AT3G20020
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009348
Physical Location & Seq
Reverse (-)
6983702 .. 6988101
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20020.2

Sequence Viewer

Length: 1308 bp
ATGCAATCTGGCGGCGATTTCAGCAACGGTTTCCACGGAGACCATCACCGCGAGCTTGAATTGGAAGACAAGCAAGGCCCCAGTCTCAGCTCTTTTGGCCGAGCCAAGAAGCGCAGTCACGCCGGAGCTCGTGACCCTCGAGGCGGTCTCGCCAATGTGCTTAGGGTTTCTGACCAGCTTGGCGAGCACAAGTCATTGGAGACTTCGGAATCTTCTCCTCCGCCTTGTACTGACTTCGACGTTGCCTATTTTCATTCCTATGCTCACGTTGGGATTCACGAAGAGATGATCAAGGATCGGGCAAGGACAGAAACTTATAGGGAAGCCATAATGCAGCATCAGAGCTTAATCGAAGGCAAGGTCGTGGTGGACGTTGGCTGTGGAACTGGCATCCTTTCAATCTTCTGTGCTCAAGCAGGTGCAAAACGGGTATATGCTGTAGATGCAAGTGATATTGCTGTCCAGGCAAAGGAAGTTGTGAAAGCCAACGGTTTATCCGATAAGGTCATTGTCTTGCACGGGAGAGTGGAGGATGTTGAAATCGACGAGGAGGTTGATGTTATAATTTCAGAGTGGATGGGTTACATGCTCTTGTATGAGAGCATGCTGGGAAGTGTTATCACAGCTAGAGATCGCTGGTTGAAGCCTGGAGGTTTAATTCTCCCATCACATGCAACATTATACATGGCACCTATTTCTCACCCTGACAGATACAGTCACAGTATTGATTTTTGGCGCAATGTTTATGGAATTGATATGTCTGCGATGATGCAATTAGCTAAACAGTGTGCTTTTGAAGAACCCAGCGTGGAGTCCATATCAGGCGAGAACGTTCTAACATGGCCAGAAGTGGTTAAACATATAGACTGTAAAACTATAAAAATCCAAGAGCTAGACTCGGTTACTGCAAGATATAAATTCAACTCAATGATGAGAGCTCCAATGCATGGTTTTGCATTTTGGTTCGACGTTGAATTCAGCGGACCGGCCTCTTCACCTGCCAAGAATACTAGTGAAACCAGTATTGCTAGTGGTTCTTCGTCGATAAGTCCTTCTGGAGAAGTAAATCAGAAGAAGCGGACTAATCCGAGTGATGCACTTGTGTTGTCCACCTCTCCCGAGTCTCCTCCAACGCATTGGCAGCAAACGATTGTATATTTCTATGACCCTATAGACGTAGAGCAAGACCAAGTCATTGAAGGTTCCGTGACACTTTCTCAAAGCAAAGAGAACAAGAGATTCATGAACATCCACCTCGAATATTCCTCGGCCGGCCGTTCCTTTGTAAAAGAGTCAGTAATGCGTTAA

Protein Analysis

435

Amino Acids

48.27

Weight (kDa)

5.52

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 115 - 169 6.1e-06 Methyltransferase domain
PrmA PF06325 118 - 155 7.4e-09 Ribosomal protein L11 methyltransferase (PrmA)
PRMT_C PF22528 202 - 306 1.5e-21 Arginine methyltransferase oligomerization subdomain
PRMT_C PF22528 343 - 401 1.2e-14 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 563
AarI CACCTGC 2 cut(s) 407, 1006
AbsI CCTCGAGG 1 cut(s) 138
Acc36I ACCTGC 2 cut(s) 407, 1006
AccB1I GGYRCC 1 cut(s) 688
AccB7I CCANNNNNTGG 1 cut(s) 947
AccII CGCG 1 cut(s) 51
AciI CCGC 6 cut(s) 12, 49, 144, 221, 981, 1078
AclI AACGTT 1 cut(s) 831
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 4 cut(s) 97, 842, 1269, 1273
AcsI RAATTY 2 cut(s) 917, 974
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 3 cut(s) 143, 469, 947
AgsI TTSAA 8 cut(s) 59, 399, 539, 643, 797, 922, 974, 1199
AhlI ACTAGT 1 cut(s) 1010
AjnI CCWGG 2 cut(s) 462, 646
AluBI AGCT 9 cut(s) 55, 90, 128, 178, 345, 626, 779, 892, 938
AluI AGCT 9 cut(s) 55, 90, 128, 178, 345, 626, 779, 892, 938
Alw21I GWGCWC 4 cut(s) 130, 189, 412, 940
Alw26I GTCTC 5 cut(s) 33, 89, 152, 194, 1128
AlwI GGATC 1 cut(s) 303
Ama87I CYCGRG 2 cut(s) 138, 1118
AoxI GGCC 6 cut(s) 76, 97, 842, 987, 1269, 1273
ApeKI GCWGC 2 cut(s) 334, 1141
ApoI RAATTY 2 cut(s) 917, 974
ArsI GACNNNNNNTTYG 2 cut(s) 345, 377
AspLEI GCGC 2 cut(s) 114, 738
AspS9I GGNCC 2 cut(s) 77, 983
AsuHPI GGTGA 3 cut(s) 38, 692, 987
AvaI CYCGRG 2 cut(s) 138, 1118
AvaII GGWCC 1 cut(s) 983
BalI TGGCCA 1 cut(s) 844
BanI GGYRCC 1 cut(s) 688
BanII GRGCYC 2 cut(s) 130, 940
BauI CACGAG 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 4 cut(s) 130, 189, 412, 940
BbvI GCAGC 2 cut(s) 346, 1153
BccI CCATC 3 cut(s) 51, 571, 673
BceAI ACGGC 1 cut(s) 1260
BciT130I CCWGG 2 cut(s) 464, 648
BclI TGATCA 1 cut(s) 288
BcoDI GTCTC 5 cut(s) 33, 89, 152, 194, 1128
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 4 cut(s) 627, 893, 1011, 1029
BfmI CTRYAG 2 cut(s) 438, 1170
BfuAI ACCTGC 2 cut(s) 407, 1006
BisI GCNGC 3 cut(s) 13, 335, 1142
BlsI GCNGC 3 cut(s) 14, 336, 1143
Bme1390I CCNGG 2 cut(s) 464, 648
Bme18I GGWCC 1 cut(s) 983
BmeT110I CYCGRG 2 cut(s) 138, 1118
BmgT120I GGNCC 2 cut(s) 77, 983
BmiI GGNNCC 3 cut(s) 79, 690, 1204
BmrFI CCNGG 2 cut(s) 464, 648
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 5 cut(s) 346, 399, 433, 759, 1084
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 4 cut(s) 132, 164, 881, 913
BpmI CTGGAG 2 cut(s) 669, 1077
Bpu10I CCTNAGC 1 cut(s) 161
BpuEI CTTGAG 1 cut(s) 396
BsaI GGTCTC 2 cut(s) 33, 152
BsaJI CCNNGG 2 cut(s) 34, 1266
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 469, 947
Bse118I RCCGGY 2 cut(s) 985, 1271
Bse1I ACTGG 3 cut(s) 81, 391, 1020
Bse3DI GCAATG 1 cut(s) 745
BseBI CCWGG 2 cut(s) 464, 648
BseDI CCNNGG 2 cut(s) 34, 1266
BseGI GGATG 4 cut(s) 390, 538, 582, 1248
BseLI CCNNNNNNNGG 3 cut(s) 143, 469, 947
BseMI GCAATG 1 cut(s) 745
BseMII CTCAG 1 cut(s) 100
BseNI ACTGG 3 cut(s) 81, 391, 1020
BseRI GAGGAG 3 cut(s) 207, 563, 1116
BseX3I CGGCCG 2 cut(s) 1269, 1273
BseXI GCAGC 2 cut(s) 346, 1153
BseYI CCCAGC 2 cut(s) 607, 803
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 2 cut(s) 1272, 1276
BshFI GGCC 6 cut(s) 78, 99, 844, 989, 1271, 1275
BshNI GGYRCC 1 cut(s) 688
BsiEI CGRYCG 2 cut(s) 1272, 1276
BsiHKAI GWGCWC 4 cut(s) 130, 189, 412, 940
BsiHKCI CYCGRG 2 cut(s) 138, 1118
BsiSI CCGG 3 cut(s) 123, 986, 1272
BslI CCNNNNNNNGG 3 cut(s) 143, 469, 947
BsmAI GTCTC 5 cut(s) 33, 89, 152, 194, 1128
BsnI GGCC 6 cut(s) 78, 99, 844, 989, 1271, 1275
Bso31I GGTCTC 2 cut(s) 33, 152
BsoBI CYCGRG 2 cut(s) 138, 1118
Bsp1286I GDGCHC 4 cut(s) 130, 189, 412, 940
Bsp143I GATC 3 cut(s) 288, 295, 631
BspACI CCGC 6 cut(s) 12, 49, 144, 221, 981, 1078
BspANI GGCC 6 cut(s) 78, 99, 844, 989, 1271, 1275
BspCNI CTCAG 1 cut(s) 99
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 1242
BspLI GGNNCC 3 cut(s) 79, 690, 1204
BspMI ACCTGC 2 cut(s) 407, 1006
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 688
BspTNI GGTCTC 2 cut(s) 33, 152
BsrDI GCAATG 1 cut(s) 745
BsrFI RCCGGY 2 cut(s) 985, 1271
BsrI ACTGG 3 cut(s) 81, 391, 1020
BssAI RCCGGY 2 cut(s) 985, 1271
BssECI CCNNGG 2 cut(s) 34, 1266
BssMI GATC 3 cut(s) 288, 295, 631
BssSI CACGAG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 464, 648
Bst4CI ACNGT 6 cut(s) 29, 491, 716, 722, 786, 869
Bst6I CTCTTC 2 cut(s) 276, 997
BstC8I GCNNGC 4 cut(s) 53, 185, 605, 1273
BstDEI CTNAG 2 cut(s) 86, 161
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 4 cut(s) 390, 538, 582, 1248
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 2 cut(s) 114, 738
BstKTI GATC 3 cut(s) 291, 298, 634
BstMAI GTCTC 5 cut(s) 33, 89, 152, 194, 1128
BstMBI GATC 3 cut(s) 288, 295, 631
BstMCI CGRYCG 2 cut(s) 1272, 1276
BstMWI GCNNNNNNNGC 6 cut(s) 21, 96, 184, 443, 464, 1141
BstNI CCWGG 2 cut(s) 464, 648
BstNSI RCATGY 3 cut(s) 589, 607, 674
BstSCI CCNGG 2 cut(s) 462, 646
BstSFI CTRYAG 2 cut(s) 438, 1170
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 346, 1153
BstV2I GAAGAC 1 cut(s) 72
BstXI CCANNNNNNTGG 1 cut(s) 1137
BstZI CGGCCG 2 cut(s) 1269, 1273
BsuRI GGCC 6 cut(s) 78, 99, 844, 989, 1271, 1275
BtgI CCRYGG 1 cut(s) 34
BtgZI GCGATG 1 cut(s) 779
BtsCI GGATG 4 cut(s) 390, 538, 582, 1248
BtsIMutI CAGTG 1 cut(s) 791
BveI ACCTGC 2 cut(s) 407, 1006
Cac8I GCNNGC 4 cut(s) 53, 185, 605, 1273
CciI TCATGA 1 cut(s) 1242
CfoI GCGC 2 cut(s) 114, 738
Cfr10I RCCGGY 2 cut(s) 985, 1271
Cfr13I GGNCC 2 cut(s) 77, 983
CpoI CGGWCCG 1 cut(s) 983
Csp6I GTAC 1 cut(s) 228
CspI CGGWCCG 1 cut(s) 983
CviAII CATG 7 cut(s) 586, 604, 671, 685, 840, 947, 1243
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 2 cut(s) 86, 161
DpnI GATC 3 cut(s) 290, 297, 633
DpnII GATC 3 cut(s) 288, 295, 631
EaeI YGGCCR 4 cut(s) 97, 842, 1269, 1273
EagI CGGCCG 2 cut(s) 1269, 1273
Eam1104I CTCTTC 2 cut(s) 276, 997
EarI CTCTTC 2 cut(s) 276, 997
EciI GGCGGA 1 cut(s) 210
Ecl136II GAGCTC 2 cut(s) 128, 938
EclXI CGGCCG 2 cut(s) 1269, 1273
Eco24I GRGCYC 2 cut(s) 130, 940
Eco31I GGTCTC 2 cut(s) 33, 152
Eco47I GGWCC 1 cut(s) 983
Eco52I CGGCCG 2 cut(s) 1269, 1273
Eco53kI GAGCTC 2 cut(s) 128, 938
Eco88I CYCGRG 2 cut(s) 138, 1118
EcoICRI GAGCTC 2 cut(s) 128, 938
EcoO109I RGGNCCY 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 974
EcoRII CCWGG 2 cut(s) 462, 646
EcoT22I ATGCAT 1 cut(s) 948
EcoT38I GRGCYC 2 cut(s) 130, 940
FaeI CATG 7 cut(s) 589, 607, 674, 688, 843, 950, 1246
FatI CATG 7 cut(s) 585, 603, 670, 684, 839, 946, 1242
FbaI TGATCA 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 13, 335, 1142
FokI GGATG 4 cut(s) 377, 545, 589, 1235
FriOI GRGCYC 2 cut(s) 130, 940
FseI GGCCGGCC 1 cut(s) 1275
Fsp4HI GCNGC 3 cut(s) 13, 335, 1142
FspBI CTAG 4 cut(s) 627, 893, 1011, 1029
GlaI GCGC 2 cut(s) 113, 737
GluI GCNGC 3 cut(s) 13, 335, 1142
GsaI CCCAGC 2 cut(s) 611, 807
GsuI CTGGAG 2 cut(s) 669, 1077
HaeIII GGCC 6 cut(s) 78, 99, 844, 989, 1271, 1275
HapII CCGG 3 cut(s) 123, 986, 1272
HhaI GCGC 2 cut(s) 114, 738
Hin1II CATG 7 cut(s) 589, 607, 674, 688, 843, 950, 1246
Hin6I GCGC 2 cut(s) 112, 736
HinP1I GCGC 2 cut(s) 112, 736
HinfI GANTC 7 cut(s) 209, 274, 812, 896, 1121, 1239, 1292
HpaII CCGG 3 cut(s) 123, 986, 1272
HphI GGTGA 3 cut(s) 38, 692, 987
Hpy166II GTNNAC 2 cut(s) 370, 1110
Hpy188I TCNGA 7 cut(s) 172, 208, 342, 499, 571, 1071, 1089
Hpy188III TCNNGA 5 cut(s) 131, 278, 1056, 1118, 1243
Hpy8I GTNNAC 2 cut(s) 370, 1110
Hpy99I CGWCG 4 cut(s) 242, 548, 971, 1045
HpyAV CCTTC 3 cut(s) 347, 1062, 1193
HpyCH4III ACNGT 6 cut(s) 29, 491, 716, 722, 786, 869
HpyCH4IV ACGT 6 cut(s) 240, 267, 372, 831, 969, 1176
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 96, 184, 443, 464, 1141
HpyF3I CTNAG 2 cut(s) 86, 161
HpySE526I ACGT 6 cut(s) 240, 267, 372, 831, 969, 1176
Hsp92II CATG 7 cut(s) 589, 607, 674, 688, 843, 950, 1246
HspAI GCGC 2 cut(s) 112, 736
KroI GCCGGC 1 cut(s) 1271
KroNI GCCGGC 1 cut(s) 1273
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 3 cut(s) 288, 295, 631
LmnI GCTCC 2 cut(s) 125, 943
Lsp1109I GCAGC 2 cut(s) 346, 1153
LweI GCATC 5 cut(s) 346, 399, 433, 759, 1084
MaeI CTAG 4 cut(s) 627, 893, 1011, 1029
MaeII ACGT 6 cut(s) 240, 267, 372, 831, 969, 1176
MaeIII GTNAC 6 cut(s) 116, 131, 581, 716, 901, 1207
MalI GATC 3 cut(s) 290, 297, 633
MboI GATC 3 cut(s) 288, 295, 631
MboII GAAGA 8 cut(s) 77, 204, 293, 394, 809, 984, 1029, 1084
MhlI GDGCHC 4 cut(s) 130, 189, 412, 940
MlsI TGGCCA 1 cut(s) 844
MluCI AATT 7 cut(s) 59, 564, 657, 750, 773, 917, 974
MluNI TGGCCA 1 cut(s) 844
MlyI GAGTC 4 cut(s) 821, 890, 1130, 1301
MmeI TCCRAC 1 cut(s) 1154
Mox20I TGGCCA 1 cut(s) 844
Mph1103I ATGCAT 1 cut(s) 948
MroNI GCCGGC 1 cut(s) 1271
MscI TGGCCA 1 cut(s) 844
MseI TTAA 4 cut(s) 347, 656, 855, 1306
MslI CAYNNNNRTG 1 cut(s) 258
Msp20I TGGCCA 1 cut(s) 844
MspA1I CMGCKG 1 cut(s) 981
MspI CCGG 3 cut(s) 123, 986, 1272
MspR9I CCNGG 2 cut(s) 464, 648
MvaI CCWGG 2 cut(s) 464, 648
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 6 cut(s) 21, 96, 184, 443, 464, 1141
NaeI GCCGGC 1 cut(s) 1273
NdeII GATC 3 cut(s) 288, 295, 631
NgoMIV GCCGGC 1 cut(s) 1271
NlaIII CATG 7 cut(s) 589, 607, 674, 688, 843, 950, 1246
NlaIV GGNNCC 3 cut(s) 79, 690, 1204
NmeAIII GCCGAG 2 cut(s) 125, 1247
NmuCI GTSAC 4 cut(s) 116, 131, 716, 1207
NsiI ATGCAT 1 cut(s) 948
NspI RCATGY 3 cut(s) 589, 607, 674
PaeI GCATGC 1 cut(s) 607
PaeR7I CTCGAG 1 cut(s) 138
PagI TCATGA 1 cut(s) 1242
PaqCI CACCTGC 2 cut(s) 407, 1006
PcsI WCGNNNNNNNCGW 3 cut(s) 136, 369, 495
PdiI GCCGGC 1 cut(s) 1273
PfeI GAWTC 3 cut(s) 209, 274, 1239
PflFI GACNNNGTC 1 cut(s) 1190
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 3 cut(s) 14, 336, 1143
PleI GAGTC 4 cut(s) 820, 890, 1129, 1300
PpsI GAGTC 4 cut(s) 820, 890, 1129, 1300
PsiI TTATAA 1 cut(s) 563
Psp124BI GAGCTC 2 cut(s) 130, 940
Psp1406I AACGTT 1 cut(s) 831
Psp6I CCWGG 2 cut(s) 462, 646
PspFI CCCAGC 2 cut(s) 607, 803
PspGI CCWGG 2 cut(s) 462, 646
PspN4I GGNNCC 3 cut(s) 79, 690, 1204
PspPI GGNCC 2 cut(s) 77, 983
PspXI VCTCGAGB 1 cut(s) 138
PsyI GACNNNGTC 1 cut(s) 1190
RigI GGCCGGCC 1 cut(s) 1275
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 258
Rsr2I CGGWCCG 1 cut(s) 983
RsrII CGGWCCG 1 cut(s) 983
SacI GAGCTC 2 cut(s) 130, 940
SaqAI TTAA 4 cut(s) 347, 656, 855, 1306
SatI GCNGC 3 cut(s) 13, 335, 1142
Sau3AI GATC 3 cut(s) 288, 295, 631
Sau96I GGNCC 2 cut(s) 77, 983
SchI GAGTC 4 cut(s) 821, 890, 1130, 1301
ScrFI CCNGG 2 cut(s) 464, 648
SduI GDGCHC 4 cut(s) 130, 189, 412, 940
SfaNI GCATC 5 cut(s) 346, 399, 433, 759, 1084
SfcI CTRYAG 2 cut(s) 438, 1170
Sfr274I CTCGAG 1 cut(s) 138
SinI GGWCC 1 cut(s) 983
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 2 cut(s) 138, 411
SmoI CTYRAG 2 cut(s) 138, 411
SpeI ACTAGT 1 cut(s) 1010
SphI GCATGC 1 cut(s) 607
Sse9I AATT 7 cut(s) 59, 564, 657, 750, 773, 917, 974
SsiI CCGC 6 cut(s) 12, 49, 144, 221, 981, 1078
SspI AATATT 1 cut(s) 1262
SspMI CTAG 4 cut(s) 627, 893, 1011, 1029
SstI GAGCTC 2 cut(s) 130, 940
StyD4I CCNGG 2 cut(s) 462, 646
TaaI ACNGT 6 cut(s) 29, 491, 716, 722, 786, 869
TaiI ACGT 6 cut(s) 243, 270, 375, 834, 972, 1179
TaqI TCGA 7 cut(s) 139, 237, 351, 543, 966, 1043, 1257
TasI AATT 7 cut(s) 59, 564, 657, 750, 773, 917, 974
TatI WGTACW 1 cut(s) 227
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 3 cut(s) 209, 274, 1239
Tru1I TTAA 4 cut(s) 347, 656, 855, 1306
Tru9I TTAA 4 cut(s) 347, 656, 855, 1306
TscAI CASTG 1 cut(s) 791
TseFI GTSAC 4 cut(s) 116, 131, 716, 1207
TseI GCWGC 2 cut(s) 334, 1141
Tsp45I GTSAC 4 cut(s) 116, 131, 716, 1207
TspDTI ATGAA 3 cut(s) 242, 1231, 1259
TspGWI ACGGA 2 cut(s) 51, 1195
TspRI CASTG 1 cut(s) 791
Tth111I GACNNNGTC 1 cut(s) 1190
Van91I CCANNNNNTGG 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 983
XapI RAATTY 2 cut(s) 917, 974
XceI RCATGY 3 cut(s) 589, 607, 674
XhoI CTCGAG 1 cut(s) 138
XspI CTAG 4 cut(s) 627, 893, 1011, 1029
Zsp2I ATGCAT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.