RLG00000033078

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016003
Physical Location & Seq
Forward (+)
23039466 .. 23056136
16671 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033078

Sequence Viewer

Length: 5853 bp
ATGTCTGAGATTGTGGTTGCAGATCCCATCTATGATGGTCGAGCAGAGGAAAAAAGACCCATTACAAGGTATCTCACCATGGGCAGTGGGAGTGATCATAATCATCCTCCTGAGCCATTTGAGAGCGAGAGATTGCCTGCCACTTTGGCCTCAAAAATCCGGAGCTTTCTTCGGGTTGCAAACTTGATCGAAAGCGAGGAGCCTCGGATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCATTCGAATGGGAGAGGTGTTCGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACAATTAGGAAACGGAAGGAAAAGAGTGATGTACGTGAGTTAAGACGTGTTTATCATGCTTACAAGGAGTACATCATCAAACATGAAGGAGCATTTAGCATAGAAAATAGAGAGAAGTTGATAAATGCGCGTATAATTGGTTCTGTATTGTTTGAAGTCTTAAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTGGCTAATAGAGGTGGTATCCAGACAAAACCTTATGATATCTTTGGCATGCCATATAATATTCTACCACTTGATCAAGGAGGTATTCAGCAACCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAATACGGTATATTCGTGGCATACCTTCTGATGAAGATCTTCAGAAACATGGGACTTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTCTACTCCTTGCTAATATTCACAAACGGAAAACTCATAAACAGACATCTGTCTCAAAATTAGGAGATGCTGCAGTGGACGAGTTAATGCGGAGAATTTTTAAAAATTATACAAACTGGTGCAAGTTTTTGGGAAGGAAAAGCAACATCCGGTTACCTTATGTGAAACAAGAAGCCCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGCGAGGCTGCAAACTTGCGATTCATGCCGGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAGGTTATGCCAGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTTGTTGCCCCCATATATGGGGTTATAAGAGAGGAAGCTGAGAAAAGCAAGTGTGGGACAGCTGACCATTCCACATGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTCCTGATTGTTTTGAAATCGGTTGGCCCATGCGTCTAGATCACGATTTCTTCTTTACAGACTCTCCAACAAAATCCAATGCTAAGAATGCTTCAGAATCCACTGCTCCTGTAGAAGAAAGAAGGAAGGAAGATGGGGAGGAAGATGAAGTTGGGGTTACAAAAGAAGAAGTCCGTGAACCCAAATGGTTGGGCAAAACAAATTTCGTAGAAGTACGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCACTTCAGGCATTAATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAGTTGTTTGACAAAGTTATATTTGAGGATATCATGAGCATCTTCATTACATCTGCATTTCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACACTGGACTTCTCTGCAAAAGCAAAACATGTTATGAAGCTCGGTGTTGCAATGATATGGACTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATCCCACAACATACGGAAGCTGGGTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATTTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTATTCGCAAATACATTGAGGTCTCAAATTACCGAATATGCACCATTCTGTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGATTGCAAGAAAGCCAGCTTTCAGTGTTGAAGTATACATTGTTTTGGGTACTTGTACTATTGAGCAAATTTTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCCAATGTATGATTGGCATGAGCTTTTTCCAAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTCTATGGCATTTTGCATCACCTTGGTGAGATTCGAACTTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATTGAGAAATGACCAAAAGAGAAGAAAAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAAACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAGGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTGAGGACTTTAGGATGATTAAAGTTCCAGATCTTTTGGCTAAATGTATCGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCATGGCAAAGTTGTGAAAGTTCTCCAAGATATATTTGAGCTTGTAATCAATGACATGATGACGACTGGCTTCAGGATATTAGAGTTGCTGGATTCATCCCAACAGACTGAAACAGATGCTGCTTATTTTTCTGGAAGCATTGAATCCCCATTATTTGGATCTGCTGGTGGCAAGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTGAATGAGCAGATCAAGCGTTTTCTTCTGTTGCTTACCGTCCAAGATACAGCAATGGACATTCCAACTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAATATGCCACATGCACCCACAGTGGCCAATATGGTGCCATTCAGTGTTATGACACCACATTACCTGGAGGATATTAATTTTTCAATGGAGGAGCTTCATTCAAGCCAGCGAGAAGTTTCTATCATCTATTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCCTGGAGCGCATGGGTTATGAAAATTTGGATGGGTTGGAGAAGGACAAAAAGGAAGAGCTTAGAAATTGGGCTTCTTTTCGGGGCCAAACACTGAGCAGAACAGTTAGAGGAATGATGTACTATAGAGAAGCCTTAAAACTACAAGCATTTCTTGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATGCATTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTTGTTACCTGCCAATTATTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAAAAGATACCCAACTCTCCGTGTTGCTTATGTTGAGGAGAAAGAAGAGATAGTGGAGAACAAGCCTTGCAAAGTTTATTCTTCAGTATTGGTTAAAGCTATCCCCAACTATGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTGCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAACCATGGAATAATCTTCACTCGTGGTGAAGCTCTCCAAACCATTGATATGAACCAAGACAGTTATTTGGAGGAAGCCTTCAAAATGAGAAATCTTCTGCAAGAATTTCTTCAGAACCGAGGACGGCGACCTCCTATACTGCTTGGTTTGAGGGAACACATATTTACAGGAAGTGTTTCTTCTCTTGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTACGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGATTAAGCAAAGCATCAAAAACAATTAACCTGAGCGAGGATGTTTTTGCTGGGTATAATTCTACCTTACGACGGGGATGGATCACGTATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAGTTTGAAGCTAAGGTTGCTAATGGTAACAGTGAACAAACTATAAGCCGTGACATATACCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTACGGACAGCTATACCTAGTTCTCAGTGGGTTGGAAAAAGCTCTCGTAATTGAGGCTAGACTGCGGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGCTATTAACAGGAATGCCAATGGTGATGGAAATTGGACTTGAGAAAGGGTTCCTCAATGCAATAAAAGATTTTGTCCTGATGCAATTGCAGCTGGCTTCTGTTTTTTTCACGTTCTCCTTTGGAACAAAAATCCATTATTACGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTTGTTGTGTTTCATACTAGCTTCACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTCGTCAAAGGATTTGAGTTACTGCTCCTGTTGATTGTTTATAACTTGTTTAGACGGTCCTATGAGAGCGACATGGCATATGTATTAGTCACTTATTCGATTTGGTTCATGTCAATCACTTGGTTGTTCGCTCCATTTCTTTTTAATCCGTCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGATTGGAATAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCACCAAGATAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGAGTTCTAGGTTGTTTGAAATACTCCTTTCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCAGGAATTTTCTGGTTTATTTGCTTTCATGGATAGTGATCCTCGTTGTTTTCTCGTTAGTAAAGGCAGTTAACCTCGGAAGGCAAAAGTTCAGTGCTAGACATCACCTTGTGTTTAGGCTATTCAAAGCAACCCTTTTCTTGGGTGTTCTTTCCATCATAATAACTCTTTCTCTTGTGTGTCATCTATCTTGGATGGACTTGGTTGTGTGCTGCCTAGCATTTTTGCCAACAGGGTGGGGTTTGATATTGTTTGCACAAGCTGTGAGGCCCCTGATAGAGAATACTGGATTGTGGGAATTCACCAGAGTACTTGCTAAAACATATGACTACGGAATGGGGGTTGTTCTCTTTACACCTATTGCTGTCTTGGCATGGCTTCCGATTATATCAGCATTCCAGACTCGATTCCTGTTCAATGAGGCCTTCAATAGGCACTTGCAGATCCAACCAATTCTTCAGGGGAAGAAGAAGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1951

Amino Acids

225.72

Weight (kDa)

8.56

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 52 - 112 2.7e-08 Vta1 like
FKS1_dom1 PF14288 328 - 439 1.1e-41 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 881 - 1074 6.4e-48 Callose synthase 1 domain
Glucan_synthase PF02364 1081 - 1166 1.1e-24 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1171 - 1849 2.6e-194 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016003)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14570 AT3G14570 AT3G14570
fragaria_vesca FvH4_3g16960
malus_domestica MD11G1276200.v1.1
prunus_persica Prupe.4G152000_v2.0.a1
pyrus_communis pycom11g24450
rosa_chinensis RchiOBHm_Chr5g0028271
rosa_laevigata RLG00000033078
rosa_multiflora Rmu_sc0001571.1_g000003
rosa_roxburghii Rroxscaffold_1G00051450
rosa_rugosa Rorug05G0105400
rosa_samantha Rh5AG198900 Rh5DG199100
rosa_wichuraiana Rw5G018140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1205, 5028
AatII GACGTC 1 cut(s) 4522
Acc36I ACCTGC 1 cut(s) 3746
AccB1I GGYRCC 1 cut(s) 3334
AccB7I CCANNNNNTGG 4 cut(s) 1148, 1600, 3125, 3962
AccI GTMKAC 2 cut(s) 2075, 5346
AccII CGCG 2 cut(s) 463, 2038
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 6 cut(s) 223, 650, 886, 2400, 4510, 4681
AclWI GGATC 8 cut(s) 17, 2820, 3136, 3760, 4394, 5198, 5404, 5809
AcoI YGGCCR 3 cut(s) 2639, 3324, 4879
AcyI GRCGYC 1 cut(s) 4519
AdeI CACNNNGTG 1 cut(s) 5482
AflIII ACRYGT 3 cut(s) 379, 1756, 2745
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 4 cut(s) 528, 3363, 3468, 3946
AjuI GAANNNNNNNTTGG 4 cut(s) 1425, 1457, 5811, 5843
AleI CACNNNNGTG 1 cut(s) 2346
AloI GAACNNNNNNTCC 4 cut(s) 1848, 1880, 4676, 4708
Alw21I GWGCWC 1 cut(s) 3955
Alw26I GTCTC 8 cut(s) 853, 1058, 1296, 1717, 1981, 3259, 3765, 4190
Alw44I GTGCAC 1 cut(s) 3951
AlwI GGATC 8 cut(s) 17, 2820, 3136, 3760, 4394, 5198, 5404, 5809
AlwNI CAGNNNCTG 2 cut(s) 529, 3089
Aor13HI TCCGGA 1 cut(s) 159
AoxI GGCC 9 cut(s) 147, 525, 1316, 2639, 3324, 3550, 4879, 5639, 5793
ApaLI GTGCAC 1 cut(s) 3951
AseI ATTAAT 2 cut(s) 1577, 3375
Asp700I GAANNNNTTC 6 cut(s) 699, 3464, 3642, 4095, 4162, 4784
AspA2I CCTAGG 1 cut(s) 2021
AspLEI GCGC 3 cut(s) 463, 2038, 3477
AsuII TTCGAA 2 cut(s) 266, 2356
AvaII GGWCC 6 cut(s) 256, 518, 1948, 3787, 5043, 5260
AvrII CCTAGG 1 cut(s) 2021
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 3955
BalI TGGCCA 2 cut(s) 2641, 3326
BanI GGYRCC 1 cut(s) 3334
BanII GRGCYC 1 cut(s) 2254
BarI GAAGNNNNNNTAC 4 cut(s) 1816, 1848, 2753, 2785
BauI CACGAG 1 cut(s) 4008
BbsI GAAGAC 3 cut(s) 2601, 2870, 2970
Bbv12I GWGCWC 1 cut(s) 3955
BceAI ACGGC 3 cut(s) 4127, 4482, 4894
BciT130I CCWGG 4 cut(s) 530, 3365, 3470, 3948
BciVI GTATCC 1 cut(s) 557
BclI TGATCA 3 cut(s) 94, 601, 3919
BcoDI GTCTC 8 cut(s) 853, 1058, 1296, 1717, 1981, 3259, 3765, 4190
BfmI CTRYAG 6 cut(s) 305, 867, 1401, 1791, 3589, 4963
BfuAI ACCTGC 1 cut(s) 3746
BfuI GTATCC 1 cut(s) 557
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 3 cut(s) 697, 2911, 4443
BlnI CCTAGG 1 cut(s) 2021
BlpI GCTNAGC 1 cut(s) 3693
BmcAI AGTACT 2 cut(s) 746, 5682
Bme1390I CCNGG 4 cut(s) 530, 3365, 3470, 3948
Bme18I GGWCC 6 cut(s) 256, 518, 1948, 3787, 5043, 5260
BmgBI CACGTC 1 cut(s) 380
BmiI GGNNCC 8 cut(s) 201, 1950, 2253, 2534, 3336, 3551, 4787, 5642
BmrFI CCNGG 4 cut(s) 530, 3365, 3470, 3948
BmrI ACTGGG 2 cut(s) 4711, 4929
BmuI ACTGGG 2 cut(s) 4711, 4929
BoxI GACNNNNGTC 1 cut(s) 845
BpiI GAAGAC 3 cut(s) 2601, 2870, 2970
BpmI CTGGAG 3 cut(s) 3386, 3491, 3789
Bpu10I CCTNAGC 3 cut(s) 111, 4337, 4461
Bpu1102I GCTNAGC 1 cut(s) 3693
Bpu14I TTCGAA 2 cut(s) 266, 2356
BpuEI CTTGAG 2 cut(s) 2903, 4796
BsaAI YACGTR 2 cut(s) 368, 4392
BsaBI GATNNNNATC 3 cut(s) 99, 696, 5408
BsaHI GRCGYC 1 cut(s) 4519
BsaI GGTCTC 4 cut(s) 1296, 1981, 3765, 4190
BsaWI WCCGGW 2 cut(s) 159, 945
BsaXI ACNNNNNCTCC 2 cut(s) 1339, 1369
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 9 cut(s) 917, 1108, 1737, 2642, 3040, 4717, 4924, 5174, 5662
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 3 cut(s) 1701, 1785, 3240
Bse8I GATNNNNATC 3 cut(s) 99, 696, 5408
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 4 cut(s) 530, 3365, 3470, 3948
BseJI GATNNNNATC 3 cut(s) 99, 696, 5408
BseMI GCAATG 3 cut(s) 1701, 1785, 3240
BseNI ACTGG 9 cut(s) 917, 1108, 1737, 2642, 3040, 4717, 4924, 5174, 5662
BseRI GAGGAG 4 cut(s) 212, 2389, 3404, 3854
BseSI GKGCMC 1 cut(s) 3955
BseX3I CGGCCG 1 cut(s) 4879
BseYI CCCAGC 2 cut(s) 1860, 4355
Bsh1236I CGCG 2 cut(s) 463, 2038
Bsh1285I CGRYCG 1 cut(s) 4882
BshFI GGCC 9 cut(s) 149, 527, 1318, 2641, 3326, 3552, 4881, 5641, 5795
BshNI GGYRCC 1 cut(s) 3334
BsiEI CGRYCG 1 cut(s) 4882
BsiHKAI GWGCWC 1 cut(s) 3955
BsiSI CCGG 5 cut(s) 160, 516, 946, 1046, 2625
BslFI GGGAC 4 cut(s) 727, 1249, 1934, 5165
BsmAI GTCTC 8 cut(s) 853, 1058, 1296, 1717, 1981, 3259, 3765, 4190
BsmBI CGTCTC 1 cut(s) 3259
BsmFI GGGAC 4 cut(s) 727, 1249, 1934, 5165
BsmI GAATGC 4 cut(s) 1384, 2402, 4755, 5765
BsnI GGCC 9 cut(s) 149, 527, 1318, 2641, 3326, 3552, 4881, 5641, 5795
Bso31I GGTCTC 4 cut(s) 1296, 1981, 3765, 4190
Bsp119I TTCGAA 2 cut(s) 266, 2356
Bsp1286I GDGCHC 2 cut(s) 2254, 3955
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3693
Bsp19I CCATGG 2 cut(s) 78, 3991
BspACI CCGC 6 cut(s) 223, 650, 886, 2400, 4510, 4681
BspANI GGCC 9 cut(s) 149, 527, 1318, 2641, 3326, 3552, 4881, 5641, 5795
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 2 cut(s) 463, 2038
BspHI TCATGA 3 cut(s) 1647, 2166, 4396
BspLI GGNNCC 8 cut(s) 201, 1950, 2253, 2534, 3336, 3551, 4787, 5642
BspMAI CTGCAG 2 cut(s) 309, 871
BspMI ACCTGC 1 cut(s) 3746
BspPI GGATC 8 cut(s) 17, 2820, 3136, 3760, 4394, 5198, 5404, 5809
BspQI GCTCTTC 1 cut(s) 3516
BspT104I TTCGAA 2 cut(s) 266, 2356
BspT107I GGYRCC 1 cut(s) 3334
BspTNI GGTCTC 4 cut(s) 1296, 1981, 3765, 4190
BsrDI GCAATG 3 cut(s) 1701, 1785, 3240
BsrFI RCCGGY 1 cut(s) 515
BsrI ACTGG 9 cut(s) 917, 1108, 1737, 2642, 3040, 4717, 4924, 5174, 5662
BssAI RCCGGY 1 cut(s) 515
BssNAI GTATAC 1 cut(s) 2076
BssNI GRCGYC 1 cut(s) 4519
BssSI CACGAG 1 cut(s) 4008
BssT1I CCWWGG 6 cut(s) 78, 2021, 2344, 3991, 4513, 4695
Bst1107I GTATAC 1 cut(s) 2076
Bst2BI CACGAG 1 cut(s) 4008
Bst2UI CCWGG 4 cut(s) 530, 3365, 3470, 3948
Bst6I CTCTTC 3 cut(s) 1888, 3516, 3843
BstACI GRCGYC 1 cut(s) 4519
BstAPI GCANNNNNTGC 1 cut(s) 3689
BstBAI YACGTR 2 cut(s) 368, 4392
BstBI TTCGAA 2 cut(s) 266, 2356
BstC8I GCNNGC 7 cut(s) 138, 578, 1143, 1591, 2057, 3407, 4830
BstDSI CCRYGG 2 cut(s) 78, 3991
BstEII GGTNACC 1 cut(s) 948
BstENI CCTNNNNNAGG 2 cut(s) 4340, 5800
BstFNI CGCG 2 cut(s) 463, 2038
BstHHI GCGC 3 cut(s) 463, 2038, 3477
BstMAI GTCTC 8 cut(s) 853, 1058, 1296, 1717, 1981, 3259, 3765, 4190
BstMCI CGRYCG 1 cut(s) 4882
BstNI CCWGG 4 cut(s) 530, 3365, 3470, 3948
BstNSI RCATGY 9 cut(s) 580, 1099, 1593, 1760, 2749, 3314, 3436, 3782, 4408
BstPAI GACNNNNGTC 1 cut(s) 845
BstPI GGTNACC 1 cut(s) 948
BstSCI CCNGG 4 cut(s) 528, 3363, 3468, 3946
BstSFI CTRYAG 6 cut(s) 305, 867, 1401, 1791, 3589, 4963
BstSLI GKGCMC 1 cut(s) 3955
BstUI CGCG 2 cut(s) 463, 2038
BstV2I GAAGAC 3 cut(s) 2601, 2870, 2970
BstX2I RGATCY 7 cut(s) 22, 697, 2812, 2911, 3128, 4443, 5814
BstXI CCANNNNNNTGG 5 cut(s) 1480, 3456, 3749, 3992, 5607
BstYI RGATCY 7 cut(s) 22, 697, 2812, 2911, 3128, 4443, 5814
BstZ17I GTATAC 1 cut(s) 2076
BstZI CGGCCG 1 cut(s) 4879
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 557
BsuRI GGCC 9 cut(s) 149, 527, 1318, 2641, 3326, 3552, 4881, 5641, 5795
BtgI CCRYGG 2 cut(s) 78, 3991
BtrI CACGTC 1 cut(s) 380
BtsI GCAGTG 3 cut(s) 91, 876, 1392
BveI ACCTGC 1 cut(s) 3746
Cac8I GCNNGC 7 cut(s) 138, 578, 1143, 1591, 2057, 3407, 4830
CaiI CAGNNNCTG 2 cut(s) 529, 3089
CciI TCATGA 3 cut(s) 1647, 2166, 4396
CfoI GCGC 3 cut(s) 463, 2038, 3477
Cfr10I RCCGGY 1 cut(s) 515
CseI GACGC 2 cut(s) 1313, 3276
CspCI CAANNNNNGTGG 4 cut(s) 1599, 1634, 2412, 2447
DraI TTTAAA 1 cut(s) 898
DraIII CACNNNGTG 1 cut(s) 5482
EaeI YGGCCR 3 cut(s) 2639, 3324, 4879
EagI CGGCCG 1 cut(s) 4879
Eam1104I CTCTTC 3 cut(s) 1888, 3516, 3843
EarI CTCTTC 3 cut(s) 1888, 3516, 3843
EclXI CGGCCG 1 cut(s) 4879
Eco130I CCWWGG 6 cut(s) 78, 2021, 2344, 3991, 4513, 4695
Eco147I AGGCCT 1 cut(s) 5795
Eco24I GRGCYC 1 cut(s) 2254
Eco31I GGTCTC 4 cut(s) 1296, 1981, 3765, 4190
Eco32I GATATC 3 cut(s) 568, 1645, 4590
Eco47I GGWCC 6 cut(s) 256, 518, 1948, 3787, 5043, 5260
Eco52I CGGCCG 1 cut(s) 4879
Eco81I CCTNAGG 1 cut(s) 522
Eco91I GGTNACC 1 cut(s) 948
EcoNI CCTNNNNNAGG 2 cut(s) 4340, 5800
EcoO109I RGGNCCY 3 cut(s) 526, 3787, 5640
EcoO65I GGTNACC 1 cut(s) 948
EcoRI GAATTC 2 cut(s) 3145, 5669
EcoRII CCWGG 4 cut(s) 528, 3363, 3468, 3946
EcoRV GATATC 3 cut(s) 568, 1645, 4590
EcoT14I CCWWGG 6 cut(s) 78, 2021, 2344, 3991, 4513, 4695
EcoT22I ATGCAT 2 cut(s) 3685, 3706
EcoT38I GRGCYC 1 cut(s) 2254
ErhI CCWWGG 6 cut(s) 78, 2021, 2344, 3991, 4513, 4695
Esp3I CGTCTC 1 cut(s) 3259
FaqI GGGAC 4 cut(s) 727, 1249, 1934, 5165
FauNDI CATATG 6 cut(s) 1074, 1081, 1147, 3727, 5065, 5695
FbaI TGATCA 3 cut(s) 94, 601, 3919
FblI GTMKAC 2 cut(s) 2075, 5346
FriOI GRGCYC 1 cut(s) 2254
GlaI GCGC 3 cut(s) 462, 2037, 3476
GsaI CCCAGC 2 cut(s) 1864, 4359
GsuI CTGGAG 3 cut(s) 3386, 3491, 3789
HaeIII GGCC 9 cut(s) 149, 527, 1318, 2641, 3326, 3552, 4881, 5641, 5795
HapII CCGG 5 cut(s) 160, 516, 946, 1046, 2625
HgaI GACGC 2 cut(s) 1313, 3276
HhaI GCGC 3 cut(s) 463, 2038, 3477
Hin1I GRCGYC 1 cut(s) 4519
Hin6I GCGC 3 cut(s) 461, 2036, 3475
HinP1I GCGC 3 cut(s) 461, 2036, 3475
HincII GTYRAC 2 cut(s) 1101, 5443
HindII GTYRAC 2 cut(s) 1101, 5443
HindIII AAGCTT 2 cut(s) 1531, 3941
HpaI GTTAAC 2 cut(s) 1101, 5443
HpaII CCGG 5 cut(s) 160, 516, 946, 1046, 2625
Hpy99I CGWCG 1 cut(s) 4380
HpyCH4IV ACGT 6 cut(s) 367, 379, 1507, 4391, 4519, 4847
HpySE526I ACGT 6 cut(s) 367, 379, 1507, 4391, 4519, 4847
Hsp92I GRCGYC 1 cut(s) 4519
HspAI GCGC 3 cut(s) 461, 2036, 3475
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 3 cut(s) 94, 601, 3919
KspAI GTTAAC 2 cut(s) 1101, 5443
LguI GCTCTTC 1 cut(s) 3516
MaeII ACGT 6 cut(s) 367, 379, 1507, 4391, 4519, 4847
MaeIII GTNAC 8 cut(s) 948, 1447, 3733, 4207, 4475, 4499, 5004, 5074
MfeI CAATTG 1 cut(s) 4820
MflI RGATCY 7 cut(s) 22, 697, 2812, 2911, 3128, 4443, 5814
MhlI GDGCHC 2 cut(s) 2254, 3955
MlsI TGGCCA 2 cut(s) 2641, 3326
MluNI TGGCCA 2 cut(s) 2641, 3326
MlyI GAGTC 4 cut(s) 1172, 1346, 2331, 5767
MmeI TCCRAC 5 cut(s) 1382, 3269, 3483, 4629, 5842
Mox20I TGGCCA 2 cut(s) 2641, 3326
Mph1103I ATGCAT 2 cut(s) 3685, 3706
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 6 cut(s) 699, 3464, 3642, 4095, 4162, 4784
MscI TGGCCA 2 cut(s) 2641, 3326
MslI CAYNNNNRTG 5 cut(s) 267, 2346, 4034, 4269, 4899
Msp20I TGGCCA 2 cut(s) 2641, 3326
MspA1I CMGCKG 2 cut(s) 1241, 4828
MspI CCGG 5 cut(s) 160, 516, 946, 1046, 2625
MspR9I CCNGG 4 cut(s) 530, 3365, 3470, 3948
MunI CAATTG 1 cut(s) 4820
Mva1269I GAATGC 4 cut(s) 1384, 2402, 4755, 5765
MvaI CCWGG 4 cut(s) 530, 3365, 3470, 3948
MvnI CGCG 2 cut(s) 463, 2038
NcoI CCATGG 2 cut(s) 78, 3991
NdeI CATATG 6 cut(s) 1074, 1081, 1147, 3727, 5065, 5695
NlaIV GGNNCC 8 cut(s) 201, 1950, 2253, 2534, 3336, 3551, 4787, 5642
NmuCI GTSAC 3 cut(s) 4207, 4499, 5074
NsiI ATGCAT 2 cut(s) 3685, 3706
NspI RCATGY 9 cut(s) 580, 1099, 1593, 1760, 2749, 3314, 3436, 3782, 4408
NspV TTCGAA 2 cut(s) 266, 2356
OliI CACNNNNGTG 1 cut(s) 2346
PaeI GCATGC 2 cut(s) 580, 1593
PagI TCATGA 3 cut(s) 1647, 2166, 4396
PceI AGGCCT 1 cut(s) 5795
PciI ACATGT 2 cut(s) 1756, 2745
PciSI GCTCTTC 1 cut(s) 3516
PcsI WCGNNNNNNNCGW 1 cut(s) 673
PctI GAATGC 4 cut(s) 1384, 2402, 4755, 5765
PdmI GAANNNNTTC 6 cut(s) 699, 3464, 3642, 4095, 4162, 4784
PflMI CCANNNNNTGG 4 cut(s) 1148, 1600, 3125, 3962
PfoI TCCNGGA 1 cut(s) 3468
PleI GAGTC 4 cut(s) 1171, 1346, 2330, 5767
PpsI GAGTC 4 cut(s) 1171, 1346, 2330, 5767
Ppu21I YACGTR 2 cut(s) 368, 4392
PpuMI RGGWCCY 1 cut(s) 3787
PscI ACATGT 2 cut(s) 1756, 2745
PshAI GACNNNNGTC 1 cut(s) 845
PshBI ATTAAT 2 cut(s) 1577, 3375
PsiI TTATAA 2 cut(s) 1205, 5028
Psp5II RGGWCCY 1 cut(s) 3787
Psp6I CCWGG 4 cut(s) 528, 3363, 3468, 3946
PspEI GGTNACC 1 cut(s) 948
PspFI CCCAGC 2 cut(s) 1860, 4355
PspGI CCWGG 4 cut(s) 528, 3363, 3468, 3946
PspN4I GGNNCC 8 cut(s) 201, 1950, 2253, 2534, 3336, 3551, 4787, 5642
PspPPI RGGWCCY 1 cut(s) 3787
PsrI GAACNNNNNNTAC 2 cut(s) 457, 489
PstI CTGCAG 2 cut(s) 309, 871
PstNI CAGNNNCTG 2 cut(s) 529, 3089
PsuI RGATCY 7 cut(s) 22, 697, 2812, 2911, 3128, 4443, 5814
PvuII CAGCTG 2 cut(s) 1241, 4828
RseI CAYNNNNRTG 5 cut(s) 267, 2346, 4034, 4269, 4899
SapI GCTCTTC 1 cut(s) 3516
ScaI AGTACT 2 cut(s) 746, 5682
SchI GAGTC 4 cut(s) 1172, 1346, 2331, 5767
ScrFI CCNGG 4 cut(s) 530, 3365, 3470, 3948
SduI GDGCHC 2 cut(s) 2254, 3955
SfcI CTRYAG 6 cut(s) 305, 867, 1401, 1791, 3589, 4963
SfuI TTCGAA 2 cut(s) 266, 2356
SinI GGWCC 6 cut(s) 256, 518, 1948, 3787, 5043, 5260
SmiMI CAYNNNNRTG 5 cut(s) 267, 2346, 4034, 4269, 4899
SmlI CTYRAG 2 cut(s) 2882, 4775
SmoI CTYRAG 2 cut(s) 2882, 4775
SphI GCATGC 2 cut(s) 580, 1593
SseBI AGGCCT 1 cut(s) 5795
SsiI CCGC 6 cut(s) 223, 650, 886, 2400, 4510, 4681
SspI AATATT 3 cut(s) 589, 814, 2410
StuI AGGCCT 1 cut(s) 5795
StyD4I CCNGG 4 cut(s) 528, 3363, 3468, 3946
StyI CCWWGG 6 cut(s) 78, 2021, 2344, 3991, 4513, 4695
TaiI ACGT 6 cut(s) 370, 382, 1510, 4394, 4522, 4850
TatI WGTACW 9 cut(s) 402, 744, 2095, 2268, 2742, 2748, 2784, 3585, 5680
TauI GCSGC 2 cut(s) 225, 653
TseFI GTSAC 3 cut(s) 4207, 4499, 5074
Tsp45I GTSAC 3 cut(s) 4207, 4499, 5074
TspGWI ACGGA 8 cut(s) 361, 838, 1454, 1869, 3812, 4635, 5124, 5718
Van91I CCANNNNNTGG 4 cut(s) 1148, 1600, 3125, 3962
VneI GTGCAC 1 cut(s) 3951
VpaK11BI GGWCC 6 cut(s) 256, 518, 1948, 3787, 5043, 5260
VspI ATTAAT 2 cut(s) 1577, 3375
XagI CCTNNNNNAGG 2 cut(s) 4340, 5800
XbaI TCTAGA 2 cut(s) 1327, 1820
XceI RCATGY 9 cut(s) 580, 1099, 1593, 1760, 2749, 3314, 3436, 3782, 4408
XcmI CCANNNNNNNNNTGG 2 cut(s) 2190, 2521
XmaJI CCTAGG 1 cut(s) 2021
XmiI GTMKAC 2 cut(s) 2075, 5346
XmnI GAANNNNTTC 6 cut(s) 699, 3464, 3642, 4095, 4162, 4784
ZraI GACGTC 1 cut(s) 4520
ZrmI AGTACT 2 cut(s) 746, 5682
Zsp2I ATGCAT 2 cut(s) 3685, 3706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.