Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0004599

Physical Location & Seq

Genomic Coordinates Forward (+)

6374146 .. 6379457

Gene Structure 5312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g08300.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3045 bp

ATGGCAACTGAGGGTCTCATGAGAATGGTAGAAAGCAGCAGGGGAAGGAAGTGGCCCCCATCTAAGGATGCTGCAACATTTGGATCTCCAGTGAGAAGTATGACAGCAGAGGAGTCGGGCTTCATTTCAAAGGGGCATGGCTTTAAGAGAGAGAGAGCTGAAGTTATACCCAGTCGAAGTGGTAGTGCTCCACCAAGTATGGAAGGTTCCTTTTCCTCTATTGAAAACCTATTAAGTCAACAGAACTCCAGCATGAAAACAAGCTTGACTAATTTAAACAACACTGTGGATAATGTTGAGAATGAAGAAAAACTGCGTTCAGACCCAGCTTATATGGCATACTACTTATCAAATATGAACTTGAATGCAAAGCTGCCTCCACCGCTTATTGTACGGGAGAATCATCATGTTGTCCGCCATAGTGGTGGTCTAGGAACCAAAGGGAGACTGACTTCTTTAGATGACAGCGCCAGGTCCTTGCATCTTTCTCAAGGTTCTCTCTCAACTCTTCAGGAGGACCCTAATGATGCTAGTTCATCCAGAAAGTCTAATGATAACTTGGCAGAAGATAGTGTTGCAGTAATGCCTGTCAAGGACACGGTTTCTTTGGCAAGTTATAATAAGAGTATAGTGGATCTCATACAGCAAGACTTCCCCCGGACTCCATCTCCTGTATATAATCACTCCCTCACATCAAGTCTTGGAACAACAGATGAACCAATTGATAGTGATGTACACTCACTTTCACCAAATGTTTCTTCTGTCAACAAGTCAAAATTACCTGAACCAAATTCAGACTCTATCAATGATTGTCCAGATACAAGTGCCTCGGACGCGCTTGCTCGTGGGTTCATACCAAATGATGTTCCTTTGCCTACCACCTCCCCAAGTGCTCAACATCATGATGTTACAGGGAACCTACAAAAAGATGAATCTAATATTGAGCATGATGGGTCGGGGAATAATGCATCGATTACTGGTGACCTTGGGTTGGATCTTTCCAGAGCAAGAGCAGCTAAAGTTGATACCAACAATAACAAGCAACAAGAACAATCTTATGGGAGGTATCTACCACAACACAATTTGTCTACACAACAAGGGGTTCCATATCAACTTCAGGGGTTCCAGGCACAATTAGTTTCACAAGGAATGAATCATTTGCAAAGCAGAATGCAAAATCTTCCCCACGGCTACCCAAAATTCTCTTCTATTGAGACACAACCATCATTGCTCTCACCTGGTGTCACACCTCCCTTATATGGTACAACCACAACATATATGACTTCAGGAAGTCCATTCTACTCAAATTATCAGTCGTCTAGTGTGTTTCCTACACAATATGGAATGGGTGGATATGCTTTAGGTTCTACTTATCTTCCTTCATATATGCCTGGGTATGCTTCTCATGGGTCTTTTCAAATGCTCTTTGATGCTACTTTGGGGCCAAGTTTCAATGGTCGAACTGCTGATGTTTCAAGAGGGGAGAGGATTCCCCATGAAGGTGATATGCAACACCCTAGTAGATCTTATGGGCAACATGGGCCAGTGCTCCAGCCACCTTTTGTGGATCCCCTTTATACGCAATACTATCCTCGTCTGTTGGAGGATTCATATGGTGCTTCAATTCCTCATGGTCACTTGGCGTCAAGGGGTGTTGTCAGAGGCCAACTTGCGCAACAAGAATTAAATGTTACTGCTTATAGGGCTGACCCAAAATTCCACACTTCAACTAATGGTAATCTGGGTATTCTAAGTCCAAGGAATGTGGGAAACAATGGTAGTGGTTATTATGGACATCCTTCTGGCATGGGTGTAGTGACACAGTTTCCAGCCTCACATCTCGGTAGTCCAATATTGCCATCATCTCCAATGGGCAGAATGAATCATCTTGGTCGGAAATATGAATTGAGGTCTCCTGAAGGTTCTGTTAGTGGAGTATATTCTGGGTGGCAAGGGCAGAGAAGCTCCATCTTGGATGATCCTAGAAGACACTCTGTACTTGAAGAACTGAAATCTAGTAATACCCGTAAATTTGAACTTTCTGATATAGCTGGGCGCATTGTTGATTTCAGTGTCGATCAACATGGGAGTCGATTTATTCAACAGAAGCTAGAGTACTGCAGTTCTGAAGACAAGGCATCTGTTTTCAAAGAGATTCTTCCACGTGCTTCAAAATTAATGACGGATGTATTTGGGAATTATGTTATTCAAAAGTTTTTTGAATATGGGAGTCCTGAGCAGAAAAAGGAGCTTGCAGATCAACTTGCTGGGCAAGTTTTGCCTTTAAGTTTGCAGATGTATGGTTGTCGTGTAATCCAGAAGGCTCTCGAAGTTATTGAGCTTGACCAGAAAATACAACTTGTGCATGAGCTTGATGGACATGTCATGACATGTGTACGAGATCAAAATGGAAATCATGTAATACAGAAGTGTATAGAATGCATTCCCACAGAGAAAATTGGATTTATCATTTCTGCTTTTCAAGGACAAGTTGCCTCCCTTTCTACTCATCCCTATGGCTGTCGTGTCATACAGAGAGTTTTGGAGCATTGTTCAGATGACCTTCAAAGTCAGTGTATAGTTGATGAGATCTTGGAATCTACTTATGATCTTGCTCAAGATCAGTATGGCAATTATGTTACCCAGCATGTTCTGGAAAGGGGAAAGCCCAACGAAAGAAGCCAAATTATCAGCAAGTTGGTTGGGAAGATTGTACGATTGAGTCAGCATAAATATGCATCAAATGTTGTTGAGAAGTGTCTGGAGTATGGTGATACTACTGAGCGCGAGCTCTTGATTGAGGAAATCATTGGACAAATGGAAGAAAATGATAGCCTGTTGCCAATGATGAAGGACCAATTTGCCAATTATGTGGTTCAGAAGGTTCTTGAAACAAGCAATGATAGACAGCGGGAAATACTGCTCAAACTTATAAGAGTTCATATTGATGCTCTGAAGAAATATACTTATGGGAAACACATAGTTGTTCGGTTTCAACAGCTATCCGGTGAAGATGTCCAAACCCCAGAAGCTGAGGGGGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NABP PUF

Protein Analysis

1015

Amino Acids

111.96

Weight (kDa)

5.99

Isoelectric Point (pI)

46.61

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20250 AT3G20250 AT3G20250
fragaria_vesca	FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910
malus_domestica	MD09G1165700.v1.1 MD17G1161900.v1.1
prunus_persica	Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1
pyrus_communis	pycom07g18900 pycom09g08300 pycom17g15500
rosa_chinensis	RchiOBHm_Chr2g0149341
rosa_laevigata	RLG00000020394
rosa_multiflora	Rmu_sc0000873.1_g000017
rosa_roxburghii	Rroxscaffold_2G00098200
rosa_rugosa	Rorug02G0410600 Rorug02G0410700 Rorug02G0410800
rosa_samantha	Rh2AG471400 Rh2BG483600 Rh2CG457300 Rh2DG492700
rosa_wichuraiana	Rw2G038360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	618, 2933
Acc16I	TGCGCA	1 cut(s)	1674
AccI	GTMKAC	1 cut(s)	1088
AccII	CGCG	2 cut(s)	836, 2787
AciI	CCGC	3 cut(s)	383, 415, 2911
AclWI	GGATC	6 cut(s)	91, 642, 1002, 1562, 1575, 1973
AcsI	RAATTY	4 cut(s)	790, 1199, 1715, 2030
AcuI	CTGAAG	7 cut(s)	180, 494, 1100, 1269, 1938, 2148, 2975
AcvI	CACGTG	1 cut(s)	2165
AcyI	GRCGYC	1 cut(s)	1643
AdeI	CACNNNGTG	1 cut(s)	1241
AfaI	GTAC	7 cut(s)	393, 735, 1264, 1998, 2117, 2397, 2715
AfiI	CCNNNNNNNGG	4 cut(s)	64, 991, 1259, 1499
AflIII	ACRYGT	2 cut(s)	2380, 2390
AjnI	CCWGG	4 cut(s)	470, 1125, 1237, 1390
AjuI	GAANNNNNNNTTGG	4 cut(s)	742, 774, 1189, 1221
Alw21I	GWGCWC	4 cut(s)	190, 895, 1551, 2793
Alw26I	GTCTC	4 cut(s)	20, 439, 1208, 1917
AlwI	GGATC	6 cut(s)	91, 642, 1002, 1562, 1575, 1973
AlwNI	CAGNNNCTG	1 cut(s)	3032
AoxI	GGCC	4 cut(s)	53, 1442, 1541, 1663
ApeKI	GCWGC	4 cut(s)	36, 71, 373, 1013
ApoI	RAATTY	4 cut(s)	790, 1199, 1715, 2030
ArsI	GACNNNNNNTTYG	2 cut(s)	1298, 1330
AseI	ATTAAT	1 cut(s)	2177
Asp700I	GAANNNNTTC	1 cut(s)	2442
AspLEI	GCGC	5 cut(s)	470, 838, 1675, 2058, 2787
AspS9I	GGNCC	6 cut(s)	54, 474, 517, 1442, 1541, 2854
AsuC2I	CCSGG	1 cut(s)	658
AsuHPI	GGTGA	6 cut(s)	738, 992, 1227, 1514, 2783, 3020
AvaII	GGWCC	3 cut(s)	474, 517, 2854
BamHI	GGATCC	1 cut(s)	1567
BanII	GRGCYC	1 cut(s)	2793
BarI	GAAGNNNNNNTAC	2 cut(s)	2099, 2131
BauI	CACGAG	1 cut(s)	843
BbrPI	CACGTG	1 cut(s)	2165
BbsI	GAAGAC	2 cut(s)	1993, 2136
Bbv12I	GWGCWC	4 cut(s)	190, 895, 1551, 2793
BbvCI	CCTCAGC	1 cut(s)	3033
BbvI	GCAGC	4 cut(s)	48, 58, 360, 1025
BccI	CCATC	7 cut(s)	67, 673, 944, 1231, 1867, 1976, 2369
BceAI	ACGGC	1 cut(s)	1204
BcgI	CGANNNNNNTGC	2 cut(s)	96, 130
BciT130I	CCWGG	4 cut(s)	472, 1127, 1239, 1392
BcnI	CCSGG	1 cut(s)	658
BcoDI	GTCTC	4 cut(s)	20, 439, 1208, 1917
BfaI	CTAG	7 cut(s)	431, 531, 1320, 1518, 1983, 2016, 2111
BfmI	CTRYAG	1 cut(s)	2119
BfoI	RGCGCY	1 cut(s)	471
BglII	AGATCT	2 cut(s)	1523, 2589
BisI	GCNGC	4 cut(s)	37, 72, 374, 1014
BlsI	GCNGC	4 cut(s)	38, 73, 375, 1015
BmcAI	AGTACT	1 cut(s)	2117
Bme1390I	CCNGG	5 cut(s)	472, 658, 1127, 1239, 1392
Bme18I	GGWCC	3 cut(s)	474, 517, 2854
BmgT120I	GGNCC	6 cut(s)	54, 474, 517, 1442, 1541, 2854
BmiI	GGNNCC	9 cut(s)	56, 208, 436, 519, 917, 1104, 1124, 1443, 1569
BmrFI	CCNGG	5 cut(s)	472, 658, 1127, 1239, 1392
BmrI	ACTGGG	1 cut(s)	165
BmsI	GCATC	8 cut(s)	58, 490, 517, 977, 1420, 2147, 2747, 2938
BmuI	ACTGGG	1 cut(s)	165
BpiI	GAAGAC	2 cut(s)	1993, 2136
BpmI	CTGGAG	4 cut(s)	72, 232, 1535, 2783
Bpu10I	CCTNAGC	2 cut(s)	2235, 3033
BpuEI	CTTGAG	2 cut(s)	474, 2601
BpuMI	CCSGG	1 cut(s)	658
Bsa29I	ATCGAT	1 cut(s)	971
BsaAI	YACGTR	1 cut(s)	2165
BsaHI	GRCGYC	1 cut(s)	1643
BsaI	GGTCTC	2 cut(s)	20, 1917
BsaJI	CCNNGG	6 cut(s)	656, 828, 985, 1186, 1391, 1757
BsaWI	WCCGGW	1 cut(s)	3005
BsaXI	ACNNNNNCTCC	2 cut(s)	652, 682
Bsc4I	CCNNNNNNNGG	4 cut(s)	64, 991, 1259, 1499
Bse1I	ACTGG	4 cut(s)	89, 171, 982, 1544
Bse3DI	GCAATG	2 cut(s)	1226, 2905
BseBI	CCWGG	4 cut(s)	472, 1127, 1239, 1392
BseCI	ATCGAT	1 cut(s)	971
BseDI	CCNNGG	6 cut(s)	656, 828, 985, 1186, 1391, 1757
BseGI	GGATG	6 cut(s)	73, 536, 1795, 1981, 2191, 2509
BseLI	CCNNNNNNNGG	4 cut(s)	64, 991, 1259, 1499
BseMI	GCAATG	2 cut(s)	1226, 2905
BseMII	CTCAG	3 cut(s)	2226, 2772, 3024
BseNI	ACTGG	4 cut(s)	89, 171, 982, 1544
BseRI	GAGGAG	1 cut(s)	125
BseXI	GCAGC	4 cut(s)	48, 58, 360, 1025
BseYI	CCCAGC	4 cut(s)	325, 2051, 2267, 2643
Bsh1236I	CGCG	2 cut(s)	836, 2787
BshFI	GGCC	4 cut(s)	55, 1444, 1543, 1665
BshVI	ATCGAT	1 cut(s)	971
BsiHKAI	GWGCWC	4 cut(s)	190, 895, 1551, 2793
BsiSI	CCGG	2 cut(s)	658, 3006
BslI	CCNNNNNNNGG	4 cut(s)	64, 991, 1259, 1499
BsmAI	GTCTC	4 cut(s)	20, 439, 1208, 1917
BsmI	GAATGC	4 cut(s)	370, 1176, 2442, 2444
BsnI	GGCC	4 cut(s)	55, 1444, 1543, 1665
Bso31I	GGTCTC	2 cut(s)	20, 1917
Bsp1286I	GDGCHC	4 cut(s)	190, 895, 1551, 2793
Bsp1407I	TGTACA	1 cut(s)	733
BspACI	CCGC	3 cut(s)	383, 415, 2911
BspANI	GGCC	4 cut(s)	55, 1444, 1543, 1665
BspCNI	CTCAG	3 cut(s)	2227, 2773, 3025
BspDI	ATCGAT	1 cut(s)	971
BspFNI	CGCG	2 cut(s)	836, 2787
BspHI	TCATGA	3 cut(s)	18, 901, 2385
BspLI	GGNNCC	9 cut(s)	56, 208, 436, 519, 917, 1104, 1124, 1443, 1569
BspMAI	CTGCAG	1 cut(s)	2123
BspPI	GGATC	6 cut(s)	91, 642, 1002, 1562, 1575, 1973
BspTNI	GGTCTC	2 cut(s)	20, 1917
BsrDI	GCAATG	2 cut(s)	1226, 2905
BsrGI	TGTACA	1 cut(s)	733
BsrI	ACTGG	4 cut(s)	89, 171, 982, 1544
BssECI	CCNNGG	6 cut(s)	656, 828, 985, 1186, 1391, 1757
BssNI	GRCGYC	1 cut(s)	1643
BssSI	CACGAG	1 cut(s)	843
BssT1I	CCWWGG	2 cut(s)	985, 1757
Bst2BI	CACGAG	1 cut(s)	843
Bst2UI	CCWGG	4 cut(s)	472, 1127, 1239, 1392
Bst4CI	ACNGT	3 cut(s)	286, 601, 1824
Bst6I	CTCTTC	2 cut(s)	513, 1210
BstACI	GRCGYC	1 cut(s)	1643
BstAPI	GCANNNNNTGC	1 cut(s)	2278
BstAUI	TGTACA	1 cut(s)	733
BstBAI	YACGTR	1 cut(s)	2165
BstC8I	GCNNGC	3 cut(s)	840, 2253, 2789
BstDEI	CTNAG	6 cut(s)	9, 63, 1751, 2235, 2781, 3033
BstDSI	CCRYGG	1 cut(s)	1186
BstEII	GGTNACC	1 cut(s)	980
BstF5I	GGATG	6 cut(s)	73, 536, 1795, 1981, 2191, 2509
BstFNI	CGCG	2 cut(s)	836, 2787
BstH2I	RGCGCY	1 cut(s)	471
BstHHI	GCGC	5 cut(s)	470, 838, 1675, 2058, 2787
BstMAI	GTCTC	4 cut(s)	20, 439, 1208, 1917
BstMWI	GCNNNNNNNGC	7 cut(s)	335, 382, 833, 1013, 1540, 1703, 2278
BstNI	CCWGG	4 cut(s)	472, 1127, 1239, 1392
BstNSI	RCATGY	3 cut(s)	2384, 2394, 2651
BstPI	GGTNACC	1 cut(s)	980
BstSCI	CCNGG	5 cut(s)	470, 656, 1125, 1237, 1390
BstSFI	CTRYAG	1 cut(s)	2119
BstUI	CGCG	2 cut(s)	836, 2787
BstV1I	GCAGC	4 cut(s)	48, 58, 360, 1025
BstV2I	GAAGAC	2 cut(s)	1993, 2136
BstX2I	RGATCY	6 cut(s)	83, 634, 994, 1523, 1567, 2589
BstXI	CCANNNNNNTGG	2 cut(s)	425, 2872
BstYI	RGATCY	6 cut(s)	83, 634, 994, 1523, 1567, 2589
Bsu15I	ATCGAT	1 cut(s)	971
BsuRI	GGCC	4 cut(s)	55, 1444, 1543, 1665
BsuTUI	ATCGAT	1 cut(s)	971
BtgI	CCRYGG	1 cut(s)	1186
BtsCI	GGATG	6 cut(s)	73, 536, 1795, 1981, 2191, 2509
BtsIMutI	CAGTG	5 cut(s)	96, 282, 1551, 2077, 2579
Cac8I	GCNNGC	3 cut(s)	840, 2253, 2789
CaiI	CAGNNNCTG	1 cut(s)	3032
CciI	TCATGA	3 cut(s)	18, 901, 2385
CfoI	GCGC	5 cut(s)	470, 838, 1675, 2058, 2787
Cfr13I	GGNCC	6 cut(s)	54, 474, 517, 1442, 1541, 2854
ClaI	ATCGAT	1 cut(s)	971
CseI	GACGC	2 cut(s)	842, 1632
CsiI	ACCWGGT	1 cut(s)	1237
Csp6I	GTAC	7 cut(s)	392, 734, 1263, 1997, 2116, 2396, 2714
CspCI	CAANNNNNGTGG	4 cut(s)	1746, 1762, 1781, 1797
CviQI	GTAC	7 cut(s)	392, 734, 1263, 1997, 2116, 2396, 2714
DdeI	CTNAG	6 cut(s)	9, 63, 1751, 2235, 2781, 3033
DraI	TTTAAA	1 cut(s)	276
DraIII	CACNNNGTG	1 cut(s)	1241
Eam1104I	CTCTTC	2 cut(s)	513, 1210
EarI	CTCTTC	2 cut(s)	513, 1210
EciI	GGCGGA	1 cut(s)	404
Ecl136II	GAGCTC	1 cut(s)	2791
Eco130I	CCWWGG	2 cut(s)	985, 1757
Eco24I	GRGCYC	1 cut(s)	2793
Eco31I	GGTCTC	2 cut(s)	20, 1917
Eco47I	GGWCC	3 cut(s)	474, 517, 2854
Eco53kI	GAGCTC	1 cut(s)	2791
Eco57I	CTGAAG	7 cut(s)	180, 494, 1100, 1269, 1938, 2148, 2975
Eco72I	CACGTG	1 cut(s)	2165
Eco91I	GGTNACC	1 cut(s)	980
EcoICRI	GAGCTC	1 cut(s)	2791
EcoO109I	RGGNCCY	2 cut(s)	474, 517
EcoO65I	GGTNACC	1 cut(s)	980
EcoRII	CCWGG	4 cut(s)	470, 1125, 1237, 1390
EcoT14I	CCWWGG	2 cut(s)	985, 1757
EcoT22I	ATGCAT	3 cut(s)	970, 2444, 2740
EcoT38I	GRGCYC	1 cut(s)	2793
ErhI	CCWWGG	2 cut(s)	985, 1757
FalI	AAGNNNNNCTT	2 cut(s)	2142, 2174
FauI	CCCGC	1 cut(s)	2904
FauNDI	CATATG	1 cut(s)	1612
FblI	GTMKAC	1 cut(s)	1088
Fnu4HI	GCNGC	4 cut(s)	37, 72, 374, 1014
FokI	GGATG	6 cut(s)	80, 523, 1782, 1988, 2198, 2496
FriOI	GRGCYC	1 cut(s)	2793
Fsp4HI	GCNGC	4 cut(s)	37, 72, 374, 1014
FspBI	CTAG	7 cut(s)	431, 531, 1320, 1518, 1983, 2016, 2111
FspI	TGCGCA	1 cut(s)	1674
GlaI	GCGC	5 cut(s)	469, 837, 1674, 2057, 2786
GluI	GCNGC	4 cut(s)	37, 72, 374, 1014
GsaI	CCCAGC	4 cut(s)	329, 2055, 2271, 2647
GsuI	CTGGAG	4 cut(s)	72, 232, 1535, 2783
HaeII	RGCGCY	1 cut(s)	471
HaeIII	GGCC	4 cut(s)	55, 1444, 1543, 1665
HapII	CCGG	2 cut(s)	658, 3006
HgaI	GACGC	2 cut(s)	842, 1632
HhaI	GCGC	5 cut(s)	470, 838, 1675, 2058, 2787
Hin1I	GRCGYC	1 cut(s)	1643
Hin6I	GCGC	5 cut(s)	468, 836, 1673, 2056, 2785
HinP1I	GCGC	5 cut(s)	468, 836, 1673, 2056, 2785
HincII	GTYRAC	2 cut(s)	239, 766
HindII	GTYRAC	2 cut(s)	239, 766
HindIII	AAGCTT	1 cut(s)	262
HpaII	CCGG	2 cut(s)	658, 3006
HphI	GGTGA	6 cut(s)	738, 992, 1227, 1514, 2783, 3020
Hpy166II	GTNNAC	5 cut(s)	239, 736, 766, 1089, 2396
Hpy8I	GTNNAC	5 cut(s)	239, 736, 766, 1089, 2396
HpyCH4III	ACNGT	3 cut(s)	286, 601, 1824
HpyCH4IV	ACGT	1 cut(s)	2164
HpyF10VI	GCNNNNNNNGC	7 cut(s)	335, 382, 833, 1013, 1540, 1703, 2278
HpyF3I	CTNAG	6 cut(s)	9, 63, 1751, 2235, 2781, 3033
HpySE526I	ACGT	1 cut(s)	2164
Hsp92I	GRCGYC	1 cut(s)	1643
HspAI	GCGC	5 cut(s)	468, 836, 1673, 2056, 2785
LmnI	GCTCC	5 cut(s)	193, 1554, 1970, 2248, 2545
Lsp1109I	GCAGC	4 cut(s)	48, 58, 360, 1025
LweI	GCATC	8 cut(s)	58, 490, 517, 977, 1420, 2147, 2747, 2938
MabI	ACCWGGT	1 cut(s)	1237
MaeI	CTAG	7 cut(s)	431, 531, 1320, 1518, 1983, 2016, 2111
MaeII	ACGT	1 cut(s)	2164
MaeIII	GTNAC	7 cut(s)	907, 980, 1243, 1634, 1690, 1816, 2638
MfeI	CAATTG	1 cut(s)	720
MflI	RGATCY	6 cut(s)	83, 634, 994, 1523, 1567, 2589
MhlI	GDGCHC	4 cut(s)	190, 895, 1551, 2793
MlyI	GAGTC	6 cut(s)	122, 655, 791, 2098, 2239, 2731
MmeI	TCCRAC	3 cut(s)	972, 1581, 1874
Mph1103I	ATGCAT	3 cut(s)	970, 2444, 2740
MroXI	GAANNNNTTC	1 cut(s)	2442
MseI	TTAA	6 cut(s)	144, 233, 275, 1685, 2177, 2285
MslI	CAYNNNNRTG	7 cut(s)	23, 423, 903, 1500, 2514, 2733, 2946
MspA1I	CMGCKG	1 cut(s)	2911
MspI	CCGG	2 cut(s)	658, 3006
MspR9I	CCNGG	5 cut(s)	472, 658, 1127, 1239, 1392
MunI	CAATTG	1 cut(s)	720
Mva1269I	GAATGC	4 cut(s)	370, 1176, 2442, 2444
MvaI	CCWGG	4 cut(s)	472, 1127, 1239, 1392
MvnI	CGCG	2 cut(s)	836, 2787
MwoI	GCNNNNNNNGC	7 cut(s)	335, 382, 833, 1013, 1540, 1703, 2278
NciI	CCSGG	1 cut(s)	658
NdeI	CATATG	1 cut(s)	1612
NlaIV	GGNNCC	9 cut(s)	56, 208, 436, 519, 917, 1104, 1124, 1443, 1569
NmuCI	GTSAC	4 cut(s)	980, 1243, 1634, 1816
NsbI	TGCGCA	1 cut(s)	1674
NsiI	ATGCAT	3 cut(s)	970, 2444, 2740
NspI	RCATGY	3 cut(s)	2384, 2394, 2651
PagI	TCATGA	3 cut(s)	18, 901, 2385
PciI	ACATGT	2 cut(s)	2380, 2390
PctI	GAATGC	4 cut(s)	370, 1176, 2442, 2444
PdmI	GAANNNNTTC	1 cut(s)	2442
PfeI	GAWTC	8 cut(s)	400, 932, 1153, 1489, 1607, 1882, 2155, 2597
PkrI	GCNGC	4 cut(s)	38, 73, 375, 1015
PleI	GAGTC	6 cut(s)	121, 655, 791, 2097, 2238, 2730
PmaCI	CACGTG	1 cut(s)	2165
PmlI	CACGTG	1 cut(s)	2165
PpsI	GAGTC	6 cut(s)	121, 655, 791, 2097, 2238, 2730
Ppu21I	YACGTR	1 cut(s)	2165
PpuMI	RGGWCCY	2 cut(s)	474, 517
PscI	ACATGT	2 cut(s)	2380, 2390
PshBI	ATTAAT	1 cut(s)	2177
PsiI	TTATAA	2 cut(s)	618, 2933
Psp124BI	GAGCTC	1 cut(s)	2793
Psp5II	RGGWCCY	2 cut(s)	474, 517
Psp6I	CCWGG	4 cut(s)	470, 1125, 1237, 1390
PspCI	CACGTG	1 cut(s)	2165
PspEI	GGTNACC	1 cut(s)	980
PspFI	CCCAGC	4 cut(s)	325, 2051, 2267, 2643
PspGI	CCWGG	4 cut(s)	470, 1125, 1237, 1390
PspN4I	GGNNCC	9 cut(s)	56, 208, 436, 519, 917, 1104, 1124, 1443, 1569
PspPI	GGNCC	6 cut(s)	54, 474, 517, 1442, 1541, 2854
PspPPI	RGGWCCY	2 cut(s)	474, 517
PsrI	GAACNNNNNNTAC	2 cut(s)	190, 222
PstI	CTGCAG	1 cut(s)	2123
PstNI	CAGNNNCTG	1 cut(s)	3032
PsuI	RGATCY	6 cut(s)	83, 634, 994, 1523, 1567, 2589
RsaI	GTAC	7 cut(s)	393, 735, 1264, 1998, 2117, 2397, 2715
RsaNI	GTAC	7 cut(s)	392, 734, 1263, 1997, 2116, 2396, 2714
RseI	CAYNNNNRTG	7 cut(s)	23, 423, 903, 1500, 2514, 2733, 2946
SacI	GAGCTC	1 cut(s)	2793
SaqAI	TTAA	6 cut(s)	144, 233, 275, 1685, 2177, 2285
SatI	GCNGC	4 cut(s)	37, 72, 374, 1014
Sau96I	GGNCC	6 cut(s)	54, 474, 517, 1442, 1541, 2854
ScaI	AGTACT	1 cut(s)	2117
SchI	GAGTC	6 cut(s)	122, 655, 791, 2098, 2239, 2731
ScrFI	CCNGG	5 cut(s)	472, 658, 1127, 1239, 1392
SduI	GDGCHC	4 cut(s)	190, 895, 1551, 2793
SexAI	ACCWGGT	1 cut(s)	1237
SfaNI	GCATC	8 cut(s)	58, 490, 517, 977, 1420, 2147, 2747, 2938
SfcI	CTRYAG	1 cut(s)	2119
SinI	GGWCC	3 cut(s)	474, 517, 2854
SmiMI	CAYNNNNRTG	7 cut(s)	23, 423, 903, 1500, 2514, 2733, 2946
SmlI	CTYRAG	2 cut(s)	489, 2616
SmoI	CTYRAG	2 cut(s)	489, 2616
SsiI	CCGC	3 cut(s)	383, 415, 2911
SspI	AATATT	2 cut(s)	940, 1854
SspMI	CTAG	7 cut(s)	431, 531, 1320, 1518, 1983, 2016, 2111
SstI	GAGCTC	1 cut(s)	2793
StyD4I	CCNGG	5 cut(s)	470, 656, 1125, 1237, 1390
StyI	CCWWGG	2 cut(s)	985, 1757
TaaI	ACNGT	3 cut(s)	286, 601, 1824
TaiI	ACGT	1 cut(s)	2167
TaqI	TCGA	6 cut(s)	175, 971, 1459, 2076, 2092, 2328
TatI	WGTACW	3 cut(s)	733, 1996, 2115
TfiI	GAWTC	8 cut(s)	400, 932, 1153, 1489, 1607, 1882, 2155, 2597
Tru1I	TTAA	6 cut(s)	144, 233, 275, 1685, 2177, 2285
Tru9I	TTAA	6 cut(s)	144, 233, 275, 1685, 2177, 2285
TscAI	CASTG	5 cut(s)	96, 289, 1551, 2077, 2579
TseFI	GTSAC	4 cut(s)	980, 1243, 1634, 1816
TseI	GCWGC	4 cut(s)	36, 71, 373, 1013
Tsp45I	GTSAC	4 cut(s)	980, 1243, 1634, 1816
TspGWI	ACGGA	1 cut(s)	2198
TspRI	CASTG	5 cut(s)	96, 289, 1551, 2077, 2579
VpaK11BI	GGWCC	3 cut(s)	474, 517, 2854
VspI	ATTAAT	1 cut(s)	2177
XapI	RAATTY	4 cut(s)	790, 1199, 1715, 2030
XceI	RCATGY	3 cut(s)	2384, 2394, 2651
XmiI	GTMKAC	1 cut(s)	1088
XmnI	GAANNNNTTC	1 cut(s)	2442
XspI	CTAG	7 cut(s)	431, 531, 1320, 1518, 1983, 2016, 2111
ZrmI	AGTACT	1 cut(s)	2117
Zsp2I	ATGCAT	3 cut(s)	970, 2444, 2740

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.