Rh2DG492800

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011436
Physical Location & Seq
Reverse (-)
71339527 .. 71344248
4722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG492800.1

Sequence Viewer

Length: 1278 bp
ATGGATTTCTTCAAAGTTAAGAAGTTTAGAAAAGCTCACAAACCAGACCCAGAAAATGAGTTGGAGGACAATCCTGTGCCTGTGCCAGAGGAACCGAAGGAGAATGGTGGTGATGAAAAAGGCAAGGCCGTTAATGTGGATCCTATAGCTGAAGCTGATGATGACGATGATGATTTCATAACAGAGGAAGTCAAGAGAAGGTTAAAAGAATTGCGAAGGAATAGTTTTATGGTATTGATCCCGGAAGAAGAGTCATGTGCTGAAGAGGAGGAGGAAGAAGAAGAGCAGGGAGAGACGAGCTCCAATGAGTGGAGGGATGTAGAAGCTGAGGGTCGGCAATGGTGGTGTGGCTTTGGTGCTGTATATGACAAATACTGTGAGAGGATGTTGTTCTTTGAACGGATGAGCACACAGCAGCTTAAGGAAGGCATCCAAACCCCTACAACTCCATCACCAAGACCTGCATCTAAGAAGCTTTCCTCTCCTTTTCGCTGTCTCTCTTTGAAGAAGATTGAAGAACCTAATGACGAGACTGAGCATCTCCAGCAGCCAGAGATTGACCCTTATCATGATATTGAGACATCATATGTAGCTCAAGTTTGCTTGTCTTGGGAGGCACTGCACTGTCAATACACTCAACTGAATCAGCTAATCTCATGCCAACCTGAAAATCCCACCCCCTACAACCACAGTGCTCAACAGTTTCAACAATTTCAGGTTTTATTACAAAGGTTTATTGAAAATGAACCTTTTGAGGAGGGTCTCAGGCCAGAAATTTATGCTCGCACGCAGAGAGTTCTGCCTAAACTACTCCGGGTCCCTAATATACAAGGTCCAATTAATAAAGGGAAAGAAGAAGAAGAATCAGATTCCTTGATTCTTGCCCCTGATCTGATCAAGATTATTGAAACCTCGATCCTCACATTTCTACTTTTCCTGAAGATGGACAAGAAAAAAACAAGTGCTGGTCTTAGCTTATTTGGAAATCAAAACCATGCTACCCCACTTCAGCAGATTCAAACTTCACTGGATAAGAAATGGATGAAATTAAAGGAACTTCGTAAAAAGAGGAAAAGTCGGAAGAAGTCATGGCCGCAATCACAAGAAGATGTTCAGCTGTTGTTTAGCCTCACTGATGCAAAGGTTTTGTCAAGGGTTCTCCGGATGGGAAGAATTAGTAAAGAGCAGCTATTTTGGTGCGAGGAAAAGATGAAAAAGCTAGATTTGGTGGATGGCAAGTTGTGGAGAGACCCCTCTCCCACCCTTTTCCCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

425

Amino Acids

49.61

Weight (kDa)

5.18

Isoelectric Point (pI)

62.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 190 - 424 3.9e-88 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 469
AccB7I CCANNNNNTGG 1 cut(s) 309
AccIII TCCGGA 1 cut(s) 1161
AciI CCGC 1 cut(s) 1094
AclWI GGATC 4 cut(s) 134, 147, 232, 910
AcoI YGGCCR 1 cut(s) 1091
AcsI RAATTY 1 cut(s) 774
AcuI CTGAAG 4 cut(s) 171, 282, 959, 992
AfiI CCNNNNNNNGG 2 cut(s) 309, 943
AflII CTTAAG 1 cut(s) 419
AgsI TTSAA 8 cut(s) 13, 398, 505, 515, 707, 740, 908, 1019
Alw21I GWGCWC 3 cut(s) 302, 410, 697
Alw26I GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
AlwI GGATC 4 cut(s) 134, 147, 232, 910
Aor13HI TCCGGA 1 cut(s) 1161
AoxI GGCC 3 cut(s) 126, 767, 1091
ApeKI GCWGC 3 cut(s) 415, 547, 1186
ApoI RAATTY 1 cut(s) 774
ArsI GACNNNNNNTTYG 2 cut(s) 1133, 1165
AseI ATTAAT 1 cut(s) 840
Asp700I GAANNNNTTC 1 cut(s) 1110
AspS9I GGNCC 2 cut(s) 817, 833
AsuC2I CCSGG 2 cut(s) 242, 815
AsuHPI GGTGA 2 cut(s) 122, 444
AvaII GGWCC 2 cut(s) 817, 833
BamHI GGATCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 302
Bbv12I GWGCWC 3 cut(s) 302, 410, 697
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 427, 559, 1198
BccI CCATC 4 cut(s) 457, 937, 1159, 1226
BceAI ACGGC 1 cut(s) 113
BclI TGATCA 1 cut(s) 894
BcnI CCSGG 2 cut(s) 242, 815
BcoDI GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
BfaI CTAG 1 cut(s) 1220
BfmI CTRYAG 1 cut(s) 144
BfrI CTTAAG 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 4 cut(s) 416, 548, 1094, 1187
BlsI GCNGC 4 cut(s) 417, 549, 1095, 1188
Bme1390I CCNGG 2 cut(s) 242, 815
Bme18I GGWCC 2 cut(s) 817, 833
BmgT120I GGNCC 2 cut(s) 817, 833
BmiI GGNNCC 4 cut(s) 93, 141, 818, 819
BmrFI CCNGG 2 cut(s) 242, 815
BmsI GCATC 4 cut(s) 438, 473, 547, 1126
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 579
BpuMI CCSGG 2 cut(s) 242, 815
BsaI GGTCTC 2 cut(s) 767, 1242
BsaWI WCCGGW 1 cut(s) 1161
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 943
Bse1I ACTGG 1 cut(s) 1032
Bse3DI GCAATG 1 cut(s) 344
BseAI TCCGGA 1 cut(s) 1161
BseGI GGATG 7 cut(s) 322, 390, 408, 429, 1047, 1170, 1237
BseLI CCNNNNNNNGG 2 cut(s) 309, 943
BseMI GCAATG 1 cut(s) 344
BseMII CTCAG 3 cut(s) 318, 525, 778
BseNI ACTGG 1 cut(s) 1032
BseRI GAGGAG 3 cut(s) 281, 284, 770
BseXI GCAGC 3 cut(s) 427, 559, 1198
BsgI GTGCAG 1 cut(s) 605
BshFI GGCC 3 cut(s) 128, 769, 1093
BsiHKAI GWGCWC 3 cut(s) 302, 410, 697
BsiSI CCGG 3 cut(s) 242, 814, 1162
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 2 cut(s) 309, 943
BsmAI GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 803
BsnI GGCC 3 cut(s) 128, 769, 1093
Bso31I GGTCTC 2 cut(s) 767, 1242
Bsp1286I GDGCHC 3 cut(s) 302, 410, 697
Bsp13I TCCGGA 1 cut(s) 1161
Bsp143I GATC 5 cut(s) 139, 237, 889, 894, 915
BspACI CCGC 1 cut(s) 1094
BspANI GGCC 3 cut(s) 128, 769, 1093
BspCNI CTCAG 3 cut(s) 319, 526, 777
BspEI TCCGGA 1 cut(s) 1161
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 4 cut(s) 93, 141, 818, 819
BspMI ACCTGC 1 cut(s) 469
BspPI GGATC 4 cut(s) 134, 147, 232, 910
BspQI GCTCTTC 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 419
BspTNI GGTCTC 2 cut(s) 767, 1242
BsrDI GCAATG 1 cut(s) 344
BsrI ACTGG 1 cut(s) 1032
BssMI GATC 5 cut(s) 139, 237, 889, 894, 915
Bst4CI ACNGT 4 cut(s) 377, 626, 692, 702
Bst6I CTCTTC 3 cut(s) 243, 258, 276
BstAFI CTTAAG 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 784, 788
BstDEI CTNAG 5 cut(s) 327, 468, 534, 764, 971
BstF5I GGATG 7 cut(s) 322, 390, 408, 429, 1047, 1170, 1237
BstKTI GATC 5 cut(s) 142, 240, 892, 897, 918
BstMAI GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
BstMBI GATC 5 cut(s) 139, 237, 889, 894, 915
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstSCI CCNGG 2 cut(s) 240, 813
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 427, 559, 1198
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 3 cut(s) 128, 769, 1093
BtsCI GGATG 7 cut(s) 322, 390, 408, 429, 1047, 1170, 1237
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 5 cut(s) 617, 622, 697, 1025, 1131
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 2 cut(s) 784, 788
CciI TCATGA 1 cut(s) 568
Cfr13I GGNCC 2 cut(s) 817, 833
CviAII CATG 5 cut(s) 255, 569, 657, 995, 1089
DdeI CTNAG 5 cut(s) 327, 468, 534, 764, 971
DpnI GATC 5 cut(s) 141, 239, 891, 896, 917
DpnII GATC 5 cut(s) 139, 237, 889, 894, 915
EaeI YGGCCR 1 cut(s) 1091
Eam1104I CTCTTC 3 cut(s) 243, 258, 276
EarI CTCTTC 3 cut(s) 243, 258, 276
Ecl136II GAGCTC 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 302
Eco31I GGTCTC 2 cut(s) 767, 1242
Eco47I GGWCC 2 cut(s) 817, 833
Eco53kI GAGCTC 1 cut(s) 300
Eco57I CTGAAG 4 cut(s) 171, 282, 959, 992
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 302
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 5 cut(s) 258, 572, 660, 998, 1092
FaqI GGGAC 1 cut(s) 803
FatI CATG 5 cut(s) 254, 568, 656, 994, 1088
FauNDI CATATG 1 cut(s) 586
FbaI TGATCA 1 cut(s) 894
Fnu4HI GCNGC 4 cut(s) 416, 548, 1094, 1187
FokI GGATG 7 cut(s) 329, 397, 415, 416, 1054, 1177, 1244
FriOI GRGCYC 1 cut(s) 302
Fsp4HI GCNGC 4 cut(s) 416, 548, 1094, 1187
FspBI CTAG 1 cut(s) 1220
GluI GCNGC 4 cut(s) 416, 548, 1094, 1187
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 3 cut(s) 128, 769, 1093
HapII CCGG 3 cut(s) 242, 814, 1162
Hin1II CATG 5 cut(s) 258, 572, 660, 998, 1092
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 6 cut(s) 251, 643, 863, 869, 877, 1015
HpaII CCGG 3 cut(s) 242, 814, 1162
HphI GGTGA 2 cut(s) 122, 444
Hpy188I TCNGA 3 cut(s) 868, 894, 1080
Hpy188III TCNNGA 5 cut(s) 193, 569, 898, 937, 1162
HpyAV CCTTC 4 cut(s) 91, 192, 210, 419
HpyCH4III ACNGT 4 cut(s) 377, 626, 692, 702
HpyCH4V TGCA 3 cut(s) 464, 622, 1139
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 5 cut(s) 327, 468, 534, 764, 971
Hsp92II CATG 5 cut(s) 258, 572, 660, 998, 1092
KflI GGGWCCC 1 cut(s) 817
Kpn2I TCCGGA 1 cut(s) 1161
Ksp22I TGATCA 1 cut(s) 894
Kzo9I GATC 5 cut(s) 139, 237, 889, 894, 915
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 305
Lsp1109I GCAGC 3 cut(s) 427, 559, 1198
LweI GCATC 4 cut(s) 438, 473, 547, 1126
MaeI CTAG 1 cut(s) 1220
MalI GATC 5 cut(s) 141, 239, 891, 896, 917
MboI GATC 5 cut(s) 139, 237, 889, 894, 915
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 3 cut(s) 302, 410, 697
MluCI AATT 6 cut(s) 209, 710, 774, 837, 1046, 1173
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 42, 1058
MroI TCCGGA 1 cut(s) 1161
MroXI GAANNNNTTC 1 cut(s) 1110
MseI TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
MspA1I CMGCKG 1 cut(s) 1117
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 3 cut(s) 242, 814, 1162
MspR9I CCNGG 2 cut(s) 242, 815
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 2 cut(s) 242, 815
NdeI CATATG 1 cut(s) 586
NdeII GATC 5 cut(s) 139, 237, 889, 894, 915
NlaIII CATG 5 cut(s) 258, 572, 660, 998, 1092
NlaIV GGNNCC 4 cut(s) 93, 141, 818, 819
PagI TCATGA 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 5 cut(s) 643, 863, 869, 877, 1015
PflMI CCANNNNNTGG 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 4 cut(s) 417, 549, 1095, 1188
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
PpuMI RGGWCCY 1 cut(s) 817
PshBI ATTAAT 1 cut(s) 840
Psp124BI GAGCTC 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 817
PspN4I GGNNCC 4 cut(s) 93, 141, 818, 819
PspPI GGNCC 2 cut(s) 817, 833
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 1117
SacI GAGCTC 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
SatI GCNGC 4 cut(s) 416, 548, 1094, 1187
Sau3AI GATC 5 cut(s) 139, 237, 889, 894, 915
Sau96I GGNCC 2 cut(s) 817, 833
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 242, 815
SduI GDGCHC 3 cut(s) 302, 410, 697
SfaNI GCATC 4 cut(s) 438, 473, 547, 1126
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 2 cut(s) 817, 833
SmlI CTYRAG 2 cut(s) 419, 594
SmoI CTYRAG 2 cut(s) 419, 594
Sse9I AATT 6 cut(s) 209, 710, 774, 837, 1046, 1173
SsiI CCGC 1 cut(s) 1094
SspMI CTAG 1 cut(s) 1220
SstI GAGCTC 1 cut(s) 302
StyD4I CCNGG 2 cut(s) 240, 813
TaaI ACNGT 4 cut(s) 377, 626, 692, 702
TaqI TCGA 1 cut(s) 914
TasI AATT 6 cut(s) 209, 710, 774, 837, 1046, 1173
TauI GCSGC 1 cut(s) 1096
TfiI GAWTC 5 cut(s) 643, 863, 869, 877, 1015
Tru1I TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
Tru9I TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
TscAI CASTG 5 cut(s) 624, 629, 697, 1032, 1138
TseI GCWGC 3 cut(s) 415, 547, 1186
TspDTI ATGAA 5 cut(s) 129, 166, 759, 1058, 1226
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 5 cut(s) 624, 629, 697, 1032, 1138
Van91I CCANNNNNTGG 1 cut(s) 309
Vha464I CTTAAG 1 cut(s) 419
VpaK11BI GGWCC 2 cut(s) 817, 833
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 1 cut(s) 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.