Rw5G018140

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016003
Physical Location & Seq
Forward (+)
23438898 .. 23455269
16372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018140.1

Sequence Viewer

Length: 5793 bp
ATGTCTGAGATTGTGGTTGCAGAGCCCATCTATGATGATCGAGCAGAGGAAAAAAGACCCATTACAAGGTATCTCACCATGGGCAGTGGGAGTGATCATAATCATCCTCCTGAGCCATTTGAGAGCGAGAGATTGCCTTCCACTTTGGCCTCAAAAATCCGGAGCTTTCTTCGGGTTGCAAACTTGATCGAAAGCGAGGAGCCTCGGATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCGTTCGAATGGGAGGGGTGTTCGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACAATTAGGAAACGGAAGGAAAAGAGTGATATACGCGAGTTAAGACGCATTTATCATGCTTACAAGGAGTACATCATCAAACATGAAGGAGCATTTAGCATAGAAAATAGTCACAGAGAGAAGTTGATAAATGCGCGTATAATTGGTTCTGTATTGTTTGAAGTCTTAAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTGGCTAATAGAGGTGGTATCCAGACAAAACCTTATGATATATATAATATTCTACCACTTGATCAAGGAGGTATTCAGCAACCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAATACGGTATATTCGTGGCATACCTTCCAATGAAGATCTTCAGAAACATGGGACTTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTCTACTCCTTGCTAATATTCACAAACGGAAAACTCATAAACAGACATCTGTCTCAAAATTAGGAGATGCTGCAGTGGACGAGTTAATGCGGAGAATTTTTAAAAATTATACAAACTGGTGCAAGTTTTTGGGAAGGAAAAGCAACATCCGGTTACCTTATGTGAAACAAGAAGCCCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGCGAGGCTGCAAACTTGCGATTCATGCCAGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAGGTCATGCCAGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTTGTTGCCCCCATATATGGGGTTATAAGAGAGGAAGCCGAGAAAAGCAAGTGTGGGACAGCTGACCATTCCACATGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTTCTGATTGTTTTGAAATCGGTTGGCCCATGCGTCTAGATCACGATTTCTTCTTTACAGACTCTCCAACAAAATCCAATGCTAAGAATGCTTCAGCATCCACTGCTCCTGTAGAAGAAAGAAGGAAGGAAGATGAGGAGGAAGATGAAGTTGGGGTTACTAAAGAAGAAGTCCGTGAACCCAAATGGTTGGGCAAAACAAATTTCGTAGAAGTACGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCACTTCAGGCATTAATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAGTTGTTTGACAAAGTTATATTTGAGGATATCATGAGCATCTTCATTACATCTGCATTTCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACACTGGACTTCTCTGCAAAAGTAAAACATGTTATGAAGCTCGGTGTTGCAATGATATGGACTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATCCCACAACATACGGAAACTGGGTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATTTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTATTCGCAAATACATTGAGGTCTCAAATTACCGAATATGCACCATTCTGTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGATTGCAAGAAAGCCAGCTTTCAGTGTTGAAGTATACATTGTTTTGGGTACTTGTACTATTGAGCAAATTTTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCCAATGTATGATTGGCATGAGCTTTTTCCGAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGAACTTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATTGAGGAATGACCAAAAGAGAAGAAAAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAGACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCGTACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTGAGAACTTTAGGATGATTAAAGTTCCAGATCTTTTGGCTAAATGTATCGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCGTGGCAAAGTTGTGAAAGTTCTCCAAGATATATTTGAGCTTGTAATCAATGATATGATGACGACTGGCTTCAGGATATTAGAGTTGCTGGATTCATCCCAACAGACTGAAACAGATGCTGCTTATTTTTCTGGAAGCATTGAATCCCCATTATTTGGATCTGCTGGTGGCAAGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTGAATGAGCAGATCAAGCGTTTTCTTCTGTTGCTTACTGTCCAAGATACAGCAATGGACATTCCAACTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAATATGCCACATGCACCCATAGTGTCCAATATGGTGCCATTCAGTGTTATGACACCACATTACCTGGAGGATATTAATTTTTCAATGGAGGAGCTGCATTCAAGCCAGCAAGAAGTTTCTATCATCTTTTACATGCAAAAGATATTTCCAGTGGAAAAACTTCCTGGAGCGCATGGTTATGAAAATTTGGATGGGTTGGAGAAGGACAAAAAGGAAGAGCTTAGAAATTGGGCTTCTTTCCGGGGCCAAACACTGAGCAGAACAGTTAGAGGAATGATGTACTATAGAGAAGCCTTAAAACTACAAGCATTTCTTGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATGCATTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTTGTTACTTGCCAATTATTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAAAAGATACCCAACTCTCCGTGTTGCTTATGTCGAGGAGAAAGAAGAGATAGTGGAGAACAAGCCTTGCAAAGTTTATTCTTCAGTATTGGTTAAAGCTATCCCCAAGTATGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTGCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAACCATGGAATAATCTTCACTCGTGGTGAAGCTCTCCAAACCATTGATATGAACCAAGACAGTTATTTGGAGGAAGCCTTCAAAATGAGAAATCTTCTGCAAGAATTTCTTCAGAACCGAGGACGGCGACCTCCTATACTGCTTGGTTTGAGGGAACACATATTTACAGGAAGTGTTTCTTCTCTTGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTACGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGATTAAGCAAAGCATCAAAAACAATTAACCTGAGCGAGGATGTTTTTGCTGGGTATAATTCTACCTTACGACGGGGATGGATCACGTATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAGTTTGAAGCTAAGGTTGCTAATGGTAACAGTGAACAAACTATAAGCCGTGACATATACCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTACGGACAGCTATACCTAGTTCTCAGTGGCTTGGAAAAAGCTCTCATAATTGAGGCTAGACTGCAGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGTTCCTCAATGCACTAAAAGATTTTGTCCTGATGCAATTGCAGCTGGCTTCTGTTTTTTTCACGTTCTCATTTGGAACAAAAATCCATTATTACGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACCGGGCGTAAGGTTGTGGTGTTTCATACTAGCTTCACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTTGTCAAAGGATTTGAGTTACTGCTCCTGTTGATTGTTTATAACTTGTTTAGACGGTCCTATGAGAGCGACATGGCGTATGTATTAGTCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTCGCTCCATTTCTTTTTAATCCGTCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGATTGGAATAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCACCAAGATAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGAGTTCTAGGTTGTTTGAAATACTCCTTTCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCAGGAATTTTCTGGTTTATTTGCTTTCATGGATAGTGATCCTCGTTGTTTTCTTGCTAGTAAAGGCAGTGAACCTCGGAAGGCAAAAGTTCAGTGCTAGATATCACCTTGTGTTTAGGCTATTCAAAGCAATCCTTTTCTTGGGTGTTCTTTCCATCATAATAACTCTTTCTCATGTGTGTCATCTATCTTGGATGGACTTGGTTGTGTGCTGCCTAGCATTTTTGCCAACAGGGTGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAATACTGGATTGTGGGAATTCACCAGAGTACTTGCTAAAACATATGACTACGGAATGGGGGTTGTTCTCTTCACACCTATTGCTGTCTTGGCATGGCTTCCGATTATATCAGCATTCCAGACTCGATTCCTGTTCAATGAGGCCTTCAATAGGCACTTGCAGATCCAACCAATTCTTCAGGGGAAGAAGAAGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1930

Amino Acids

223.59

Weight (kDa)

8.71

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 112 1.9e-07 Vta1 like
FKS1_dom1 PF14288 326 - 437 1.6e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 879 - 1072 8.3e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1079 - 1150 1.3e-18 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1166 - 1556 6.7e-145 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1566 - 1748 2.2e-39 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1199, 4968
AatII GACGTC 1 cut(s) 4510
AccB1I GGYRCC 1 cut(s) 3328
AccB7I CCANNNNNTGG 4 cut(s) 1142, 1594, 3119, 3950
AccI GTMKAC 2 cut(s) 2069, 5286
AccII CGCG 3 cut(s) 369, 469, 2032
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 5 cut(s) 223, 644, 880, 2394, 4498
AclWI GGATC 7 cut(s) 2814, 3130, 3748, 4382, 5138, 5344, 5749
AcoI YGGCCR 2 cut(s) 2633, 4819
AcyI GRCGYC 1 cut(s) 4507
AdeI CACNNNGTG 1 cut(s) 5422
AflIII ACRYGT 2 cut(s) 1750, 2739
AjnI CCWGG 4 cut(s) 534, 3357, 3457, 3934
AjuI GAANNNNNNNTTGG 4 cut(s) 1419, 1451, 5751, 5783
AleI CACNNNNGTG 1 cut(s) 2340
AloI GAACNNNNNNTCC 4 cut(s) 1842, 1874, 4664, 4696
Alw21I GWGCWC 1 cut(s) 3943
Alw26I GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3753, 4178
Alw44I GTGCAC 1 cut(s) 3939
AlwI GGATC 7 cut(s) 2814, 3130, 3748, 4382, 5138, 5344, 5749
AlwNI CAGNNNCTG 2 cut(s) 535, 3083
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 8 cut(s) 147, 531, 1310, 2633, 3538, 4819, 5579, 5733
ApaLI GTGCAC 1 cut(s) 3939
AseI ATTAAT 2 cut(s) 1571, 3369
Asp700I GAANNNNTTC 5 cut(s) 693, 3453, 3630, 4083, 4150
AspA2I CCTAGG 1 cut(s) 2015
AspLEI GCGC 3 cut(s) 469, 2032, 3466
AsuC2I CCSGG 2 cut(s) 3536, 4861
AsuII TTCGAA 2 cut(s) 266, 2350
AvaII GGWCC 6 cut(s) 256, 524, 1942, 3775, 4983, 5200
AvrII CCTAGG 1 cut(s) 2015
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 3943
BalI TGGCCA 1 cut(s) 2635
BanI GGYRCC 1 cut(s) 3328
BanII GRGCYC 2 cut(s) 27, 2248
BarI GAAGNNNNNNTAC 4 cut(s) 1810, 1842, 2747, 2779
BauI CACGAG 1 cut(s) 3996
BbsI GAAGAC 5 cut(s) 1278, 2595, 2725, 2864, 2964
Bbv12I GWGCWC 1 cut(s) 3943
BccI CCATC 8 cut(s) 35, 1921, 2970, 3479, 4365, 5211, 5474, 5500
BceAI ACGGC 4 cut(s) 2816, 4115, 4470, 4834
BciT130I CCWGG 4 cut(s) 536, 3359, 3459, 3936
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 3 cut(s) 94, 595, 3907
BcnI CCSGG 2 cut(s) 3536, 4861
BcoDI GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3753, 4178
BfmI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3577, 4667, 4903
BfuI GTATCC 1 cut(s) 563
BglII AGATCT 3 cut(s) 691, 2905, 4431
BlnI CCTAGG 1 cut(s) 2015
BlpI GCTNAGC 1 cut(s) 3681
BmcAI AGTACT 2 cut(s) 740, 5622
Bme1390I CCNGG 6 cut(s) 536, 3359, 3459, 3536, 3936, 4861
Bme18I GGWCC 6 cut(s) 256, 524, 1942, 3775, 4983, 5200
BmiI GGNNCC 8 cut(s) 201, 1944, 2247, 2528, 3330, 3539, 4727, 5582
BmrFI CCNGG 6 cut(s) 536, 3359, 3459, 3536, 3936, 4861
BmrI ACTGGG 2 cut(s) 1864, 4699
BmuI ACTGGG 2 cut(s) 1864, 4699
BoxI GACNNNNGTC 1 cut(s) 839
BpiI GAAGAC 5 cut(s) 1278, 2595, 2725, 2864, 2964
BpmI CTGGAG 3 cut(s) 3380, 3480, 3777
Bpu10I CCTNAGC 3 cut(s) 111, 4325, 4449
Bpu1102I GCTNAGC 1 cut(s) 3681
Bpu14I TTCGAA 2 cut(s) 266, 2350
BpuEI CTTGAG 1 cut(s) 2897
BpuMI CCSGG 2 cut(s) 3536, 4861
BsaAI YACGTR 1 cut(s) 4380
BsaBI GATNNNNATC 2 cut(s) 99, 5348
BsaHI GRCGYC 1 cut(s) 4507
BsaI GGTCTC 3 cut(s) 1975, 3753, 4178
BsaWI WCCGGW 2 cut(s) 159, 939
BsaXI ACNNNNNCTCC 2 cut(s) 1333, 1363
Bse118I RCCGGY 1 cut(s) 521
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 1695, 1779, 3234
Bse8I GATNNNNATC 2 cut(s) 99, 5348
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 4 cut(s) 536, 3359, 3459, 3936
BseJI GATNNNNATC 2 cut(s) 99, 5348
BseMI GCAATG 3 cut(s) 1695, 1779, 3234
BseRI GAGGAG 5 cut(s) 212, 1436, 2383, 3398, 3842
BseSI GKGCMC 1 cut(s) 3943
BseX3I CGGCCG 1 cut(s) 4819
BseYI CCCAGC 1 cut(s) 4343
Bsh1236I CGCG 3 cut(s) 369, 469, 2032
Bsh1285I CGRYCG 1 cut(s) 4822
BshFI GGCC 8 cut(s) 149, 533, 1312, 2635, 3540, 4821, 5581, 5735
BshNI GGYRCC 1 cut(s) 3328
BsiEI CGRYCG 1 cut(s) 4822
BsiHKAI GWGCWC 1 cut(s) 3943
BsiSI CCGG 6 cut(s) 160, 522, 940, 2619, 3535, 4860
BslFI GGGAC 4 cut(s) 721, 1243, 1928, 5105
BsmAI GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3753, 4178
BsmBI CGTCTC 1 cut(s) 3253
BsmFI GGGAC 4 cut(s) 721, 1243, 1928, 5105
BsmI GAATGC 4 cut(s) 1378, 2396, 3391, 5705
BsnI GGCC 8 cut(s) 149, 533, 1312, 2635, 3540, 4821, 5581, 5735
Bso31I GGTCTC 3 cut(s) 1975, 3753, 4178
Bsp119I TTCGAA 2 cut(s) 266, 2350
Bsp1286I GDGCHC 3 cut(s) 27, 2248, 3943
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3681
Bsp19I CCATGG 2 cut(s) 78, 3979
BspACI CCGC 5 cut(s) 223, 644, 880, 2394, 4498
BspANI GGCC 8 cut(s) 149, 533, 1312, 2635, 3540, 4821, 5581, 5735
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 3 cut(s) 369, 469, 2032
BspHI TCATGA 3 cut(s) 1641, 2160, 4384
BspLI GGNNCC 8 cut(s) 201, 1944, 2247, 2528, 3330, 3539, 4727, 5582
BspMAI CTGCAG 3 cut(s) 309, 865, 4671
BspPI GGATC 7 cut(s) 2814, 3130, 3748, 4382, 5138, 5344, 5749
BspQI GCTCTTC 1 cut(s) 3504
BspT104I TTCGAA 2 cut(s) 266, 2350
BspT107I GGYRCC 1 cut(s) 3328
BspTNI GGTCTC 3 cut(s) 1975, 3753, 4178
BsrDI GCAATG 3 cut(s) 1695, 1779, 3234
BsrFI RCCGGY 1 cut(s) 521
BssAI RCCGGY 1 cut(s) 521
BssNAI GTATAC 1 cut(s) 2070
BssNI GRCGYC 1 cut(s) 4507
BssSI CACGAG 1 cut(s) 3996
BssT1I CCWWGG 6 cut(s) 78, 2015, 2338, 3979, 4501, 4683
Bst1107I GTATAC 1 cut(s) 2070
Bst2BI CACGAG 1 cut(s) 3996
Bst2UI CCWGG 4 cut(s) 536, 3359, 3459, 3936
Bst6I CTCTTC 4 cut(s) 1882, 3504, 3831, 5666
BstACI GRCGYC 1 cut(s) 4507
BstAPI GCANNNNNTGC 2 cut(s) 1388, 3677
BstBAI YACGTR 1 cut(s) 4380
BstBI TTCGAA 2 cut(s) 266, 2350
BstC8I GCNNGC 5 cut(s) 1137, 1585, 2051, 3401, 4770
BstDSI CCRYGG 2 cut(s) 78, 3979
BstEII GGTNACC 1 cut(s) 942
BstENI CCTNNNNNAGG 3 cut(s) 2425, 4328, 5740
BstFNI CGCG 3 cut(s) 369, 469, 2032
BstHHI GCGC 3 cut(s) 469, 2032, 3466
BstMAI GTCTC 7 cut(s) 847, 1052, 1711, 1975, 3253, 3753, 4178
BstMCI CGRYCG 1 cut(s) 4822
BstNI CCWGG 4 cut(s) 536, 3359, 3459, 3936
BstNSI RCATGY 8 cut(s) 1093, 1587, 1754, 2743, 3308, 3430, 3770, 4396
BstPAI GACNNNNGTC 1 cut(s) 839
BstPI GGTNACC 1 cut(s) 942
BstSCI CCNGG 6 cut(s) 534, 3357, 3457, 3534, 3934, 4859
BstSFI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3577, 4667, 4903
BstSLI GKGCMC 1 cut(s) 3943
BstUI CGCG 3 cut(s) 369, 469, 2032
BstV2I GAAGAC 5 cut(s) 1278, 2595, 2725, 2864, 2964
BstX2I RGATCY 6 cut(s) 691, 2806, 2905, 3122, 4431, 5754
BstXI CCANNNNNNTGG 4 cut(s) 1474, 3737, 3980, 5547
BstYI RGATCY 6 cut(s) 691, 2806, 2905, 3122, 4431, 5754
BstZ17I GTATAC 1 cut(s) 2070
BstZI CGGCCG 1 cut(s) 4819
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 8 cut(s) 149, 533, 1312, 2635, 3540, 4821, 5581, 5735
BtgI CCRYGG 2 cut(s) 78, 3979
BtsI GCAGTG 4 cut(s) 91, 870, 1386, 5385
Cac8I GCNNGC 5 cut(s) 1137, 1585, 2051, 3401, 4770
CaiI CAGNNNCTG 2 cut(s) 535, 3083
CciI TCATGA 3 cut(s) 1641, 2160, 4384
CfoI GCGC 3 cut(s) 469, 2032, 3466
Cfr10I RCCGGY 1 cut(s) 521
CseI GACGC 3 cut(s) 387, 1307, 3270
CspCI CAANNNNNGTGG 4 cut(s) 1593, 1628, 2406, 2441
DraI TTTAAA 1 cut(s) 892
DraIII CACNNNGTG 1 cut(s) 5422
EaeI YGGCCR 2 cut(s) 2633, 4819
EagI CGGCCG 1 cut(s) 4819
Eam1104I CTCTTC 4 cut(s) 1882, 3504, 3831, 5666
EarI CTCTTC 4 cut(s) 1882, 3504, 3831, 5666
EclXI CGGCCG 1 cut(s) 4819
Eco130I CCWWGG 6 cut(s) 78, 2015, 2338, 3979, 4501, 4683
Eco147I AGGCCT 1 cut(s) 5735
Eco24I GRGCYC 2 cut(s) 27, 2248
Eco31I GGTCTC 3 cut(s) 1975, 3753, 4178
Eco32I GATATC 3 cut(s) 1639, 4578, 5414
Eco47I GGWCC 6 cut(s) 256, 524, 1942, 3775, 4983, 5200
Eco52I CGGCCG 1 cut(s) 4819
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 942
EcoNI CCTNNNNNAGG 3 cut(s) 2425, 4328, 5740
EcoO109I RGGNCCY 3 cut(s) 532, 3775, 5580
EcoO65I GGTNACC 1 cut(s) 942
EcoRI GAATTC 2 cut(s) 3139, 5609
EcoRII CCWGG 4 cut(s) 534, 3357, 3457, 3934
EcoRV GATATC 3 cut(s) 1639, 4578, 5414
EcoT14I CCWWGG 6 cut(s) 78, 2015, 2338, 3979, 4501, 4683
EcoT22I ATGCAT 2 cut(s) 3673, 3694
EcoT38I GRGCYC 2 cut(s) 27, 2248
ErhI CCWWGG 6 cut(s) 78, 2015, 2338, 3979, 4501, 4683
Esp3I CGTCTC 1 cut(s) 3253
FaqI GGGAC 4 cut(s) 721, 1243, 1928, 5105
FauNDI CATATG 5 cut(s) 1068, 1075, 1141, 3715, 5635
FbaI TGATCA 3 cut(s) 94, 595, 3907
FblI GTMKAC 2 cut(s) 2069, 5286
FriOI GRGCYC 2 cut(s) 27, 2248
GlaI GCGC 3 cut(s) 468, 2031, 3465
GsaI CCCAGC 1 cut(s) 4347
GsuI CTGGAG 3 cut(s) 3380, 3480, 3777
HaeIII GGCC 8 cut(s) 149, 533, 1312, 2635, 3540, 4821, 5581, 5735
HapII CCGG 6 cut(s) 160, 522, 940, 2619, 3535, 4860
HgaI GACGC 3 cut(s) 387, 1307, 3270
HhaI GCGC 3 cut(s) 469, 2032, 3466
Hin1I GRCGYC 1 cut(s) 4507
Hin6I GCGC 3 cut(s) 467, 2030, 3464
HinP1I GCGC 3 cut(s) 467, 2030, 3464
HincII GTYRAC 1 cut(s) 1095
HindII GTYRAC 1 cut(s) 1095
HindIII AAGCTT 2 cut(s) 1525, 3929
HpaI GTTAAC 1 cut(s) 1095
HpaII CCGG 6 cut(s) 160, 522, 940, 2619, 3535, 4860
Hpy99I CGWCG 2 cut(s) 264, 4368
HpyCH4IV ACGT 4 cut(s) 1501, 4379, 4507, 4787
HpySE526I ACGT 4 cut(s) 1501, 4379, 4507, 4787
Hsp92I GRCGYC 1 cut(s) 4507
HspAI GCGC 3 cut(s) 467, 2030, 3464
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 3 cut(s) 94, 595, 3907
KspAI GTTAAC 1 cut(s) 1095
LguI GCTCTTC 1 cut(s) 3504
MaeII ACGT 4 cut(s) 1501, 4379, 4507, 4787
MaeIII GTNAC 9 cut(s) 443, 942, 1441, 3721, 4195, 4463, 4487, 4944, 5014
MfeI CAATTG 1 cut(s) 4760
MflI RGATCY 6 cut(s) 691, 2806, 2905, 3122, 4431, 5754
MhlI GDGCHC 3 cut(s) 27, 2248, 3943
MlsI TGGCCA 1 cut(s) 2635
MluNI TGGCCA 1 cut(s) 2635
MlyI GAGTC 4 cut(s) 1166, 1340, 2325, 5707
MmeI TCCRAC 4 cut(s) 1376, 3263, 3471, 5782
Mox20I TGGCCA 1 cut(s) 2635
Mph1103I ATGCAT 2 cut(s) 3673, 3694
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 5 cut(s) 693, 3453, 3630, 4083, 4150
MscI TGGCCA 1 cut(s) 2635
MslI CAYNNNNRTG 5 cut(s) 2340, 3471, 4022, 4257, 4839
Msp20I TGGCCA 1 cut(s) 2635
MspA1I CMGCKG 2 cut(s) 1235, 4768
MspI CCGG 6 cut(s) 160, 522, 940, 2619, 3535, 4860
MspR9I CCNGG 6 cut(s) 536, 3359, 3459, 3536, 3936, 4861
MunI CAATTG 1 cut(s) 4760
Mva1269I GAATGC 4 cut(s) 1378, 2396, 3391, 5705
MvaI CCWGG 4 cut(s) 536, 3359, 3459, 3936
MvnI CGCG 3 cut(s) 369, 469, 2032
NciI CCSGG 2 cut(s) 3536, 4861
NcoI CCATGG 2 cut(s) 78, 3979
NdeI CATATG 5 cut(s) 1068, 1075, 1141, 3715, 5635
NlaIV GGNNCC 8 cut(s) 201, 1944, 2247, 2528, 3330, 3539, 4727, 5582
NmeAIII GCCGAG 1 cut(s) 1237
NmuCI GTSAC 4 cut(s) 443, 4195, 4487, 5014
NsiI ATGCAT 2 cut(s) 3673, 3694
NspI RCATGY 8 cut(s) 1093, 1587, 1754, 2743, 3308, 3430, 3770, 4396
NspV TTCGAA 2 cut(s) 266, 2350
OliI CACNNNNGTG 1 cut(s) 2340
PaeI GCATGC 1 cut(s) 1587
PagI TCATGA 3 cut(s) 1641, 2160, 4384
PceI AGGCCT 1 cut(s) 5735
PciI ACATGT 2 cut(s) 1750, 2739
PciSI GCTCTTC 1 cut(s) 3504
PcsI WCGNNNNNNNCGW 1 cut(s) 667
PctI GAATGC 4 cut(s) 1378, 2396, 3391, 5705
PdmI GAANNNNTTC 5 cut(s) 693, 3453, 3630, 4083, 4150
PflMI CCANNNNNTGG 4 cut(s) 1142, 1594, 3119, 3950
PfoI TCCNGGA 1 cut(s) 3457
PleI GAGTC 4 cut(s) 1165, 1340, 2324, 5707
PpsI GAGTC 4 cut(s) 1165, 1340, 2324, 5707
Ppu21I YACGTR 1 cut(s) 4380
PpuMI RGGWCCY 1 cut(s) 3775
PscI ACATGT 2 cut(s) 1750, 2739
PshAI GACNNNNGTC 1 cut(s) 839
PshBI ATTAAT 2 cut(s) 1571, 3369
PsiI TTATAA 2 cut(s) 1199, 4968
Psp5II RGGWCCY 1 cut(s) 3775
Psp6I CCWGG 4 cut(s) 534, 3357, 3457, 3934
PspEI GGTNACC 1 cut(s) 942
PspFI CCCAGC 1 cut(s) 4343
PspGI CCWGG 4 cut(s) 534, 3357, 3457, 3934
PspN4I GGNNCC 8 cut(s) 201, 1944, 2247, 2528, 3330, 3539, 4727, 5582
PspPPI RGGWCCY 1 cut(s) 3775
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 3 cut(s) 309, 865, 4671
PstNI CAGNNNCTG 2 cut(s) 535, 3083
PsuI RGATCY 6 cut(s) 691, 2806, 2905, 3122, 4431, 5754
PvuII CAGCTG 2 cut(s) 1235, 4768
RseI CAYNNNNRTG 5 cut(s) 2340, 3471, 4022, 4257, 4839
SapI GCTCTTC 1 cut(s) 3504
ScaI AGTACT 2 cut(s) 740, 5622
SchI GAGTC 4 cut(s) 1166, 1340, 2325, 5707
ScrFI CCNGG 6 cut(s) 536, 3359, 3459, 3536, 3936, 4861
SduI GDGCHC 3 cut(s) 27, 2248, 3943
SfcI CTRYAG 7 cut(s) 305, 861, 1395, 1785, 3577, 4667, 4903
SfuI TTCGAA 2 cut(s) 266, 2350
SinI GGWCC 6 cut(s) 256, 524, 1942, 3775, 4983, 5200
SmiMI CAYNNNNRTG 5 cut(s) 2340, 3471, 4022, 4257, 4839
SmlI CTYRAG 1 cut(s) 2876
SmoI CTYRAG 1 cut(s) 2876
SphI GCATGC 1 cut(s) 1587
SseBI AGGCCT 1 cut(s) 5735
SsiI CCGC 5 cut(s) 223, 644, 880, 2394, 4498
SspI AATATT 3 cut(s) 583, 808, 2404
StuI AGGCCT 1 cut(s) 5735
StyD4I CCNGG 6 cut(s) 534, 3357, 3457, 3534, 3934, 4859
StyI CCWWGG 6 cut(s) 78, 2015, 2338, 3979, 4501, 4683
TaiI ACGT 4 cut(s) 1504, 4382, 4510, 4790
TatI WGTACW 9 cut(s) 402, 738, 2089, 2262, 2736, 2742, 2778, 3573, 5620
TauI GCSGC 2 cut(s) 225, 647
TseFI GTSAC 4 cut(s) 443, 4195, 4487, 5014
Tsp45I GTSAC 4 cut(s) 443, 4195, 4487, 5014
TspGWI ACGGA 8 cut(s) 361, 832, 1448, 1863, 3800, 4623, 5064, 5658
Van91I CCANNNNNTGG 4 cut(s) 1142, 1594, 3119, 3950
VneI GTGCAC 1 cut(s) 3939
VpaK11BI GGWCC 6 cut(s) 256, 524, 1942, 3775, 4983, 5200
VspI ATTAAT 2 cut(s) 1571, 3369
XagI CCTNNNNNAGG 3 cut(s) 2425, 4328, 5740
XbaI TCTAGA 2 cut(s) 1321, 1814
XceI RCATGY 8 cut(s) 1093, 1587, 1754, 2743, 3308, 3430, 3770, 4396
XcmI CCANNNNNNNNNTGG 2 cut(s) 2184, 2515
XmaJI CCTAGG 1 cut(s) 2015
XmiI GTMKAC 2 cut(s) 2069, 5286
XmnI GAANNNNTTC 5 cut(s) 693, 3453, 3630, 4083, 4150
ZraI GACGTC 1 cut(s) 4508
ZrmI AGTACT 2 cut(s) 740, 5622
Zsp2I ATGCAT 2 cut(s) 3673, 3694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.