Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004599

Physical Location & Seq

Genomic Coordinates Reverse (-)

7058871 .. 7063372

Gene Structure 4502 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G20250.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGACGACTACGCAGAGCGCTATGAGAATGGTTGAGGGTGATCATATAAAAAATTGGCAGGCTTCTAGTGATAGTGGGATCTTTGGTTCACTTGATATGGCTGTTGAAGATTTAGGATTCCTTATGAAAAGAAATAGGTTGGATAGTGGTGATCAAACCGGTAAATTCCCTAGTAGAAGCGAAAGCGCGCCGCCTAGCATGGAAGGTTCCTTTGCAGCTCTTAGGAATCTCTTGAAACAACAAGAAGGTAGTAGCTCTGAAGTTTTAAGTAGGGCTATTGAGAATTATGACTCGGAAGAAGAGATACGTAGTGATCCTGCTTATGTAGCTTACTATTTGTCGAACATCAACTTGAATCCGAGACTCCCTCCTCCGTTAATCTCACGGGAGAATCAGCACTTGTTGCGTCATTTTGGTGATAATAATCAGAGTCCAACAACTTCTTGGGATAATATGGGGATAAGATCATCTTTGCATTCTTCTAGAACTGCTCTTTCAACTCATAGAGAGGAGCCCGAGGATGAGGCATCATCGGGTGAACAACAATCTTATGCTTCTTTGGCTGGTCGTCGTAAGAGCATAGCTGATATGATTCAGCGGCCTCATTCGGCAGGAAATCGTCCAATAGCACAAGACATCCATGCCATTTCCTCTGATACATCTTCAGAACACGCAAGAAGATTACCGGAGTCCGATATAAATTCGGTTAATCTGCTGAGGGAGACAGATTCTCTCTCCAGCGATGCTATTGCTAGTGAAGATCCCTTTACCACTGATTTGGCCTCACAAAGTTTTACTAATGCACAGACAGAGAGATTGAACGCGAGGCAAGCGAGCCATGAGGACAACAATCTATCTGTTTTTGGTGCTTCTCCTCCATCCTCTGTTGCTTCAAGAATGAGGAGGAACCAAGAAGACCAACAATCTCAGGGAAGGAGAATGCCTCCACAATACACACCTTCATCGTATCAGGTTCAAGCTAGTTCGCCACAGCAAATGAGCTATCCGAGGATAGGTGGTACACAGGACATGATGCAGAGCTTACCAAAGATTGCAACTGGCGAGTCCTCGGGTATGTTCGTCCCGCAATATAATTATGGTGGTTACCCTCCTGCTTCTGGTATTGTCCCTCAATATATGAGTGGATACCCATCTCATGAAGCGACTGTTCCTATGCCATATGATATCTCATCTACTTCTTCGGGCTACAACAACCCTCGGCTCCTGCCTGGAGTATCATCGAGTGGACAAAACATTCCTTCTCTTGTGGATCCTTTTCAGTTGCAATATTTTCAACAGGCTCAAGTGGACGCGTATGCTCCTCCGTTTCAGAGCAGCACTGATTCTTTTGGGCAGAAAGACCAACAAGCTGTTGGCTATATGGCAAATCATGAACCTCTCAACAGTCCATTGAGTCCGGGTTACGGGTTGCAAAGTCCGCGGCACATGGGAAACTATTTTGCAGTGCCGCCTGGTGTAAGAGTAATGCCGCAGTATCCAGGATCACCTCTTGCTAGTCCGGTGATGCCATCCTCACCGGTTGGTGGAATGATGAGCCACTTTGGAAGACGAAGTGAAACAAGGTATCATCAACAAGGGCCAAGTAGAAACACTGGGATCTACCCTGGTGGATGGCAAGGGAACAGAGGGGGAGCCAGCAGCATCGTTGATGATCTAAAAAGACATTCTTTTCTTGATGAACTTAAGTCTCCAAATGCTCGGAAACTCGAGCTTTCTGATATTGCAGGGCGTGTTGTTGAATTCAGCGTCGATCAGCATGGCAGCCGGTTTATTCAACAGAAGTTGGAGCACTGCTCCGATGAGGAGAAGGCATCTGTTTTCAGTGAGGTTCTTCCACAAGCTTCAAAATTGATGACTGACGTTTTTGGAAATTATGTCATCCAAAAATTCATAGAACATGGAACTCCGGCACAGAGAGAAGAACTTGTGAAGCAACTTGCTGGTCAGATGGTTTCACTAAGCTTACAAATGTATGGATGCCGTGTGATACAAAAGGCCCTTGAAGTGATAGATGTTGACCAAAAGACAGAACTAATTCGTGAGCTCGATGGGAATGTGCTGAAGTGTGTTAGAGATCAAAACGGAAATCATGTTATCCAAAAATGTATCGAGAGTATGCCTGCTGGTAGGATTGGATTCGTAATTGCTGCTTTCCGTGGTCAAGTCGCCACTCTTTCTACTCATCCTTATGGATGCCGAGTTATCCAGAGAATCTTGGAGCATTGCTCAGATGATGAGGAGACTCATTGTATAATAGATGAAATATTGGAATCTGCGTTTGCTCTTGCTCATGATCAGTATGGGAATTATGTCACTCAGCATGTCCTGGAGAGAGGGAAGCCCGATGAAAGGAGACAGATCATTGAGAAGTTGACAGGGAATGTTGTTCAGATGAGTCAGCACAAATACGCATCTAACGTTGTAGAGAAGTGTTTGGAACACGCTGATAGTACCGAAAGAGAGTTCCTGATTGAAGAGATAATGGGCAAATCCGAGGAAGACAATCACTTGCTAGCGATGATGAAGGATCAGTTTGCAAATTACGTGGTCCAGAAAGTCTTGGAGATCAGTAAAGATCAGCAAAGGGAGATTCTGGTGCAGAGGATGAAAATTCATCTCCAAAGTTTGCGGAAATACACATACGGGAAACACATAGTAGCCCGATTCGAACAACTGTTTGGTGAAGAGAGTGAAGTCTCAGAAGAGGGAACAGAAGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NABP PUF

Protein Analysis

913

Amino Acids

101.6

Weight (kDa)

5.57

Isoelectric Point (pI)

64.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	620 - 654	1.6e-12	Pumilio-family RNA binding repeat
PUF	PF00806	659 - 689	2.3e-09	Pumilio-family RNA binding repeat
PUF	PF00806	692 - 725	2.9e-09	Pumilio-family RNA binding repeat
PUF	PF00806	764 - 799	1e-13	Pumilio-family RNA binding repeat
PUF_NOP9	PF22493	801 - 872	3.2e-06	NOP9-like PUF repeat domain
PUF	PF00806	803 - 835	1.4e-07	Pumilio-family RNA binding repeat
PUF	PF00806	837 - 871	1.4e-11	Pumilio-family RNA binding repeat
PUF	PF00806	879 - 910	6.1e-07	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20250 AT3G20250 AT3G20250
fragaria_vesca	FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910
malus_domestica	MD09G1165700.v1.1 MD17G1161900.v1.1
prunus_persica	Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1
pyrus_communis	pycom07g18900 pycom09g08300 pycom17g15500
rosa_chinensis	RchiOBHm_Chr2g0149341
rosa_laevigata	RLG00000020394
rosa_multiflora	Rmu_sc0000873.1_g000017
rosa_roxburghii	Rroxscaffold_2G00098200
rosa_rugosa	Rorug02G0410600 Rorug02G0410700 Rorug02G0410800
rosa_samantha	Rh2AG471400 Rh2BG483600 Rh2CG457300 Rh2DG492700
rosa_wichuraiana	Rw2G038360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	4 cut(s)	188, 824, 1313, 1441
AciI	CCGC	8 cut(s)	191, 598, 1085, 1439, 1441, 1469, 1490, 2650
AclI	AACGTT	1 cut(s)	2439
AclWI	GGATC	8 cut(s)	86, 308, 755, 1265, 1278, 1510, 1625, 2556
AcsI	RAATTY	5 cut(s)	164, 700, 1760, 1907, 2631
AcuI	CTGAAG	3 cut(s)	279, 648, 2102
AfaI	GTAC	2 cut(s)	1021, 2473
AfeI	AGCGCT	1 cut(s)	19
AfiI	CCNNNNNNNGG	5 cut(s)	1013, 1118, 1424, 1544, 2370
AflII	CTTAAG	1 cut(s)	1703
AflIII	ACRYGT	1 cut(s)	1311
AgeI	ACCGGT	2 cut(s)	158, 1537
AjnI	CCWGG	5 cut(s)	1228, 1471, 1498, 1624, 2346
AjuI	GAANNNNNNNTTGG	2 cut(s)	2682, 2714
AloI	GAACNNNNNNTCC	2 cut(s)	70, 102
Alw21I	GWGCWC	2 cut(s)	1812, 2067
Alw26I	GTCTC	6 cut(s)	355, 716, 1713, 2255, 2368, 2722
AlwI	GGATC	8 cut(s)	86, 308, 755, 1265, 1278, 1510, 1625, 2556
Ama87I	CYCGRG	3 cut(s)	515, 1069, 1727
Aor51HI	AGCGCT	1 cut(s)	19
AoxI	GGCC	4 cut(s)	599, 780, 1598, 2016
ApeKI	GCWGC	5 cut(s)	215, 1335, 1659, 1782, 2168
ApoI	RAATTY	5 cut(s)	164, 700, 1760, 1907, 2631
AsiGI	ACCGGT	2 cut(s)	158, 1537
Asp700I	GAANNNNTTC	1 cut(s)	2055
AspLEI	GCGC	3 cut(s)	20, 188, 190
AspS9I	GGNCC	3 cut(s)	1598, 2017, 2569
AsuC2I	CCSGG	1 cut(s)	1419
AsuHPI	GGTGA	8 cut(s)	50, 161, 428, 548, 1497, 1527, 1534, 2714
AsuII	TTCGAA	1 cut(s)	2688
AsuNHI	GCTAGC	1 cut(s)	2533
AvaI	CYCGRG	3 cut(s)	515, 1069, 1727
AvaII	GGWCC	1 cut(s)	2569
BamHI	GGATCC	1 cut(s)	1270
BanII	GRGCYC	2 cut(s)	516, 2067
BarI	GAAGNNNNNNTAC	2 cut(s)	288, 320
BbsI	GAAGAC	3 cut(s)	921, 1573, 2526
Bbv12I	GWGCWC	2 cut(s)	1812, 2067
BbvCI	CCTCAGC	1 cut(s)	716
BbvI	GCAGC	5 cut(s)	227, 1347, 1671, 1794, 2155
BccI	CCATC	6 cut(s)	886, 1159, 1537, 1626, 1963, 2063
BceAI	ACGGC	1 cut(s)	1986
BcgI	CGANNNNNNTGC	2 cut(s)	731, 765
BciT130I	CCWGG	5 cut(s)	1230, 1473, 1500, 1626, 2348
BciVI	GTATCC	2 cut(s)	1139, 1506
BclI	TGATCA	3 cut(s)	40, 151, 2314
BcnI	CCSGG	1 cut(s)	1419
BcoDI	GTCTC	6 cut(s)	355, 716, 1713, 2255, 2368, 2722
BfaI	CTAG	8 cut(s)	66, 171, 195, 483, 753, 981, 1515, 2534
BfoI	RGCGCY	1 cut(s)	21
BfrI	CTTAAG	1 cut(s)	1703
BfuI	GTATCC	2 cut(s)	1139, 1506
Bme1390I	CCNGG	6 cut(s)	1230, 1419, 1473, 1500, 1626, 2348
Bme18I	GGWCC	1 cut(s)	2569
BmeT110I	CYCGRG	3 cut(s)	515, 1069, 1727
BmgT120I	GGNCC	3 cut(s)	1598, 2017, 2569
BmiI	GGNNCC	6 cut(s)	208, 513, 908, 1223, 1272, 1654
BmrFI	CCNGG	6 cut(s)	1230, 1419, 1473, 1500, 1626, 2348
BmrI	ACTGGG	1 cut(s)	1623
BmsI	GCATC	9 cut(s)	536, 733, 1023, 1515, 1671, 1841, 1988, 2204, 2441
BmtI	GCTAGC	1 cut(s)	2537
BmuI	ACTGGG	1 cut(s)	1623
BpiI	GAAGAC	3 cut(s)	921, 1573, 2526
BplI	GAGNNNNNCTC	8 cut(s)	352, 384, 928, 960, 1799, 1831, 2231, 2263
BpmI	CTGGAG	3 cut(s)	721, 1251, 2369
Bpu10I	CCTNAGC	1 cut(s)	716
Bpu14I	TTCGAA	1 cut(s)	2688
BpuEI	CTTGAG	1 cut(s)	1287
BpuMI	CCSGG	1 cut(s)	1419
BsaAI	YACGTR	2 cut(s)	308, 2566
BsaBI	GATNNNNATC	2 cut(s)	423, 1150
BsaJI	CCNNGG	8 cut(s)	516, 1007, 1068, 1217, 1439, 1624, 2176, 2514
BsaWI	WCCGGW	4 cut(s)	158, 685, 1519, 1537
Bsc4I	CCNNNNNNNGG	5 cut(s)	1013, 1118, 1424, 1544, 2370
Bse118I	RCCGGY	3 cut(s)	158, 1537, 1785
Bse1I	ACTGG	2 cut(s)	1063, 1618
Bse3DI	GCAATG	1 cut(s)	2242
Bse8I	GATNNNNATC	2 cut(s)	423, 1150
BseBI	CCWGG	5 cut(s)	1230, 1473, 1500, 1626, 2348
BseDI	CCNNGG	8 cut(s)	516, 1007, 1068, 1217, 1439, 1624, 2176, 2514
BseJI	GATNNNNATC	2 cut(s)	423, 1150
BseLI	CCNNNNNNNGG	5 cut(s)	1013, 1118, 1424, 1544, 2370
BseMI	GCAATG	1 cut(s)	2242
BseMII	CTCAG	5 cut(s)	707, 941, 2262, 2351, 2733
BseNI	ACTGG	2 cut(s)	1063, 1618
BsePI	GCGCGC	1 cut(s)	186
BseRI	GAGGAG	7 cut(s)	360, 524, 864, 916, 1311, 1838, 2273
BseXI	GCAGC	5 cut(s)	227, 1347, 1671, 1794, 2155
BsgI	GTGCAG	1 cut(s)	2639
Bsh1236I	CGCG	4 cut(s)	188, 824, 1313, 1441
BshFI	GGCC	4 cut(s)	601, 782, 1600, 2018
BshTI	ACCGGT	2 cut(s)	158, 1537
BsiHKAI	GWGCWC	2 cut(s)	1812, 2067
BsiHKCI	CYCGRG	3 cut(s)	515, 1069, 1727
BsiSI	CCGG	7 cut(s)	159, 686, 1418, 1520, 1538, 1786, 1928
BslFI	GGGAC	2 cut(s)	1067, 1112
BslI	CCNNNNNNNGG	5 cut(s)	1013, 1118, 1424, 1544, 2370
BsmAI	GTCTC	6 cut(s)	355, 716, 1713, 2255, 2368, 2722
BsmFI	GGGAC	2 cut(s)	1067, 1112
BsmI	GAATGC	2 cut(s)	475, 945
BsnI	GGCC	4 cut(s)	601, 782, 1600, 2018
BsoBI	CYCGRG	3 cut(s)	515, 1069, 1727
Bsp119I	TTCGAA	1 cut(s)	2688
Bsp1286I	GDGCHC	3 cut(s)	516, 1812, 2067
BspACI	CCGC	8 cut(s)	191, 598, 1085, 1439, 1441, 1469, 1490, 2650
BspANI	GGCC	4 cut(s)	601, 782, 1600, 2018
BspCNI	CTCAG	5 cut(s)	708, 940, 2261, 2350, 2732
BspFNI	CGCG	4 cut(s)	188, 824, 1313, 1441
BspHI	TCATGA	3 cut(s)	1156, 1390, 2311
BspLI	GGNNCC	6 cut(s)	208, 513, 908, 1223, 1272, 1654
BspOI	GCTAGC	1 cut(s)	2537
BspPI	GGATC	8 cut(s)	86, 308, 755, 1265, 1278, 1510, 1625, 2556
BspT104I	TTCGAA	1 cut(s)	2688
BspTI	CTTAAG	1 cut(s)	1703
BsrDI	GCAATG	1 cut(s)	2242
BsrFI	RCCGGY	3 cut(s)	158, 1537, 1785
BsrI	ACTGG	2 cut(s)	1063, 1618
BssAI	RCCGGY	3 cut(s)	158, 1537, 1785
BssECI	CCNNGG	8 cut(s)	516, 1007, 1068, 1217, 1439, 1624, 2176, 2514
BssHII	GCGCGC	1 cut(s)	186
Bst2UI	CCWGG	5 cut(s)	1230, 1473, 1500, 1626, 2348
Bst4CI	ACNGT	3 cut(s)	1168, 1406, 2697
Bst6I	CTCTTC	4 cut(s)	294, 2490, 2700, 2718
BstAFI	CTTAAG	1 cut(s)	1703
BstAPI	GCANNNNNTGC	1 cut(s)	403
BstBAI	YACGTR	2 cut(s)	308, 2566
BstBI	TTCGAA	1 cut(s)	2688
BstC8I	GCNNGC	7 cut(s)	60, 188, 831, 835, 1657, 2142, 2535
BstDEI	CTNAG	7 cut(s)	221, 716, 927, 1979, 2248, 2337, 2719
BstDSI	CCRYGG	2 cut(s)	1439, 2176
BstEII	GGTNACC	1 cut(s)	1103
BstFNI	CGCG	4 cut(s)	188, 824, 1313, 1441
BstH2I	RGCGCY	1 cut(s)	21
BstHHI	GCGC	3 cut(s)	20, 188, 190
BstMAI	GTCTC	6 cut(s)	355, 716, 1713, 2255, 2368, 2722
BstMWI	GCNNNNNNNGC	5 cut(s)	326, 403, 560, 830, 1438
BstNI	CCWGG	5 cut(s)	1230, 1473, 1500, 1626, 2348
BstNSI	RCATGY	1 cut(s)	2345
BstPI	GGTNACC	1 cut(s)	1103
BstSCI	CCNGG	6 cut(s)	1228, 1417, 1471, 1498, 1624, 2346
BstSNI	TACGTA	1 cut(s)	308
BstUI	CGCG	4 cut(s)	188, 824, 1313, 1441
BstV1I	GCAGC	5 cut(s)	227, 1347, 1671, 1794, 2155
BstV2I	GAAGAC	3 cut(s)	921, 1573, 2526
BstX2I	RGATCY	4 cut(s)	78, 760, 1270, 1617
BstXI	CCANNNNNNTGG	1 cut(s)	778
BstYI	RGATCY	4 cut(s)	78, 760, 1270, 1617
BsuI	GTATCC	2 cut(s)	1139, 1506
BsuRI	GGCC	4 cut(s)	601, 782, 1600, 2018
BtgI	CCRYGG	2 cut(s)	1439, 2176
BtgZI	GCGATG	2 cut(s)	756, 2552
BtsI	GCAGTG	2 cut(s)	1470, 1810
BtsIMutI	CAGTG	6 cut(s)	771, 1338, 1470, 1611, 1810, 1849
Cac8I	GCNNGC	7 cut(s)	60, 188, 831, 835, 1657, 2142, 2535
CciI	TCATGA	3 cut(s)	1156, 1390, 2311
CfoI	GCGC	3 cut(s)	20, 188, 190
Cfr10I	RCCGGY	3 cut(s)	158, 1537, 1785
Cfr13I	GGNCC	3 cut(s)	1598, 2017, 2569
Cfr42I	CCGCGG	1 cut(s)	1442
CseI	GACGC	3 cut(s)	395, 1319, 1756
Csp6I	GTAC	2 cut(s)	1020, 2472
CspAI	ACCGGT	2 cut(s)	158, 1537
CspCI	CAANNNNNGTGG	2 cut(s)	2547, 2582
CviQI	GTAC	2 cut(s)	1020, 2472
DdeI	CTNAG	7 cut(s)	221, 716, 927, 1979, 2248, 2337, 2719
Eam1104I	CTCTTC	4 cut(s)	294, 2490, 2700, 2718
EarI	CTCTTC	4 cut(s)	294, 2490, 2700, 2718
Ecl136II	GAGCTC	1 cut(s)	2065
Eco105I	TACGTA	1 cut(s)	308
Eco24I	GRGCYC	2 cut(s)	516, 2067
Eco32I	GATATC	1 cut(s)	1186
Eco47I	GGWCC	1 cut(s)	2569
Eco47III	AGCGCT	1 cut(s)	19
Eco53kI	GAGCTC	1 cut(s)	2065
Eco57I	CTGAAG	3 cut(s)	279, 648, 2102
Eco88I	CYCGRG	3 cut(s)	515, 1069, 1727
Eco91I	GGTNACC	1 cut(s)	1103
EcoICRI	GAGCTC	1 cut(s)	2065
EcoO109I	RGGNCCY	1 cut(s)	2017
EcoO65I	GGTNACC	1 cut(s)	1103
EcoRI	GAATTC	1 cut(s)	1760
EcoRII	CCWGG	5 cut(s)	1228, 1471, 1498, 1624, 2346
EcoRV	GATATC	1 cut(s)	1186
EcoT38I	GRGCYC	2 cut(s)	516, 2067
FalI	AAGNNNNNCTT	4 cut(s)	454, 486, 1672, 1704
FaqI	GGGAC	2 cut(s)	1067, 1112
FauI	CCCGC	1 cut(s)	1092
FauNDI	CATATG	1 cut(s)	1180
FbaI	TGATCA	3 cut(s)	40, 151, 2314
FriOI	GRGCYC	2 cut(s)	516, 2067
FspBI	CTAG	8 cut(s)	66, 171, 195, 483, 753, 981, 1515, 2534
GlaI	GCGC	3 cut(s)	19, 187, 189
GsuI	CTGGAG	3 cut(s)	721, 1251, 2369
HaeII	RGCGCY	1 cut(s)	21
HaeIII	GGCC	4 cut(s)	601, 782, 1600, 2018
HapII	CCGG	7 cut(s)	159, 686, 1418, 1520, 1538, 1786, 1928
HgaI	GACGC	3 cut(s)	395, 1319, 1756
HhaI	GCGC	3 cut(s)	20, 188, 190
Hin6I	GCGC	3 cut(s)	18, 186, 188
HinP1I	GCGC	3 cut(s)	18, 186, 188
HincII	GTYRAC	2 cut(s)	2038, 2394
HindII	GTYRAC	2 cut(s)	2038, 2394
HindIII	AAGCTT	2 cut(s)	1860, 1981
HpaII	CCGG	7 cut(s)	159, 686, 1418, 1520, 1538, 1786, 1928
HphI	GGTGA	8 cut(s)	50, 161, 428, 548, 1497, 1527, 1534, 2714
Hpy166II	GTNNAC	7 cut(s)	89, 539, 1022, 1247, 1309, 2038, 2394
Hpy8I	GTNNAC	7 cut(s)	89, 539, 1022, 1247, 1309, 2038, 2394
Hpy99I	CGWCG	2 cut(s)	573, 1772
HpyAV	CCTTC	8 cut(s)	197, 239, 927, 969, 1269, 1822, 2539, 2729
HpyCH4III	ACNGT	3 cut(s)	1168, 1406, 2697
HpyCH4IV	ACGT	4 cut(s)	307, 1881, 2439, 2565
HpyF10VI	GCNNNNNNNGC	5 cut(s)	326, 403, 560, 830, 1438
HpyF3I	CTNAG	7 cut(s)	221, 716, 927, 1979, 2248, 2337, 2719
HpySE526I	ACGT	4 cut(s)	307, 1881, 2439, 2565
HspAI	GCGC	3 cut(s)	18, 186, 188
Ksp22I	TGATCA	3 cut(s)	40, 151, 2314
KspI	CCGCGG	1 cut(s)	1442
LmnI	GCTCC	7 cut(s)	511, 1227, 1324, 1652, 1807, 1820, 2239
Lsp1109I	GCAGC	5 cut(s)	227, 1347, 1671, 1794, 2155
LweI	GCATC	9 cut(s)	536, 733, 1023, 1515, 1671, 1841, 1988, 2204, 2441
MaeI	CTAG	8 cut(s)	66, 171, 195, 483, 753, 981, 1515, 2534
MaeII	ACGT	4 cut(s)	307, 1881, 2439, 2565
MaeIII	GTNAC	3 cut(s)	1103, 1421, 2332
MflI	RGATCY	4 cut(s)	78, 760, 1270, 1617
MhlI	GDGCHC	3 cut(s)	516, 1812, 2067
MluI	ACGCGT	1 cut(s)	1311
MlyI	GAGTC	8 cut(s)	284, 357, 439, 698, 1073, 1423, 2257, 2425
MmeI	TCCRAC	3 cut(s)	120, 458, 1785
MroXI	GAANNNNTTC	1 cut(s)	2055
MseI	TTAA	4 cut(s)	266, 377, 708, 1704
MslI	CAYNNNNRTG	2 cut(s)	414, 2208
MspA1I	CMGCKG	2 cut(s)	598, 1441
MspCI	CTTAAG	1 cut(s)	1703
MspI	CCGG	7 cut(s)	159, 686, 1418, 1520, 1538, 1786, 1928
MspR9I	CCNGG	6 cut(s)	1230, 1419, 1473, 1500, 1626, 2348
Mva1269I	GAATGC	2 cut(s)	475, 945
MvaI	CCWGG	5 cut(s)	1230, 1473, 1500, 1626, 2348
MvnI	CGCG	4 cut(s)	188, 824, 1313, 1441
MwoI	GCNNNNNNNGC	5 cut(s)	326, 403, 560, 830, 1438
NciI	CCSGG	1 cut(s)	1419
NdeI	CATATG	1 cut(s)	1180
NheI	GCTAGC	1 cut(s)	2533
NlaIV	GGNNCC	6 cut(s)	208, 513, 908, 1223, 1272, 1654
NmeAIII	GCCGAG	2 cut(s)	1198, 2243
NmuCI	GTSAC	1 cut(s)	2332
NspI	RCATGY	1 cut(s)	2345
NspV	TTCGAA	1 cut(s)	2688
PaeR7I	CTCGAG	1 cut(s)	1727
PagI	TCATGA	3 cut(s)	1156, 1390, 2311
PauI	GCGCGC	1 cut(s)	186
PcsI	WCGNNNNNNNCGW	1 cut(s)	2436
PctI	GAATGC	2 cut(s)	475, 945
PdmI	GAANNNNTTC	1 cut(s)	2055
PfoI	TCCNGGA	2 cut(s)	1498, 2346
PinAI	ACCGGT	2 cut(s)	158, 1537
PleI	GAGTC	8 cut(s)	284, 357, 438, 697, 1072, 1422, 2257, 2424
PpsI	GAGTC	8 cut(s)	284, 357, 438, 697, 1072, 1422, 2257, 2424
Ppu21I	YACGTR	2 cut(s)	308, 2566
Psp124BI	GAGCTC	1 cut(s)	2067
Psp1406I	AACGTT	1 cut(s)	2439
Psp6I	CCWGG	5 cut(s)	1228, 1471, 1498, 1624, 2346
PspEI	GGTNACC	1 cut(s)	1103
PspGI	CCWGG	5 cut(s)	1228, 1471, 1498, 1624, 2346
PspN4I	GGNNCC	6 cut(s)	208, 513, 908, 1223, 1272, 1654
PspPI	GGNCC	3 cut(s)	1598, 2017, 2569
PspXI	VCTCGAGB	1 cut(s)	1727
PsuI	RGATCY	4 cut(s)	78, 760, 1270, 1617
PteI	GCGCGC	1 cut(s)	186
RsaI	GTAC	2 cut(s)	1021, 2473
RsaNI	GTAC	2 cut(s)	1020, 2472
RseI	CAYNNNNRTG	2 cut(s)	414, 2208
SacI	GAGCTC	1 cut(s)	2067
SacII	CCGCGG	1 cut(s)	1442
SaqAI	TTAA	4 cut(s)	266, 377, 708, 1704
Sau96I	GGNCC	3 cut(s)	1598, 2017, 2569
SchI	GAGTC	8 cut(s)	284, 357, 439, 698, 1073, 1423, 2257, 2425
ScrFI	CCNGG	6 cut(s)	1230, 1419, 1473, 1500, 1626, 2348
SduI	GDGCHC	3 cut(s)	516, 1812, 2067
SfaNI	GCATC	9 cut(s)	536, 733, 1023, 1515, 1671, 1841, 1988, 2204, 2441
Sfr274I	CTCGAG	1 cut(s)	1727
Sfr303I	CCGCGG	1 cut(s)	1442
SfuI	TTCGAA	1 cut(s)	2688
SgrBI	CCGCGG	1 cut(s)	1442
SinI	GGWCC	1 cut(s)	2569
SlaI	CTCGAG	1 cut(s)	1727
SmiMI	CAYNNNNRTG	2 cut(s)	414, 2208
SmlI	CTYRAG	3 cut(s)	1302, 1703, 1727
SmoI	CTYRAG	3 cut(s)	1302, 1703, 1727
SnaBI	TACGTA	1 cut(s)	308
SsiI	CCGC	8 cut(s)	191, 598, 1085, 1439, 1441, 1469, 1490, 2650
SspI	AATATT	2 cut(s)	1289, 2286
SspMI	CTAG	8 cut(s)	66, 171, 195, 483, 753, 981, 1515, 2534
SstI	GAGCTC	1 cut(s)	2067
StyD4I	CCNGG	6 cut(s)	1228, 1417, 1471, 1498, 1624, 2346
TaaI	ACNGT	3 cut(s)	1168, 1406, 2697
TaiI	ACGT	4 cut(s)	310, 1884, 2442, 2568
TaqI	TCGA	7 cut(s)	341, 1241, 1728, 1770, 2067, 2130, 2688
TauI	GCSGC	5 cut(s)	193, 601, 1444, 1471, 1492
Tru1I	TTAA	4 cut(s)	266, 377, 708, 1704
Tru9I	TTAA	4 cut(s)	266, 377, 708, 1704
TscAI	CASTG	6 cut(s)	778, 1345, 1470, 1618, 1817, 1849
TseFI	GTSAC	1 cut(s)	2332
TseI	GCWGC	5 cut(s)	215, 1335, 1659, 1782, 2168
Tsp45I	GTSAC	1 cut(s)	2332
TspGWI	ACGGA	4 cut(s)	363, 1314, 2118, 2165
TspRI	CASTG	6 cut(s)	778, 1345, 1470, 1618, 1817, 1849
Vha464I	CTTAAG	1 cut(s)	1703
VpaK11BI	GGWCC	1 cut(s)	2569
XapI	RAATTY	5 cut(s)	164, 700, 1760, 1907, 2631
XbaI	TCTAGA	1 cut(s)	482
XceI	RCATGY	1 cut(s)	2345
XcmI	CCANNNNNNNNNTGG	2 cut(s)	441, 1370
XhoI	CTCGAG	1 cut(s)	1727
XmnI	GAANNNNTTC	1 cut(s)	2055
XspI	CTAG	8 cut(s)	66, 171, 195, 483, 753, 981, 1515, 2534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.