FvH4_3g16960

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016003
Physical Location & Seq
Forward (+)
10690255 .. 10706551
16297 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16960.t1

Sequence Viewer

Length: 5856 bp
ATGTCTGAGATTGTGGTTGCAGAACCCATCTATGATGGTGGAGCAGAGGAAAAAAGACCCATTACAAGGTATCTCACTATGGGAAGTGGGAGTGATCACAATCATGTTCCTGAGCCATTTGAGAGCGAGAGATTACCTGCCACTTTGGCCTCAAAAATCCAGAGCTTTCTTCGGGTTGCCAACTTGATCGAAAGCGAGGAGCCTCGGATTGCTTATCTATGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCATTCGAATGGGCGGGGTGTTAGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACATTTAGGAAACGGAAGGAAAAGAGTGATATACGTGAGTTGAGACGTGTCTATCATGCTTACAAGGAGTACATCATAAAACATGAAGGAGCATTTAACACAGAAAATAGTCACAGAGAGAAGTTGATAAATGCACGTATAATTGGTTCTGTGTTGTTCGAAGTCTTGAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTTGCTAATAGAGGTGGTATCCAGACAAAACCTAATGATCTCTTTGGCATATATAACATTCTACCACTTGATCAAGGAGGTGCTCAGCAAGCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAATACGACACATTCGTGGCATACCTTCCAACGAAGATTTTCAGAAACATGGCAATTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTTTACTCCTTGCTAATATTCACAGACGGAAAACTCAGAAGCAGACATCTGTCTCAAAATTAGGTGATGCTGCAGTGGATGAGTTAATGAGGAGATTTTTCAAAAATTATACAAATTGGTGCAAATTTTTGGGAAGGAAAAGTAACATCCGGTTGCCTTATGTGAAACAAGAAGCCCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGGGAGGCTGCAAACTTGCGATTCATGCCAGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAGGTCATGCCGGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTCGTTACCCCCATATATGGGGTTATAAGAGAGGAAGCCAAGAAAAGCAAAGGTGGGACAGCTGACCATTCCACGTGGAGAAATTATGATGATCTGAATGAGTATTTTTGGTCTCCTGATTGTTTCGAAATCGGTTGGCCCATGCATCTAGACCATGATTTTTTTTGTATACATTCTCCAAAAAAGTCCAATGCTAAGAAAGCTTCAGCATCCACTGCTCCTGTAGAAGAAAGAAGGAAGGAAGACGGGGAGGAAGATGAAGTTGGGGTTACTAAAGAAGAAGTCCGTGAACCCAAATGGTTGGGAAAAACGAATTTTGTAGAAGTACGTTCTTTTTGGCAGATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCCCTTCAGGCATTGATAATTATGGCATGCCATGAAGTGGAATCTCCACTTCAATTATTTGACAAAGTTATATTTGAGGATATCATGAGTATCTTCATTACGTCTGCATTCCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACGCTGGACTTCTCTGCAAAAGTAAAGCATGTTATGAAGCTGGGTGTTGCAATGATATGGACTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATTCCACAACATATGGAAGCTGGCTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATCTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTGTTCGCAAATACATTGAGATCTCAAATTACCGTATATGCACCATTCTCTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGAATGCAAGAAAGCCAGCTTTCAGTGTTGAAGTATACATTGTTTTGGGTGCTTATACTGCTGAGCAAATTCTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAACCAACAAAGCAGATCATGAAAATTGGTGTACAAATGTATGATTGGCATGAGCTTTTTCCAAAAGTTAAGAATAATGCTGGTGCAATTGCTGCTATATGGGCTCCAATAATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATTTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGGACGTTAGGAATGTTGAGGAGTAGATTTCACACCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATCAAGAAATGACGGAAGGAGAAAAATAGGTTTCTTTTACAATACATTCAGAAAGGTCTCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCATCAGAATTGTTTTCCGGCATAGTTCGTTGGCCTGTTTTTCTTCTTGCAAATAAGTTTTCAACAGCCCTCAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTATCAGAAAACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTATGAATCGTTGAAGTATGTCCTTGAAATCCTTATTATTGGGGATCTTGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAAAAAGCATCGCAAAATCAAGTCTTCTTGAGGACTTTCGGATGATTAAAGTTCCGGATCTTCTTGCTAAATGTATTGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCATGGTAAAGTTGCGAAAGTTCTCCAAGACATATTTGAGCTTGTAACCAATGATATGATGACTAGTGGCTTTAGGATATTAGAGTTGCTGGATTCATCCCAACAGACCGAAACAGATTCTGCTTATTTTTCTGGAAATATTGAATCCCCATTATTTGGATCTGCTGGTGGCAGGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTAAATGAGCAGATCAAGCGCTTTCTTCTGTTGCTTACTGTCCAAGATACAGCAATGGACATTCCAAGTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAATATGCCAGGTGCACCCAAAGTGGCCAATATGGTGCCATTCAGTGTTATGACACCACACTACCTGGAGGATATTAATTTTTCAAAGGAGGAGCTTCATTCAAGCCAACGGGAGGTTTCTATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCCTGGAGCGCATGGGTTATGAAAATTTGGATGAACTGGAGAGGGACAAACAGGAAGAGCTTAGAAATTGGGCTTCTTTCCGGGGCCAAACACTAAGCAGAACAGTCAGAGGAATGATGTATTATAGAGAAGCCCTAAAACTGCAAGCATTTCTAGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATCCACTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTTGTTACTTGTCAATTGTTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAATAGATACCCATCTCTCCGTGTCGCTTATGTCGAGGAGAAAGAAGAGATAGTGGACAACAAGCCTCACAAAGTTTATTCTTCTGTCTTGGTTAAAGCTATCCCTGATTTTGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTCCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAAAACCATGGAATAATCTTCACTCGTGGCGAAGCTCTTCAAACCATTGATATGAACCAAGACAGTTATTTGGAAGAGGCATTCAAGATGAGAAATCTTTTGCAAGAATTTCTTCAGAACCAAGGACGCCGACCTCCTATACTACTTGGTTTGAGGGAACACATATTTACAGGAAGTGTATCTTCTCTTGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTAAGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGAATAAGCAAAGCATCAAAAACTATTAACCTGAGTGAGGATGTGTTTGCGGGATACAACTCTACCTTACGACGAGGATGGATCACGTATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAATTTGAAGCCAAGGTTGCTAATGGAAACAGTGAACAAACTATAAGCCGTGATATATTCCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTATGGACAGCTATACCTTGTCCTCAGTGGCTTGGAGAAAGCGCTTGTAATTGAAGCTAGACTGCAGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGCTATTAACAGGAATGCCAATGGTGATGGAAATTGGACTTGAGAAAGGATTCCTTAATGCACTAAAAGATTTTGTCCTCATGCAACTGCAGCTGGCTTCTGTTTTTTTCACATTCTCCTTTGGAACAAAAATCCATTATTACGGCCGAACAATTATGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTCGTTGTGTTTCACACTAGCTTCACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTTGTCAAAGGATTTGAGTTATTGCTCTTGTTGATTGTTTATGACTTGTTTAGGCGGTCCTATGAGAGCAGTATGGCGTATGTATTAATCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTTGCTCCATTTCTTTTTAATCCATCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGACTGGAATAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCAACAAGAAAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGACTTCTAGGTTGTTTGAAATACTCCTATCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCACGAATTTTCTGGTTTATTTGCTTTCATGGATTGTGATCCTCGTTGTATTCTTGTTAGTAAAGGCAGTGAACCTCGGAAGGCAACAGTTCAGTGCTAGATATCACCTCGTGTTTAGGCTATTCAAAGCAACCCTCTTCTTGGGTGTTCTTTCCATCATCATATCTCTCTCTCTTGTGTGTCATCTATCTTGGAGGGACTTAGTTGTCTGCTGCCTAGCATTTTTGCCAACAGGATGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAATACTGGATTGTGGGAATTCACCCGAGTACTTGCTAAAACATATGACTACGGAATGGGGGTTGTTCTGTTTGCACCTATTGCCGTCTTGGCATGGCTTCCGATTATATCAGCATTCCAGACTCGTTTCCTGTTCAACGAGGCCTTCAATAGACACTTGCAGATCCAACCAATTCTTCAGGGGAAGAAGAAGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1952

Amino Acids

225.59

Weight (kDa)

8.54

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 122 1e-08 Vta1 like
FKS1_dom1 PF14288 329 - 440 3e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 882 - 1075 4.6e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1082 - 1171 8.2e-25 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1172 - 1847 2.6e-194 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1208
AasI GACNNNNNNGTC 1 cut(s) 5579
AatII GACGTC 1 cut(s) 4525
Acc36I ACCTGC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 3337
AccB7I CCANNNNNTGG 3 cut(s) 1603, 3128, 3965
AccI GTMKAC 3 cut(s) 1351, 2078, 5349
AccII CGCG 1 cut(s) 2041
AccIII TCCGGA 1 cut(s) 2911
AciI CCGC 7 cut(s) 223, 274, 653, 2403, 4358, 4513, 5044
AclWI GGATC 8 cut(s) 2823, 2922, 3139, 3763, 4397, 5201, 5407, 5812
AcoI YGGCCR 2 cut(s) 3327, 4882
AcuI CTGAAG 7 cut(s) 728, 1371, 1556, 3654, 4085, 4522, 5816
AcvI CACGTG 1 cut(s) 1257
AcyI GRCGYC 2 cut(s) 4114, 4522
AdeI CACNNNGTG 1 cut(s) 5485
AfaI GTAC 8 cut(s) 404, 749, 1509, 2184, 2273, 2747, 2753, 5685
AfeI AGCGCT 2 cut(s) 3203, 4662
AflIII ACRYGT 2 cut(s) 379, 2748
AhlI ACTAGT 1 cut(s) 3035
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 4 cut(s) 3310, 3366, 3471, 3949
AjuI GAANNNNNNNTTGG 4 cut(s) 1428, 1460, 5814, 5846
AleI CACNNNNGTG 2 cut(s) 678, 2349
AloI GAACNNNNNNTCC 2 cut(s) 4679, 4711
Alw21I GWGCWC 2 cut(s) 619, 3319
Alw26I GTCTC 9 cut(s) 370, 856, 1061, 1299, 1720, 2494, 3262, 3768, 4193
Alw44I GTGCAC 1 cut(s) 3315
AlwI GGATC 8 cut(s) 2823, 2922, 3139, 3763, 4397, 5201, 5407, 5812
AlwNI CAGNNNCTG 1 cut(s) 3092
Ama87I CYCGRG 1 cut(s) 5679
Aor13HI TCCGGA 1 cut(s) 2911
Aor51HI AGCGCT 2 cut(s) 3203, 4662
AoxI GGCC 9 cut(s) 147, 531, 1319, 2642, 3327, 3553, 4882, 5642, 5796
ApaLI GTGCAC 1 cut(s) 3315
AseI ATTAAT 2 cut(s) 3378, 5075
Asp700I GAANNNNTTC 6 cut(s) 702, 3467, 3645, 4023, 4098, 4787
AspA2I CCTAGG 1 cut(s) 2024
AspLEI GCGC 4 cut(s) 2041, 3204, 3480, 4663
AsuC2I CCSGG 1 cut(s) 3551
AsuHPI GGTGA 9 cut(s) 875, 1064, 2336, 2363, 3932, 4204, 4774, 5471, 5668
AsuII TTCGAA 3 cut(s) 266, 492, 1308
AvaI CYCGRG 1 cut(s) 5679
AvaII GGWCC 7 cut(s) 256, 524, 1951, 3790, 3953, 5046, 5263
AvrII CCTAGG 1 cut(s) 2024
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 3319
BalI TGGCCA 1 cut(s) 3329
BanI GGYRCC 1 cut(s) 3337
BanII GRGCYC 1 cut(s) 2257
BarI GAAGNNNNNNTAC 2 cut(s) 1819, 1851
BauI CACGAG 2 cut(s) 4011, 5483
BbrPI CACGTG 1 cut(s) 1257
BbsI GAAGAC 4 cut(s) 509, 1431, 2873, 2973
Bbv12I GWGCWC 2 cut(s) 619, 3319
BceAI ACGGC 3 cut(s) 4485, 4897, 5723
BciT130I CCWGG 4 cut(s) 3312, 3368, 3473, 3951
BciVI GTATCC 2 cut(s) 563, 4355
BclI TGATCA 3 cut(s) 94, 604, 3922
BcnI CCSGG 1 cut(s) 3551
BcoDI GTCTC 9 cut(s) 370, 856, 1061, 1299, 1720, 2494, 3262, 3768, 4193
BcuI ACTAGT 1 cut(s) 3035
BfmI CTRYAG 7 cut(s) 305, 870, 1404, 1794, 4682, 4826, 4966
BfoI RGCGCY 2 cut(s) 3205, 4664
BfuAI ACCTGC 1 cut(s) 145
BfuI GTATCC 2 cut(s) 563, 4355
BglI GCCNNNNNGGC 2 cut(s) 146, 5744
BglII AGATCT 2 cut(s) 1977, 4446
BlnI CCTAGG 1 cut(s) 2024
BlpI GCTNAGC 3 cut(s) 618, 2105, 3696
BmcAI AGTACT 2 cut(s) 749, 5685
Bme1390I CCNGG 5 cut(s) 3312, 3368, 3473, 3551, 3951
Bme18I GGWCC 7 cut(s) 256, 524, 1951, 3790, 3953, 5046, 5263
BmeT110I CYCGRG 1 cut(s) 5679
BmgBI CACGTC 1 cut(s) 380
BmiI GGNNCC 7 cut(s) 201, 1953, 2256, 2537, 3339, 3554, 5645
BmrFI CCNGG 5 cut(s) 3312, 3368, 3473, 3551, 3951
BmrI ACTGGG 2 cut(s) 4714, 4932
BmuI ACTGGG 2 cut(s) 4714, 4932
BoxI GACNNNNGTC 1 cut(s) 848
BpiI GAAGAC 4 cut(s) 509, 1431, 2873, 2973
BpmI CTGGAG 4 cut(s) 3389, 3494, 3527, 3792
Bpu10I CCTNAGC 1 cut(s) 111
Bpu1102I GCTNAGC 3 cut(s) 618, 2105, 3696
Bpu14I TTCGAA 3 cut(s) 266, 492, 1308
BpuEI CTTGAG 2 cut(s) 2906, 4799
BpuMI CCSGG 1 cut(s) 3551
BsaAI YACGTR 4 cut(s) 368, 470, 1257, 4395
BsaBI GATNNNNATC 3 cut(s) 99, 3817, 5411
BsaHI GRCGYC 2 cut(s) 4114, 4522
BsaI GGTCTC 4 cut(s) 1299, 2494, 3768, 4193
BsaWI WCCGGW 2 cut(s) 948, 2911
Bse118I RCCGGY 2 cut(s) 521, 1144
Bse1I ACTGG 7 cut(s) 1111, 3510, 4720, 4927, 5177, 5186, 5665
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 1704, 1788, 3243
Bse8I GATNNNNATC 3 cut(s) 99, 3817, 5411
BseAI TCCGGA 1 cut(s) 2911
BseBI CCWGG 4 cut(s) 3312, 3368, 3473, 3951
BseJI GATNNNNATC 3 cut(s) 99, 3817, 5411
BseMI GCAATG 3 cut(s) 1704, 1788, 3243
BseNI ACTGG 7 cut(s) 1111, 3510, 4720, 4927, 5177, 5186, 5665
BseRI GAGGAG 5 cut(s) 212, 904, 2392, 3407, 3857
BseSI GKGCMC 1 cut(s) 3319
BseX3I CGGCCG 1 cut(s) 4882
BseYI CCCAGC 1 cut(s) 1771
Bsh1236I CGCG 1 cut(s) 2041
Bsh1285I CGRYCG 1 cut(s) 4885
BshFI GGCC 9 cut(s) 149, 533, 1321, 2644, 3329, 3555, 4884, 5644, 5798
BshNI GGYRCC 1 cut(s) 3337
BsiEI CGRYCG 1 cut(s) 4885
BsiHKAI GWGCWC 2 cut(s) 619, 3319
BsiHKCI CYCGRG 1 cut(s) 5679
BsiSI CCGG 6 cut(s) 522, 949, 1145, 2628, 2912, 3550
BslFI GGGAC 5 cut(s) 1252, 1937, 3527, 5168, 5585
BsmAI GTCTC 9 cut(s) 370, 856, 1061, 1299, 1720, 2494, 3262, 3768, 4193
BsmBI CGTCTC 2 cut(s) 370, 3262
BsmFI GGGAC 5 cut(s) 1252, 1937, 3527, 5168, 5585
BsmI GAATGC 6 cut(s) 1673, 2052, 2405, 4067, 4758, 5768
BsnI GGCC 9 cut(s) 149, 533, 1321, 2644, 3329, 3555, 4884, 5644, 5798
Bso31I GGTCTC 4 cut(s) 1299, 2494, 3768, 4193
BsoBI CYCGRG 1 cut(s) 5679
Bsp119I TTCGAA 3 cut(s) 266, 492, 1308
Bsp1286I GDGCHC 3 cut(s) 619, 2257, 3319
Bsp13I TCCGGA 1 cut(s) 2911
Bsp1407I TGTACA 1 cut(s) 2182
Bsp1720I GCTNAGC 3 cut(s) 618, 2105, 3696
Bsp19I CCATGG 1 cut(s) 3994
BspACI CCGC 7 cut(s) 223, 274, 653, 2403, 4358, 4513, 5044
BspANI GGCC 9 cut(s) 149, 533, 1321, 2644, 3329, 3555, 4884, 5644, 5798
BspEI TCCGGA 1 cut(s) 2911
BspFNI CGCG 1 cut(s) 2041
BspHI TCATGA 3 cut(s) 1650, 2169, 4399
BspLI GGNNCC 7 cut(s) 201, 1953, 2256, 2537, 3339, 3554, 5645
BspMAI CTGCAG 4 cut(s) 309, 874, 4686, 4830
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 8 cut(s) 2823, 2922, 3139, 3763, 4397, 5201, 5407, 5812
BspQI GCTCTTC 2 cut(s) 3519, 4029
BspT104I TTCGAA 3 cut(s) 266, 492, 1308
BspT107I GGYRCC 1 cut(s) 3337
BspTNI GGTCTC 4 cut(s) 1299, 2494, 3768, 4193
BsrDI GCAATG 3 cut(s) 1704, 1788, 3243
BsrFI RCCGGY 2 cut(s) 521, 1144
BsrGI TGTACA 1 cut(s) 2182
BsrI ACTGG 7 cut(s) 1111, 3510, 4720, 4927, 5177, 5186, 5665
BssAI RCCGGY 2 cut(s) 521, 1144
BssNAI GTATAC 2 cut(s) 1352, 2079
BssNI GRCGYC 2 cut(s) 4114, 4522
BssSI CACGAG 2 cut(s) 4011, 5483
BssT1I CCWWGG 7 cut(s) 2024, 2347, 3994, 4108, 4464, 4516, 4698
Bst1107I GTATAC 2 cut(s) 1352, 2079
Bst2BI CACGAG 2 cut(s) 4011, 5483
Bst2UI CCWGG 4 cut(s) 3312, 3368, 3473, 3951
Bst6I CTCTTC 6 cut(s) 1891, 3519, 3846, 4029, 4056, 5516
BstACI GRCGYC 2 cut(s) 4114, 4522
BstAPI GCANNNNNTGC 3 cut(s) 1397, 2243, 5735
BstAUI TGTACA 1 cut(s) 2182
BstBAI YACGTR 4 cut(s) 368, 470, 1257, 4395
BstBI TTCGAA 3 cut(s) 266, 492, 1308
BstC8I GCNNGC 7 cut(s) 624, 1146, 1594, 1865, 2060, 3615, 4833
BstDSI CCRYGG 1 cut(s) 3994
BstENI CCTNNNNNAGG 1 cut(s) 4343
BstFNI CGCG 1 cut(s) 2041
BstH2I RGCGCY 2 cut(s) 3205, 4664
BstHHI GCGC 4 cut(s) 2041, 3204, 3480, 4663
BstMAI GTCTC 9 cut(s) 370, 856, 1061, 1299, 1720, 2494, 3262, 3768, 4193
BstMCI CGRYCG 1 cut(s) 4885
BstNI CCWGG 4 cut(s) 3312, 3368, 3473, 3951
BstNSI RCATGY 7 cut(s) 1102, 1596, 1763, 2752, 3439, 3785, 4411
BstPAI GACNNNNGTC 1 cut(s) 848
BstSCI CCNGG 5 cut(s) 3310, 3366, 3471, 3549, 3949
BstSFI CTRYAG 7 cut(s) 305, 870, 1404, 1794, 4682, 4826, 4966
BstSLI GKGCMC 1 cut(s) 3319
BstUI CGCG 1 cut(s) 2041
BstV2I GAAGAC 4 cut(s) 509, 1431, 2873, 2973
BstX2I RGATCY 6 cut(s) 1977, 2815, 2914, 3131, 4446, 5817
BstXI CCANNNNNNTGG 4 cut(s) 1483, 3459, 3995, 5610
BstYI RGATCY 6 cut(s) 1977, 2815, 2914, 3131, 4446, 5817
BstZ17I GTATAC 2 cut(s) 1352, 2079
BstZI CGGCCG 1 cut(s) 4882
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 2 cut(s) 563, 4355
BsuRI GGCC 9 cut(s) 149, 533, 1321, 2644, 3329, 3555, 4884, 5644, 5798
BtgI CCRYGG 1 cut(s) 3994
BtgZI GCGATG 1 cut(s) 2851
BtrI CACGTC 1 cut(s) 380
BtsI GCAGTG 3 cut(s) 879, 1395, 5448
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 7 cut(s) 624, 1146, 1594, 1865, 2060, 3615, 4833
CaiI CAGNNNCTG 1 cut(s) 3092
CciI TCATGA 3 cut(s) 1650, 2169, 4399
CfoI GCGC 4 cut(s) 2041, 3204, 3480, 4663
Cfr10I RCCGGY 2 cut(s) 521, 1144
CseI GACGC 2 cut(s) 3279, 4122
Csp6I GTAC 8 cut(s) 403, 748, 1508, 2183, 2272, 2746, 2752, 5684
CviQI GTAC 8 cut(s) 403, 748, 1508, 2183, 2272, 2746, 2752, 5684
DraIII CACNNNGTG 1 cut(s) 5485
DrdI GACNNNNNNGTC 1 cut(s) 5579
DseDI GACNNNNNNGTC 1 cut(s) 5579
EaeI YGGCCR 2 cut(s) 3327, 4882
EagI CGGCCG 1 cut(s) 4882
Eam1104I CTCTTC 6 cut(s) 1891, 3519, 3846, 4029, 4056, 5516
EarI CTCTTC 6 cut(s) 1891, 3519, 3846, 4029, 4056, 5516
EciI GGCGGA 1 cut(s) 4502
EclXI CGGCCG 1 cut(s) 4882
Eco130I CCWWGG 7 cut(s) 2024, 2347, 3994, 4108, 4464, 4516, 4698
Eco147I AGGCCT 1 cut(s) 5798
Eco24I GRGCYC 1 cut(s) 2257
Eco31I GGTCTC 4 cut(s) 1299, 2494, 3768, 4193
Eco32I GATATC 3 cut(s) 1648, 4593, 5477
Eco47I GGWCC 7 cut(s) 256, 524, 1951, 3790, 3953, 5046, 5263
Eco47III AGCGCT 2 cut(s) 3203, 4662
Eco52I CGGCCG 1 cut(s) 4882
Eco57I CTGAAG 7 cut(s) 728, 1371, 1556, 3654, 4085, 4522, 5816
Eco72I CACGTG 1 cut(s) 1257
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 5679
EcoNI CCTNNNNNAGG 1 cut(s) 4343
EcoO109I RGGNCCY 3 cut(s) 532, 3790, 5643
EcoRI GAATTC 2 cut(s) 3148, 5672
EcoRII CCWGG 4 cut(s) 3310, 3366, 3471, 3949
EcoRV GATATC 3 cut(s) 1648, 4593, 5477
EcoT14I CCWWGG 7 cut(s) 2024, 2347, 3994, 4108, 4464, 4516, 4698
EcoT22I ATGCAT 3 cut(s) 1329, 3709, 4899
EcoT38I GRGCYC 1 cut(s) 2257
ErhI CCWWGG 7 cut(s) 2024, 2347, 3994, 4108, 4464, 4516, 4698
Esp3I CGTCTC 2 cut(s) 370, 3262
FaqI GGGAC 5 cut(s) 1252, 1937, 3527, 5168, 5585
FauI CCCGC 2 cut(s) 267, 4351
FauNDI CATATG 6 cut(s) 1077, 1084, 1150, 1855, 3730, 5698
FbaI TGATCA 3 cut(s) 94, 604, 3922
FblI GTMKAC 3 cut(s) 1351, 2078, 5349
FriOI GRGCYC 1 cut(s) 2257
GlaI GCGC 4 cut(s) 2040, 3203, 3479, 4662
GsaI CCCAGC 1 cut(s) 1775
GsuI CTGGAG 4 cut(s) 3389, 3494, 3527, 3792
HaeII RGCGCY 2 cut(s) 3205, 4664
HaeIII GGCC 9 cut(s) 149, 533, 1321, 2644, 3329, 3555, 4884, 5644, 5798
HapII CCGG 6 cut(s) 522, 949, 1145, 2628, 2912, 3550
HgaI GACGC 2 cut(s) 3279, 4122
HhaI GCGC 4 cut(s) 2041, 3204, 3480, 4663
Hin1I GRCGYC 2 cut(s) 4114, 4522
Hin6I GCGC 4 cut(s) 2039, 3202, 3478, 4661
HinP1I GCGC 4 cut(s) 2039, 3202, 3478, 4661
HincII GTYRAC 1 cut(s) 1104
HindII GTYRAC 1 cut(s) 1104
HindIII AAGCTT 3 cut(s) 1383, 1534, 3944
HpaI GTTAAC 1 cut(s) 1104
HpaII CCGG 6 cut(s) 522, 949, 1145, 2628, 2912, 3550
HphI GGTGA 9 cut(s) 875, 1064, 2336, 2363, 3932, 4204, 4774, 5471, 5668
Hpy99I CGWCG 1 cut(s) 4383
HpyCH4IV ACGT 9 cut(s) 367, 379, 469, 1256, 1510, 1667, 2363, 4394, 4522
HpySE526I ACGT 9 cut(s) 367, 379, 469, 1256, 1510, 1667, 2363, 4394, 4522
Hsp92I GRCGYC 2 cut(s) 4114, 4522
HspAI GCGC 4 cut(s) 2039, 3202, 3478, 4661
Kpn2I TCCGGA 1 cut(s) 2911
KroI GCCGGC 1 cut(s) 1144
KroNI GCCGGC 1 cut(s) 1146
Ksp22I TGATCA 3 cut(s) 94, 604, 3922
KspAI GTTAAC 1 cut(s) 1104
LguI GCTCTTC 2 cut(s) 3519, 4029
MaeII ACGT 9 cut(s) 367, 379, 469, 1256, 1510, 1667, 2363, 4394, 4522
MaeIII GTNAC 8 cut(s) 443, 941, 1186, 1450, 3016, 3251, 3736, 4210
MfeI CAATTG 2 cut(s) 2238, 3746
MflI RGATCY 6 cut(s) 1977, 2815, 2914, 3131, 4446, 5817
MhlI GDGCHC 3 cut(s) 619, 2257, 3319
MlsI TGGCCA 1 cut(s) 3329
MluNI TGGCCA 1 cut(s) 3329
MlyI GAGTC 3 cut(s) 1175, 2334, 5770
MmeI TCCRAC 2 cut(s) 717, 5845
Mox20I TGGCCA 1 cut(s) 3329
Mph1103I ATGCAT 3 cut(s) 1329, 3709, 4899
MroI TCCGGA 1 cut(s) 2911
MroNI GCCGGC 1 cut(s) 1144
MroXI GAANNNNTTC 6 cut(s) 702, 3467, 3645, 4023, 4098, 4787
MscI TGGCCA 1 cut(s) 3329
MslI CAYNNNNRTG 7 cut(s) 102, 267, 678, 2349, 4037, 4272, 4902
Msp20I TGGCCA 1 cut(s) 3329
MspA1I CMGCKG 2 cut(s) 1244, 4831
MspI CCGG 6 cut(s) 522, 949, 1145, 2628, 2912, 3550
MspR9I CCNGG 5 cut(s) 3312, 3368, 3473, 3551, 3951
MunI CAATTG 2 cut(s) 2238, 3746
Mva1269I GAATGC 6 cut(s) 1673, 2052, 2405, 4067, 4758, 5768
MvaI CCWGG 4 cut(s) 3312, 3368, 3473, 3951
MvnI CGCG 1 cut(s) 2041
NaeI GCCGGC 1 cut(s) 1146
NciI CCSGG 1 cut(s) 3551
NcoI CCATGG 1 cut(s) 3994
NdeI CATATG 6 cut(s) 1077, 1084, 1150, 1855, 3730, 5698
NgoMIV GCCGGC 1 cut(s) 1144
NlaIV GGNNCC 7 cut(s) 201, 1953, 2256, 2537, 3339, 3554, 5645
NmuCI GTSAC 2 cut(s) 443, 4210
NsiI ATGCAT 3 cut(s) 1329, 3709, 4899
NspI RCATGY 7 cut(s) 1102, 1596, 1763, 2752, 3439, 3785, 4411
NspV TTCGAA 3 cut(s) 266, 492, 1308
OliI CACNNNNGTG 2 cut(s) 678, 2349
PaeI GCATGC 1 cut(s) 1596
PagI TCATGA 3 cut(s) 1650, 2169, 4399
PceI AGGCCT 1 cut(s) 5798
PciI ACATGT 1 cut(s) 2748
PciSI GCTCTTC 2 cut(s) 3519, 4029
PcsI WCGNNNNNNNCGW 2 cut(s) 676, 3837
PctI GAATGC 6 cut(s) 1673, 2052, 2405, 4067, 4758, 5768
PdiI GCCGGC 1 cut(s) 1146
PdmI GAANNNNTTC 6 cut(s) 702, 3467, 3645, 4023, 4098, 4787
PflMI CCANNNNNTGG 3 cut(s) 1603, 3128, 3965
PfoI TCCNGGA 1 cut(s) 3471
PleI GAGTC 3 cut(s) 1174, 2333, 5770
PmaCI CACGTG 1 cut(s) 1257
PmlI CACGTG 1 cut(s) 1257
PpsI GAGTC 3 cut(s) 1174, 2333, 5770
Ppu21I YACGTR 4 cut(s) 368, 470, 1257, 4395
PpuMI RGGWCCY 1 cut(s) 3790
PscI ACATGT 1 cut(s) 2748
PshAI GACNNNNGTC 1 cut(s) 848
PshBI ATTAAT 2 cut(s) 3378, 5075
PsiI TTATAA 1 cut(s) 1208
Psp5II RGGWCCY 1 cut(s) 3790
Psp6I CCWGG 4 cut(s) 3310, 3366, 3471, 3949
PspCI CACGTG 1 cut(s) 1257
PspFI CCCAGC 1 cut(s) 1771
PspGI CCWGG 4 cut(s) 3310, 3366, 3471, 3949
PspN4I GGNNCC 7 cut(s) 201, 1953, 2256, 2537, 3339, 3554, 5645
PspPPI RGGWCCY 1 cut(s) 3790
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 4 cut(s) 309, 874, 4686, 4830
PstNI CAGNNNCTG 1 cut(s) 3092
PsuI RGATCY 6 cut(s) 1977, 2815, 2914, 3131, 4446, 5817
PvuII CAGCTG 2 cut(s) 1244, 4831
RsaI GTAC 8 cut(s) 404, 749, 1509, 2184, 2273, 2747, 2753, 5685
RsaNI GTAC 8 cut(s) 403, 748, 1508, 2183, 2272, 2746, 2752, 5684
RseI CAYNNNNRTG 7 cut(s) 102, 267, 678, 2349, 4037, 4272, 4902
SapI GCTCTTC 2 cut(s) 3519, 4029
ScaI AGTACT 2 cut(s) 749, 5685
SchI GAGTC 3 cut(s) 1175, 2334, 5770
ScrFI CCNGG 5 cut(s) 3312, 3368, 3473, 3551, 3951
SduI GDGCHC 3 cut(s) 619, 2257, 3319
SfcI CTRYAG 7 cut(s) 305, 870, 1404, 1794, 4682, 4826, 4966
SfuI TTCGAA 3 cut(s) 266, 492, 1308
SinI GGWCC 7 cut(s) 256, 524, 1951, 3790, 3953, 5046, 5263
SmiMI CAYNNNNRTG 7 cut(s) 102, 267, 678, 2349, 4037, 4272, 4902
SmlI CTYRAG 2 cut(s) 2885, 4778
SmoI CTYRAG 2 cut(s) 2885, 4778
SpeI ACTAGT 1 cut(s) 3035
SphI GCATGC 1 cut(s) 1596
SseBI AGGCCT 1 cut(s) 5798
SsiI CCGC 7 cut(s) 223, 274, 653, 2403, 4358, 4513, 5044
SspI AATATT 3 cut(s) 817, 2413, 3112
StuI AGGCCT 1 cut(s) 5798
StyD4I CCNGG 5 cut(s) 3310, 3366, 3471, 3549, 3949
StyI CCWWGG 7 cut(s) 2024, 2347, 3994, 4108, 4464, 4516, 4698
TaiI ACGT 9 cut(s) 370, 382, 472, 1259, 1513, 1670, 2366, 4397, 4525
TaqI TCGA 8 cut(s) 189, 266, 492, 1308, 1701, 2557, 3840, 5087
TaqII GACCGA 1 cut(s) 3095
TatI WGTACW 7 cut(s) 402, 747, 2182, 2271, 2745, 2751, 5683
TauI GCSGC 2 cut(s) 225, 656
TseFI GTSAC 2 cut(s) 443, 4210
Tsp45I GTSAC 2 cut(s) 443, 4210
TspGWI ACGGA 6 cut(s) 361, 841, 1457, 2460, 3815, 5721
Van91I CCANNNNNTGG 3 cut(s) 1603, 3128, 3965
VneI GTGCAC 1 cut(s) 3315
VpaK11BI GGWCC 7 cut(s) 256, 524, 1951, 3790, 3953, 5046, 5263
VspI ATTAAT 2 cut(s) 3378, 5075
XagI CCTNNNNNAGG 1 cut(s) 4343
XbaI TCTAGA 3 cut(s) 1330, 1823, 3622
XceI RCATGY 7 cut(s) 1102, 1596, 1763, 2752, 3439, 3785, 4411
XcmI CCANNNNNNNNNTGG 1 cut(s) 2524
XmaJI CCTAGG 1 cut(s) 2024
XmiI GTMKAC 3 cut(s) 1351, 2078, 5349
XmnI GAANNNNTTC 6 cut(s) 702, 3467, 3645, 4023, 4098, 4787
ZraI GACGTC 1 cut(s) 4523
ZrmI AGTACT 2 cut(s) 749, 5685
Zsp2I ATGCAT 3 cut(s) 1329, 3709, 4899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.