RLG00000033815

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014590
Physical Location & Seq
Forward (+)
32536862 .. 32537928
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033815

Sequence Viewer

Length: 618 bp
ATGGCCAACTCTAATATTCTAAGAGCATATAATAAAAGCATTGCATTTCCTGTATTCGGTCTTCTTTGTCTATCGCTGTTACTGCAGAAGAGTGGTGCAAATGAGTTTACAGTAGCTTGGACTGTTCCTGATGCATCAACTCATTATAGTCAATGGGCAGAGACCAACAGATTTCAAATTGGTGACTCCATAGTGTTCAACTATTCTCCTGGCCAAGACTCAGTGCTTCGCGTCGGCCAGGATGACTACACAAATTGCAATACTGATGCTCCAACAGCTGAGAAATATACCGATGGCCACACTACCTACAAGTTCAACCAATCTGGGCCATTCTACTTCATGAGTGGGAACAAAGATAACTGTCTCAAAAATGAGAAGATGGTTGTGATTGTCTTGGCAGACAGGAACCAAACAAGCAATGCAACTACTCCATCTCCTGCACCTTCTGGCGAAGCCTCTCCACCTCCCCCTTCACCAGGGACGCTCCAGATCAACCCAACTCCATCTCCCGAAAGTGATCAAATCCCTCCTCCTCCTAGCGGTGCTTCTTCAATTTTCATCAGTTTAGTTGGATCCATTGGAGCAGTTGCTGCGTATACATCATTTTTATTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

206

Amino Acids

22.01

Weight (kDa)

4.85

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 123 2.1e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20570
fragaria_vesca FvH4_3g21980
malus_domestica MD03G1209600.v1.1 MD11G1224400.v1.1
prunus_persica Prupe.4G205500_v2.0.a1
pyrus_communis pycom03g15840 pycom11g19810
rosa_chinensis RchiOBHm_Chr5g0037831
rosa_laevigata RLG00000033815
rosa_multiflora Rmu_sc0005416.1_g000014
rosa_roxburghii Rroxscaffold_1G00043090
rosa_rugosa Rorug05G0168000
rosa_samantha Rh5AG256800 Rh5BG259200 Rh5CG292200 Rh5DG269100
rosa_wichuraiana Rw5G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 596
AccII CGCG 1 cut(s) 231
AciI CCGC 1 cut(s) 540
AclWI GGATC 2 cut(s) 567, 580
AcoI YGGCCR 4 cut(s) 3, 211, 235, 295
AfiI CCNNNNNNNGG 3 cut(s) 56, 476, 539
AgsI TTSAA 4 cut(s) 176, 199, 316, 552
AjnI CCWGG 3 cut(s) 208, 237, 475
AluBI AGCT 2 cut(s) 116, 278
AluI AGCT 2 cut(s) 116, 278
Alw26I GTCTC 2 cut(s) 155, 368
AlwI GGATC 2 cut(s) 567, 580
AlwNI CAGNNNCTG 1 cut(s) 590
AoxI GGCC 5 cut(s) 3, 211, 235, 295, 326
ApeKI GCWGC 1 cut(s) 590
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 194, 465
BalI TGGCCA 3 cut(s) 5, 213, 297
BamHI GGATCC 1 cut(s) 572
BarI GAAGNNNNNNTAC 2 cut(s) 45, 77
BbsI GAAGAC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 577
BccI CCATC 4 cut(s) 287, 373, 439, 511
BciT130I CCWGG 3 cut(s) 210, 239, 477
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 2 cut(s) 155, 368
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 3 cut(s) 210, 239, 477
BmgT120I GGNCC 1 cut(s) 326
BmiI GGNNCC 2 cut(s) 407, 574
BmrFI CCNGG 3 cut(s) 210, 239, 477
BmsI GCATC 3 cut(s) 121, 143, 256
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 470
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 476
BsaXI ACNNNNNCTCC 6 cut(s) 253, 283, 418, 448, 490, 520
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 476, 539
Bse3DI GCAATG 2 cut(s) 39, 424
BseBI CCWGG 3 cut(s) 210, 239, 477
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 3 cut(s) 56, 476, 539
BseMI GCAATG 2 cut(s) 39, 424
BseMII CTCAG 2 cut(s) 234, 270
BseRI GAGGAG 2 cut(s) 519, 522
BseXI GCAGC 1 cut(s) 577
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 5 cut(s) 5, 213, 237, 297, 328
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 3 cut(s) 56, 476, 539
BsmAI GTCTC 2 cut(s) 155, 368
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 5 cut(s) 5, 213, 237, 297, 328
Bso31I GGTCTC 1 cut(s) 155
Bsp143I GATC 3 cut(s) 489, 517, 572
BspACI CCGC 1 cut(s) 540
BspANI GGCC 5 cut(s) 5, 213, 237, 297, 328
BspCNI CTCAG 2 cut(s) 233, 271
BspFNI CGCG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 407, 574
BspMAI CTGCAG 1 cut(s) 87
BspPI GGATC 2 cut(s) 567, 580
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 2 cut(s) 39, 424
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 3 cut(s) 489, 517, 572
BssNAI GTATAC 1 cut(s) 597
Bst1107I GTATAC 1 cut(s) 597
Bst2UI CCWGG 3 cut(s) 210, 239, 477
Bst4CI ACNGT 3 cut(s) 112, 124, 362
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 590
BstDEI CTNAG 3 cut(s) 20, 220, 279
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 231
BstKTI GATC 3 cut(s) 492, 520, 575
BstMAI GTCTC 2 cut(s) 155, 368
BstMBI GATC 3 cut(s) 489, 517, 572
BstMWI GCNNNNNNNGC 3 cut(s) 82, 275, 590
BstNI CCWGG 3 cut(s) 210, 239, 477
BstSCI CCNGG 3 cut(s) 208, 237, 475
BstSFI CTRYAG 1 cut(s) 83
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 577
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 572
BstYI RGATCY 1 cut(s) 572
BstZ17I GTATAC 1 cut(s) 597
BsuRI GGCC 5 cut(s) 5, 213, 237, 297, 328
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 228
CaiI CAGNNNCTG 1 cut(s) 590
CciI TCATGA 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 326
CseI GACGC 2 cut(s) 220, 490
CviAII CATG 1 cut(s) 340
CviJI RGCY 8 cut(s) 5, 116, 213, 237, 278, 297, 328, 455
CviKI_1 RGCY 8 cut(s) 5, 116, 213, 237, 278, 297, 328, 455
DdeI CTNAG 3 cut(s) 20, 220, 279
DpnI GATC 3 cut(s) 491, 519, 574
DpnII GATC 3 cut(s) 489, 517, 572
EaeI YGGCCR 4 cut(s) 3, 211, 235, 295
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 155
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoRII CCWGG 3 cut(s) 208, 237, 475
EcoT22I ATGCAT 1 cut(s) 136
FaeI CATG 1 cut(s) 343
FaiI YATR 7 cut(s) 28, 30, 147, 191, 288, 341, 597
FaqI GGGAC 1 cut(s) 493
FatI CATG 1 cut(s) 339
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 1 cut(s) 537
GluI GCNGC 1 cut(s) 591
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 5 cut(s) 5, 213, 237, 297, 328
HgaI GACGC 2 cut(s) 220, 490
Hin1II CATG 1 cut(s) 343
HinfI GANTC 2 cut(s) 185, 218
HphI GGTGA 2 cut(s) 194, 465
Hpy166II GTNNAC 2 cut(s) 108, 597
Hpy188III TCNNGA 4 cut(s) 128, 340, 487, 509
Hpy8I GTNNAC 2 cut(s) 108, 597
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 2 cut(s) 453, 480
HpyCH4III ACNGT 3 cut(s) 112, 124, 362
HpyCH4V TGCA 7 cut(s) 44, 85, 98, 134, 258, 422, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 275, 590
HpyF3I CTNAG 3 cut(s) 20, 220, 279
Hsp92II CATG 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 3 cut(s) 489, 517, 572
LmnI GCTCC 3 cut(s) 274, 489, 581
Lsp1109I GCAGC 1 cut(s) 577
LweI GCATC 3 cut(s) 121, 143, 256
MaeI CTAG 1 cut(s) 537
MaeIII GTNAC 2 cut(s) 78, 182
MalI GATC 3 cut(s) 491, 519, 574
MboI GATC 3 cut(s) 489, 517, 572
MboII GAAGA 4 cut(s) 53, 100, 388, 540
MflI RGATCY 1 cut(s) 572
MlsI TGGCCA 3 cut(s) 5, 213, 297
MluCI AATT 3 cut(s) 177, 253, 552
MluNI TGGCCA 3 cut(s) 5, 213, 297
MlyI GAGTC 2 cut(s) 179, 212
MmeI TCCRAC 2 cut(s) 296, 550
MnlI CCTC 5 cut(s) 466, 474, 537, 540, 543
Mox20I TGGCCA 3 cut(s) 5, 213, 297
Mph1103I ATGCAT 1 cut(s) 136
MscI TGGCCA 3 cut(s) 5, 213, 297
Msp20I TGGCCA 3 cut(s) 5, 213, 297
MspA1I CMGCKG 1 cut(s) 278
MspR9I CCNGG 3 cut(s) 210, 239, 477
MvaI CCWGG 3 cut(s) 210, 239, 477
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 3 cut(s) 82, 275, 590
NdeII GATC 3 cut(s) 489, 517, 572
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 2 cut(s) 407, 574
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 136
PagI TCATGA 1 cut(s) 339
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 2 cut(s) 179, 212
PpsI GAGTC 2 cut(s) 179, 212
Psp6I CCWGG 3 cut(s) 208, 237, 475
PspGI CCWGG 3 cut(s) 208, 237, 475
PspN4I GGNNCC 2 cut(s) 407, 574
PspPI GGNCC 1 cut(s) 326
PstI CTGCAG 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 1 cut(s) 572
PvuII CAGCTG 1 cut(s) 278
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 3 cut(s) 489, 517, 572
Sau96I GGNCC 1 cut(s) 326
SchI GAGTC 2 cut(s) 179, 212
ScrFI CCNGG 3 cut(s) 210, 239, 477
SetI ASST 5 cut(s) 118, 280, 308, 445, 466
SfaNI GCATC 3 cut(s) 121, 143, 256
SfcI CTRYAG 1 cut(s) 83
Sse9I AATT 3 cut(s) 177, 253, 552
SsiI CCGC 1 cut(s) 540
SspI AATATT 1 cut(s) 16
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 3 cut(s) 208, 237, 475
TaaI ACNGT 3 cut(s) 112, 124, 362
TaqII GACCGA 1 cut(s) 47
TasI AATT 3 cut(s) 177, 253, 552
TscAI CASTG 1 cut(s) 228
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 590
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 328, 547
TspRI CASTG 1 cut(s) 228
XagI CCTNNNNNAGG 1 cut(s) 474
XmiI GTMKAC 1 cut(s) 596
XspI CTAG 1 cut(s) 537
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.