Rw5G023810

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014590
Physical Location & Seq
Forward (+)
33664817 .. 33666229
1413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023810.1

Sequence Viewer

Length: 621 bp
ATGGCCAACTCTAATATTCTAAGAGCATATAACAAAAGCATTGCATTTCCTGTATTGAGTCTTCTTTGTCTATCGCTGTTACTGCAGAAGAGTGGTGCAAATGAGTTTACAATAGCTTGGACTGTTCCTGATGCATCAACTCATTTTAGTCAATGGGCAGAGACCAACAGATTTCAAATTGGTGACTCCTTAGTGTTCAACTATTCTCCTGGCCAAGACTCAGTGCTTCGTGTCGGCCAGGATGACTACACAAATTGCAATACTGATGCTCCAACAGCTGAGAAATATACCGATGGCCACTCTACCTACAAGTTCAACCAATCTGGGCCATTCTACTTCATGAGTGGGAACAAAGATAACTGTCTCAAAAATGAGAAGATGGTTGTGATTGTCTTGGCAGACAGGAACCAAACAAGCACTGCAACTCCTCCATCTCCTGCACCTTCTGGCGAAGCGTCTCCACCTCCCCCTTCACCAGGGACGGTCCAGATTAACCCGACTCCAGCTCCCGAAAGTGATCAAATGCCGCCTCCACCTCCTAGCGGTGCTTCTTCAATTTTCATCAGTTTAGTTGGAACCATTGGAGCAGTTGCTGCGTATTCATCATTTTTATTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

206

Amino Acids

22.06

Weight (kDa)

4.85

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 123 5e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20570
fragaria_vesca FvH4_3g21980
malus_domestica MD03G1209600.v1.1 MD11G1224400.v1.1
prunus_persica Prupe.4G205500_v2.0.a1
pyrus_communis pycom03g15840 pycom11g19810
rosa_chinensis RchiOBHm_Chr5g0037831
rosa_laevigata RLG00000033815
rosa_multiflora Rmu_sc0005416.1_g000014
rosa_roxburghii Rroxscaffold_1G00043090
rosa_rugosa Rorug05G0168000
rosa_samantha Rh5AG256800 Rh5BG259200 Rh5CG292200 Rh5DG269100
rosa_wichuraiana Rw5G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 527, 543
AcoI YGGCCR 4 cut(s) 3, 211, 235, 295
AfiI CCNNNNNNNGG 2 cut(s) 476, 542
AgsI TTSAA 4 cut(s) 176, 199, 316, 555
AjnI CCWGG 3 cut(s) 208, 237, 475
AluBI AGCT 3 cut(s) 116, 278, 506
AluI AGCT 3 cut(s) 116, 278, 506
Alw26I GTCTC 3 cut(s) 155, 368, 462
AlwNI CAGNNNCTG 1 cut(s) 593
AoxI GGCC 5 cut(s) 3, 211, 235, 295, 326
ApeKI GCWGC 1 cut(s) 593
AspS9I GGNCC 2 cut(s) 326, 484
AsuHPI GGTGA 2 cut(s) 194, 465
AvaII GGWCC 1 cut(s) 484
BalI TGGCCA 3 cut(s) 5, 213, 297
BarI GAAGNNNNNNTAC 2 cut(s) 45, 77
BbsI GAAGAC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 580
BccI CCATC 3 cut(s) 287, 373, 439
BciT130I CCWGG 3 cut(s) 210, 239, 477
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 3 cut(s) 155, 368, 462
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 527, 594
BlsI GCNGC 2 cut(s) 528, 595
Bme1390I CCNGG 3 cut(s) 210, 239, 477
Bme18I GGWCC 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 326, 484
BmiI GGNNCC 2 cut(s) 407, 577
BmrFI CCNGG 3 cut(s) 210, 239, 477
BmsI GCATC 3 cut(s) 121, 143, 256
BpiI GAAGAC 1 cut(s) 53
BpmI CTGGAG 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 476
BsaXI ACNNNNNCTCC 6 cut(s) 253, 283, 409, 439, 490, 520
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 542
Bse3DI GCAATG 1 cut(s) 39
BseBI CCWGG 3 cut(s) 210, 239, 477
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 476, 542
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 234, 270
BseRI GAGGAG 1 cut(s) 417
BseXI GCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 423
BshFI GGCC 5 cut(s) 5, 213, 237, 297, 328
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 2 cut(s) 476, 542
BsmAI GTCTC 3 cut(s) 155, 368, 462
BsmBI CGTCTC 1 cut(s) 462
BsmFI GGGAC 1 cut(s) 493
BsnI GGCC 5 cut(s) 5, 213, 237, 297, 328
Bso31I GGTCTC 1 cut(s) 155
Bsp143I GATC 1 cut(s) 517
BspACI CCGC 2 cut(s) 527, 543
BspANI GGCC 5 cut(s) 5, 213, 237, 297, 328
BspCNI CTCAG 2 cut(s) 233, 271
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 407, 577
BspMAI CTGCAG 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 1 cut(s) 517
Bst2UI CCWGG 3 cut(s) 210, 239, 477
Bst4CI ACNGT 3 cut(s) 124, 362, 484
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 593
BstDEI CTNAG 4 cut(s) 20, 190, 220, 279
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 3 cut(s) 155, 368, 462
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 3 cut(s) 82, 275, 593
BstNI CCWGG 3 cut(s) 210, 239, 477
BstSCI CCNGG 3 cut(s) 208, 237, 475
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 1 cut(s) 53
BsuRI GGCC 5 cut(s) 5, 213, 237, 297, 328
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 228, 417
CaiI CAGNNNCTG 1 cut(s) 593
CciI TCATGA 1 cut(s) 339
Cfr13I GGNCC 2 cut(s) 326, 484
CseI GACGC 1 cut(s) 444
CviAII CATG 1 cut(s) 340
CviJI RGCY 8 cut(s) 5, 116, 213, 237, 278, 297, 328, 506
CviKI_1 RGCY 8 cut(s) 5, 116, 213, 237, 278, 297, 328, 506
DdeI CTNAG 4 cut(s) 20, 190, 220, 279
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
EaeI YGGCCR 4 cut(s) 3, 211, 235, 295
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 484
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoRII CCWGG 3 cut(s) 208, 237, 475
EcoT22I ATGCAT 1 cut(s) 136
Esp3I CGTCTC 1 cut(s) 462
FaeI CATG 1 cut(s) 343
FaiI YATR 4 cut(s) 28, 30, 288, 341
FaqI GGGAC 1 cut(s) 493
FatI CATG 1 cut(s) 339
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 2 cut(s) 527, 594
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 2 cut(s) 527, 594
FspBI CTAG 1 cut(s) 540
GluI GCNGC 2 cut(s) 527, 594
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 5 cut(s) 5, 213, 237, 297, 328
HgaI GACGC 1 cut(s) 444
Hin1II CATG 1 cut(s) 343
HinfI GANTC 4 cut(s) 58, 185, 218, 499
HphI GGTGA 2 cut(s) 194, 465
Hpy166II GTNNAC 1 cut(s) 108
Hpy188III TCNNGA 4 cut(s) 128, 340, 487, 509
Hpy8I GTNNAC 1 cut(s) 108
HpyAV CCTTC 2 cut(s) 453, 480
HpyCH4III ACNGT 3 cut(s) 124, 362, 484
HpyCH4V TGCA 7 cut(s) 44, 85, 98, 134, 258, 422, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 275, 593
HpyF3I CTNAG 4 cut(s) 20, 190, 220, 279
Hsp92II CATG 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 3 cut(s) 274, 511, 584
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 3 cut(s) 121, 143, 256
MaeI CTAG 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 78, 182
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 4 cut(s) 53, 100, 388, 543
MlsI TGGCCA 3 cut(s) 5, 213, 297
MluCI AATT 3 cut(s) 177, 253, 555
MluNI TGGCCA 3 cut(s) 5, 213, 297
MlyI GAGTC 4 cut(s) 67, 179, 212, 493
MmeI TCCRAC 2 cut(s) 296, 553
MnlI CCTC 4 cut(s) 438, 474, 540, 546
Mox20I TGGCCA 3 cut(s) 5, 213, 297
Mph1103I ATGCAT 1 cut(s) 136
MscI TGGCCA 3 cut(s) 5, 213, 297
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 3 cut(s) 5, 213, 297
MspA1I CMGCKG 1 cut(s) 278
MspR9I CCNGG 3 cut(s) 210, 239, 477
MvaI CCWGG 3 cut(s) 210, 239, 477
MwoI GCNNNNNNNGC 3 cut(s) 82, 275, 593
NdeII GATC 1 cut(s) 517
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 2 cut(s) 407, 577
NmuCI GTSAC 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 136
PagI TCATGA 1 cut(s) 339
PkrI GCNGC 2 cut(s) 528, 595
PleI GAGTC 4 cut(s) 66, 179, 212, 493
PpsI GAGTC 4 cut(s) 66, 179, 212, 493
Psp6I CCWGG 3 cut(s) 208, 237, 475
PspGI CCWGG 3 cut(s) 208, 237, 475
PspN4I GGNNCC 2 cut(s) 407, 577
PspPI GGNCC 2 cut(s) 326, 484
PstI CTGCAG 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 593
PvuII CAGCTG 1 cut(s) 278
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 2 cut(s) 527, 594
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 2 cut(s) 326, 484
SchI GAGTC 4 cut(s) 67, 179, 212, 493
ScrFI CCNGG 3 cut(s) 210, 239, 477
SetI ASST 7 cut(s) 118, 280, 308, 445, 466, 508, 538
SfaNI GCATC 3 cut(s) 121, 143, 256
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 484
Sse9I AATT 3 cut(s) 177, 253, 555
SsiI CCGC 2 cut(s) 527, 543
SspI AATATT 1 cut(s) 16
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 3 cut(s) 208, 237, 475
TaaI ACNGT 3 cut(s) 124, 362, 484
TasI AATT 3 cut(s) 177, 253, 555
TauI GCSGC 1 cut(s) 529
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 2 cut(s) 228, 424
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 3 cut(s) 328, 550, 591
TspRI CASTG 2 cut(s) 228, 424
VpaK11BI GGWCC 1 cut(s) 484
XagI CCTNNNNNAGG 1 cut(s) 474
XspI CTAG 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.