Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011243

Physical Location & Seq

Genomic Coordinates Forward (+)

7168054 .. 7173617

Gene Structure 5564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G20540.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3105 bp

ATGGGGGTTTCTCTTCGTCACTTGTCTCCTTCCTCTTTCTGGGTTTCGCGTCGTCCTCGTGTTTCTTCTTCAATTCTTTCTTTTCTTGTCCCTCGCCGTCGAATCCTTTGCACCAGGAAAGTAGCAATCATAAAGGGAAATGCGGGATATTCGACTGCTACTGATTGTGGTGGTTCTCATGGGTTTCATCATTCAGGTCATCAAAGGTCATCCTCTGTTGAGTTTAGTGGAGAGTGGAAACTTAATTTGGGATCTAAGACGGCGAGAATGGTTCCGCCAACTGTGAAACAAGCTGGAGCTGTAAGTGCTTGGAGGGAGGAAGTCAATAATAAGTTAAGAGGAAGAAATAGAGAATATGCTAACAACCAGGATGATGCTTTTGGTAATGGCAGTTATATTTTAAAAGGCTTTGTCCCCAAAATAGATGATGTCCATTCTTATGGAAACGGCCAGAACTTTGATTACAATCTGAAGCCTGGAACTGATATTACCACACTTGGTAGAGAATTGAATGGCTTTATGCAAACGAATAGTATAAGAGGATCGGTCGTAGCATTACCAAGCAAGGATATTGAGGTTGGGGAAACAACAGACGTGACTCTGAAACCTCTTAACAGTGATACGACTCTTGACAATGCCAGCTATAAGAAAACAGCAACCATTTCCAAAGTGGAAAAATGCACCAACCTATCGCAAGTCCGTGCAAACCTCAAGAAAATATACAATAGAGTTCGTGTTGTTGATAATGTGTCTAGTGCAAAGGAAACCGTGGCTCTGCTCATGAATCAATATAGGAATCTTGTCCATGCTTGCGATACAGAGGTATCCAGGATTGATGTGAAGACTGAAACACCTGTAGACCATGGGGAGATGATTTGTTTCAGTATCTATTGTGGATCAGAAGCAGATTTTGGAGATGGTAAATCATGTATCTGGGTAGATGTGCTTGGCGAAAATGGAAGGGATATCCTGGCTGAGTTTAAGCCATTTTTCGAAGACTCATCCATAAAAAAAGTATGGCATAACTACAGCTTTGATAACCACATCATCAGAAATTATGGAATCAAGCTTTCTGGTTTTCATGGTGATACAATGCACATGGCACGATTGTGGGATTCATCTAGACGGATATCGGGTGGTTATTCGCTTGAAGCACTTACAAGTGACCCAAAAGTTCTTGGGGGAACTGAGACAAAGGAGGAAGCAGAATTATTTGGTAAAATATCAATGAAGAAGATTTTCGGCAAGGGAAAATTGAAAAAAGATGGATCAGAAGGAAAATTGGTGATCATTCCTCCTGTTAAAGAGCTACAAATGGAGGATCGAGAAGCTTGGATTTCGTACTCAGCGTTGGATTCAATAAGCACTCTAAAGCTTTATGAGAGCATGAAGAAGCAACTGCAAGCGAAGAAATGGTTTCTTGATGGAAAACTAATTTCAAAAAAGAACATGTTTGATTTCTACCAAGAATATTGGCAACCTTTTGGTGAACTTCTTGCCAAAATGGAATCGGAAGGAATGCTTGTAGATAGGGATTATTTGGCGCAGATTGAGATTGTAGCCAAAGCAGAACAAGAAATTGCTGTTTCGAGGTTCCGTAATTGGGCTTCAAAGCATTGTCCAGATGCAAAGCATATGAATGTTGGCAGTGACACGCAATTGCGACAACTCTTTTTTGGTGGCATTTCTAACAGTTGTAATGATGAGGATCTTCCATATGAAAAACTTTTCAAAGTTCCCAATGTGGATAAGGTGATTGAAGAAGGAAAAAAGAGAGCCACAAAATTCCGGAATATCAAACTGCATAGGATAAGCGACAGACCTTTGCCCACTGAAAAGTTCACTGCCTCAGGCTGGCCCTCTGTTAGTGGAGACACCTTGAAAGCCTTAGCTGGGAAAGTCTCTGCAGAATATGACTATATGGAGGGCGTTTTAGATACTTGTCTAGAAGAAAACATTGGAGATGATGATTGTATTTCGCTACCAGATGAAGTTGTAGAAACACAACACGTCAATACCTCTGTTGAATCAGACACATCTGCTTATGGAACAGCATTTGATGCATTTGGAGGGGGTGAAAGTGGAAAGGAAGCTTGCCATGCTATTGCTGCATTATGTGAAGTTTGCTCCATTGATTCCTTAATATCAAATTTTATCCTTCCTTTACAGGGAAGTAACGTGTCAGGAAAAGATGGTCGTGTCCACTGCTCCCTGAATATCAACACTGAAACTGGACGCTTATCGGCTAGAAGGCCAAATTTGCAGAACCAACCTGCATTGGAGAAAGATCGGTACAAAATCCGTCAGGCCTTCATAGCATCACCTGGAAATTCACTTATTGTTGCTGATTATGGACAGCTGGAACTTAGGATTCTGGCACATCTTGCTAGTTGCGAAAGCATGAAGGAAGCTTTCATAGCTGGTGGAGACTTCCACTCAAGAACAGCCATGAATATGTATCCTCATATTCGTGAAGCTGTTGAAAATGGTGAAGTGCTCCTTGAATGGCATCCACAACCTGGACAAGAGAAGCCGCCAGTGCCATTGTTGAAGGATGCCTTTGCTTCTGAGAGAAGAAAAGCAAAGATGCTTAACTTCTCGATTGCGTATGGGAAGACTGCTATTGGGCTGTCTAGAGATTGGAAGGTATCAAGAGAAGAAGCTCAAGATACAGTTAATCTCTGGTATAATGACAGACAAGAAGTCCGGAAATGGCAAGAACTACGCAAGAAAGAAGCTATACAAAAAGGGTATGTACTCACTTTGTTGGGAAGGGCTCGTAAATTCCCTGAATACCGTTCACGTGCCCAAAAGAACCATATCGAGCGAGCAGCAATCAACACTCCTGTTCAGGGAAGTGCGGCTGATGTTGCTATGTGCGCTATGCTGGAGATATCAAATAATCAACGGCTAAAAGAGCTTGGTTGGAAATTGCTTCTACAAGTTCATGATGAAGTAATCTTGGAAGGACCAAGTGAGTCAGCGGAGAACGCTAAAGACATAGTTGTGAACTGCATGTCTGAACCCTTCAACGGCAAGAATATTCTCTCAGTCGACTTATCTGTTGATGCTAAGTGTGCTCAGAACTGGTATGCTGGCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0003674 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006264 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009657 GO:0009987 GO:0016043 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0032042 GO:0033258 GO:0033259 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03440 map00230 map00240 map01100 map03030 map03410 map03420 map03440

Pfam Domains

Protein Families

DNA_pol_A DNA_pol_A_exo1

Protein Analysis

1034

Amino Acids

115.56

Weight (kDa)

7.93

Isoelectric Point (pI)

37.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_A_exo1	PF01612	316 - 483	1e-10	3'-5' exonuclease
DNA_pol_A	PF00476	716 - 842	1.1e-28	DNA polymerase family A
DNA_pol_A	PF00476	870 - 1046	1.6e-45	DNA polymerase family A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50840 AT1G50840 AT3G20540 AT3G20540
fragaria_vesca	FvH4_3g22230
malus_domestica	MD11G1221900.v1.1
prunus_persica	Prupe.4G209500_v2.0.a1 Prupe.4G209500_v2.0.a1 Prupe.4G209500_v2.0.a1
pyrus_communis	pycom11g19570
rosa_chinensis	RchiOBHm_Chr5g0038301
rosa_laevigata	RLG00000033845
rosa_multiflora	Rmu_co8325491.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042720
rosa_rugosa	Rorug05G0171900
rosa_samantha	Rh5AG259200 Rh5BG263000 Rh5CG295400 Rh5DG272000
rosa_wichuraiana	Rw5G024260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2281
AccB7I	CCANNNNNTGG	1 cut(s)	2519
AccI	GTMKAC	2 cut(s)	858, 3054
AccII	CGCG	1 cut(s)	49
AccIII	TCCGGA	2 cut(s)	1788, 2706
AciI	CCGC	5 cut(s)	143, 275, 2534, 2861, 2984
AclWI	GGATC	6 cut(s)	259, 550, 904, 1276, 1329, 1716
AcoI	YGGCCR	1 cut(s)	448
AcsI	RAATTY	5 cut(s)	1784, 2149, 2257, 2329, 2783
AcuI	CTGAAG	1 cut(s)	491
AcvI	CACGTG	1 cut(s)	2804
AfaI	GTAC	3 cut(s)	1343, 2294, 2757
AfiI	CCNNNNNNNGG	8 cut(s)	39, 1603, 1854, 1893, 2168, 2519, 2852, 3032
AflIII	ACRYGT	3 cut(s)	1449, 2008, 2178
AhdI	GACNNNNNGTC	1 cut(s)	2702
AjiI	CACGTC	2 cut(s)	595, 2011
AjnI	CCWGG	7 cut(s)	113, 366, 475, 827, 969, 2323, 2518
AjuI	GAANNNNNNNTTGG	6 cut(s)	230, 262, 894, 926, 1941, 1973
AleI	CACNNNNGTG	1 cut(s)	1108
Alw21I	GWGCWC	2 cut(s)	2499, 3082
Alw26I	GTCTC	5 cut(s)	30, 1184, 1866, 1906, 2421
AlwI	GGATC	6 cut(s)	259, 550, 904, 1276, 1329, 1716
Aor13HI	TCCGGA	2 cut(s)	1788, 2706
AoxI	GGCC	4 cut(s)	448, 1856, 2252, 2307
ApeKI	GCWGC	2 cut(s)	2108, 2831
ApoI	RAATTY	5 cut(s)	1784, 2149, 2257, 2329, 2783
Asp700I	GAANNNNTTC	1 cut(s)	1238
AspLEI	GCGC	2 cut(s)	1546, 2882
AspS9I	GGNCC	2 cut(s)	1857, 2969
AsuHPI	GGTGA	7 cut(s)	1097, 1297, 1499, 1765, 2087, 2313, 2501
AsuII	TTCGAA	1 cut(s)	993
AvaII	GGWCC	1 cut(s)	2969
AxyI	CCTNAGG	1 cut(s)	1849
BaeGI	GKGCMC	1 cut(s)	2809
BaeI	ACNNNNGTAYC	4 cut(s)	807, 807, 840, 840
BanII	GRGCYC	1 cut(s)	2779
BarI	GAAGNNNNNNTAC	2 cut(s)	1591, 1623
BauI	CACGAG	1 cut(s)	57
BbrPI	CACGTG	1 cut(s)	2804
BbsI	GAAGAC	3 cut(s)	848, 1002, 2621
Bbv12I	GWGCWC	2 cut(s)	2499, 3082
BbvI	GCAGC	2 cut(s)	2095, 2843
BccI	CCATC	4 cut(s)	911, 1259, 1418, 2186
BceAI	ACGGC	5 cut(s)	81, 276, 463, 2924, 3049
BcgI	CGANNNNNNTGC	2 cut(s)	90, 124
BciT130I	CCWGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
BciVI	GTATCC	2 cut(s)	835, 2469
BclI	TGATCA	1 cut(s)	1287
BcoDI	GTCTC	5 cut(s)	30, 1184, 1866, 1906, 2421
BfaI	CTAG	6 cut(s)	753, 1122, 1946, 2247, 2388, 2634
BfmI	CTRYAG	3 cut(s)	855, 1027, 1905
BfuAI	ACCTGC	1 cut(s)	2281
BfuI	GTATCC	2 cut(s)	835, 2469
BisI	GCNGC	4 cut(s)	2109, 2534, 2832, 2862
BlsI	GCNGC	4 cut(s)	2110, 2535, 2833, 2863
Bme1390I	CCNGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
Bme18I	GGWCC	1 cut(s)	2969
BmeRI	GACNNNNNGTC	1 cut(s)	2702
BmgBI	CACGTC	2 cut(s)	595, 2011
BmgT120I	GGNCC	2 cut(s)	1857, 2969
BmiI	GGNNCC	2 cut(s)	273, 1595
BmrFI	CCNGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
BmsI	GCATC	8 cut(s)	364, 1615, 2050, 2327, 2518, 2545, 2577, 3058
BpiI	GAAGAC	3 cut(s)	848, 1002, 2621
BpmI	CTGGAG	2 cut(s)	315, 2909
Bpu10I	CCTNAGC	1 cut(s)	1888
Bpu14I	TTCGAA	1 cut(s)	993
BpuEI	CTTGAG	3 cut(s)	695, 2422, 2649
BsaAI	YACGTR	1 cut(s)	2804
BsaBI	GATNNNNATC	2 cut(s)	465, 1707
BsaJI	CCNNGG	2 cut(s)	768, 862
BsaWI	WCCGGW	2 cut(s)	1788, 2706
Bsc4I	CCNNNNNNNGG	8 cut(s)	39, 1603, 1854, 1893, 2168, 2519, 2852, 3032
Bse1I	ACTGG	3 cut(s)	2236, 2537, 3092
Bse21I	CCTNAGG	1 cut(s)	1849
Bse8I	GATNNNNATC	2 cut(s)	465, 1707
BseAI	TCCGGA	2 cut(s)	1788, 2706
BseBI	CCWGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
BseDI	CCNNGG	2 cut(s)	768, 862
BseGI	GGATG	5 cut(s)	209, 376, 1001, 2509, 2560
BseJI	GATNNNNATC	2 cut(s)	465, 1707
BseLI	CCNNNNNNNGG	8 cut(s)	39, 1603, 1854, 1893, 2168, 2519, 2852, 3032
BseMII	CTCAG	7 cut(s)	966, 1179, 1359, 1863, 2559, 3063, 3095
BseNI	ACTGG	3 cut(s)	2236, 2537, 3092
BseSI	GKGCMC	1 cut(s)	2809
BseXI	GCAGC	2 cut(s)	2095, 2843
BseYI	CCCAGC	1 cut(s)	1892
Bsh1236I	CGCG	1 cut(s)	49
Bsh1285I	CGRYCG	1 cut(s)	549
BshFI	GGCC	4 cut(s)	450, 1858, 2254, 2309
BsiEI	CGRYCG	1 cut(s)	549
BsiHKAI	GWGCWC	2 cut(s)	2499, 3082
BsiSI	CCGG	2 cut(s)	1789, 2707
BslFI	GGGAC	2 cut(s)	74, 398
BslI	CCNNNNNNNGG	8 cut(s)	39, 1603, 1854, 1893, 2168, 2519, 2852, 3032
BsmAI	GTCTC	5 cut(s)	30, 1184, 1866, 1906, 2421
BsmFI	GGGAC	2 cut(s)	74, 398
BsmI	GAATGC	1 cut(s)	1524
BsnI	GGCC	4 cut(s)	450, 1858, 2254, 2309
Bsp119I	TTCGAA	1 cut(s)	993
Bsp1286I	GDGCHC	4 cut(s)	2499, 2779, 2809, 3082
Bsp13I	TCCGGA	2 cut(s)	1788, 2706
Bsp143I	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
Bsp19I	CCATGG	1 cut(s)	862
BspACI	CCGC	5 cut(s)	143, 275, 2534, 2861, 2984
BspANI	GGCC	4 cut(s)	450, 1858, 2254, 2309
BspCNI	CTCAG	7 cut(s)	967, 1180, 1358, 1862, 2560, 3062, 3094
BspEI	TCCGGA	2 cut(s)	1788, 2706
BspFNI	CGCG	1 cut(s)	49
BspHI	TCATGA	2 cut(s)	780, 2947
BspLI	GGNNCC	2 cut(s)	273, 1595
BspMAI	CTGCAG	1 cut(s)	1909
BspMI	ACCTGC	1 cut(s)	2281
BspPI	GGATC	6 cut(s)	259, 550, 904, 1276, 1329, 1716
BspT104I	TTCGAA	1 cut(s)	993
BsrI	ACTGG	3 cut(s)	2236, 2537, 3092
BssECI	CCNNGG	2 cut(s)	768, 862
BssMI	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
BssSI	CACGAG	1 cut(s)	57
BssT1I	CCWWGG	1 cut(s)	862
Bst2BI	CACGAG	1 cut(s)	57
Bst2UI	CCWGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
Bst4CI	ACNGT	6 cut(s)	283, 617, 769, 1694, 2674, 2798
Bst6I	CTCTTC	1 cut(s)	18
BstAPI	GCANNNNNTGC	2 cut(s)	2060, 2384
BstBAI	YACGTR	1 cut(s)	2804
BstBI	TTCGAA	1 cut(s)	993
BstC8I	GCNNGC	7 cut(s)	640, 811, 1404, 1856, 2095, 2829, 3097
BstDSI	CCRYGG	2 cut(s)	768, 862
BstF5I	GGATG	5 cut(s)	209, 376, 1001, 2509, 2560
BstFNI	CGCG	1 cut(s)	49
BstHHI	GCGC	2 cut(s)	1546, 2882
BstKTI	GATC	8 cut(s)	254, 545, 899, 1271, 1290, 1324, 1711, 2290
BstMAI	GTCTC	5 cut(s)	30, 1184, 1866, 1906, 2421
BstMBI	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
BstMCI	CGRYCG	1 cut(s)	549
BstNI	CCWGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
BstNSI	RCATGY	2 cut(s)	1453, 3019
BstSCI	CCNGG	7 cut(s)	113, 366, 475, 827, 969, 2323, 2518
BstSFI	CTRYAG	3 cut(s)	855, 1027, 1905
BstSLI	GKGCMC	1 cut(s)	2809
BstUI	CGCG	1 cut(s)	49
BstV1I	GCAGC	2 cut(s)	2095, 2843
BstV2I	GAAGAC	3 cut(s)	848, 1002, 2621
BstX2I	RGATCY	2 cut(s)	251, 1708
BstXI	CCANNNNNNTGG	1 cut(s)	440
BstYI	RGATCY	2 cut(s)	251, 1708
Bsu36I	CCTNAGG	1 cut(s)	1849
BsuI	GTATCC	2 cut(s)	835, 2469
BsuRI	GGCC	4 cut(s)	450, 1858, 2254, 2309
BtgI	CCRYGG	2 cut(s)	768, 862
BtrI	CACGTC	2 cut(s)	595, 2011
BtsCI	GGATG	5 cut(s)	209, 376, 1001, 2509, 2560
BtsI	GCAGTG	3 cut(s)	1654, 1842, 2203
BtsIMutI	CAGTG	7 cut(s)	622, 1654, 1830, 1842, 2203, 2223, 2544
BveI	ACCTGC	1 cut(s)	2281
Cac8I	GCNNGC	7 cut(s)	640, 811, 1404, 1856, 2095, 2829, 3097
CciI	TCATGA	2 cut(s)	780, 2947
CfoI	GCGC	2 cut(s)	1546, 2882
Cfr13I	GGNCC	2 cut(s)	1857, 2969
CseI	GACGC	2 cut(s)	38, 2244
Csp6I	GTAC	3 cut(s)	1342, 2293, 2756
CviQI	GTAC	3 cut(s)	1342, 2293, 2756
DpnI	GATC	8 cut(s)	253, 544, 898, 1270, 1289, 1323, 1710, 2289
DpnII	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
DraI	TTTAAA	1 cut(s)	402
DriI	GACNNNNNGTC	1 cut(s)	2702
EaeI	YGGCCR	1 cut(s)	448
Eam1104I	CTCTTC	1 cut(s)	18
Eam1105I	GACNNNNNGTC	1 cut(s)	2702
EarI	CTCTTC	1 cut(s)	18
EciI	GGCGGA	1 cut(s)	264
Eco130I	CCWWGG	1 cut(s)	862
Eco147I	AGGCCT	1 cut(s)	2309
Eco24I	GRGCYC	1 cut(s)	2779
Eco32I	GATATC	3 cut(s)	967, 1131, 2895
Eco47I	GGWCC	1 cut(s)	2969
Eco57I	CTGAAG	1 cut(s)	491
Eco72I	CACGTG	1 cut(s)	2804
Eco81I	CCTNAGG	1 cut(s)	1849
EcoRII	CCWGG	7 cut(s)	113, 366, 475, 827, 969, 2323, 2518
EcoRV	GATATC	3 cut(s)	967, 1131, 2895
EcoT14I	CCWWGG	1 cut(s)	862
EcoT22I	ATGCAT	1 cut(s)	2065
EcoT38I	GRGCYC	1 cut(s)	2779
ErhI	CCWWGG	1 cut(s)	862
FalI	AAGNNNNNCTT	6 cut(s)	1506, 1538, 2484, 2516, 2543, 2575
FaqI	GGGAC	2 cut(s)	74, 398
FauI	CCCGC	1 cut(s)	136
FauNDI	CATATG	2 cut(s)	1635, 1717
FbaI	TGATCA	1 cut(s)	1287
FblI	GTMKAC	2 cut(s)	858, 3054
Fnu4HI	GCNGC	4 cut(s)	2109, 2534, 2832, 2862
FokI	GGATG	5 cut(s)	196, 383, 988, 2496, 2567
FriOI	GRGCYC	1 cut(s)	2779
Fsp4HI	GCNGC	4 cut(s)	2109, 2534, 2832, 2862
FspBI	CTAG	6 cut(s)	753, 1122, 1946, 2247, 2388, 2634
GlaI	GCGC	2 cut(s)	1545, 2881
GluI	GCNGC	4 cut(s)	2109, 2534, 2832, 2862
GsaI	CCCAGC	1 cut(s)	1896
GsuI	CTGGAG	2 cut(s)	315, 2909
HaeIII	GGCC	4 cut(s)	450, 1858, 2254, 2309
HapII	CCGG	2 cut(s)	1789, 2707
HgaI	GACGC	2 cut(s)	38, 2244
HhaI	GCGC	2 cut(s)	1546, 2882
Hin6I	GCGC	2 cut(s)	1544, 2880
HinP1I	GCGC	2 cut(s)	1544, 2880
HincII	GTYRAC	1 cut(s)	3055
HindII	GTYRAC	1 cut(s)	3055
HindIII	AAGCTT	5 cut(s)	1067, 1329, 1373, 2091, 2409
HpaII	CCGG	2 cut(s)	1789, 2707
HphI	GGTGA	7 cut(s)	1097, 1297, 1499, 1765, 2087, 2313, 2501
Hpy166II	GTNNAC	7 cut(s)	859, 1490, 1842, 2203, 2801, 3010, 3055
Hpy8I	GTNNAC	7 cut(s)	859, 1490, 1842, 2203, 2801, 3010, 3055
Hpy99I	CGWCG	2 cut(s)	54, 102
HpyCH4III	ACNGT	6 cut(s)	283, 617, 769, 1694, 2674, 2798
HpyCH4IV	ACGT	4 cut(s)	594, 2010, 2178, 2803
HpySE526I	ACGT	4 cut(s)	594, 2010, 2178, 2803
HspAI	GCGC	2 cut(s)	1544, 2880
Kpn2I	TCCGGA	2 cut(s)	1788, 2706
Ksp22I	TGATCA	1 cut(s)	1287
Kzo9I	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
LmnI	GCTCC	4 cut(s)	296, 2132, 2213, 2502
Lsp1109I	GCAGC	2 cut(s)	2095, 2843
LweI	GCATC	8 cut(s)	364, 1615, 2050, 2327, 2518, 2545, 2577, 3058
MaeI	CTAG	6 cut(s)	753, 1122, 1946, 2247, 2388, 2634
MaeII	ACGT	4 cut(s)	594, 2010, 2178, 2803
MaeIII	GTNAC	5 cut(s)	17, 595, 1163, 1649, 2174
MalI	GATC	8 cut(s)	253, 544, 898, 1270, 1289, 1323, 1710, 2289
MboI	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
MfeI	CAATTG	1 cut(s)	1658
MflI	RGATCY	2 cut(s)	251, 1708
MhlI	GDGCHC	4 cut(s)	2499, 2779, 2809, 3082
MlyI	GAGTC	4 cut(s)	592, 619, 992, 2987
MmeI	TCCRAC	2 cut(s)	1332, 2906
Mph1103I	ATGCAT	1 cut(s)	2065
MroI	TCCGGA	2 cut(s)	1788, 2706
MroXI	GAANNNNTTC	1 cut(s)	1238
MseI	TTAA	9 cut(s)	243, 335, 401, 612, 981, 1302, 2141, 2592, 2676
MslI	CAYNNNNRTG	6 cut(s)	438, 1108, 1638, 2453, 2469, 3005
MspA1I	CMGCKG	2 cut(s)	2359, 2984
MspI	CCGG	2 cut(s)	1789, 2707
MspR9I	CCNGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
MunI	CAATTG	1 cut(s)	1658
Mva1269I	GAATGC	1 cut(s)	1524
MvaI	CCWGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
MvnI	CGCG	1 cut(s)	49
NcoI	CCATGG	1 cut(s)	862
NdeI	CATATG	2 cut(s)	1635, 1717
NdeII	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
NlaIV	GGNNCC	2 cut(s)	273, 1595
NmuCI	GTSAC	4 cut(s)	17, 595, 1163, 1649
NsiI	ATGCAT	1 cut(s)	2065
NspI	RCATGY	2 cut(s)	1453, 3019
NspV	TTCGAA	1 cut(s)	993
OliI	CACNNNNGTG	1 cut(s)	1108
PagI	TCATGA	2 cut(s)	780, 2947
PceI	AGGCCT	1 cut(s)	2309
PciI	ACATGT	1 cut(s)	1449
PcsI	WCGNNNNNNNCGW	1 cut(s)	1346
PctI	GAATGC	1 cut(s)	1524
PdmI	GAANNNNTTC	1 cut(s)	1238
PflMI	CCANNNNNTGG	1 cut(s)	2519
PfoI	TCCNGGA	1 cut(s)	827
PkrI	GCNGC	4 cut(s)	2110, 2535, 2833, 2863
PleI	GAGTC	4 cut(s)	592, 619, 992, 2986
PmaCI	CACGTG	1 cut(s)	2804
PmlI	CACGTG	1 cut(s)	2804
PpsI	GAGTC	4 cut(s)	592, 619, 992, 2986
Ppu21I	YACGTR	1 cut(s)	2804
PscI	ACATGT	1 cut(s)	1449
Psp6I	CCWGG	7 cut(s)	113, 366, 475, 827, 969, 2323, 2518
PspCI	CACGTG	1 cut(s)	2804
PspFI	CCCAGC	1 cut(s)	1892
PspGI	CCWGG	7 cut(s)	113, 366, 475, 827, 969, 2323, 2518
PspN4I	GGNNCC	2 cut(s)	273, 1595
PspPI	GGNCC	2 cut(s)	1857, 2969
PsrI	GAACNNNNNNTAC	4 cut(s)	446, 472, 478, 504
PstI	CTGCAG	1 cut(s)	1909
PsuI	RGATCY	2 cut(s)	251, 1708
PvuII	CAGCTG	1 cut(s)	2359
RsaI	GTAC	3 cut(s)	1343, 2294, 2757
RsaNI	GTAC	3 cut(s)	1342, 2293, 2756
RseI	CAYNNNNRTG	6 cut(s)	438, 1108, 1638, 2453, 2469, 3005
SalI	GTCGAC	1 cut(s)	3053
SaqAI	TTAA	9 cut(s)	243, 335, 401, 612, 981, 1302, 2141, 2592, 2676
SatI	GCNGC	4 cut(s)	2109, 2534, 2832, 2862
Sau3AI	GATC	8 cut(s)	251, 542, 896, 1268, 1287, 1321, 1708, 2287
Sau96I	GGNCC	2 cut(s)	1857, 2969
SchI	GAGTC	4 cut(s)	592, 619, 992, 2987
ScrFI	CCNGG	7 cut(s)	115, 368, 477, 829, 971, 2325, 2520
SduI	GDGCHC	4 cut(s)	2499, 2779, 2809, 3082
SfaNI	GCATC	8 cut(s)	364, 1615, 2050, 2327, 2518, 2545, 2577, 3058
SfcI	CTRYAG	3 cut(s)	855, 1027, 1905
SfuI	TTCGAA	1 cut(s)	993
SinI	GGWCC	1 cut(s)	2969
SmiMI	CAYNNNNRTG	6 cut(s)	438, 1108, 1638, 2453, 2469, 3005
SmlI	CTYRAG	3 cut(s)	710, 2437, 2664
SmoI	CTYRAG	3 cut(s)	710, 2437, 2664
SseBI	AGGCCT	1 cut(s)	2309
SsiI	CCGC	5 cut(s)	143, 275, 2534, 2861, 2984
SspI	AATATT	2 cut(s)	1472, 3043
SspMI	CTAG	6 cut(s)	753, 1122, 1946, 2247, 2388, 2634
StuI	AGGCCT	1 cut(s)	2309
StyD4I	CCNGG	7 cut(s)	113, 366, 475, 827, 969, 2323, 2518
StyI	CCWWGG	1 cut(s)	862
TaaI	ACNGT	6 cut(s)	283, 617, 769, 1694, 2674, 2798
TaiI	ACGT	4 cut(s)	597, 2013, 2181, 2806
TaqI	TCGA	8 cut(s)	100, 152, 993, 1324, 1589, 2600, 2823, 3054
TaqII	GACCGA	1 cut(s)	535
TatI	WGTACW	1 cut(s)	2755
TauI	GCSGC	2 cut(s)	2536, 2864
Tru1I	TTAA	9 cut(s)	243, 335, 401, 612, 981, 1302, 2141, 2592, 2676
Tru9I	TTAA	9 cut(s)	243, 335, 401, 612, 981, 1302, 2141, 2592, 2676
TscAI	CASTG	7 cut(s)	622, 1654, 1837, 1849, 2210, 2230, 2544
TseFI	GTSAC	4 cut(s)	17, 595, 1163, 1649
TseI	GCWGC	2 cut(s)	2108, 2831
Tsp45I	GTSAC	4 cut(s)	17, 595, 1163, 1649
TspGWI	ACGGA	4 cut(s)	689, 1141, 1586, 2291
TspRI	CASTG	7 cut(s)	622, 1654, 1837, 1849, 2210, 2230, 2544
Van91I	CCANNNNNTGG	1 cut(s)	2519
VpaK11BI	GGWCC	1 cut(s)	2969
XapI	RAATTY	5 cut(s)	1784, 2149, 2257, 2329, 2783
XbaI	TCTAGA	3 cut(s)	1121, 1945, 2633
XceI	RCATGY	2 cut(s)	1453, 3019
XcmI	CCANNNNNNNNNTGG	1 cut(s)	667
XmiI	GTMKAC	2 cut(s)	858, 3054
XmnI	GAANNNNTTC	1 cut(s)	1238
XspI	CTAG	6 cut(s)	753, 1122, 1946, 2247, 2388, 2634
Zsp2I	ATGCAT	1 cut(s)	2065

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.