MD09G1165600.v1.1

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011436
Physical Location & Seq
Forward (+)
13543766 .. 13547950
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1165600.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGGATTTCTTCAAAGTTAAGAAGTTTAGGAAAGCACACAAACCATCCCCGGAAAATGTGTCAGAGGATAATCCTGTGCCACAGCCGGAGGAACCCGGCAAGGAGAGTAGTGGTGATGATTTTGGTAAGTCGGCCAATGCTGATTCTGTGGCAGAAGCTGAGGACGATGATGATGATTTCATAACGGAGGAGGTCAAGCGGAGGTTAAAAGAATTGAGAAGGAATAGCTTCATGGTGTTGATTCCGGAAGAAGAGTCATTTCCCGAAGATGAAGAAGAAGAAGAAGAGCAGGCCGGTGAAACAAGCTCTAACGAGTGGAGGGATGTAGAGGCAGAAGGCCGGCAATGGTGGTGTGGCTTTGGTGCTGTATATGACAAGTACTGGGAGAGGATGTTGTTCTTTGAACGGATGAGTGCGCAGCATCTTAATGGAACTGGCATCCAAACCCCTATGACCCCATCACCGCGATCAGCATCTAAGAAGCTTACCTCCCCTTTTCGCTGTCTTTCTTTGAAGAAGATTGAAGAGCCTGATGATGAAACTGAGCACCTTCAGCAGCCGGAAAATGATCTGTACCAGGATATCGAGACATCATATGTAGGTCAACTTTGCTTGTCTTGGGAGGCACTGCACTGTCAGTACACTCAACTAAACCAGCTAATCGCTTGCCAACCTGAAAATCCCAATAGTTACAACCATAGTGCTCAACAGTTTCAACAGTTCCAGGTTTTACTACAAAGGTATATCGAAAATGAACCTTTTGAGGAGGGCCACAGGGCTGAAATTTATGCTCGCACACAAAGACTTCTTCCTAATTTACTGCGGGTGCCTAATATACAAGGTCCAATTCAAAAAGGAAATGAGGAAGAATTAGATTCGGTGGTGCATGCTCCTGACCTCATCAAGATTATTGAAACCTCAATCCTCACTTTCCAGCTTTTCCTGAAGATGGATAAGAAAAAACCAAGCTCTGTTCTTAACTTGTTTGGGAATCAAAACCAGGCTGCCACCCCACTTCAGCAGATTCAATCTTCTCTCGAGAAGAAATGGATGAAGTTAAAGGAACTGCAAAAAAGGAGGAAAGGTTGGAAGAAGAAATCGTGGCCGCAGTTGCAAGAAGATGTTCAGCTTTTGTTTAGCCTCATTGACGCCAAGGTTCTGTCGAGAACCCTCCGTATGGTGAGACTAAGCAAAGAGCAGCTGTTTTGGTGCGAGGAGAAGATGAAAAAACTCGATTTGGTAGATGGAAAGTTGTGGAGAGACCCGTCTCCCACCCTTTTCCCTTGTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

431

Amino Acids

50.17

Weight (kDa)

5.11

Isoelectric Point (pI)

62.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 193 - 428 9.6e-90 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011436)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20260
fragaria_vesca FvH4_6g36930 FvH4_6g36930 FvH4_6g36930
malus_domestica MD09G1165600.v1.1 MD17G1162200.v1.1
prunus_persica Prupe.3G023200_v2.0.a1
pyrus_communis pycom09g08290 pycom17g15530
rosa_chinensis RchiOBHm_Chr2g0149351
rosa_laevigata RLG00000020395
rosa_roxburghii Rroxscaffold_2G00098170
rosa_rugosa Rorug02G0411600 Rorug02G0411700 Rorug02G0411800
rosa_samantha Rh2AG471500 Rh2BG483800 Rh2CG457500 Rh2DG492800
rosa_wichuraiana Rw2G038370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 826
AccII CGCG 1 cut(s) 466
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 4 cut(s) 199, 464, 823, 1106
AcoI YGGCCR 2 cut(s) 132, 1103
AcsI RAATTY 1 cut(s) 783
AcuI CTGAAG 3 cut(s) 536, 965, 1001
AcyI GRCGYC 1 cut(s) 1149
AfaI GTAC 3 cut(s) 380, 575, 641
AfiI CCNNNNNNNGG 2 cut(s) 949, 1177
AgsI TTSAA 8 cut(s) 13, 404, 514, 524, 716, 851, 914, 1028
AjnI CCWGG 3 cut(s) 576, 723, 999
AluBI AGCT 9 cut(s) 158, 228, 306, 484, 658, 937, 969, 1129, 1201
AluI AGCT 9 cut(s) 158, 228, 306, 484, 658, 937, 969, 1129, 1201
Alw21I GWGCWC 2 cut(s) 549, 706
Alw26I GTCTC 4 cut(s) 581, 1177, 1254, 1272
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 1037
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 5 cut(s) 132, 291, 337, 769, 1103
ApeKI GCWGC 4 cut(s) 418, 556, 1004, 1198
ApoI RAATTY 1 cut(s) 783
Asp700I GAANNNNTTC 2 cut(s) 227, 1122
AspLEI GCGC 1 cut(s) 418
AspS9I GGNCC 2 cut(s) 769, 842
AsuC2I CCSGG 2 cut(s) 50, 96
AsuHPI GGTGA 4 cut(s) 125, 308, 453, 1192
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 1 cut(s) 842
BanI GGYRCC 1 cut(s) 826
Bbv12I GWGCWC 2 cut(s) 549, 706
BbvCI CCTCAGC 1 cut(s) 159
BbvI GCAGC 4 cut(s) 430, 568, 991, 1210
BccI CCATC 4 cut(s) 52, 466, 943, 1238
BciT130I CCWGG 3 cut(s) 578, 725, 1001
BcnI CCSGG 2 cut(s) 50, 96
BcoDI GTCTC 4 cut(s) 581, 1177, 1254, 1272
BisI GCNGC 5 cut(s) 419, 557, 1005, 1106, 1199
BlsI GCNGC 5 cut(s) 420, 558, 1006, 1107, 1200
BmcAI AGTACT 1 cut(s) 380
Bme1390I CCNGG 5 cut(s) 50, 96, 578, 725, 1001
Bme18I GGWCC 1 cut(s) 842
BmeT110I CYCGRG 1 cut(s) 1037
BmgT120I GGNCC 2 cut(s) 769, 842
BmiI GGNNCC 2 cut(s) 93, 828
BmrFI CCNGG 5 cut(s) 50, 96, 578, 725, 1001
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 430, 447, 482
BmuI ACTGGG 1 cut(s) 391
Bpu10I CCTNAGC 1 cut(s) 159
BpuMI CCSGG 2 cut(s) 50, 96
BsaBI GATNNNNATC 1 cut(s) 472
BsaHI GRCGYC 1 cut(s) 1149
BsaI GGTCTC 1 cut(s) 1254
BsaJI CCNNGG 2 cut(s) 48, 1152
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 4 cut(s) 377, 407, 1069, 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 949, 1177
Bse118I RCCGGY 2 cut(s) 293, 339
Bse1I ACTGG 2 cut(s) 386, 439
Bse3DI GCAATG 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 472
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 3 cut(s) 578, 725, 1001
BseDI CCNNGG 2 cut(s) 48, 1152
BseGI GGATG 6 cut(s) 44, 328, 396, 414, 438, 1056
BseJI GATNNNNATC 1 cut(s) 472
BseLI CCNNNNNNNGG 2 cut(s) 949, 1177
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 2 cut(s) 150, 534
BseNI ACTGG 2 cut(s) 386, 439
BseRI GAGGAG 3 cut(s) 203, 779, 1229
BseXI GCAGC 4 cut(s) 430, 568, 991, 1210
BsgI GTGCAG 1 cut(s) 614
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 5 cut(s) 134, 293, 339, 771, 1105
BshNI GGYRCC 1 cut(s) 826
BsiHKAI GWGCWC 2 cut(s) 549, 706
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 7 cut(s) 50, 86, 96, 245, 294, 340, 560
BslI CCNNNNNNNGG 2 cut(s) 949, 1177
BsmAI GTCTC 4 cut(s) 581, 1177, 1254, 1272
BsmBI CGTCTC 1 cut(s) 1272
BsnI GGCC 5 cut(s) 134, 293, 339, 771, 1105
Bso31I GGTCTC 1 cut(s) 1254
BsoBI CYCGRG 1 cut(s) 1037
Bsp1286I GDGCHC 2 cut(s) 549, 706
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 2 cut(s) 467, 568
BspACI CCGC 4 cut(s) 199, 464, 823, 1106
BspANI GGCC 5 cut(s) 134, 293, 339, 771, 1105
BspCNI CTCAG 2 cut(s) 151, 535
BspEI TCCGGA 1 cut(s) 244
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 93, 828
BspQI GCTCTTC 2 cut(s) 279, 519
BspT107I GGYRCC 1 cut(s) 826
BspTNI GGTCTC 1 cut(s) 1254
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 2 cut(s) 293, 339
BsrI ACTGG 2 cut(s) 386, 439
BssAI RCCGGY 2 cut(s) 293, 339
BssECI CCNNGG 2 cut(s) 48, 1152
BssMI GATC 2 cut(s) 467, 568
BssNI GRCGYC 1 cut(s) 1149
BssT1I CCWWGG 1 cut(s) 1152
Bst2UI CCWGG 3 cut(s) 578, 725, 1001
Bst4CI ACNGT 3 cut(s) 635, 711, 720
Bst6I CTCTTC 3 cut(s) 246, 279, 519
BstACI GRCGYC 1 cut(s) 1149
BstC8I GCNNGC 5 cut(s) 291, 341, 667, 793, 888
BstDEI CTNAG 4 cut(s) 159, 477, 543, 1187
BstF5I GGATG 6 cut(s) 44, 328, 396, 414, 438, 1056
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 1 cut(s) 418
BstKTI GATC 2 cut(s) 470, 571
BstMAI GTCTC 4 cut(s) 581, 1177, 1254, 1272
BstMBI GATC 2 cut(s) 467, 568
BstMWI GCNNNNNNNGC 2 cut(s) 553, 1111
BstNI CCWGG 3 cut(s) 578, 725, 1001
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 5 cut(s) 48, 94, 576, 723, 999
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 4 cut(s) 430, 568, 991, 1210
BsuRI GGCC 5 cut(s) 134, 293, 339, 771, 1105
BtsCI GGATG 6 cut(s) 44, 328, 396, 414, 438, 1056
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 2 cut(s) 626, 631
Cac8I GCNNGC 5 cut(s) 291, 341, 667, 793, 888
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 418
Cfr10I RCCGGY 2 cut(s) 293, 339
Cfr13I GGNCC 2 cut(s) 769, 842
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 3 cut(s) 379, 574, 640
CviAII CATG 2 cut(s) 232, 887
CviQI GTAC 3 cut(s) 379, 574, 640
DdeI CTNAG 4 cut(s) 159, 477, 543, 1187
DpnI GATC 2 cut(s) 469, 570
DpnII GATC 2 cut(s) 467, 568
EaeI YGGCCR 2 cut(s) 132, 1103
Eam1104I CTCTTC 3 cut(s) 246, 279, 519
EarI CTCTTC 3 cut(s) 246, 279, 519
Eco130I CCWWGG 1 cut(s) 1152
Eco31I GGTCTC 1 cut(s) 1254
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 842
Eco57I CTGAAG 3 cut(s) 536, 965, 1001
Eco88I CYCGRG 1 cut(s) 1037
EcoRII CCWGG 3 cut(s) 576, 723, 999
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 1152
Esp3I CGTCTC 1 cut(s) 1272
FaeI CATG 2 cut(s) 235, 890
FatI CATG 2 cut(s) 231, 886
FauI CCCGC 1 cut(s) 816
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 5 cut(s) 419, 557, 1005, 1106, 1199
FokI GGATG 6 cut(s) 31, 335, 403, 421, 425, 1063
Fsp4HI GCNGC 5 cut(s) 419, 557, 1005, 1106, 1199
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 1 cut(s) 417
GluI GCNGC 5 cut(s) 419, 557, 1005, 1106, 1199
HaeIII GGCC 5 cut(s) 134, 293, 339, 771, 1105
HapII CCGG 7 cut(s) 50, 86, 96, 245, 294, 340, 560
HgaI GACGC 1 cut(s) 1157
HhaI GCGC 1 cut(s) 418
Hin1I GRCGYC 1 cut(s) 1149
Hin1II CATG 2 cut(s) 235, 890
Hin6I GCGC 1 cut(s) 416
HinP1I GCGC 1 cut(s) 416
HincII GTYRAC 1 cut(s) 605
HindII GTYRAC 1 cut(s) 605
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 6 cut(s) 143, 241, 254, 875, 991, 1024
HpaII CCGG 7 cut(s) 50, 86, 96, 245, 294, 340, 560
HphI GGTGA 4 cut(s) 125, 308, 453, 1192
Hpy166II GTNNAC 2 cut(s) 605, 642
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 9 cut(s) 245, 263, 586, 893, 904, 943, 1037, 1039, 1164
Hpy8I GTNNAC 2 cut(s) 605, 642
HpyAV CCTTC 3 cut(s) 213, 329, 560
HpyCH4III ACNGT 3 cut(s) 635, 711, 720
HpyCH4V TGCA 4 cut(s) 631, 886, 1069, 1114
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 1111
HpyF3I CTNAG 4 cut(s) 159, 477, 543, 1187
Hsp92I GRCGYC 1 cut(s) 1149
Hsp92II CATG 2 cut(s) 235, 890
HspAI GCGC 1 cut(s) 416
Kpn2I TCCGGA 1 cut(s) 244
KroI GCCGGC 1 cut(s) 339
KroNI GCCGGC 1 cut(s) 341
Kzo9I GATC 2 cut(s) 467, 568
LguI GCTCTTC 2 cut(s) 279, 519
LmnI GCTCC 1 cut(s) 895
Lsp1109I GCAGC 4 cut(s) 430, 568, 991, 1210
LweI GCATC 3 cut(s) 430, 447, 482
MaeIII GTNAC 1 cut(s) 689
MalI GATC 2 cut(s) 469, 570
MboI GATC 2 cut(s) 467, 568
MhlI GDGCHC 2 cut(s) 549, 706
MluCI AATT 5 cut(s) 212, 783, 814, 846, 869
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 1067
MroI TCCGGA 1 cut(s) 244
MroNI GCCGGC 1 cut(s) 339
MroXI GAANNNNTTC 2 cut(s) 227, 1122
MseI TTAA 5 cut(s) 18, 206, 426, 978, 1058
MslI CAYNNNNRTG 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 1201
MspI CCGG 7 cut(s) 50, 86, 96, 245, 294, 340, 560
MspR9I CCNGG 5 cut(s) 50, 96, 578, 725, 1001
MvaI CCWGG 3 cut(s) 578, 725, 1001
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 2 cut(s) 553, 1111
NaeI GCCGGC 1 cut(s) 341
NciI CCSGG 2 cut(s) 50, 96
NdeI CATATG 1 cut(s) 595
NdeII GATC 2 cut(s) 467, 568
NgoMIV GCCGGC 1 cut(s) 339
NlaIII CATG 2 cut(s) 235, 890
NlaIV GGNNCC 2 cut(s) 93, 828
NsbI TGCGCA 1 cut(s) 417
NspI RCATGY 1 cut(s) 890
PaeI GCATGC 1 cut(s) 890
PaeR7I CTCGAG 1 cut(s) 1037
PciSI GCTCTTC 2 cut(s) 279, 519
PdiI GCCGGC 1 cut(s) 341
PdmI GAANNNNTTC 2 cut(s) 227, 1122
PfeI GAWTC 5 cut(s) 143, 241, 875, 991, 1024
PkrI GCNGC 5 cut(s) 420, 558, 1006, 1107, 1200
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp6I CCWGG 3 cut(s) 576, 723, 999
PspGI CCWGG 3 cut(s) 576, 723, 999
PspN4I GGNNCC 2 cut(s) 93, 828
PspPI GGNCC 2 cut(s) 769, 842
PstNI CAGNNNCTG 1 cut(s) 158
PvuII CAGCTG 1 cut(s) 1201
RsaI GTAC 3 cut(s) 380, 575, 641
RsaNI GTAC 3 cut(s) 379, 574, 640
RseI CAYNNNNRTG 1 cut(s) 426
SapI GCTCTTC 2 cut(s) 279, 519
SaqAI TTAA 5 cut(s) 18, 206, 426, 978, 1058
SatI GCNGC 5 cut(s) 419, 557, 1005, 1106, 1199
Sau3AI GATC 2 cut(s) 467, 568
Sau96I GGNCC 2 cut(s) 769, 842
ScaI AGTACT 1 cut(s) 380
SchI GAGTC 1 cut(s) 263
ScrFI CCNGG 5 cut(s) 50, 96, 578, 725, 1001
SduI GDGCHC 2 cut(s) 549, 706
SfaNI GCATC 3 cut(s) 430, 447, 482
Sfr274I CTCGAG 1 cut(s) 1037
SinI GGWCC 1 cut(s) 842
SlaI CTCGAG 1 cut(s) 1037
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
SphI GCATGC 1 cut(s) 890
Sse9I AATT 5 cut(s) 212, 783, 814, 846, 869
SsiI CCGC 4 cut(s) 199, 464, 823, 1106
StyD4I CCNGG 5 cut(s) 48, 94, 576, 723, 999
StyI CCWWGG 1 cut(s) 1152
TaaI ACNGT 3 cut(s) 635, 711, 720
TaqI TCGA 5 cut(s) 585, 747, 1038, 1163, 1233
TasI AATT 5 cut(s) 212, 783, 814, 846, 869
TatI WGTACW 2 cut(s) 378, 639
TauI GCSGC 1 cut(s) 1108
TfiI GAWTC 5 cut(s) 143, 241, 875, 991, 1024
Tru1I TTAA 5 cut(s) 18, 206, 426, 978, 1058
Tru9I TTAA 5 cut(s) 18, 206, 426, 978, 1058
TscAI CASTG 2 cut(s) 633, 638
TseI GCWGC 4 cut(s) 418, 556, 1004, 1198
TspDTI ATGAA 7 cut(s) 169, 220, 285, 552, 768, 1067, 1238
TspGWI ACGGA 3 cut(s) 200, 421, 1163
TspRI CASTG 2 cut(s) 633, 638
VpaK11BI GGWCC 1 cut(s) 842
XapI RAATTY 1 cut(s) 783
XceI RCATGY 1 cut(s) 890
XhoI CTCGAG 1 cut(s) 1037
XmnI GAANNNNTTC 2 cut(s) 227, 1122
ZrmI AGTACT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.