Prupe.3G023200_v2.0.a1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0011436
Physical Location & Seq
Forward (+)
1711392 .. 1715823
4432 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G023200.1

Sequence Viewer

Length: 1284 bp
ATGGATTTCTTCAAAGTTAAAAAGTTTCGAAAAGCCCACAAACCAGAACCAGAAAATGTTTCAGAGGACAATCCTGTGCCACAGCCGGAGGAACCTAGGAAGGAGAGTGGTGATGATTTTGGTAAGTCAGCTAATGCTGATTCTATAGCTGAGGCCGAGGATGATGATGATGATTTCATAACAGAGGAGGTCAAGCGGAGGTTAAAAGAATTGAGAAGGAATAGTTTTATGGTGTTGATTCCGGAAGAAGAGTCATTCCCGGAAGAGGATGAAGAAGAAGAGCATGGTGAGACAAGCTCTAACGAGTGGAGGGATGTAGAAGCAGAAGGCCGGCAATGGTGGTGTGGCTTTGGTGCTGTATATGACAAGTACTGCGAGAGAATGTTGTTCTTTGAACGGATGAGTGCGCAGCATCTTAATGAAACTGGTATCCAAACCTCTACAACTCCATCACCAAGATCTGCATCTAAGAAGCTTACCTCCCCTTTTCGCTGCCTTTCTTTGAAGAAGATTGAAGAACCTGATGATGAGACTGAGCATCTTCAGCAGCCAGAGAATGATCCTTATCAGGATATTGAGACATCATATGTAGCTCAAATTTGCTTGTCTTGGGAGGCACTGCACTGTCAGTACACTCAACTGAACCAGCTAATCACATGCCAACCTGACAATCCTAACTGTTATAACCACAGTGCTCAACAGTTTCAACAATTCCAGGTTTTATTACAAAGGTTTATTGAAAATGAACCTTTTGAGGAGGGCCACAGGGCTGAAATTTATGCTCGCACGCAGAGACATCTGCCTAAATTACTCCGGGTCCCCGATATACAAAGTCCAGTTAAAAAAGGGACAGAGGAAGAAGAATCAGATTATATGGTTCATGCCCCTGATCTCATCAAGATTATTGAAACCTCAATCCTCACTTTTCAGCTTTTCGTGAAGATGGATAAGAAAAAAACAAGCTCCGTTCTTAACTTGTTTGGGAATCAAAACCAGGCTGCCACCCCACTTCAGCAGATTCAGTCTTCTCTTGAGAAGAAATGGATGAAACTAAAGGAACTGCGGAACAAGAGGAAAGGCTGGAAGAAGAAATCCTGGCCACAATCACAAGAAGATGTTCAGCTTTTGTTTGGCCTCATTGATGCCAAAGTTTTGTCGAGGGTTCTTCGGATGGTGAGAATAAGCAAAGAGCAGCTGTTTTGGTGTGAGGAAAAGATGAAAAAACTAGATTTAGTAGACGGCAAGTTGTGGAGAGACCCCTCTCCCACCCTTTTCCCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

428

Amino Acids

50.09

Weight (kDa)

5.2

Isoelectric Point (pI)

63.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
Acc16I TGCGCA 1 cut(s) 408
AccI GTMKAC 1 cut(s) 1236
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 2 cut(s) 196, 1063
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 1 cut(s) 1097
AcsI RAATTY 2 cut(s) 597, 774
AcuI CTGAAG 2 cut(s) 527, 995
AfaI GTAC 2 cut(s) 371, 632
AfiI CCNNNNNNNGG 1 cut(s) 265
AgsI TTSAA 7 cut(s) 13, 395, 505, 515, 707, 740, 908
AjnI CCWGG 3 cut(s) 714, 993, 1094
Alw21I GWGCWC 1 cut(s) 697
Alw26I GTCTC 5 cut(s) 284, 524, 572, 787, 1248
AlwI GGATC 1 cut(s) 554
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 5 cut(s) 153, 328, 760, 1097, 1132
ApeKI GCWGC 5 cut(s) 409, 492, 547, 998, 1192
ApoI RAATTY 2 cut(s) 597, 774
Asp700I GAANNNNTTC 1 cut(s) 1116
AspA2I CCTAGG 1 cut(s) 95
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 2 cut(s) 760, 817
AsuC2I CCSGG 2 cut(s) 260, 815
AsuHPI GGTGA 4 cut(s) 122, 299, 444, 1186
AsuII TTCGAA 1 cut(s) 28
AvaII GGWCC 1 cut(s) 817
AvrII CCTAGG 1 cut(s) 95
BalI TGGCCA 1 cut(s) 1099
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 697
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 5 cut(s) 421, 479, 559, 985, 1204
BccI CCATC 3 cut(s) 457, 937, 1165
BceAI ACGGC 1 cut(s) 1255
BciT130I CCWGG 3 cut(s) 716, 995, 1096
BciVI GTATCC 1 cut(s) 440
BcnI CCSGG 2 cut(s) 260, 815
BcoDI GTCTC 5 cut(s) 284, 524, 572, 787, 1248
BfaI CTAG 2 cut(s) 96, 1226
BfmI CTRYAG 1 cut(s) 144
BfuI GTATCC 1 cut(s) 440
BglII AGATCT 1 cut(s) 458
BisI GCNGC 5 cut(s) 410, 493, 548, 999, 1193
BlnI CCTAGG 1 cut(s) 95
BlsI GCNGC 5 cut(s) 411, 494, 549, 1000, 1194
BmcAI AGTACT 1 cut(s) 371
Bme1390I CCNGG 5 cut(s) 260, 716, 815, 995, 1096
Bme18I GGWCC 1 cut(s) 817
BmgT120I GGNCC 2 cut(s) 760, 817
BmiI GGNNCC 3 cut(s) 93, 818, 819
BmrFI CCNGG 5 cut(s) 260, 716, 815, 995, 1096
BmsI GCATC 4 cut(s) 421, 473, 547, 1132
BpiI GAAGAC 1 cut(s) 1017
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu10I CCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 1052
BpuMI CCSGG 2 cut(s) 260, 815
BsaBI GATNNNNATC 2 cut(s) 463, 564
BsaI GGTCTC 1 cut(s) 1248
BsaJI CCNNGG 2 cut(s) 95, 156
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 265
Bse118I RCCGGY 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 430, 836
Bse3DI GCAATG 1 cut(s) 341
Bse8I GATNNNNATC 2 cut(s) 463, 564
BseAI TCCGGA 1 cut(s) 241
BseBI CCWGG 3 cut(s) 716, 995, 1096
BseDI CCNNGG 2 cut(s) 95, 156
BseGI GGATG 6 cut(s) 166, 274, 319, 405, 1050, 1176
BseJI GATNNNNATC 2 cut(s) 463, 564
BseLI CCNNNNNNNGG 1 cut(s) 265
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 2 cut(s) 141, 525
BseNI ACTGG 2 cut(s) 430, 836
BseRI GAGGAG 2 cut(s) 200, 770
BseXI GCAGC 5 cut(s) 421, 479, 559, 985, 1204
BsgI GTGCAG 1 cut(s) 605
BshFI GGCC 5 cut(s) 155, 330, 762, 1099, 1134
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 5 cut(s) 86, 242, 260, 331, 814
BslFI GGGAC 2 cut(s) 803, 862
BslI CCNNNNNNNGG 1 cut(s) 265
BsmAI GTCTC 5 cut(s) 284, 524, 572, 787, 1248
BsmFI GGGAC 2 cut(s) 803, 862
BsnI GGCC 5 cut(s) 155, 330, 762, 1099, 1134
Bso31I GGTCTC 1 cut(s) 1248
Bsp119I TTCGAA 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 697
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 3 cut(s) 458, 559, 889
BspACI CCGC 2 cut(s) 196, 1063
BspANI GGCC 5 cut(s) 155, 330, 762, 1099, 1134
BspCNI CTCAG 2 cut(s) 142, 526
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 93, 818, 819
BspPI GGATC 1 cut(s) 554
BspQI GCTCTTC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 1248
BsrDI GCAATG 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 330
BsrI ACTGG 2 cut(s) 430, 836
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 2 cut(s) 95, 156
BssMI GATC 3 cut(s) 458, 559, 889
BssT1I CCWWGG 1 cut(s) 95
Bst2UI CCWGG 3 cut(s) 716, 995, 1096
Bst4CI ACNGT 4 cut(s) 626, 680, 692, 702
Bst6I CTCTTC 3 cut(s) 243, 258, 273
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 332, 784, 788
BstDEI CTNAG 3 cut(s) 150, 468, 534
BstF5I GGATG 6 cut(s) 166, 274, 319, 405, 1050, 1176
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 3 cut(s) 461, 562, 892
BstMAI GTCTC 5 cut(s) 284, 524, 572, 787, 1248
BstMBI GATC 3 cut(s) 458, 559, 889
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 3 cut(s) 716, 995, 1096
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 5 cut(s) 258, 714, 813, 993, 1094
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 5 cut(s) 421, 479, 559, 985, 1204
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 1 cut(s) 458
BstYI RGATCY 1 cut(s) 458
BsuI GTATCC 1 cut(s) 440
BsuRI GGCC 5 cut(s) 155, 330, 762, 1099, 1134
BtsCI GGATG 6 cut(s) 166, 274, 319, 405, 1050, 1176
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 3 cut(s) 617, 622, 697
Cac8I GCNNGC 3 cut(s) 332, 784, 788
CfoI GCGC 1 cut(s) 409
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 760, 817
Csp6I GTAC 2 cut(s) 370, 631
CviAII CATG 3 cut(s) 284, 657, 881
CviQI GTAC 2 cut(s) 370, 631
DdeI CTNAG 3 cut(s) 150, 468, 534
DpnI GATC 3 cut(s) 460, 561, 891
DpnII GATC 3 cut(s) 458, 559, 889
EaeI YGGCCR 1 cut(s) 1097
Eam1104I CTCTTC 3 cut(s) 243, 258, 273
EarI CTCTTC 3 cut(s) 243, 258, 273
Eco130I CCWWGG 1 cut(s) 95
Eco31I GGTCTC 1 cut(s) 1248
Eco47I GGWCC 1 cut(s) 817
Eco57I CTGAAG 2 cut(s) 527, 995
EcoO109I RGGNCCY 1 cut(s) 817
EcoRII CCWGG 3 cut(s) 714, 993, 1094
EcoT14I CCWWGG 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 3 cut(s) 287, 660, 884
FaqI GGGAC 2 cut(s) 803, 862
FatI CATG 3 cut(s) 283, 656, 880
FauNDI CATATG 1 cut(s) 586
FblI GTMKAC 1 cut(s) 1236
Fnu4HI GCNGC 5 cut(s) 410, 493, 548, 999, 1193
FokI GGATG 6 cut(s) 173, 281, 326, 412, 1057, 1183
Fsp4HI GCNGC 5 cut(s) 410, 493, 548, 999, 1193
FspBI CTAG 2 cut(s) 96, 1226
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 1 cut(s) 408
GluI GCNGC 5 cut(s) 410, 493, 548, 999, 1193
HaeIII GGCC 5 cut(s) 155, 330, 762, 1099, 1134
HapII CCGG 5 cut(s) 86, 242, 260, 331, 814
HhaI GCGC 1 cut(s) 409
Hin1II CATG 3 cut(s) 287, 660, 884
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 6 cut(s) 140, 238, 251, 863, 985, 1018
HpaII CCGG 5 cut(s) 86, 242, 260, 331, 814
HphI GGTGA 4 cut(s) 122, 299, 444, 1186
Hpy166II GTNNAC 2 cut(s) 633, 1237
Hpy188I TCNGA 3 cut(s) 64, 868, 1170
Hpy188III TCNNGA 5 cut(s) 242, 569, 898, 937, 1031
Hpy8I GTNNAC 2 cut(s) 633, 1237
HpyAV CCTTC 3 cut(s) 94, 210, 320
HpyCH4III ACNGT 4 cut(s) 626, 680, 692, 702
HpyCH4V TGCA 2 cut(s) 464, 622
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 3 cut(s) 150, 468, 534
Hsp92II CATG 3 cut(s) 287, 660, 884
HspAI GCGC 1 cut(s) 407
KflI GGGWCCC 1 cut(s) 817
Kpn2I TCCGGA 1 cut(s) 241
KroI GCCGGC 1 cut(s) 330
KroNI GCCGGC 1 cut(s) 332
Kzo9I GATC 3 cut(s) 458, 559, 889
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 968
Lsp1109I GCAGC 5 cut(s) 421, 479, 559, 985, 1204
LweI GCATC 4 cut(s) 421, 473, 547, 1132
MaeI CTAG 2 cut(s) 96, 1226
MalI GATC 3 cut(s) 460, 561, 891
MboI GATC 3 cut(s) 458, 559, 889
MflI RGATCY 1 cut(s) 458
MhlI GDGCHC 1 cut(s) 697
MlsI TGGCCA 1 cut(s) 1099
MluCI AATT 5 cut(s) 209, 597, 710, 774, 806
MluNI TGGCCA 1 cut(s) 1099
MlyI GAGTC 1 cut(s) 260
Mox20I TGGCCA 1 cut(s) 1099
MroI TCCGGA 1 cut(s) 241
MroNI GCCGGC 1 cut(s) 330
MroXI GAANNNNTTC 1 cut(s) 1116
MscI TGGCCA 1 cut(s) 1099
MseI TTAA 6 cut(s) 18, 203, 417, 840, 972, 1282
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 1099
MspA1I CMGCKG 1 cut(s) 1195
MspI CCGG 5 cut(s) 86, 242, 260, 331, 814
MspR9I CCNGG 5 cut(s) 260, 716, 815, 995, 1096
MvaI CCWGG 3 cut(s) 716, 995, 1096
MwoI GCNNNNNNNGC 1 cut(s) 544
NaeI GCCGGC 1 cut(s) 332
NciI CCSGG 2 cut(s) 260, 815
NdeI CATATG 1 cut(s) 586
NdeII GATC 3 cut(s) 458, 559, 889
NgoMIV GCCGGC 1 cut(s) 330
NlaIII CATG 3 cut(s) 287, 660, 884
NlaIV GGNNCC 3 cut(s) 93, 818, 819
NmeAIII GCCGAG 1 cut(s) 181
NsbI TGCGCA 1 cut(s) 408
NspI RCATGY 1 cut(s) 660
NspV TTCGAA 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 1116
PfeI GAWTC 5 cut(s) 140, 238, 863, 985, 1018
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 5 cut(s) 411, 494, 549, 1000, 1194
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 817
PsiI TTATAA 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 714, 993, 1094
PspGI CCWGG 3 cut(s) 714, 993, 1094
PspN4I GGNNCC 3 cut(s) 93, 818, 819
PspPI GGNCC 2 cut(s) 760, 817
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 1 cut(s) 458
PvuII CAGCTG 1 cut(s) 1195
RsaI GTAC 2 cut(s) 371, 632
RsaNI GTAC 2 cut(s) 370, 631
RseI CAYNNNNRTG 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 6 cut(s) 18, 203, 417, 840, 972, 1282
SatI GCNGC 5 cut(s) 410, 493, 548, 999, 1193
Sau3AI GATC 3 cut(s) 458, 559, 889
Sau96I GGNCC 2 cut(s) 760, 817
ScaI AGTACT 1 cut(s) 371
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 5 cut(s) 260, 716, 815, 995, 1096
SduI GDGCHC 1 cut(s) 697
SfaNI GCATC 4 cut(s) 421, 473, 547, 1132
SfcI CTRYAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 1031
SmoI CTYRAG 1 cut(s) 1031
Sse9I AATT 5 cut(s) 209, 597, 710, 774, 806
SsiI CCGC 2 cut(s) 196, 1063
SspMI CTAG 2 cut(s) 96, 1226
StyD4I CCNGG 5 cut(s) 258, 714, 813, 993, 1094
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 4 cut(s) 626, 680, 692, 702
TaqI TCGA 2 cut(s) 28, 1157
TasI AATT 5 cut(s) 209, 597, 710, 774, 806
TatI WGTACW 2 cut(s) 369, 630
TfiI GAWTC 5 cut(s) 140, 238, 863, 985, 1018
Tru1I TTAA 6 cut(s) 18, 203, 417, 840, 972, 1282
Tru9I TTAA 6 cut(s) 18, 203, 417, 840, 972, 1282
TscAI CASTG 3 cut(s) 624, 629, 697
TseI GCWGC 5 cut(s) 409, 492, 547, 998, 1192
TspDTI ATGAA 7 cut(s) 166, 285, 435, 759, 869, 1061, 1232
TspGWI ACGGA 2 cut(s) 412, 955
TspRI CASTG 3 cut(s) 624, 629, 697
VpaK11BI GGWCC 1 cut(s) 817
XapI RAATTY 2 cut(s) 597, 774
XceI RCATGY 1 cut(s) 660
XmaJI CCTAGG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 1236
XmnI GAANNNNTTC 1 cut(s) 1116
XspI CTAG 2 cut(s) 96, 1226
ZrmI AGTACT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.