Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011243

Physical Location & Seq

Genomic Coordinates Forward (+)

32506807 .. 32516421

Gene Structure 9615 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1221900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3261 bp

ATGGCTTTGGGGTTCTCCTCCATCCAGACCAGCCATTTAAGACCATCTTGCCCTTCTTATTTCTCCTTATTCCGCTCCTGTCCCCGGTCGTCCAGGTCCCTGTGGACTAGTCCCAGACCACAGGCTTATGCGGCCCAGGCTGATGTTGGGATTTCAGGTAATCCGACAATCCCTTTCAACCCAGTTCACTATAATCCCGAAAGAAAATCCAACGATAATAGAAAAGCCTGGAAACACGAGGCTTGGGCATTGAGAAAGGAAAAGGTGAAGGAAAATCCTTATGGAAGTAACATATCCTGTAGAGTAAATATTGAAACATATACGCCAGAAAGGAATGAGGGTTCCCTACATTTGGATGATAAGTTGGATGTAAAGTCTGAAGCCTCATTTTACAAAACATCGGCAATTGATGATCAAATCAGTGAAAGTTTTGCAAACATACATGAGGCTGAGGCTTCTCCTGAGGTACAATCGGGTAAAGCACATGGTGAAGGAAATAATGTTGACAAGGTGAGTGGTTATGGAGGGATTGACAAGATTAAAGTGGACAAGGTGAATGGTTACAGACGGGGCAGTCAGAATGTGTCGGCTTATGGAGCGATTGACAAGATTAAGGTGAACAAGGTGAATGGTCATGGACGGGACCATCAGAATGTGTCCGCTTATGGAGGAGTTGATAAGATAAAAGTGGACAAGGTGAATGGTTATGGACAGGACCGTCAGAATGTGTCAGCTTCTGGAGGGATTGACAATATTAAAGTGAAGAAGGTGAATGGTCATGCAGATGCTGCTCATGTGATTCAAAGGGACAAGTCAGCTAAAAAACCTTCTGAAACTAAAGCTGCTACAGCAATAAATGAGGGAGATCTTCTTGAGAAGCTTAGTACCATATATGACAAGGTTCTTGTTGTCAACAGTGTCCCTGATGCGAAAAAAGTTGTTAGGATGCTTACAGATCAATACAGGCATCTTGTCCATGCCTGTGACACAGAGGTGGCGAATATAGAGGTGAAGCTAGAAACACCTGTTGATCATGGAGAAATAATATGTTTTAGCATCTATTCTGGACCAGATGTGGATTTTGGAAATGGGAAGTCTTGTATTTGGGTGGATGTTCTTGATGGTGGTGGCAAGGAACTTTTAATGGAGTTTGCTCCTTTTTTCGAAGACCCCTCCATCAAAAAGGTTTGGCACAATTATAGCTTTGATAACCATGTAATAGAGAACTATGGGATTAAGCTTTCTGGTTTCCATGCCGACACGATGCACATGGCTCGCTTGTGGGATTCATCAAGGCGGTTAAAAGGTGGTTATTCTCTTGAAGCACTTTCACATGACCCAAAGGTCATGTCTGGGGCCCAACTGAGTGATGAGAAGGATTTGATTGGTAAAATATCTATGAAAACAATATTTGGCAGGAAGAAAGTGAAGAAAGATGGAAGTGACGGGAAAGTGACCATTATTGACCCTGTTGAAGTGCTACAAAGAGAAAAGAGAAAACCATGGATTTGTTATTCTGCCTTAGATTCAATAAGCACACTGAAGCTTTATGAGAGTATGAAAAACCAACTAAGCAAAAAGGAGTGGAAAATTGATGGAAAACCAGAACCTGGAACCATGTTTAATTTCTATGAAAAATATTGGAGACCATTTGGTGAGCTTTTAGTTAAGATGGAAACTGAGGGAATGTTGGTTAATCGGGATTATTTAGCTGAGATTGAGAAGTTGGCCAAAGCAGAACAAGAGATTGCTGCAAACAGATTTCGTAGGTGGGCGTCTAGGTACTGCCCTGATGCCAACTACATGAATGTGGGAAGTGATGTGCAACTACGCCAGCTCCTCTTTGGTGGGATCGCAAATAGAAAGGACTCCAACCAGTTTCTTCCAACGGAGAAGACTTTCAGAGTTCCAAACATTGATGGAGTGATTGAAGAAGGGAAGAATACAGCCTCAAAATTTCGCAATATTACTCTGCATAATATTGTTTCCAATCTTCCAGCTGAGATTTACACAGCAAGTGGTTGGCCCTCAGTTGGTGGAGATGCTTTGAAAATTTTATCTGGGAAGGTCTCTTCAGAATTTCATTTTGTGGATGACGACCAGGAAGGTGTTGGAGATGCTTGTGAGACAGTGGATGCTGAATATTTGGGAACACAAGAGGATAGGTCTGAGGTTGACACATCTGCTTATGGAACAGCTTTTAAAGCATTTGAGAAAGAGGAGAAGGGGAAAGGGAAGGAAGCTTGCCACGCCATTGCTGCTTTATGCCAAGTTTGCTCTATTGATTCCTTGATATCCAACTTCATCCTTCCTTTGCAGAGCAGTAATATATCAGGCAAGAATAAGCGAATTCATTGTTCTCTAAATATCAACACAGAAACGGGGCGTCTGTCCGCGAGGAGGCCTAATTTACAGAACCAACCTGCTTTGGAGAAAGACCGGTATAAGATCCGCCAGGCTTTTGTAGCTGCACCCGGGAATTCTCTTATTGTTGCTGATTATGGACAGCTCGAACTCAGGATTCTTGCTCATCTGTCCAACTGTACAAGTATGTTGGATGCCTTCAAAGCTGGTGGAGATTTTCATTCAAGGACTGCAATGAACATGTACCAGCATATTCGTGATGCCGTTGAGAGTGAAGAAGTGCTACTTGAGTGGGATCCTCAACCTGGTGAAGAGAAACCCCCAGTTCCATTATTGAAGGATAACTTTGCTTCTGAAAGAAGGAAAGCTAAAATGCTTAACTTTTCCATTGCATATGGGAAAACTCCAGTTGGGCTTTCCCGGGATTGGAAGGTATCGGTTCAGGAAGCAGAAAACACAGTTAAACTGTGGTACAAAGAAAGACAAGAAGTGCTTATTTGGCAAGAAGAACGTAAAAAAGAAGCTATAGAAAATAGATGTGTTCACACATTACTTGGACGGGCTCGCCAGTTCCCAGCAGTATCTCGTGATAAACGTGCTCTAAGAGGTCATATTGAAAGGGCTGCTATCAATACACCAGTGCAGGGCAGTGCTGCTGATGTTGCCATGTGTGCTATGTTAGAAATATCCAAGAATGCACGTTTGAATGAACTTGGGTGGAGGCTGCTTTTACAGGTTCACGATGAAGTGATCCTGGAAGGGCCATCAGAGTCTGCTGAGGAAGCAAAGAAATTAGTTGTTGAGTGCATGTCGTTTCCCTTCAACGGAAAGAATAATCTTAAAGTCGACCTTGCCGTTGATGCCAAGTGTGCACAAAACTGGTATGCCGGCAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0003674 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006264 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009657 GO:0009987 GO:0016043 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0032042 GO:0033258 GO:0033259 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03440 map00230 map00240 map01100 map03030 map03410 map03420 map03440

Pfam Domains

Protein Families

DNA_pol_A DNA_pol_A_exo1

Protein Analysis

1087

Amino Acids

121.36

Weight (kDa)

7.93

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_A_exo1	PF01612	361 - 524	1.6e-10	3'-5' exonuclease
DNA_pol_A	PF00476	750 - 880	1.3e-30	DNA polymerase family A
DNA_pol_A	PF00476	905 - 1083	1.7e-45	DNA polymerase family A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50840 AT1G50840 AT3G20540 AT3G20540
fragaria_vesca	FvH4_3g22230
malus_domestica	MD11G1221900.v1.1
prunus_persica	Prupe.4G209500_v2.0.a1 Prupe.4G209500_v2.0.a1 Prupe.4G209500_v2.0.a1
pyrus_communis	pycom11g19570
rosa_chinensis	RchiOBHm_Chr5g0038301
rosa_laevigata	RLG00000033845
rosa_multiflora	Rmu_co8325491.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042720
rosa_rugosa	Rorug05G0171900
rosa_samantha	Rh5AG259200 Rh5BG263000 Rh5CG295400 Rh5DG272000
rosa_wichuraiana	Rw5G024260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	573, 717, 1343
Acc36I	ACCTGC	1 cut(s)	2431
AccB7I	CCANNNNNTGG	1 cut(s)	1610
AccBSI	CCGCTC	1 cut(s)	75
AccI	GTMKAC	1 cut(s)	3210
AccII	CGCG	1 cut(s)	2396
AciI	CCGC	6 cut(s)	73, 131, 660, 1297, 2394, 2452
AclWI	GGATC	5 cut(s)	1859, 2443, 2654, 2667, 3109
AcoI	YGGCCR	1 cut(s)	1728
AcsI	RAATTY	5 cut(s)	1955, 2052, 2078, 2349, 2479
AcuI	CTGAAG	3 cut(s)	399, 1562, 2058
AcyI	GRCGYC	2 cut(s)	1775, 2386
AdeI	CACNNNGTG	1 cut(s)	488
AfaI	GTAC	6 cut(s)	468, 886, 1784, 2545, 2609, 2837
AfiI	CCNNNNNNNGG	9 cut(s)	84, 352, 1610, 2033, 2400, 2669, 2790, 3008, 3188
AflIII	ACRYGT	1 cut(s)	2604
AgeI	ACCGGT	1 cut(s)	2439
AhlI	ACTAGT	1 cut(s)	107
AjnI	CCWGG	8 cut(s)	92, 135, 227, 1609, 2100, 2454, 2668, 3117
AleI	CACNNNNGTG	1 cut(s)	992
AloI	GAACNNNNNNTCC	2 cut(s)	325, 357
Alw21I	GWGCWC	2 cut(s)	2965, 3238
Alw26I	GTCTC	3 cut(s)	1639, 2074, 2120
Alw44I	GTGCAC	1 cut(s)	3234
AlwI	GGATC	5 cut(s)	1859, 2443, 2654, 2667, 3109
AlwNI	CAGNNNCTG	4 cut(s)	737, 788, 1610, 3137
Ama87I	CYCGRG	2 cut(s)	2474, 2784
AoxI	GGCC	6 cut(s)	132, 1356, 1728, 2024, 2402, 3125
ApaI	GGGCCC	1 cut(s)	1360
ApaLI	GTGCAC	1 cut(s)	3234
ApeKI	GCWGC	8 cut(s)	788, 842, 1751, 2258, 2468, 2987, 3017, 3088
ApoI	RAATTY	5 cut(s)	1955, 2052, 2078, 2349, 2479
ArsI	GACNNNNNNTTYG	2 cut(s)	1334, 1366
AsiGI	ACCGGT	1 cut(s)	2439
Asp700I	GAANNNNTTC	1 cut(s)	1898
AspS9I	GGNCC	9 cut(s)	96, 133, 643, 715, 1067, 1356, 1357, 2025, 3125
AsuC2I	CCSGG	5 cut(s)	85, 2475, 2476, 2785, 2786
AsuII	TTCGAA	1 cut(s)	1164
AvaI	CYCGRG	2 cut(s)	2474, 2784
AvaII	GGWCC	4 cut(s)	96, 643, 715, 1067
AxyI	CCTNAGG	1 cut(s)	462
BaeGI	GKGCMC	2 cut(s)	1360, 3238
BalI	TGGCCA	1 cut(s)	1730
BamHI	GGATCC	1 cut(s)	2659
BanII	GRGCYC	2 cut(s)	1360, 2929
BarI	GAAGNNNNNNTAC	2 cut(s)	2631, 2663
BauI	CACGAG	2 cut(s)	236, 2949
BbsI	GAAGAC	2 cut(s)	1173, 1901
Bbv12I	GWGCWC	2 cut(s)	2965, 3238
BbvCI	CCTCAGC	2 cut(s)	450, 3141
BbvI	GCAGC	8 cut(s)	775, 829, 1738, 2245, 2455, 2974, 3004, 3075
BceAI	ACGGC	2 cut(s)	2612, 3203
BcgI	CGANNNNNNTGC	2 cut(s)	1256, 1290
BciT130I	CCWGG	8 cut(s)	94, 137, 229, 1611, 2102, 2456, 2670, 3119
BclI	TGATCA	2 cut(s)	412, 1030
BcnI	CCSGG	5 cut(s)	85, 2475, 2476, 2785, 2786
BcoDI	GTCTC	3 cut(s)	1639, 2074, 2120
BcuI	ACTAGT	1 cut(s)	107
BfaI	CTAG	3 cut(s)	108, 1016, 1779
BfmI	CTRYAG	3 cut(s)	298, 846, 2889
BfuAI	ACCTGC	1 cut(s)	2431
BglII	AGATCT	1 cut(s)	865
BisI	GCNGC	9 cut(s)	132, 789, 843, 1752, 2259, 2469, 2988, 3018, 3089
BlsI	GCNGC	9 cut(s)	133, 790, 844, 1753, 2260, 2470, 2989, 3019, 3090
Bme18I	GGWCC	4 cut(s)	96, 643, 715, 1067
BmeT110I	CYCGRG	2 cut(s)	2474, 2784
BmgT120I	GGNCC	9 cut(s)	96, 133, 643, 715, 1067, 1356, 1357, 2025, 3125
BmiI	GGNNCC	7 cut(s)	98, 343, 644, 1357, 1358, 1615, 2661
BmrI	ACTGGG	2 cut(s)	176, 2681
BmuI	ACTGGG	2 cut(s)	176, 2681
BpiI	GAAGAC	2 cut(s)	1173, 1901
BpmI	CTGGAG	2 cut(s)	759, 2754
Bpu10I	CCTNAGC	2 cut(s)	450, 3141
Bpu14I	TTCGAA	1 cut(s)	1164
BpuEI	CTTGAG	2 cut(s)	893, 2672
BpuMI	CCSGG	5 cut(s)	85, 2475, 2476, 2785, 2786
BsaHI	GRCGYC	2 cut(s)	1775, 2386
BsaI	GGTCTC	2 cut(s)	1639, 2074
BsaJI	CCNNGG	5 cut(s)	83, 135, 1502, 2474, 2784
BsaWI	WCCGGW	1 cut(s)	2439
BsaXI	ACNNNNNCTCC	2 cut(s)	1821, 1851
Bsc4I	CCNNNNNNNGG	9 cut(s)	84, 352, 1610, 2033, 2400, 2669, 2790, 3008, 3188
Bse118I	RCCGGY	2 cut(s)	2439, 3251
Bse1I	ACTGG	7 cut(s)	182, 1876, 2687, 2771, 2932, 3002, 3248
Bse21I	CCTNAGG	1 cut(s)	462
Bse3DI	GCAATG	3 cut(s)	2253, 2604, 2751
BseBI	CCWGG	8 cut(s)	94, 137, 229, 1611, 2102, 2456, 2670, 3119
BseDI	CCNNGG	5 cut(s)	83, 135, 1502, 2474, 2784
BseGI	GGATG	9 cut(s)	21, 361, 373, 951, 1117, 2098, 2140, 2304, 2563
BseLI	CCNNNNNNNGG	9 cut(s)	84, 352, 1610, 2033, 2400, 2669, 2790, 3008, 3188
BseMI	GCAATG	3 cut(s)	2253, 2604, 2751
BseNI	ACTGG	7 cut(s)	182, 1876, 2687, 2771, 2932, 3002, 3248
BseRI	GAGGAG	5 cut(s)	7, 684, 1829, 2234, 2413
BseSI	GKGCMC	2 cut(s)	1360, 3238
BseXI	GCAGC	8 cut(s)	775, 829, 1738, 2245, 2455, 2974, 3004, 3075
BseYI	CCCAGC	1 cut(s)	2938
BsgI	GTGCAG	2 cut(s)	2454, 3026
Bsh1236I	CGCG	1 cut(s)	2396
Bsh1285I	CGRYCG	1 cut(s)	89
BshFI	GGCC	6 cut(s)	134, 1358, 1730, 2026, 2404, 3127
BshTI	ACCGGT	1 cut(s)	2439
BsiEI	CGRYCG	1 cut(s)	89
BsiHKAI	GWGCWC	2 cut(s)	2965, 3238
BsiHKCI	CYCGRG	2 cut(s)	2474, 2784
BsiSI	CCGG	5 cut(s)	85, 2440, 2475, 2785, 3252
BslFI	GGGAC	6 cut(s)	66, 82, 96, 656, 821, 905
BslI	CCNNNNNNNGG	9 cut(s)	84, 352, 1610, 2033, 2400, 2669, 2790, 3008, 3188
BsmAI	GTCTC	3 cut(s)	1639, 2074, 2120
BsmFI	GGGAC	6 cut(s)	66, 82, 96, 656, 821, 905
BsmI	GAATGC	1 cut(s)	3064
BsnI	GGCC	6 cut(s)	134, 1358, 1730, 2026, 2404, 3127
Bso31I	GGTCTC	2 cut(s)	1639, 2074
BsoBI	CYCGRG	2 cut(s)	2474, 2784
Bsp119I	TTCGAA	1 cut(s)	1164
Bsp120I	GGGCCC	1 cut(s)	1356
Bsp1286I	GDGCHC	4 cut(s)	1360, 2929, 2965, 3238
Bsp1407I	TGTACA	1 cut(s)	2543
Bsp143I	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
Bsp19I	CCATGG	1 cut(s)	1502
BspACI	CCGC	6 cut(s)	73, 131, 660, 1297, 2394, 2452
BspANI	GGCC	6 cut(s)	134, 1358, 1730, 2026, 2404, 3127
BspFNI	CGCG	1 cut(s)	2396
BspLI	GGNNCC	7 cut(s)	98, 343, 644, 1357, 1358, 1615, 2661
BspMI	ACCTGC	1 cut(s)	2431
BspPI	GGATC	5 cut(s)	1859, 2443, 2654, 2667, 3109
BspT104I	TTCGAA	1 cut(s)	1164
BspTNI	GGTCTC	2 cut(s)	1639, 2074
BsrBI	CCGCTC	1 cut(s)	75
BsrDI	GCAATG	3 cut(s)	2253, 2604, 2751
BsrFI	RCCGGY	2 cut(s)	2439, 3251
BsrGI	TGTACA	1 cut(s)	2543
BsrI	ACTGG	7 cut(s)	182, 1876, 2687, 2771, 2932, 3002, 3248
BssAI	RCCGGY	2 cut(s)	2439, 3251
BssECI	CCNNGG	5 cut(s)	83, 135, 1502, 2474, 2784
BssMI	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
BssNI	GRCGYC	2 cut(s)	1775, 2386
BssSI	CACGAG	2 cut(s)	236, 2949
BssT1I	CCWWGG	1 cut(s)	1502
Bst2BI	CACGAG	2 cut(s)	236, 2949
Bst2UI	CCWGG	8 cut(s)	94, 137, 229, 1611, 2102, 2456, 2670, 3119
Bst4CI	ACNGT	6 cut(s)	719, 917, 2131, 2543, 2824, 2832
Bst6I	CTCTTC	2 cut(s)	2077, 2670
BstACI	GRCGYC	2 cut(s)	1775, 2386
BstAPI	GCANNNNNTGC	1 cut(s)	788
BstAUI	TGTACA	1 cut(s)	2543
BstBI	TTCGAA	1 cut(s)	1164
BstC8I	GCNNGC	5 cut(s)	1276, 1835, 2245, 2929, 3253
BstDSI	CCRYGG	1 cut(s)	1502
BstF5I	GGATG	9 cut(s)	21, 361, 373, 951, 1117, 2098, 2140, 2304, 2563
BstFNI	CGCG	1 cut(s)	2396
BstKTI	GATC	8 cut(s)	415, 868, 958, 1033, 1854, 2451, 2662, 3117
BstMAI	GTCTC	3 cut(s)	1639, 2074, 2120
BstMBI	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
BstMCI	CGRYCG	1 cut(s)	89
BstNI	CCWGG	8 cut(s)	94, 137, 229, 1611, 2102, 2456, 2670, 3119
BstNSI	RCATGY	2 cut(s)	2608, 3175
BstSFI	CTRYAG	3 cut(s)	298, 846, 2889
BstSLI	GKGCMC	2 cut(s)	1360, 3238
BstUI	CGCG	1 cut(s)	2396
BstV1I	GCAGC	8 cut(s)	775, 829, 1738, 2245, 2455, 2974, 3004, 3075
BstV2I	GAAGAC	2 cut(s)	1173, 1901
BstX2I	RGATCY	3 cut(s)	865, 2448, 2659
BstYI	RGATCY	3 cut(s)	865, 2448, 2659
Bsu36I	CCTNAGG	1 cut(s)	462
BsuRI	GGCC	6 cut(s)	134, 1358, 1730, 2026, 2404, 3127
BtgI	CCRYGG	1 cut(s)	1502
BtsCI	GGATG	9 cut(s)	21, 361, 373, 951, 1117, 2098, 2140, 2304, 2563
BtsI	GCAGTG	1 cut(s)	3019
BtsIMutI	CAGTG	6 cut(s)	427, 922, 1538, 2136, 3009, 3019
BveI	ACCTGC	1 cut(s)	2431
Cac8I	GCNNGC	5 cut(s)	1276, 1835, 2245, 2929, 3253
CaiI	CAGNNNCTG	4 cut(s)	737, 788, 1610, 3137
Cfr10I	RCCGGY	2 cut(s)	2439, 3251
Cfr13I	GGNCC	9 cut(s)	96, 133, 643, 715, 1067, 1356, 1357, 2025, 3125
Cfr9I	CCCGGG	2 cut(s)	2474, 2784
CseI	GACGC	2 cut(s)	1764, 2375
CsiI	ACCWGGT	1 cut(s)	2668
Csp6I	GTAC	6 cut(s)	467, 885, 1783, 2544, 2608, 2836
CspAI	ACCGGT	1 cut(s)	2439
CspCI	CAANNNNNGTGG	4 cut(s)	496, 531, 2554, 2589
CviQI	GTAC	6 cut(s)	467, 885, 1783, 2544, 2608, 2836
DpnI	GATC	8 cut(s)	414, 867, 957, 1032, 1853, 2450, 2661, 3116
DpnII	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
DraI	TTTAAA	1 cut(s)	2203
DraIII	CACNNNGTG	1 cut(s)	488
DrdI	GACNNNNNNGTC	3 cut(s)	573, 717, 1343
DseDI	GACNNNNNNGTC	3 cut(s)	573, 717, 1343
EaeI	YGGCCR	1 cut(s)	1728
Eam1104I	CTCTTC	2 cut(s)	2077, 2670
EarI	CTCTTC	2 cut(s)	2077, 2670
EciI	GGCGGA	1 cut(s)	2441
Eco130I	CCWWGG	1 cut(s)	1502
Eco147I	AGGCCT	1 cut(s)	2404
Eco24I	GRGCYC	2 cut(s)	1360, 2929
Eco31I	GGTCTC	2 cut(s)	1639, 2074
Eco32I	GATATC	1 cut(s)	2295
Eco47I	GGWCC	4 cut(s)	96, 643, 715, 1067
Eco57I	CTGAAG	3 cut(s)	399, 1562, 2058
Eco81I	CCTNAGG	1 cut(s)	462
Eco88I	CYCGRG	2 cut(s)	2474, 2784
EcoO109I	RGGNCCY	2 cut(s)	96, 1356
EcoRI	GAATTC	2 cut(s)	2349, 2479
EcoRII	CCWGG	8 cut(s)	92, 135, 227, 1609, 2100, 2454, 2668, 3117
EcoRV	GATATC	1 cut(s)	2295
EcoT14I	CCWWGG	1 cut(s)	1502
EcoT38I	GRGCYC	2 cut(s)	1360, 2929
ErhI	CCWWGG	1 cut(s)	1502
FaqI	GGGAC	6 cut(s)	66, 82, 96, 656, 821, 905
FauNDI	CATATG	1 cut(s)	2758
FbaI	TGATCA	2 cut(s)	412, 1030
FblI	GTMKAC	1 cut(s)	3210
Fnu4HI	GCNGC	9 cut(s)	132, 789, 843, 1752, 2259, 2469, 2988, 3018, 3089
FokI	GGATG	9 cut(s)	8, 368, 380, 958, 1124, 2105, 2147, 2291, 2570
FriOI	GRGCYC	2 cut(s)	1360, 2929
Fsp4HI	GCNGC	9 cut(s)	132, 789, 843, 1752, 2259, 2469, 2988, 3018, 3089
FspBI	CTAG	3 cut(s)	108, 1016, 1779
GluI	GCNGC	9 cut(s)	132, 789, 843, 1752, 2259, 2469, 2988, 3018, 3089
GsaI	CCCAGC	1 cut(s)	2942
GsuI	CTGGAG	2 cut(s)	759, 2754
HaeIII	GGCC	6 cut(s)	134, 1358, 1730, 2026, 2404, 3127
HapII	CCGG	5 cut(s)	85, 2440, 2475, 2785, 3252
HgaI	GACGC	2 cut(s)	1764, 2375
Hin1I	GRCGYC	2 cut(s)	1775, 2386
HincII	GTYRAC	4 cut(s)	505, 913, 2176, 3211
HindII	GTYRAC	4 cut(s)	505, 913, 2176, 3211
HindIII	AAGCTT	4 cut(s)	878, 1238, 1544, 2241
HinfI	GANTC	7 cut(s)	799, 1286, 1526, 1868, 2285, 2521, 3134
HpaII	CCGG	5 cut(s)	85, 2440, 2475, 2785, 3252
HpyCH4III	ACNGT	6 cut(s)	719, 917, 2131, 2543, 2824, 2832
HpyCH4IV	ACGT	3 cut(s)	2875, 2959, 3064
HpySE526I	ACGT	3 cut(s)	2875, 2959, 3064
Hsp92I	GRCGYC	2 cut(s)	1775, 2386
KroI	GCCGGC	1 cut(s)	3251
KroNI	GCCGGC	1 cut(s)	3253
Ksp22I	TGATCA	2 cut(s)	412, 1030
Kzo9I	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
LmnI	GCTCC	4 cut(s)	80, 596, 1159, 1842
Lsp1109I	GCAGC	8 cut(s)	775, 829, 1738, 2245, 2455, 2974, 3004, 3075
MabI	ACCWGGT	1 cut(s)	2668
MaeI	CTAG	3 cut(s)	108, 1016, 1779
MaeII	ACGT	3 cut(s)	2875, 2959, 3064
MaeIII	GTNAC	5 cut(s)	287, 560, 983, 1442, 1453
MalI	GATC	8 cut(s)	414, 867, 957, 1032, 1853, 2450, 2661, 3116
MbiI	CCGCTC	1 cut(s)	75
MboI	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
MfeI	CAATTG	1 cut(s)	405
MflI	RGATCY	3 cut(s)	865, 2448, 2659
MhlI	GDGCHC	4 cut(s)	1360, 2929, 2965, 3238
MlsI	TGGCCA	1 cut(s)	1730
MluNI	TGGCCA	1 cut(s)	1730
MlyI	GAGTC	2 cut(s)	1862, 3143
MmeI	TCCRAC	9 cut(s)	188, 234, 345, 1896, 1910, 2092, 2322, 2535, 2562
Mox20I	TGGCCA	1 cut(s)	1730
MroNI	GCCGGC	1 cut(s)	3251
MroXI	GAANNNNTTC	1 cut(s)	1898
MscI	TGGCCA	1 cut(s)	1730
MslI	CAYNNNNRTG	7 cut(s)	354, 651, 783, 981, 992, 1808, 2619
Msp20I	TGGCCA	1 cut(s)	1730
MspA1I	CMGCKG	1 cut(s)	2000
MspI	CCGG	5 cut(s)	85, 2440, 2475, 2785, 3252
MunI	CAATTG	1 cut(s)	405
Mva1269I	GAATGC	1 cut(s)	3064
MvaI	CCWGG	8 cut(s)	94, 137, 229, 1611, 2102, 2456, 2670, 3119
MvnI	CGCG	1 cut(s)	2396
NaeI	GCCGGC	1 cut(s)	3253
NciI	CCSGG	5 cut(s)	85, 2475, 2476, 2785, 2786
NcoI	CCATGG	1 cut(s)	1502
NdeI	CATATG	1 cut(s)	2758
NdeII	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
NgoMIV	GCCGGC	1 cut(s)	3251
NlaIV	GGNNCC	7 cut(s)	98, 343, 644, 1357, 1358, 1615, 2661
NmuCI	GTSAC	3 cut(s)	983, 1442, 1453
NspI	RCATGY	2 cut(s)	2608, 3175
NspV	TTCGAA	1 cut(s)	1164
OliI	CACNNNNGTG	1 cut(s)	992
PceI	AGGCCT	1 cut(s)	2404
PciI	ACATGT	1 cut(s)	2604
PcsI	WCGNNNNNNNCGW	2 cut(s)	2956, 3216
PctI	GAATGC	1 cut(s)	3064
PdiI	GCCGGC	1 cut(s)	3253
PdmI	GAANNNNTTC	1 cut(s)	1898
PfeI	GAWTC	5 cut(s)	799, 1286, 1526, 2285, 2521
PflMI	CCANNNNNTGG	1 cut(s)	1610
PfoI	TCCNGGA	1 cut(s)	3117
PinAI	ACCGGT	1 cut(s)	2439
PkrI	GCNGC	9 cut(s)	133, 790, 844, 1753, 2260, 2470, 2989, 3019, 3090
PleI	GAGTC	2 cut(s)	1862, 3142
PpsI	GAGTC	2 cut(s)	1862, 3142
PpuMI	RGGWCCY	1 cut(s)	96
PscI	ACATGT	1 cut(s)	2604
Psp5II	RGGWCCY	1 cut(s)	96
Psp6I	CCWGG	8 cut(s)	92, 135, 227, 1609, 2100, 2454, 2668, 3117
PspFI	CCCAGC	1 cut(s)	2938
PspGI	CCWGG	8 cut(s)	92, 135, 227, 1609, 2100, 2454, 2668, 3117
PspN4I	GGNNCC	7 cut(s)	98, 343, 644, 1357, 1358, 1615, 2661
PspOMI	GGGCCC	1 cut(s)	1356
PspPI	GGNCC	9 cut(s)	96, 133, 643, 715, 1067, 1356, 1357, 2025, 3125
PspPPI	RGGWCCY	1 cut(s)	96
PstNI	CAGNNNCTG	4 cut(s)	737, 788, 1610, 3137
PsuI	RGATCY	3 cut(s)	865, 2448, 2659
PvuII	CAGCTG	1 cut(s)	2000
RsaI	GTAC	6 cut(s)	468, 886, 1784, 2545, 2609, 2837
RsaNI	GTAC	6 cut(s)	467, 885, 1783, 2544, 2608, 2836
RseI	CAYNNNNRTG	7 cut(s)	354, 651, 783, 981, 992, 1808, 2619
SalI	GTCGAC	1 cut(s)	3209
SatI	GCNGC	9 cut(s)	132, 789, 843, 1752, 2259, 2469, 2988, 3018, 3089
Sau3AI	GATC	8 cut(s)	412, 865, 955, 1030, 1851, 2448, 2659, 3114
Sau96I	GGNCC	9 cut(s)	96, 133, 643, 715, 1067, 1356, 1357, 2025, 3125
SchI	GAGTC	2 cut(s)	1862, 3143
SduI	GDGCHC	4 cut(s)	1360, 2929, 2965, 3238
SexAI	ACCWGGT	1 cut(s)	2668
SfcI	CTRYAG	3 cut(s)	298, 846, 2889
SfuI	TTCGAA	1 cut(s)	1164
SinI	GGWCC	4 cut(s)	96, 643, 715, 1067
SmaI	CCCGGG	2 cut(s)	2476, 2786
SmiMI	CAYNNNNRTG	7 cut(s)	354, 651, 783, 981, 992, 1808, 2619
SmlI	CTYRAG	2 cut(s)	872, 2651
SmoI	CTYRAG	2 cut(s)	872, 2651
SpeI	ACTAGT	1 cut(s)	107
SseBI	AGGCCT	1 cut(s)	2404
SsiI	CCGC	6 cut(s)	73, 131, 660, 1297, 2394, 2452
SspI	AATATT	7 cut(s)	310, 754, 1410, 1640, 1966, 1981, 2144
SspMI	CTAG	3 cut(s)	108, 1016, 1779
StuI	AGGCCT	1 cut(s)	2404
StyI	CCWWGG	1 cut(s)	1502
TaaI	ACNGT	6 cut(s)	719, 917, 2131, 2543, 2824, 2832
TaiI	ACGT	3 cut(s)	2878, 2962, 3067
TaqI	TCGA	3 cut(s)	1164, 2511, 3210
TatI	WGTACW	1 cut(s)	2543
TauI	GCSGC	1 cut(s)	134
TfiI	GAWTC	5 cut(s)	799, 1286, 1526, 2285, 2521
TscAI	CASTG	6 cut(s)	427, 922, 1545, 2136, 3009, 3019
TseFI	GTSAC	3 cut(s)	983, 1442, 1453
TseI	GCWGC	8 cut(s)	788, 842, 1751, 2258, 2468, 2987, 3017, 3088
Tsp45I	GTSAC	3 cut(s)	983, 1442, 1453
TspGWI	ACGGA	2 cut(s)	1904, 3204
TspMI	CCCGGG	2 cut(s)	2474, 2784
TspRI	CASTG	6 cut(s)	427, 922, 1545, 2136, 3009, 3019
Van91I	CCANNNNNTGG	1 cut(s)	1610
VneI	GTGCAC	1 cut(s)	3234
VpaK11BI	GGWCC	4 cut(s)	96, 643, 715, 1067
XapI	RAATTY	5 cut(s)	1955, 2052, 2078, 2349, 2479
XceI	RCATGY	2 cut(s)	2608, 3175
XcmI	CCANNNNNNNNNTGG	3 cut(s)	143, 1841, 2108
XmaI	CCCGGG	2 cut(s)	2474, 2784
XmiI	GTMKAC	1 cut(s)	3210
XmnI	GAANNNNTTC	1 cut(s)	1898
XspI	CTAG	3 cut(s)	108, 1016, 1779

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.