AT3G20570

early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014590
Physical Location & Seq
Reverse (-)
7186522 .. 7187521
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20570.1

Sequence Viewer

Length: 612 bp
ATGGCACGAAACCTAAAGAGCATGATGCTTTGTGGGTTTGGTCTCTTGTGTTTTCTTATGATCGTTGACCGAGCCTACGCTAGAGAGTTTACGGTCGGTGGTGCAACAGGTTGGACCGTCCCTTCTGGTTCTCAAGTGTACAGTCAATGGGCAGAACAGAGCAGATTCCAAATTGGTGACTCTCTGTTGTTCGTCTACCAATCAAACCAAGATTCAGTGCTCCAAGTCACAAGAGACGCTTACGACAGCTGCAACACGGACTCACCAACCGCTAAATTCGCCGATGGCAAAACTTCTGTCACGCTGAACCACTCTGGGCCATACTATTTCATTAGTGGAAACAAAGACAACTGCAAGAAAAACGAGAAGCTTGTGGTCATCGTCATGGCTGATAGAAGCGGCAACAAAAACACCGCATCATCACCGCCGTCCCCAGCCCCAGCTCCCTCAGGAGAATCCGCTCCTTCTCCACCTGTCTCGGGAACCTTTGAGATGACCCCAGCACCGACGCCTACTACTTCTGAAGACACTCCAAACAGCGCAGCTTCTTCCTTATCATTTGTCGCTGCTCTTCTTGGGGCTGCTCTAGCTTCCACTCTATTCCTACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

203

Amino Acids

21.51

Weight (kDa)

5.62

Isoelectric Point (pI)

50.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 38 - 120 5.2e-27 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20570
fragaria_vesca FvH4_3g21980
malus_domestica MD03G1209600.v1.1 MD11G1224400.v1.1
prunus_persica Prupe.4G205500_v2.0.a1
pyrus_communis pycom03g15840 pycom11g19810
rosa_chinensis RchiOBHm_Chr5g0037831
rosa_laevigata RLG00000033815
rosa_multiflora Rmu_sc0005416.1_g000014
rosa_roxburghii Rroxscaffold_1G00043090
rosa_rugosa Rorug05G0168000
rosa_samantha Rh5AG256800 Rh5BG259200 Rh5CG292200 Rh5DG269100
rosa_wichuraiana Rw5G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 461
AccI GTMKAC 1 cut(s) 195
AciI CCGC 5 cut(s) 270, 399, 414, 425, 459
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 543
AcyI GRCGYC 1 cut(s) 509
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 479
AluBI AGCT 5 cut(s) 249, 370, 443, 545, 590
AluI AGCT 5 cut(s) 249, 370, 443, 545, 590
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 3 cut(s) 47, 228, 481
Ama87I CYCGRG 1 cut(s) 478
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 4 cut(s) 249, 542, 566, 581
ApoI RAATTY 1 cut(s) 275
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 114, 317
AsuHPI GGTGA 3 cut(s) 188, 255, 414
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 1 cut(s) 114
AxyI CCTNAGG 1 cut(s) 448
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 4 cut(s) 236, 553, 554, 568
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 412
BcoDI GTCTC 3 cut(s) 47, 228, 481
BfaI CTAG 2 cut(s) 81, 587
BisI GCNGC 5 cut(s) 250, 400, 543, 567, 582
BlsI GCNGC 5 cut(s) 251, 401, 544, 568, 583
Bme18I GGWCC 1 cut(s) 114
BmeT110I CYCGRG 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 114, 317
BmiI GGNNCC 1 cut(s) 484
BmsI GCATC 2 cut(s) 15, 425
BpiI GAAGAC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 47
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse21I CCTNAGG 1 cut(s) 448
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 462
BseXI GCAGC 4 cut(s) 236, 553, 554, 568
BseYI CCCAGC 3 cut(s) 433, 439, 499
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 1 cut(s) 319
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 478
BslFI GGGAC 2 cut(s) 104, 415
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 3 cut(s) 47, 228, 481
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 104, 415
BsnI GGCC 1 cut(s) 319
Bso31I GGTCTC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 478
Bsp1286I GDGCHC 1 cut(s) 222
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 1 cut(s) 60
BspACI CCGC 5 cut(s) 270, 399, 414, 425, 459
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 1 cut(s) 461
BspLI GGNNCC 1 cut(s) 484
BspQI GCTCTTC 1 cut(s) 576
BspTNI GGTCTC 1 cut(s) 47
BsrBI CCGCTC 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 138
BssMI GATC 1 cut(s) 60
BssNI GRCGYC 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 94, 118, 143
Bst6I CTCTTC 1 cut(s) 576
BstACI GRCGYC 1 cut(s) 509
BstAUI TGTACA 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 448
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 1 cut(s) 63
BstMAI GTCTC 3 cut(s) 47, 228, 481
BstMBI GATC 1 cut(s) 60
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 2 cut(s) 278, 587
BstV1I GCAGC 4 cut(s) 236, 553, 554, 568
BstV2I GAAGAC 1 cut(s) 531
Bsu36I CCTNAGG 1 cut(s) 448
BsuRI GGCC 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 222
CfoI GCGC 1 cut(s) 542
Cfr13I GGNCC 2 cut(s) 114, 317
CseI GACGC 2 cut(s) 245, 517
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 22, 385
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 448
DpnI GATC 1 cut(s) 62
DpnII GATC 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 576
EarI CTCTTC 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 543
Eco81I CCTNAGG 1 cut(s) 448
Eco88I CYCGRG 1 cut(s) 478
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 2 cut(s) 25, 388
FaiI YATR 4 cut(s) 23, 59, 322, 386
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FaqI GGGAC 2 cut(s) 104, 415
FatI CATG 2 cut(s) 21, 384
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 250, 400, 543, 567, 582
Fsp4HI GCNGC 5 cut(s) 250, 400, 543, 567, 582
FspBI CTAG 2 cut(s) 81, 587
GlaI GCGC 1 cut(s) 541
GluI GCNGC 5 cut(s) 250, 400, 543, 567, 582
GsaI CCCAGC 3 cut(s) 437, 443, 503
HaeIII GGCC 1 cut(s) 319
HgaI GACGC 2 cut(s) 245, 517
HhaI GCGC 1 cut(s) 542
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 2 cut(s) 25, 388
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 5 cut(s) 165, 179, 212, 260, 455
HphI GGTGA 3 cut(s) 188, 255, 414
Hpy166II GTNNAC 4 cut(s) 67, 90, 139, 196
Hpy188I TCNGA 1 cut(s) 523
Hpy188III TCNNGA 2 cut(s) 450, 480
Hpy8I GTNNAC 4 cut(s) 67, 90, 139, 196
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 2 cut(s) 132, 474
HpyCH4III ACNGT 3 cut(s) 94, 118, 143
HpyCH4V TGCA 3 cut(s) 104, 252, 354
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 587
HpyF3I CTNAG 1 cut(s) 448
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 2 cut(s) 25, 388
HspAI GCGC 1 cut(s) 540
Kzo9I GATC 1 cut(s) 60
LguI GCTCTTC 1 cut(s) 576
LmnI GCTCC 3 cut(s) 225, 448, 466
LpnPI CCDG 8 cut(s) 93, 111, 300, 435, 447, 453, 486, 513
Lsp1109I GCAGC 4 cut(s) 236, 553, 554, 568
LweI GCATC 2 cut(s) 15, 425
MaeI CTAG 2 cut(s) 81, 587
MaeIII GTNAC 3 cut(s) 176, 226, 298
MalI GATC 1 cut(s) 62
MbiI CCGCTC 1 cut(s) 461
MboI GATC 1 cut(s) 60
MboII GAAGA 3 cut(s) 536, 540, 563
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 2 cut(s) 171, 275
MlyI GAGTC 2 cut(s) 173, 254
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 1 cut(s) 457
MslI CAYNNNNRTG 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 249
MwoI GCNNNNNNNGC 2 cut(s) 278, 587
NdeII GATC 1 cut(s) 60
NlaIII CATG 2 cut(s) 25, 388
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 3 cut(s) 176, 226, 298
PciSI GCTCTTC 1 cut(s) 576
PfeI GAWTC 3 cut(s) 165, 212, 455
PkrI GCNGC 5 cut(s) 251, 401, 544, 568, 583
PleI GAGTC 2 cut(s) 173, 254
PpsI GAGTC 2 cut(s) 173, 254
PspFI CCCAGC 3 cut(s) 433, 439, 499
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 2 cut(s) 114, 317
PvuII CAGCTG 1 cut(s) 249
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
RseI CAYNNNNRTG 1 cut(s) 383
SapI GCTCTTC 1 cut(s) 576
SatI GCNGC 5 cut(s) 250, 400, 543, 567, 582
Sau3AI GATC 1 cut(s) 60
Sau96I GGNCC 2 cut(s) 114, 317
SchI GAGTC 2 cut(s) 173, 254
SduI GDGCHC 1 cut(s) 222
SetI ASST 9 cut(s) 15, 112, 251, 372, 445, 475, 488, 547, 592
SfaNI GCATC 2 cut(s) 15, 425
SinI GGWCC 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
Sse9I AATT 2 cut(s) 171, 275
SsiI CCGC 5 cut(s) 270, 399, 414, 425, 459
SspMI CTAG 2 cut(s) 81, 587
TaaI ACNGT 3 cut(s) 94, 118, 143
TaqII GACCGA 1 cut(s) 84
TasI AATT 2 cut(s) 171, 275
TatI WGTACW 1 cut(s) 138
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 3 cut(s) 165, 212, 455
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 3 cut(s) 176, 226, 298
TseI GCWGC 4 cut(s) 249, 542, 566, 581
Tsp45I GTSAC 3 cut(s) 176, 226, 298
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 2 cut(s) 81, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.