Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0004599

Physical Location & Seq

Genomic Coordinates Reverse (-)

15954957 .. 15967847

Gene Structure 12891 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1161900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3063 bp

ATGCATTATTCTGGTAGGATGGCAACCGAGGGTCTCATGAGAATGGTAGAAAGCAGCAGGGAAATGAAGTGGCCCCCATCTAAGGATTCTGCAACATTTGGATCTCCACTGAGAAGTATGACAGCAGAGGAGTCGGGCTTCATTTCGAAGGGGCATGGCTTTAAGAGAGATAGAGCTGAACTTATACCTAATAGAAGTGGCAGTGCTCCACCAAGTATGGAAGGTTCCTTTTCCGCTATTGAAAACCTGTTATGTCAACAGAACTCCAGCATGAAAACAAGCTTGACTAATCTAAACAATGTTGTGGATAGTGTTGAGAATGAAGAAAATCTGAGTTCAGACCCAGCTCGTTTGGCATACTATTTATCGAATATGAACTTGAATGCAAGGCCACCTCCACCACTTATAGTAAGGGAGAATCATCACCTGGTCCGCCATAGTGGTGGTCTAGGAACCAAATGGAGATCGATTTCTTTAGATGACAGCGCCAGTGGGTCCTTACATCTTTCCCAAGGTTCTCTTTCTACTCTTCAGGAGGACCCTAATGATGCGAGTTCATCCAGAAAATCTCAAGATAACTTGGCAGAAGATAGTGTTGCAGTAATGCCTGTCAAGGACACGGCTTCTTTGGCAAGTTATAATAAGAGTTTAGTGGATCTCATACAGCAAGACTTCCCCCGGACTCCATCTCCTGTATATAATCACTCTCTCTCATCAAGTCTTGGAACAACAGATGAACTAATTGATAGTGATGCACATTCGCTTTCACCAAATGCTTCTTCTCTCGACAAGTCAAAATTACCAGAACCAAATTCAAACTCTATCAATGATTGTCCAGATACAAGTGCCTCGTACACGCTTGCTCTTGGGATCATACCAAATGATGTTCCTTTGCCTACCTCCTCCCCAAGTGCTCAACAATGTCAACCTGATGATGGTACAGGGAACCTACAGAAAGATGAATCTAATATTGGGCATGATGGGTCGAGGAACAATGCATCGATTAATGGTGACCTAGGGTTGGATATTTCCAGTGCGAGAGCATCTAAAGTTGACACCAACAATAACAAGCAACAAGAACTATCTTATGGGAGGTATGTACCACAACACAATTTGTCTACACAACAAGGTGTTCCATATCAACTACAGGGGTTCCAGGCACAATTAGTTTCCCAAGGAATGAATTATTTGCAAAGTGGAATGCAAAATCTTCCCTATAGCTACCCAAAATTCTCTTCTATTGAGACACAACCATCATTGCACTCACCTGGAGTCACACCTGCCTTGTATAGTACACCAACACCATATATGACTTCAGGGAGTCCATTCTACCCGAATTATCAATCATCTGGTGTATTTCCTCCACAATATAGTATGGGCGGATATACTTTAGGTTCTACTTTTCTTCCTTCATATATGCCTGGGTACACTTCTCATGGTTCCTTTTCAATGCCTTTTGATGCTTCTTTGGGGCCAAGATTTAGTGGTCAAACTGTTGATGTTTCAAGAGGGGAGAGAATTCCCCATGAAGGTGATATGCAACACCATAGTAGATTCTATGGGCAACATGGGCCAATGCTCCAGCCACCTTTTGTGGATCCCCTTTATACGCAGTACTATGCTCGTTCTTTGGAGGATTCATATGGTGCTTCAATTCAATATGGTTACTTGTCATCAAGGGGTCAACTTTCGCAACAAGAATTAAATGCTAATGCTTATACGGATGGCCCAAAATTCCACTCTTCAACTAATGGTAATCAGGGTATTCCAAGTCCAAGAAATTTGGGAATCAATGGTAGTGGTTATTACGGACTTCCTTCTGGCATGGGTGTAATGACACAGTTTCCTGCCTCACCTCTTGGTAGTCCAATATTGCCATCATCTCCAATGGGCAGAACGAATCATTTTGGTCGGAAATATGAATTGAGGACTCCTCAAGGTTCTGTTAGTGGAGTATATTCTGGGTGGCAAGGGCTGAGAAACTCCATCTTGGATGATCCTAGAAGACATTCTTTACTTGAAGAACTGAAATCTAGTAATCCTCATAAATTTGAACTTTCCGATATAGCTGAGCGCATTGTTGATTTCAGTGTCGATCAACATGGGAGTCGATTTATTCAACAGAAGCTAGAATACAGCAGTGCTGAAGACAAGGTATCTGTTTTCAAAGAGATTCTTCCAAATGCTTCAAAATTAATGACGGATGTATTTGGAAATTATGTTATTCAAAAGTTTTTTGAATATGGGACTCCTGAGCAGAAAAAGGAGCTTGCAGATCAACTTGCTGGGCAAATGTTGCCTTTAAGTTTGCAGATGTATGGTTGTCGTGTAATCCAGAAGGCTCTCGAAGTTATTGAGCTTGACCAGAAAATACAACTTGTGCATGAGCTTGATGGACATGTTATGATATGTGTACGAGATCAAAATGGAAATCATGTAATACAGAAGTGTATAGAATGCATTCCCACAGAGAAAATTGGATTTATCATTTCTGCTTTTCAAGGACAAGTTGCCACACTTTCTACTCATCCCTATGGCTGTCGTGTCATACAGAGAGTTTTGGAGCATTGTTCGGATGACCTTCAAAGTCAATGTATAGTTGATGAGATCTTGGAATCTGCTTATGATCTTGCTCAAGATCAGTATGGCAATTATGTTACCCAGCATGTTCTGGAACGGGGAAAGCCCAACGAAAGAAGCCAAATTATCAGCAAGTTGGTTGGGAAGATTGTACAATTGAGTCAGCATAAATATGCATCAAATGTTGTTGAGAAGGGTCTGGAGTATGGTGATACTGCTGAGCGGGAGCTCTTGATTGAGGAAATCATTGGACAAATGGAAGAAAACGATAGCCTATTGGCAATGATGAAGGACCAATTTGCCAATTATGTGGTTCAGAAGGTTCTTGAAACAAGCAATGGTAGACAGCGGGAAATACTGCTCAAACTTATAAGAGTTCATATTGATGCTCTGAAGAAATATACTTATGGGAAACACATAGTTGTTCGTTTTCAACAGCTATCCGGTGAAGAGGTCCAAACCTCAGAAGCTGAGGGGGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NABP PUF

Protein Analysis

1021

Amino Acids

112.53

Weight (kDa)

5.58

Isoelectric Point (pI)

45.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NABP	PF07990	491 - 687	1.9e-12	Nucleic acid binding protein NABP
PUF	PF00806	692 - 726	2.2e-10	Pumilio-family RNA binding repeat
PUF	PF00806	731 - 761	5.7e-09	Pumilio-family RNA binding repeat
PUF	PF00806	765 - 797	3.7e-08	Pumilio-family RNA binding repeat
PUF_NOP9	PF22493	795 - 879	3.4e-06	NOP9-like PUF repeat domain
PUF	PF00806	836 - 871	3.3e-13	Pumilio-family RNA binding repeat
PUF	PF00806	875 - 906	5e-08	Pumilio-family RNA binding repeat
PUF	PF00806	910 - 943	1e-08	Pumilio-family RNA binding repeat
PUF	PF00806	953 - 984	2.7e-07	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20250 AT3G20250 AT3G20250
fragaria_vesca	FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910
malus_domestica	MD09G1165700.v1.1 MD17G1161900.v1.1
prunus_persica	Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1
pyrus_communis	pycom07g18900 pycom09g08300 pycom17g15500
rosa_chinensis	RchiOBHm_Chr2g0149341
rosa_laevigata	RLG00000020394
rosa_multiflora	Rmu_sc0000873.1_g000017
rosa_roxburghii	Rroxscaffold_2G00098200
rosa_rugosa	Rorug02G0410600 Rorug02G0410700 Rorug02G0410800
rosa_samantha	Rh2AG471400 Rh2BG483600 Rh2CG457300 Rh2DG492700
rosa_wichuraiana	Rw2G038360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	639, 2951
AarI	CACCTGC	1 cut(s)	1288
Acc36I	ACCTGC	1 cut(s)	1288
AccBSI	CCGCTC	1 cut(s)	2803
AccI	GTMKAC	2 cut(s)	1118, 2923
AciI	CCGC	5 cut(s)	234, 433, 1380, 2803, 2929
AclWI	GGATC	6 cut(s)	109, 663, 878, 1592, 1605, 1991
AcsI	RAATTY	6 cut(s)	811, 1229, 1518, 1733, 1780, 2048
AcuI	CTGAAG	4 cut(s)	515, 1299, 2166, 2993
AfaI	GTAC	8 cut(s)	854, 940, 1101, 1294, 1427, 1616, 2415, 2733
AfiI	CCNNNNNNNGG	4 cut(s)	82, 935, 1021, 1529
AflIII	ACRYGT	1 cut(s)	2398
AjnI	CCWGG	4 cut(s)	426, 1155, 1267, 1420
AjuI	GAANNNNNNNTTGG	6 cut(s)	763, 795, 954, 986, 1219, 1251
Alw21I	GWGCWC	3 cut(s)	208, 916, 2811
Alw26I	GTCTC	2 cut(s)	38, 1238
AlwI	GGATC	6 cut(s)	109, 663, 878, 1592, 1605, 1991
AlwNI	CAGNNNCTG	1 cut(s)	3050
AoxI	GGCC	5 cut(s)	71, 389, 1472, 1571, 1726
ApeKI	GCWGC	1 cut(s)	54
ApoI	RAATTY	6 cut(s)	811, 1229, 1518, 1733, 1780, 2048
AseI	ATTAAT	2 cut(s)	1005, 2195
Asp700I	GAANNNNTTC	2 cut(s)	2008, 2460
AspA2I	CCTAGG	1 cut(s)	1015
AspLEI	GCGC	2 cut(s)	488, 2076
AspS9I	GGNCC	9 cut(s)	72, 430, 495, 538, 1472, 1571, 1727, 2872, 3034
AsuC2I	CCSGG	1 cut(s)	679
AsuHPI	GGTGA	8 cut(s)	416, 759, 1022, 1257, 1544, 1845, 2801, 3038
AsuII	TTCGAA	1 cut(s)	146
AvaII	GGWCC	5 cut(s)	430, 495, 538, 2872, 3034
AvrII	CCTAGG	1 cut(s)	1015
BamHI	GGATCC	1 cut(s)	1597
BanII	GRGCYC	1 cut(s)	2811
BarI	GAAGNNNNNNTAC	2 cut(s)	2117, 2149
BbsI	GAAGAC	2 cut(s)	2011, 2154
Bbv12I	GWGCWC	3 cut(s)	208, 916, 2811
BbvCI	CCTCAGC	1 cut(s)	3051
BbvI	GCAGC	1 cut(s)	66
BceAI	ACGGC	1 cut(s)	636
BcgI	CGANNNNNNTGC	2 cut(s)	114, 148
BciT130I	CCWGG	4 cut(s)	428, 1157, 1269, 1422
BcnI	CCSGG	1 cut(s)	679
BcoDI	GTCTC	2 cut(s)	38, 1238
BfaI	CTAG	5 cut(s)	449, 1016, 2001, 2034, 2129
BfmI	CTRYAG	3 cut(s)	950, 1145, 1216
BfoI	RGCGCY	1 cut(s)	489
BfuAI	ACCTGC	1 cut(s)	1288
BglII	AGATCT	1 cut(s)	2607
BisI	GCNGC	1 cut(s)	55
BlnI	CCTAGG	1 cut(s)	1015
BlpI	GCTNAGC	2 cut(s)	2070, 2799
BlsI	GCNGC	1 cut(s)	56
BmcAI	AGTACT	1 cut(s)	1616
Bme1390I	CCNGG	5 cut(s)	428, 679, 1157, 1269, 1422
Bme18I	GGWCC	5 cut(s)	430, 495, 538, 2872, 3034
BmgT120I	GGNCC	9 cut(s)	72, 430, 495, 538, 1472, 1571, 1727, 2872, 3034
BmrFI	CCNGG	5 cut(s)	428, 679, 1157, 1269, 1422
BmsI	GCATC	7 cut(s)	538, 742, 1007, 1052, 1450, 2765, 2956
BpiI	GAAGAC	2 cut(s)	2011, 2154
BplI	GAGNNNNNCTC	2 cut(s)	1918, 1950
BpmI	CTGGAG	4 cut(s)	250, 1290, 1565, 2801
Bpu10I	CCTNAGC	2 cut(s)	2253, 3051
Bpu1102I	GCTNAGC	2 cut(s)	2070, 2799
Bpu14I	TTCGAA	1 cut(s)	146
BpuEI	CTTGAG	3 cut(s)	555, 1920, 2619
BpuMI	CCSGG	1 cut(s)	679
Bsa29I	ATCGAT	2 cut(s)	467, 1001
BsaI	GGTCTC	1 cut(s)	38
BsaJI	CCNNGG	6 cut(s)	27, 511, 677, 1015, 1174, 1421
BsaWI	WCCGGW	1 cut(s)	3023
BsaXI	ACNNNNNCTCC	2 cut(s)	673, 703
Bsc4I	CCNNNNNNNGG	4 cut(s)	82, 935, 1021, 1529
Bse1I	ACTGG	2 cut(s)	489, 1032
Bse3DI	GCAATG	3 cut(s)	1256, 2868, 2923
BseBI	CCWGG	4 cut(s)	428, 1157, 1269, 1422
BseCI	ATCGAT	2 cut(s)	467, 1001
BseDI	CCNNGG	6 cut(s)	27, 511, 677, 1015, 1174, 1421
BseGI	GGATG	7 cut(s)	24, 557, 1729, 1999, 2209, 2527, 2581
BseLI	CCNNNNNNNGG	4 cut(s)	82, 935, 1021, 1529
BseMI	GCAATG	3 cut(s)	1256, 2868, 2923
BseMII	CTCAG	8 cut(s)	101, 323, 1967, 2061, 2244, 2790, 3042, 3057
BseNI	ACTGG	2 cut(s)	489, 1032
BseRI	GAGGAG	3 cut(s)	143, 892, 1923
BseXI	GCAGC	1 cut(s)	66
BseYI	CCCAGC	3 cut(s)	343, 2285, 2661
BshFI	GGCC	5 cut(s)	73, 391, 1474, 1573, 1728
BshVI	ATCGAT	2 cut(s)	467, 1001
BsiHKAI	GWGCWC	3 cut(s)	208, 916, 2811
BsiSI	CCGG	2 cut(s)	679, 3024
BslFI	GGGAC	1 cut(s)	2260
BslI	CCNNNNNNNGG	4 cut(s)	82, 935, 1021, 1529
BsmAI	GTCTC	2 cut(s)	38, 1238
BsmFI	GGGAC	1 cut(s)	2260
BsmI	GAATGC	4 cut(s)	388, 1206, 2460, 2462
BsnI	GGCC	5 cut(s)	73, 391, 1474, 1573, 1728
Bso31I	GGTCTC	1 cut(s)	38
Bsp119I	TTCGAA	1 cut(s)	146
Bsp1286I	GDGCHC	3 cut(s)	208, 916, 2811
Bsp1407I	TGTACA	1 cut(s)	2731
Bsp1720I	GCTNAGC	2 cut(s)	2070, 2799
BspACI	CCGC	5 cut(s)	234, 433, 1380, 2803, 2929
BspANI	GGCC	5 cut(s)	73, 391, 1474, 1573, 1728
BspCNI	CTCAG	8 cut(s)	102, 324, 1968, 2062, 2245, 2791, 3043, 3056
BspDI	ATCGAT	2 cut(s)	467, 1001
BspHI	TCATGA	1 cut(s)	36
BspMI	ACCTGC	1 cut(s)	1288
BspPI	GGATC	6 cut(s)	109, 663, 878, 1592, 1605, 1991
BspT104I	TTCGAA	1 cut(s)	146
BspTNI	GGTCTC	1 cut(s)	38
BsrBI	CCGCTC	1 cut(s)	2803
BsrDI	GCAATG	3 cut(s)	1256, 2868, 2923
BsrGI	TGTACA	1 cut(s)	2731
BsrI	ACTGG	2 cut(s)	489, 1032
BssECI	CCNNGG	6 cut(s)	27, 511, 677, 1015, 1174, 1421
BssT1I	CCWWGG	3 cut(s)	511, 1015, 1174
Bst2UI	CCWGG	4 cut(s)	428, 1157, 1269, 1422
Bst4CI	ACNGT	2 cut(s)	1495, 1842
Bst6I	CTCTTC	4 cut(s)	534, 1240, 1747, 3024
BstAPI	GCANNNNNTGC	1 cut(s)	2296
BstAUI	TGTACA	1 cut(s)	2731
BstBI	TTCGAA	1 cut(s)	146
BstC8I	GCNNGC	2 cut(s)	861, 2271
BstDEI	CTNAG	9 cut(s)	81, 110, 332, 1976, 2070, 2253, 2799, 3043, 3051
BstEII	GGTNACC	1 cut(s)	1010
BstF5I	GGATG	7 cut(s)	24, 557, 1729, 1999, 2209, 2527, 2581
BstH2I	RGCGCY	1 cut(s)	489
BstHHI	GCGC	2 cut(s)	488, 2076
BstMAI	GTCTC	2 cut(s)	38, 1238
BstMWI	GCNNNNNNNGC	4 cut(s)	353, 629, 1570, 2296
BstNI	CCWGG	4 cut(s)	428, 1157, 1269, 1422
BstNSI	RCATGY	2 cut(s)	2402, 2669
BstPI	GGTNACC	1 cut(s)	1010
BstSCI	CCNGG	5 cut(s)	426, 677, 1155, 1267, 1420
BstSFI	CTRYAG	3 cut(s)	950, 1145, 1216
BstV1I	GCAGC	1 cut(s)	66
BstV2I	GAAGAC	2 cut(s)	2011, 2154
BstX2I	RGATCY	4 cut(s)	101, 655, 1597, 2607
BstXI	CCANNNNNNTGG	2 cut(s)	443, 2890
BstYI	RGATCY	4 cut(s)	101, 655, 1597, 2607
Bsu15I	ATCGAT	2 cut(s)	467, 1001
BsuRI	GGCC	5 cut(s)	73, 391, 1474, 1573, 1728
BsuTUI	ATCGAT	2 cut(s)	467, 1001
BtsCI	GGATG	7 cut(s)	24, 557, 1729, 1999, 2209, 2527, 2581
BtsI	GCAGTG	2 cut(s)	208, 2146
BtsIMutI	CAGTG	6 cut(s)	107, 208, 496, 1039, 2095, 2146
BveI	ACCTGC	1 cut(s)	1288
Cac8I	GCNNGC	2 cut(s)	861, 2271
CaiI	CAGNNNCTG	1 cut(s)	3050
CciI	TCATGA	1 cut(s)	36
CfoI	GCGC	2 cut(s)	488, 2076
Cfr13I	GGNCC	9 cut(s)	72, 430, 495, 538, 1472, 1571, 1727, 2872, 3034
ClaI	ATCGAT	2 cut(s)	467, 1001
CsiI	ACCWGGT	1 cut(s)	426
Csp6I	GTAC	8 cut(s)	853, 939, 1100, 1293, 1426, 1615, 2414, 2732
CspCI	CAANNNNNGTGG	2 cut(s)	1780, 1815
CviQI	GTAC	8 cut(s)	853, 939, 1100, 1293, 1426, 1615, 2414, 2732
DdeI	CTNAG	9 cut(s)	81, 110, 332, 1976, 2070, 2253, 2799, 3043, 3051
Eam1104I	CTCTTC	4 cut(s)	534, 1240, 1747, 3024
EarI	CTCTTC	4 cut(s)	534, 1240, 1747, 3024
EciI	GGCGGA	2 cut(s)	422, 1395
Ecl136II	GAGCTC	1 cut(s)	2809
Eco130I	CCWWGG	3 cut(s)	511, 1015, 1174
Eco24I	GRGCYC	1 cut(s)	2811
Eco31I	GGTCTC	1 cut(s)	38
Eco47I	GGWCC	5 cut(s)	430, 495, 538, 2872, 3034
Eco53kI	GAGCTC	1 cut(s)	2809
Eco57I	CTGAAG	4 cut(s)	515, 1299, 2166, 2993
Eco91I	GGTNACC	1 cut(s)	1010
EcoICRI	GAGCTC	1 cut(s)	2809
EcoO109I	RGGNCCY	2 cut(s)	495, 538
EcoO65I	GGTNACC	1 cut(s)	1010
EcoRI	GAATTC	1 cut(s)	1518
EcoRII	CCWGG	4 cut(s)	426, 1155, 1267, 1420
EcoT14I	CCWWGG	3 cut(s)	511, 1015, 1174
EcoT22I	ATGCAT	4 cut(s)	6, 1000, 2462, 2758
EcoT38I	GRGCYC	1 cut(s)	2811
ErhI	CCWWGG	3 cut(s)	511, 1015, 1174
FalI	AAGNNNNNCTT	6 cut(s)	504, 536, 1996, 2028, 2160, 2192
FaqI	GGGAC	1 cut(s)	2260
FauI	CCCGC	2 cut(s)	2796, 2922
FauNDI	CATATG	1 cut(s)	1642
FblI	GTMKAC	2 cut(s)	1118, 2923
Fnu4HI	GCNGC	1 cut(s)	55
FokI	GGATG	7 cut(s)	31, 544, 1736, 2006, 2216, 2514, 2588
FriOI	GRGCYC	1 cut(s)	2811
Fsp4HI	GCNGC	1 cut(s)	55
FspBI	CTAG	5 cut(s)	449, 1016, 2001, 2034, 2129
GlaI	GCGC	2 cut(s)	487, 2075
GluI	GCNGC	1 cut(s)	55
GsaI	CCCAGC	3 cut(s)	347, 2289, 2665
GsuI	CTGGAG	4 cut(s)	250, 1290, 1565, 2801
HaeII	RGCGCY	1 cut(s)	489
HaeIII	GGCC	5 cut(s)	73, 391, 1474, 1573, 1728
HapII	CCGG	2 cut(s)	679, 3024
HhaI	GCGC	2 cut(s)	488, 2076
Hin6I	GCGC	2 cut(s)	486, 2074
HinP1I	GCGC	2 cut(s)	486, 2074
HincII	GTYRAC	4 cut(s)	257, 926, 1054, 1685
HindII	GTYRAC	4 cut(s)	257, 926, 1054, 1685
HindIII	AAGCTT	1 cut(s)	280
HpaII	CCGG	2 cut(s)	679, 3024
HphI	GGTGA	8 cut(s)	416, 759, 1022, 1257, 1544, 1845, 2801, 3038
Hpy188I	TCNGA	8 cut(s)	333, 340, 1914, 2062, 2575, 2898, 2973, 3046
HpyCH4III	ACNGT	2 cut(s)	1495, 1842
HpyF10VI	GCNNNNNNNGC	4 cut(s)	353, 629, 1570, 2296
HpyF3I	CTNAG	9 cut(s)	81, 110, 332, 1976, 2070, 2253, 2799, 3043, 3051
HspAI	GCGC	2 cut(s)	486, 2074
LmnI	GCTCC	5 cut(s)	211, 1584, 2266, 2563, 2806
Lsp1109I	GCAGC	1 cut(s)	66
LweI	GCATC	7 cut(s)	538, 742, 1007, 1052, 1450, 2765, 2956
MabI	ACCWGGT	1 cut(s)	426
MaeI	CTAG	5 cut(s)	449, 1016, 2001, 2034, 2129
MaeIII	GTNAC	4 cut(s)	1010, 1273, 1664, 2656
MbiI	CCGCTC	1 cut(s)	2803
MfeI	CAATTG	1 cut(s)	2735
MflI	RGATCY	4 cut(s)	101, 655, 1597, 2607
MhlI	GDGCHC	3 cut(s)	208, 916, 2811
MlyI	GAGTC	8 cut(s)	140, 676, 1281, 1330, 1924, 2116, 2242, 2749
MmeI	TCCRAC	2 cut(s)	1002, 1892
Mph1103I	ATGCAT	4 cut(s)	6, 1000, 2462, 2758
MroXI	GAANNNNTTC	2 cut(s)	2008, 2460
MseI	TTAA	5 cut(s)	162, 1005, 1703, 2195, 2303
MslI	CAYNNNNRTG	6 cut(s)	41, 441, 1530, 2532, 2751, 2964
MspA1I	CMGCKG	1 cut(s)	2929
MspI	CCGG	2 cut(s)	679, 3024
MspR9I	CCNGG	5 cut(s)	428, 679, 1157, 1269, 1422
MunI	CAATTG	1 cut(s)	2735
Mva1269I	GAATGC	4 cut(s)	388, 1206, 2460, 2462
MvaI	CCWGG	4 cut(s)	428, 1157, 1269, 1422
MwoI	GCNNNNNNNGC	4 cut(s)	353, 629, 1570, 2296
NciI	CCSGG	1 cut(s)	679
NdeI	CATATG	1 cut(s)	1642
NmuCI	GTSAC	2 cut(s)	1010, 1273
NsiI	ATGCAT	4 cut(s)	6, 1000, 2462, 2758
NspI	RCATGY	2 cut(s)	2402, 2669
NspV	TTCGAA	1 cut(s)	146
PagI	TCATGA	1 cut(s)	36
PaqCI	CACCTGC	1 cut(s)	1288
PciI	ACATGT	1 cut(s)	2398
PctI	GAATGC	4 cut(s)	388, 1206, 2460, 2462
PdmI	GAANNNNTTC	2 cut(s)	2008, 2460
PfeI	GAWTC	9 cut(s)	86, 418, 962, 1554, 1637, 1788, 1900, 2173, 2615
PkrI	GCNGC	1 cut(s)	56
PleI	GAGTC	8 cut(s)	139, 676, 1280, 1329, 1924, 2115, 2242, 2748
PpsI	GAGTC	8 cut(s)	139, 676, 1280, 1329, 1924, 2115, 2242, 2748
PpuMI	RGGWCCY	2 cut(s)	495, 538
PscI	ACATGT	1 cut(s)	2398
PshBI	ATTAAT	2 cut(s)	1005, 2195
PsiI	TTATAA	2 cut(s)	639, 2951
Psp124BI	GAGCTC	1 cut(s)	2811
Psp5II	RGGWCCY	2 cut(s)	495, 538
Psp6I	CCWGG	4 cut(s)	426, 1155, 1267, 1420
PspEI	GGTNACC	1 cut(s)	1010
PspFI	CCCAGC	3 cut(s)	343, 2285, 2661
PspGI	CCWGG	4 cut(s)	426, 1155, 1267, 1420
PspPI	GGNCC	9 cut(s)	72, 430, 495, 538, 1472, 1571, 1727, 2872, 3034
PspPPI	RGGWCCY	2 cut(s)	495, 538
PsrI	GAACNNNNNNTAC	2 cut(s)	208, 240
PstNI	CAGNNNCTG	1 cut(s)	3050
PsuI	RGATCY	4 cut(s)	101, 655, 1597, 2607
RsaI	GTAC	8 cut(s)	854, 940, 1101, 1294, 1427, 1616, 2415, 2733
RsaNI	GTAC	8 cut(s)	853, 939, 1100, 1293, 1426, 1615, 2414, 2732
RseI	CAYNNNNRTG	6 cut(s)	41, 441, 1530, 2532, 2751, 2964
SacI	GAGCTC	1 cut(s)	2811
SaqAI	TTAA	5 cut(s)	162, 1005, 1703, 2195, 2303
SatI	GCNGC	1 cut(s)	55
Sau96I	GGNCC	9 cut(s)	72, 430, 495, 538, 1472, 1571, 1727, 2872, 3034
ScaI	AGTACT	1 cut(s)	1616
SchI	GAGTC	8 cut(s)	140, 676, 1281, 1330, 1924, 2116, 2242, 2749
ScrFI	CCNGG	5 cut(s)	428, 679, 1157, 1269, 1422
SduI	GDGCHC	3 cut(s)	208, 916, 2811
SexAI	ACCWGGT	1 cut(s)	426
SfaNI	GCATC	7 cut(s)	538, 742, 1007, 1052, 1450, 2765, 2956
SfcI	CTRYAG	3 cut(s)	950, 1145, 1216
SfuI	TTCGAA	1 cut(s)	146
SinI	GGWCC	5 cut(s)	430, 495, 538, 2872, 3034
SmiMI	CAYNNNNRTG	6 cut(s)	41, 441, 1530, 2532, 2751, 2964
SmlI	CTYRAG	3 cut(s)	570, 1935, 2634
SmoI	CTYRAG	3 cut(s)	570, 1935, 2634
SsiI	CCGC	5 cut(s)	234, 433, 1380, 2803, 2929
SspI	AATATT	2 cut(s)	970, 1872
SspMI	CTAG	5 cut(s)	449, 1016, 2001, 2034, 2129
SstI	GAGCTC	1 cut(s)	2811
StyD4I	CCNGG	5 cut(s)	426, 677, 1155, 1267, 1420
StyI	CCWWGG	3 cut(s)	511, 1015, 1174
TaaI	ACNGT	2 cut(s)	1495, 1842
TaqI	TCGA	9 cut(s)	146, 368, 467, 786, 986, 1001, 2094, 2110, 2346
TatI	WGTACW	3 cut(s)	1292, 1614, 2731
TfiI	GAWTC	9 cut(s)	86, 418, 962, 1554, 1637, 1788, 1900, 2173, 2615
Tru1I	TTAA	5 cut(s)	162, 1005, 1703, 2195, 2303
Tru9I	TTAA	5 cut(s)	162, 1005, 1703, 2195, 2303
TscAI	CASTG	6 cut(s)	114, 208, 496, 1039, 2095, 2146
TseFI	GTSAC	2 cut(s)	1010, 1273
TseI	GCWGC	1 cut(s)	54
Tsp45I	GTSAC	2 cut(s)	1010, 1273
TspGWI	ACGGA	3 cut(s)	1736, 1824, 2216
TspRI	CASTG	6 cut(s)	114, 208, 496, 1039, 2095, 2146
VpaK11BI	GGWCC	5 cut(s)	430, 495, 538, 2872, 3034
VspI	ATTAAT	2 cut(s)	1005, 2195
XapI	RAATTY	6 cut(s)	811, 1229, 1518, 1733, 1780, 2048
XceI	RCATGY	2 cut(s)	2402, 2669
XmaJI	CCTAGG	1 cut(s)	1015
XmiI	GTMKAC	2 cut(s)	1118, 2923
XmnI	GAANNNNTTC	2 cut(s)	2008, 2460
XspI	CTAG	5 cut(s)	449, 1016, 2001, 2034, 2129
ZrmI	AGTACT	1 cut(s)	1616
Zsp2I	ATGCAT	4 cut(s)	6, 1000, 2462, 2758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.