FvH4_6g36930

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011436
Physical Location & Seq
Reverse (-)
28982173 .. 28987199
5027 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36930.t3

Sequence Viewer

Length: 1290 bp
ATGGATTTCTTCAAAGTTAGAAAGTTTAGGAAGGCTCACAAACCAGACCCAGAAAATGAGTTAGAGGACAATCCTGTGCCTGTGCCAGAGGAACCGAAGGAGAATGGTGGTGATGGAAAGGGCAAGGCAGTCAATGTTGATCCTATAGCGGAAGCTGATGACGACGACGATGATTTTATAACAGAGGAAGTCAAGAGGAGATTAAAAGAATTGCGAAGGAATAGCTTTATGGTGCTGATTCCGGAAGAAGAGTCATGTGCTGAAGAGGAGGAAGAGGAAGAGCAGGGAGAGACGAGCTCCAATGAGTGGAGAGATGTAGAAGCAGAAGGTCGGCAATGGTGGTGTGGCTTTGGTGCTGTATATGACAAGTACTGTGAGAGGATGTTGTTCTTTGAACGGATGAGCACACAACAGGTTAAGGAAATTGGTTGTCTGACAGGCATCCAAACCCCTACAACTCCATCACCAAGGTCTGCATCTAAGAAGCTTTCCTCCCCTTTTCGCTGTCTTTCTCTGAAGAAAATTGAAGAACCTAATGACGAGACTGAGCATCTTCAGCAGCCAGAGATCGACCCTTATCATGATATTGAGACATCATACGTAGGTCAAGTTTGCTTGTCTTGGGAGGCACTGCATTGTCAATACACACAACTGAATCAGCTAATCTCATGCCAACCTGAAAATCCCACCTCCTACAACCACAGTGCTCAACAGTTCCAACAATTCCAGGTTTTATTACAAAGGTTTATTGAAAATGAACCTTTTGAAGAGGGTCTCAGGCCAGAAATTTATGCTCGCACGCAGAGAATTCTGCCTCAACTTCTCCGGGTCCCTAACATACAAGGTCCAATTCATAAAGGGAAAGATGAAGAAGAGGCAGATTCCATGATTCTTGCCCCTGATCTGATCAAGATTATTGAAACCTCAATCCTCACATTTTTACTTTTCCTGAAGATGGATAAGAAAAAAACAAGTGCTGGTCTCAGCTTATTTGGAAATCAAAACCATGCTACCCCACTTCAGCAGATTCAAACTTCACTGGATAAGAAATCGATGAAATTAAAGGAACTTCGTAAGAAGAGGAAAAGTTGGAAGAAGTCATGGCCGCAAGCACAAGAAGATGTTCAGCTGTTGTTTAGCCTTGTTGATGTAAAGGTTTTGTCAAGGGTACTCCGGATGGGAAGAATTAGTAAAGAGCAGCTATTTTGGTGTGAGGAGAAGATGAAAAAGCTAGATTTGGTGGATGGCAAGTTGTGGAGAGACCCCTCTCCCACCCTTTTCCCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

430

Amino Acids

49.84

Weight (kDa)

5.21

Isoelectric Point (pI)

58.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 190 - 424 5.3e-91 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 179
AccB7I CCANNNNNTGG 1 cut(s) 306
AccIII TCCGGA 2 cut(s) 241, 1173
AciI CCGC 2 cut(s) 149, 1106
AclWI GGATC 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 1103
AcsI RAATTY 2 cut(s) 786, 807
AcuI CTGAAG 5 cut(s) 282, 536, 539, 971, 1004
AfaI GTAC 2 cut(s) 371, 1170
AfiI CCNNNNNNNGG 2 cut(s) 306, 955
AgsI TTSAA 7 cut(s) 13, 395, 527, 752, 767, 920, 1031
AjnI CCWGG 1 cut(s) 726
AluBI AGCT 9 cut(s) 155, 225, 297, 487, 661, 987, 1129, 1201, 1231
AluI AGCT 9 cut(s) 155, 225, 297, 487, 661, 987, 1129, 1201, 1231
Alw21I GWGCWC 3 cut(s) 299, 407, 709
Alw26I GTCTC 6 cut(s) 284, 536, 584, 779, 986, 1254
AlwI GGATC 1 cut(s) 134
Aor13HI TCCGGA 2 cut(s) 241, 1173
AoxI GGCC 2 cut(s) 779, 1103
ApeKI GCWGC 2 cut(s) 559, 1198
ApoI RAATTY 2 cut(s) 786, 807
Asp700I GAANNNNTTC 1 cut(s) 1122
AspS9I GGNCC 2 cut(s) 829, 845
AsuC2I CCSGG 1 cut(s) 827
AsuHPI GGTGA 2 cut(s) 122, 456
AvaII GGWCC 2 cut(s) 829, 845
BanII GRGCYC 1 cut(s) 299
Bbv12I GWGCWC 3 cut(s) 299, 407, 709
BbvI GCAGC 2 cut(s) 571, 1210
BccI CCATC 5 cut(s) 107, 469, 949, 1171, 1238
BciT130I CCWGG 1 cut(s) 728
BclI TGATCA 1 cut(s) 906
BcnI CCSGG 1 cut(s) 827
BcoDI GTCTC 6 cut(s) 284, 536, 584, 779, 986, 1254
BfaI CTAG 1 cut(s) 1232
BfmI CTRYAG 1 cut(s) 144
BisI GCNGC 3 cut(s) 560, 1106, 1199
BlsI GCNGC 3 cut(s) 561, 1107, 1200
BmcAI AGTACT 1 cut(s) 371
Bme1390I CCNGG 2 cut(s) 728, 827
Bme18I GGWCC 2 cut(s) 829, 845
BmgT120I GGNCC 2 cut(s) 829, 845
BmiI GGNNCC 3 cut(s) 93, 830, 831
BmrFI CCNGG 2 cut(s) 728, 827
BmsI GCATC 3 cut(s) 450, 485, 559
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpuMI CCSGG 1 cut(s) 827
Bsa29I ATCGAT 1 cut(s) 1052
BsaAI YACGTR 1 cut(s) 601
BsaI GGTCTC 3 cut(s) 779, 986, 1254
BsaJI CCNNGG 1 cut(s) 467
BsaWI WCCGGW 2 cut(s) 241, 1173
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 955
Bse1I ACTGG 1 cut(s) 1044
Bse3DI GCAATG 1 cut(s) 341
BseAI TCCGGA 2 cut(s) 241, 1173
BseBI CCWGG 1 cut(s) 728
BseCI ATCGAT 1 cut(s) 1052
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 5 cut(s) 387, 405, 441, 1182, 1249
BseLI CCNNNNNNNGG 2 cut(s) 306, 955
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 3 cut(s) 537, 790, 997
BseNI ACTGG 1 cut(s) 1044
BseRI GAGGAG 3 cut(s) 211, 281, 1229
BseXI GCAGC 2 cut(s) 571, 1210
BshFI GGCC 2 cut(s) 781, 1105
BshVI ATCGAT 1 cut(s) 1052
BsiHKAI GWGCWC 3 cut(s) 299, 407, 709
BsiSI CCGG 3 cut(s) 242, 826, 1174
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 2 cut(s) 306, 955
BsmAI GTCTC 6 cut(s) 284, 536, 584, 779, 986, 1254
BsmBI CGTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 815
BsnI GGCC 2 cut(s) 781, 1105
Bso31I GGTCTC 3 cut(s) 779, 986, 1254
Bsp1286I GDGCHC 3 cut(s) 299, 407, 709
Bsp13I TCCGGA 2 cut(s) 241, 1173
Bsp143I GATC 4 cut(s) 139, 567, 901, 906
BspACI CCGC 2 cut(s) 149, 1106
BspANI GGCC 2 cut(s) 781, 1105
BspCNI CTCAG 3 cut(s) 538, 789, 996
BspDI ATCGAT 1 cut(s) 1052
BspEI TCCGGA 2 cut(s) 241, 1173
BspHI TCATGA 1 cut(s) 580
BspLI GGNNCC 3 cut(s) 93, 830, 831
BspPI GGATC 1 cut(s) 134
BspQI GCTCTTC 1 cut(s) 273
BspTNI GGTCTC 3 cut(s) 779, 986, 1254
BsrDI GCAATG 1 cut(s) 341
BsrI ACTGG 1 cut(s) 1044
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 4 cut(s) 139, 567, 901, 906
BssT1I CCWWGG 1 cut(s) 467
Bst2UI CCWGG 1 cut(s) 728
Bst4CI ACNGT 3 cut(s) 374, 704, 714
Bst6I CTCTTC 7 cut(s) 243, 258, 267, 273, 762, 867, 1073
BstBAI YACGTR 1 cut(s) 601
BstC8I GCNNGC 3 cut(s) 796, 800, 1110
BstDEI CTNAG 4 cut(s) 480, 546, 776, 983
BstF5I GGATG 5 cut(s) 387, 405, 441, 1182, 1249
BstKTI GATC 4 cut(s) 142, 570, 904, 909
BstMAI GTCTC 6 cut(s) 284, 536, 584, 779, 986, 1254
BstMBI GATC 4 cut(s) 139, 567, 901, 906
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstNI CCWGG 1 cut(s) 728
BstSCI CCNGG 2 cut(s) 726, 825
BstSFI CTRYAG 1 cut(s) 144
BstSNI TACGTA 1 cut(s) 601
BstV1I GCAGC 2 cut(s) 571, 1210
Bsu15I ATCGAT 1 cut(s) 1052
BsuRI GGCC 2 cut(s) 781, 1105
BsuTUI ATCGAT 1 cut(s) 1052
BtsCI GGATG 5 cut(s) 387, 405, 441, 1182, 1249
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 3 cut(s) 629, 709, 1037
Cac8I GCNNGC 3 cut(s) 796, 800, 1110
CciI TCATGA 1 cut(s) 580
Cfr13I GGNCC 2 cut(s) 829, 845
ClaI ATCGAT 1 cut(s) 1052
Csp6I GTAC 2 cut(s) 370, 1169
CviAII CATG 6 cut(s) 255, 581, 669, 886, 1007, 1101
CviQI GTAC 2 cut(s) 370, 1169
DdeI CTNAG 4 cut(s) 480, 546, 776, 983
DpnI GATC 4 cut(s) 141, 569, 903, 908
DpnII GATC 4 cut(s) 139, 567, 901, 906
EaeI YGGCCR 1 cut(s) 1103
Eam1104I CTCTTC 7 cut(s) 243, 258, 267, 273, 762, 867, 1073
EarI CTCTTC 7 cut(s) 243, 258, 267, 273, 762, 867, 1073
Ecl136II GAGCTC 1 cut(s) 297
Eco105I TACGTA 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 467
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 3 cut(s) 779, 986, 1254
Eco47I GGWCC 2 cut(s) 829, 845
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 5 cut(s) 282, 536, 539, 971, 1004
EcoICRI GAGCTC 1 cut(s) 297
EcoO109I RGGNCCY 1 cut(s) 829
EcoRI GAATTC 1 cut(s) 807
EcoRII CCWGG 1 cut(s) 726
EcoT14I CCWWGG 1 cut(s) 467
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 467
Esp3I CGTCTC 1 cut(s) 284
FaeI CATG 6 cut(s) 258, 584, 672, 889, 1010, 1104
FaqI GGGAC 1 cut(s) 815
FatI CATG 6 cut(s) 254, 580, 668, 885, 1006, 1100
FbaI TGATCA 1 cut(s) 906
Fnu4HI GCNGC 3 cut(s) 560, 1106, 1199
FokI GGATG 5 cut(s) 394, 412, 428, 1189, 1256
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 3 cut(s) 560, 1106, 1199
FspBI CTAG 1 cut(s) 1232
GluI GCNGC 3 cut(s) 560, 1106, 1199
HaeIII GGCC 2 cut(s) 781, 1105
HapII CCGG 3 cut(s) 242, 826, 1174
Hin1II CATG 6 cut(s) 258, 584, 672, 889, 1010, 1104
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 6 cut(s) 238, 251, 655, 881, 889, 1027
HpaII CCGG 3 cut(s) 242, 826, 1174
HphI GGTGA 2 cut(s) 122, 456
Hpy188I TCNGA 3 cut(s) 435, 516, 906
Hpy188III TCNNGA 6 cut(s) 193, 242, 581, 910, 949, 1174
Hpy99I CGWCG 2 cut(s) 167, 170
HpyAV CCTTC 4 cut(s) 25, 91, 210, 320
HpyCH4III ACNGT 3 cut(s) 374, 704, 714
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 2 cut(s) 476, 634
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 4 cut(s) 480, 546, 776, 983
HpySE526I ACGT 1 cut(s) 600
Hsp92II CATG 6 cut(s) 258, 584, 672, 889, 1010, 1104
KflI GGGWCCC 1 cut(s) 829
Kpn2I TCCGGA 2 cut(s) 241, 1173
Ksp22I TGATCA 1 cut(s) 906
Kzo9I GATC 4 cut(s) 139, 567, 901, 906
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 302
Lsp1109I GCAGC 2 cut(s) 571, 1210
LweI GCATC 3 cut(s) 450, 485, 559
MaeI CTAG 1 cut(s) 1232
MaeII ACGT 1 cut(s) 600
MalI GATC 4 cut(s) 141, 569, 903, 908
MboI GATC 4 cut(s) 139, 567, 901, 906
MhlI GDGCHC 3 cut(s) 299, 407, 709
MluCI AATT 9 cut(s) 209, 423, 522, 722, 786, 807, 849, 1058, 1185
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 742, 1070
MroI TCCGGA 2 cut(s) 241, 1173
MroXI GAANNNNTTC 1 cut(s) 1122
MseI TTAA 4 cut(s) 203, 417, 1061, 1288
MspA1I CMGCKG 1 cut(s) 1129
MspI CCGG 3 cut(s) 242, 826, 1174
MspR9I CCNGG 2 cut(s) 728, 827
MvaI CCWGG 1 cut(s) 728
MwoI GCNNNNNNNGC 1 cut(s) 556
NciI CCSGG 1 cut(s) 827
NdeII GATC 4 cut(s) 139, 567, 901, 906
NlaIII CATG 6 cut(s) 258, 584, 672, 889, 1010, 1104
NlaIV GGNNCC 3 cut(s) 93, 830, 831
PagI TCATGA 1 cut(s) 580
PciSI GCTCTTC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 1122
PfeI GAWTC 5 cut(s) 238, 655, 881, 889, 1027
PflMI CCANNNNNTGG 1 cut(s) 306
PkrI GCNGC 3 cut(s) 561, 1107, 1200
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
Ppu21I YACGTR 1 cut(s) 601
PpuMI RGGWCCY 1 cut(s) 829
PsiI TTATAA 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 299
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 1 cut(s) 726
PspGI CCWGG 1 cut(s) 726
PspN4I GGNNCC 3 cut(s) 93, 830, 831
PspPI GGNCC 2 cut(s) 829, 845
PspPPI RGGWCCY 1 cut(s) 829
PvuII CAGCTG 1 cut(s) 1129
RsaI GTAC 2 cut(s) 371, 1170
RsaNI GTAC 2 cut(s) 370, 1169
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 4 cut(s) 203, 417, 1061, 1288
SatI GCNGC 3 cut(s) 560, 1106, 1199
Sau3AI GATC 4 cut(s) 139, 567, 901, 906
Sau96I GGNCC 2 cut(s) 829, 845
ScaI AGTACT 1 cut(s) 371
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 728, 827
SduI GDGCHC 3 cut(s) 299, 407, 709
SfaNI GCATC 3 cut(s) 450, 485, 559
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 2 cut(s) 829, 845
SnaBI TACGTA 1 cut(s) 601
Sse9I AATT 9 cut(s) 209, 423, 522, 722, 786, 807, 849, 1058, 1185
SsiI CCGC 2 cut(s) 149, 1106
SspMI CTAG 1 cut(s) 1232
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 2 cut(s) 726, 825
StyI CCWWGG 1 cut(s) 467
TaaI ACNGT 3 cut(s) 374, 704, 714
TaiI ACGT 1 cut(s) 603
TaqI TCGA 2 cut(s) 570, 1052
TasI AATT 9 cut(s) 209, 423, 522, 722, 786, 807, 849, 1058, 1185
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 1108
TfiI GAWTC 5 cut(s) 238, 655, 881, 889, 1027
Tru1I TTAA 4 cut(s) 203, 417, 1061, 1288
Tru9I TTAA 4 cut(s) 203, 417, 1061, 1288
TscAI CASTG 3 cut(s) 636, 709, 1044
TseI GCWGC 2 cut(s) 559, 1198
TspDTI ATGAA 5 cut(s) 771, 842, 882, 1070, 1238
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 3 cut(s) 636, 709, 1044
Van91I CCANNNNNTGG 1 cut(s) 306
VpaK11BI GGWCC 2 cut(s) 829, 845
XapI RAATTY 2 cut(s) 786, 807
XmnI GAANNNNTTC 1 cut(s) 1122
XspI CTAG 1 cut(s) 1232
ZrmI AGTACT 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.