Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0004599

Physical Location & Seq

Genomic Coordinates Forward (+)

13550303 .. 13557840

Gene Structure 7538 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1165700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3048 bp

ATGGCAACTGAGGGTCTCCTGAGAATGGTAGAAAGCAGCAGGGGAAAGAAGTGGCCCCCATCTAAGGATGCTGCAACATTTGGATCTCCACTGAGAAGTATGACAGCAGAGGAGTCGGGCTTCGTTTCAAAGGGGCATGGCTTTAAGAGAGAGAGAGCTGAAGTTATACCCAGTCGAAGTGGTAGTGCTCCACCAAGTATGGAAGGTTCCTTTTCCTCTATTGAAAACCTACTAAGTCAACAGAACTCCAGCATGAAAACAAGCTTGACTAATTTAAACAACACTCTGGATAATGTTGAGAATGAAGAAAAACTGCGTTCAGACCCAGCTTATATGGCATACTACTTATCAAATATGAACTTGAATGCAAAGCCGCCTCCACCACTTACTGTACGGGAGAATCATCATGTCGTCCGCCATAGTGGTGGTCTAGGAACCAAAGGGAGACTGACTTCTTTAGATGACAGCGCCAGTGGGTCCTTGCATCTTTCTCAAGGTTCTCTCTCAACTCTTCAGGAGGACCCTAATGATGCTAGTTCATTCAGAAACTCTAATGATAACTTGGCAGAAGATAGTGTTGCAGTAATGCCTGTCAAGGACACGGTTTCTTTGGCAAGTTATAATAAGAGTCTATTGGATCTCATACAGCAAGACTTCCCCCGGACTCCATCTCCTGTATATAATCACTCCCTCACATCAAGTCTTGGAACAACAGATGAACCAATTGATAGTGATGTAAATTCACTTTCACCAAATGCTTCTTCTGTCAACAAGTCAAAATTACCTGAACCAAATTCAAACTCTATCAATGATTGTCCAGATACAAGTGCCTCGGACGCGCTTGCTCGTGGGTTCATACCAAATGATGTTCCTTTGCCTACCACCTCCCCAAGTGCTCAACATCGTGATGTTACAGGGAACCTACAGAAAGATGAATCTAACATTGAGCATGGTGGGTCGGGGAATAATGCATCGATTAATGGTGACCTTCGGTTGGATCTTTCCAGAGCGAGAGCAGCTAAAGTTGATACCAACAATAACAATCAACAAGAACAATCTTATGGGAGGTATGTACCACAGCACAATTTGTCTACACAACAAGGGGTTCCATATCAACTTCAGGGGTTCCAGGCACAATTAGTTTCACAAGGAATGAATCATTTGCAAAGCAGAATGCAAAATCTTCCCCACGGCTACCCAAAATTCTCTTCTATTGAGACACAACCATCATTGCACTCACCTGGTGTCACACCTCCCTTGTATGGTACAACCACAGCATATATGACTTCAGGAAGTCCATTCTACTCAAATTATCAGTCGTCTGGTGCGTTTCCTACACAATATGGAATGGGTGGATATGCTTTAGGTTCTACTTATCTTCCTTCATATATGCCTGGGTATGCTTCTCATGGGTCTTTTCAAATGCTCTTTGATGCTACTTTGGGGCCAAGTTTCAATGGTCGAACTGCTGATGTTTCAAGAGGGGAGAGGATTCCCCATGAAGGTGATATGCAACACCCCAGTAGATCTTATGGGCAACATGGGCCAGTGCTCCAGCCACCTTTTGTGGATCCCCTTTATACGCAATACTATCCTCGTCTGTTGGAGGATTCATATGGTGCTTCAATTCCTTATGGTCACTTGGCGTCAAGGGCTGTTGTTAGAGGCCAACTTTCGCAACAAGAATTAAATGTTAATGCTTATAGGGCTGACCCAAAATTCCACACTTCAACTAATGGTAATCTGGGTATTCTAAGTCCAAGGAGTTTGGGAAACAATGGTAGTGGTTATTATGGACATCCTTCTGGCATGGGTGTAGTGACACAGTTTCCAGCCTCACATCTTGGTAGTCCAATATTGCCATCATCTCCAATGGGCAGAATGAATCATCTTGGTCGGAAATATGAATTGAGGTCTCCTGAAGGTTCTGTTAGTGGAGTATATTCTGGGTGGCAAGGGCAGAGAAGCTCCATCTTGGATGATCCTAGACGACACTCTTTACTTGAAGAACTGAAATCTAGTAATCCCCGTAAATTTGAACTTTCTGATATAGCTGGGCGCATTGTTGATTTCAGTGTCGATCAACATGGGAGTCGATTTATTCAACAGAAGCTAGAGTACTGCAGTTCTGAAAACAAGGCATCTGTTTTCAAAGAGATTCTTCCACGTGCTTCAAAATTAATGACGGATGTGTTTGGGAATTATGTTATTCAAAAGTTTTTTGAATATGGGAGTCCTGAGCAGAAAAAGGAGCTTGCAGATCAACTTGCTGGGCAAGTGTTGCCCTTAAGTTTGCAGATGTATGGTTGTCGTGTAATCCAGAAGGCTCTCGAAGTTATTGAGCTTGACCAGAAAATACAACTTGTGCATGAGCTTGATGGACATGTCATGACGTGTGTACGAGATCAAAATGGAAATCATGTAATACAGAAGTGTATAGAATGCATTCCCACAGAGAAAATTGGATTTATCATTTCTGCTTTCCAAGGACAAGTTGCCACACTTTCTACTCATCCCTATGGCTGTCGTGTCATACAGAGAGTTTTGGAGCATTGTTCGGATGACCTTCAAAGTCAGTGTATAGTTGATGAGATCTTGGAATCTACTTATGATCTTGCTCAAGATCAGTATGGCAATTATGTTACCCAGCATGTTCTGGAAAGGGGAAAGCCCAACGAAAGAAGCCAAATTATCACCAAGTTGGTTGGGAAGATTGTACGATTGAGTCAGCATAAATATGCATCAAATGTTGTTGAGAAGTGTCTGGAGTATGGTGATACTGCTGAGCGGGAGCTCTTGATTGAGGAAATCATTGGACAAATGGAAGAAAATGATAGCCTGTTGCCAATGATGAAGGACCAATTTGCCAATTATGTGGTTCAGAAGGTTCTTGAAACAAGCAATGATAGACAGCGGGAAATACTGCTCAAACTTATAAGAGTTCATATTGATGCTCTGAAGAAATATACTTATGGGAAACACATAGTTGTTCGGTTTCAACAGCTATCCGGTGAAGATGTCCAAACCTCAGAAGCTGAGGGGGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006417 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009615 GO:0009628 GO:0009651 GO:0009819 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009894 GO:0009896 GO:0010035 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010608 GO:0010629 GO:0017148 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031329 GO:0031331 GO:0032268 GO:0032269 GO:0034248 GO:0034249 GO:0042221 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043487 GO:0043488 GO:0044424 GO:0044444 GO:0044464 GO:0045935 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050779 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051252 GO:0051254 GO:0051607 GO:0051704 GO:0051707 GO:0060255 GO:0061013 GO:0061014 GO:0061157 GO:0061158 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:0098542 GO:1901363 GO:1901700 GO:1903311 GO:1903313 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NABP PUF

Protein Analysis

1016

Amino Acids

111.93

Weight (kDa)

6.03

Isoelectric Point (pI)

46.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NABP	PF07990	434 - 682	8.3e-13	Nucleic acid binding protein NABP
PUF	PF00806	687 - 721	2.6e-11	Pumilio-family RNA binding repeat
PUF	PF00806	726 - 756	9e-09	Pumilio-family RNA binding repeat
PUF	PF00806	759 - 792	1.5e-08	Pumilio-family RNA binding repeat
PUF_NOP9	PF22493	790 - 866	1.9e-06	NOP9-like PUF repeat domain
PUF	PF00806	831 - 866	3.3e-13	Pumilio-family RNA binding repeat
PUF	PF00806	870 - 901	9.9e-08	Pumilio-family RNA binding repeat
PUF	PF00806	905 - 938	6.6e-11	Pumilio-family RNA binding repeat
PUF	PF00806	949 - 979	3.6e-07	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004599)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20250 AT3G20250 AT3G20250
fragaria_vesca	FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910 FvH4_6g36910
malus_domestica	MD09G1165700.v1.1 MD17G1161900.v1.1
prunus_persica	Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1 Prupe.3G023300_v2.0.a1
pyrus_communis	pycom07g18900 pycom09g08300 pycom17g15500
rosa_chinensis	RchiOBHm_Chr2g0149341
rosa_laevigata	RLG00000020394
rosa_multiflora	Rmu_sc0000873.1_g000017
rosa_roxburghii	Rroxscaffold_2G00098200
rosa_rugosa	Rorug02G0410600 Rorug02G0410700 Rorug02G0410800
rosa_samantha	Rh2AG471400 Rh2BG483600 Rh2CG457300 Rh2DG492700
rosa_wichuraiana	Rw2G038360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	621, 2936
AccBSI	CCGCTC	1 cut(s)	2788
AccI	GTMKAC	1 cut(s)	1091
AccII	CGCG	1 cut(s)	839
AciI	CCGC	4 cut(s)	374, 415, 2788, 2914
AclWI	GGATC	6 cut(s)	91, 645, 1005, 1565, 1578, 1976
AcsI	RAATTY	5 cut(s)	739, 793, 1202, 1718, 2033
AcuI	CTGAAG	6 cut(s)	180, 497, 1103, 1272, 1941, 2978
AcvI	CACGTG	1 cut(s)	2168
AcyI	GRCGYC	1 cut(s)	1646
AdeI	CACNNNGTG	1 cut(s)	1244
AfaI	GTAC	6 cut(s)	393, 1074, 1267, 2120, 2400, 2718
AfiI	CCNNNNNNNGG	5 cut(s)	25, 64, 994, 1262, 1502
AflII	CTTAAG	1 cut(s)	2287
AflIII	ACRYGT	2 cut(s)	2383, 2393
AjiI	CACGTC	1 cut(s)	2394
AjnI	CCWGG	3 cut(s)	1128, 1240, 1393
AjuI	GAANNNNNNNTTGG	4 cut(s)	745, 777, 1192, 1224
Alw21I	GWGCWC	4 cut(s)	190, 898, 1554, 2796
Alw26I	GTCTC	4 cut(s)	20, 439, 1211, 1920
AlwI	GGATC	6 cut(s)	91, 645, 1005, 1565, 1578, 1976
AlwNI	CAGNNNCTG	1 cut(s)	3035
AoxI	GGCC	4 cut(s)	53, 1445, 1544, 1666
ApeKI	GCWGC	3 cut(s)	36, 71, 1016
ApoI	RAATTY	5 cut(s)	739, 793, 1202, 1718, 2033
ArsI	GACNNNNNNTTYG	2 cut(s)	1301, 1333
AseI	ATTAAT	2 cut(s)	978, 2180
Asp700I	GAANNNNTTC	1 cut(s)	2445
AspLEI	GCGC	3 cut(s)	470, 841, 2061
AspS9I	GGNCC	6 cut(s)	54, 477, 520, 1445, 1544, 2857
AsuC2I	CCSGG	1 cut(s)	661
AsuHPI	GGTGA	7 cut(s)	741, 995, 1230, 1517, 2686, 2786, 3023
AvaII	GGWCC	3 cut(s)	477, 520, 2857
BamHI	GGATCC	1 cut(s)	1570
BanII	GRGCYC	1 cut(s)	2796
BarI	GAAGNNNNNNTAC	2 cut(s)	2102, 2134
BauI	CACGAG	1 cut(s)	846
BbrPI	CACGTG	1 cut(s)	2168
Bbv12I	GWGCWC	4 cut(s)	190, 898, 1554, 2796
BbvCI	CCTCAGC	1 cut(s)	3036
BbvI	GCAGC	3 cut(s)	48, 58, 1028
BccI	CCATC	6 cut(s)	67, 676, 1234, 1870, 1979, 2372
BceAI	ACGGC	1 cut(s)	1207
BcgI	CGANNNNNNTGC	2 cut(s)	96, 130
BciT130I	CCWGG	3 cut(s)	1130, 1242, 1395
BcnI	CCSGG	1 cut(s)	661
BcoDI	GTCTC	4 cut(s)	20, 439, 1211, 1920
BfaI	CTAG	5 cut(s)	431, 534, 1986, 2019, 2114
BfmI	CTRYAG	2 cut(s)	923, 2122
BfoI	RGCGCY	1 cut(s)	471
BfrI	CTTAAG	1 cut(s)	2287
BglII	AGATCT	2 cut(s)	1526, 2592
BisI	GCNGC	4 cut(s)	37, 72, 374, 1017
BlpI	GCTNAGC	1 cut(s)	2784
BlsI	GCNGC	4 cut(s)	38, 73, 375, 1018
BmcAI	AGTACT	1 cut(s)	2120
Bme1390I	CCNGG	4 cut(s)	661, 1130, 1242, 1395
Bme18I	GGWCC	3 cut(s)	477, 520, 2857
BmgBI	CACGTC	1 cut(s)	2394
BmgT120I	GGNCC	6 cut(s)	54, 477, 520, 1445, 1544, 2857
BmrFI	CCNGG	4 cut(s)	661, 1130, 1242, 1395
BmrI	ACTGGG	2 cut(s)	165, 1515
BmsI	GCATC	8 cut(s)	58, 493, 520, 980, 1423, 2150, 2750, 2941
BmuI	ACTGGG	2 cut(s)	165, 1515
BpmI	CTGGAG	3 cut(s)	232, 1538, 2786
Bpu10I	CCTNAGC	2 cut(s)	2238, 3036
Bpu1102I	GCTNAGC	1 cut(s)	2784
BpuEI	CTTGAG	2 cut(s)	477, 2604
BpuMI	CCSGG	1 cut(s)	661
Bsa29I	ATCGAT	1 cut(s)	974
BsaAI	YACGTR	1 cut(s)	2168
BsaHI	GRCGYC	1 cut(s)	1646
BsaI	GGTCTC	2 cut(s)	20, 1920
BsaJI	CCNNGG	6 cut(s)	659, 831, 1189, 1394, 1760, 2485
BsaWI	WCCGGW	1 cut(s)	3008
BsaXI	ACNNNNNCTCC	2 cut(s)	655, 685
Bsc4I	CCNNNNNNNGG	5 cut(s)	25, 64, 994, 1262, 1502
Bse1I	ACTGG	4 cut(s)	171, 471, 1521, 1547
Bse3DI	GCAATG	2 cut(s)	1229, 2908
BseBI	CCWGG	3 cut(s)	1130, 1242, 1395
BseCI	ATCGAT	1 cut(s)	974
BseDI	CCNNGG	6 cut(s)	659, 831, 1189, 1394, 1760, 2485
BseGI	GGATG	6 cut(s)	73, 1798, 1984, 2194, 2512, 2566
BseLI	CCNNNNNNNGG	5 cut(s)	25, 64, 994, 1262, 1502
BseMI	GCAATG	2 cut(s)	1229, 2908
BseMII	CTCAG	6 cut(s)	11, 83, 2229, 2775, 3027, 3042
BseNI	ACTGG	4 cut(s)	171, 471, 1521, 1547
BseRI	GAGGAG	1 cut(s)	125
BseXI	GCAGC	3 cut(s)	48, 58, 1028
BseYI	CCCAGC	4 cut(s)	325, 2054, 2270, 2646
Bsh1236I	CGCG	1 cut(s)	839
BshFI	GGCC	4 cut(s)	55, 1447, 1546, 1668
BshVI	ATCGAT	1 cut(s)	974
BsiHKAI	GWGCWC	4 cut(s)	190, 898, 1554, 2796
BsiSI	CCGG	2 cut(s)	661, 3009
BslI	CCNNNNNNNGG	5 cut(s)	25, 64, 994, 1262, 1502
BsmAI	GTCTC	4 cut(s)	20, 439, 1211, 1920
BsmI	GAATGC	4 cut(s)	370, 1179, 2445, 2447
BsnI	GGCC	4 cut(s)	55, 1447, 1546, 1668
Bso31I	GGTCTC	2 cut(s)	20, 1920
Bsp1286I	GDGCHC	4 cut(s)	190, 898, 1554, 2796
Bsp1720I	GCTNAGC	1 cut(s)	2784
BspACI	CCGC	4 cut(s)	374, 415, 2788, 2914
BspANI	GGCC	4 cut(s)	55, 1447, 1546, 1668
BspCNI	CTCAG	6 cut(s)	12, 84, 2230, 2776, 3028, 3041
BspDI	ATCGAT	1 cut(s)	974
BspFNI	CGCG	1 cut(s)	839
BspHI	TCATGA	1 cut(s)	2388
BspMAI	CTGCAG	1 cut(s)	2126
BspPI	GGATC	6 cut(s)	91, 645, 1005, 1565, 1578, 1976
BspTI	CTTAAG	1 cut(s)	2287
BspTNI	GGTCTC	2 cut(s)	20, 1920
BsrBI	CCGCTC	1 cut(s)	2788
BsrDI	GCAATG	2 cut(s)	1229, 2908
BsrI	ACTGG	4 cut(s)	171, 471, 1521, 1547
BssECI	CCNNGG	6 cut(s)	659, 831, 1189, 1394, 1760, 2485
BssNI	GRCGYC	1 cut(s)	1646
BssSI	CACGAG	1 cut(s)	846
BssT1I	CCWWGG	2 cut(s)	1760, 2485
Bst2BI	CACGAG	1 cut(s)	846
Bst2UI	CCWGG	3 cut(s)	1130, 1242, 1395
Bst4CI	ACNGT	3 cut(s)	391, 604, 1827
Bst6I	CTCTTC	2 cut(s)	516, 1213
BstACI	GRCGYC	1 cut(s)	1646
BstAFI	CTTAAG	1 cut(s)	2287
BstAPI	GCANNNNNTGC	1 cut(s)	2281
BstBAI	YACGTR	1 cut(s)	2168
BstC8I	GCNNGC	2 cut(s)	843, 2256
BstDSI	CCRYGG	1 cut(s)	1189
BstEII	GGTNACC	1 cut(s)	983
BstF5I	GGATG	6 cut(s)	73, 1798, 1984, 2194, 2512, 2566
BstFNI	CGCG	1 cut(s)	839
BstH2I	RGCGCY	1 cut(s)	471
BstHHI	GCGC	3 cut(s)	470, 841, 2061
BstMAI	GTCTC	4 cut(s)	20, 439, 1211, 1920
BstMWI	GCNNNNNNNGC	7 cut(s)	335, 836, 1016, 1543, 1652, 1706, 2281
BstNI	CCWGG	3 cut(s)	1130, 1242, 1395
BstNSI	RCATGY	2 cut(s)	2387, 2654
BstPI	GGTNACC	1 cut(s)	983
BstSCI	CCNGG	4 cut(s)	659, 1128, 1240, 1393
BstSFI	CTRYAG	2 cut(s)	923, 2122
BstUI	CGCG	1 cut(s)	839
BstV1I	GCAGC	3 cut(s)	48, 58, 1028
BstX2I	RGATCY	6 cut(s)	83, 637, 997, 1526, 1570, 2592
BstXI	CCANNNNNNTGG	2 cut(s)	425, 2875
BstYI	RGATCY	6 cut(s)	83, 637, 997, 1526, 1570, 2592
Bsu15I	ATCGAT	1 cut(s)	974
BsuRI	GGCC	4 cut(s)	55, 1447, 1546, 1668
BsuTUI	ATCGAT	1 cut(s)	974
BtgI	CCRYGG	1 cut(s)	1189
BtrI	CACGTC	1 cut(s)	2394
BtsCI	GGATG	6 cut(s)	73, 1798, 1984, 2194, 2512, 2566
BtsIMutI	CAGTG	5 cut(s)	89, 478, 1554, 2080, 2582
Cac8I	GCNNGC	2 cut(s)	843, 2256
CaiI	CAGNNNCTG	1 cut(s)	3035
CciI	TCATGA	1 cut(s)	2388
CfoI	GCGC	3 cut(s)	470, 841, 2061
Cfr13I	GGNCC	6 cut(s)	54, 477, 520, 1445, 1544, 2857
ClaI	ATCGAT	1 cut(s)	974
CseI	GACGC	2 cut(s)	845, 1635
CsiI	ACCWGGT	1 cut(s)	1240
Csp6I	GTAC	6 cut(s)	392, 1073, 1266, 2119, 2399, 2717
CspCI	CAANNNNNGTGG	2 cut(s)	1765, 1800
CviQI	GTAC	6 cut(s)	392, 1073, 1266, 2119, 2399, 2717
DraI	TTTAAA	1 cut(s)	276
DraIII	CACNNNGTG	1 cut(s)	1244
Eam1104I	CTCTTC	2 cut(s)	516, 1213
EarI	CTCTTC	2 cut(s)	516, 1213
EciI	GGCGGA	1 cut(s)	404
Ecl136II	GAGCTC	1 cut(s)	2794
Eco130I	CCWWGG	2 cut(s)	1760, 2485
Eco24I	GRGCYC	1 cut(s)	2796
Eco31I	GGTCTC	2 cut(s)	20, 1920
Eco47I	GGWCC	3 cut(s)	477, 520, 2857
Eco53kI	GAGCTC	1 cut(s)	2794
Eco57I	CTGAAG	6 cut(s)	180, 497, 1103, 1272, 1941, 2978
Eco72I	CACGTG	1 cut(s)	2168
Eco91I	GGTNACC	1 cut(s)	983
EcoICRI	GAGCTC	1 cut(s)	2794
EcoO109I	RGGNCCY	2 cut(s)	477, 520
EcoO65I	GGTNACC	1 cut(s)	983
EcoRII	CCWGG	3 cut(s)	1128, 1240, 1393
EcoT14I	CCWWGG	2 cut(s)	1760, 2485
EcoT22I	ATGCAT	3 cut(s)	973, 2447, 2743
EcoT38I	GRGCYC	1 cut(s)	2796
ErhI	CCWWGG	2 cut(s)	1760, 2485
FalI	AAGNNNNNCTT	2 cut(s)	2145, 2177
FauI	CCCGC	2 cut(s)	2781, 2907
FauNDI	CATATG	1 cut(s)	1615
FblI	GTMKAC	1 cut(s)	1091
Fnu4HI	GCNGC	4 cut(s)	37, 72, 374, 1017
FokI	GGATG	6 cut(s)	80, 1785, 1991, 2201, 2499, 2573
FriOI	GRGCYC	1 cut(s)	2796
Fsp4HI	GCNGC	4 cut(s)	37, 72, 374, 1017
FspBI	CTAG	5 cut(s)	431, 534, 1986, 2019, 2114
GlaI	GCGC	3 cut(s)	469, 840, 2060
GluI	GCNGC	4 cut(s)	37, 72, 374, 1017
GsaI	CCCAGC	4 cut(s)	329, 2058, 2274, 2650
GsuI	CTGGAG	3 cut(s)	232, 1538, 2786
HaeII	RGCGCY	1 cut(s)	471
HaeIII	GGCC	4 cut(s)	55, 1447, 1546, 1668
HapII	CCGG	2 cut(s)	661, 3009
HgaI	GACGC	2 cut(s)	845, 1635
HhaI	GCGC	3 cut(s)	470, 841, 2061
Hin1I	GRCGYC	1 cut(s)	1646
Hin6I	GCGC	3 cut(s)	468, 839, 2059
HinP1I	GCGC	3 cut(s)	468, 839, 2059
HincII	GTYRAC	2 cut(s)	239, 769
HindII	GTYRAC	2 cut(s)	239, 769
HindIII	AAGCTT	1 cut(s)	262
HpaII	CCGG	2 cut(s)	661, 3009
HphI	GGTGA	7 cut(s)	741, 995, 1230, 1517, 2686, 2786, 3023
Hpy166II	GTNNAC	4 cut(s)	239, 769, 1092, 2399
Hpy8I	GTNNAC	4 cut(s)	239, 769, 1092, 2399
HpyCH4III	ACNGT	3 cut(s)	391, 604, 1827
HpyCH4IV	ACGT	2 cut(s)	2167, 2393
HpyF10VI	GCNNNNNNNGC	7 cut(s)	335, 836, 1016, 1543, 1652, 1706, 2281
HpySE526I	ACGT	2 cut(s)	2167, 2393
Hsp92I	GRCGYC	1 cut(s)	1646
HspAI	GCGC	3 cut(s)	468, 839, 2059
LmnI	GCTCC	6 cut(s)	193, 1557, 1973, 2251, 2548, 2791
Lsp1109I	GCAGC	3 cut(s)	48, 58, 1028
LweI	GCATC	8 cut(s)	58, 493, 520, 980, 1423, 2150, 2750, 2941
MabI	ACCWGGT	1 cut(s)	1240
MaeI	CTAG	5 cut(s)	431, 534, 1986, 2019, 2114
MaeII	ACGT	2 cut(s)	2167, 2393
MaeIII	GTNAC	6 cut(s)	910, 983, 1246, 1637, 1819, 2641
MbiI	CCGCTC	1 cut(s)	2788
MfeI	CAATTG	1 cut(s)	723
MflI	RGATCY	6 cut(s)	83, 637, 997, 1526, 1570, 2592
MhlI	GDGCHC	4 cut(s)	190, 898, 1554, 2796
MlyI	GAGTC	6 cut(s)	122, 637, 658, 2101, 2242, 2734
MmeI	TCCRAC	3 cut(s)	975, 1584, 1877
Mph1103I	ATGCAT	3 cut(s)	973, 2447, 2743
MroXI	GAANNNNTTC	1 cut(s)	2445
MseI	TTAA	7 cut(s)	144, 275, 978, 1688, 1695, 2180, 2288
MslI	CAYNNNNRTG	6 cut(s)	423, 906, 1503, 2517, 2736, 2949
MspA1I	CMGCKG	1 cut(s)	2914
MspCI	CTTAAG	1 cut(s)	2287
MspI	CCGG	2 cut(s)	661, 3009
MspR9I	CCNGG	4 cut(s)	661, 1130, 1242, 1395
MunI	CAATTG	1 cut(s)	723
Mva1269I	GAATGC	4 cut(s)	370, 1179, 2445, 2447
MvaI	CCWGG	3 cut(s)	1130, 1242, 1395
MvnI	CGCG	1 cut(s)	839
MwoI	GCNNNNNNNGC	7 cut(s)	335, 836, 1016, 1543, 1652, 1706, 2281
NciI	CCSGG	1 cut(s)	661
NdeI	CATATG	1 cut(s)	1615
NmuCI	GTSAC	4 cut(s)	983, 1246, 1637, 1819
NsiI	ATGCAT	3 cut(s)	973, 2447, 2743
NspI	RCATGY	2 cut(s)	2387, 2654
PagI	TCATGA	1 cut(s)	2388
PciI	ACATGT	1 cut(s)	2383
PcsI	WCGNNNNNNNCGW	1 cut(s)	1325
PctI	GAATGC	4 cut(s)	370, 1179, 2445, 2447
PdmI	GAANNNNTTC	1 cut(s)	2445
PfeI	GAWTC	8 cut(s)	400, 935, 1156, 1492, 1610, 1885, 2158, 2600
PkrI	GCNGC	4 cut(s)	38, 73, 375, 1018
PleI	GAGTC	6 cut(s)	121, 636, 658, 2100, 2241, 2733
PmaCI	CACGTG	1 cut(s)	2168
PmlI	CACGTG	1 cut(s)	2168
PpsI	GAGTC	6 cut(s)	121, 636, 658, 2100, 2241, 2733
Ppu21I	YACGTR	1 cut(s)	2168
PpuMI	RGGWCCY	2 cut(s)	477, 520
PscI	ACATGT	1 cut(s)	2383
PshBI	ATTAAT	2 cut(s)	978, 2180
PsiI	TTATAA	2 cut(s)	621, 2936
Psp124BI	GAGCTC	1 cut(s)	2796
Psp5II	RGGWCCY	2 cut(s)	477, 520
Psp6I	CCWGG	3 cut(s)	1128, 1240, 1393
PspCI	CACGTG	1 cut(s)	2168
PspEI	GGTNACC	1 cut(s)	983
PspFI	CCCAGC	4 cut(s)	325, 2054, 2270, 2646
PspGI	CCWGG	3 cut(s)	1128, 1240, 1393
PspPI	GGNCC	6 cut(s)	54, 477, 520, 1445, 1544, 2857
PspPPI	RGGWCCY	2 cut(s)	477, 520
PsrI	GAACNNNNNNTAC	2 cut(s)	190, 222
PstI	CTGCAG	1 cut(s)	2126
PstNI	CAGNNNCTG	1 cut(s)	3035
PsuI	RGATCY	6 cut(s)	83, 637, 997, 1526, 1570, 2592
RsaI	GTAC	6 cut(s)	393, 1074, 1267, 2120, 2400, 2718
RsaNI	GTAC	6 cut(s)	392, 1073, 1266, 2119, 2399, 2717
RseI	CAYNNNNRTG	6 cut(s)	423, 906, 1503, 2517, 2736, 2949
SacI	GAGCTC	1 cut(s)	2796
SaqAI	TTAA	7 cut(s)	144, 275, 978, 1688, 1695, 2180, 2288
SatI	GCNGC	4 cut(s)	37, 72, 374, 1017
Sau96I	GGNCC	6 cut(s)	54, 477, 520, 1445, 1544, 2857
ScaI	AGTACT	1 cut(s)	2120
SchI	GAGTC	6 cut(s)	122, 637, 658, 2101, 2242, 2734
ScrFI	CCNGG	4 cut(s)	661, 1130, 1242, 1395
SduI	GDGCHC	4 cut(s)	190, 898, 1554, 2796
SexAI	ACCWGGT	1 cut(s)	1240
SfaNI	GCATC	8 cut(s)	58, 493, 520, 980, 1423, 2150, 2750, 2941
SfcI	CTRYAG	2 cut(s)	923, 2122
SinI	GGWCC	3 cut(s)	477, 520, 2857
SmiMI	CAYNNNNRTG	6 cut(s)	423, 906, 1503, 2517, 2736, 2949
SmlI	CTYRAG	3 cut(s)	492, 2287, 2619
SmoI	CTYRAG	3 cut(s)	492, 2287, 2619
SsiI	CCGC	4 cut(s)	374, 415, 2788, 2914
SspI	AATATT	1 cut(s)	1857
SspMI	CTAG	5 cut(s)	431, 534, 1986, 2019, 2114
SstI	GAGCTC	1 cut(s)	2796
StyD4I	CCNGG	4 cut(s)	659, 1128, 1240, 1393
StyI	CCWWGG	2 cut(s)	1760, 2485
TaaI	ACNGT	3 cut(s)	391, 604, 1827
TaiI	ACGT	2 cut(s)	2170, 2396
TaqI	TCGA	6 cut(s)	175, 974, 1462, 2079, 2095, 2331
TatI	WGTACW	1 cut(s)	2118
TauI	GCSGC	1 cut(s)	376
TfiI	GAWTC	8 cut(s)	400, 935, 1156, 1492, 1610, 1885, 2158, 2600
Tru1I	TTAA	7 cut(s)	144, 275, 978, 1688, 1695, 2180, 2288
Tru9I	TTAA	7 cut(s)	144, 275, 978, 1688, 1695, 2180, 2288
TscAI	CASTG	5 cut(s)	96, 478, 1554, 2080, 2582
TseFI	GTSAC	4 cut(s)	983, 1246, 1637, 1819
TseI	GCWGC	3 cut(s)	36, 71, 1016
Tsp45I	GTSAC	4 cut(s)	983, 1246, 1637, 1819
TspGWI	ACGGA	1 cut(s)	2201
TspRI	CASTG	5 cut(s)	96, 478, 1554, 2080, 2582
Vha464I	CTTAAG	1 cut(s)	2287
VpaK11BI	GGWCC	3 cut(s)	477, 520, 2857
VspI	ATTAAT	2 cut(s)	978, 2180
XapI	RAATTY	5 cut(s)	739, 793, 1202, 1718, 2033
XceI	RCATGY	2 cut(s)	2387, 2654
XmiI	GTMKAC	1 cut(s)	1091
XmnI	GAANNNNTTC	1 cut(s)	2445
XspI	CTAG	5 cut(s)	431, 534, 1986, 2019, 2114
ZrmI	AGTACT	1 cut(s)	2120
Zsp2I	ATGCAT	3 cut(s)	973, 2447, 2743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.