Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0011243

Physical Location & Seq

Genomic Coordinates Reverse (-)

33090323 .. 33099412

Gene Structure 9090 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG272000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2952 bp

ATGGCTATGGGGTTCTCCTCCTTCCAGACCAGCCATTTCAGGCCTTGCCCTTCTTATTTCTCTTTATTACGGGCTTGTCCTCTGTCTTCTTCGTCTTCTAGGGCCCTATGGACTCCTTCAGTTTCAAGGCGAGTTTTGGATTGCAAGCCAGAGGTCTATGTTGGGCGCCTAAGGGGTGTCCAGGCTGCCAAATCATATAATACTCTGACCTCTGAAGGAAAATCCAACGACTCTAGATATGCCTGGAGAGCTGCGGTTACTCGCCTGAGGAAAGAAAAACAGGAAGTGGAAAGTTCTTACAGAAGTAAGACATACTCTGAAGGAAATATAAAAACGATTACCCCTGAAAAGGTTGATAACAGTAGCTATGAGATGGGAGAGGTGACTTTATATGAGACACATGGTGAAGGAAGTAATGTGGACAGAGTAAACGGTTATGCACACGATAATCCAGATGTAGTAGCTTCTGTAGGAATTGACAATCCTAGTGTGGAAAAGGTGAATGGTTATGCACATGATGGTCCAAGACTGAGTGTGGCTACGCCACCCAGATCAGGGAAGGAAGTTAAGTCCAAGGGGCCATTATTGGGGAAGACCTCAAAGGTCAGGAAAGGGATTGATTCTGGACCTAGTAAAGATGAAGCCGGTAATGTGATGAACAAGGACAAGTCCGATGTAAAATCTTCTGAATCTAAAGCTGCTACACCAGTACATAGTAAGGAAGATCTTCGCAAGACACTTAATAACATTTACGACAAAGTTCTCATTGTCAATAGTGTCTCTATTGCAAAGAAAGTCGTTAGGATGCTTAGAGGTGAATATAGGCATCTTGTCCATGCCTGTGACACAGAGGTGGCCGAGATAGATGTTAAGAAAGAAACACCTGTAGATCATGGACAGATAATATGCTTCAGTATATATTCTGGACCAGATGTGGATTTTGGAAATGGAAAGTCATGCATCTGGGTGGATGTTCTTGATGGTGGCGGCAAGGAACTTCTACTTGAATTTGCTCCATTTTTTGAAGATCCCTCCATTAAAAAGGTTTGGCACAATTATAGCTTTGATAACCATGTAATTGAGAACTATGGGTTTAAGGTTTCTGGCTTTCATGCCGACACCATGCACATGGCCCGCTTGTGGGATTCATCAAGGCGGTTAAAGGGTGGTTACTCTCTTGAAGCTCTAACACATGACCCAAAGGTCATGTCTGGGGCACCATCATGTGAGGAGAATGACTTGATTGGTAAGATATCAATGAAGACAATATTTGGCAGAAAGAAATTGAAAAAAGATGGTACCGATGGGAAAATAACAATCCTTGACCCAGTTGAAGTGCTACAAAGAGAGGAGAGAAAACCATGGATCTGTTATTCTGCGTTAGATGCAATAAGCACACTAAAGCTTTATGAGAGTATGAAAAACCAACTCTCCAGTAGGCAGTGGGAAATTGATGGAAATCCAGCTCCTGGAACCATGTTTGATTTCTATAAAAAATACTGGCGACCATTTGGCGAGCTCTTAGTTCAGATGGAAACCGAGGGAATGCTGGTTGATCGAGGATATCTAGCTGAAATTGAGAAGTTGGCCAAATCAGAACAAGAGGTTGCTGTGAATAGATTTCGCAAGTGGGCTTCCAGCTTTTGCCCTGATGCTAAGTACATGAATGTGGGAAGTGATGTGCAACTACGCCAGCTTTTATTTGGTGGTACCGTGAACAGCAAGGACTCGAATCAGGCTCTTCCAAATGAGAGGACTTTTAGAGTTCCAAACACTGATAAAGTGATTGAAGAAGGCAAAAGTACAGCTCCAAAATTTCGCAATATCACTCTGCACACAATTGGTGTCAATCTGCCAACTGAGATTTACACAGCAACTGGTTGGCCTTCAGTTAGCGGAGATGCTTTGAAGATTTTAGCTGGCAAGATCTCTTCAGAGTATGATTTTATGGATCCTGCTCCTGATATGGAGTCTGTTGATGATGGAGATTCTTGTGAGACAGTGAGTGATGAATACTTGGGAAAAGAAGAGATTATCTCTGAAAATCTTGACAGGTCTGACTATGGAACAGCTTTGCAAGCATTCGAGTCAGAGGAGAAGGGGAAGGAAGCTTGCCAAGCCATTGCTGCATTATGTCAAGTTTGCTCTATTGATTCGTTGATATCCAACTTCATCCTCCCTTTGCAGGGCAGTAATATAGCTGGCAAAAACAGACGAATTCATTGTTCACTAAATATCAACACAGAAACTGGGCGGTTATCTGCTAGGCGACCAAATTTGCAGAATCAACCAGCTCTTGAAAAGGACCGGTATAAGATCCGCCAGGCATTTGTAGCTGCACCTGGAAATTCTCTCATTGTTGCTGACTATGGACAGCTGGAACTTAGGATTCTTGCTCATCTTTCTAACTGTAAGAGCATGTTGGATGCCTTCAAAGCTGGTGGGGATTTCCATTCAAGGACTGCAATGAACATGTACCAGCATATTCGTGAAGCTGTTGAGAACAAGGAAGTGCTACTTGAGTGGGATCCTCAACCTGGTCAGGATAAACCCCCAGTTCCAATGTTGAAGGATACCTACGGATCTGAGAGAAGGAAAGCTAAAATGCTCAACTTTTCCATTGCATATGGGAAAACTCCAGTTGGGCTTTCCCGGGATTGGAAGGTATCTGTCCAGGATGCAGAGAAAACAGTTGAACTTTGGTACAAAGAAAGAAAAGAAGTGAGAAGGTGGCAAGAAGAACGTAAAAAAGAAGCTAAAGAATTTAGATGCGTTCGAACATTGCTAGGACGGGCACGCTGGTTTCCCTCATTAACTCGTGCTAGCCGTGCTCAAAGAGGACATATTGAAAGGGCTGCTATCAATACTCCAGTGCAGGTTTTTGTACTATCTATCTATCTCGCTATTAATACTCCATGCAGGTTTTTGTCCTATCTATCTCTCTCTCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0003674 GO:0003824 GO:0003887 GO:0004518 GO:0004527 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006264 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0008409 GO:0009058 GO:0009059 GO:0009507 GO:0009536 GO:0009657 GO:0009987 GO:0016043 GO:0016740 GO:0016772 GO:0016779 GO:0016787 GO:0016788 GO:0018130 GO:0019438 GO:0032042 GO:0033258 GO:0033259 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0140097 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03440 map00230 map00240 map01100 map03030 map03410 map03420 map03440

Pfam Domains

Protein Families

DNA_pol_A DNA_pol_A_exo1

Protein Analysis

983

Amino Acids

110.3

Weight (kDa)

8.6

Isoelectric Point (pI)

41.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_A_exo1	PF01612	315 - 477	7.1e-10	3'-5' exonuclease
DNA_pol_A	PF00476	704 - 835	1.9e-30	DNA polymerase family A
DNA_pol_A	PF00476	861 - 959	4e-22	DNA polymerase family A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50840 AT1G50840 AT3G20540 AT3G20540
fragaria_vesca	FvH4_3g22230
malus_domestica	MD11G1221900.v1.1
prunus_persica	Prupe.4G209500_v2.0.a1 Prupe.4G209500_v2.0.a1 Prupe.4G209500_v2.0.a1
pyrus_communis	pycom11g19570
rosa_chinensis	RchiOBHm_Chr5g0038301
rosa_laevigata	RLG00000033845
rosa_multiflora	Rmu_co8325491.1_g000001
rosa_roxburghii	Rroxscaffold_1G00042720
rosa_rugosa	Rorug05G0171900
rosa_samantha	Rh5AG259200 Rh5BG263000 Rh5CG295400 Rh5DG272000
rosa_wichuraiana	Rw5G024260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1202
Acc36I	ACCTGC	2 cut(s)	2866, 2910
Acc65I	GGTACC	2 cut(s)	1298, 1709
AccB1I	GGYRCC	4 cut(s)	165, 1216, 1298, 1709
AccB7I	CCANNNNNTGG	1 cut(s)	1469
AciI	CCGC	7 cut(s)	254, 987, 1135, 1156, 1896, 2254, 2320
AclWI	GGATC	8 cut(s)	1022, 1373, 1946, 1959, 2311, 2522, 2535, 2590
AcoI	YGGCCR	2 cut(s)	855, 1587
AcsI	RAATTY	6 cut(s)	1007, 1814, 2217, 2275, 2347, 2762
AcuI	CTGAAG	6 cut(s)	102, 234, 339, 895, 1872, 1917
AcyI	GRCGYC	1 cut(s)	166
AdeI	CACNNNGTG	1 cut(s)	404
AfaI	GTAC	8 cut(s)	711, 1300, 1661, 1711, 1804, 2477, 2705, 2886
AflIII	ACRYGT	1 cut(s)	2472
AgeI	ACCGGT	1 cut(s)	2307
AjnI	CCWGG	7 cut(s)	180, 242, 1468, 2322, 2341, 2536, 2673
AleI	CACNNNNGTG	1 cut(s)	851
Alw21I	GWGCWC	2 cut(s)	1521, 2833
Alw26I	GTCTC	3 cut(s)	389, 784, 1991
AlwI	GGATC	8 cut(s)	1022, 1373, 1946, 1959, 2311, 2522, 2535, 2590
AlwNI	CAGNNNCTG	3 cut(s)	1469, 1877, 2249
Ama87I	CYCGRG	1 cut(s)	2652
AoxI	GGCC	7 cut(s)	41, 102, 578, 855, 1131, 1587, 1883
ApaI	GGGCCC	1 cut(s)	106
ApeKI	GCWGC	6 cut(s)	185, 251, 698, 2126, 2336, 2855
ApoI	RAATTY	6 cut(s)	1007, 1814, 2217, 2275, 2347, 2762
ArsI	GACNNNNNNTTYG	2 cut(s)	1193, 1225
AseI	ATTAAT	1 cut(s)	2907
AsiGI	ACCGGT	1 cut(s)	2307
Asp700I	GAANNNNTTC	1 cut(s)	726
Asp718I	GGTACC	2 cut(s)	1298, 1709
AspLEI	GCGC	1 cut(s)	168
AspS9I	GGNCC	8 cut(s)	102, 103, 521, 578, 626, 926, 1132, 2305
AsuC2I	CCSGG	2 cut(s)	2653, 2654
AsuHPI	GGTGA	4 cut(s)	394, 416, 511, 827
AsuII	TTCGAA	1 cut(s)	2776
AsuNHI	GCTAGC	1 cut(s)	2822
AvaI	CYCGRG	1 cut(s)	2652
AvaII	GGWCC	4 cut(s)	521, 626, 926, 2305
AxyI	CCTNAGG	2 cut(s)	170, 266
BaeGI	GKGCMC	3 cut(s)	106, 1219, 2797
BalI	TGGCCA	1 cut(s)	1589
BamHI	GGATCC	2 cut(s)	1951, 2527
BanI	GGYRCC	4 cut(s)	165, 1216, 1298, 1709
BanII	GRGCYC	2 cut(s)	106, 1521
BarI	GAAGNNNNNNTAC	2 cut(s)	2561, 2593
BauI	CACGAG	1 cut(s)	2817
BbsI	GAAGAC	4 cut(s)	78, 87, 599, 1268
Bbv12I	GWGCWC	2 cut(s)	1521, 2833
BbvI	GCAGC	6 cut(s)	172, 238, 685, 2113, 2323, 2842
BccI	CCATC	9 cut(s)	367, 512, 974, 1228, 1289, 1298, 1448, 1525, 1976
BceAI	ACGGC	1 cut(s)	2811
BciT130I	CCWGG	7 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2675
BciVI	GTATCC	1 cut(s)	2566
BcnI	CCSGG	2 cut(s)	2653, 2654
BcoDI	GTCTC	3 cut(s)	389, 784, 1991
BfaI	CTAG	8 cut(s)	99, 234, 486, 630, 1568, 2265, 2786, 2823
BfmI	CTRYAG	2 cut(s)	468, 885
BfoI	RGCGCY	1 cut(s)	169
BfuAI	ACCTGC	2 cut(s)	2866, 2910
BfuI	GTATCC	1 cut(s)	2566
BglII	AGATCT	2 cut(s)	724, 1926
BisI	GCNGC	7 cut(s)	186, 252, 699, 988, 2127, 2337, 2856
BlsI	GCNGC	7 cut(s)	187, 253, 700, 989, 2128, 2338, 2857
Bme1390I	CCNGG	9 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2653, 2654, 2675
Bme18I	GGWCC	4 cut(s)	521, 626, 926, 2305
BmeT110I	CYCGRG	1 cut(s)	2652
BmgT120I	GGNCC	8 cut(s)	102, 103, 521, 578, 626, 926, 1132, 2305
BmiI	GGNNCC	9 cut(s)	104, 167, 579, 1218, 1300, 1474, 1711, 1953, 2529
BmrFI	CCNGG	9 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2653, 2654, 2675
BmrI	ACTGGG	3 cut(s)	1322, 2259, 2549
BmsI	GCATC	9 cut(s)	795, 835, 969, 1375, 1642, 1891, 2416, 2668, 2759
BmtI	GCTAGC	1 cut(s)	2826
BmuI	ACTGGG	3 cut(s)	1322, 2259, 2549
BpiI	GAAGAC	4 cut(s)	78, 87, 599, 1268
BplI	GAGNNNNNCTC	2 cut(s)	2021, 2053
BpmI	CTGGAG	4 cut(s)	265, 1417, 2622, 2853
Bpu14I	TTCGAA	1 cut(s)	2776
BpuEI	CTTGAG	1 cut(s)	2540
BpuMI	CCSGG	2 cut(s)	2653, 2654
BsaBI	GATNNNNATC	1 cut(s)	1458
BsaHI	GRCGYC	1 cut(s)	166
BsaJI	CCNNGG	4 cut(s)	573, 1361, 1539, 2652
BsaWI	WCCGGW	1 cut(s)	2307
BsaXI	ACNNNNNCTCC	2 cut(s)	1415, 1445
Bse118I	RCCGGY	2 cut(s)	644, 2307
Bse1I	ACTGG	9 cut(s)	707, 1328, 1434, 1505, 1882, 2254, 2555, 2639, 2870
Bse21I	CCTNAGG	2 cut(s)	170, 266
Bse3DI	GCAATG	4 cut(s)	2121, 2472, 2619, 2780
Bse8I	GATNNNNATC	1 cut(s)	1458
BseBI	CCWGG	7 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2675
BseDI	CCNNGG	4 cut(s)	573, 1361, 1539, 2652
BseGI	GGATG	5 cut(s)	810, 976, 2172, 2431, 2683
BseJI	GATNNNNATC	1 cut(s)	1458
BseMI	GCAATG	4 cut(s)	2121, 2472, 2619, 2780
BseMII	CTCAG	4 cut(s)	257, 521, 1851, 2577
BseNI	ACTGG	9 cut(s)	707, 1328, 1434, 1505, 1882, 2254, 2555, 2639, 2870
BseRI	GAGGAG	4 cut(s)	7, 1244, 1364, 2108
BseSI	GKGCMC	3 cut(s)	106, 1219, 2797
BseXI	GCAGC	6 cut(s)	172, 238, 685, 2113, 2323, 2842
BsgI	GTGCAG	3 cut(s)	1817, 2322, 2894
BshFI	GGCC	7 cut(s)	43, 104, 580, 857, 1133, 1589, 1885
BshNI	GGYRCC	4 cut(s)	165, 1216, 1298, 1709
BshTI	ACCGGT	1 cut(s)	2307
BsiHKAI	GWGCWC	2 cut(s)	1521, 2833
BsiHKCI	CYCGRG	1 cut(s)	2652
BsiSI	CCGG	3 cut(s)	645, 2308, 2653
BsmAI	GTCTC	3 cut(s)	389, 784, 1991
BsmI	GAATGC	2 cut(s)	1551, 2081
BsnI	GGCC	7 cut(s)	43, 104, 580, 857, 1133, 1589, 1885
BsoBI	CYCGRG	1 cut(s)	2652
Bsp119I	TTCGAA	1 cut(s)	2776
Bsp120I	GGGCCC	1 cut(s)	102
Bsp1286I	GDGCHC	5 cut(s)	106, 1219, 1521, 2797, 2833
Bsp19I	CCATGG	1 cut(s)	1361
BspACI	CCGC	7 cut(s)	254, 987, 1135, 1156, 1896, 2254, 2320
BspANI	GGCC	7 cut(s)	43, 104, 580, 857, 1133, 1589, 1885
BspCNI	CTCAG	4 cut(s)	258, 522, 1852, 2578
BspLI	GGNNCC	9 cut(s)	104, 167, 579, 1218, 1300, 1474, 1711, 1953, 2529
BspMI	ACCTGC	2 cut(s)	2866, 2910
BspOI	GCTAGC	1 cut(s)	2826
BspPI	GGATC	8 cut(s)	1022, 1373, 1946, 1959, 2311, 2522, 2535, 2590
BspQI	GCTCTTC	1 cut(s)	1746
BspT104I	TTCGAA	1 cut(s)	2776
BspT107I	GGYRCC	4 cut(s)	165, 1216, 1298, 1709
BsrDI	GCAATG	4 cut(s)	2121, 2472, 2619, 2780
BsrFI	RCCGGY	2 cut(s)	644, 2307
BsrI	ACTGG	9 cut(s)	707, 1328, 1434, 1505, 1882, 2254, 2555, 2639, 2870
BssAI	RCCGGY	2 cut(s)	644, 2307
BssECI	CCNNGG	4 cut(s)	573, 1361, 1539, 2652
BssNI	GRCGYC	1 cut(s)	166
BssSI	CACGAG	1 cut(s)	2817
BssT1I	CCWWGG	2 cut(s)	573, 1361
Bst2BI	CACGAG	1 cut(s)	2817
Bst2UI	CCWGG	7 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2675
Bst4CI	ACNGT	6 cut(s)	362, 434, 1714, 2002, 2411, 2692
Bst6I	CTCTTC	3 cut(s)	1746, 1936, 2022
BstACI	GRCGYC	1 cut(s)	166
BstBI	TTCGAA	1 cut(s)	2776
BstDEI	CTNAG	9 cut(s)	170, 266, 530, 809, 1522, 1656, 1860, 2384, 2586
BstDSI	CCRYGG	1 cut(s)	1361
BstF5I	GGATG	5 cut(s)	810, 976, 2172, 2431, 2683
BstH2I	RGCGCY	1 cut(s)	169
BstHHI	GCGC	1 cut(s)	168
BstMAI	GTCTC	3 cut(s)	389, 784, 1991
BstMWI	GCNNNNNNNGC	8 cut(s)	248, 1385, 2078, 2117, 2126, 2333, 2435, 2828
BstNI	CCWGG	7 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2675
BstNSI	RCATGY	2 cut(s)	2422, 2476
BstSCI	CCNGG	9 cut(s)	180, 242, 1468, 2322, 2341, 2536, 2651, 2652, 2673
BstSFI	CTRYAG	2 cut(s)	468, 885
BstSLI	GKGCMC	3 cut(s)	106, 1219, 2797
BstV1I	GCAGC	6 cut(s)	172, 238, 685, 2113, 2323, 2842
BstV2I	GAAGAC	4 cut(s)	78, 87, 599, 1268
BstX2I	RGATCY	8 cut(s)	724, 1027, 1365, 1926, 1951, 2316, 2527, 2582
BstXI	CCANNNNNNTGG	1 cut(s)	1129
BstYI	RGATCY	8 cut(s)	724, 1027, 1365, 1926, 1951, 2316, 2527, 2582
Bsu36I	CCTNAGG	2 cut(s)	170, 266
BsuI	GTATCC	1 cut(s)	2566
BsuRI	GGCC	7 cut(s)	43, 104, 580, 857, 1133, 1589, 1885
BtgI	CCRYGG	1 cut(s)	1361
BtsCI	GGATG	5 cut(s)	810, 976, 2172, 2431, 2683
BtsI	GCAGTG	1 cut(s)	1448
BtsIMutI	CAGTG	4 cut(s)	1448, 1773, 2007, 2877
BveI	ACCTGC	2 cut(s)	2866, 2910
CaiI	CAGNNNCTG	3 cut(s)	1469, 1877, 2249
CfoI	GCGC	1 cut(s)	168
Cfr10I	RCCGGY	2 cut(s)	644, 2307
Cfr13I	GGNCC	8 cut(s)	102, 103, 521, 578, 626, 926, 1132, 2305
Cfr9I	CCCGGG	1 cut(s)	2652
CsiI	ACCWGGT	1 cut(s)	2536
Csp6I	GTAC	8 cut(s)	710, 1299, 1660, 1710, 1803, 2476, 2704, 2885
CspAI	ACCGGT	1 cut(s)	2307
CspCI	CAANNNNNGTGG	2 cut(s)	2422, 2457
CviQI	GTAC	8 cut(s)	710, 1299, 1660, 1710, 1803, 2476, 2704, 2885
DdeI	CTNAG	9 cut(s)	170, 266, 530, 809, 1522, 1656, 1860, 2384, 2586
DinI	GGCGCC	1 cut(s)	167
DraIII	CACNNNGTG	1 cut(s)	404
DrdI	GACNNNNNNGTC	1 cut(s)	1202
DseDI	GACNNNNNNGTC	1 cut(s)	1202
EaeI	YGGCCR	2 cut(s)	855, 1587
Eam1104I	CTCTTC	3 cut(s)	1746, 1936, 2022
EarI	CTCTTC	3 cut(s)	1746, 1936, 2022
EciI	GGCGGA	1 cut(s)	2309
Ecl136II	GAGCTC	1 cut(s)	1519
Eco130I	CCWWGG	2 cut(s)	573, 1361
Eco147I	AGGCCT	1 cut(s)	43
Eco24I	GRGCYC	2 cut(s)	106, 1521
Eco32I	GATATC	3 cut(s)	1254, 1565, 2163
Eco47I	GGWCC	4 cut(s)	521, 626, 926, 2305
Eco53kI	GAGCTC	1 cut(s)	1519
Eco57I	CTGAAG	6 cut(s)	102, 234, 339, 895, 1872, 1917
Eco81I	CCTNAGG	2 cut(s)	170, 266
Eco88I	CYCGRG	1 cut(s)	2652
EcoICRI	GAGCTC	1 cut(s)	1519
EcoO109I	RGGNCCY	2 cut(s)	102, 103
EcoRI	GAATTC	1 cut(s)	2217
EcoRII	CCWGG	7 cut(s)	180, 242, 1468, 2322, 2341, 2536, 2673
EcoRV	GATATC	3 cut(s)	1254, 1565, 2163
EcoT14I	CCWWGG	2 cut(s)	573, 1361
EcoT22I	ATGCAT	1 cut(s)	962
EcoT38I	GRGCYC	2 cut(s)	106, 1521
EgeI	GGCGCC	1 cut(s)	167
EheI	GGCGCC	1 cut(s)	167
ErhI	CCWWGG	2 cut(s)	573, 1361
FalI	AAGNNNNNCTT	2 cut(s)	2502, 2534
FauI	CCCGC	1 cut(s)	1142
FauNDI	CATATG	1 cut(s)	2626
Fnu4HI	GCNGC	7 cut(s)	186, 252, 699, 988, 2127, 2337, 2856
FokI	GGATG	5 cut(s)	817, 983, 2159, 2438, 2690
FriOI	GRGCYC	2 cut(s)	106, 1521
Fsp4HI	GCNGC	7 cut(s)	186, 252, 699, 988, 2127, 2337, 2856
FspBI	CTAG	8 cut(s)	99, 234, 486, 630, 1568, 2265, 2786, 2823
GlaI	GCGC	1 cut(s)	167
GluI	GCNGC	7 cut(s)	186, 252, 699, 988, 2127, 2337, 2856
GsuI	CTGGAG	4 cut(s)	265, 1417, 2622, 2853
HaeII	RGCGCY	1 cut(s)	169
HaeIII	GGCC	7 cut(s)	43, 104, 580, 857, 1133, 1589, 1885
HapII	CCGG	3 cut(s)	645, 2308, 2653
HhaI	GCGC	1 cut(s)	168
Hin1I	GRCGYC	1 cut(s)	166
Hin6I	GCGC	1 cut(s)	166
HinP1I	GCGC	1 cut(s)	166
HindIII	AAGCTT	2 cut(s)	1403, 2109
HpaII	CCGG	3 cut(s)	645, 2308, 2653
HphI	GGTGA	4 cut(s)	394, 416, 511, 827
Hpy166II	GTNNAC	4 cut(s)	421, 430, 1717, 2228
Hpy8I	GTNNAC	4 cut(s)	421, 430, 1717, 2228
HpyCH4III	ACNGT	6 cut(s)	362, 434, 1714, 2002, 2411, 2692
HpyCH4IV	ACGT	1 cut(s)	2743
HpyF10VI	GCNNNNNNNGC	8 cut(s)	248, 1385, 2078, 2117, 2126, 2333, 2435, 2828
HpyF3I	CTNAG	9 cut(s)	170, 266, 530, 809, 1522, 1656, 1860, 2384, 2586
HpySE526I	ACGT	1 cut(s)	2743
Hsp92I	GRCGYC	1 cut(s)	166
HspAI	GCGC	1 cut(s)	166
KasI	GGCGCC	1 cut(s)	165
KpnI	GGTACC	2 cut(s)	1302, 1713
LguI	GCTCTTC	1 cut(s)	1746
LmnI	GCTCC	4 cut(s)	1018, 1471, 1813, 1963
Lsp1109I	GCAGC	6 cut(s)	172, 238, 685, 2113, 2323, 2842
LweI	GCATC	9 cut(s)	795, 835, 969, 1375, 1642, 1891, 2416, 2668, 2759
MabI	ACCWGGT	1 cut(s)	2536
MaeI	CTAG	8 cut(s)	99, 234, 486, 630, 1568, 2265, 2786, 2823
MaeII	ACGT	1 cut(s)	2743
MaeIII	GTNAC	4 cut(s)	256, 382, 842, 1169
MfeI	CAATTG	1 cut(s)	1839
MflI	RGATCY	8 cut(s)	724, 1027, 1365, 1926, 1951, 2316, 2527, 2582
MhlI	GDGCHC	5 cut(s)	106, 1219, 1521, 2797, 2833
MlsI	TGGCCA	1 cut(s)	1589
MluNI	TGGCCA	1 cut(s)	1589
Mly113I	GGCGCC	1 cut(s)	166
MlyI	GAGTC	5 cut(s)	106, 224, 1721, 1979, 2096
MmeI	TCCRAC	3 cut(s)	249, 2190, 2403
Mox20I	TGGCCA	1 cut(s)	1589
Mph1103I	ATGCAT	1 cut(s)	962
MroXI	GAANNNNTTC	1 cut(s)	726
MscI	TGGCCA	1 cut(s)	1589
MseI	TTAA	8 cut(s)	567, 741, 870, 1038, 1095, 1160, 2813, 2907
MslI	CAYNNNNRTG	7 cut(s)	840, 851, 965, 1127, 1222, 1667, 2487
Msp20I	TGGCCA	1 cut(s)	1589
MspA1I	CMGCKG	1 cut(s)	2377
MspI	CCGG	3 cut(s)	645, 2308, 2653
MspR9I	CCNGG	9 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2653, 2654, 2675
MunI	CAATTG	1 cut(s)	1839
Mva1269I	GAATGC	2 cut(s)	1551, 2081
MvaI	CCWGG	7 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2675
MwoI	GCNNNNNNNGC	8 cut(s)	248, 1385, 2078, 2117, 2126, 2333, 2435, 2828
NarI	GGCGCC	1 cut(s)	166
NciI	CCSGG	2 cut(s)	2653, 2654
NcoI	CCATGG	1 cut(s)	1361
NdeI	CATATG	1 cut(s)	2626
NheI	GCTAGC	1 cut(s)	2822
NlaIV	GGNNCC	9 cut(s)	104, 167, 579, 1218, 1300, 1474, 1711, 1953, 2529
NmeAIII	GCCGAG	1 cut(s)	883
NmuCI	GTSAC	2 cut(s)	382, 842
NsiI	ATGCAT	1 cut(s)	962
NspI	RCATGY	2 cut(s)	2422, 2476
NspV	TTCGAA	1 cut(s)	2776
OliI	CACNNNNGTG	1 cut(s)	851
PceI	AGGCCT	1 cut(s)	43
PciI	ACATGT	1 cut(s)	2472
PciSI	GCTCTTC	1 cut(s)	1746
PcsI	WCGNNNNNNNCGW	1 cut(s)	2824
PctI	GAATGC	2 cut(s)	1551, 2081
PdmI	GAANNNNTTC	1 cut(s)	726
PfeI	GAWTC	8 cut(s)	620, 689, 1145, 1732, 1988, 2153, 2284, 2389
PflMI	CCANNNNNTGG	1 cut(s)	1469
PfoI	TCCNGGA	2 cut(s)	1468, 2673
PinAI	ACCGGT	1 cut(s)	2307
PkrI	GCNGC	7 cut(s)	187, 253, 700, 989, 2128, 2338, 2857
PleI	GAGTC	5 cut(s)	106, 224, 1721, 1978, 2095
PluTI	GGCGCC	1 cut(s)	169
PpsI	GAGTC	5 cut(s)	106, 224, 1721, 1978, 2095
PscI	ACATGT	1 cut(s)	2472
PshBI	ATTAAT	1 cut(s)	2907
Psp124BI	GAGCTC	1 cut(s)	1521
Psp6I	CCWGG	7 cut(s)	180, 242, 1468, 2322, 2341, 2536, 2673
PspGI	CCWGG	7 cut(s)	180, 242, 1468, 2322, 2341, 2536, 2673
PspN4I	GGNNCC	9 cut(s)	104, 167, 579, 1218, 1300, 1474, 1711, 1953, 2529
PspOMI	GGGCCC	1 cut(s)	102
PspPI	GGNCC	8 cut(s)	102, 103, 521, 578, 626, 926, 1132, 2305
PstNI	CAGNNNCTG	3 cut(s)	1469, 1877, 2249
PsuI	RGATCY	8 cut(s)	724, 1027, 1365, 1926, 1951, 2316, 2527, 2582
PvuII	CAGCTG	1 cut(s)	2377
RsaI	GTAC	8 cut(s)	711, 1300, 1661, 1711, 1804, 2477, 2705, 2886
RsaNI	GTAC	8 cut(s)	710, 1299, 1660, 1710, 1803, 2476, 2704, 2885
RseI	CAYNNNNRTG	7 cut(s)	840, 851, 965, 1127, 1222, 1667, 2487
SacI	GAGCTC	1 cut(s)	1521
SapI	GCTCTTC	1 cut(s)	1746
SaqAI	TTAA	8 cut(s)	567, 741, 870, 1038, 1095, 1160, 2813, 2907
SatI	GCNGC	7 cut(s)	186, 252, 699, 988, 2127, 2337, 2856
Sau96I	GGNCC	8 cut(s)	102, 103, 521, 578, 626, 926, 1132, 2305
SchI	GAGTC	5 cut(s)	106, 224, 1721, 1979, 2096
ScrFI	CCNGG	9 cut(s)	182, 244, 1470, 2324, 2343, 2538, 2653, 2654, 2675
SduI	GDGCHC	5 cut(s)	106, 1219, 1521, 2797, 2833
SexAI	ACCWGGT	1 cut(s)	2536
SfaNI	GCATC	9 cut(s)	795, 835, 969, 1375, 1642, 1891, 2416, 2668, 2759
SfcI	CTRYAG	2 cut(s)	468, 885
SfoI	GGCGCC	1 cut(s)	167
SfuI	TTCGAA	1 cut(s)	2776
SinI	GGWCC	4 cut(s)	521, 626, 926, 2305
SmaI	CCCGGG	1 cut(s)	2654
SmiMI	CAYNNNNRTG	7 cut(s)	840, 851, 965, 1127, 1222, 1667, 2487
SmlI	CTYRAG	1 cut(s)	2519
SmoI	CTYRAG	1 cut(s)	2519
SseBI	AGGCCT	1 cut(s)	43
SsiI	CCGC	7 cut(s)	254, 987, 1135, 1156, 1896, 2254, 2320
SspDI	GGCGCC	1 cut(s)	165
SspI	AATATT	1 cut(s)	1269
SspMI	CTAG	8 cut(s)	99, 234, 486, 630, 1568, 2265, 2786, 2823
SstI	GAGCTC	1 cut(s)	1521
StuI	AGGCCT	1 cut(s)	43
StyD4I	CCNGG	9 cut(s)	180, 242, 1468, 2322, 2341, 2536, 2651, 2652, 2673
StyI	CCWWGG	2 cut(s)	573, 1361
TaaI	ACNGT	6 cut(s)	362, 434, 1714, 2002, 2411, 2692
TaiI	ACGT	1 cut(s)	2746
TaqI	TCGA	4 cut(s)	1558, 1730, 2085, 2776
TatI	WGTACW	4 cut(s)	709, 1659, 1802, 2884
TauI	GCSGC	1 cut(s)	990
TfiI	GAWTC	8 cut(s)	620, 689, 1145, 1732, 1988, 2153, 2284, 2389
Tru1I	TTAA	8 cut(s)	567, 741, 870, 1038, 1095, 1160, 2813, 2907
Tru9I	TTAA	8 cut(s)	567, 741, 870, 1038, 1095, 1160, 2813, 2907
TscAI	CASTG	4 cut(s)	1448, 1780, 2007, 2877
TseFI	GTSAC	2 cut(s)	382, 842
TseI	GCWGC	6 cut(s)	185, 251, 698, 2126, 2336, 2855
Tsp45I	GTSAC	2 cut(s)	382, 842
TspGWI	ACGGA	1 cut(s)	2595
TspMI	CCCGGG	1 cut(s)	2652
TspRI	CASTG	4 cut(s)	1448, 1780, 2007, 2877
Van91I	CCANNNNNTGG	1 cut(s)	1469
VpaK11BI	GGWCC	4 cut(s)	521, 626, 926, 2305
VspI	ATTAAT	1 cut(s)	2907
XapI	RAATTY	6 cut(s)	1007, 1814, 2217, 2275, 2347, 2762
XbaI	TCTAGA	1 cut(s)	233
XceI	RCATGY	2 cut(s)	2422, 2476
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1700
XmaI	CCCGGG	1 cut(s)	2652
XmnI	GAANNNNTTC	1 cut(s)	726
XspI	CTAG	8 cut(s)	99, 234, 486, 630, 1568, 2265, 2786, 2823
Zsp2I	ATGCAT	1 cut(s)	962

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.