACATTAGGTTGCCCTATGTGAAACAGGAAGCCCTACAATATAAAACATTGTATATAGGACTCTACCTTCTCATATGGGGAGAGGCGTCGAACTTGAGGTTCATGCCAGAGTGCCTGTGTTATATTTTCCACCACATGGCATATGAATTGCATGGAGTTTTAACCGGTGCAGTTAGTATGATAACTGGGGAGAAGGTTGCTCCAGCATATGGTGGAGGACACGAATCTTTCCTTGCGGATGTAGTTACTCCCATATACATGGTTGTCCAAAAGGAAGCTGAGAAGAATAAGAATGGAACTGCTGATCATTCTATGTGGAGAAACTACGATGATCTGAACGAGTTCTTCTGGTCTCTTGAATGCTTCGAAATAGGTTGGCCAATGCGTCCCGAGCACGATTTTTTCTGTGTAGAATCATCAGAAACCAGTAAACCAGGGAGATGGCGAGGAATGCTTAGATTTCGCAAGCAGACAAAGAAAACGGATGAAGAAATAGAGGATGACGAAGAACTTGGGGTTCTCAGTGAGGAACAACCTAAACCAACCTCAAGATGGCTGGGGAAGACAAATTTTGTAGAGACTCGTTCCTTTTGGCAGATATTCCGGAGCTTTGACAGAATGTGGAGTTTCTTTGTTTTGTCCCTACAGGCATTGATAATTATGGCTTGTCATGATGTGGGATCTCCACTCCAAGTGTTTAATGCTAATATATTCGAAGACGTCATGAGCATTTTCATTACCTCAGCAATTCTAAAGCTTATAAAAGGTATTCTTGACATAATCTTCAAGTGGAAAGCAAGAAACACAATGCCGATCAATGAAAAGAAGAAGCGGTTGGTGAAGTTGGGCTTTGCTGCGATGTGGACTATTATTCTACCAGTGCTGTATTCTCATTCAAGAAGGAAATACATTTGTTATTTCACAAATTACAAAACCTGGCTTGGTGAATGGTGTTTTTCTCCATACATGGTGGCAGTCACGATATACCTGACAGGAAGTGCAATTGAGTTGGTCTTGTTTTTCGTTCCTGCCATCAGCAAATACATTGAGACCTCCAATCATGGCATCTTTAAAACTTTGTCTTGGTGGGGACAGCCTAGGTTATATGTTGGGCGAGGAATGCAAGAAACTCAGGTGTCACAATTCAAATACACTTTCTTCTGGATTCTGGTTCTCCTGACCAAGTTTGCTTTCAGCTATGCATTTGAGATAAAGCCTCTAATAGAACCTACACGACTGATAATGAAAGTTGGTGTGCGGAATTATGAATGGCATGAAATTTTCCCAGAAGTGAAGAGTAATGCTGCTGCAATTGTTGCAGTATGGGCGCCAATAATGGTGGTCTATTTCATGGATACACAAATATGGTATTCAGTTTACTGTACGATCTTTGGTGGACTGTATGGTGTTCTGCACCACCTTGGTGAGATTCGAACTTTAGGCATGTTGAGAGGCCGGTTTCATACTTTACCTTCAGCTTTCAATGCATCCCTTATTCCACACTCAACAAAAGATGAGAAAAGGAGAAAACAAAGAGGTTTTTTCCCCTTCAATTTAGGCAGGGGGTCTGATGGCCAGAAAAATAGTATGGCCAAGTTTGTCTTGGTCTGGAACCAAGTCATTAACAGTTTCAGAACAGAAGATCTGATAAGTAACAAGGAATTAGATTTGATGACGATGCCGCTGTCATCGGAGGTATTATCAGGAATCATCCGTTGGCCCATTTTCCTCCTTGCAAATAAGTTTTCAACAGCTCTGAGCATTGCAAAAGATTTTGTCGGGAAGGATGAGGTTCTCTATCGAAGGATTCGGAAAGACGAGTACATGTACTACGCAGTAAAAGAGTGCTATGAATCTTTGAAGTACATTCTTCAGATTCTTGTTGTTGGTGATCTTGAGAAAAAGATTATATCTGGTATAATAAACGAGATAGAGGAAAGCATCAGACAGTCAAGTTTGCTTGAAGAATTTAAGATGGCTGAGCTCCCAGCGTTGCATGACAAATGTATTGAGCTGGTTCAACTCCTGGTTGAGGGAAGTGCCGAGCAACTCCAGGTTGAGAAAAGCGAAGAACTGCATGGTAAACTTGTAAAGGCTCTTCAGGATATCTTTGAGCTAGTAACAAATGATATGATGGTACATGGTGACAGAATTCTGGATTTGCTCCAGTCTCGGGAGGGGTCAGGAGAAGACACAGGTATTTTTATGAGGGTTATAGAACCACAGTTGTTTGAATCCTACGGAGAATGGAGATGCATTCATTTTCCGCTGCCAGATAGTGCTTCATTGAGTGAACAGATCCAGCGTTTTCTGTTATTGTTAACTGTCAAAGATAGTGCAATGGACATACCAGAGAACTTAGATGCTCGGCGGCGCCTATCTTTCTTTGCTACTTCGCTTTTCATGGATATGCCCGATGCTCCGAAAGTACGCAATATGATGTCATTCAGTGTTTTGACACCGCATTATCAGGAGGATATTAACTATTCAACGAACGAACTACATTCCACCAAGTCTAGTGTGTCAATCATCTTTTATATGCAGAAGATTTTTCCAGATGAGTGGAAAAACTTTCTTGAGCGAATGGGATGTGATAATTTAGACGCCTTAAAGAAAGAGGGTAAAGAAGAAGAGCTTCGAAACTGGGCATCATTTCGTGGACAAACATTGAGTAGAACAGTTCGTGGTATGATGTATTGCCGAGAAGCTCTGAAATTGCAAGCCTTCCTTGACATGGCAGATGACGAGGATATTCTTGAAGGATACAAGGACGTGGAACGAAGTAACAGACCTTTAGCCGCACAACTTGATGCATTGGCAGATATGAAATTCACGTATGTTGTTTCTTGCCAAATGTTTGGAGCCCAAAAATCATCTGGGGATCCTCACGCGCAGGATATACTTGACCTGATGATAAAGTATCCATCTCTCCGTGTTGCATACGTTGAGGAGAGGGAAGAAATCGTGTTGGATGTTCCAAAGAAGGTTTACTACTCTATATTGGTAAAAGCTGTTAACGGTTTCGATCAGGAAATTTACCGTGTCAAACTTCCTGGACCACCTAACATTGGAGAGGGAAAGCCTGAGAATCAAAATCACGCCATAGTTTTCACTCGAGGTGAAGCACTTCAGACGATCGACATGAACCAAGATCACTACCTGGAAGAGGCTTTCAAAATGAGAAATCTCCTTCAAGAATTTCTTCGAAACCGTGGAAGAAGACCTCCAACAATTCTTGGTTTAAGGGAACACATATTCACCGGAAGTGTTTCTTCTCTAGCATGGTTCATGTCATATCAAGAAACCAGTTTTGTCACAATCGGTCAAAGGCTTCTTGCTAATCCTCTCAGAGTCCGGTTTCACTATGGGCATCCAGATGTCTTCGACAGAATATTTCACATAACAAGGGGTGGCATCAGTAAATCATCGAGAACTATTAACTTAAGCGAGGACGTTTTCGCAGGTTATAATACTACCTTACGACGTGGATGCATAACCTACAATGAGTACCTCCAAGTTGGAAAAGGTCGTGATGTTGGCCTCAATCAAATCTCAAAGTTTGAGGCCAAGGTGGCTAACGGAAACAGTGAACAGACTATTAGCCGTGACATATACCGTTTAGGACAACGTTTTGATTTCTTCAGAATGTTGTCTTGTTATTTCACAACCATTGGATTCTACTTCAGTAGCTTGATTTCCGTGATCGGGATTTATATTTATCTCTATGGTCAACTCTACCTCGTACTCAGTGGGTTGCAGAAGACGCTTATTCTTGAGGCCAAGGTGAAAAACATAAAATCATTGGAAACAGCCCTTGCCTCTCAATCATTTATCCAGCTAGGTCTCTTGACCGGTCTACCAATGGTGATGGAGATTGGGTTGGAGAAAGGATTTCTTATAGCCTTTCAAGATTTCATACTCATGCAGCTACAGCTTGCTGCATTCTTCTTCACTTTCTCTCTAGGAACTAAAACGCATTACTTTGGCAGAACAATTCTCCATGGAGGTGCCAAATACAGACCCACAGGGCGTAAAGTAGTGGTGTTCCATGCCAACTTCAGTGAAAATTACAGATTATATTCTCGAAGCCATTTCATCAAAGGTTTTGAGCTCATGATTCTTCTGGTCGTCTACGAGTTGTTCAAGCATACATCTCAAAGCAACATGGCTTACTCATTCATCACTTTCTCAGTGTGGTTCATGTCTTTTACTTGGTTATGCGCCCCGTTTCTCTTCAACCCTTCAGGCTTTACCTGGGAAATAATAGTCGGTGACTGGAGAGACTGGAACAGGTGGATCAAAGAGCAAGGTGGAATAGGGATTCAGCAAGACAAGAGCTGGCAATCTTGGTGGAACGACGAGCAAGCCCATCTACGTGGCTCTGGTGTTGGAGCTCGCTGTTTGGAGATAATTTTGTCCCTCAGGTTCTTTGTGTATCAGTATGGTTTGGTATATCACCTTGACATTACCCAAAGCAACACAAATATCATAGTCTACGCGCTTTCTTGGGTCGTGATTCTCGCCACATTCTTCACAGTCAAGGCAGTAGACCTAGGAAGACAACTCTTCAGCACAAGAAAGCATCTCGTGTTTCGGTTCTTCAAAGTCTTCGTCTTCGTGAGCATACTTACAATCATAATCACTCTAGCAAACATCTGTCACCTCTCTGTCAAGGACCTTCTAGTCTCTTGCTTAGCTTTCTTGCCCACTGGTTGGGGTCTGATTCTGATTGCACAAGCGGTTAGGCCAAAGATAGAAGGAACGAGCTTATGGGAGTTCACACAGGTTCTTGCTCGAGCTTATGATTATGGAATGGGCGTTGTTCTGTTTGCACCAATGGCTATCTTAGCATGGCTTCCTATTATTTCTGCGTTTCAGACTCGTTTTCTATTCAACGAAGCTTTTAACAGAAGACTTCAAATTCAACCTATTCTTGCAGGGAAGAAGAAGAATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1976

Amino Acids

228.48

Weight (kDa)

8.73

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 64 - 183 3.6e-08 Vta1 like
FKS1_dom1 PF14288 339 - 450 3.9e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 898 - 1100 5e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1107 - 1873 1.6e-213 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1733, 4440
AasI GACNNNNNNGTC 1 cut(s) 5654
AatII GACGTC 1 cut(s) 1695
Acc36I ACCTGC 1 cut(s) 4424
AccB1I GGYRCC 3 cut(s) 2299, 3345, 4979
AccB7I CCANNNNNTGG 5 cut(s) 286, 890, 1108, 1181, 5685
AccI GTMKAC 4 cut(s) 4828, 5103, 5466, 5520
AccII CGCG 3 cut(s) 711, 3863, 5471
AccIII TCCGGA 1 cut(s) 1575
AclI AACGTT 1 cut(s) 4600
AclWI GGATC 7 cut(s) 20, 108, 1660, 3265, 3848, 3861, 5276
AcoI YGGCCR 4 cut(s) 59, 1349, 2545, 2562
AcyI GRCGYC 5 cut(s) 1058, 1692, 2300, 3346, 3576
AflII CTTAAG 1 cut(s) 4414
AflIII ACRYGT 3 cut(s) 133, 412, 2796
AgeI ACCGGT 2 cut(s) 1136, 4823
AjiI CACGTC 2 cut(s) 3745, 4457
AjnI CCWGG 7 cut(s) 1405, 1907, 2997, 3024, 4024, 4131, 5225
AjuI GAANNNNNNNTTGG 6 cut(s) 1790, 1822, 2147, 2179, 2671, 2703
AleI CACNNNNGTG 1 cut(s) 2394
AloI GAACNNNNNNTCC 2 cut(s) 347, 379
Alw21I GWGCWC 4 cut(s) 1368, 2958, 5085, 5368
Alw26I GTCTC 9 cut(s) 9, 733, 1329, 1544, 2015, 3147, 4820, 5247, 5662
AlwI GGATC 7 cut(s) 20, 108, 1660, 3265, 3848, 3861, 5276
Ama87I CYCGRG 4 cut(s) 1361, 3144, 4086, 5766
Aor13HI TCCGGA 1 cut(s) 1575
ApeKI GCWGC 8 cut(s) 455, 683, 1826, 2276, 2279, 3241, 4897, 4910
ArsI GACNNNNNNTTYG 2 cut(s) 5568, 5600
AsiGI ACCGGT 2 cut(s) 1136, 4823
Asp700I GAANNNNTTC 7 cut(s) 511, 585, 1313, 3606, 4098, 4173, 4240
AspA2I CCTAGG 2 cut(s) 2069, 5524
AspLEI GCGC 6 cut(s) 554, 2302, 3348, 3865, 5195, 5473
AspS9I GGNCC 5 cut(s) 71, 557, 2691, 4028, 5647
AsuII TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
AvaI CYCGRG 4 cut(s) 1361, 3144, 4086, 5766
AvaII GGWCC 3 cut(s) 71, 4028, 5647
AvrII CCTAGG 2 cut(s) 2069, 5524
AxyI CCTNAGG 1 cut(s) 5393
BalI TGGCCA 3 cut(s) 1351, 2547, 2564
BamHI GGATCC 1 cut(s) 3853
BanI GGYRCC 3 cut(s) 2299, 3345, 4979
BanII GRGCYC 4 cut(s) 2958, 3838, 5085, 5368
BarI GAAGNNNNNNTAC 4 cut(s) 3054, 3086, 3947, 3979
BauI CACGAG 4 cut(s) 46, 75, 109, 5558
Bbv12I GWGCWC 4 cut(s) 1368, 2958, 5085, 5368
BbvCI CCTCAGC 1 cut(s) 1714
BbvI GCAGC 8 cut(s) 467, 670, 1813, 2263, 2266, 3228, 4897, 4909
BccI CCATC 9 cut(s) 1407, 1518, 2012, 2537, 2941, 3100, 3904, 4834, 5349
BceAI ACGGC 5 cut(s) 74, 332, 598, 872, 4560
BcgI CGANNNNNNTGC 2 cut(s) 4443, 4477
BciT130I CCWGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
BciVI GTATCC 3 cut(s) 2320, 3728, 3903
BclI TGATCA 1 cut(s) 1276
BcoDI GTCTC 9 cut(s) 9, 733, 1329, 1544, 2015, 3147, 4820, 5247, 5662
BfmI CTRYAG 3 cut(s) 773, 1616, 4901
BfoI RGCGCY 3 cut(s) 555, 2303, 3349
BfrI CTTAAG 1 cut(s) 4414
BfuAI ACCTGC 1 cut(s) 4424
BfuI GTATCC 3 cut(s) 2320, 3728, 3903
BglI GCCNNNNNGGC 1 cut(s) 4544
BglII AGATCT 1 cut(s) 2614
BlnI CCTAGG 2 cut(s) 2069, 5524
BlpI GCTNAGC 2 cut(s) 2952, 5665
Bme1390I CCNGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
Bme18I GGWCC 3 cut(s) 71, 4028, 5647
BmeT110I CYCGRG 4 cut(s) 1361, 3144, 4086, 5766
BmgBI CACGTC 2 cut(s) 3745, 4457
BmgT120I GGNCC 5 cut(s) 71, 557, 2691, 4028, 5647
BmrFI CCNGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
BmrI ACTGGG 2 cut(s) 1167, 3625
BmuI ACTGGG 2 cut(s) 1167, 3625
BpmI CTGGAG 5 cut(s) 708, 1158, 3008, 3122, 5269
Bpu10I CCTNAGC 1 cut(s) 1714
Bpu1102I GCTNAGC 2 cut(s) 2952, 5665
Bpu14I TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
BpuEI CTTGAG 6 cut(s) 518, 1087, 1504, 2888, 3569, 4766
Bsa29I ATCGAT 1 cut(s) 5926
BsaAI YACGTR 3 cut(s) 701, 3807, 5348
BsaHI GRCGYC 5 cut(s) 1058, 1692, 2300, 3346, 3576
BsaI GGTCTC 3 cut(s) 1329, 2015, 4820
BsaJI CCNNGG 9 cut(s) 1406, 2069, 2392, 4183, 4539, 4752, 4972, 5226, 5524
BsaWI WCCGGW 6 cut(s) 302, 1136, 1575, 4232, 4326, 4823
BsaXI ACNNNNNCTCC 2 cut(s) 4835, 4865
Bse118I RCCGGY 3 cut(s) 1136, 2427, 4823
Bse21I CCTNAGG 1 cut(s) 5393
Bse3DI GCAATG 3 cut(s) 567, 2733, 3318
BseAI TCCGGA 1 cut(s) 1575
BseBI CCWGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
BseCI ATCGAT 1 cut(s) 5926
BseDI CCNNGG 9 cut(s) 1406, 2069, 2392, 4183, 4539, 4752, 4972, 5226, 5524
BseMI GCAATG 3 cut(s) 567, 2733, 3318
BseRI GAGGAG 2 cut(s) 711, 3935
BseXI GCAGC 8 cut(s) 467, 670, 1813, 2263, 2266, 3228, 4897, 4909
BseYI CCCAGC 2 cut(s) 1528, 2959
BsgI GTGCAG 3 cut(s) 474, 1161, 2369
Bsh1236I CGCG 3 cut(s) 711, 3863, 5471
Bsh1285I CGRYCG 1 cut(s) 4110
BshNI GGYRCC 3 cut(s) 2299, 3345, 4979
BshTI ACCGGT 2 cut(s) 1136, 4823
BshVI ATCGAT 1 cut(s) 5926
BsiEI CGRYCG 1 cut(s) 4110
BsiHKAI GWGCWC 4 cut(s) 1368, 2958, 5085, 5368
BsiHKCI CYCGRG 4 cut(s) 1361, 3144, 4086, 5766
BsiSI CCGG 8 cut(s) 89, 303, 1137, 1576, 2428, 4233, 4327, 4824
BslFI GGGAC 5 cut(s) 2, 1344, 1597, 2076, 5374
BsmAI GTCTC 9 cut(s) 9, 733, 1329, 1544, 2015, 3147, 4820, 5247, 5662
BsmFI GGGAC 5 cut(s) 2, 1344, 1597, 2076, 5374
BsmI GAATGC 5 cut(s) 1337, 1428, 2097, 3228, 4913
Bso31I GGTCTC 3 cut(s) 1329, 2015, 4820
BsoBI CYCGRG 4 cut(s) 1361, 3144, 4086, 5766
Bsp119I TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
Bsp1286I GDGCHC 5 cut(s) 1368, 2958, 3838, 5085, 5368
Bsp13I TCCGGA 1 cut(s) 1575
Bsp1720I GCTNAGC 2 cut(s) 2952, 5665
Bsp19I CCATGG 1 cut(s) 4972
BspDI ATCGAT 1 cut(s) 5926
BspEI TCCGGA 1 cut(s) 1575
BspFNI CGCG 3 cut(s) 711, 3863, 5471
BspHI TCATGA 3 cut(s) 1642, 1695, 5085
BspMAI CTGCAG 1 cut(s) 777
BspMI ACCTGC 1 cut(s) 4424
BspPI GGATC 7 cut(s) 20, 108, 1660, 3265, 3848, 3861, 5276
BspQI GCTCTTC 2 cut(s) 3075, 3597
BspT104I TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
BspT107I GGYRCC 3 cut(s) 2299, 3345, 4979
BspTI CTTAAG 1 cut(s) 4414
BspTNI GGTCTC 3 cut(s) 1329, 2015, 4820
BsrDI GCAATG 3 cut(s) 567, 2733, 3318
BsrFI RCCGGY 3 cut(s) 1136, 2427, 4823
BssAI RCCGGY 3 cut(s) 1136, 2427, 4823
BssECI CCNNGG 9 cut(s) 1406, 2069, 2392, 4183, 4539, 4752, 4972, 5226, 5524
BssNI GRCGYC 5 cut(s) 1058, 1692, 2300, 3346, 3576
BssSI CACGAG 4 cut(s) 46, 75, 109, 5558
BssT1I CCWWGG 6 cut(s) 2069, 2392, 4539, 4752, 4972, 5524
Bst2BI CACGAG 4 cut(s) 46, 75, 109, 5558
Bst2UI CCWGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
BstACI GRCGYC 5 cut(s) 1058, 1692, 2300, 3346, 3576
BstAFI CTTAAG 1 cut(s) 4414
BstAPI GCANNNNNTGC 2 cut(s) 908, 2288
BstBAI YACGTR 3 cut(s) 701, 3807, 5348
BstBI TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
BstDSI CCRYGG 2 cut(s) 4183, 4972
BstENI CCTNNNNNAGG 2 cut(s) 3002, 4136
BstFNI CGCG 3 cut(s) 711, 3863, 5471
BstH2I RGCGCY 3 cut(s) 555, 2303, 3349
BstHHI GCGC 6 cut(s) 554, 2302, 3348, 3865, 5195, 5473
BstMAI GTCTC 9 cut(s) 9, 733, 1329, 1544, 2015, 3147, 4820, 5247, 5662
BstMCI CGRYCG 1 cut(s) 4110
BstNI CCWGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
BstNSI RCATGY 3 cut(s) 137, 2419, 2800
BstSCI CCNGG 7 cut(s) 1405, 1907, 2997, 3024, 4024, 4131, 5225
BstSFI CTRYAG 3 cut(s) 773, 1616, 4901
BstSNI TACGTA 1 cut(s) 701
BstUI CGCG 3 cut(s) 711, 3863, 5471
BstV1I GCAGC 8 cut(s) 467, 670, 1813, 2263, 2266, 3228, 4897, 4909
BstX2I RGATCY 5 cut(s) 113, 1652, 2614, 3270, 3853
BstXI CCANNNNNNTGG 5 cut(s) 1231, 1413, 3534, 3830, 5348
BstYI RGATCY 5 cut(s) 113, 1652, 2614, 3270, 3853
Bsu15I ATCGAT 1 cut(s) 5926
Bsu36I CCTNAGG 1 cut(s) 5393
BsuI GTATCC 3 cut(s) 2320, 3728, 3903
BsuTUI ATCGAT 1 cut(s) 5926
BtgI CCRYGG 2 cut(s) 4183, 4972
BtgZI GCGATG 1 cut(s) 1844
BtrI CACGTC 2 cut(s) 3745, 4457
BtsIMutI CAGTG 9 cut(s) 366, 1501, 1857, 3427, 4564, 4726, 5038, 5169, 5679
BveI ACCTGC 1 cut(s) 4424
CciI TCATGA 3 cut(s) 1642, 1695, 5085
CfoI GCGC 6 cut(s) 554, 2302, 3348, 3865, 5195, 5473
Cfr10I RCCGGY 3 cut(s) 1136, 2427, 4823
Cfr13I GGNCC 5 cut(s) 71, 557, 2691, 4028, 5647
ClaI ATCGAT 1 cut(s) 5926
CseI GACGC 4 cut(s) 1047, 1346, 3584, 4744
CspAI ACCGGT 2 cut(s) 1136, 4823
CspCI CAANNNNNGTGG 4 cut(s) 2292, 2327, 5303, 5338
DinI GGCGCC 2 cut(s) 2301, 3347
DraI TTTAAA 1 cut(s) 2044
DrdI GACNNNNNNGTC 1 cut(s) 5654
DseDI GACNNNNNNGTC 1 cut(s) 5654
EaeI YGGCCR 4 cut(s) 59, 1349, 2545, 2562
Ecl136II GAGCTC 3 cut(s) 2956, 5083, 5366
Eco105I TACGTA 1 cut(s) 701
Eco130I CCWWGG 6 cut(s) 2069, 2392, 4539, 4752, 4972, 5524
Eco24I GRGCYC 4 cut(s) 2958, 3838, 5085, 5368
Eco31I GGTCTC 3 cut(s) 1329, 2015, 4820
Eco32I GATATC 1 cut(s) 3079
Eco47I GGWCC 3 cut(s) 71, 4028, 5647
Eco53kI GAGCTC 3 cut(s) 2956, 5083, 5366
Eco81I CCTNAGG 1 cut(s) 5393
Eco88I CYCGRG 4 cut(s) 1361, 3144, 4086, 5766
EcoICRI GAGCTC 3 cut(s) 2956, 5083, 5366
EcoNI CCTNNNNNAGG 2 cut(s) 3002, 4136
EcoO109I RGGNCCY 2 cut(s) 71, 5647
EcoRI GAATTC 1 cut(s) 3123
EcoRII CCWGG 7 cut(s) 1405, 1907, 2997, 3024, 4024, 4131, 5225
EcoRV GATATC 1 cut(s) 3079
EcoT14I CCWWGG 6 cut(s) 2069, 2392, 4539, 4752, 4972, 5524
EcoT22I ATGCAT 7 cut(s) 666, 913, 2176, 2461, 3230, 3787, 4466
EcoT38I GRGCYC 4 cut(s) 2958, 3838, 5085, 5368
EgeI GGCGCC 2 cut(s) 2301, 3347
EheI GGCGCC 2 cut(s) 2301, 3347
ErhI CCWWGG 6 cut(s) 2069, 2392, 4539, 4752, 4972, 5524
FaqI GGGAC 5 cut(s) 2, 1344, 1597, 2076, 5374
FauI CCCGC 2 cut(s) 567, 704
FauNDI CATATG 3 cut(s) 1046, 1114, 1180
FbaI TGATCA 1 cut(s) 1276
FblI GTMKAC 4 cut(s) 4828, 5103, 5466, 5520
FriOI GRGCYC 4 cut(s) 2958, 3838, 5085, 5368
GlaI GCGC 6 cut(s) 553, 2301, 3347, 3864, 5194, 5472
GsaI CCCAGC 2 cut(s) 1532, 2963
GsuI CTGGAG 5 cut(s) 708, 1158, 3008, 3122, 5269
HaeII RGCGCY 3 cut(s) 555, 2303, 3349
HapII CCGG 8 cut(s) 89, 303, 1137, 1576, 2428, 4233, 4327, 4824
HgaI GACGC 4 cut(s) 1047, 1346, 3584, 4744
HhaI GCGC 6 cut(s) 554, 2302, 3348, 3865, 5195, 5473
Hin1I GRCGYC 5 cut(s) 1058, 1692, 2300, 3346, 3576
Hin6I GCGC 6 cut(s) 552, 2300, 3346, 3863, 5193, 5471
HinP1I GCGC 6 cut(s) 552, 2300, 3346, 3863, 5193, 5471
HincII GTYRAC 3 cut(s) 3294, 3988, 4703
HindII GTYRAC 3 cut(s) 3294, 3988, 4703
HindIII AAGCTT 2 cut(s) 1727, 5871
HpaI GTTAAC 2 cut(s) 3294, 3988
HpaII CCGG 8 cut(s) 89, 303, 1137, 1576, 2428, 4233, 4327, 4824
Hpy99I CGWCG 4 cut(s) 145, 1063, 4458, 5333
Hsp92I GRCGYC 5 cut(s) 1058, 1692, 2300, 3346, 3576
HspAI GCGC 6 cut(s) 552, 2300, 3346, 3863, 5193, 5471
KasI GGCGCC 2 cut(s) 2299, 3345
Kpn2I TCCGGA 1 cut(s) 1575
Ksp22I TGATCA 1 cut(s) 1276
KspAI GTTAAC 2 cut(s) 3294, 3988
LguI GCTCTTC 2 cut(s) 3075, 3597
LmnI GCTCC 9 cut(s) 145, 886, 1177, 1578, 2961, 3141, 3397, 3833, 5363
Lsp1109I GCAGC 8 cut(s) 467, 670, 1813, 2263, 2266, 3228, 4897, 4909
MfeI CAATTG 3 cut(s) 693, 1974, 2283
MflI RGATCY 5 cut(s) 113, 1652, 2614, 3270, 3853
MhlI GDGCHC 5 cut(s) 1368, 2958, 3838, 5085, 5368
MlsI TGGCCA 3 cut(s) 1351, 2547, 2564
MluNI TGGCCA 3 cut(s) 1351, 2547, 2564
Mly113I GGCGCC 2 cut(s) 2300, 3346
MlyI GAGTC 5 cut(s) 233, 1026, 1546, 4332, 5845
MmeI TCCRAC 6 cut(s) 123, 3921, 4223, 4471, 4833, 5341
Mox20I TGGCCA 3 cut(s) 1351, 2547, 2564
Mph1103I ATGCAT 7 cut(s) 666, 913, 2176, 2461, 3230, 3787, 4466
MroI TCCGGA 1 cut(s) 1575
MroXI GAANNNNTTC 7 cut(s) 511, 585, 1313, 3606, 4098, 4173, 4240
MscI TGGCCA 3 cut(s) 1351, 2547, 2564
MslI CAYNNNNRTG 8 cut(s) 1229, 2335, 2394, 3380, 4347, 4892, 4977, 5346
Msp20I TGGCCA 3 cut(s) 1351, 2547, 2564
MspA1I CMGCKG 2 cut(s) 2656, 3241
MspCI CTTAAG 1 cut(s) 4414
MspI CCGG 8 cut(s) 89, 303, 1137, 1576, 2428, 4233, 4327, 4824
MspR9I CCNGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
MunI CAATTG 3 cut(s) 693, 1974, 2283
Mva1269I GAATGC 5 cut(s) 1337, 1428, 2097, 3228, 4913
MvaI CCWGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
MvnI CGCG 3 cut(s) 711, 3863, 5471
NarI GGCGCC 2 cut(s) 2300, 3346
NcoI CCATGG 1 cut(s) 4972
NdeI CATATG 3 cut(s) 1046, 1114, 1180
NmeAIII GCCGAG 3 cut(s) 3040, 3319, 3698
NmuCI GTSAC 8 cut(s) 541, 1948, 2109, 3116, 4285, 4577, 5243, 5630
NsiI ATGCAT 7 cut(s) 666, 913, 2176, 2461, 3230, 3787, 4466
NspI RCATGY 3 cut(s) 137, 2419, 2800
NspV TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
OliI CACNNNNGTG 1 cut(s) 2394
PaeR7I CTCGAG 2 cut(s) 4086, 5766
PagI TCATGA 3 cut(s) 1642, 1695, 5085
PciI ACATGT 2 cut(s) 133, 2796
PciSI GCTCTTC 2 cut(s) 3075, 3597
PctI GAATGC 5 cut(s) 1337, 1428, 2097, 3228, 4913
PdmI GAANNNNTTC 7 cut(s) 511, 585, 1313, 3606, 4098, 4173, 4240
PflMI CCANNNNNTGG 5 cut(s) 286, 890, 1108, 1181, 5685
PfoI TCCNGGA 1 cut(s) 4024
PinAI ACCGGT 2 cut(s) 1136, 4823
Ple19I CGATCG 1 cut(s) 4110
PleI GAGTC 5 cut(s) 232, 1026, 1546, 4331, 5845
PluTI GGCGCC 2 cut(s) 2303, 3349
PpsI GAGTC 5 cut(s) 232, 1026, 1546, 4331, 5845
Ppu21I YACGTR 3 cut(s) 701, 3807, 5348
PpuMI RGGWCCY 2 cut(s) 71, 5647
PscI ACATGT 2 cut(s) 133, 2796
PsiI TTATAA 2 cut(s) 1733, 4440
Psp124BI GAGCTC 3 cut(s) 2958, 5085, 5368
Psp1406I AACGTT 1 cut(s) 4600
Psp5II RGGWCCY 2 cut(s) 71, 5647
Psp6I CCWGG 7 cut(s) 1405, 1907, 2997, 3024, 4024, 4131, 5225
PspFI CCCAGC 2 cut(s) 1528, 2959
PspGI CCWGG 7 cut(s) 1405, 1907, 2997, 3024, 4024, 4131, 5225
PspPI GGNCC 5 cut(s) 71, 557, 2691, 4028, 5647
PspPPI RGGWCCY 2 cut(s) 71, 5647
PspXI VCTCGAGB 2 cut(s) 4086, 5766
PstI CTGCAG 1 cut(s) 777
PsuI RGATCY 5 cut(s) 113, 1652, 2614, 3270, 3853
PvuI CGATCG 1 cut(s) 4110
RseI CAYNNNNRTG 8 cut(s) 1229, 2335, 2394, 3380, 4347, 4892, 4977, 5346
SacI GAGCTC 3 cut(s) 2958, 5085, 5368
SapI GCTCTTC 2 cut(s) 3075, 3597
Sau96I GGNCC 5 cut(s) 71, 557, 2691, 4028, 5647
SchI GAGTC 5 cut(s) 233, 1026, 1546, 4332, 5845
ScrFI CCNGG 7 cut(s) 1407, 1909, 2999, 3026, 4026, 4133, 5227
SduI GDGCHC 5 cut(s) 1368, 2958, 3838, 5085, 5368
SfcI CTRYAG 3 cut(s) 773, 1616, 4901
SfoI GGCGCC 2 cut(s) 2301, 3347
Sfr274I CTCGAG 2 cut(s) 4086, 5766
SfuI TTCGAA 5 cut(s) 1338, 1686, 2404, 3610, 4177
SinI GGWCC 3 cut(s) 71, 4028, 5647
SlaI CTCGAG 2 cut(s) 4086, 5766
SmiMI CAYNNNNRTG 8 cut(s) 1229, 2335, 2394, 3380, 4347, 4892, 4977, 5346
SmlI CTYRAG 9 cut(s) 533, 1066, 1519, 2867, 3548, 4086, 4414, 4745, 5766
SmoI CTYRAG 9 cut(s) 533, 1066, 1519, 2867, 3548, 4086, 4414, 4745, 5766
SnaBI TACGTA 1 cut(s) 701
SspDI GGCGCC 2 cut(s) 2299, 3345
SspI AATATT 1 cut(s) 4365
SstI GAGCTC 3 cut(s) 2958, 5085, 5368
StyD4I CCNGG 7 cut(s) 1405, 1907, 2997, 3024, 4024, 4131, 5225
StyI CCWWGG 6 cut(s) 2069, 2392, 4539, 4752, 4972, 5524
TaqII GACCGA 1 cut(s) 4283
TatI WGTACW 3 cut(s) 2793, 2799, 2835
TauI GCSGC 3 cut(s) 2656, 3346, 3773
TscAI CASTG 9 cut(s) 373, 1501, 1857, 3427, 4564, 4726, 5038, 5169, 5686
TseFI GTSAC 8 cut(s) 541, 1948, 2109, 3116, 4285, 4577, 5243, 5630
TseI GCWGC 8 cut(s) 455, 683, 1826, 2276, 2279, 3241, 4897, 4910
Tsp45I GTSAC 8 cut(s) 541, 1948, 2109, 3116, 4285, 4577, 5243, 5630
TspGWI ACGGA 6 cut(s) 1469, 2675, 3228, 3893, 4566, 4660
TspRI CASTG 9 cut(s) 373, 1501, 1857, 3427, 4564, 4726, 5038, 5169, 5686
Van91I CCANNNNNTGG 5 cut(s) 286, 890, 1108, 1181, 5685
Vha464I CTTAAG 1 cut(s) 4414
VpaK11BI GGWCC 3 cut(s) 71, 4028, 5647
XagI CCTNNNNNAGG 2 cut(s) 3002, 4136
XceI RCATGY 3 cut(s) 137, 2419, 2800
XcmI CCANNNNNNNNNTGG 3 cut(s) 1521, 2572, 3845
XhoI CTCGAG 2 cut(s) 4086, 5766
XmaJI CCTAGG 2 cut(s) 2069, 5524
XmiI GTMKAC 4 cut(s) 4828, 5103, 5466, 5520
XmnI GAANNNNTTC 7 cut(s) 511, 585, 1313, 3606, 4098, 4173, 4240
ZraI GACGTC 1 cut(s) 1693
Zsp2I ATGCAT 7 cut(s) 666, 913, 2176, 2461, 3230, 3787, 4466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.