Rroxscaffold_2G00098170

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011436
Physical Location & Seq
Forward (+)
19347762 .. 19352411
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00098170.1

Sequence Viewer

Length: 1278 bp
ATGGATTTCTTCAAAGTTAAGAAGTTTAGAAAAGCTCACAAACCAGACCCAGAAAATGAGTTAGAGGACAATCCTGTGCCTGTGCCAGAGGAACCAAAGGAGAATGGTGGTGATGAAAAAGGCAAGGCCGTTAATGTTGATCCTATAGCTGAAGCTGACGATGACGATGATGATTTCATAACAGAGGAAGTCAAGAGAAGGTTAAAAGAATTGCGAAGGAATAGTTTTATGGTATTGATCCCGGAAGAAGAGTCATGTGCTGAAGAGGAGGAGGAAGAAGAAGAGCAGGGAGAGACAAGCTCCAATGAGTGGAGGGATGTAGAAGCTGAGGGTCGGCAATGGTGGTGTGGCTTTGGTGCTGTATATGACAAATACTGTGAGAGGATGTTGTTCTTTGAACGGATGAGCACACAGCAGCTTAAGGAAGGCATCCAAACCCCTACAACTCCATCACCAAGATCTGCATCTAAGAAGCTTTCCTCTCCTTTTCGCTGTCTCTCTTTGAAGAAGACTGAAGAACCTAATGACGAGACTGAGCATCTCCAGCAGCCAGAGATGGACCCTTATCATGATATTGAGACATCATATGTAGCTCAAGTTTGCTTGTCTTGGGAGGCACTGCACTGTCAATACACTCAACTGAATCAGCTAATCTCATGCCAACCTGAAAATCCCACCTCCTACAACCACAGTGCTCAACAGTTTCAACAATTTCAGGTTTTATTACAAAGGTTTATTGAAAATGAACCTTTTGAGGAGGGTCTCAGGCCAGAAATTTATGCTCGCACGCAGAGAGTTCTGCCTAAACTACTCCGGGTCCCCAATATACAAGGTCCAATTAATAAAGGGAAAGAAGAAGAAGAGTCAGATTCCATGATTCTTGCCCCTGATCTGATCAAGATTATTGAAACCTCAATCCTCACATTTCTACTTTTCCTGAAGATGGATAAGAAAAAAACAAGCGCTGGTCTTAGCTTATTTGGAAATCAAAACCATGCTACCCCACTTCAGCAGATTCAAACTTCACTGGATAAGAAATGGATGAAATTAAAGGAACTTCGTAAAAAGAGGAAAAGTTGGAAGAAGTCATGGCCACAATCACAAGAAGATGTTCAGCTGTTGTTTAGCCTCACTGATGCAAAGGTTTTGTCAAGGGTTCTCCGGATGGGAAGAATTAGTAAAGAGCAGCTATTTTGGTGCGAGGAAAAGATGAAAAAGCTAGATTTGGTGGATGGCAAGTTGTGGAGAGACCCCTCTCCCACCCTTTTCCCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

425

Amino Acids

49.64

Weight (kDa)

5.14

Isoelectric Point (pI)

60.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 190 - 424 2e-89 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 309
AccIII TCCGGA 1 cut(s) 1161
AclWI GGATC 2 cut(s) 134, 232
AcoI YGGCCR 1 cut(s) 1091
AcsI RAATTY 1 cut(s) 774
AcuI CTGAAG 5 cut(s) 171, 282, 534, 959, 992
AfeI AGCGCT 1 cut(s) 964
AfiI CCNNNNNNNGG 2 cut(s) 309, 943
AflII CTTAAG 1 cut(s) 419
AgsI TTSAA 7 cut(s) 13, 398, 505, 707, 740, 908, 1019
Alw21I GWGCWC 2 cut(s) 410, 697
Alw26I GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
AlwI GGATC 2 cut(s) 134, 232
Aor13HI TCCGGA 1 cut(s) 1161
Aor51HI AGCGCT 1 cut(s) 964
AoxI GGCC 3 cut(s) 126, 767, 1091
ApeKI GCWGC 3 cut(s) 415, 547, 1186
ApoI RAATTY 1 cut(s) 774
ArsI GACNNNNNNTTYG 2 cut(s) 1133, 1165
AseI ATTAAT 1 cut(s) 840
Asp700I GAANNNNTTC 1 cut(s) 1110
AspLEI GCGC 1 cut(s) 965
AspS9I GGNCC 3 cut(s) 559, 817, 833
AsuC2I CCSGG 2 cut(s) 242, 815
AsuHPI GGTGA 2 cut(s) 122, 444
AvaII GGWCC 3 cut(s) 559, 817, 833
BalI TGGCCA 1 cut(s) 1093
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 2 cut(s) 410, 697
BbvCI CCTCAGC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 427, 559, 1198
BccI CCATC 5 cut(s) 457, 550, 937, 1159, 1226
BceAI ACGGC 1 cut(s) 113
BclI TGATCA 1 cut(s) 894
BcnI CCSGG 2 cut(s) 242, 815
BcoDI GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
BfaI CTAG 1 cut(s) 1220
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 1 cut(s) 966
BfrI CTTAAG 1 cut(s) 419
BglII AGATCT 1 cut(s) 458
BisI GCNGC 3 cut(s) 416, 548, 1187
BlsI GCNGC 3 cut(s) 417, 549, 1188
Bme1390I CCNGG 2 cut(s) 242, 815
Bme18I GGWCC 3 cut(s) 559, 817, 833
BmgT120I GGNCC 3 cut(s) 559, 817, 833
BmiI GGNNCC 4 cut(s) 93, 561, 818, 819
BmrFI CCNGG 2 cut(s) 242, 815
BmsI GCATC 4 cut(s) 438, 473, 547, 1126
BpiI GAAGAC 1 cut(s) 515
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 579
BpuMI CCSGG 2 cut(s) 242, 815
BsaBI GATNNNNATC 1 cut(s) 463
BsaI GGTCTC 2 cut(s) 767, 1242
BsaWI WCCGGW 1 cut(s) 1161
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 943
Bse1I ACTGG 1 cut(s) 1032
Bse3DI GCAATG 1 cut(s) 344
Bse8I GATNNNNATC 1 cut(s) 463
BseAI TCCGGA 1 cut(s) 1161
BseGI GGATG 7 cut(s) 322, 390, 408, 429, 1047, 1170, 1237
BseJI GATNNNNATC 1 cut(s) 463
BseLI CCNNNNNNNGG 2 cut(s) 309, 943
BseMI GCAATG 1 cut(s) 344
BseMII CTCAG 3 cut(s) 318, 525, 778
BseNI ACTGG 1 cut(s) 1032
BseRI GAGGAG 3 cut(s) 281, 284, 770
BseXI GCAGC 3 cut(s) 427, 559, 1198
BsgI GTGCAG 1 cut(s) 605
BshFI GGCC 3 cut(s) 128, 769, 1093
BsiHKAI GWGCWC 2 cut(s) 410, 697
BsiSI CCGG 3 cut(s) 242, 814, 1162
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 2 cut(s) 309, 943
BsmAI GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
BsmFI GGGAC 1 cut(s) 803
BsnI GGCC 3 cut(s) 128, 769, 1093
Bso31I GGTCTC 2 cut(s) 767, 1242
Bsp1286I GDGCHC 2 cut(s) 410, 697
Bsp13I TCCGGA 1 cut(s) 1161
Bsp143I GATC 5 cut(s) 139, 237, 458, 889, 894
BspANI GGCC 3 cut(s) 128, 769, 1093
BspCNI CTCAG 3 cut(s) 319, 526, 777
BspEI TCCGGA 1 cut(s) 1161
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 4 cut(s) 93, 561, 818, 819
BspPI GGATC 2 cut(s) 134, 232
BspQI GCTCTTC 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 419
BspTNI GGTCTC 2 cut(s) 767, 1242
BsrDI GCAATG 1 cut(s) 344
BsrI ACTGG 1 cut(s) 1032
BssMI GATC 5 cut(s) 139, 237, 458, 889, 894
Bst4CI ACNGT 4 cut(s) 377, 626, 692, 702
Bst6I CTCTTC 4 cut(s) 243, 258, 276, 855
BstAFI CTTAAG 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 784, 788
BstDEI CTNAG 5 cut(s) 327, 468, 534, 764, 971
BstF5I GGATG 7 cut(s) 322, 390, 408, 429, 1047, 1170, 1237
BstH2I RGCGCY 1 cut(s) 966
BstHHI GCGC 1 cut(s) 965
BstKTI GATC 5 cut(s) 142, 240, 461, 892, 897
BstMAI GTCTC 6 cut(s) 287, 500, 524, 572, 767, 1242
BstMBI GATC 5 cut(s) 139, 237, 458, 889, 894
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstSCI CCNGG 2 cut(s) 240, 813
BstSFI CTRYAG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 427, 559, 1198
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 458
BstYI RGATCY 1 cut(s) 458
BsuRI GGCC 3 cut(s) 128, 769, 1093
BtsCI GGATG 7 cut(s) 322, 390, 408, 429, 1047, 1170, 1237
BtsI GCAGTG 1 cut(s) 617
BtsIMutI CAGTG 5 cut(s) 617, 622, 697, 1025, 1131
Cac8I GCNNGC 2 cut(s) 784, 788
CciI TCATGA 1 cut(s) 568
CfoI GCGC 1 cut(s) 965
Cfr13I GGNCC 3 cut(s) 559, 817, 833
CviAII CATG 6 cut(s) 255, 569, 657, 874, 995, 1089
DdeI CTNAG 5 cut(s) 327, 468, 534, 764, 971
DpnI GATC 5 cut(s) 141, 239, 460, 891, 896
DpnII GATC 5 cut(s) 139, 237, 458, 889, 894
EaeI YGGCCR 1 cut(s) 1091
Eam1104I CTCTTC 4 cut(s) 243, 258, 276, 855
EarI CTCTTC 4 cut(s) 243, 258, 276, 855
Eco31I GGTCTC 2 cut(s) 767, 1242
Eco47I GGWCC 3 cut(s) 559, 817, 833
Eco47III AGCGCT 1 cut(s) 964
Eco57I CTGAAG 5 cut(s) 171, 282, 534, 959, 992
EcoO109I RGGNCCY 1 cut(s) 817
FaeI CATG 6 cut(s) 258, 572, 660, 877, 998, 1092
FaqI GGGAC 1 cut(s) 803
FatI CATG 6 cut(s) 254, 568, 656, 873, 994, 1088
FauNDI CATATG 1 cut(s) 586
FbaI TGATCA 1 cut(s) 894
Fnu4HI GCNGC 3 cut(s) 416, 548, 1187
FokI GGATG 7 cut(s) 329, 397, 415, 416, 1054, 1177, 1244
Fsp4HI GCNGC 3 cut(s) 416, 548, 1187
FspBI CTAG 1 cut(s) 1220
GlaI GCGC 1 cut(s) 964
GluI GCNGC 3 cut(s) 416, 548, 1187
GsuI CTGGAG 1 cut(s) 527
HaeII RGCGCY 1 cut(s) 966
HaeIII GGCC 3 cut(s) 128, 769, 1093
HapII CCGG 3 cut(s) 242, 814, 1162
HhaI GCGC 1 cut(s) 965
Hin1II CATG 6 cut(s) 258, 572, 660, 877, 998, 1092
Hin6I GCGC 1 cut(s) 963
HinP1I GCGC 1 cut(s) 963
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 6 cut(s) 251, 643, 863, 869, 877, 1015
HpaII CCGG 3 cut(s) 242, 814, 1162
HphI GGTGA 2 cut(s) 122, 444
Hpy188I TCNGA 2 cut(s) 868, 894
Hpy188III TCNNGA 5 cut(s) 193, 569, 898, 937, 1162
HpyAV CCTTC 3 cut(s) 192, 210, 419
HpyCH4III ACNGT 4 cut(s) 377, 626, 692, 702
HpyCH4V TGCA 3 cut(s) 464, 622, 1139
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 5 cut(s) 327, 468, 534, 764, 971
Hsp92II CATG 6 cut(s) 258, 572, 660, 877, 998, 1092
HspAI GCGC 1 cut(s) 963
KflI GGGWCCC 1 cut(s) 817
Kpn2I TCCGGA 1 cut(s) 1161
Ksp22I TGATCA 1 cut(s) 894
Kzo9I GATC 5 cut(s) 139, 237, 458, 889, 894
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 305
Lsp1109I GCAGC 3 cut(s) 427, 559, 1198
LweI GCATC 4 cut(s) 438, 473, 547, 1126
MaeI CTAG 1 cut(s) 1220
MalI GATC 5 cut(s) 141, 239, 460, 891, 896
MboI GATC 5 cut(s) 139, 237, 458, 889, 894
MflI RGATCY 1 cut(s) 458
MhlI GDGCHC 2 cut(s) 410, 697
MlsI TGGCCA 1 cut(s) 1093
MluCI AATT 6 cut(s) 209, 710, 774, 837, 1046, 1173
MluNI TGGCCA 1 cut(s) 1093
MlyI GAGTC 2 cut(s) 260, 872
MmeI TCCRAC 1 cut(s) 1058
Mox20I TGGCCA 1 cut(s) 1093
MroI TCCGGA 1 cut(s) 1161
MroXI GAANNNNTTC 1 cut(s) 1110
MscI TGGCCA 1 cut(s) 1093
MseI TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
Msp20I TGGCCA 1 cut(s) 1093
MspA1I CMGCKG 1 cut(s) 1117
MspCI CTTAAG 1 cut(s) 419
MspI CCGG 3 cut(s) 242, 814, 1162
MspR9I CCNGG 2 cut(s) 242, 815
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 2 cut(s) 242, 815
NdeI CATATG 1 cut(s) 586
NdeII GATC 5 cut(s) 139, 237, 458, 889, 894
NlaIII CATG 6 cut(s) 258, 572, 660, 877, 998, 1092
NlaIV GGNNCC 4 cut(s) 93, 561, 818, 819
PagI TCATGA 1 cut(s) 568
PciSI GCTCTTC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1110
PfeI GAWTC 4 cut(s) 643, 869, 877, 1015
PflMI CCANNNNNTGG 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 3 cut(s) 417, 549, 1188
PleI GAGTC 2 cut(s) 259, 871
PpsI GAGTC 2 cut(s) 259, 871
PpuMI RGGWCCY 1 cut(s) 817
PshBI ATTAAT 1 cut(s) 840
Psp5II RGGWCCY 1 cut(s) 817
PspN4I GGNNCC 4 cut(s) 93, 561, 818, 819
PspPI GGNCC 3 cut(s) 559, 817, 833
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 1 cut(s) 458
PvuII CAGCTG 1 cut(s) 1117
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
SatI GCNGC 3 cut(s) 416, 548, 1187
Sau3AI GATC 5 cut(s) 139, 237, 458, 889, 894
Sau96I GGNCC 3 cut(s) 559, 817, 833
SchI GAGTC 2 cut(s) 260, 872
ScrFI CCNGG 2 cut(s) 242, 815
SduI GDGCHC 2 cut(s) 410, 697
SfaNI GCATC 4 cut(s) 438, 473, 547, 1126
SfcI CTRYAG 1 cut(s) 144
SinI GGWCC 3 cut(s) 559, 817, 833
SmlI CTYRAG 2 cut(s) 419, 594
SmoI CTYRAG 2 cut(s) 419, 594
Sse9I AATT 6 cut(s) 209, 710, 774, 837, 1046, 1173
SspMI CTAG 1 cut(s) 1220
StyD4I CCNGG 2 cut(s) 240, 813
TaaI ACNGT 4 cut(s) 377, 626, 692, 702
TasI AATT 6 cut(s) 209, 710, 774, 837, 1046, 1173
TfiI GAWTC 4 cut(s) 643, 869, 877, 1015
Tru1I TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
Tru9I TTAA 7 cut(s) 18, 132, 203, 420, 840, 1049, 1276
TscAI CASTG 5 cut(s) 624, 629, 697, 1032, 1138
TseI GCWGC 3 cut(s) 415, 547, 1186
TspDTI ATGAA 5 cut(s) 129, 166, 759, 1058, 1226
TspGWI ACGGA 1 cut(s) 415
TspRI CASTG 5 cut(s) 624, 629, 697, 1032, 1138
Van91I CCANNNNNTGG 1 cut(s) 309
Vha464I CTTAAG 1 cut(s) 419
VpaK11BI GGWCC 3 cut(s) 559, 817, 833
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 1110
XspI CTAG 1 cut(s) 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.