MD11G1276200.v1.1

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016003
Physical Location & Seq
Reverse (-)
39308476 .. 39320179
11704 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1276200.v1.1.491

Sequence Viewer

Length: 5859 bp
ATGTCTGAGATTGTGGTTGCAGAGCCTATCTATGAAGGCGACCTGGAAGAGCCGCAAAGGCCCGTTACACGGGCCATTACTATGGGCTCTACAAGTTATCACAATCATGTTCCCGAACCCTTTGAGAGCGAGAGATTGCCTGCTACTCTGGCTTCTGAAATCCAGAGGTTTCTGCGTGTTGCAAATTTGATCGAAACCGAGGAGCCCCGTATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGCAATTCCACTGGTCGGGGTGTTCGACAATTCAAAACTTCCCTTCTCCAGCGACTTGAACAGGATGAAGAAACTACAATAACAAAACGAAAGGAAAAGAGCGATATACGTGAGCTGAGACGTGTCTATCATGATTACAAAGAGTACATCATTAAAAATGATAGAGCGTTTCATTTGGAGAATAGACACAGAGAGAAGTTGATAAATGCACGTAGAATCGGCTCTGTTTTGTTTGAAGTTCTAAAGACAGTTTCAAATACAAACCCTCAGGCACTTGCTAATAGAGGTGGCATCCAGAACAAATCTAATGATCTCTTCGTGCCATATAATATTCTACCACTCGATCATGGAGGTATTCAGCAAGCAATTATGAAACTACCTGAGATTAAGGCCGCACTAGCTGCTATTCGCAATATTCGTGGTATACCTTCGGCCAATGATTTTCAGAAACATGGGGATTTCATTGATATGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAGGGAAATGTCACCAATCAGAGGGAACATCTACTTCTACTCCTTGCTAACATTCACATACGAAAAACTCATAAGCAGGCATCTGTCTCAAAGTTAGAAGATGGTGCAGTGGATGAGTTAATGCGCAAATTTTTTAAAAATTATACAAACTGGTGCAAATTTTTTGGTAGGAAAAGCAACATCCGGTTGCCTTATGTGAAACAAGAAGCACAACAGTATAAACTTTTATATCTAGCGCTTTACCTTCTTATCTGGGGGGAGGCTGCAAATCTCCGATTCATGCCAGAGTGTCTATGTTATATTTTTCACCATATGGCGTATGAGTTACATGGTACACTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAAGTCATGCCAGCATATGGAGGCCAGCCCGAGTCTTTCCTCAACAATGTAGTTACCCCCATATACACGGTTATAAGCGAGGAAGCCAAGAAAAGCAAAGGTGGGACAACTGACCATGCCATGTGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTCCTGATTGTTTCGAAATAGGTTGGCCCATGCGTCTAGACCATGATTTCTTCTGCATACAACCTTCAAAGAAACCCAAACCTAAGAAGGCTTCAGTATCCACTGGTTCTGTAGAAGAAAGGAGGGAAGACGGAGAGGAAGTGGAAGCAGGGGCTACTACTAAAGAAGAAGATCGCGAGCCCCAATGGTTAGGGAAAACAAATTTTGTAGAAGTCCGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTCTTTCCCTTCAGGCATTGATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAACTGTTTGACAAGGTTATATTCGAGGACGTCATGAGCATATTTGTCACATCTGCGTTTCTTAAACTCTTACGAGCAATGCTCGACATTGTATTTACATGGAAAGCCAGACAAACAATGGAATTCTCTGGAAAAGTAAGACATGTTATGAAGCTGGTTGTTGCAGCGATATGGACTATAGTTCTTCCTGTTTATTATGGAAATTCTAGAAGAAAATATACTTGTTATCCTGCACGATATGGAAGCTGGCTTCAGGAGTGGTGTTTCTCCTCCTATATGGTTGCAGTTGCAATTTATTTGACGACTAACGCAGTTGAGATGGTGCTCTTTTTGGTTCCTTCTATTCGCAAATACATCGAGATCTCAAATTACCGAATATGCACTATTTTGTCTTGGTGGACACAGCCTGGATTATATATTGGTCGTGGGATGCAAGAAAGCCAGCTTTCAGTATTGAAGTATACAGTGTTTTGGGTGCTAGTACTACTGAGCAAATTTTCTTTCAGCTACTTCTTCGAGATAAAACCGCTCATAAAACCAACGAAAAAGATCATTAAACTTAGTGTGAAACATTATGAGTGGCACGAGCTTTTTCCAACAGTTCAGAATAATGCCGGTGTAATTGTTGCTATATGGGCTCCAATAGTAGTTGTGTATTTTATGGACACTCAGATATGGTATTCTGTTTACTGCACAATCTTTGGTGGAGTCTATGGCATATTGCACCACCTTGGTGAGATTCGGACGTTGGGGATGTTGAGAAGTCGGTTTCACACTTTGCCTTCTGCATTCAATGTTTCCCTTATCCCACCCTCATCAAGAAATGATCAAAAGAGAAAAAACGGTTTCTTTCACAATAAATTCAAAAAGGTCTCAAAGACTGAAAACAATGGTCTTGCTAAGTTTGTTTTAGTTTGGAACCAAATTATCAATAGCTTTCGAACGGAGGACTTGATAAACAACAGGGAGTTGGATTTGATGAGTATGCCTATGTCATCAGAATTATTTTCTGGCATTGTTCGTTGGCCTGTTTTTCTTCTTGCGAATAAGTTTTCAACAGCGCTCAGTATCGCAAAGGATTTTGTCGGGAAGGATGAGATTCTTATCAGAAAAATAAAAAAAGACGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCCTTGAAGTACATCCTCGAAATCCTCGTTGTTGGTCGTCTTGAAAAAAGTATTGTATCCGCCGTGCTGACTGAAATTGAAGAAAGCATCGCAAGGTCAAGTCTTCTTGAGGACTTACGGATGACTGAACTTCCACATCTTCTCGCCAAATGTATCGAGTTGATTGAACTTCTGGTTGAGGGAAACGAAGAGCATCACAGTAAAGTTGTGAAGATTCTTCAAGATATATTTGAACTTGTAACCAACGATATGATGACTAATGGCTCCAGGATACTGGATTTGCTGAATTCATTCCAACAAATCGACATGGACTTTGCTGATTTTACTAGAACTATTGAACCAGAATTATTTGGATCTGCTGATGACAAGAACTCCATCCATTTCCCTTTGCCAGACGACAGTGCTTCTCTCATTGAGCAGATCAAGCGTTTCCATCTGTTGCTTACTGTCAAAGATACAGCATTGGACATTCCAACTAACTTAGAGGCTCGAAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAACATGCCCGGCGCACCCAAAGTCCGCAATATGCTGCCTTTCTGTGTTATGACACCACACTTCATGGAGGATATTAATTTTTCAATGAAGGAGCTTCATTCGAGCCAACAAGAGGTCTCCATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCTTGGAGCGCATGGGTTGTGAAAACTTGGATGGACTGAAAGAGGAAAGCAAGGAGGAAGAGCTTAGGAATTGGGCTTCCTACCGGGGCCAAACGCTAAGCAGAACAGTTCGAGGAATGATGTACTATAGAGAAGCCTTAAAATTACAAGCATTCCTCGACGTGGCTGAAGAAGAAGATATTCTTGAAGGTTATGACGCCGTTGAAAGTAGAAATCGCGAGTTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTACTTACTTGTCAATTGTTTGGATCACAAAAAGCTTCTGGAGATCCACATGCACAAGACATTATTGACTTGATGAAAAGACACCCATCTCTCCGCGTTGCTTACGTTGAGGAGAAAGAAGTGATAGTTGGAAAAAAGCCTCAGAAAGTTTATTCTTCTGTATTGGTTAAAGCTGTCAATGATTTTGATCAGGAAATATATCGCATAAAGCTTCCTGGTCCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAATCACGGGATAATTTTCACTCGTGGTGAAGCTCTCCAGACCATTGATATGAACCAAGATAGTTATCTGGAAGAAGCATTCAAAATGAGAAACGTTCTGCAAGAATTTCTTCAGAGCCAAGGACGGAGACCTCCTACGCTACTTGGTTTGAGGGAACACATATTCACAGGAAGTGTTTCTTCTCTTGCTTGGTTCATGTCGTATCAAGAGACCAGCTTCGTCACCATTGGTCAAAGGCTTCTTGCTAATCCCCTCAGAGTACGGTTCCATTATGGACATCCGGATGTATTTGACAGACTATTTCACATCACAAGAGGAGGCATAAGCAAAGCATCAAAGACAATTAACCTAAGCGAGGATGTTTTTGCTGGATTTAACTGTACTTTACGACGCGGATGTATAACTTATCACGAGTACATGCAAGTTGGCAAGGGGCGTGATGTAAGTCTGAACCAGATATCAAAGTTCGAAGCTAAGGTTGCTAACGGAAACAGTGAACAGACTATAAGCCGTGACATATACCACCTTGGACGCCAGTTTGATTTCTTCCGTATGCTATCATGTTATTTCACAACCATTGGGTTTTACTTTAGTAGTCTGATGTCAGTTATTGGAATATACGTCTTCCTTTACGGACAGTTATACCTTGTCCTCAGTGGCTTGGAAAGAGTGATTATAGTTGAAGCGAGACTGCAGAACATACAATCCTTGGAAACAGCTCTTGCATCGCAGTCATTTATACAGCTAGGCCTACTAACAGGATTACCAATGGTGATGGAAATTGGGCTAGAGAAGGGATTCCTTAACGCAATTAAAGATTTTGTCCTGATGCAGTTGCAGCTGGCTTCTGTTTTCTTCACTTTCTCCTTCGGCACAAAAATACATTATTATGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTTGTTGTGTTCCATACTAGCTTCACTGAGAACTACAGGTTATATTCAAGAAGCCACTTTGTGAAAGGATTCGAGTTGTTACTCCTGTTGATTGTCTACGATTTGTTTAGGCGGTCATATCAGAGCAGCATGGTGTATGTATTAATCACTTACTCCATTTGGTTCATGTCAATCACTTGGTTGTTTGCTCCATTTCTTTTTAATCCCTCCGGATTCAGTTGGGAGAAGATAGTAGACGACTGGAAAGATTGGAACAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCAACAAGATAAAAGTTGGCAGTCTTGGTGGATGGATGAGCAAGATCATCTTCGCCGTTCAGGGACGACTTCTAGGTTGCTTGAAATACTCCTTTCCGTTCGATTTTTCCTGTATCAGTATGGTCTTGTCTACCACCTTGACATCTCCCAGAATAGCAGGAATTTTCTGGTATATTTGCTTTCATGGATTGTGATCCTCGCAGTTTTCTTGTTAGTCAAGGCAGTGAATCTCGGAAGGAAACAGTTCAGCGCTAAATATCACCTCGTGTTTAGGCTGTTCAAGGCATTCCTCTTCTTGGGTGTTCTTGCTGTCATACTTGCTCTCTATTTTATATGCAAACTCTCCTGGAAGGACTTAGTCGTCTGCTGTCTAGCATTTTTTCCGACAGGCTGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAATACTGGATTCTGGGAATTCACCAGAGTACTCGCTAAGACTTATGATTACGGAATGGGTGTTGCTCTCTTTGCACCTATCGCGGTCTTTTCATGGCTTCCGATTATATCAGCATTCCAGACTCGTTTCCTGTTCAACGAGGCGTTCAACAGGCACTTACAGATCCAACCAATTCTTGCAGGGAAGAAGAAGAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1953

Amino Acids

226.5

Weight (kDa)

8.67

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 54 - 177 2.2e-09 Vta1 like
FKS1_dom1 PF14288 329 - 440 3.6e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 882 - 1076 3.7e-49 Callose synthase 1 domain
Glucan_synthase PF02364 1083 - 1769 9.9e-218 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1208
AasI GACNNNNNNGTC 1 cut(s) 5582
AatII GACGTC 1 cut(s) 1650
Acc16I TGCGCA 1 cut(s) 890
AccB7I CCANNNNNTGG 3 cut(s) 1151, 1603, 3968
AccBSI CCGCTC 1 cut(s) 2146
AccI GTMKAC 5 cut(s) 685, 2078, 5031, 5169, 5352
AccII CGCG 5 cut(s) 1470, 3690, 3834, 4386, 5741
AccIII TCCGGA 2 cut(s) 4273, 5144
AclI AACGTT 1 cut(s) 4086
AclWI GGATC 6 cut(s) 3139, 3769, 3776, 5204, 5410, 5815
AcoI YGGCCR 2 cut(s) 693, 4885
AcuI CTGAAG 6 cut(s) 728, 1371, 1556, 1853, 3660, 4088
AcyI GRCGYC 3 cut(s) 1647, 3669, 4525
AdeI CACNNNGTG 2 cut(s) 4996, 5488
AfeI AGCGCT 3 cut(s) 1002, 2679, 5473
AflIII ACRYGT 3 cut(s) 382, 1759, 2748
AjiI CACGTC 2 cut(s) 383, 3634
AjnI CCWGG 5 cut(s) 42, 2023, 3044, 3952, 5567
AjuI GAANNNNNNNTTGG 4 cut(s) 3458, 3490, 3849, 3881
AleI CACNNNNGTG 1 cut(s) 2349
AloI GAACNNNNNNTCC 2 cut(s) 4682, 4714
Alw21I GWGCWC 1 cut(s) 1944
Alw26I GTCTC 8 cut(s) 373, 856, 1299, 2494, 3427, 4114, 4196, 4675
AlwI GGATC 6 cut(s) 3139, 3769, 3776, 5204, 5410, 5815
Ama87I CYCGRG 1 cut(s) 1163
Aor13HI TCCGGA 2 cut(s) 4273, 5144
Aor51HI AGCGCT 3 cut(s) 1002, 2679, 5473
ApeKI GCWGC 7 cut(s) 662, 1028, 1781, 3340, 4831, 5061, 5203
ArsI GACNNNNNNTTYG 2 cut(s) 3074, 3106
AseI ATTAAT 2 cut(s) 3381, 5078
Asp700I GAANNNNTTC 8 cut(s) 3068, 3470, 3651, 4101, 4168, 4790, 5003, 5465
AspLEI GCGC 6 cut(s) 891, 1003, 2680, 3320, 3483, 5474
AspS9I GGNCC 7 cut(s) 60, 72, 259, 1320, 3559, 3956, 5646
AsuC2I CCSGG 2 cut(s) 3315, 3557
AsuHPI GGTGA 8 cut(s) 769, 1064, 2363, 4031, 4207, 4777, 5474, 5671
AsuII TTCGAA 3 cut(s) 1308, 2557, 4461
AvaI CYCGRG 1 cut(s) 1163
AvaII GGWCC 2 cut(s) 259, 3956
AxyI CCTNAGG 1 cut(s) 528
BanII GRGCYC 4 cut(s) 89, 207, 1476, 2257
BarI GAAGNNNNNNTAC 4 cut(s) 1819, 1851, 2756, 2788
BauI CACGAG 4 cut(s) 2201, 4014, 4403, 5486
BbsI GAAGAC 4 cut(s) 1428, 2873, 3277, 4609
Bbv12I GWGCWC 1 cut(s) 1944
BbvI GCAGC 7 cut(s) 649, 1015, 1793, 3327, 4843, 5073, 5215
BccI CCATC 9 cut(s) 860, 1930, 3161, 3219, 3434, 3497, 3832, 4762, 5248
BceAI ACGGC 4 cut(s) 2825, 3656, 4488, 5262
BcgI CGANNNNNNTGC 4 cut(s) 1954, 1988, 3680, 3714
BciT130I CCWGG 5 cut(s) 44, 2025, 3046, 3954, 5569
BciVI GTATCC 3 cut(s) 1402, 2845, 3042
BclI TGATCA 2 cut(s) 2443, 3925
BcnI CCSGG 2 cut(s) 3315, 3557
BcoDI GTCTC 8 cut(s) 373, 856, 1299, 2494, 3427, 4114, 4196, 4675
BfmI CTRYAG 5 cut(s) 1404, 1794, 3598, 4685, 4969
BfoI RGCGCY 3 cut(s) 1004, 2681, 5475
BfuI GTATCC 3 cut(s) 1402, 2845, 3042
BglI GCCNNNNNGGC 1 cut(s) 58
BglII AGATCT 1 cut(s) 1977
BlpI GCTNAGC 2 cut(s) 3569, 3702
BmcAI AGTACT 3 cut(s) 749, 2100, 5688
Bme1390I CCNGG 7 cut(s) 44, 2025, 3046, 3315, 3557, 3954, 5569
Bme18I GGWCC 2 cut(s) 259, 3956
BmeT110I CYCGRG 1 cut(s) 1163
BmgBI CACGTC 2 cut(s) 383, 3634
BmgT120I GGNCC 7 cut(s) 60, 72, 259, 1320, 3559, 3956, 5646
BmiI GGNNCC 8 cut(s) 204, 1953, 2256, 2537, 3043, 3560, 4259, 5648
BmrFI CCNGG 7 cut(s) 44, 2025, 3046, 3315, 3557, 3954, 5569
BmrI ACTGGG 1 cut(s) 4935
BmsI GCATC 9 cut(s) 561, 854, 2037, 2874, 2980, 3700, 4334, 4727, 4812
BmuI ACTGGG 1 cut(s) 4935
BpiI GAAGAC 4 cut(s) 1428, 2873, 3277, 4609
BplI GAGNNNNNCTC 2 cut(s) 1683, 1715
BpmI CTGGAG 4 cut(s) 293, 3028, 3798, 4013
Bpu10I CCTNAGC 3 cut(s) 3536, 4343, 4467
Bpu1102I GCTNAGC 2 cut(s) 3569, 3702
Bpu14I TTCGAA 3 cut(s) 1308, 2557, 4461
BpuEI CTTGAG 1 cut(s) 2906
BpuMI CCSGG 2 cut(s) 3315, 3557
BsaAI YACGTR 2 cut(s) 371, 473
BsaBI GATNNNNATC 4 cut(s) 2720, 3924, 4056, 5414
BsaHI GRCGYC 3 cut(s) 1647, 3669, 4525
BsaI GGTCTC 5 cut(s) 1299, 2494, 3427, 4114, 4196
BsaJI CCNNGG 6 cut(s) 198, 2347, 3556, 4111, 4519, 4701
BsaWI WCCGGW 3 cut(s) 948, 4273, 5144
BsaXI ACNNNNNCTCC 6 cut(s) 789, 819, 1419, 1449, 3134, 3164
Bse118I RCCGGY 1 cut(s) 2231
Bse1I ACTGG 9 cut(s) 277, 920, 1111, 1402, 3057, 4528, 4930, 5180, 5668
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 1701
Bse8I GATNNNNATC 4 cut(s) 2720, 3924, 4056, 5414
BseAI TCCGGA 2 cut(s) 4273, 5144
BseBI CCWGG 5 cut(s) 44, 2025, 3046, 3954, 5569
BseDI CCNNGG 6 cut(s) 198, 2347, 3556, 4111, 4519, 4701
BseJI GATNNNNATC 4 cut(s) 2720, 3924, 4056, 5414
BseMI GCAATG 1 cut(s) 1701
BseNI ACTGG 9 cut(s) 277, 920, 1111, 1402, 3057, 4528, 4930, 5180, 5668
BseRI GAGGAG 4 cut(s) 215, 1876, 3863, 4323
BseXI GCAGC 7 cut(s) 649, 1015, 1793, 3327, 4843, 5073, 5215
BseYI CCCAGC 1 cut(s) 5613
BsgI GTGCAG 3 cut(s) 891, 1833, 2293
Bsh1236I CGCG 5 cut(s) 1470, 3690, 3834, 4386, 5741
BsiHKAI GWGCWC 1 cut(s) 1944
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 6 cut(s) 949, 2232, 3315, 3556, 4274, 5145
BslFI GGGAC 2 cut(s) 1252, 5299
BsmAI GTCTC 8 cut(s) 373, 856, 1299, 2494, 3427, 4114, 4196, 4675
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 2 cut(s) 1252, 5299
BsmI GAATGC 5 cut(s) 2405, 3623, 4070, 5507, 5771
Bso31I GGTCTC 5 cut(s) 1299, 2494, 3427, 4114, 4196
BsoBI CYCGRG 1 cut(s) 1163
Bsp119I TTCGAA 3 cut(s) 1308, 2557, 4461
Bsp1286I GDGCHC 5 cut(s) 89, 207, 1476, 1944, 2257
Bsp13I TCCGGA 2 cut(s) 4273, 5144
Bsp1720I GCTNAGC 2 cut(s) 3569, 3702
Bsp68I TCGCGA 2 cut(s) 1470, 3690
BspEI TCCGGA 2 cut(s) 4273, 5144
BspFNI CGCG 5 cut(s) 1470, 3690, 3834, 4386, 5741
BspHI TCATGA 2 cut(s) 391, 1650
BspLI GGNNCC 8 cut(s) 204, 1953, 2256, 2537, 3043, 3560, 4259, 5648
BspMAI CTGCAG 1 cut(s) 4689
BspPI GGATC 6 cut(s) 3139, 3769, 3776, 5204, 5410, 5815
BspQI GCTCTTC 3 cut(s) 42, 2961, 3525
BspT104I TTCGAA 3 cut(s) 1308, 2557, 4461
BspTNI GGTCTC 5 cut(s) 1299, 2494, 3427, 4114, 4196
BsrBI CCGCTC 1 cut(s) 2146
BsrDI GCAATG 1 cut(s) 1701
BsrFI RCCGGY 1 cut(s) 2231
BsrI ACTGG 9 cut(s) 277, 920, 1111, 1402, 3057, 4528, 4930, 5180, 5668
BssAI RCCGGY 1 cut(s) 2231
BssECI CCNNGG 6 cut(s) 198, 2347, 3556, 4111, 4519, 4701
BssNAI GTATAC 2 cut(s) 686, 2079
BssNI GRCGYC 3 cut(s) 1647, 3669, 4525
BssSI CACGAG 4 cut(s) 2201, 4014, 4403, 5486
BssT1I CCWWGG 4 cut(s) 2347, 4111, 4519, 4701
Bst1107I GTATAC 2 cut(s) 686, 2079
Bst2BI CACGAG 4 cut(s) 2201, 4014, 4403, 5486
Bst2UI CCWGG 5 cut(s) 44, 2025, 3046, 3954, 5569
Bst6I CTCTTC 5 cut(s) 42, 581, 2961, 3525, 5519
BstACI GRCGYC 3 cut(s) 1647, 3669, 4525
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBAI YACGTR 2 cut(s) 371, 473
BstBI TTCGAA 3 cut(s) 1308, 2557, 4461
BstENI CCTNNNNNAGG 1 cut(s) 4346
BstFNI CGCG 5 cut(s) 1470, 3690, 3834, 4386, 5741
BstH2I RGCGCY 3 cut(s) 1004, 2681, 5475
BstHHI GCGC 6 cut(s) 891, 1003, 2680, 3320, 3483, 5474
BstMAI GTCTC 8 cut(s) 373, 856, 1299, 2494, 3427, 4114, 4196, 4675
BstNI CCWGG 5 cut(s) 44, 2025, 3046, 3954, 5569
BstNSI RCATGY 7 cut(s) 1596, 1763, 2752, 3313, 3442, 3791, 4414
BstSCI CCNGG 7 cut(s) 42, 2023, 3044, 3313, 3555, 3952, 5567
BstSFI CTRYAG 5 cut(s) 1404, 1794, 3598, 4685, 4969
BstUI CGCG 5 cut(s) 1470, 3690, 3834, 4386, 5741
BstV1I GCAGC 7 cut(s) 649, 1015, 1793, 3327, 4843, 5073, 5215
BstV2I GAAGAC 4 cut(s) 1428, 2873, 3277, 4609
BstX2I RGATCY 4 cut(s) 1977, 3131, 3781, 5820
BstXI CCANNNNNNTGG 3 cut(s) 82, 3052, 3462
BstYI RGATCY 4 cut(s) 1977, 3131, 3781, 5820
BstZ17I GTATAC 2 cut(s) 686, 2079
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 3 cut(s) 1402, 2845, 3042
BtgZI GCGATG 2 cut(s) 2851, 4704
BtrI CACGTC 2 cut(s) 383, 3634
BtsI GCAGTG 2 cut(s) 879, 5451
BtuMI TCGCGA 2 cut(s) 1470, 3690
CciI TCATGA 2 cut(s) 391, 1650
CfoI GCGC 6 cut(s) 891, 1003, 2680, 3320, 3483, 5474
Cfr10I RCCGGY 1 cut(s) 2231
Cfr13I GGNCC 7 cut(s) 60, 72, 259, 1320, 3559, 3956, 5646
CseI GACGC 5 cut(s) 1316, 3282, 3677, 4392, 4533
CspCI CAANNNNNGTGG 2 cut(s) 661, 696
DraI TTTAAA 1 cut(s) 901
DraIII CACNNNGTG 2 cut(s) 4996, 5488
DrdI GACNNNNNNGTC 1 cut(s) 5582
DseDI GACNNNNNNGTC 1 cut(s) 5582
EaeI YGGCCR 2 cut(s) 693, 4885
Eam1104I CTCTTC 5 cut(s) 42, 581, 2961, 3525, 5519
EarI CTCTTC 5 cut(s) 42, 581, 2961, 3525, 5519
EciI GGCGGA 1 cut(s) 2827
Eco130I CCWWGG 4 cut(s) 2347, 4111, 4519, 4701
Eco147I AGGCCT 1 cut(s) 4743
Eco24I GRGCYC 4 cut(s) 89, 207, 1476, 2257
Eco31I GGTCTC 5 cut(s) 1299, 2494, 3427, 4114, 4196
Eco32I GATATC 1 cut(s) 4452
Eco47I GGWCC 2 cut(s) 259, 3956
Eco47III AGCGCT 3 cut(s) 1002, 2679, 5473
Eco57I CTGAAG 6 cut(s) 728, 1371, 1556, 1853, 3660, 4088
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 1163
EcoNI CCTNNNNNAGG 1 cut(s) 4346
EcoO109I RGGNCCY 1 cut(s) 5646
EcoRI GAATTC 3 cut(s) 1739, 3064, 5675
EcoRII CCWGG 5 cut(s) 42, 2023, 3044, 3952, 5567
EcoRV GATATC 1 cut(s) 4452
EcoT14I CCWWGG 4 cut(s) 2347, 4111, 4519, 4701
EcoT22I ATGCAT 1 cut(s) 3715
EcoT38I GRGCYC 4 cut(s) 89, 207, 1476, 2257
ErhI CCWWGG 4 cut(s) 2347, 4111, 4519, 4701
Esp3I CGTCTC 1 cut(s) 373
FalI AAGNNNNNCTT 8 cut(s) 3639, 3671, 4086, 4118, 4156, 4188, 5256, 5288
FaqI GGGAC 2 cut(s) 1252, 5299
FauNDI CATATG 3 cut(s) 1077, 1150, 3736
FbaI TGATCA 2 cut(s) 2443, 3925
FblI GTMKAC 5 cut(s) 685, 2078, 5031, 5169, 5352
FriOI GRGCYC 4 cut(s) 89, 207, 1476, 2257
FspI TGCGCA 1 cut(s) 890
GlaI GCGC 6 cut(s) 890, 1002, 2679, 3319, 3482, 5473
GsaI CCCAGC 1 cut(s) 5617
GsuI CTGGAG 4 cut(s) 293, 3028, 3798, 4013
HaeII RGCGCY 3 cut(s) 1004, 2681, 5475
HapII CCGG 6 cut(s) 949, 2232, 3315, 3556, 4274, 5145
HgaI GACGC 5 cut(s) 1316, 3282, 3677, 4392, 4533
HhaI GCGC 6 cut(s) 891, 1003, 2680, 3320, 3483, 5474
Hin1I GRCGYC 3 cut(s) 1647, 3669, 4525
Hin6I GCGC 6 cut(s) 889, 1001, 2678, 3318, 3481, 5472
HinP1I GCGC 6 cut(s) 889, 1001, 2678, 3318, 3481, 5472
HindIII AAGCTT 3 cut(s) 1534, 3771, 3947
HpaII CCGG 6 cut(s) 949, 2232, 3315, 3556, 4274, 5145
HphI GGTGA 8 cut(s) 769, 1064, 2363, 4031, 4207, 4777, 5474, 5671
Hpy99I CGWCG 2 cut(s) 3635, 4386
HpyCH4IV ACGT 9 cut(s) 370, 382, 472, 1647, 2363, 3633, 3843, 4086, 4614
HpySE526I ACGT 9 cut(s) 370, 382, 472, 1647, 2363, 3633, 3843, 4086, 4614
Hsp92I GRCGYC 3 cut(s) 1647, 3669, 4525
HspAI GCGC 6 cut(s) 889, 1001, 2678, 3318, 3481, 5472
Kpn2I TCCGGA 2 cut(s) 4273, 5144
Ksp22I TGATCA 2 cut(s) 2443, 3925
LguI GCTCTTC 3 cut(s) 42, 2961, 3525
LmnI GCTCC 7 cut(s) 202, 1551, 2260, 3047, 3397, 3478, 5128
Lsp1109I GCAGC 7 cut(s) 649, 1015, 1793, 3327, 4843, 5073, 5215
LweI GCATC 9 cut(s) 561, 854, 2037, 2874, 2980, 3700, 4334, 4727, 4812
MaeII ACGT 9 cut(s) 370, 382, 472, 1647, 2363, 3633, 3843, 4086, 4614
MaeIII GTNAC 9 cut(s) 64, 775, 1089, 1186, 1663, 3016, 4213, 4505, 5013
MbiI CCGCTC 1 cut(s) 2146
MfeI CAATTG 1 cut(s) 3752
MflI RGATCY 4 cut(s) 1977, 3131, 3781, 5820
MhlI GDGCHC 5 cut(s) 89, 207, 1476, 1944, 2257
MlyI GAGTC 3 cut(s) 1175, 2334, 5773
MmeI TCCRAC 7 cut(s) 2237, 2568, 3097, 3275, 3847, 5630, 5848
Mph1103I ATGCAT 1 cut(s) 3715
MroI TCCGGA 2 cut(s) 4273, 5144
MroXI GAANNNNTTC 8 cut(s) 3068, 3470, 3651, 4101, 4168, 4790, 5003, 5465
MslI CAYNNNNRTG 8 cut(s) 80, 105, 728, 2349, 4040, 4275, 4881, 4905
MspA1I CMGCKG 1 cut(s) 4834
MspI CCGG 6 cut(s) 949, 2232, 3315, 3556, 4274, 5145
MspR9I CCNGG 7 cut(s) 44, 2025, 3046, 3315, 3557, 3954, 5569
MunI CAATTG 1 cut(s) 3752
Mva1269I GAATGC 5 cut(s) 2405, 3623, 4070, 5507, 5771
MvaI CCWGG 5 cut(s) 44, 2025, 3046, 3954, 5569
MvnI CGCG 5 cut(s) 1470, 3690, 3834, 4386, 5741
NciI CCSGG 2 cut(s) 3315, 3557
NdeI CATATG 3 cut(s) 1077, 1150, 3736
NlaIV GGNNCC 8 cut(s) 204, 1953, 2256, 2537, 3043, 3560, 4259, 5648
NmuCI GTSAC 4 cut(s) 775, 1663, 4213, 4505
NruI TCGCGA 2 cut(s) 1470, 3690
NsbI TGCGCA 1 cut(s) 890
NsiI ATGCAT 1 cut(s) 3715
NspI RCATGY 7 cut(s) 1596, 1763, 2752, 3313, 3442, 3791, 4414
NspV TTCGAA 3 cut(s) 1308, 2557, 4461
OliI CACNNNNGTG 1 cut(s) 2349
PaeI GCATGC 1 cut(s) 1596
PagI TCATGA 2 cut(s) 391, 1650
PceI AGGCCT 1 cut(s) 4743
PciI ACATGT 2 cut(s) 1759, 2748
PciSI GCTCTTC 3 cut(s) 42, 2961, 3525
PcsI WCGNNNNNNNCGW 3 cut(s) 283, 676, 2802
PctI GAATGC 5 cut(s) 2405, 3623, 4070, 5507, 5771
PdmI GAANNNNTTC 8 cut(s) 3068, 3470, 3651, 4101, 4168, 4790, 5003, 5465
PflFI GACNNNGTC 1 cut(s) 5579
PflMI CCANNNNNTGG 3 cut(s) 1151, 1603, 3968
PfoI TCCNGGA 2 cut(s) 3044, 5567
PleI GAGTC 3 cut(s) 1174, 2333, 5773
PpsI GAGTC 3 cut(s) 1174, 2333, 5773
Ppu21I YACGTR 2 cut(s) 371, 473
PscI ACATGT 2 cut(s) 1759, 2748
PshBI ATTAAT 2 cut(s) 3381, 5078
PsiI TTATAA 1 cut(s) 1208
Psp1406I AACGTT 1 cut(s) 4086
Psp6I CCWGG 5 cut(s) 42, 2023, 3044, 3952, 5567
PspFI CCCAGC 1 cut(s) 5613
PspGI CCWGG 5 cut(s) 42, 2023, 3044, 3952, 5567
PspN4I GGNNCC 8 cut(s) 204, 1953, 2256, 2537, 3043, 3560, 4259, 5648
PspPI GGNCC 7 cut(s) 60, 72, 259, 1320, 3559, 3956, 5646
PstI CTGCAG 1 cut(s) 4689
PsuI RGATCY 4 cut(s) 1977, 3131, 3781, 5820
PsyI GACNNNGTC 1 cut(s) 5579
PvuII CAGCTG 1 cut(s) 4834
RruI TCGCGA 2 cut(s) 1470, 3690
RseI CAYNNNNRTG 8 cut(s) 80, 105, 728, 2349, 4040, 4275, 4881, 4905
SapI GCTCTTC 3 cut(s) 42, 2961, 3525
Sau96I GGNCC 7 cut(s) 60, 72, 259, 1320, 3559, 3956, 5646
ScaI AGTACT 3 cut(s) 749, 2100, 5688
SchI GAGTC 3 cut(s) 1175, 2334, 5773
ScrFI CCNGG 7 cut(s) 44, 2025, 3046, 3315, 3557, 3954, 5569
SduI GDGCHC 5 cut(s) 89, 207, 1476, 1944, 2257
SfaNI GCATC 9 cut(s) 561, 854, 2037, 2874, 2980, 3700, 4334, 4727, 4812
SfcI CTRYAG 5 cut(s) 1404, 1794, 3598, 4685, 4969
SfuI TTCGAA 3 cut(s) 1308, 2557, 4461
SinI GGWCC 2 cut(s) 259, 3956
SmiMI CAYNNNNRTG 8 cut(s) 80, 105, 728, 2349, 4040, 4275, 4881, 4905
SmlI CTYRAG 1 cut(s) 2885
SmoI CTYRAG 1 cut(s) 2885
SphI GCATGC 1 cut(s) 1596
SseBI AGGCCT 1 cut(s) 4743
SspI AATATT 2 cut(s) 592, 676
StuI AGGCCT 1 cut(s) 4743
StyD4I CCNGG 7 cut(s) 42, 2023, 3044, 3313, 3555, 3952, 5567
StyI CCWWGG 4 cut(s) 2347, 4111, 4519, 4701
TaiI ACGT 9 cut(s) 373, 385, 475, 1650, 2366, 3636, 3846, 4089, 4617
TauI GCSGC 3 cut(s) 55, 228, 656
TseFI GTSAC 4 cut(s) 775, 1663, 4213, 4505
TseI GCWGC 7 cut(s) 662, 1028, 1781, 3340, 4831, 5061, 5203
Tsp45I GTSAC 4 cut(s) 775, 1663, 4213, 4505
Tth111I GACNNNGTC 1 cut(s) 5579
Van91I CCANNNNNTGG 3 cut(s) 1151, 1603, 3968
VpaK11BI GGWCC 2 cut(s) 259, 3956
VspI ATTAAT 2 cut(s) 3381, 5078
XagI CCTNNNNNAGG 1 cut(s) 4346
XbaI TCTAGA 2 cut(s) 1330, 1823
XceI RCATGY 7 cut(s) 1596, 1763, 2752, 3313, 3442, 3791, 4414
XcmI CCANNNNNNNNNTGG 1 cut(s) 1732
XmiI GTMKAC 5 cut(s) 685, 2078, 5031, 5169, 5352
XmnI GAANNNNTTC 8 cut(s) 3068, 3470, 3651, 4101, 4168, 4790, 5003, 5465
ZraI GACGTC 1 cut(s) 1648
ZrmI AGTACT 3 cut(s) 749, 2100, 5688
Zsp2I ATGCAT 1 cut(s) 3715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.