Rroxscaffold_1G00051450

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016003
Physical Location & Seq
Reverse (-)
72063236 .. 72084119
20884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051450.1

Sequence Viewer

Length: 5844 bp
ATGTCTGAGATTGTGGTTGCAGAGCCCATCTATGATGGTCGAGCAGAGGAAAAAAGACCCATTACAAGGTATCTCACCATGGGCAGTGGGAGTTATCATAATCATCCTCCTGAGCCATTTGAGAGCGAGAGATTGCCTGCCACTTTGGCCTCAAAAATCCGGAGCTTTCTTCGGGTTGCAAACTTGATCGAAAGCGAGCAGCCTCGGATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCATTCGAATGGGAGGGGTGTTCGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACAATTAGGAAACGGAAGGAAAAGAGTGATATACGCGAGTTAAGACGCGTTTATCATGCTTACAAGGAGTACATCATCAAACATGAAGGAGCATTTAGCATAGAAAATAGTCACAGAGAGAAGTTGATAAATGCACGTATAATTGGTTCTGTATTGTTTGAAGTCTTAAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTGGCTAATAGAGGTGGTATCCAGACAAAACCTTATGATATCTTTGGCATGCCATATAATATTCTACCACTTGATCAAGGAGGTATTCAGCAACCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAGTACGGTGTATTCGTGGCATACCTTCCAATGAAGATCTTCAGAAACATGGGACTTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTCTACTCCTTGCTAATATTCACAAACGGAAAACTCATAAGCAGACATCTGTCTCAAAATTAGGAGATGCTGCAGTGGACGAGTTAATGCGGAGAATTTTTAAAAATTATACAAACTGGTGCAAATTTTTGGGAAGGAAAAGCAACATCCGGTTACCTTATGTGAAACAAGAAGCCCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGCGAGGCCGCAAACTTGCGATTCATGCCAGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGGGCTGTTAGCTTGACAACATGGGAGAAGGTCATGCCAGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTTGTTACCCCCATATATTGGGTTATAAGAGAGGAAGCCGAGAAAAGCAAGTGTGGGACAGCTGACCATTCCACATGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTCCTGATTGTTTTGAAATCGGTTGGCCCATGCGTCTAGATCACAATTTCTTCTTTACAGACTCTCCAACAAAATCCAATGCTAAGAATGCTTCAGCATCCACTTCTCCTGTAGAAGAAAGAAGGAAGGAAGATGAAGTTAGGGTTACTAAAGAAGAAGTCTGTGAACCCAAATGGTTGGGTAAAACAAATTTCGTAGAAGTACGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCACTTCAGGCATTAATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAGTTGTTTGACAAAGTTATATTTGAGGATATCATGAGCATCTTCATTACATCTGCATTTCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACACTGGACTTCTCTGCAAAAGTAAAACATGTTATGAAGCTCGGTGTTGCAATGATATGGTCTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATCCCACAACATATGGAAGCTGGGTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATTTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTATTCGCAAATACATTGAGGTCTCAAATTACCGAATATGCACCATTCTGTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGATTGCAGGAAAGCCAGCTGTCAGTGTTGAAGTATACATTGTTTTGGGTACTTGTACTATTGAGCAAATTTTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCAAATGTATGATTGGCATGAGCTATTTCCAAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTTTATGGCATTTTGCATCACCTTGGTGAGATTCGGACATTGGGAATGTTGAGGAGTAGATTTTACTCCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATTGAGAAATGACCAAAAAAGAAGAAAAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGACTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCTGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAAACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAAGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTGAGGACTTTAGGATGATTAAAGTTCCAGATCTTTTGTCTAAATGTATCGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCATGGTAAAGTTGTGAAACTTCTCCAAGATATATTTGAGCTTGTAACCAATGATATGATGACGACTGGCTTCAGGATATTAGAGTTGCTGGATTCATCCCAACAGACCGAAACAGATGCTGCTTATTTTTCTGGAAGCATTGAATCCCCATTATTTGGATCTGCTGGTGGCAAGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTGAATGAGCAGATCAAGCGTTTTCTTCTGTTGCTTACTGTCCAAGATACAGCAATGGACATTCCAACTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGCATATGCCACATGCACCCACAGTGGCCAATATGGTGCCATTCAGTGTTATGACACCACATTACCTGGAGGATATTAATTTTTCAATGGAGGAGCTTCATTCAAGCCAGCGAGAAGTTTCTATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCCTGGAGCGCATGGGTTATGAAAATTTGGATGGGTTGGAAAAGGACAAAAAGGAAGAGCTTAGAAATTGGGCTTCTTTCCGGGGCCAAACACTGAGCAGAACAGTTAGAGGAATGATGTACTATAGAGAAGCCTTAAAACTACAAGCATTTCTTGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATGCATTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTTGTTACCTGCCAATTATTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAAAAGATACCCAACTCTCCGTGTTGCTTACGTTGAGGAGAAAGAAGATATAGTGGAGAACAAGCCTTGCAAAGTTTATTCTTCAGTATTGGTTAAAGCTATTCCTGATTTTGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTGCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAACCATGGAATAATCTTCACTCGTGGTGAAGCTCTCCAAACCATTGATATGAACCAAGACAGTTATTTGGAGGAAGCATTCAAAATGAGAAATCTTCTGCAAGAATTTCTTCAGAACCGAGGACGGCGACCTCCTATACTGCTTGGTTTGAGGGAACACATATTTACAGGAAGTGTTTCTTCTCTCGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTAAGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGATTAAGCAAAGCATCAAAAACAATTAACCTGAGCGAGGATGTTTTTGCTGGGTATAATTCTACCTTACGTCGGGGATGGATCACGTATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAGTTTGAAGCTAAGGTTGCTAATGGTAACAGTGAACAAACTATAAGCCGTGACATATACCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTACGGACAGCTATACCTAGTTCTCAGTGGCTTGGAAAGAGCGCTCGTAATTGAGGCTAGACTCCAGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGCTATTAACAGGAATGCCAATGGTGATGGAAATTGGACTTGAGAAAGGGTTCCTCAATGCACTAAAAGATTTTGTCCTGATGCAATTGCAGCTGGCTTCTGTGTTTTTCACGTTCTCCTTTGGAACAAAAATCCATTATTACGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTTGTTGTGTTTCATACTAGCTTTACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTCATCAAAGGATTTGAGTTACTGCTCCTGTTGATTGTTTATGACTTGTTTAGAAGGTCCTATGAGAGCAGCATGGCATATGTATTAATCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTCGCTCCATTTCTTTTTAATCCATCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGATTGGAATAAGTGGATTAGGCAGCAGGGTGGTATTGGAGTTCACCAAGATAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGAGTTCTAGGTTGTTTGAAATACTCCTTTCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCAGGAATTTTCTGGTTTATTTGCTTTCATGGATAGTGATCCTCGTTGTTTTCTTGTTAGTAAAGGCAGTGAACCTTGGAAGGCAACAGTTCAGTGTTAGACATCACCTTATGTTTAGGCTATTCAAAGCAATCCTTTTCTTAGGTGTTCTTTCCATCATAATATCTCTCTCTGTTGTGTGTCATCTATCTTGGATGGACTTGGTTGTGTGCTGCCTAGCATTTTTGCCAACAGGGTGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAGTACTGGATTGTGGGAATTCACCAGAGTACTTGCTAAAACATATGACTATGGAATGGGGGTTGTTCTCTTTACACCTATTGCTGTCTTGGCATGGCTTCCGATTATATCTGCATTCCAGACTCGATTCCTGTTCAACGAGGCCTTCAATAGACACTTGCAGATCCAACCGATTCTTCAGGGGAAGAAGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1947

Amino Acids

225.66

Weight (kDa)

8.69

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 112 3.2e-08 Vta1 like
FKS1_dom1 PF14288 330 - 441 6e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 879 - 1072 1.5e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1079 - 1771 3.2e-215 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1211
AatII GACGTC 1 cut(s) 4516
Acc36I ACCTGC 1 cut(s) 3740
AccB1I GGYRCC 1 cut(s) 3328
AccB7I CCANNNNNTGG 5 cut(s) 1154, 1203, 1594, 3119, 3956
AccI GTMKAC 2 cut(s) 2069, 5340
AccII CGCG 3 cut(s) 369, 381, 2032
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 6 cut(s) 223, 656, 892, 1032, 2394, 4504
AclWI GGATC 6 cut(s) 2814, 3130, 3754, 4388, 5398, 5803
AcoI YGGCCR 3 cut(s) 2633, 3318, 4873
AcyI GRCGYC 1 cut(s) 4513
AfeI AGCGCT 1 cut(s) 4653
AflIII ACRYGT 3 cut(s) 379, 1750, 2739
AjnI CCWGG 4 cut(s) 534, 3357, 3462, 3940
AjuI GAANNNNNNNTTGG 2 cut(s) 5805, 5837
AleI CACNNNNGTG 1 cut(s) 2340
AloI GAACNNNNNNTCC 4 cut(s) 1842, 1874, 4670, 4702
Alw21I GWGCWC 1 cut(s) 3949
Alw26I GTCTC 8 cut(s) 859, 1064, 1302, 1711, 1975, 3253, 3759, 4184
Alw44I GTGCAC 1 cut(s) 3945
AlwI GGATC 6 cut(s) 2814, 3130, 3754, 4388, 5398, 5803
AlwNI CAGNNNCTG 2 cut(s) 535, 3083
Aor13HI TCCGGA 1 cut(s) 159
Aor51HI AGCGCT 1 cut(s) 4653
ApaLI GTGCAC 1 cut(s) 3945
AseI ATTAAT 3 cut(s) 1571, 3369, 5066
Asp700I GAANNNNTTC 6 cut(s) 705, 3458, 3636, 4089, 4156, 4778
AspA2I CCTAGG 1 cut(s) 2015
AspLEI GCGC 3 cut(s) 2032, 3471, 4654
AsuC2I CCSGG 1 cut(s) 3542
AsuII TTCGAA 1 cut(s) 266
AvaII GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
AvrII CCTAGG 1 cut(s) 2015
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 3949
BalI TGGCCA 2 cut(s) 2635, 3320
BanI GGYRCC 1 cut(s) 3328
BanII GRGCYC 2 cut(s) 27, 2248
BarI GAAGNNNNNNTAC 2 cut(s) 1810, 1842
BauI CACGAG 1 cut(s) 4002
BbsI GAAGAC 3 cut(s) 2595, 2864, 2964
Bbv12I GWGCWC 1 cut(s) 3949
BceAI ACGGC 3 cut(s) 4121, 4476, 4888
BcgI CGANNNNNNTGC 2 cut(s) 3062, 3096
BciT130I CCWGG 4 cut(s) 536, 3359, 3464, 3942
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 2 cut(s) 607, 3913
BcnI CCSGG 1 cut(s) 3542
BcoDI GTCTC 8 cut(s) 859, 1064, 1302, 1711, 1975, 3253, 3759, 4184
BfaI CTAG 9 cut(s) 1334, 1815, 2016, 3692, 4628, 4668, 4941, 5283, 5582
BfmI CTRYAG 6 cut(s) 305, 873, 1407, 1785, 3583, 4957
BfoI RGCGCY 1 cut(s) 4655
BfuAI ACCTGC 1 cut(s) 3740
BfuI GTATCC 1 cut(s) 563
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 3 cut(s) 703, 2905, 4437
BlnI CCTAGG 1 cut(s) 2015
BlpI GCTNAGC 1 cut(s) 3687
BmcAI AGTACT 3 cut(s) 752, 5650, 5676
Bme1390I CCNGG 5 cut(s) 536, 3359, 3464, 3542, 3942
Bme18I GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
BmiI GGNNCC 7 cut(s) 1944, 2247, 2528, 3330, 3545, 4781, 5636
BmrFI CCNGG 5 cut(s) 536, 3359, 3464, 3542, 3942
BmrI ACTGGG 3 cut(s) 1119, 4705, 4923
BmuI ACTGGG 3 cut(s) 1119, 4705, 4923
BoxI GACNNNNGTC 1 cut(s) 851
BpiI GAAGAC 3 cut(s) 2595, 2864, 2964
BpmI CTGGAG 4 cut(s) 3380, 3485, 3783, 4658
Bpu10I CCTNAGC 3 cut(s) 111, 4331, 4455
Bpu1102I GCTNAGC 1 cut(s) 3687
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 2 cut(s) 2897, 4790
BpuMI CCSGG 1 cut(s) 3542
BsaAI YACGTR 2 cut(s) 470, 4386
BsaBI GATNNNNATC 1 cut(s) 5402
BsaHI GRCGYC 1 cut(s) 4513
BsaI GGTCTC 4 cut(s) 1302, 1975, 3759, 4184
BsaWI WCCGGW 2 cut(s) 159, 951
BsaXI ACNNNNNCTCC 2 cut(s) 1345, 1375
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 9 cut(s) 923, 1114, 1731, 2636, 3034, 4711, 4918, 5168, 5656
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 1695, 1779, 3234
Bse8I GATNNNNATC 1 cut(s) 5402
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 4 cut(s) 536, 3359, 3464, 3942
BseJI GATNNNNATC 1 cut(s) 5402
BseMI GCAATG 3 cut(s) 1695, 1779, 3234
BseNI ACTGG 9 cut(s) 923, 1114, 1731, 2636, 3034, 4711, 4918, 5168, 5656
BseRI GAGGAG 3 cut(s) 2383, 3398, 3848
BseSI GKGCMC 1 cut(s) 3949
BseX3I CGGCCG 1 cut(s) 4873
BseYI CCCAGC 2 cut(s) 1854, 4349
Bsh1236I CGCG 3 cut(s) 369, 381, 2032
Bsh1285I CGRYCG 1 cut(s) 4876
BshNI GGYRCC 1 cut(s) 3328
BsiEI CGRYCG 1 cut(s) 4876
BsiHKAI GWGCWC 1 cut(s) 3949
BsiSI CCGG 4 cut(s) 160, 522, 952, 3541
BslFI GGGAC 4 cut(s) 733, 1255, 1928, 5159
BsmAI GTCTC 8 cut(s) 859, 1064, 1302, 1711, 1975, 3253, 3759, 4184
BsmBI CGTCTC 1 cut(s) 3253
BsmFI GGGAC 4 cut(s) 733, 1255, 1928, 5159
BsmI GAATGC 5 cut(s) 1390, 2396, 4058, 4749, 5759
Bso31I GGTCTC 4 cut(s) 1302, 1975, 3759, 4184
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 3 cut(s) 27, 2248, 3949
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3687
Bsp19I CCATGG 2 cut(s) 78, 3985
BspACI CCGC 6 cut(s) 223, 656, 892, 1032, 2394, 4504
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 3 cut(s) 369, 381, 2032
BspHI TCATGA 3 cut(s) 1641, 2160, 4390
BspLI GGNNCC 7 cut(s) 1944, 2247, 2528, 3330, 3545, 4781, 5636
BspMAI CTGCAG 2 cut(s) 309, 877
BspMI ACCTGC 1 cut(s) 3740
BspPI GGATC 6 cut(s) 2814, 3130, 3754, 4388, 5398, 5803
BspQI GCTCTTC 1 cut(s) 3510
BspT104I TTCGAA 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 3328
BspTNI GGTCTC 4 cut(s) 1302, 1975, 3759, 4184
BsrDI GCAATG 3 cut(s) 1695, 1779, 3234
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 9 cut(s) 923, 1114, 1731, 2636, 3034, 4711, 4918, 5168, 5656
BssAI RCCGGY 1 cut(s) 521
BssNAI GTATAC 1 cut(s) 2070
BssNI GRCGYC 1 cut(s) 4513
BssSI CACGAG 1 cut(s) 4002
BssT1I CCWWGG 7 cut(s) 78, 2015, 2338, 3985, 4507, 4689, 5440
Bst1107I GTATAC 1 cut(s) 2070
Bst2BI CACGAG 1 cut(s) 4002
Bst2UI CCWGG 4 cut(s) 536, 3359, 3464, 3942
Bst6I CTCTTC 2 cut(s) 1882, 3510
BstACI GRCGYC 1 cut(s) 4513
BstAPI GCANNNNNTGC 1 cut(s) 3683
BstBAI YACGTR 2 cut(s) 470, 4386
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 8 cut(s) 138, 197, 584, 1149, 1585, 2051, 3401, 4824
BstDSI CCRYGG 2 cut(s) 78, 3985
BstEII GGTNACC 1 cut(s) 954
BstENI CCTNNNNNAGG 1 cut(s) 4334
BstFNI CGCG 3 cut(s) 369, 381, 2032
BstH2I RGCGCY 1 cut(s) 4655
BstHHI GCGC 3 cut(s) 2032, 3471, 4654
BstMAI GTCTC 8 cut(s) 859, 1064, 1302, 1711, 1975, 3253, 3759, 4184
BstMCI CGRYCG 1 cut(s) 4876
BstNI CCWGG 4 cut(s) 536, 3359, 3464, 3942
BstNSI RCATGY 9 cut(s) 586, 1105, 1587, 1754, 2743, 3308, 3430, 3776, 4402
BstPAI GACNNNNGTC 1 cut(s) 851
BstPI GGTNACC 1 cut(s) 954
BstSCI CCNGG 5 cut(s) 534, 3357, 3462, 3540, 3940
BstSFI CTRYAG 6 cut(s) 305, 873, 1407, 1785, 3583, 4957
BstSLI GKGCMC 1 cut(s) 3949
BstUI CGCG 3 cut(s) 369, 381, 2032
BstV2I GAAGAC 3 cut(s) 2595, 2864, 2964
BstX2I RGATCY 6 cut(s) 703, 2806, 2905, 3122, 4437, 5808
BstXI CCANNNNNNTGG 5 cut(s) 1474, 3450, 3743, 3986, 5601
BstYI RGATCY 6 cut(s) 703, 2806, 2905, 3122, 4437, 5808
BstZ17I GTATAC 1 cut(s) 2070
BstZI CGGCCG 1 cut(s) 4873
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 563
BtgI CCRYGG 2 cut(s) 78, 3985
BtsI GCAGTG 3 cut(s) 91, 882, 5439
BveI ACCTGC 1 cut(s) 3740
Cac8I GCNNGC 8 cut(s) 138, 197, 584, 1149, 1585, 2051, 3401, 4824
CaiI CAGNNNCTG 2 cut(s) 535, 3083
CciI TCATGA 3 cut(s) 1641, 2160, 4390
CfoI GCGC 3 cut(s) 2032, 3471, 4654
Cfr10I RCCGGY 1 cut(s) 521
CseI GACGC 3 cut(s) 387, 1319, 3270
CspCI CAANNNNNGTGG 4 cut(s) 1593, 1628, 2406, 2441
DraI TTTAAA 1 cut(s) 904
EaeI YGGCCR 3 cut(s) 2633, 3318, 4873
EagI CGGCCG 1 cut(s) 4873
Eam1104I CTCTTC 2 cut(s) 1882, 3510
EarI CTCTTC 2 cut(s) 1882, 3510
EclXI CGGCCG 1 cut(s) 4873
Eco130I CCWWGG 7 cut(s) 78, 2015, 2338, 3985, 4507, 4689, 5440
Eco147I AGGCCT 1 cut(s) 5789
Eco24I GRGCYC 2 cut(s) 27, 2248
Eco31I GGTCTC 4 cut(s) 1302, 1975, 3759, 4184
Eco32I GATATC 3 cut(s) 574, 1639, 4584
Eco47I GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
Eco47III AGCGCT 1 cut(s) 4653
Eco52I CGGCCG 1 cut(s) 4873
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 954
EcoNI CCTNNNNNAGG 1 cut(s) 4334
EcoO109I RGGNCCY 4 cut(s) 532, 3781, 5037, 5634
EcoO65I GGTNACC 1 cut(s) 954
EcoRI GAATTC 2 cut(s) 3139, 5663
EcoRII CCWGG 4 cut(s) 534, 3357, 3462, 3940
EcoRV GATATC 3 cut(s) 574, 1639, 4584
EcoT14I CCWWGG 7 cut(s) 78, 2015, 2338, 3985, 4507, 4689, 5440
EcoT22I ATGCAT 3 cut(s) 3297, 3679, 3700
EcoT38I GRGCYC 2 cut(s) 27, 2248
ErhI CCWWGG 7 cut(s) 78, 2015, 2338, 3985, 4507, 4689, 5440
Esp3I CGTCTC 1 cut(s) 3253
FaqI GGGAC 4 cut(s) 733, 1255, 1928, 5159
FauNDI CATATG 8 cut(s) 1080, 1087, 1153, 1846, 3297, 3721, 5059, 5689
FbaI TGATCA 2 cut(s) 607, 3913
FblI GTMKAC 2 cut(s) 2069, 5340
FriOI GRGCYC 2 cut(s) 27, 2248
FspBI CTAG 9 cut(s) 1334, 1815, 2016, 3692, 4628, 4668, 4941, 5283, 5582
GlaI GCGC 3 cut(s) 2031, 3470, 4653
GsaI CCCAGC 2 cut(s) 1858, 4353
GsuI CTGGAG 4 cut(s) 3380, 3485, 3783, 4658
HaeII RGCGCY 1 cut(s) 4655
HapII CCGG 4 cut(s) 160, 522, 952, 3541
HgaI GACGC 3 cut(s) 387, 1319, 3270
HhaI GCGC 3 cut(s) 2032, 3471, 4654
Hin1I GRCGYC 1 cut(s) 4513
Hin6I GCGC 3 cut(s) 2030, 3469, 4652
HinP1I GCGC 3 cut(s) 2030, 3469, 4652
HincII GTYRAC 1 cut(s) 1107
HindII GTYRAC 1 cut(s) 1107
HindIII AAGCTT 2 cut(s) 1525, 3935
HpaI GTTAAC 1 cut(s) 1107
HpaII CCGG 4 cut(s) 160, 522, 952, 3541
Hpy99I CGWCG 1 cut(s) 4374
HpyCH4IV ACGT 7 cut(s) 469, 1501, 3828, 4369, 4385, 4513, 4841
HpySE526I ACGT 7 cut(s) 469, 1501, 3828, 4369, 4385, 4513, 4841
Hsp92I GRCGYC 1 cut(s) 4513
HspAI GCGC 3 cut(s) 2030, 3469, 4652
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 2 cut(s) 607, 3913
KspAI GTTAAC 1 cut(s) 1107
LguI GCTCTTC 1 cut(s) 3510
LmnI GCTCC 9 cut(s) 162, 422, 1542, 2251, 2575, 3385, 3466, 5010, 5116
MaeI CTAG 9 cut(s) 1334, 1815, 2016, 3692, 4628, 4668, 4941, 5283, 5582
MaeII ACGT 7 cut(s) 469, 1501, 3828, 4369, 4385, 4513, 4841
MfeI CAATTG 1 cut(s) 4814
MflI RGATCY 6 cut(s) 703, 2806, 2905, 3122, 4437, 5808
MhlI GDGCHC 3 cut(s) 27, 2248, 3949
MlsI TGGCCA 2 cut(s) 2635, 3320
MluI ACGCGT 1 cut(s) 379
MluNI TGGCCA 2 cut(s) 2635, 3320
MlyI GAGTC 4 cut(s) 1178, 1352, 4665, 5761
MmeI TCCRAC 4 cut(s) 1388, 3263, 3477, 5836
Mox20I TGGCCA 2 cut(s) 2635, 3320
Mph1103I ATGCAT 3 cut(s) 3297, 3679, 3700
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 6 cut(s) 705, 3458, 3636, 4089, 4156, 4778
MscI TGGCCA 2 cut(s) 2635, 3320
MslI CAYNNNNRTG 5 cut(s) 267, 2340, 4028, 4263, 4893
Msp20I TGGCCA 2 cut(s) 2635, 3320
MspA1I CMGCKG 3 cut(s) 1247, 2053, 4822
MspI CCGG 4 cut(s) 160, 522, 952, 3541
MspR9I CCNGG 5 cut(s) 536, 3359, 3464, 3542, 3942
MunI CAATTG 1 cut(s) 4814
Mva1269I GAATGC 5 cut(s) 1390, 2396, 4058, 4749, 5759
MvaI CCWGG 4 cut(s) 536, 3359, 3464, 3942
MvnI CGCG 3 cut(s) 369, 381, 2032
NciI CCSGG 1 cut(s) 3542
NcoI CCATGG 2 cut(s) 78, 3985
NdeI CATATG 8 cut(s) 1080, 1087, 1153, 1846, 3297, 3721, 5059, 5689
NlaIV GGNNCC 7 cut(s) 1944, 2247, 2528, 3330, 3545, 4781, 5636
NmeAIII GCCGAG 1 cut(s) 1249
NmuCI GTSAC 3 cut(s) 443, 4201, 4493
NsiI ATGCAT 3 cut(s) 3297, 3679, 3700
NspI RCATGY 9 cut(s) 586, 1105, 1587, 1754, 2743, 3308, 3430, 3776, 4402
NspV TTCGAA 1 cut(s) 266
OliI CACNNNNGTG 1 cut(s) 2340
PaeI GCATGC 2 cut(s) 586, 1587
PagI TCATGA 3 cut(s) 1641, 2160, 4390
PceI AGGCCT 1 cut(s) 5789
PciI ACATGT 2 cut(s) 1750, 2739
PciSI GCTCTTC 1 cut(s) 3510
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PctI GAATGC 5 cut(s) 1390, 2396, 4058, 4749, 5759
PdmI GAANNNNTTC 6 cut(s) 705, 3458, 3636, 4089, 4156, 4778
PflMI CCANNNNNTGG 5 cut(s) 1154, 1203, 1594, 3119, 3956
PfoI TCCNGGA 1 cut(s) 3462
PleI GAGTC 4 cut(s) 1177, 1352, 4665, 5761
PpsI GAGTC 4 cut(s) 1177, 1352, 4665, 5761
Ppu21I YACGTR 2 cut(s) 470, 4386
PpuMI RGGWCCY 2 cut(s) 3781, 5037
PscI ACATGT 2 cut(s) 1750, 2739
PshAI GACNNNNGTC 1 cut(s) 851
PshBI ATTAAT 3 cut(s) 1571, 3369, 5066
PsiI TTATAA 1 cut(s) 1211
Psp5II RGGWCCY 2 cut(s) 3781, 5037
Psp6I CCWGG 4 cut(s) 534, 3357, 3462, 3940
PspEI GGTNACC 1 cut(s) 954
PspFI CCCAGC 2 cut(s) 1854, 4349
PspGI CCWGG 4 cut(s) 534, 3357, 3462, 3940
PspN4I GGNNCC 7 cut(s) 1944, 2247, 2528, 3330, 3545, 4781, 5636
PspPPI RGGWCCY 2 cut(s) 3781, 5037
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 2 cut(s) 309, 877
PstNI CAGNNNCTG 2 cut(s) 535, 3083
PsuI RGATCY 6 cut(s) 703, 2806, 2905, 3122, 4437, 5808
PvuII CAGCTG 3 cut(s) 1247, 2053, 4822
RseI CAYNNNNRTG 5 cut(s) 267, 2340, 4028, 4263, 4893
SapI GCTCTTC 1 cut(s) 3510
ScaI AGTACT 3 cut(s) 752, 5650, 5676
SchI GAGTC 4 cut(s) 1178, 1352, 4665, 5761
ScrFI CCNGG 5 cut(s) 536, 3359, 3464, 3542, 3942
SduI GDGCHC 3 cut(s) 27, 2248, 3949
SfcI CTRYAG 6 cut(s) 305, 873, 1407, 1785, 3583, 4957
SfuI TTCGAA 1 cut(s) 266
SinI GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
SmiMI CAYNNNNRTG 5 cut(s) 267, 2340, 4028, 4263, 4893
SmlI CTYRAG 2 cut(s) 2876, 4769
SmoI CTYRAG 2 cut(s) 2876, 4769
SphI GCATGC 2 cut(s) 586, 1587
SseBI AGGCCT 1 cut(s) 5789
SsiI CCGC 6 cut(s) 223, 656, 892, 1032, 2394, 4504
SspI AATATT 3 cut(s) 595, 820, 2404
SspMI CTAG 9 cut(s) 1334, 1815, 2016, 3692, 4628, 4668, 4941, 5283, 5582
StuI AGGCCT 1 cut(s) 5789
StyD4I CCNGG 5 cut(s) 534, 3357, 3462, 3540, 3940
StyI CCWWGG 7 cut(s) 78, 2015, 2338, 3985, 4507, 4689, 5440
TaiI ACGT 7 cut(s) 472, 1504, 3831, 4372, 4388, 4516, 4844
TaqII GACCGA 1 cut(s) 3086
TauI GCSGC 3 cut(s) 225, 659, 1034
TseFI GTSAC 3 cut(s) 443, 4201, 4493
Tsp45I GTSAC 3 cut(s) 443, 4201, 4493
TspGWI ACGGA 4 cut(s) 361, 844, 3806, 4629
Van91I CCANNNNNTGG 5 cut(s) 1154, 1203, 1594, 3119, 3956
VneI GTGCAC 1 cut(s) 3945
VpaK11BI GGWCC 6 cut(s) 256, 524, 1942, 3781, 5037, 5254
VspI ATTAAT 3 cut(s) 1571, 3369, 5066
XagI CCTNNNNNAGG 1 cut(s) 4334
XbaI TCTAGA 2 cut(s) 1333, 1814
XceI RCATGY 9 cut(s) 586, 1105, 1587, 1754, 2743, 3308, 3430, 3776, 4402
XcmI CCANNNNNNNNNTGG 1 cut(s) 2515
XmaJI CCTAGG 1 cut(s) 2015
XmiI GTMKAC 2 cut(s) 2069, 5340
XmnI GAANNNNTTC 6 cut(s) 705, 3458, 3636, 4089, 4156, 4778
XspI CTAG 9 cut(s) 1334, 1815, 2016, 3692, 4628, 4668, 4941, 5283, 5582
ZraI GACGTC 1 cut(s) 4514
ZrmI AGTACT 3 cut(s) 752, 5650, 5676
Zsp2I ATGCAT 3 cut(s) 3297, 3679, 3700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.