RchiOBHm_Chr5g0028271

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016003
Physical Location & Seq
Forward (+)
22108394 .. 22124882
16489 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30772

Sequence Viewer

Length: 5841 bp
ATGTCTGAGATTGTGGTTGCAGAGCCCATCTATGATGATCGAGCAGAGGAAAAAAGACCCATTACCAGGTATCTCACCATGGGCAGTGGGAGTGATCATAATCATCCTCCTGAGCCATTTGAGAGCGAGAGATTGCCTGCCACTTTGGCCTCAAAAATCCGGAGCTTTCTTCGGGTTGCAAACTTGATCGAAAGCGAGGAGCCTCGGATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCGTTCGAATGGGAGGGGTGTTCGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACAATTAGGAAACGGAAGGAAAAGAGTGATCTACGCGAGTTAAGACGCGTTTATCATTCTTACAAGGAGTACATCATCAAACATGAAGGAGCATTTAGCATAGAAAATAGAGAGAAGTTGATAAATGCGCGTATAATTGGTTCTGTATTGTTTGAAGTCTTAAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTGGCTAATAGAGGTGGTATCCAGACAAAACCTTATGATATATATAATATTCTACCACTTGATCAAGGAGGTATTCAGCAACCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAATACGGTATATTCGTGGCATACCTTCCAATGAAGATCTTCAGAAACATGGGACTTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTCTACTCCTTGCTAATATTCACAAACGGAAAACTCATAAACAGACATCTGTCTCAAAATTAGGAGATGCTGCAGTGGACGAGTTAATGCGGAGAATTTTTAAAAATTATACAAACTGGTGCAAGTTTTTGGGAAGGAAAAGCAACATCCGGTTACCTTATGTGAAACAAGAAGCCCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGCGAGGCTGCAAACTTGCGATTCATGCCAGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAGGTCATGCCAGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTTGTTGCCCCCATATATGGGGTTATAAGAGAGGAAGCCGAGAAAAGCAAGTGTGGGACAGCTGACCATTCCACATGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTTCTGATTGTTTTGAAATCGGTTGGCCCATGCGTCTAGATCACGATTTCTTCTTTACAGACTCTCCAACAAAATCCAATGCTAAGAATGCTTCAGCATCCACTGCTCCTGTAGAAGAAAGAAGGAAGGAAGATGAGGAGGAAGATGAAGTTGGGGTTAGTAAAGAAGAAGTCCGTGAACCCAAATGGTTGGGCAAAACAAATTTCGTAGAAGTACGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCACTTCAGGCATTAATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAGTTGTTTGACAAAGTTATATTTGAGGATATCATGAGCATCTTCATTACATCTGCATTTCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACACTGGACTTCTCTGCAAAAGTAAAACATGTTATGAAGCTCGGTGTTGCAATGATATGGACTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATCCCACAACATACGGAAGCTGGGTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATTTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTATTCGCAAATACATTGAGGTCTCAAATTACCAAATATGCACCATTCTGTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGATTGCAAGAAAGCCAGCTTTCAGTGTTGAAGTATACATTGTTTTGGGTACTTGTACTATTGAGCAAATTTTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCCAATGTATGATTGGCATGAGCTTTTTCCGAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGAACTTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCTGCATTCAATATTTCTCTTATTCCACCCTCATTGAGGAATGACCAAAAGAGAAGAAAAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAGACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTCAGGACTTTAGGATGATTAAAGTTCCAGATCTTTTGGCTAAATGTATCGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCGTGGCAAAGTTGTGAAAGTTCTCCAAGATATATTTGAGCTTGTAATCAATGATATGATGACGACTGGCTTCAGGATATTAGAGTTGCTGGATTCATCCCAACAGACTGAAACAGATGCTGCTTATTTTTCTGGAAGCATTGAATCCCCATTATTTGGATCTGCTGGTGGCAAGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTGAATGAGCAGATCAAGCGTTTTCTTCTGTTGCTTACTGTCCAAGATACAGCAATGGACATTCCAACTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAATATGCCACATGCACCCACAGTGTCCAATATGGTGCCATTCAGTGTTATGACACCACATTACCTGGAGGATATTAATTTTTCAATGGAGGAGCTGCATTCAAGCCAGCAAGAAGTTTCTATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCCTGGAGCGCATGGGTTATGAAAATTTGGATGGGTTGGAGAAGGACAAAAAGGAAGAGCTTAGAAATTGGGCTTCTTTCCGGGGCCAAACACTGAGCAGAACAGTTAGAGGAATGATGTACTATAGAGAAGCCTTAAAACTGCAAGCATTTCTTGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATGCATTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACTTATGTTGTTACCTGCCAATTATTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAAAAGATACCCAACTCTCCGTGTTGCTTATGTTGAGGAGAAAGAAGAGATAGTGGAGAACAAGCCTTGCAAAGTTTATTCTTCAGTATTGGTTAAAGCTATCCCCAAGTATGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTGCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAACCATGGAATAATCTTCACTCGTGGTGAAGCTCTCCAAACCATTGATATGAACCAAGACAGTTATTTGGAGGAAGCCTTCAAAATGAGAAATCTTCTGCAAGAATTTCTTCAGAACCGAGGACGGCGACCTCCTATACTGCTTGGTTTGAGGGAACACATATTTACAGGAAGTGTTTCTTCTCTTGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTACGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGATTAAGCAAAGCATCAAAAACAATTAACCTGAGCGAGGATGTTTTTGCTGGGTATAATTCTACCTTACGACGGGGATGGATCACGTATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAGTTTGAAGCTAAGGTTGCTAATGGTAACAGTGAACAAACTTTAAGCCGTGACATATACCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTACGGACAGCTATACCTAGTTCTCAGTGGCTTGGAAAAAGCTCTCATAATTGAGGCTAGACTGCAGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGCTATTAACAGGAATGCCAATGGTGATGGAAATTGGACTTGAGAAAGGGTTCCTCAATGCACTAAAAGATTTTGTCCTGATGCAATTGCAGCTGGCTTCTGTTTTTTTCACGTTCTCATTTGGAACAAAAATCCATTATTACGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTTGTTGTGTTTCATACTAGCTTCACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTCGTCAAAGGATTTGAGTTACTGCTCCTGTTGATTGTTTATAACTTGTTTAGACGGTCCTATGAGAGCGACATGGCGTATGTATTAGTCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTCGCTCCATTTCTTTTTAATCCGTCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGATTGGAATAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCACCAAGATAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGAGTTCTAGGTTGTTTGAAATACTCCTTTCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCAGGAATTTTCTGGTTTATTTGCTTTCATGGATAGTGATCCTCGTTGTTTTCTTGTTAGTAAAGGCAGTGAACCTCGGAAGGCAAAAGTTCAGTGCTAGATATCACCTTGTGTTTAGGCTATTCAAAGCAACCCTTTTCTTGGGTGTTCTTTCCATCATAATAACTCTTTCTCATGTGTGTCATCTATCTTGGATGGACTTGGTTGTGTGCTGCCTAGCATTTTTGCCAACAGGGTGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAATACTGGATTGTGGGAATTCACCAGAGTACTTGCTAAAACATATGACTACGGAATGGGGGTTGTTCTCTTTACACCTATTGCTGTCTTGGCATGGCTTCCGATTATATCAGCATTCCAGACTCGATTCCTGTTTAATGAGGCCTTCAATAGGCACTTGCAGATCCAACCAATTCTTCAGGGGAAGAAGAAGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1946

Amino Acids

225.49

Weight (kDa)

8.6

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 112 2.2e-07 Vta1 like
FKS1_dom1 PF14288 324 - 435 1.6e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 877 - 1070 3.1e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1077 - 1162 4.8e-25 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1167 - 1767 7.8e-195 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1193, 5016
AatII GACGTC 1 cut(s) 4510
Acc36I ACCTGC 1 cut(s) 3734
AccB1I GGYRCC 1 cut(s) 3322
AccB7I CCANNNNNTGG 4 cut(s) 1136, 1588, 3113, 3950
AccI GTMKAC 2 cut(s) 2063, 5334
AccII CGCG 4 cut(s) 369, 381, 463, 2026
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 4 cut(s) 223, 638, 874, 4498
AclWI GGATC 7 cut(s) 2808, 3124, 3748, 4382, 5186, 5392, 5797
AcoI YGGCCR 2 cut(s) 2627, 4867
AcyI GRCGYC 1 cut(s) 4507
AdeI CACNNNGTG 1 cut(s) 5470
AflIII ACRYGT 3 cut(s) 379, 1744, 2733
AjnI CCWGG 5 cut(s) 65, 528, 3351, 3456, 3934
AjuI GAANNNNNNNTTGG 4 cut(s) 1413, 1445, 5799, 5831
AleI CACNNNNGTG 1 cut(s) 2334
AloI GAACNNNNNNTCC 4 cut(s) 1836, 1868, 4664, 4696
Alw21I GWGCWC 1 cut(s) 3943
Alw26I GTCTC 7 cut(s) 841, 1046, 1705, 1969, 3247, 3753, 4178
Alw44I GTGCAC 1 cut(s) 3939
AlwI GGATC 7 cut(s) 2808, 3124, 3748, 4382, 5186, 5392, 5797
AlwNI CAGNNNCTG 2 cut(s) 529, 3077
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 8 cut(s) 147, 525, 1304, 2627, 3538, 4867, 5627, 5781
ApaLI GTGCAC 1 cut(s) 3939
AseI ATTAAT 2 cut(s) 1565, 3363
Asp700I GAANNNNTTC 6 cut(s) 687, 3452, 3630, 4083, 4150, 4772
AspA2I CCTAGG 1 cut(s) 2009
AspLEI GCGC 3 cut(s) 463, 2026, 3465
AsuC2I CCSGG 1 cut(s) 3536
AsuII TTCGAA 2 cut(s) 266, 2344
AvaII GGWCC 6 cut(s) 256, 518, 1936, 3775, 5031, 5248
AvrII CCTAGG 1 cut(s) 2009
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 3943
BalI TGGCCA 1 cut(s) 2629
BanI GGYRCC 1 cut(s) 3322
BanII GRGCYC 2 cut(s) 27, 2242
BarI GAAGNNNNNNTAC 4 cut(s) 1804, 1836, 2741, 2773
BauI CACGAG 1 cut(s) 3996
BbsI GAAGAC 5 cut(s) 1272, 2589, 2719, 2858, 2958
Bbv12I GWGCWC 1 cut(s) 3943
BccI CCATC 9 cut(s) 35, 1915, 2964, 3479, 4365, 4744, 5259, 5522, 5548
BceAI ACGGC 3 cut(s) 4115, 4470, 4882
BciT130I CCWGG 5 cut(s) 67, 530, 3353, 3458, 3936
BciVI GTATCC 1 cut(s) 557
BclI TGATCA 3 cut(s) 94, 589, 3907
BcnI CCSGG 1 cut(s) 3536
BcoDI GTCTC 7 cut(s) 841, 1046, 1705, 1969, 3247, 3753, 4178
BfmI CTRYAG 7 cut(s) 305, 855, 1389, 1779, 3577, 4667, 4951
BfuAI ACCTGC 1 cut(s) 3734
BfuI GTATCC 1 cut(s) 557
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 3 cut(s) 685, 2899, 4431
BlnI CCTAGG 1 cut(s) 2009
BlpI GCTNAGC 1 cut(s) 3681
BmcAI AGTACT 2 cut(s) 734, 5670
Bme1390I CCNGG 6 cut(s) 67, 530, 3353, 3458, 3536, 3936
Bme18I GGWCC 6 cut(s) 256, 518, 1936, 3775, 5031, 5248
BmiI GGNNCC 8 cut(s) 201, 1938, 2241, 2522, 3324, 3539, 4775, 5630
BmrFI CCNGG 6 cut(s) 67, 530, 3353, 3458, 3536, 3936
BmrI ACTGGG 2 cut(s) 4699, 4917
BmuI ACTGGG 2 cut(s) 4699, 4917
BoxI GACNNNNGTC 1 cut(s) 833
BpiI GAAGAC 5 cut(s) 1272, 2589, 2719, 2858, 2958
BpmI CTGGAG 3 cut(s) 3374, 3479, 3777
Bpu10I CCTNAGC 3 cut(s) 111, 4325, 4449
Bpu1102I GCTNAGC 1 cut(s) 3681
Bpu14I TTCGAA 2 cut(s) 266, 2344
BpuEI CTTGAG 1 cut(s) 4784
BpuMI CCSGG 1 cut(s) 3536
BsaAI YACGTR 1 cut(s) 4380
BsaBI GATNNNNATC 2 cut(s) 99, 5396
BsaHI GRCGYC 1 cut(s) 4507
BsaI GGTCTC 3 cut(s) 1969, 3753, 4178
BsaWI WCCGGW 2 cut(s) 159, 933
BsaXI ACNNNNNCTCC 2 cut(s) 1327, 1357
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 9 cut(s) 905, 1096, 1725, 2630, 3028, 4705, 4912, 5162, 5650
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 3 cut(s) 1689, 1773, 3228
Bse8I GATNNNNATC 2 cut(s) 99, 5396
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 5 cut(s) 67, 530, 3353, 3458, 3936
BseJI GATNNNNATC 2 cut(s) 99, 5396
BseMI GCAATG 3 cut(s) 1689, 1773, 3228
BseNI ACTGG 9 cut(s) 905, 1096, 1725, 2630, 3028, 4705, 4912, 5162, 5650
BseRI GAGGAG 5 cut(s) 212, 1430, 2377, 3392, 3842
BseSI GKGCMC 1 cut(s) 3943
BseX3I CGGCCG 1 cut(s) 4867
BseYI CCCAGC 2 cut(s) 1848, 4343
Bsh1236I CGCG 4 cut(s) 369, 381, 463, 2026
Bsh1285I CGRYCG 1 cut(s) 4870
BshFI GGCC 8 cut(s) 149, 527, 1306, 2629, 3540, 4869, 5629, 5783
BshNI GGYRCC 1 cut(s) 3322
BsiEI CGRYCG 1 cut(s) 4870
BsiHKAI GWGCWC 1 cut(s) 3943
BsiSI CCGG 5 cut(s) 160, 516, 934, 2613, 3535
BslFI GGGAC 4 cut(s) 715, 1237, 1922, 5153
BsmAI GTCTC 7 cut(s) 841, 1046, 1705, 1969, 3247, 3753, 4178
BsmBI CGTCTC 1 cut(s) 3247
BsmFI GGGAC 4 cut(s) 715, 1237, 1922, 5153
BsmI GAATGC 5 cut(s) 1372, 2390, 3385, 4743, 5753
BsnI GGCC 8 cut(s) 149, 527, 1306, 2629, 3540, 4869, 5629, 5783
Bso31I GGTCTC 3 cut(s) 1969, 3753, 4178
Bsp119I TTCGAA 2 cut(s) 266, 2344
Bsp1286I GDGCHC 3 cut(s) 27, 2242, 3943
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3681
Bsp19I CCATGG 2 cut(s) 78, 3979
BspACI CCGC 4 cut(s) 223, 638, 874, 4498
BspANI GGCC 8 cut(s) 149, 527, 1306, 2629, 3540, 4869, 5629, 5783
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 4 cut(s) 369, 381, 463, 2026
BspHI TCATGA 3 cut(s) 1635, 2154, 4384
BspLI GGNNCC 8 cut(s) 201, 1938, 2241, 2522, 3324, 3539, 4775, 5630
BspMAI CTGCAG 3 cut(s) 309, 859, 4671
BspMI ACCTGC 1 cut(s) 3734
BspPI GGATC 7 cut(s) 2808, 3124, 3748, 4382, 5186, 5392, 5797
BspQI GCTCTTC 1 cut(s) 3504
BspT104I TTCGAA 2 cut(s) 266, 2344
BspT107I GGYRCC 1 cut(s) 3322
BspTNI GGTCTC 3 cut(s) 1969, 3753, 4178
BsrDI GCAATG 3 cut(s) 1689, 1773, 3228
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 9 cut(s) 905, 1096, 1725, 2630, 3028, 4705, 4912, 5162, 5650
BssAI RCCGGY 1 cut(s) 515
BssNAI GTATAC 1 cut(s) 2064
BssNI GRCGYC 1 cut(s) 4507
BssSI CACGAG 1 cut(s) 3996
BssT1I CCWWGG 6 cut(s) 78, 2009, 2332, 3979, 4501, 4683
Bst1107I GTATAC 1 cut(s) 2064
Bst2BI CACGAG 1 cut(s) 3996
Bst2UI CCWGG 5 cut(s) 67, 530, 3353, 3458, 3936
Bst6I CTCTTC 3 cut(s) 1876, 3504, 3831
BstACI GRCGYC 1 cut(s) 4507
BstAPI GCANNNNNTGC 2 cut(s) 1382, 3677
BstBAI YACGTR 1 cut(s) 4380
BstBI TTCGAA 2 cut(s) 266, 2344
BstC8I GCNNGC 7 cut(s) 138, 1131, 1579, 2045, 3395, 3600, 4818
BstDSI CCRYGG 2 cut(s) 78, 3979
BstEII GGTNACC 1 cut(s) 936
BstENI CCTNNNNNAGG 3 cut(s) 2419, 4328, 5788
BstFNI CGCG 4 cut(s) 369, 381, 463, 2026
BstHHI GCGC 3 cut(s) 463, 2026, 3465
BstMAI GTCTC 7 cut(s) 841, 1046, 1705, 1969, 3247, 3753, 4178
BstMCI CGRYCG 1 cut(s) 4870
BstNI CCWGG 5 cut(s) 67, 530, 3353, 3458, 3936
BstNSI RCATGY 8 cut(s) 1087, 1581, 1748, 2737, 3302, 3424, 3770, 4396
BstPAI GACNNNNGTC 1 cut(s) 833
BstPI GGTNACC 1 cut(s) 936
BstSCI CCNGG 6 cut(s) 65, 528, 3351, 3456, 3534, 3934
BstSFI CTRYAG 7 cut(s) 305, 855, 1389, 1779, 3577, 4667, 4951
BstSLI GKGCMC 1 cut(s) 3943
BstUI CGCG 4 cut(s) 369, 381, 463, 2026
BstV2I GAAGAC 5 cut(s) 1272, 2589, 2719, 2858, 2958
BstX2I RGATCY 6 cut(s) 685, 2800, 2899, 3116, 4431, 5802
BstXI CCANNNNNNTGG 5 cut(s) 1468, 3444, 3737, 3980, 5595
BstYI RGATCY 6 cut(s) 685, 2800, 2899, 3116, 4431, 5802
BstZ17I GTATAC 1 cut(s) 2064
BstZI CGGCCG 1 cut(s) 4867
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 8 cut(s) 149, 527, 1306, 2629, 3540, 4869, 5629, 5783
BtgI CCRYGG 2 cut(s) 78, 3979
BtsI GCAGTG 4 cut(s) 91, 864, 1380, 5433
BveI ACCTGC 1 cut(s) 3734
Cac8I GCNNGC 7 cut(s) 138, 1131, 1579, 2045, 3395, 3600, 4818
CaiI CAGNNNCTG 2 cut(s) 529, 3077
CciI TCATGA 3 cut(s) 1635, 2154, 4384
CfoI GCGC 3 cut(s) 463, 2026, 3465
Cfr10I RCCGGY 1 cut(s) 515
CseI GACGC 3 cut(s) 387, 1301, 3264
CsiI ACCWGGT 1 cut(s) 65
CspCI CAANNNNNGTGG 4 cut(s) 1587, 1622, 2400, 2435
DraI TTTAAA 1 cut(s) 886
DraIII CACNNNGTG 1 cut(s) 5470
EaeI YGGCCR 2 cut(s) 2627, 4867
EagI CGGCCG 1 cut(s) 4867
Eam1104I CTCTTC 3 cut(s) 1876, 3504, 3831
EarI CTCTTC 3 cut(s) 1876, 3504, 3831
EclXI CGGCCG 1 cut(s) 4867
Eco130I CCWWGG 6 cut(s) 78, 2009, 2332, 3979, 4501, 4683
Eco147I AGGCCT 1 cut(s) 5783
Eco24I GRGCYC 2 cut(s) 27, 2242
Eco31I GGTCTC 3 cut(s) 1969, 3753, 4178
Eco32I GATATC 3 cut(s) 1633, 4578, 5462
Eco47I GGWCC 6 cut(s) 256, 518, 1936, 3775, 5031, 5248
Eco52I CGGCCG 1 cut(s) 4867
Eco81I CCTNAGG 1 cut(s) 522
Eco91I GGTNACC 1 cut(s) 936
EcoNI CCTNNNNNAGG 3 cut(s) 2419, 4328, 5788
EcoO109I RGGNCCY 3 cut(s) 526, 3775, 5628
EcoO65I GGTNACC 1 cut(s) 936
EcoRI GAATTC 2 cut(s) 3133, 5657
EcoRII CCWGG 5 cut(s) 65, 528, 3351, 3456, 3934
EcoRV GATATC 3 cut(s) 1633, 4578, 5462
EcoT14I CCWWGG 6 cut(s) 78, 2009, 2332, 3979, 4501, 4683
EcoT22I ATGCAT 2 cut(s) 3673, 3694
EcoT38I GRGCYC 2 cut(s) 27, 2242
ErhI CCWWGG 6 cut(s) 78, 2009, 2332, 3979, 4501, 4683
Esp3I CGTCTC 1 cut(s) 3247
FaqI GGGAC 4 cut(s) 715, 1237, 1922, 5153
FauNDI CATATG 4 cut(s) 1062, 1069, 1135, 5683
FbaI TGATCA 3 cut(s) 94, 589, 3907
FblI GTMKAC 2 cut(s) 2063, 5334
FriOI GRGCYC 2 cut(s) 27, 2242
GlaI GCGC 3 cut(s) 462, 2025, 3464
GsaI CCCAGC 2 cut(s) 1852, 4347
GsuI CTGGAG 3 cut(s) 3374, 3479, 3777
HaeIII GGCC 8 cut(s) 149, 527, 1306, 2629, 3540, 4869, 5629, 5783
HapII CCGG 5 cut(s) 160, 516, 934, 2613, 3535
HgaI GACGC 3 cut(s) 387, 1301, 3264
HhaI GCGC 3 cut(s) 463, 2026, 3465
Hin1I GRCGYC 1 cut(s) 4507
Hin6I GCGC 3 cut(s) 461, 2024, 3463
HinP1I GCGC 3 cut(s) 461, 2024, 3463
HincII GTYRAC 1 cut(s) 1089
HindII GTYRAC 1 cut(s) 1089
HindIII AAGCTT 2 cut(s) 1519, 3929
HpaI GTTAAC 1 cut(s) 1089
HpaII CCGG 5 cut(s) 160, 516, 934, 2613, 3535
Hpy99I CGWCG 2 cut(s) 264, 4368
HpyCH4IV ACGT 4 cut(s) 1495, 4379, 4507, 4835
HpySE526I ACGT 4 cut(s) 1495, 4379, 4507, 4835
Hsp92I GRCGYC 1 cut(s) 4507
HspAI GCGC 3 cut(s) 461, 2024, 3463
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 3 cut(s) 94, 589, 3907
KspAI GTTAAC 1 cut(s) 1089
LguI GCTCTTC 1 cut(s) 3504
MabI ACCWGGT 1 cut(s) 65
MaeII ACGT 4 cut(s) 1495, 4379, 4507, 4835
MaeIII GTNAC 7 cut(s) 936, 3721, 4195, 4463, 4487, 4992, 5062
MfeI CAATTG 1 cut(s) 4808
MflI RGATCY 6 cut(s) 685, 2800, 2899, 3116, 4431, 5802
MhlI GDGCHC 3 cut(s) 27, 2242, 3943
MlsI TGGCCA 1 cut(s) 2629
MluI ACGCGT 1 cut(s) 379
MluNI TGGCCA 1 cut(s) 2629
MlyI GAGTC 4 cut(s) 1160, 1334, 2319, 5755
MmeI TCCRAC 4 cut(s) 1370, 3257, 3471, 5830
Mox20I TGGCCA 1 cut(s) 2629
Mph1103I ATGCAT 2 cut(s) 3673, 3694
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 6 cut(s) 687, 3452, 3630, 4083, 4150, 4772
MscI TGGCCA 1 cut(s) 2629
MslI CAYNNNNRTG 4 cut(s) 2334, 4022, 4257, 4887
Msp20I TGGCCA 1 cut(s) 2629
MspA1I CMGCKG 2 cut(s) 1229, 4816
MspI CCGG 5 cut(s) 160, 516, 934, 2613, 3535
MspR9I CCNGG 6 cut(s) 67, 530, 3353, 3458, 3536, 3936
MunI CAATTG 1 cut(s) 4808
Mva1269I GAATGC 5 cut(s) 1372, 2390, 3385, 4743, 5753
MvaI CCWGG 5 cut(s) 67, 530, 3353, 3458, 3936
MvnI CGCG 4 cut(s) 369, 381, 463, 2026
NciI CCSGG 1 cut(s) 3536
NcoI CCATGG 2 cut(s) 78, 3979
NdeI CATATG 4 cut(s) 1062, 1069, 1135, 5683
NlaIV GGNNCC 8 cut(s) 201, 1938, 2241, 2522, 3324, 3539, 4775, 5630
NmeAIII GCCGAG 1 cut(s) 1231
NmuCI GTSAC 3 cut(s) 4195, 4487, 5062
NsiI ATGCAT 2 cut(s) 3673, 3694
NspI RCATGY 8 cut(s) 1087, 1581, 1748, 2737, 3302, 3424, 3770, 4396
NspV TTCGAA 2 cut(s) 266, 2344
OliI CACNNNNGTG 1 cut(s) 2334
PaeI GCATGC 1 cut(s) 1581
PagI TCATGA 3 cut(s) 1635, 2154, 4384
PceI AGGCCT 1 cut(s) 5783
PciI ACATGT 2 cut(s) 1744, 2733
PciSI GCTCTTC 1 cut(s) 3504
PcsI WCGNNNNNNNCGW 1 cut(s) 661
PctI GAATGC 5 cut(s) 1372, 2390, 3385, 4743, 5753
PdmI GAANNNNTTC 6 cut(s) 687, 3452, 3630, 4083, 4150, 4772
PflMI CCANNNNNTGG 4 cut(s) 1136, 1588, 3113, 3950
PfoI TCCNGGA 1 cut(s) 3456
PleI GAGTC 4 cut(s) 1159, 1334, 2318, 5755
PpsI GAGTC 4 cut(s) 1159, 1334, 2318, 5755
Ppu21I YACGTR 1 cut(s) 4380
PpuMI RGGWCCY 1 cut(s) 3775
PscI ACATGT 2 cut(s) 1744, 2733
PshAI GACNNNNGTC 1 cut(s) 833
PshBI ATTAAT 2 cut(s) 1565, 3363
PsiI TTATAA 2 cut(s) 1193, 5016
Psp5II RGGWCCY 1 cut(s) 3775
Psp6I CCWGG 5 cut(s) 65, 528, 3351, 3456, 3934
PspEI GGTNACC 1 cut(s) 936
PspFI CCCAGC 2 cut(s) 1848, 4343
PspGI CCWGG 5 cut(s) 65, 528, 3351, 3456, 3934
PspN4I GGNNCC 8 cut(s) 201, 1938, 2241, 2522, 3324, 3539, 4775, 5630
PspPPI RGGWCCY 1 cut(s) 3775
PsrI GAACNNNNNNTAC 2 cut(s) 457, 489
PstI CTGCAG 3 cut(s) 309, 859, 4671
PstNI CAGNNNCTG 2 cut(s) 529, 3077
PsuI RGATCY 6 cut(s) 685, 2800, 2899, 3116, 4431, 5802
PvuII CAGCTG 2 cut(s) 1229, 4816
RseI CAYNNNNRTG 4 cut(s) 2334, 4022, 4257, 4887
SapI GCTCTTC 1 cut(s) 3504
ScaI AGTACT 2 cut(s) 734, 5670
SchI GAGTC 4 cut(s) 1160, 1334, 2319, 5755
ScrFI CCNGG 6 cut(s) 67, 530, 3353, 3458, 3536, 3936
SduI GDGCHC 3 cut(s) 27, 2242, 3943
SexAI ACCWGGT 1 cut(s) 65
SfcI CTRYAG 7 cut(s) 305, 855, 1389, 1779, 3577, 4667, 4951
SfuI TTCGAA 2 cut(s) 266, 2344
SinI GGWCC 6 cut(s) 256, 518, 1936, 3775, 5031, 5248
SmiMI CAYNNNNRTG 4 cut(s) 2334, 4022, 4257, 4887
SmlI CTYRAG 1 cut(s) 4763
SmoI CTYRAG 1 cut(s) 4763
SphI GCATGC 1 cut(s) 1581
SseBI AGGCCT 1 cut(s) 5783
SsiI CCGC 4 cut(s) 223, 638, 874, 4498
SspI AATATT 3 cut(s) 577, 802, 2398
StuI AGGCCT 1 cut(s) 5783
StyD4I CCNGG 6 cut(s) 65, 528, 3351, 3456, 3534, 3934
StyI CCWWGG 6 cut(s) 78, 2009, 2332, 3979, 4501, 4683
TaiI ACGT 4 cut(s) 1498, 4382, 4510, 4838
TatI WGTACW 9 cut(s) 402, 732, 2083, 2256, 2730, 2736, 2772, 3573, 5668
TauI GCSGC 2 cut(s) 225, 641
TseFI GTSAC 3 cut(s) 4195, 4487, 5062
Tsp45I GTSAC 3 cut(s) 4195, 4487, 5062
TspGWI ACGGA 8 cut(s) 361, 826, 1442, 1857, 3800, 4623, 5112, 5706
Van91I CCANNNNNTGG 4 cut(s) 1136, 1588, 3113, 3950
VneI GTGCAC 1 cut(s) 3939
VpaK11BI GGWCC 6 cut(s) 256, 518, 1936, 3775, 5031, 5248
VspI ATTAAT 2 cut(s) 1565, 3363
XagI CCTNNNNNAGG 3 cut(s) 2419, 4328, 5788
XbaI TCTAGA 2 cut(s) 1315, 1808
XceI RCATGY 8 cut(s) 1087, 1581, 1748, 2737, 3302, 3424, 3770, 4396
XcmI CCANNNNNNNNNTGG 2 cut(s) 2178, 2509
XmaJI CCTAGG 1 cut(s) 2009
XmiI GTMKAC 2 cut(s) 2063, 5334
XmnI GAANNNNTTC 6 cut(s) 687, 3452, 3630, 4083, 4150, 4772
ZraI GACGTC 1 cut(s) 4508
ZrmI AGTACT 2 cut(s) 734, 5670
Zsp2I ATGCAT 2 cut(s) 3673, 3694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.