Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0004599

Physical Location & Seq

Genomic Coordinates Reverse (-)

71325359 .. 71332715

Gene Structure 7357 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG492700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2655 bp

ATGGCAACGGAGAGTCTCATGAGAACGGTGGAAAGCCGTAAAAGTGAAAAGTGGCATTCATCGATGGATGCGGCAACATATGGATCTCCACTGAAAAGTACGGCAGCAGAGGAATTGGGCTTCCTTACAAAGGGGCACAGGTTTCAGAGAGATAGAGCTGAGGCCATACCTAATCGAAGTGGTAGTGCTCCCCCAAGTATGGAGGGTTCCTTTTACGCTATTGGGAACCTTTTAAGTCAACAGAACTCCAGTATGATAACAAGCTCGACTAATTCAAGGAACACAGTTCAAAATGTTCAGTCTGAGGAACTGTATTCTGACCCATCTTATTTGGCATACTGCTTCGCTAATATGAACTTAAATTTAAGGCCTCCTCCACGCCATCCTGTTGGTTCCAGCACCAATTGGAGATTGACTTCTTTAGACGACAGTGGCAACGGATCCTTCCATCTTTCTCAAGGTTCTCTTTCCACTCACAAGGAGGAGCTTGATGATGCTAGTTCATCCAAGCAAGCTTCAGATAATTTGGCAGAAAATAGTGTTTCAGCAATGGCGGTGAAGAACACTCATTCTTTGGCAAGCCATAATAAGAGTGTGCTGGATCGCATACAGGAAGACTTCCCCCGAACTCCATCTCCTGTGTATAATCAATCTGTCTCATCAAGTATTGCAACAGATGAACCGGTTGATATTGATGTACATTCGATTTCACCAAATGCTTCTTCTGCTAGCATACGACAATTACAGTCCTCAAATTCAGGCTCTATCGACATATATCCGGAGACAAGTTCCTTGACTACCTCTTCTCCTAGGACACTACATCCTAATGAGACAGGGAACTTACATGAAGATGAATCAAATATTGAGGATACTGGGGTGGGGAGTAATGGATCAATTGGTGGAGCCCTTGGGTTAGATCTTTCCCCAACTGGGTCTAGAATTAGAGCATCTAATATTAACAAACAACATGAGAAACACTCTTATGGGACGGGTGTGCTACAACACCTTTTGTCTACACAACAAGGGTTACCATATCAACTTCAGGCTGTTCAGGACCATGTAGTTTCACAAGGCATGAATCATTGGCAAAGTAGAATGGATCCCCATGGCTATCCAAAGTTCTCTTCCATTGAGGTACAGCCATCATTGCAATCACCTGGATTCACACCTCCCTTGTATGCTACAACAGCAGGGTATATGACTTCAGGAAATGCTTTCTACCCCAATTTTCAGCCGTCCAGTATATATCCTGCACAATATGGTGCGAGTGGATATACCATGGGTTCTTCATTTCTTCCCCCATATATGGCTGCGTACCATTCTCATGGTTCCTTTCCTCTGCCTTTTGATGCTACTTTGGGTCAAAGCTTAAATGGTCGAACTGCAGGTGTTTCAACAGGGGAGAGAAGTCCCCATGAAGGTGATTTGCATCACCTAAGCAAGTTTTACGGCCAGCCTGGGCCAATGCTCCAGCCATCTTTTCTTGATCCCCTAAGTATGCAATACTATCCACGTCCCTTGGATGATTCATACAGTGCTTCAATTCAGTATGGTCAATTAGGTCCAAGGGGTATTATTGGAGGCCAGCTTTACCAACAAGAGTCAAATGTTACTGCATATGCAGGTGACCAGAAATTTCAGTCTCCAACTAATGGGTCTCTGGGCATTCCAAGTCCAAGAAAAATGGGAATCAATGGTAGTGGTTATTATGGTAGTCCTTCTAGCATGGGTGGCATGACATTTCCAGCCTCACCTCTTGGTAGTCCTATACCACCGTCATCTCCAGTAGGCAGAACACATCACCATGGTCGGCCAAATGAATCGAGGTATCACCAAGGTTCGATAAGAAATGGTGGACTATATTCTGGGTGGCAAGGACAGAGAAGCTTTAACAACTTTGAGGACTCTAAAAGGCACTCCTTTCTTGAAGAACTGAAATCCAGTAATGCCCGTAAATTTGAACTCTCTGATATAGCAGGGCGCATTGCTGAATTCAGTGTGGATCAACATGGGAGTCGATTTATTCAGCAGAAGCTTGAATACTGCAGTGTCGAAGACAAGGCGTCTGTTTTCAAAGAGGTTCTTCCACATGCTTCAAAATTAATGACTGATGTATTTGGGAATTATGTTATTCAAAAGTTTTTTGAGTATGGGACTCCTGAGCAGAAAAAGGAGCTTGCAGATAAACTCAGTGGACAGATGTTCCCTTTAAGTTTGCAGATGTATGGTTGTCGTGTGATCCAGAAGGCTCTTGAAGTTATAGAGGTTGACCAGAAGACACAACTTGTGCATGAGCTTGATGGACATGTTATGAAATGTGTGCATGATCAAAATGGAAATCATGTAATACAGAAGTGTATAGAATGCATTCCTACAGAGAAAATTGGATTCATCATATCTTCTTTCCGAGGAGAAGTTGCCAAACTTTCTACTCATCCATATGGTTGCCGTGTCATACAGAGAGTTTTGGAGCATTGTTCAGATGAGCTTCAAGGTCAGTGTGTTGTGGATGAGATTTTGGGATCTGCTTATGTTCTTGCTCAGAACCAGTATGGCAATTATGTCACCCAGGTTGGTTTTCTTACTTTCTGCTCTCTGTTGCTTCCTTTATCTAGGCCACATAACTATTATTTTCTGTTTATTTCTGAAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NABP PUF

Protein Analysis

884

Amino Acids

96.93

Weight (kDa)

6.16

Isoelectric Point (pI)

49.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NABP	PF07990	404 - 656	1.3e-13	Nucleic acid binding protein NABP
PUF	PF00806	662 - 695	7.2e-11	Pumilio-family RNA binding repeat
PUF	PF00806	699 - 730	3e-09	Pumilio-family RNA binding repeat
PUF	PF00806	735 - 766	1.1e-08	Pumilio-family RNA binding repeat
PUF	PF00806	806 - 840	5e-12	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20250 AT3G20250 AT3G20250
fragaria_vesca	FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910
malus_domestica	MD09G1165700.v1.1 MD17G1161900.v1.1
prunus_persica	Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1
pyrus_communis	pycom07g18900 pycom09g08300 pycom17g15500
rosa_chinensis	RchiOBHm_Chr2g0149341
rosa_laevigata	RLG00000020394
rosa_multiflora	Rmu_sc0000873.1_g000017
rosa_roxburghii	Rroxscaffold_2G00098200
rosa_rugosa	Rorug02G0410600 Rorug02G0410700 Rorug02G0410800
rosa_samantha	Rh2AG471400 Rh2BG483600 Rh2CG457300 Rh2DG492700
rosa_wichuraiana	Rw2G038360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1376, 1613
Acc36I	ACCTGC	2 cut(s)	1376, 1613
AccB7I	CCANNNNNTGG	1 cut(s)	1652
AccI	GTMKAC	1 cut(s)	1013
AccIII	TCCGGA	1 cut(s)	778
AciI	CCGC	2 cut(s)	71, 554
AcoI	YGGCCR	2 cut(s)	1450, 1811
AcsI	RAATTY	5 cut(s)	361, 754, 1634, 1955, 1991
AcuI	CTGAAG	3 cut(s)	501, 1025, 1188
AcyI	GRCGYC	1 cut(s)	2063
AfaI	GTAC	4 cut(s)	100, 699, 1137, 1316
AfiI	CCNNNNNNNGG	6 cut(s)	130, 199, 930, 1306, 1418, 1652
AflIII	ACRYGT	1 cut(s)	2305
AgeI	ACCGGT	1 cut(s)	682
AhdI	GACNNNNNGTC	1 cut(s)	2062
AjiI	CACGTC	1 cut(s)	1514
AjnI	CCWGG	3 cut(s)	1156, 1456, 2568
AjuI	GAANNNNNNNTTGG	4 cut(s)	706, 738, 1108, 1140
AloI	GAACNNNNNNTCC	2 cut(s)	619, 651
Alw21I	GWGCWC	1 cut(s)	190
Alw26I	GTCTC	6 cut(s)	20, 661, 776, 824, 1647, 1662
Aor13HI	TCCGGA	1 cut(s)	778
AoxI	GGCC	7 cut(s)	162, 368, 1450, 1460, 1582, 1811, 2615
ApeKI	GCWGC	2 cut(s)	104, 1310
ApoI	RAATTY	5 cut(s)	361, 754, 1634, 1955, 1991
AseI	ATTAAT	1 cut(s)	2102
AsiGI	ACCGGT	1 cut(s)	682
Asp700I	GAANNNNTTC	1 cut(s)	2367
AspA2I	CCTAGG	1 cut(s)	809
AspLEI	GCGC	1 cut(s)	1983
AspS9I	GGNCC	3 cut(s)	1054, 1460, 1562
AsuNHI	GCTAGC	1 cut(s)	728
AvaII	GGWCC	2 cut(s)	1054, 1562
AvrII	CCTAGG	1 cut(s)	809
BaeGI	GKGCMC	1 cut(s)	138
BaeI	ACNNNNGTAYC	2 cut(s)	861, 894
BamHI	GGATCC	2 cut(s)	440, 1099
BanII	GRGCYC	1 cut(s)	907
BarI	GAAGNNNNNNTAC	2 cut(s)	2024, 2056
BbsI	GAAGAC	3 cut(s)	621, 2061, 2282
Bbv12I	GWGCWC	1 cut(s)	190
BbvCI	CCTCAGC	1 cut(s)	159
BbvI	GCAGC	2 cut(s)	116, 1297
BccI	CCATC	8 cut(s)	58, 331, 390, 456, 640, 1150, 1483, 2294
BceAI	ACGGC	5 cut(s)	21, 117, 1219, 1465, 2433
BciT130I	CCWGG	3 cut(s)	1158, 1458, 2570
BciVI	GTATCC	1 cut(s)	862
BclI	TGATCA	1 cut(s)	2326
BcoDI	GTCTC	6 cut(s)	20, 661, 776, 824, 1647, 1662
BfaI	CTAG	6 cut(s)	498, 729, 810, 936, 1722, 2613
BfmI	CTRYAG	3 cut(s)	1383, 2044, 2373
BfuAI	ACCTGC	2 cut(s)	1376, 1613
BfuI	GTATCC	1 cut(s)	862
BglII	AGATCT	1 cut(s)	916
BisI	GCNGC	3 cut(s)	72, 105, 1311
BlnI	CCTAGG	1 cut(s)	809
BlsI	GCNGC	3 cut(s)	73, 106, 1312
Bme1390I	CCNGG	3 cut(s)	1158, 1458, 2570
Bme18I	GGWCC	2 cut(s)	1054, 1562
BmeRI	GACNNNNNGTC	1 cut(s)	2062
BmgBI	CACGTC	1 cut(s)	1514
BmgT120I	GGNCC	3 cut(s)	1054, 1460, 1562
BmiI	GGNNCC	7 cut(s)	208, 227, 394, 442, 904, 1101, 1330
BmrFI	CCNGG	3 cut(s)	1158, 1458, 2570
BmrI	ACTGGG	2 cut(s)	882, 939
BmsI	GCATC	5 cut(s)	58, 484, 956, 1339, 1438
BmtI	GCTAGC	1 cut(s)	732
BmuI	ACTGGG	2 cut(s)	882, 939
BpiI	GAAGAC	3 cut(s)	621, 2061, 2282
BplI	GAGNNNNNCTC	2 cut(s)	962, 994
BpmI	CTGGAG	3 cut(s)	232, 1454, 1767
Bpu10I	CCTNAGC	3 cut(s)	159, 1436, 2160
BpuEI	CTTGAG	1 cut(s)	441
Bsa29I	ATCGAT	1 cut(s)	62
BsaBI	GATNNNNATC	1 cut(s)	1428
BsaHI	GRCGYC	1 cut(s)	2063
BsaI	GGTCTC	1 cut(s)	1662
BsaWI	WCCGGW	2 cut(s)	682, 778
BsaXI	ACNNNNNCTCC	4 cut(s)	619, 649, 790, 820
Bsc4I	CCNNNNNNNGG	6 cut(s)	130, 199, 930, 1306, 1418, 1652
Bse118I	RCCGGY	1 cut(s)	682
Bse1I	ACTGG	7 cut(s)	249, 877, 934, 1239, 1784, 1941, 2548
Bse3DI	GCAATG	3 cut(s)	555, 1145, 1983
Bse8I	GATNNNNATC	1 cut(s)	1428
BseAI	TCCGGA	1 cut(s)	778
BseBI	CCWGG	3 cut(s)	1158, 1458, 2570
BseCI	ATCGAT	1 cut(s)	62
BseGI	GGATG	7 cut(s)	73, 382, 503, 820, 1528, 2434, 2515
BseJI	GATNNNNATC	1 cut(s)	1428
BseLI	CCNNNNNNNGG	6 cut(s)	130, 199, 930, 1306, 1418, 1652
BseMI	GCAATG	3 cut(s)	555, 1145, 1983
BseMII	CTCAG	5 cut(s)	150, 294, 2151, 2203, 2555
BseNI	ACTGG	7 cut(s)	249, 877, 934, 1239, 1784, 1941, 2548
BseRI	GAGGAG	3 cut(s)	363, 497, 2424
BseSI	GKGCMC	1 cut(s)	138
BseXI	GCAGC	2 cut(s)	116, 1297
BsgI	GTGCAG	1 cut(s)	1236
BshFI	GGCC	7 cut(s)	164, 370, 1452, 1462, 1584, 1813, 2617
BshTI	ACCGGT	1 cut(s)	682
BshVI	ATCGAT	1 cut(s)	62
BsiHKAI	GWGCWC	1 cut(s)	190
BsiSI	CCGG	2 cut(s)	683, 779
BslFI	GGGAC	4 cut(s)	1000, 1395, 1500, 2167
BslI	CCNNNNNNNGG	6 cut(s)	130, 199, 930, 1306, 1418, 1652
BsmAI	GTCTC	6 cut(s)	20, 661, 776, 824, 1647, 1662
BsmFI	GGGAC	4 cut(s)	1000, 1395, 1500, 2167
BsmI	GAATGC	4 cut(s)	55, 1665, 2367, 2369
BsnI	GGCC	7 cut(s)	164, 370, 1452, 1462, 1584, 1813, 2617
Bso31I	GGTCTC	1 cut(s)	1662
Bsp1286I	GDGCHC	3 cut(s)	138, 190, 907
Bsp13I	TCCGGA	1 cut(s)	778
Bsp1407I	TGTACA	1 cut(s)	697
Bsp19I	CCATGG	3 cut(s)	1105, 1278, 1804
BspACI	CCGC	2 cut(s)	71, 554
BspANI	GGCC	7 cut(s)	164, 370, 1452, 1462, 1584, 1813, 2617
BspCNI	CTCAG	5 cut(s)	151, 295, 2152, 2202, 2554
BspDI	ATCGAT	1 cut(s)	62
BspEI	TCCGGA	1 cut(s)	778
BspHI	TCATGA	1 cut(s)	18
BspLI	GGNNCC	7 cut(s)	208, 227, 394, 442, 904, 1101, 1330
BspMAI	CTGCAG	2 cut(s)	1387, 2048
BspMI	ACCTGC	2 cut(s)	1376, 1613
BspOI	GCTAGC	1 cut(s)	732
BspTNI	GGTCTC	1 cut(s)	1662
BsrDI	GCAATG	3 cut(s)	555, 1145, 1983
BsrFI	RCCGGY	1 cut(s)	682
BsrGI	TGTACA	1 cut(s)	697
BsrI	ACTGG	7 cut(s)	249, 877, 934, 1239, 1784, 1941, 2548
BssAI	RCCGGY	1 cut(s)	682
BssNI	GRCGYC	1 cut(s)	2063
BssT1I	CCWWGG	8 cut(s)	809, 907, 1105, 1278, 1518, 1565, 1804, 1834
Bst2UI	CCWGG	3 cut(s)	1158, 1458, 2570
Bst4CI	ACNGT	7 cut(s)	28, 286, 312, 431, 747, 1535, 1776
Bst6I	CTCTTC	2 cut(s)	808, 1129
BstACI	GRCGYC	1 cut(s)	2063
BstAUI	TGTACA	1 cut(s)	697
BstC8I	GCNNGC	6 cut(s)	513, 580, 730, 1454, 1586, 2178
BstDEI	CTNAG	7 cut(s)	159, 303, 1436, 1493, 2160, 2189, 2541
BstDSI	CCRYGG	3 cut(s)	1105, 1278, 1804
BstEII	GGTNACC	2 cut(s)	1026, 1625
BstENI	CCTNNNNNAGG	1 cut(s)	128
BstF5I	GGATG	7 cut(s)	73, 382, 503, 820, 1528, 2434, 2515
BstHHI	GCGC	1 cut(s)	1983
BstMAI	GTCTC	6 cut(s)	20, 661, 776, 824, 1647, 1662
BstMWI	GCNNNNNNNGC	4 cut(s)	725, 1147, 1187, 1731
BstNI	CCWGG	3 cut(s)	1158, 1458, 2570
BstNSI	RCATGY	2 cut(s)	2093, 2309
BstPI	GGTNACC	2 cut(s)	1026, 1625
BstSCI	CCNGG	3 cut(s)	1156, 1456, 2568
BstSFI	CTRYAG	3 cut(s)	1383, 2044, 2373
BstSLI	GKGCMC	1 cut(s)	138
BstV1I	GCAGC	2 cut(s)	116, 1297
BstV2I	GAAGAC	3 cut(s)	621, 2061, 2282
BstX2I	RGATCY	5 cut(s)	83, 440, 916, 1099, 2522
BstXI	CCANNNNNNTGG	2 cut(s)	389, 1325
BstYI	RGATCY	5 cut(s)	83, 440, 916, 1099, 2522
Bsu15I	ATCGAT	1 cut(s)	62
BsuI	GTATCC	1 cut(s)	862
BsuRI	GGCC	7 cut(s)	164, 370, 1452, 1462, 1584, 1813, 2617
BsuTUI	ATCGAT	1 cut(s)	62
BtgI	CCRYGG	3 cut(s)	1105, 1278, 1804
BtrI	CACGTC	1 cut(s)	1514
BtsCI	GGATG	7 cut(s)	73, 382, 503, 820, 1528, 2434, 2515
BtsI	GCAGTG	1 cut(s)	2053
BtsIMutI	CAGTG	7 cut(s)	89, 436, 1540, 2002, 2053, 2197, 2504
BveI	ACCTGC	2 cut(s)	1376, 1613
Cac8I	GCNNGC	6 cut(s)	513, 580, 730, 1454, 1586, 2178
CciI	TCATGA	1 cut(s)	18
CfoI	GCGC	1 cut(s)	1983
Cfr10I	RCCGGY	1 cut(s)	682
Cfr13I	GGNCC	3 cut(s)	1054, 1460, 1562
ClaI	ATCGAT	1 cut(s)	62
CseI	GACGC	1 cut(s)	2052
Csp6I	GTAC	4 cut(s)	99, 698, 1136, 1315
CspAI	ACCGGT	1 cut(s)	682
CspCI	CAANNNNNGTGG	4 cut(s)	1500, 1535, 1681, 1716
CviQI	GTAC	4 cut(s)	99, 698, 1136, 1315
DdeI	CTNAG	7 cut(s)	159, 303, 1436, 1493, 2160, 2189, 2541
DriI	GACNNNNNGTC	1 cut(s)	2062
EaeI	YGGCCR	2 cut(s)	1450, 1811
Eam1104I	CTCTTC	2 cut(s)	808, 1129
Eam1105I	GACNNNNNGTC	1 cut(s)	2062
EarI	CTCTTC	2 cut(s)	808, 1129
Eco130I	CCWWGG	8 cut(s)	809, 907, 1105, 1278, 1518, 1565, 1804, 1834
Eco147I	AGGCCT	1 cut(s)	370
Eco24I	GRGCYC	1 cut(s)	907
Eco31I	GGTCTC	1 cut(s)	1662
Eco47I	GGWCC	2 cut(s)	1054, 1562
Eco57I	CTGAAG	3 cut(s)	501, 1025, 1188
Eco91I	GGTNACC	2 cut(s)	1026, 1625
EcoNI	CCTNNNNNAGG	1 cut(s)	128
EcoO65I	GGTNACC	2 cut(s)	1026, 1625
EcoRI	GAATTC	1 cut(s)	1991
EcoRII	CCWGG	3 cut(s)	1156, 1456, 2568
EcoT14I	CCWWGG	8 cut(s)	809, 907, 1105, 1278, 1518, 1565, 1804, 1834
EcoT22I	ATGCAT	1 cut(s)	2369
EcoT38I	GRGCYC	1 cut(s)	907
ErhI	CCWWGG	8 cut(s)	809, 907, 1105, 1278, 1518, 1565, 1804, 1834
FalI	AAGNNNNNCTT	4 cut(s)	450, 482, 2067, 2099
FaqI	GGGAC	4 cut(s)	1000, 1395, 1500, 2167
FauNDI	CATATG	3 cut(s)	79, 1618, 2440
FbaI	TGATCA	1 cut(s)	2326
FblI	GTMKAC	1 cut(s)	1013
Fnu4HI	GCNGC	3 cut(s)	72, 105, 1311
FokI	GGATG	7 cut(s)	80, 369, 490, 807, 1535, 2421, 2522
FriOI	GRGCYC	1 cut(s)	907
Fsp4HI	GCNGC	3 cut(s)	72, 105, 1311
FspBI	CTAG	6 cut(s)	498, 729, 810, 936, 1722, 2613
GlaI	GCGC	1 cut(s)	1982
GluI	GCNGC	3 cut(s)	72, 105, 1311
GsuI	CTGGAG	3 cut(s)	232, 1454, 1767
HaeIII	GGCC	7 cut(s)	164, 370, 1452, 1462, 1584, 1813, 2617
HapII	CCGG	2 cut(s)	683, 779
HgaI	GACGC	1 cut(s)	2052
HhaI	GCGC	1 cut(s)	1983
Hin1I	GRCGYC	1 cut(s)	2063
Hin6I	GCGC	1 cut(s)	1981
HinP1I	GCGC	1 cut(s)	1981
HincII	GTYRAC	2 cut(s)	239, 2269
HindII	GTYRAC	2 cut(s)	239, 2269
HindIII	AAGCTT	4 cut(s)	513, 1366, 1885, 2033
HpaII	CCGG	2 cut(s)	683, 779
Hpy166II	GTNNAC	5 cut(s)	239, 1014, 1856, 2195, 2269
Hpy188I	TCNGA	9 cut(s)	147, 304, 319, 520, 1969, 2408, 2482, 2544, 2647
Hpy8I	GTNNAC	5 cut(s)	239, 1014, 1856, 2195, 2269
HpyAV	CCTTC	4 cut(s)	454, 1412, 1728, 2239
HpyCH4III	ACNGT	7 cut(s)	28, 286, 312, 431, 747, 1535, 1776
HpyCH4IV	ACGT	1 cut(s)	1513
HpyF10VI	GCNNNNNNNGC	4 cut(s)	725, 1147, 1187, 1731
HpyF3I	CTNAG	7 cut(s)	159, 303, 1436, 1493, 2160, 2189, 2541
HpySE526I	ACGT	1 cut(s)	1513
Hsp92I	GRCGYC	1 cut(s)	2063
HspAI	GCGC	1 cut(s)	1981
Kpn2I	TCCGGA	1 cut(s)	778
Ksp22I	TGATCA	1 cut(s)	2326
LmnI	GCTCC	6 cut(s)	193, 484, 902, 1473, 2173, 2470
Lsp1109I	GCAGC	2 cut(s)	116, 1297
LweI	GCATC	5 cut(s)	58, 484, 956, 1339, 1438
MaeI	CTAG	6 cut(s)	498, 729, 810, 936, 1722, 2613
MaeII	ACGT	1 cut(s)	1513
MaeIII	GTNAC	4 cut(s)	1026, 1609, 1625, 2563
MfeI	CAATTG	2 cut(s)	403, 894
MflI	RGATCY	5 cut(s)	83, 440, 916, 1099, 2522
MhlI	GDGCHC	3 cut(s)	138, 190, 907
MlyI	GAGTC	5 cut(s)	22, 1610, 1898, 2023, 2149
MmeI	TCCRAC	1 cut(s)	1670
Mph1103I	ATGCAT	1 cut(s)	2369
MroI	TCCGGA	1 cut(s)	778
MroXI	GAANNNNTTC	1 cut(s)	2367
MseI	TTAA	8 cut(s)	233, 359, 365, 957, 1370, 1890, 2102, 2210
MslI	CAYNNNNRTG	7 cut(s)	825, 849, 981, 1323, 1419, 1803, 2439
MspI	CCGG	2 cut(s)	683, 779
MspR9I	CCNGG	3 cut(s)	1158, 1458, 2570
MunI	CAATTG	2 cut(s)	403, 894
Mva1269I	GAATGC	4 cut(s)	55, 1665, 2367, 2369
MvaI	CCWGG	3 cut(s)	1158, 1458, 2570
MwoI	GCNNNNNNNGC	4 cut(s)	725, 1147, 1187, 1731
NcoI	CCATGG	3 cut(s)	1105, 1278, 1804
NdeI	CATATG	3 cut(s)	79, 1618, 2440
NheI	GCTAGC	1 cut(s)	728
NlaIV	GGNNCC	7 cut(s)	208, 227, 394, 442, 904, 1101, 1330
NmuCI	GTSAC	2 cut(s)	1625, 2563
NsiI	ATGCAT	1 cut(s)	2369
NspI	RCATGY	2 cut(s)	2093, 2309
PagI	TCATGA	1 cut(s)	18
PaqCI	CACCTGC	2 cut(s)	1376, 1613
PceI	AGGCCT	1 cut(s)	370
PciI	ACATGT	1 cut(s)	2305
PctI	GAATGC	4 cut(s)	55, 1665, 2367, 2369
PdmI	GAANNNNTTC	1 cut(s)	2367
PfeI	GAWTC	7 cut(s)	854, 1078, 1161, 1526, 1689, 1820, 2388
PflMI	CCANNNNNTGG	1 cut(s)	1652
PinAI	ACCGGT	1 cut(s)	682
PkrI	GCNGC	3 cut(s)	73, 106, 1312
PleI	GAGTC	5 cut(s)	21, 1609, 1898, 2022, 2149
PpsI	GAGTC	5 cut(s)	21, 1609, 1898, 2022, 2149
PscI	ACATGT	1 cut(s)	2305
PshBI	ATTAAT	1 cut(s)	2102
Psp6I	CCWGG	3 cut(s)	1156, 1456, 2568
PspEI	GGTNACC	2 cut(s)	1026, 1625
PspGI	CCWGG	3 cut(s)	1156, 1456, 2568
PspN4I	GGNNCC	7 cut(s)	208, 227, 394, 442, 904, 1101, 1330
PspPI	GGNCC	3 cut(s)	1054, 1460, 1562
PsrI	GAACNNNNNNTAC	2 cut(s)	190, 222
PstI	CTGCAG	2 cut(s)	1387, 2048
PsuI	RGATCY	5 cut(s)	83, 440, 916, 1099, 2522
RsaI	GTAC	4 cut(s)	100, 699, 1137, 1316
RsaNI	GTAC	4 cut(s)	99, 698, 1136, 1315
RseI	CAYNNNNRTG	7 cut(s)	825, 849, 981, 1323, 1419, 1803, 2439
SaqAI	TTAA	8 cut(s)	233, 359, 365, 957, 1370, 1890, 2102, 2210
SatI	GCNGC	3 cut(s)	72, 105, 1311
Sau96I	GGNCC	3 cut(s)	1054, 1460, 1562
SchI	GAGTC	5 cut(s)	22, 1610, 1898, 2023, 2149
ScrFI	CCNGG	3 cut(s)	1158, 1458, 2570
SduI	GDGCHC	3 cut(s)	138, 190, 907
SfaNI	GCATC	5 cut(s)	58, 484, 956, 1339, 1438
SfcI	CTRYAG	3 cut(s)	1383, 2044, 2373
SinI	GGWCC	2 cut(s)	1054, 1562
SmiMI	CAYNNNNRTG	7 cut(s)	825, 849, 981, 1323, 1419, 1803, 2439
SmlI	CTYRAG	1 cut(s)	456
SmoI	CTYRAG	1 cut(s)	456
SseBI	AGGCCT	1 cut(s)	370
SsiI	CCGC	2 cut(s)	71, 554
SspI	AATATT	2 cut(s)	862, 955
SspMI	CTAG	6 cut(s)	498, 729, 810, 936, 1722, 2613
StuI	AGGCCT	1 cut(s)	370
StyD4I	CCNGG	3 cut(s)	1156, 1456, 2568
StyI	CCWWGG	8 cut(s)	809, 907, 1105, 1278, 1518, 1565, 1804, 1834
TaaI	ACNGT	7 cut(s)	28, 286, 312, 431, 747, 1535, 1776
TaiI	ACGT	1 cut(s)	1516
TatI	WGTACW	1 cut(s)	697
TauI	GCSGC	1 cut(s)	74
TfiI	GAWTC	7 cut(s)	854, 1078, 1161, 1526, 1689, 1820, 2388
Tru1I	TTAA	8 cut(s)	233, 359, 365, 957, 1370, 1890, 2102, 2210
Tru9I	TTAA	8 cut(s)	233, 359, 365, 957, 1370, 1890, 2102, 2210
TscAI	CASTG	7 cut(s)	96, 436, 1540, 2002, 2053, 2197, 2504
TseFI	GTSAC	2 cut(s)	1625, 2563
TseI	GCWGC	2 cut(s)	104, 1310
Tsp45I	GTSAC	2 cut(s)	1625, 2563
TspGWI	ACGGA	2 cut(s)	23, 453
TspRI	CASTG	7 cut(s)	96, 436, 1540, 2002, 2053, 2197, 2504
Van91I	CCANNNNNTGG	1 cut(s)	1652
VpaK11BI	GGWCC	2 cut(s)	1054, 1562
VspI	ATTAAT	1 cut(s)	2102
XagI	CCTNNNNNAGG	1 cut(s)	128
XapI	RAATTY	5 cut(s)	361, 754, 1634, 1955, 1991
XbaI	TCTAGA	1 cut(s)	935
XceI	RCATGY	2 cut(s)	2093, 2309
XmaJI	CCTAGG	1 cut(s)	809
XmiI	GTMKAC	1 cut(s)	1013
XmnI	GAANNNNTTC	1 cut(s)	2367
XspI	CTAG	6 cut(s)	498, 729, 810, 936, 1722, 2613
Zsp2I	ATGCAT	1 cut(s)	2369

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.