AT3G20260

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011436
Physical Location & Seq
Reverse (-)
7063910 .. 7066729
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20260.1

Sequence Viewer

Length: 1314 bp
ATGGATTTCTTGAAAGTTAGAAAATTTCGAAAGTCTCGGAAGCCAAACCTTGAAAAGGAGAAAGAAGACAAGATATTGGCTCAGCAAGAACAGGCAAGGAGTGAGAACACTGAAGCTGGTGTGATAGATTCGGGGAAAGGAGATGAGATCGAAGATGACGATGATGATTTCATTACCAATGAGGTTAAGAGAAGGCTCAAGGAGTTGAGGAGAAACAGTTTCATGGTGTTGATACCTGAGGAAGAAGAAGAAGAAGAAGAGGAATCGTATCTAGATGAAGATGATGATGACGGAGAAGACAAGTGTTCAAGTGAGTGGAGAGATGTGGTTGCTGAAGGACTTCAATGGTGGGGAGGTTTTGATGCTGTCTATGAAAAGTATTGCGAGCGTATGCTCTTCTTTGATCGTTTAAGCTCTCAGCAGCTCAAGGAAACTGGCATTGGAATTGCTCCAAGTCCTTCAACTCCATCGCCAAGATCTGCATCTAAGAAGCTGTCATCGCCTTTCCGATGTCTTTCTCTCAAAAAGTTTGATGTTCCGGAGGAAGATATCGAGCATTTGCAGCCGACAGAAGTTGACGACCCTTATCAAGATCTAGAGACGGCCTATGTTGCTCAACTCTGCCTCACTTGGGAGGCACTTCACTGTCAATACACGCAGCTGAGCCACTTAATCTCATGCCAACCCGAAACCCCGACTTGCTACAACCATACTGCCCAACTGTTTCAGCAATTCCTAGTCTTGTTGCAGAGGTATATAGAGAATGAACCGTTTGAGCAAGGCTCAAGATCGGAACTTTATGCTCGTGCTAGAAATGCAATGCCTAAGCTACTTCAAGCTCCTAAGATTCAAGGGTCAGATAAAAAGGAGATGGAGAAAGATACAGGCTTCATGGTCCTCGCTGATGATCTCATCAAAGTCATAGAGAGCTCGATCCTTACTTTCAACGTCTTCTTGAAAATGGATAAGAAGAAACCAAATGGGGGTATTCACTTGTTTGGGAACCACAACAACAACCATGTGAATTCTACAACTCCTTTACTACTGGTTCAGTCATCCATTGATAAGAAAAGGGTGAAAGCAAAGGAGCTTTCAAAGAAGACAAAAGGGCTGAGAAAGAAATCATGGCCTCAAACATGGGAAGGTGTTCAACTCTTGTTTGCAGCCATTGACATCAAACTCGCAACACGGGTTTTGAGGATGTCGAAAATCAGTAAAGAGCAACTTCTATGGTGTGAAGAAAAGATGAAAAAGCTCAACTTTTCAGCTGGGAAGCTTCAAAGACACCCATCTCCAATTCTTTTCCCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

437

Amino Acids

50.51

Weight (kDa)

5.55

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 197 - 436 1.9e-92 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 538
AclWI GGATC 1 cut(s) 928
AcsI RAATTY 2 cut(s) 23, 1024
AcuI CTGAAG 2 cut(s) 132, 354
AfiI CCNNNNNNNGG 3 cut(s) 55, 631, 983
AjuI GAANNNNNNNTTGG 2 cut(s) 423, 455
Alw21I GWGCWC 1 cut(s) 932
Alw26I GTCTC 2 cut(s) 39, 593
AlwI GGATC 1 cut(s) 928
Aor13HI TCCGGA 1 cut(s) 538
AoxI GGCC 2 cut(s) 603, 1127
ApeKI GCWGC 4 cut(s) 421, 562, 658, 1163
ApoI RAATTY 2 cut(s) 23, 1024
Asp700I GAANNNNTTC 2 cut(s) 339, 1146
AspS9I GGNCC 1 cut(s) 895
AsuHPI GGTGA 1 cut(s) 1087
AsuII TTCGAA 1 cut(s) 28
AvaII GGWCC 1 cut(s) 895
AxyI CCTNAGG 1 cut(s) 237
BanII GRGCYC 1 cut(s) 932
BauI CACGAG 1 cut(s) 804
BbsI GAAGAC 4 cut(s) 72, 303, 943, 1106
Bbv12I GWGCWC 1 cut(s) 932
BbvI GCAGC 4 cut(s) 433, 574, 670, 1175
BccI CCATC 3 cut(s) 475, 865, 1297
BceAI ACGGC 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 39, 593
BfaI CTAG 4 cut(s) 272, 596, 737, 810
BglII AGATCT 2 cut(s) 476, 592
BisI GCNGC 4 cut(s) 422, 563, 659, 1164
BlpI GCTNAGC 2 cut(s) 81, 662
BlsI GCNGC 4 cut(s) 423, 564, 660, 1165
Bme18I GGWCC 1 cut(s) 895
BmgT120I GGNCC 1 cut(s) 895
BmiI GGNNCC 1 cut(s) 1004
BmsI GCATC 2 cut(s) 352, 491
BpiI GAAGAC 4 cut(s) 72, 303, 943, 1106
BplI GAGNNNNNCTC 2 cut(s) 767, 799
Bpu10I CCTNAGC 1 cut(s) 825
Bpu1102I GCTNAGC 2 cut(s) 81, 662
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 3 cut(s) 182, 410, 769
BsaBI GATNNNNATC 1 cut(s) 481
BsaWI WCCGGW 1 cut(s) 538
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 631, 983
Bse1I ACTGG 2 cut(s) 439, 1050
Bse21I CCTNAGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 825
Bse8I GATNNNNATC 1 cut(s) 481
BseAI TCCGGA 1 cut(s) 538
BseGI GGATG 2 cut(s) 1055, 1206
BseJI GATNNNNATC 1 cut(s) 481
BseLI CCNNNNNNNGG 3 cut(s) 55, 631, 983
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 5 cut(s) 95, 228, 431, 653, 1103
BseNI ACTGG 2 cut(s) 439, 1050
BseRI GAGGAG 1 cut(s) 223
BseXI GCAGC 4 cut(s) 433, 574, 670, 1175
BseYI CCCAGC 1 cut(s) 1268
BshFI GGCC 2 cut(s) 605, 1129
BsiHKAI GWGCWC 1 cut(s) 932
BsiSI CCGG 1 cut(s) 539
BslI CCNNNNNNNGG 3 cut(s) 55, 631, 983
BsmAI GTCTC 2 cut(s) 39, 593
BsmBI CGTCTC 1 cut(s) 593
BsnI GGCC 2 cut(s) 605, 1129
Bsp119I TTCGAA 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 932
Bsp13I TCCGGA 1 cut(s) 538
Bsp143I GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
Bsp1720I GCTNAGC 2 cut(s) 81, 662
BspANI GGCC 2 cut(s) 605, 1129
BspCNI CTCAG 5 cut(s) 94, 229, 430, 654, 1104
BspEI TCCGGA 1 cut(s) 538
BspLI GGNNCC 1 cut(s) 1004
BspPI GGATC 1 cut(s) 928
BspQI GCTCTTC 1 cut(s) 401
BspT104I TTCGAA 1 cut(s) 28
BsrDI GCAATG 1 cut(s) 825
BsrI ACTGG 2 cut(s) 439, 1050
BssMI GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
BssSI CACGAG 1 cut(s) 804
Bst2BI CACGAG 1 cut(s) 804
Bst4CI ACNGT 4 cut(s) 218, 647, 723, 771
Bst6I CTCTTC 2 cut(s) 252, 401
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 1 cut(s) 386
BstDEI CTNAG 8 cut(s) 81, 237, 417, 486, 662, 825, 843, 1112
BstENI CCTNNNNNAGG 1 cut(s) 53
BstF5I GGATG 2 cut(s) 1055, 1206
BstKTI GATC 7 cut(s) 150, 406, 479, 595, 791, 910, 936
BstMAI GTCTC 2 cut(s) 39, 593
BstMBI GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
BstMWI GCNNNNNNNGC 4 cut(s) 499, 562, 611, 815
BstV1I GCAGC 4 cut(s) 433, 574, 670, 1175
BstV2I GAAGAC 4 cut(s) 72, 303, 943, 1106
BstX2I RGATCY 2 cut(s) 476, 592
BstYI RGATCY 2 cut(s) 476, 592
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 2 cut(s) 605, 1129
BtgZI GCGATG 2 cut(s) 453, 483
BtsCI GGATG 2 cut(s) 1055, 1206
BtsIMutI CAGTG 2 cut(s) 108, 643
Cac8I GCNNGC 1 cut(s) 386
Cfr13I GGNCC 1 cut(s) 895
CviAII CATG 6 cut(s) 223, 678, 892, 1019, 1125, 1137
DdeI CTNAG 8 cut(s) 81, 237, 417, 486, 662, 825, 843, 1112
DpnI GATC 7 cut(s) 149, 405, 478, 594, 790, 909, 935
DpnII GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
Eam1104I CTCTTC 2 cut(s) 252, 401
EarI CTCTTC 2 cut(s) 252, 401
Ecl136II GAGCTC 1 cut(s) 930
Eco24I GRGCYC 1 cut(s) 932
Eco32I GATATC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 895
Eco53kI GAGCTC 1 cut(s) 930
Eco57I CTGAAG 2 cut(s) 132, 354
Eco81I CCTNAGG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 930
EcoNI CCTNNNNNAGG 1 cut(s) 53
EcoRI GAATTC 1 cut(s) 1024
EcoRV GATATC 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 932
Esp3I CGTCTC 1 cut(s) 593
FaeI CATG 6 cut(s) 226, 681, 895, 1022, 1128, 1140
FalI AAGNNNNNCTT 4 cut(s) 1209, 1241, 1244, 1276
FatI CATG 6 cut(s) 222, 677, 891, 1018, 1124, 1136
Fnu4HI GCNGC 4 cut(s) 422, 563, 659, 1164
FokI GGATG 2 cut(s) 1042, 1213
FriOI GRGCYC 1 cut(s) 932
Fsp4HI GCNGC 4 cut(s) 422, 563, 659, 1164
FspBI CTAG 4 cut(s) 272, 596, 737, 810
GluI GCNGC 4 cut(s) 422, 563, 659, 1164
GsaI CCCAGC 1 cut(s) 1272
HaeIII GGCC 2 cut(s) 605, 1129
HapII CCGG 1 cut(s) 539
Hin1II CATG 6 cut(s) 226, 681, 895, 1022, 1128, 1140
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 1274
HinfI GANTC 3 cut(s) 128, 263, 847
HpaII CCGG 1 cut(s) 539
HphI GGTGA 1 cut(s) 1087
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 4 cut(s) 39, 509, 793, 859
Hpy188III TCNNGA 7 cut(s) 10, 272, 539, 590, 596, 786, 955
Hpy8I GTNNAC 1 cut(s) 577
HpyAV CCTTC 4 cut(s) 186, 329, 468, 1136
HpyCH4III ACNGT 4 cut(s) 218, 647, 723, 771
HpyCH4IV ACGT 1 cut(s) 948
HpyCH4V TGCA 5 cut(s) 482, 562, 748, 818, 1163
HpyF10VI GCNNNNNNNGC 4 cut(s) 499, 562, 611, 815
HpyF3I CTNAG 8 cut(s) 81, 237, 417, 486, 662, 825, 843, 1112
HpySE526I ACGT 1 cut(s) 948
Hsp92II CATG 6 cut(s) 226, 681, 895, 1022, 1128, 1140
Kpn2I TCCGGA 1 cut(s) 538
Kzo9I GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 3 cut(s) 454, 844, 1087
LpnPI CCDG 8 cut(s) 77, 102, 249, 420, 552, 870, 1031, 1254
Lsp1109I GCAGC 4 cut(s) 433, 574, 670, 1175
LweI GCATC 2 cut(s) 352, 491
MaeI CTAG 4 cut(s) 272, 596, 737, 810
MaeII ACGT 1 cut(s) 948
MalI GATC 7 cut(s) 149, 405, 478, 594, 790, 909, 935
MboI GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
MflI RGATCY 2 cut(s) 476, 592
MhlI GDGCHC 1 cut(s) 932
MluCI AATT 5 cut(s) 23, 444, 731, 1024, 1296
MroI TCCGGA 1 cut(s) 538
MroXI GAANNNNTTC 2 cut(s) 339, 1146
MseI TTAA 3 cut(s) 186, 410, 671
MspA1I CMGCKG 2 cut(s) 661, 1268
MspI CCGG 1 cut(s) 539
MwoI GCNNNNNNNGC 4 cut(s) 499, 562, 611, 815
NdeII GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
NlaIII CATG 6 cut(s) 226, 681, 895, 1022, 1128, 1140
NlaIV GGNNCC 1 cut(s) 1004
NspV TTCGAA 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 339, 1146
PfeI GAWTC 3 cut(s) 128, 263, 847
PkrI GCNGC 4 cut(s) 423, 564, 660, 1165
Psp124BI GAGCTC 1 cut(s) 932
PspFI CCCAGC 1 cut(s) 1268
PspN4I GGNNCC 1 cut(s) 1004
PspPI GGNCC 1 cut(s) 895
PsuI RGATCY 2 cut(s) 476, 592
PvuII CAGCTG 2 cut(s) 661, 1268
SacI GAGCTC 1 cut(s) 932
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 3 cut(s) 186, 410, 671
SatI GCNGC 4 cut(s) 422, 563, 659, 1164
Sau3AI GATC 7 cut(s) 147, 403, 476, 592, 788, 907, 933
Sau96I GGNCC 1 cut(s) 895
SduI GDGCHC 1 cut(s) 932
SfaNI GCATC 2 cut(s) 352, 491
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 1 cut(s) 895
SmlI CTYRAG 3 cut(s) 197, 425, 784
SmoI CTYRAG 3 cut(s) 197, 425, 784
Sse9I AATT 5 cut(s) 23, 444, 731, 1024, 1296
SspMI CTAG 4 cut(s) 272, 596, 737, 810
SstI GAGCTC 1 cut(s) 932
TaaI ACNGT 4 cut(s) 218, 647, 723, 771
TaiI ACGT 1 cut(s) 951
TaqI TCGA 5 cut(s) 28, 150, 552, 932, 1205
TasI AATT 5 cut(s) 23, 444, 731, 1024, 1296
TfiI GAWTC 3 cut(s) 128, 263, 847
Tru1I TTAA 3 cut(s) 186, 410, 671
Tru9I TTAA 3 cut(s) 186, 410, 671
TscAI CASTG 2 cut(s) 115, 650
TseI GCWGC 4 cut(s) 421, 562, 658, 1163
TspDTI ATGAA 7 cut(s) 160, 211, 291, 387, 780, 880, 1262
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 2 cut(s) 115, 650
VpaK11BI GGWCC 1 cut(s) 895
XagI CCTNNNNNAGG 1 cut(s) 53
XapI RAATTY 2 cut(s) 23, 1024
XbaI TCTAGA 2 cut(s) 271, 595
XmnI GAANNNNTTC 2 cut(s) 339, 1146
XspI CTAG 4 cut(s) 272, 596, 737, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.