Rh5DG199100

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016003
Physical Location & Seq
Forward (+)
22157050 .. 22173914
16865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG199100.1

Sequence Viewer

Length: 5475 bp
ATGTCTGAGATTGTGGTTGCAGAGCCCATCTATGATGATCGAGCAGAGGAAAAAAGACCCATTACCAGGTATCTCACCATGGGCAGTGGGAGTGATCATAATCATCCTCCTGAGCCATTTGAGAGCGAGAGATTGCCTGCCACTTTGGCCTCAAAAATCCGGAGCTTTCTTCGGGTTGCAAACTTGATCGAAAGCGAGGAGCCTCGGATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCGTTCGAATGGGAGGGGTGTTCGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACAATTAGGAAACGGAAGGAAAAGAGTGATCTACGCGAGTTAAGACGCGTTTATCATTCTTACAAGGAGTACATCATCAAACATGAAGGAGCATTTAGCATAGAAAATAGTCACAGAGAGAAGTTGATAAATGCGCGTATAATTGGTTCTGTATTGTTTGAAGTCTTAAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTGGCTAATAGAGGTGGTATCCAGACAAAACCTTATGATATATATAATATTCTACCACTTGATCAAGGAGGTATTCAGCAACCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAATACGGTATATTCGTGGCATACCTTCCAATGAAGATCTTCAGAAACATGGGACTTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTCTACTCCTTGCTAATATTCACAAACGGAAAACTCATAAACAGACATCTGTCTCAAAATTAGGAGATGCTGCAGTGGACGAGTTAATGCGGAGAATTTTTAAAAATTATACAAACTGGTGCAAGTTTTTGGGAAGGAAAAGCAACATCCGGTTACCTTATGTGAAACAAGAAGCCCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGCGAGGCTGCAAACTTGCGATTCATGCCAGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAGGTCATGCCAGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTTGTTGCCCCCATATATGGGGTTATAAGAGAGGAAGCCGAGAAAAGCAAGTGTGGGACAGCTGACCATTCCACATGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTTCTGATTGTTTTGAAATCGGTTGGCCCATGCGTCTAGATCACGATTTCTTCTTTACAGACTCTCCAACAAAATCCAATGCTAAGAATGCTTCAGCATCCACTGCTCCTGTAGAAGAAAGAAGGAAGGAAGATGAGGAGGAAGATGAAGTTGGGGTTAGTAAAGAAGAAGTCCGTGAACCCAAATGGTTGGGCAAAACAAATTTCGTAGAAGTACGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCACTTCAGGCATTAATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAGTTGTTTGACAAAGTTATATTTGAGGATATCATGAGCATCTTCATTACATCTGCATTTCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACACTGGACTTCTCTGCAAAAGTAAAACATGTTATGAAGCTCGGTGTTGCAATGATATGGACTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATCCCACAACATACGGAAGCTGGGTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATTTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTATTCGCAAATACATTGAGGTCTCAAATTACCAAATATGCACCATTCTGTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGATTGCAAGAAAGCCAGCTTTCAGTGTTGAAGTATACATTGTTTTGGGTACTTGTACTATTGAGCAAATTTTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCCAATGTATGATTGGCATGAGCTTTTTCCGAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGAACTTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCTGCATTCAATATTTCTCTTATTCCACCCTCATTGAGGAATGACCAAAAGAGAAGAAAAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAGACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTCAGGACTTTAGGATGATTAAAGTTCCAGATCTTTTGGCTAAATGTATCGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCGTGGCAAAGTTGTGAAAGTTCTCCAAGATATATTTGAGCTTGTAATCAATGATATGATGACGACTGGCTTCAGGATATTAGAGTTGCTGGATTCATCCCAACAGACTGAAACAGATGCTGCTTATTTTTCTGGAAGCATTGAATCCCCATTATTTGGATCTGCTGGTGGCAAGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTGAATGAGCAGATCAAGCGTTTTCTTCTGTTGCTTACTGTCCAAGATACAGCAATGGACATTCCAACTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAATATGCCACATGCACCCACAGTGTCCAATATGGTGCCATTCAGTGTTATGACACCACATTACCTGGAGGATATTAATTTTTCAATGGAGGAGCTGCATTCAAGCCAGCAAGAAGTTTCTATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCCTGGAGCGCATGGGTTATGAAAATTTGGATGGGTTGGAGAAGGACAAAAAGGAAGAGCTTAGAAATTGGGCTTCTTTCCGGGGCCAAACACTGAGCAGAACAGTTAGAGGAATGATGTACTATAGAGAAGCCTTAAAACTGCAAGCATTTCTTGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATGCATTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACTTATGTTGTTACCTGCCAATTATTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAAAAGATACCCAACTCTCCGTGTTGCTTATGTTGAGGAGAAAGAAGAGATAGTGGAGAACAAGCCTTGCAAAGTTTATTCTTCAGTATTGGTTAAAGCTATCCCCAAGTATGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTGCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAACCATGGAATAATCTTCACTCGTGGTGAAGCTCTCCAAACCATTGATATGAACCAAGACAGTTATTTGGAGGAAGCCTTCAAAATGAGAAATCTTCTGCAAGAATTTCTTCAGAACCGAGGACGGCGACCTCCTATACTGCTTGGTTTGAGGGAACACATATTTACAGGAAGTGTTTCTTCTCTTGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTACGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGATTAAGCAAAGCATCAAAAACAATTAACCTGAGCGAGGATGTTTTTGCTGGGTATAATTCTACCTTACGACGGGGATGGATCACGTATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAGTTTGAAGCTAAGGTTGCTAATGGTAACAGTGAACAAACTTTAAGCCGTGACATATACCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTACGGACAGCTATACCTAGTTCTCAGTGGCTTGGAAAAAGCTCTCATAATTGAGGCTAGACTGCAGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGCTATTAACAGGAATGCCAATGGTGATGGAAATTGGACTTGAGAAAGGGTTCCTCAATGCACTAAAAGATTTTGTCCTGATGCAATTGCAGCTGGCTTCTGTTTTTTTCACGTTCTCATTTGGAACAAAAATCCATTATTACGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTTGTTGTGTTTCATACTAGCTTCACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTCGTCAAAGGATTTGAGTTACTGCTCCTGTTGATTGTTTATAACTTGTTTAGACGGTCCTATGAGAGCGACATGGCGTATGTATTAGTCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTCGCTCCATTTCTTTTTAATCCGTCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGATTGGAATAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCACCAAGATAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGAGTTCTAGGTTGTTTGAAATACTCCTTTCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCAGGAATTTTCTGGTTTATTTGCTTTCATGGATAGTGATCCTCGTTGTTTTCTTGTTAGTAAAGGTAAACCTTACCATCGCTTCTATTAGAAACTATGCTCATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1824

Amino Acids

211.51

Weight (kDa)

8.21

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 112 1.7e-07 Vta1 like
FKS1_dom1 PF14288 326 - 437 1.5e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 879 - 1072 2.9e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1079 - 1164 4.6e-25 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1169 - 1764 1.4e-195 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1199, 5022
AatII GACGTC 1 cut(s) 4516
Acc36I ACCTGC 1 cut(s) 3740
AccB1I GGYRCC 1 cut(s) 3328
AccB7I CCANNNNNTGG 4 cut(s) 1142, 1594, 3119, 3956
AccI GTMKAC 2 cut(s) 2069, 5340
AccII CGCG 4 cut(s) 369, 381, 469, 2032
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 4 cut(s) 223, 644, 880, 4504
AclWI GGATC 6 cut(s) 2814, 3130, 3754, 4388, 5192, 5398
AcoI YGGCCR 2 cut(s) 2633, 4873
AcyI GRCGYC 1 cut(s) 4513
AflIII ACRYGT 3 cut(s) 379, 1750, 2739
AjnI CCWGG 5 cut(s) 65, 534, 3357, 3462, 3940
AjuI GAANNNNNNNTTGG 2 cut(s) 1419, 1451
AleI CACNNNNGTG 1 cut(s) 2340
AloI GAACNNNNNNTCC 4 cut(s) 1842, 1874, 4670, 4702
Alw21I GWGCWC 1 cut(s) 3949
Alw26I GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3759, 4184
Alw44I GTGCAC 1 cut(s) 3945
AlwI GGATC 6 cut(s) 2814, 3130, 3754, 4388, 5192, 5398
AlwNI CAGNNNCTG 2 cut(s) 535, 3083
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 6 cut(s) 147, 531, 1310, 2633, 3544, 4873
ApaLI GTGCAC 1 cut(s) 3945
AseI ATTAAT 2 cut(s) 1571, 3369
Asp700I GAANNNNTTC 6 cut(s) 693, 3458, 3636, 4089, 4156, 4778
AspA2I CCTAGG 1 cut(s) 2015
AspLEI GCGC 3 cut(s) 469, 2032, 3471
AspS9I GGNCC 9 cut(s) 256, 524, 532, 1311, 1942, 3544, 3781, 5037, 5254
AsuC2I CCSGG 1 cut(s) 3542
AsuHPI GGTGA 9 cut(s) 67, 1055, 2327, 2354, 3923, 4019, 4195, 4765, 5204
AsuII TTCGAA 2 cut(s) 266, 2350
AvaII GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
AvrII CCTAGG 1 cut(s) 2015
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 3949
BalI TGGCCA 1 cut(s) 2635
BanI GGYRCC 1 cut(s) 3328
BanII GRGCYC 2 cut(s) 27, 2248
BarI GAAGNNNNNNTAC 4 cut(s) 1810, 1842, 2747, 2779
BauI CACGAG 1 cut(s) 4002
BbsI GAAGAC 5 cut(s) 1278, 2595, 2725, 2864, 2964
Bbv12I GWGCWC 1 cut(s) 3949
BccI CCATC 8 cut(s) 35, 1921, 2970, 3485, 4371, 4750, 5265, 5450
BceAI ACGGC 3 cut(s) 4121, 4476, 4888
BciT130I CCWGG 5 cut(s) 67, 536, 3359, 3464, 3942
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 3 cut(s) 94, 595, 3913
BcnI CCSGG 1 cut(s) 3542
BcoDI GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3759, 4184
BfaI CTAG 8 cut(s) 1322, 1815, 2016, 3692, 4628, 4668, 4941, 5283
BfmI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3583, 4673, 4957
BfuAI ACCTGC 1 cut(s) 3740
BfuI GTATCC 1 cut(s) 563
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 3 cut(s) 691, 2905, 4437
BlnI CCTAGG 1 cut(s) 2015
BlpI GCTNAGC 1 cut(s) 3687
BmcAI AGTACT 1 cut(s) 740
Bme1390I CCNGG 6 cut(s) 67, 536, 3359, 3464, 3542, 3942
Bme18I GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
BmgT120I GGNCC 9 cut(s) 256, 524, 532, 1311, 1942, 3544, 3781, 5037, 5254
BmiI GGNNCC 7 cut(s) 201, 1944, 2247, 2528, 3330, 3545, 4781
BmrFI CCNGG 6 cut(s) 67, 536, 3359, 3464, 3542, 3942
BmrI ACTGGG 2 cut(s) 4705, 4923
BmuI ACTGGG 2 cut(s) 4705, 4923
BoxI GACNNNNGTC 1 cut(s) 839
BpiI GAAGAC 5 cut(s) 1278, 2595, 2725, 2864, 2964
BpmI CTGGAG 3 cut(s) 3380, 3485, 3783
Bpu10I CCTNAGC 3 cut(s) 111, 4331, 4455
Bpu1102I GCTNAGC 1 cut(s) 3687
Bpu14I TTCGAA 2 cut(s) 266, 2350
BpuEI CTTGAG 1 cut(s) 4790
BpuMI CCSGG 1 cut(s) 3542
BsaAI YACGTR 1 cut(s) 4386
BsaBI GATNNNNATC 2 cut(s) 99, 5402
BsaHI GRCGYC 1 cut(s) 4513
BsaI GGTCTC 3 cut(s) 1975, 3759, 4184
BsaWI WCCGGW 2 cut(s) 159, 939
BsaXI ACNNNNNCTCC 2 cut(s) 1333, 1363
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 8 cut(s) 911, 1102, 1731, 2636, 3034, 4711, 4918, 5168
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 1695, 1779, 3234
Bse8I GATNNNNATC 2 cut(s) 99, 5402
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 5 cut(s) 67, 536, 3359, 3464, 3942
BseJI GATNNNNATC 2 cut(s) 99, 5402
BseMI GCAATG 3 cut(s) 1695, 1779, 3234
BseNI ACTGG 8 cut(s) 911, 1102, 1731, 2636, 3034, 4711, 4918, 5168
BseRI GAGGAG 5 cut(s) 212, 1436, 2383, 3398, 3848
BseSI GKGCMC 1 cut(s) 3949
BseX3I CGGCCG 1 cut(s) 4873
BseYI CCCAGC 2 cut(s) 1854, 4349
Bsh1236I CGCG 4 cut(s) 369, 381, 469, 2032
Bsh1285I CGRYCG 1 cut(s) 4876
BshFI GGCC 6 cut(s) 149, 533, 1312, 2635, 3546, 4875
BshNI GGYRCC 1 cut(s) 3328
BsiEI CGRYCG 1 cut(s) 4876
BsiHKAI GWGCWC 1 cut(s) 3949
BsiSI CCGG 5 cut(s) 160, 522, 940, 2619, 3541
BslFI GGGAC 4 cut(s) 721, 1243, 1928, 5159
BsmAI GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3759, 4184
BsmBI CGTCTC 1 cut(s) 3253
BsmFI GGGAC 4 cut(s) 721, 1243, 1928, 5159
BsmI GAATGC 4 cut(s) 1378, 2396, 3391, 4749
BsnI GGCC 6 cut(s) 149, 533, 1312, 2635, 3546, 4875
Bso31I GGTCTC 3 cut(s) 1975, 3759, 4184
Bsp119I TTCGAA 2 cut(s) 266, 2350
Bsp1286I GDGCHC 3 cut(s) 27, 2248, 3949
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3687
Bsp19I CCATGG 2 cut(s) 78, 3985
BspACI CCGC 4 cut(s) 223, 644, 880, 4504
BspANI GGCC 6 cut(s) 149, 533, 1312, 2635, 3546, 4875
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 4 cut(s) 369, 381, 469, 2032
BspHI TCATGA 3 cut(s) 1641, 2160, 4390
BspLI GGNNCC 7 cut(s) 201, 1944, 2247, 2528, 3330, 3545, 4781
BspMAI CTGCAG 3 cut(s) 309, 865, 4677
BspMI ACCTGC 1 cut(s) 3740
BspPI GGATC 6 cut(s) 2814, 3130, 3754, 4388, 5192, 5398
BspQI GCTCTTC 1 cut(s) 3510
BspT104I TTCGAA 2 cut(s) 266, 2350
BspT107I GGYRCC 1 cut(s) 3328
BspTNI GGTCTC 3 cut(s) 1975, 3759, 4184
BsrDI GCAATG 3 cut(s) 1695, 1779, 3234
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 8 cut(s) 911, 1102, 1731, 2636, 3034, 4711, 4918, 5168
BssAI RCCGGY 1 cut(s) 521
BssNAI GTATAC 1 cut(s) 2070
BssNI GRCGYC 1 cut(s) 4513
BssSI CACGAG 1 cut(s) 4002
BssT1I CCWWGG 6 cut(s) 78, 2015, 2338, 3985, 4507, 4689
Bst1107I GTATAC 1 cut(s) 2070
Bst2BI CACGAG 1 cut(s) 4002
Bst2UI CCWGG 5 cut(s) 67, 536, 3359, 3464, 3942
Bst6I CTCTTC 3 cut(s) 1882, 3510, 3837
BstACI GRCGYC 1 cut(s) 4513
BstAPI GCANNNNNTGC 2 cut(s) 1388, 3683
BstBAI YACGTR 1 cut(s) 4386
BstBI TTCGAA 2 cut(s) 266, 2350
BstC8I GCNNGC 7 cut(s) 138, 1137, 1585, 2051, 3401, 3606, 4824
BstDSI CCRYGG 2 cut(s) 78, 3985
BstEII GGTNACC 1 cut(s) 942
BstENI CCTNNNNNAGG 2 cut(s) 2425, 4334
BstFNI CGCG 4 cut(s) 369, 381, 469, 2032
BstHHI GCGC 3 cut(s) 469, 2032, 3471
BstMAI GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3759, 4184
BstMCI CGRYCG 1 cut(s) 4876
BstNI CCWGG 5 cut(s) 67, 536, 3359, 3464, 3942
BstNSI RCATGY 8 cut(s) 1093, 1587, 1754, 2743, 3308, 3430, 3776, 4402
BstPAI GACNNNNGTC 1 cut(s) 839
BstPI GGTNACC 1 cut(s) 942
BstSCI CCNGG 6 cut(s) 65, 534, 3357, 3462, 3540, 3940
BstSFI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3583, 4673, 4957
BstSLI GKGCMC 1 cut(s) 3949
BstUI CGCG 4 cut(s) 369, 381, 469, 2032
BstV2I GAAGAC 5 cut(s) 1278, 2595, 2725, 2864, 2964
BstX2I RGATCY 5 cut(s) 691, 2806, 2905, 3122, 4437
BstXI CCANNNNNNTGG 4 cut(s) 1474, 3450, 3743, 3986
BstYI RGATCY 5 cut(s) 691, 2806, 2905, 3122, 4437
BstZ17I GTATAC 1 cut(s) 2070
BstZI CGGCCG 1 cut(s) 4873
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 6 cut(s) 149, 533, 1312, 2635, 3546, 4875
BtgI CCRYGG 2 cut(s) 78, 3985
BtgZI GCGATG 1 cut(s) 5428
BtsI GCAGTG 3 cut(s) 91, 870, 1386
BveI ACCTGC 1 cut(s) 3740
Cac8I GCNNGC 7 cut(s) 138, 1137, 1585, 2051, 3401, 3606, 4824
CaiI CAGNNNCTG 2 cut(s) 535, 3083
CciI TCATGA 3 cut(s) 1641, 2160, 4390
CfoI GCGC 3 cut(s) 469, 2032, 3471
Cfr10I RCCGGY 1 cut(s) 521
Cfr13I GGNCC 9 cut(s) 256, 524, 532, 1311, 1942, 3544, 3781, 5037, 5254
CseI GACGC 3 cut(s) 387, 1307, 3270
CsiI ACCWGGT 1 cut(s) 65
CspCI CAANNNNNGTGG 4 cut(s) 1593, 1628, 2406, 2441
DraI TTTAAA 1 cut(s) 892
EaeI YGGCCR 2 cut(s) 2633, 4873
EagI CGGCCG 1 cut(s) 4873
Eam1104I CTCTTC 3 cut(s) 1882, 3510, 3837
EarI CTCTTC 3 cut(s) 1882, 3510, 3837
EclXI CGGCCG 1 cut(s) 4873
Eco130I CCWWGG 6 cut(s) 78, 2015, 2338, 3985, 4507, 4689
Eco24I GRGCYC 2 cut(s) 27, 2248
Eco31I GGTCTC 3 cut(s) 1975, 3759, 4184
Eco32I GATATC 2 cut(s) 1639, 4584
Eco47I GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
Eco52I CGGCCG 1 cut(s) 4873
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 942
EcoNI CCTNNNNNAGG 2 cut(s) 2425, 4334
EcoO109I RGGNCCY 2 cut(s) 532, 3781
EcoO65I GGTNACC 1 cut(s) 942
EcoRI GAATTC 1 cut(s) 3139
EcoRII CCWGG 5 cut(s) 65, 534, 3357, 3462, 3940
EcoRV GATATC 2 cut(s) 1639, 4584
EcoT14I CCWWGG 6 cut(s) 78, 2015, 2338, 3985, 4507, 4689
EcoT22I ATGCAT 2 cut(s) 3679, 3700
EcoT38I GRGCYC 2 cut(s) 27, 2248
ErhI CCWWGG 6 cut(s) 78, 2015, 2338, 3985, 4507, 4689
Esp3I CGTCTC 1 cut(s) 3253
FaqI GGGAC 4 cut(s) 721, 1243, 1928, 5159
FauNDI CATATG 3 cut(s) 1068, 1075, 1141
FbaI TGATCA 3 cut(s) 94, 595, 3913
FblI GTMKAC 2 cut(s) 2069, 5340
FriOI GRGCYC 2 cut(s) 27, 2248
FspBI CTAG 8 cut(s) 1322, 1815, 2016, 3692, 4628, 4668, 4941, 5283
GlaI GCGC 3 cut(s) 468, 2031, 3470
GsaI CCCAGC 2 cut(s) 1858, 4353
GsuI CTGGAG 3 cut(s) 3380, 3485, 3783
HaeIII GGCC 6 cut(s) 149, 533, 1312, 2635, 3546, 4875
HapII CCGG 5 cut(s) 160, 522, 940, 2619, 3541
HgaI GACGC 3 cut(s) 387, 1307, 3270
HhaI GCGC 3 cut(s) 469, 2032, 3471
Hin1I GRCGYC 1 cut(s) 4513
Hin6I GCGC 3 cut(s) 467, 2030, 3469
HinP1I GCGC 3 cut(s) 467, 2030, 3469
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 1525, 3935
HpaI GTTAAC 1 cut(s) 1095
HpaII CCGG 5 cut(s) 160, 522, 940, 2619, 3541
HphI GGTGA 9 cut(s) 67, 1055, 2327, 2354, 3923, 4019, 4195, 4765, 5204
Hpy99I CGWCG 2 cut(s) 264, 4374
HpyCH4IV ACGT 4 cut(s) 1501, 4385, 4513, 4841
HpySE526I ACGT 4 cut(s) 1501, 4385, 4513, 4841
Hsp92I GRCGYC 1 cut(s) 4513
HspAI GCGC 3 cut(s) 467, 2030, 3469
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 3 cut(s) 94, 595, 3913
KspAI GTTAAC 1 cut(s) 1095
LguI GCTCTTC 1 cut(s) 3510
MabI ACCWGGT 1 cut(s) 65
MaeI CTAG 8 cut(s) 1322, 1815, 2016, 3692, 4628, 4668, 4941, 5283
MaeII ACGT 4 cut(s) 1501, 4385, 4513, 4841
MaeIII GTNAC 8 cut(s) 443, 942, 3727, 4201, 4469, 4493, 4998, 5068
MfeI CAATTG 1 cut(s) 4814
MflI RGATCY 5 cut(s) 691, 2806, 2905, 3122, 4437
MhlI GDGCHC 3 cut(s) 27, 2248, 3949
MlsI TGGCCA 1 cut(s) 2635
MluI ACGCGT 1 cut(s) 379
MluNI TGGCCA 1 cut(s) 2635
MlyI GAGTC 3 cut(s) 1166, 1340, 2325
MmeI TCCRAC 3 cut(s) 1376, 3263, 3477
Mox20I TGGCCA 1 cut(s) 2635
Mph1103I ATGCAT 2 cut(s) 3679, 3700
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 6 cut(s) 693, 3458, 3636, 4089, 4156, 4778
MscI TGGCCA 1 cut(s) 2635
MslI CAYNNNNRTG 4 cut(s) 2340, 4028, 4263, 4893
Msp20I TGGCCA 1 cut(s) 2635
MspA1I CMGCKG 2 cut(s) 1235, 4822
MspI CCGG 5 cut(s) 160, 522, 940, 2619, 3541
MspR9I CCNGG 6 cut(s) 67, 536, 3359, 3464, 3542, 3942
MunI CAATTG 1 cut(s) 4814
Mva1269I GAATGC 4 cut(s) 1378, 2396, 3391, 4749
MvaI CCWGG 5 cut(s) 67, 536, 3359, 3464, 3942
MvnI CGCG 4 cut(s) 369, 381, 469, 2032
NciI CCSGG 1 cut(s) 3542
NcoI CCATGG 2 cut(s) 78, 3985
NdeI CATATG 3 cut(s) 1068, 1075, 1141
NlaIV GGNNCC 7 cut(s) 201, 1944, 2247, 2528, 3330, 3545, 4781
NmeAIII GCCGAG 1 cut(s) 1237
NmuCI GTSAC 4 cut(s) 443, 4201, 4493, 5068
NsiI ATGCAT 2 cut(s) 3679, 3700
NspI RCATGY 8 cut(s) 1093, 1587, 1754, 2743, 3308, 3430, 3776, 4402
NspV TTCGAA 2 cut(s) 266, 2350
OliI CACNNNNGTG 1 cut(s) 2340
PaeI GCATGC 1 cut(s) 1587
PagI TCATGA 3 cut(s) 1641, 2160, 4390
PciI ACATGT 2 cut(s) 1750, 2739
PciSI GCTCTTC 1 cut(s) 3510
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PctI GAATGC 4 cut(s) 1378, 2396, 3391, 4749
PdmI GAANNNNTTC 6 cut(s) 693, 3458, 3636, 4089, 4156, 4778
PfeI GAWTC 9 cut(s) 1032, 1598, 2347, 2768, 3056, 3107, 4245, 4596, 5136
PflMI CCANNNNNTGG 4 cut(s) 1142, 1594, 3119, 3956
PfoI TCCNGGA 1 cut(s) 3462
PleI GAGTC 3 cut(s) 1165, 1340, 2324
PpsI GAGTC 3 cut(s) 1165, 1340, 2324
Ppu21I YACGTR 1 cut(s) 4386
PpuMI RGGWCCY 1 cut(s) 3781
PscI ACATGT 2 cut(s) 1750, 2739
PshAI GACNNNNGTC 1 cut(s) 839
PshBI ATTAAT 2 cut(s) 1571, 3369
PsiI TTATAA 2 cut(s) 1199, 5022
Psp5II RGGWCCY 1 cut(s) 3781
Psp6I CCWGG 5 cut(s) 65, 534, 3357, 3462, 3940
PspEI GGTNACC 1 cut(s) 942
PspFI CCCAGC 2 cut(s) 1854, 4349
PspGI CCWGG 5 cut(s) 65, 534, 3357, 3462, 3940
PspN4I GGNNCC 7 cut(s) 201, 1944, 2247, 2528, 3330, 3545, 4781
PspPI GGNCC 9 cut(s) 256, 524, 532, 1311, 1942, 3544, 3781, 5037, 5254
PspPPI RGGWCCY 1 cut(s) 3781
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 3 cut(s) 309, 865, 4677
PstNI CAGNNNCTG 2 cut(s) 535, 3083
PsuI RGATCY 5 cut(s) 691, 2806, 2905, 3122, 4437
PvuII CAGCTG 2 cut(s) 1235, 4822
RseI CAYNNNNRTG 4 cut(s) 2340, 4028, 4263, 4893
SapI GCTCTTC 1 cut(s) 3510
Sau96I GGNCC 9 cut(s) 256, 524, 532, 1311, 1942, 3544, 3781, 5037, 5254
ScaI AGTACT 1 cut(s) 740
SchI GAGTC 3 cut(s) 1166, 1340, 2325
ScrFI CCNGG 6 cut(s) 67, 536, 3359, 3464, 3542, 3942
SduI GDGCHC 3 cut(s) 27, 2248, 3949
SexAI ACCWGGT 1 cut(s) 65
SfcI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3583, 4673, 4957
SfuI TTCGAA 2 cut(s) 266, 2350
SinI GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
SmiMI CAYNNNNRTG 4 cut(s) 2340, 4028, 4263, 4893
SmlI CTYRAG 1 cut(s) 4769
SmoI CTYRAG 1 cut(s) 4769
SphI GCATGC 1 cut(s) 1587
SsiI CCGC 4 cut(s) 223, 644, 880, 4504
SspI AATATT 3 cut(s) 583, 808, 2404
SspMI CTAG 8 cut(s) 1322, 1815, 2016, 3692, 4628, 4668, 4941, 5283
StyD4I CCNGG 6 cut(s) 65, 534, 3357, 3462, 3540, 3940
StyI CCWWGG 6 cut(s) 78, 2015, 2338, 3985, 4507, 4689
TaiI ACGT 4 cut(s) 1504, 4388, 4516, 4844
TatI WGTACW 8 cut(s) 402, 738, 2089, 2262, 2736, 2742, 2778, 3579
TauI GCSGC 2 cut(s) 225, 647
TfiI GAWTC 9 cut(s) 1032, 1598, 2347, 2768, 3056, 3107, 4245, 4596, 5136
TseFI GTSAC 4 cut(s) 443, 4201, 4493, 5068
Tsp45I GTSAC 4 cut(s) 443, 4201, 4493, 5068
TspGWI ACGGA 7 cut(s) 361, 832, 1448, 1863, 3806, 4629, 5118
Van91I CCANNNNNTGG 4 cut(s) 1142, 1594, 3119, 3956
VneI GTGCAC 1 cut(s) 3945
VpaK11BI GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
VspI ATTAAT 2 cut(s) 1571, 3369
XagI CCTNNNNNAGG 2 cut(s) 2425, 4334
XbaI TCTAGA 2 cut(s) 1321, 1814
XceI RCATGY 8 cut(s) 1093, 1587, 1754, 2743, 3308, 3430, 3776, 4402
XcmI CCANNNNNNNNNTGG 2 cut(s) 2184, 2515
XmaJI CCTAGG 1 cut(s) 2015
XmiI GTMKAC 2 cut(s) 2069, 5340
XmnI GAANNNNTTC 6 cut(s) 693, 3458, 3636, 4089, 4156, 4778
XspI CTAG 8 cut(s) 1322, 1815, 2016, 3692, 4628, 4668, 4941, 5283
ZraI GACGTC 1 cut(s) 4514
ZrmI AGTACT 1 cut(s) 740
Zsp2I ATGCAT 2 cut(s) 3679, 3700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.