MD17G1162200.v1.1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011436
Physical Location & Seq
Reverse (-)
16028769 .. 16032914
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1162200.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGATTTCTTCAAAGTTAAGAAGTTTAGAAAAGCACACAAACCAGACCCAGAAAATGTGTCGGAGGACAGTCCCGTGCCACAGCCGGAGGAACCCAACAAGAAGAATAGTGGTGATGATTTTGGTAAGTCGGCCAATGCTGATCCTGTGGCCGAGGCTGAGGACGATGATGATGATTTCATAATTGAGGAGGTCAAGCGGAAGTTAAAAGAATTGAGAAGGAACAGCTTTATGGAGCTGATTCCGGAAGAAGAGTCATTACCCGAAGAGGAAGAAGAAGAAGAAGCCGGTGAGACAAGCTCTAATGAGTGGAGGGATGTAGAAGCAGAAGGCCAGCTACGGTGGTGTGGCTTTGTTGCCGTATATGACAAATACTGGGAAAGGATGTTGTTCTTTGAGCAGATGAGTGCGCAGCATCTTAGTGGAACTGGCATCCAAACACCCACGGCTCCATCACCGCGATCTGCATCTAAGAAGCTTACCTCCCCTTTTCACTGTCTTTCTTTAAAGAAGATTGAAGAGCCTGATAATGAGACTGAGCACCTTCAGCCGCCTGATGATGATCCTTACCAGGATATCGAGACATCATATGTAGGTCAAATTTGCTTGTCTTGGGAGGCACTGCACTGTCAGTACACTCAGCTAAACCAGCTAATTGCATGCCAACCTGAAAATCCCAACAGTTACAACCACAGTGCTCAACAATTTCAACAGTTCCAGGTTTTACTACAACGGTATATCGAAAATGAACCCTTTGAGGAGGGCCACAGGGCCGAAATTTACGCTCGCACTCAAAGACTTCTTCCTAATTTACTCCGGGTGCCTAATATACAAGGTCCAATACAAAAAGGAAAGGAGGAAGAAGAATCAGATTCGTCGGTGCATGCTCCTGATCTCATCAAGATTATTGAAACCTCAATCCTTACTTTTCAGCTTTTCCTGAAGATGGATAAGAAAAAACCGAGCTCTGTTCTTAACTTGTTTGGGAATCAAAACCAGGCTGCCACCCCACTTCAGCAGATTCAAACTTCTCTCCAGAAGAAATGGATGAAGTTAAAGGAACTGCGCAAAAAGAGGAAAGGTTGGAAGAAGAAATTGTGGCCGCAATCACAGGAAGATGTTCAGCTTTTGTTCAGCCTCATTGACGCCAAGGTTTTGTCAAGAACCCTCCGGATGGTGAGAATAAGTAAAGAGCAGCTGTTTTGGTGCGAGGAAAAGATGAAAAAACTCGACTTAGTTGATGGCAAGTTGTGGAGAGACCTGTCTCCCACCCTGTTCCCCTGTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

430

Amino Acids

49.94

Weight (kDa)

5.19

Isoelectric Point (pI)

63.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 191 - 427 8.2e-91 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 411, 1067
AccB1I GGYRCC 1 cut(s) 820
AccII CGCG 1 cut(s) 460
AccIII TCCGGA 2 cut(s) 244, 1170
AciI CCGC 4 cut(s) 199, 458, 551, 1103
AclWI GGATC 2 cut(s) 137, 557
AcoI YGGCCR 3 cut(s) 132, 150, 1100
AcsI RAATTY 2 cut(s) 600, 777
AcuI CTGAAG 3 cut(s) 530, 962, 998
AcyI GRCGYC 1 cut(s) 1146
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 946, 1174
AgsI TTSAA 5 cut(s) 13, 518, 710, 911, 1025
AjnI CCWGG 3 cut(s) 570, 717, 996
Alw21I GWGCWC 3 cut(s) 543, 700, 968
Alw26I GTCTC 5 cut(s) 287, 527, 575, 1251, 1269
AlwI GGATC 2 cut(s) 137, 557
Aor13HI TCCGGA 2 cut(s) 244, 1170
AoxI GGCC 6 cut(s) 132, 150, 331, 763, 771, 1100
ApeKI GCWGC 3 cut(s) 412, 1001, 1195
ApoI RAATTY 2 cut(s) 600, 777
Asp700I GAANNNNTTC 1 cut(s) 1119
AspLEI GCGC 2 cut(s) 412, 1068
AspS9I GGNCC 3 cut(s) 763, 771, 836
AsuC2I CCSGG 1 cut(s) 818
AsuHPI GGTGA 4 cut(s) 125, 302, 447, 1189
AvaII GGWCC 1 cut(s) 836
BanI GGYRCC 1 cut(s) 820
BanII GRGCYC 1 cut(s) 968
BarI GAAGNNNNNNTAC 4 cut(s) 243, 275, 321, 353
Bbv12I GWGCWC 3 cut(s) 543, 700, 968
BbvCI CCTCAGC 1 cut(s) 159
BbvI GCAGC 3 cut(s) 424, 988, 1207
BccI CCATC 4 cut(s) 460, 940, 1168, 1235
BceAI ACGGC 2 cut(s) 344, 462
BciT130I CCWGG 3 cut(s) 572, 719, 998
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 5 cut(s) 287, 527, 575, 1251, 1269
BisI GCNGC 5 cut(s) 413, 551, 1002, 1103, 1196
BlsI GCNGC 5 cut(s) 414, 552, 1003, 1104, 1197
Bme1390I CCNGG 4 cut(s) 572, 719, 818, 998
Bme18I GGWCC 1 cut(s) 836
BmgT120I GGNCC 3 cut(s) 763, 771, 836
BmiI GGNNCC 3 cut(s) 93, 450, 822
BmrFI CCNGG 4 cut(s) 572, 719, 818, 998
BmrI ACTGGG 1 cut(s) 385
BmsI GCATC 3 cut(s) 424, 441, 476
BmuI ACTGGG 1 cut(s) 385
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpmI CTGGAG 1 cut(s) 1019
Bpu10I CCTNAGC 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 818
BsaBI GATNNNNATC 2 cut(s) 466, 561
BsaHI GRCGYC 1 cut(s) 1146
BsaI GGTCTC 1 cut(s) 1251
BsaJI CCNNGG 3 cut(s) 153, 444, 1149
BsaWI WCCGGW 2 cut(s) 244, 1170
Bsc4I CCNNNNNNNGG 2 cut(s) 946, 1174
Bse118I RCCGGY 1 cut(s) 287
Bse1I ACTGG 2 cut(s) 380, 433
Bse8I GATNNNNATC 2 cut(s) 466, 561
BseAI TCCGGA 2 cut(s) 244, 1170
BseBI CCWGG 3 cut(s) 572, 719, 998
BseDI CCNNGG 3 cut(s) 153, 444, 1149
BseGI GGATG 5 cut(s) 322, 390, 432, 1053, 1179
BseJI GATNNNNATC 2 cut(s) 466, 561
BseLI CCNNNNNNNGG 2 cut(s) 946, 1174
BseMII CTCAG 3 cut(s) 150, 528, 653
BseNI ACTGG 2 cut(s) 380, 433
BseRI GAGGAG 2 cut(s) 203, 773
BseXI GCAGC 3 cut(s) 424, 988, 1207
BsgI GTGCAG 1 cut(s) 608
Bsh1236I CGCG 1 cut(s) 460
BshFI GGCC 6 cut(s) 134, 152, 333, 765, 773, 1102
BshNI GGYRCC 1 cut(s) 820
BsiHKAI GWGCWC 3 cut(s) 543, 700, 968
BsiSI CCGG 5 cut(s) 86, 245, 288, 817, 1171
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 946, 1174
BsmAI GTCTC 5 cut(s) 287, 527, 575, 1251, 1269
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 6 cut(s) 134, 152, 333, 765, 773, 1102
Bso31I GGTCTC 1 cut(s) 1251
Bsp1286I GDGCHC 3 cut(s) 543, 700, 968
Bsp13I TCCGGA 2 cut(s) 244, 1170
Bsp143I GATC 4 cut(s) 142, 461, 562, 892
BspACI CCGC 4 cut(s) 199, 458, 551, 1103
BspANI GGCC 6 cut(s) 134, 152, 333, 765, 773, 1102
BspCNI CTCAG 3 cut(s) 151, 529, 652
BspEI TCCGGA 2 cut(s) 244, 1170
BspFNI CGCG 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 93, 450, 822
BspPI GGATC 2 cut(s) 137, 557
BspQI GCTCTTC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 820
BspTNI GGTCTC 1 cut(s) 1251
BsrFI RCCGGY 1 cut(s) 287
BsrI ACTGG 2 cut(s) 380, 433
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 3 cut(s) 153, 444, 1149
BssMI GATC 4 cut(s) 142, 461, 562, 892
BssNI GRCGYC 1 cut(s) 1146
BssT1I CCWWGG 1 cut(s) 1149
Bst2UI CCWGG 3 cut(s) 572, 719, 998
Bst4CI ACNGT 8 cut(s) 71, 342, 497, 629, 683, 695, 714, 735
Bst6I CTCTTC 3 cut(s) 246, 261, 513
BstACI GRCGYC 1 cut(s) 1146
BstC8I GCNNGC 4 cut(s) 335, 661, 787, 885
BstDEI CTNAG 6 cut(s) 159, 419, 471, 537, 639, 1234
BstDSI CCRYGG 1 cut(s) 444
BstF5I GGATG 5 cut(s) 322, 390, 432, 1053, 1179
BstFNI CGCG 1 cut(s) 460
BstHHI GCGC 2 cut(s) 412, 1068
BstKTI GATC 4 cut(s) 145, 464, 565, 895
BstMAI GTCTC 5 cut(s) 287, 527, 575, 1251, 1269
BstMBI GATC 4 cut(s) 142, 461, 562, 892
BstMWI GCNNNNNNNGC 2 cut(s) 547, 649
BstNI CCWGG 3 cut(s) 572, 719, 998
BstNSI RCATGY 2 cut(s) 663, 887
BstSCI CCNGG 4 cut(s) 570, 717, 816, 996
BstUI CGCG 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 424, 988, 1207
BsuRI GGCC 6 cut(s) 134, 152, 333, 765, 773, 1102
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 5 cut(s) 322, 390, 432, 1053, 1179
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 4 cut(s) 493, 620, 625, 700
Cac8I GCNNGC 4 cut(s) 335, 661, 787, 885
CfoI GCGC 2 cut(s) 412, 1068
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 3 cut(s) 763, 771, 836
CseI GACGC 1 cut(s) 1154
Csp6I GTAC 1 cut(s) 634
CviAII CATG 2 cut(s) 660, 884
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 6 cut(s) 159, 419, 471, 537, 639, 1234
DpnI GATC 4 cut(s) 144, 463, 564, 894
DpnII GATC 4 cut(s) 142, 461, 562, 892
DraI TTTAAA 1 cut(s) 507
EaeI YGGCCR 3 cut(s) 132, 150, 1100
Eam1104I CTCTTC 3 cut(s) 246, 261, 513
EarI CTCTTC 3 cut(s) 246, 261, 513
Ecl136II GAGCTC 1 cut(s) 966
Eco130I CCWWGG 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 968
Eco31I GGTCTC 1 cut(s) 1251
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 836
Eco53kI GAGCTC 1 cut(s) 966
Eco57I CTGAAG 3 cut(s) 530, 962, 998
EcoICRI GAGCTC 1 cut(s) 966
EcoRII CCWGG 3 cut(s) 570, 717, 996
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 1 cut(s) 1149
EcoT38I GRGCYC 1 cut(s) 968
ErhI CCWWGG 1 cut(s) 1149
FaeI CATG 2 cut(s) 663, 887
FaqI GGGAC 1 cut(s) 57
FatI CATG 2 cut(s) 659, 883
FauNDI CATATG 1 cut(s) 589
Fnu4HI GCNGC 5 cut(s) 413, 551, 1002, 1103, 1196
FokI GGATG 5 cut(s) 329, 397, 419, 1060, 1186
FriOI GRGCYC 1 cut(s) 968
Fsp4HI GCNGC 5 cut(s) 413, 551, 1002, 1103, 1196
FspI TGCGCA 2 cut(s) 411, 1067
GlaI GCGC 2 cut(s) 411, 1067
GluI GCNGC 5 cut(s) 413, 551, 1002, 1103, 1196
GsuI CTGGAG 1 cut(s) 1019
HaeIII GGCC 6 cut(s) 134, 152, 333, 765, 773, 1102
HapII CCGG 5 cut(s) 86, 245, 288, 817, 1171
HgaI GACGC 1 cut(s) 1154
HhaI GCGC 2 cut(s) 412, 1068
Hin1I GRCGYC 1 cut(s) 1146
Hin1II CATG 2 cut(s) 663, 887
Hin6I GCGC 2 cut(s) 410, 1066
HinP1I GCGC 2 cut(s) 410, 1066
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 6 cut(s) 241, 254, 866, 872, 988, 1021
HpaII CCGG 5 cut(s) 86, 245, 288, 817, 1171
HphI GGTGA 4 cut(s) 125, 302, 447, 1189
Hpy166II GTNNAC 1 cut(s) 636
Hpy188I TCNGA 2 cut(s) 64, 871
Hpy188III TCNNGA 8 cut(s) 245, 580, 890, 901, 940, 1036, 1161, 1171
Hpy8I GTNNAC 1 cut(s) 636
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 3 cut(s) 213, 323, 554
HpyCH4III ACNGT 8 cut(s) 71, 342, 497, 629, 683, 695, 714, 735
HpyCH4V TGCA 4 cut(s) 467, 625, 659, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 547, 649
HpyF3I CTNAG 6 cut(s) 159, 419, 471, 537, 639, 1234
Hsp92I GRCGYC 1 cut(s) 1146
Hsp92II CATG 2 cut(s) 663, 887
HspAI GCGC 2 cut(s) 410, 1066
Kpn2I TCCGGA 2 cut(s) 244, 1170
Kzo9I GATC 4 cut(s) 142, 461, 562, 892
LguI GCTCTTC 1 cut(s) 513
LmnI GCTCC 3 cut(s) 235, 454, 892
Lsp1109I GCAGC 3 cut(s) 424, 988, 1207
LweI GCATC 3 cut(s) 424, 441, 476
MaeIII GTNAC 1 cut(s) 683
MalI GATC 4 cut(s) 144, 463, 564, 894
MboI GATC 4 cut(s) 142, 461, 562, 892
MhlI GDGCHC 3 cut(s) 543, 700, 968
MluCI AATT 8 cut(s) 183, 212, 600, 654, 704, 777, 808, 1094
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 2 cut(s) 42, 1064
MroI TCCGGA 2 cut(s) 244, 1170
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 5 cut(s) 18, 206, 506, 975, 1055
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 1198
MspI CCGG 5 cut(s) 86, 245, 288, 817, 1171
MspR9I CCNGG 4 cut(s) 572, 719, 818, 998
MvaI CCWGG 3 cut(s) 572, 719, 998
MvnI CGCG 1 cut(s) 460
MwoI GCNNNNNNNGC 2 cut(s) 547, 649
NciI CCSGG 1 cut(s) 818
NdeI CATATG 1 cut(s) 589
NdeII GATC 4 cut(s) 142, 461, 562, 892
NlaIII CATG 2 cut(s) 663, 887
NlaIV GGNNCC 3 cut(s) 93, 450, 822
NmeAIII GCCGAG 1 cut(s) 178
NsbI TGCGCA 2 cut(s) 411, 1067
NspI RCATGY 2 cut(s) 663, 887
PaeI GCATGC 2 cut(s) 663, 887
PciSI GCTCTTC 1 cut(s) 513
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 5 cut(s) 241, 866, 872, 988, 1021
PkrI GCNGC 5 cut(s) 414, 552, 1003, 1104, 1197
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 968
Psp6I CCWGG 3 cut(s) 570, 717, 996
PspGI CCWGG 3 cut(s) 570, 717, 996
PspN4I GGNNCC 3 cut(s) 93, 450, 822
PspPI GGNCC 3 cut(s) 763, 771, 836
PvuII CAGCTG 1 cut(s) 1198
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 420
SacI GAGCTC 1 cut(s) 968
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 5 cut(s) 18, 206, 506, 975, 1055
SatI GCNGC 5 cut(s) 413, 551, 1002, 1103, 1196
Sau3AI GATC 4 cut(s) 142, 461, 562, 892
Sau96I GGNCC 3 cut(s) 763, 771, 836
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 4 cut(s) 572, 719, 818, 998
SduI GDGCHC 3 cut(s) 543, 700, 968
SfaNI GCATC 3 cut(s) 424, 441, 476
SinI GGWCC 1 cut(s) 836
SmiMI CAYNNNNRTG 1 cut(s) 420
SphI GCATGC 2 cut(s) 663, 887
Sse9I AATT 8 cut(s) 183, 212, 600, 654, 704, 777, 808, 1094
SsiI CCGC 4 cut(s) 199, 458, 551, 1103
SstI GAGCTC 1 cut(s) 968
StyD4I CCNGG 4 cut(s) 570, 717, 816, 996
StyI CCWWGG 1 cut(s) 1149
TaaI ACNGT 8 cut(s) 71, 342, 497, 629, 683, 695, 714, 735
TaqI TCGA 3 cut(s) 579, 741, 1230
TasI AATT 8 cut(s) 183, 212, 600, 654, 704, 777, 808, 1094
TatI WGTACW 1 cut(s) 633
TauI GCSGC 2 cut(s) 553, 1105
TfiI GAWTC 5 cut(s) 241, 866, 872, 988, 1021
Tru1I TTAA 5 cut(s) 18, 206, 506, 975, 1055
Tru9I TTAA 5 cut(s) 18, 206, 506, 975, 1055
TscAI CASTG 4 cut(s) 500, 627, 632, 700
TseI GCWGC 3 cut(s) 412, 1001, 1195
TspDTI ATGAA 4 cut(s) 169, 762, 1064, 1235
TspRI CASTG 4 cut(s) 500, 627, 632, 700
VpaK11BI GGWCC 1 cut(s) 836
XapI RAATTY 2 cut(s) 600, 777
XceI RCATGY 2 cut(s) 663, 887
XmnI GAANNNNTTC 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.