Rorug05G0105400

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016003
Physical Location & Seq
Forward (+)
9292055 .. 9295457
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0105400.1

Sequence Viewer

Length: 2136 bp
ATGCCATCCCAATCATATTGGACTGATATGGGAAAGGGCACCGCACACTCCCTCCCTCGCTTCATCTTCTCACTGCGACCTTTCGTCGAGCACCTGGTGCGCACCTCCCATTTTCCCGGACCTTCAACTTTCGCTCGCGCTCTCTCTTCTTCCCTCACCGCCCCACCTCCCCTGAAAGACCAGCAAGACCCCTACGCCCTCCTCAAACAAGACCCAATTGAAATCTGCTCCAATCTCTGGGTCAAAGCCTTCTCCTCCCCACCAACCACGACCTTCCCCAACCTCACCGGCTTCCTCTCCAAATTCGACCTCTGGGTCCTCGCCTACCAGCGCTGCTGCGCCCACGTGACCGGCACGTTCCCTCCCCGCAACGCCGTCCACTCCCACGTCCTCCACGACCTCCTCTCCCTCCGAAACGCCGTCGTTAAAGGAAACTATGCCTGGAACAAGAAGACCCACCAGTTCATTCGGAGCCCCAACGACAGGCCCAGCACCGAGGTCGTCTCCAAGCGGAAGCTCCAGGCAATGCTCGAGTCCGACGAGCCTTGCTTTCAGGATAGGATTGTGCAGGAGGTGTTGCTGATGATCATTGAGCCGGTTTTCGAGGCCCGATTCTCGCCGAAAGCCCATGGGTTTCGGCCCGGCCGGAATGCCCACACTGTAATTCGGACCATCCGGAGCAACTTCGCCGGGTACTTGTGGTTTCTGAAGGGAGATGTCAGTGAGGTTTTCGAAAATGTGGATGGAAATGTGGTGATGGGTTGCTTGGAGCAGGCAGTGAGGGATAGGAAGATATTGGGTTTGGTGAAATCTGCACTCAGAGCACCGAATCGGATACAGAGCAGTGATGGTGATGAACAATGGCCGAGGAAGAAGAAGAAGAAGAAGGTTTCAAAGAAGAAGAAGATTTTGAATGACAAGGAAGCGAAACCGGACCCGTATTGGCTCAGAACCTTTTTCAACTTTGCACCTGAGGAAGCAGCTAAGGTACCTTGTTATGGGAACTGTGGAATTTTGAGTCCTTTACTTTCGAATGTGTGTCTGAATGAGTTGGATCAAGTAATGGAAGAGAAGATAGTTGAGTTTTTTAGGCCATCTGAGACTGATTCTATATGGAAAAATTCGATTAATGATGGCTGTCATAACCCGGCTTGGCCGGAGTTTGTGCCTGCGAGTGGGGATGAGAAAACAAGGAAAATGGATTACATTAGGTATGGGGGTCATTTCTTGATTGGTATTCGAGGGCCTAGAGAGGATGCAGTGGATATGCGAAAGCAAATCATTGAGTTTTGTGAGAGAAGATTCGGGTTAAGGTTGGATAATGCTAAATTGGAGATTGAACACATAACTAGGGGGATTCAGTTTTTGGACCATATAATTTGCAGGAGGGTTATACACCCTACACTTAGGTACACAGCCACTGGTGGTAATGTTGTGAGTGAAAAAGGTGTAGGGACTTTGCTTTCAGTTACTGCTAGCTTACAACAATGTATTCGCCAATTCCGGCGGCTTGCATTTGTTAAGGGTGATAAAGATCCTGAGCCATTGCCCTGCACTCCGATGCTTTACTCGAGTCAAGCTCATACTAACTCTCAGATGAATAAGTTATTGGAGACCATGGCTGATTGGTACAGATACGCTGATAATCGCAAGAAAGTTGTTGGTTTTTGTGCTTATGTGATTCGCAGCTCTTTGGCTAAACTGTATGCTGCAAGGTATAGACTAAAATCAAGGGCCAAAGTGTATAGTATAGCTACCCGCGATCTCAGTCGTCCACTGAGGGAGAGCAGTAACAACTCTGCACCTGAATATTCTGATCTATTAAGGATGGGACTTGTTGATGCAATTGAAGGTGTTCGGTTCTCGCACATGTCTTTGATCCCATCTTGTGATTACTCGCCATTTCCTAGGAATTGGGTTCCAGATCATGAGCGGCTCTTACGAGACTACATCAGACTTGAAGACCCAAAGTTCTTCTGTGCCTTACATAGATCAATTAAAAAAGATGGTTTAAGTTTGCCTCAAGATGAGATATCTGAACTTCTGTGGCATTTCAAGTCCGTTGGGGTTCGGAACTATCTGCTAAAGGAGAAAAGGAAACAAAAACATGATGTCGTGGACATTCGTGGTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

711

Amino Acids

81.55

Weight (kDa)

9.46

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
Acc16I TGCGCA 1 cut(s) 101
Acc65I GGTACC 1 cut(s) 988
AccB1I GGYRCC 2 cut(s) 38, 988
AccB7I CCANNNNNTGG 1 cut(s) 237
AccBSI CCGCTC 1 cut(s) 1933
AccII CGCG 2 cut(s) 138, 1761
AccIII TCCGGA 1 cut(s) 675
AciI CCGC 7 cut(s) 42, 159, 367, 511, 1507, 1759, 1933
AclWI GGATC 3 cut(s) 1062, 1529, 1873
AcoI YGGCCR 3 cut(s) 643, 863, 1154
AcsI RAATTY 3 cut(s) 302, 1011, 1120
AcuI CTGAAG 1 cut(s) 728
AcvI CACGTG 1 cut(s) 346
AdeI CACNNNGTG 1 cut(s) 97
AfaI GTAC 5 cut(s) 695, 990, 1412, 1631, 2131
AfeI AGCGCT 1 cut(s) 332
AfiI CCNNNNNNNGG 4 cut(s) 237, 483, 998, 1175
AflIII ACRYGT 1 cut(s) 1869
AgsI TTSAA 9 cut(s) 126, 221, 894, 913, 961, 1340, 1850, 1961, 2056
AhdI GACNNNNNGTC 1 cut(s) 83
AjiI CACGTC 1 cut(s) 388
AjnI CCWGG 3 cut(s) 93, 440, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 1592, 1624
AluBI AGCT 6 cut(s) 517, 983, 1479, 1580, 1689, 1754
AluI AGCT 6 cut(s) 517, 983, 1479, 1580, 1689, 1754
Alw21I GWGCWC 2 cut(s) 93, 826
Alw26I GTCTC 4 cut(s) 508, 1094, 1607, 1938
AlwI GGATC 3 cut(s) 1062, 1529, 1873
AlwNI CAGNNNCTG 2 cut(s) 1421, 1472
Ama87I CYCGRG 2 cut(s) 530, 1570
Aor13HI TCCGGA 1 cut(s) 675
Aor51HI AGCGCT 1 cut(s) 332
AoxI GGCC 9 cut(s) 485, 606, 638, 643, 863, 1091, 1154, 1244, 1734
ApeKI GCWGC 5 cut(s) 333, 336, 980, 1686, 1709
ApoI RAATTY 3 cut(s) 302, 1011, 1120
AseI ATTAAT 1 cut(s) 1128
Asp700I GAANNNNTTC 1 cut(s) 1854
Asp718I GGTACC 1 cut(s) 988
AspA2I CCTAGG 1 cut(s) 1907
AspLEI GCGC 4 cut(s) 102, 140, 333, 341
AsuC2I CCSGG 4 cut(s) 117, 642, 691, 1148
AsuHPI GGTGA 6 cut(s) 148, 277, 766, 817, 863, 1538
AsuII TTCGAA 2 cut(s) 732, 1031
AsuNHI GCTAGC 1 cut(s) 1475
AvaI CYCGRG 2 cut(s) 530, 1570
AvaII GGWCC 5 cut(s) 119, 316, 669, 934, 1369
AvrII CCTAGG 1 cut(s) 1907
AxyI CCTNAGG 1 cut(s) 972
BaeGI GKGCMC 1 cut(s) 41
BanI GGYRCC 2 cut(s) 38, 988
BanII GRGCYC 1 cut(s) 476
BbrPI CACGTG 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 458, 1968
Bbv12I GWGCWC 2 cut(s) 93, 826
BbvI GCAGC 5 cut(s) 320, 323, 992, 1696, 1698
BceAI ACGGC 2 cut(s) 359, 404
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 3 cut(s) 95, 442, 521
BciVI GTATCC 1 cut(s) 828
BclI TGATCA 1 cut(s) 585
BcnI CCSGG 4 cut(s) 117, 642, 691, 1148
BcoDI GTCTC 4 cut(s) 508, 1094, 1607, 1938
BfaI CTAG 4 cut(s) 1248, 1350, 1476, 1908
BfoI RGCGCY 1 cut(s) 334
BfuI GTATCC 1 cut(s) 828
BisI GCNGC 7 cut(s) 334, 337, 981, 1508, 1687, 1710, 1934
BlnI CCTAGG 1 cut(s) 1907
BlsI GCNGC 7 cut(s) 335, 338, 982, 1509, 1688, 1711, 1935
Bme1390I CCNGG 7 cut(s) 95, 117, 442, 521, 642, 691, 1148
Bme18I GGWCC 5 cut(s) 119, 316, 669, 934, 1369
BmeRI GACNNNNNGTC 1 cut(s) 83
BmeT110I CYCGRG 2 cut(s) 530, 1570
BmgBI CACGTC 1 cut(s) 388
BmiI GGNNCC 6 cut(s) 40, 317, 473, 936, 990, 1920
BmrFI CCNGG 7 cut(s) 95, 117, 442, 521, 642, 691, 1148
BmsI GCATC 3 cut(s) 1246, 1551, 1831
BmtI GCTAGC 1 cut(s) 1479
BpiI GAAGAC 2 cut(s) 458, 1968
BplI GAGNNNNNCTC 4 cut(s) 488, 520, 1564, 1596
BpmI CTGGAG 1 cut(s) 503
Bpu10I CCTNAGC 2 cut(s) 984, 1539
Bpu14I TTCGAA 2 cut(s) 732, 1031
BpuEI CTTGAG 1 cut(s) 2007
BpuMI CCSGG 4 cut(s) 117, 642, 691, 1148
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 1533
BsaI GGTCTC 1 cut(s) 1607
BsaJI CCNNGG 5 cut(s) 495, 628, 866, 1617, 1907
BsaWI WCCGGW 2 cut(s) 675, 931
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 346, 376, 389, 419
Bsc4I CCNNNNNNNGG 4 cut(s) 237, 483, 998, 1175
Bse118I RCCGGY 3 cut(s) 287, 350, 595
Bse1I ACTGG 2 cut(s) 460, 1426
Bse21I CCTNAGG 1 cut(s) 972
Bse3DI GCAATG 2 cut(s) 531, 1544
Bse8I GATNNNNATC 1 cut(s) 1533
BseAI TCCGGA 1 cut(s) 675
BseBI CCWGG 3 cut(s) 95, 442, 521
BseDI CCNNGG 5 cut(s) 495, 628, 866, 1617, 1907
BseGI GGATG 6 cut(s) 5, 672, 748, 1186, 1261, 1833
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 4 cut(s) 237, 483, 998, 1175
BseMI GCAATG 2 cut(s) 531, 1544
BseMII CTCAG 8 cut(s) 832, 961, 963, 1089, 1530, 1607, 1769, 1780
BseNI ACTGG 2 cut(s) 460, 1426
BseRI GAGGAG 3 cut(s) 191, 244, 392
BseSI GKGCMC 1 cut(s) 41
BseX3I CGGCCG 1 cut(s) 643
BseXI GCAGC 5 cut(s) 320, 323, 992, 1696, 1698
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 4 cut(s) 587, 798, 1537, 1785
Bsh1236I CGCG 2 cut(s) 138, 1761
Bsh1285I CGRYCG 1 cut(s) 646
BshFI GGCC 9 cut(s) 487, 608, 640, 645, 865, 1093, 1156, 1246, 1736
BshNI GGYRCC 2 cut(s) 38, 988
BsiEI CGRYCG 1 cut(s) 646
BsiHKAI GWGCWC 2 cut(s) 93, 826
BsiHKCI CYCGRG 2 cut(s) 530, 1570
BslFI GGGAC 2 cut(s) 1468, 1845
BslI CCNNNNNNNGG 4 cut(s) 237, 483, 998, 1175
BsmAI GTCTC 4 cut(s) 508, 1094, 1607, 1938
BsmBI CGTCTC 1 cut(s) 508
BsmFI GGGAC 2 cut(s) 1468, 1845
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 9 cut(s) 487, 608, 640, 645, 865, 1093, 1156, 1246, 1736
Bso31I GGTCTC 1 cut(s) 1607
BsoBI CYCGRG 2 cut(s) 530, 1570
Bsp119I TTCGAA 2 cut(s) 732, 1031
Bsp1286I GDGCHC 4 cut(s) 41, 93, 476, 826
Bsp13I TCCGGA 1 cut(s) 675
Bsp143I GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
Bsp19I CCATGG 2 cut(s) 628, 1617
BspACI CCGC 7 cut(s) 42, 159, 367, 511, 1507, 1759, 1933
BspANI GGCC 9 cut(s) 487, 608, 640, 645, 865, 1093, 1156, 1246, 1736
BspCNI CTCAG 8 cut(s) 831, 960, 964, 1090, 1531, 1606, 1770, 1779
BspEI TCCGGA 1 cut(s) 675
BspFNI CGCG 2 cut(s) 138, 1761
BspHI TCATGA 1 cut(s) 1927
BspLI GGNNCC 6 cut(s) 40, 317, 473, 936, 990, 1920
BspOI GCTAGC 1 cut(s) 1479
BspPI GGATC 3 cut(s) 1062, 1529, 1873
BspT104I TTCGAA 2 cut(s) 732, 1031
BspT107I GGYRCC 2 cut(s) 38, 988
BspTNI GGTCTC 1 cut(s) 1607
BsrBI CCGCTC 1 cut(s) 1933
BsrDI GCAATG 2 cut(s) 531, 1544
BsrFI RCCGGY 3 cut(s) 287, 350, 595
BsrI ACTGG 2 cut(s) 460, 1426
BssAI RCCGGY 3 cut(s) 287, 350, 595
BssECI CCNNGG 5 cut(s) 495, 628, 866, 1617, 1907
BssMI GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
BssT1I CCWWGG 3 cut(s) 628, 1617, 1907
Bst2UI CCWGG 3 cut(s) 95, 442, 521
Bst4CI ACNGT 3 cut(s) 661, 1007, 1704
Bst6I CTCTTC 2 cut(s) 151, 1062
BstAPI GCANNNNNTGC 1 cut(s) 97
BstBAI YACGTR 1 cut(s) 346
BstBI TTCGAA 2 cut(s) 732, 1031
BstC8I GCNNGC 5 cut(s) 136, 774, 1170, 1477, 1512
BstDSI CCRYGG 2 cut(s) 628, 1617
BstF5I GGATG 6 cut(s) 5, 672, 748, 1186, 1261, 1833
BstFNI CGCG 2 cut(s) 138, 1761
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 4 cut(s) 102, 140, 333, 341
BstKTI GATC 8 cut(s) 588, 1057, 1537, 1765, 1819, 1881, 1927, 1994
BstMAI GTCTC 4 cut(s) 508, 1094, 1607, 1938
BstMBI GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
BstMCI CGRYCG 1 cut(s) 646
BstMWI GCNNNNNNNGC 2 cut(s) 97, 821
BstNI CCWGG 3 cut(s) 95, 442, 521
BstNSI RCATGY 1 cut(s) 1873
BstSCI CCNGG 7 cut(s) 93, 115, 440, 519, 640, 689, 1146
BstSLI GKGCMC 1 cut(s) 41
BstUI CGCG 2 cut(s) 138, 1761
BstV1I GCAGC 5 cut(s) 320, 323, 992, 1696, 1698
BstV2I GAAGAC 2 cut(s) 458, 1968
BstX2I RGATCY 1 cut(s) 1534
BstYI RGATCY 1 cut(s) 1534
BstZI CGGCCG 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 972
BsuI GTATCC 1 cut(s) 828
BsuRI GGCC 9 cut(s) 487, 608, 640, 645, 865, 1093, 1156, 1246, 1736
BtgI CCRYGG 2 cut(s) 628, 1617
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 6 cut(s) 5, 672, 748, 1186, 1261, 1833
BtsI GCAGTG 4 cut(s) 71, 783, 850, 1266
BtsIMutI CAGTG 8 cut(s) 71, 657, 727, 783, 850, 1266, 1419, 1775
Cac8I GCNNGC 5 cut(s) 136, 774, 1170, 1477, 1512
CaiI CAGNNNCTG 2 cut(s) 1421, 1472
CciI TCATGA 1 cut(s) 1927
CfoI GCGC 4 cut(s) 102, 140, 333, 341
Cfr10I RCCGGY 3 cut(s) 287, 350, 595
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 5 cut(s) 694, 989, 1411, 1630, 2130
CviAII CATG 6 cut(s) 629, 1618, 1870, 1928, 2108, 2133
CviQI GTAC 5 cut(s) 694, 989, 1411, 1630, 2130
DpnI GATC 8 cut(s) 587, 1056, 1536, 1764, 1818, 1880, 1926, 1993
DpnII GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
DraIII CACNNNGTG 1 cut(s) 97
DrdI GACNNNNNNGTC 1 cut(s) 314
DriI GACNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 314
EaeI YGGCCR 3 cut(s) 643, 863, 1154
EagI CGGCCG 1 cut(s) 643
Eam1104I CTCTTC 2 cut(s) 151, 1062
Eam1105I GACNNNNNGTC 1 cut(s) 83
EarI CTCTTC 2 cut(s) 151, 1062
EclXI CGGCCG 1 cut(s) 643
Eco130I CCWWGG 3 cut(s) 628, 1617, 1907
Eco24I GRGCYC 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 1607
Eco32I GATATC 1 cut(s) 2034
Eco47I GGWCC 5 cut(s) 119, 316, 669, 934, 1369
Eco47III AGCGCT 1 cut(s) 332
Eco52I CGGCCG 1 cut(s) 643
Eco57I CTGAAG 1 cut(s) 728
Eco72I CACGTG 1 cut(s) 346
Eco81I CCTNAGG 1 cut(s) 972
Eco88I CYCGRG 2 cut(s) 530, 1570
EcoO109I RGGNCCY 2 cut(s) 316, 1244
EcoRII CCWGG 3 cut(s) 93, 440, 519
EcoRV GATATC 1 cut(s) 2034
EcoT14I CCWWGG 3 cut(s) 628, 1617, 1907
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 3 cut(s) 628, 1617, 1907
Esp3I CGTCTC 1 cut(s) 508
FaeI CATG 6 cut(s) 632, 1621, 1873, 1931, 2111, 2136
FaqI GGGAC 2 cut(s) 1468, 1845
FatI CATG 6 cut(s) 628, 1617, 1869, 1927, 2107, 2132
FauI CCCGC 2 cut(s) 374, 1766
FbaI TGATCA 1 cut(s) 585
Fnu4HI GCNGC 7 cut(s) 334, 337, 981, 1508, 1687, 1710, 1934
FokI GGATG 5 cut(s) 659, 755, 1193, 1268, 1840
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 7 cut(s) 334, 337, 981, 1508, 1687, 1710, 1934
FspAI RTGCGCAY 1 cut(s) 101
FspBI CTAG 4 cut(s) 1248, 1350, 1476, 1908
FspI TGCGCA 1 cut(s) 101
GlaI GCGC 4 cut(s) 101, 139, 332, 340
GluI GCNGC 7 cut(s) 334, 337, 981, 1508, 1687, 1710, 1934
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 1 cut(s) 503
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 9 cut(s) 487, 608, 640, 645, 865, 1093, 1156, 1246, 1736
HhaI GCGC 4 cut(s) 102, 140, 333, 341
Hin1II CATG 6 cut(s) 632, 1621, 1873, 1931, 2111, 2136
Hin6I GCGC 4 cut(s) 100, 138, 331, 339
HinP1I GCGC 4 cut(s) 100, 138, 331, 339
HinfI GANTC 9 cut(s) 533, 612, 829, 1018, 1106, 1302, 1357, 1573, 1681
HphI GGTGA 6 cut(s) 148, 277, 766, 817, 863, 1538
Hpy166II GTNNAC 4 cut(s) 379, 1413, 1775, 2119
Hpy188III TCNNGA 7 cut(s) 554, 676, 1228, 1538, 1922, 1928, 2024
Hpy8I GTNNAC 4 cut(s) 379, 1413, 1775, 2119
Hpy99I CGWCG 3 cut(s) 89, 425, 542
HpyAV CCTTC 6 cut(s) 132, 259, 283, 703, 880, 1844
HpyCH4III ACNGT 3 cut(s) 661, 1007, 1704
HpyCH4IV ACGT 3 cut(s) 345, 356, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 821
HpySE526I ACGT 3 cut(s) 345, 356, 387
Hsp92II CATG 6 cut(s) 632, 1621, 1873, 1931, 2111, 2136
HspAI GCGC 4 cut(s) 100, 138, 331, 339
Kpn2I TCCGGA 1 cut(s) 675
KpnI GGTACC 1 cut(s) 992
Ksp22I TGATCA 1 cut(s) 585
Kzo9I GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
LmnI GCTCC 5 cut(s) 233, 471, 522, 678, 769
Lsp1109I GCAGC 5 cut(s) 320, 323, 992, 1696, 1698
LweI GCATC 3 cut(s) 1246, 1551, 1831
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 4 cut(s) 1248, 1350, 1476, 1908
MaeII ACGT 3 cut(s) 345, 356, 387
MaeIII GTNAC 3 cut(s) 346, 1468, 1790
MalI GATC 8 cut(s) 587, 1056, 1536, 1764, 1818, 1880, 1926, 1993
MbiI CCGCTC 1 cut(s) 1933
MboI GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
MfeI CAATTG 2 cut(s) 216, 1845
MflI RGATCY 1 cut(s) 1534
MhlI GDGCHC 4 cut(s) 41, 93, 476, 826
MlyI GAGTC 3 cut(s) 542, 1027, 1582
MmeI TCCRAC 3 cut(s) 561, 1032, 1296
MroI TCCGGA 1 cut(s) 675
MroXI GAANNNNTTC 1 cut(s) 1854
MseI TTAA 7 cut(s) 426, 1128, 1310, 1521, 1823, 1998, 2012
MslI CAYNNNNRTG 1 cut(s) 1559
MspR9I CCNGG 7 cut(s) 95, 117, 442, 521, 642, 691, 1148
MunI CAATTG 2 cut(s) 216, 1845
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 3 cut(s) 95, 442, 521
MvnI CGCG 2 cut(s) 138, 1761
MwoI GCNNNNNNNGC 2 cut(s) 97, 821
NciI CCSGG 4 cut(s) 117, 642, 691, 1148
NcoI CCATGG 2 cut(s) 628, 1617
NdeII GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
NheI GCTAGC 1 cut(s) 1475
NlaIII CATG 6 cut(s) 632, 1621, 1873, 1931, 2111, 2136
NlaIV GGNNCC 6 cut(s) 40, 317, 473, 936, 990, 1920
NmeAIII GCCGAG 1 cut(s) 891
NmuCI GTSAC 1 cut(s) 346
NsbI TGCGCA 1 cut(s) 101
NspI RCATGY 1 cut(s) 1873
NspV TTCGAA 2 cut(s) 732, 1031
PaeR7I CTCGAG 2 cut(s) 530, 1570
PagI TCATGA 1 cut(s) 1927
PciI ACATGT 1 cut(s) 1869
PcsI WCGNNNNNNNCGW 2 cut(s) 393, 537
PctI GAATGC 1 cut(s) 655
PdmI GAANNNNTTC 1 cut(s) 1854
PfeI GAWTC 6 cut(s) 612, 829, 1106, 1302, 1357, 1681
PflMI CCANNNNNTGG 1 cut(s) 237
PfoI TCCNGGA 1 cut(s) 115
PkrI GCNGC 7 cut(s) 335, 338, 982, 1509, 1688, 1711, 1935
PleI GAGTC 3 cut(s) 541, 1026, 1581
PmaCI CACGTG 1 cut(s) 346
PmlI CACGTG 1 cut(s) 346
PpsI GAGTC 3 cut(s) 541, 1026, 1581
Ppu21I YACGTR 1 cut(s) 346
PpuMI RGGWCCY 1 cut(s) 316
PscI ACATGT 1 cut(s) 1869
PshBI ATTAAT 1 cut(s) 1128
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 3 cut(s) 93, 440, 519
PspCI CACGTG 1 cut(s) 346
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 3 cut(s) 93, 440, 519
PspN4I GGNNCC 6 cut(s) 40, 317, 473, 936, 990, 1920
PspPPI RGGWCCY 1 cut(s) 316
PspXI VCTCGAGB 2 cut(s) 530, 1570
PstNI CAGNNNCTG 2 cut(s) 1421, 1472
PsuI RGATCY 1 cut(s) 1534
RsaI GTAC 5 cut(s) 695, 990, 1412, 1631, 2131
RsaNI GTAC 5 cut(s) 694, 989, 1411, 1630, 2130
RseI CAYNNNNRTG 1 cut(s) 1559
SaqAI TTAA 7 cut(s) 426, 1128, 1310, 1521, 1823, 1998, 2012
SatI GCNGC 7 cut(s) 334, 337, 981, 1508, 1687, 1710, 1934
Sau3AI GATC 8 cut(s) 585, 1054, 1534, 1762, 1816, 1878, 1924, 1991
SchI GAGTC 3 cut(s) 542, 1027, 1582
ScrFI CCNGG 7 cut(s) 95, 117, 442, 521, 642, 691, 1148
SduI GDGCHC 4 cut(s) 41, 93, 476, 826
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 3 cut(s) 1246, 1551, 1831
Sfr274I CTCGAG 2 cut(s) 530, 1570
SfuI TTCGAA 2 cut(s) 732, 1031
SinI GGWCC 5 cut(s) 119, 316, 669, 934, 1369
SlaI CTCGAG 2 cut(s) 530, 1570
SmiMI CAYNNNNRTG 1 cut(s) 1559
SmlI CTYRAG 3 cut(s) 530, 1570, 2022
SmoI CTYRAG 3 cut(s) 530, 1570, 2022
SsiI CCGC 7 cut(s) 42, 159, 367, 511, 1507, 1759, 1933
SspI AATATT 1 cut(s) 1811
SspMI CTAG 4 cut(s) 1248, 1350, 1476, 1908
StyD4I CCNGG 7 cut(s) 93, 115, 440, 519, 640, 689, 1146
StyI CCWWGG 3 cut(s) 628, 1617, 1907
TaaI ACNGT 3 cut(s) 661, 1007, 1704
TaiI ACGT 3 cut(s) 348, 359, 390
TaqI TCGA 9 cut(s) 87, 306, 531, 603, 732, 1031, 1124, 1240, 1571
TauI GCSGC 2 cut(s) 1510, 1936
TfiI GAWTC 6 cut(s) 612, 829, 1106, 1302, 1357, 1681
Tru1I TTAA 7 cut(s) 426, 1128, 1310, 1521, 1823, 1998, 2012
Tru9I TTAA 7 cut(s) 426, 1128, 1310, 1521, 1823, 1998, 2012
TscAI CASTG 8 cut(s) 78, 664, 727, 783, 850, 1266, 1426, 1782
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 5 cut(s) 333, 336, 980, 1686, 1709
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 4 cut(s) 52, 454, 870, 1613
TspGWI ACGGA 1 cut(s) 2050
TspRI CASTG 8 cut(s) 78, 664, 727, 783, 850, 1266, 1426, 1782
Van91I CCANNNNNTGG 1 cut(s) 237
VpaK11BI GGWCC 5 cut(s) 119, 316, 669, 934, 1369
VspI ATTAAT 1 cut(s) 1128
XapI RAATTY 3 cut(s) 302, 1011, 1120
XceI RCATGY 1 cut(s) 1873
XhoI CTCGAG 2 cut(s) 530, 1570
XmaJI CCTAGG 1 cut(s) 1907
XmnI GAANNNNTTC 1 cut(s) 1854
XspI CTAG 4 cut(s) 1248, 1350, 1476, 1908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.