MD11G1224400.v1.1

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014590
Physical Location & Seq
Reverse (-)
32846766 .. 32848526
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1224400.v1.1.491

Sequence Viewer

Length: 645 bp
ATGGCCAACCAAATTCACAAAAATAATATTAATGCAGTTTTTGTTCTGGGGATTGTATGCGTGACGCTGTTGATACAGAAGAGTGGTGCAACTGACTTTGCAATTGGAGGCTCAAAGGGCTGGTGTATTCCTGATTCTTCTGTTCACTATAATCAATGGGCAGAAAGAAACAGGTTTCAAATCGGAGACTCCATTGTGTTCCACTACCCTCCTGGCCAAGACTCAGTGCTTCTAGTAACCCAGGACGACTACACCAACTGCAACACGGATTCACCTACGGCTAAGAAATACACCGATGGCCACACTGTCTTCAAGTTCAACCAATCCGGGCCATTCTACTTCATCAGTGGGAACAAAGATAAATGTCAGAAAAATGAGAAGGTGGTAGTGATTGTCTTGGCAGAGAGAAGCAGAAATGCAAAACCCCCTTCATCTTCAAACTCAAAAGATATGATCCCATCTCCAGCTCCTTCTGATGAAGCGTCTCCACCTCCACCGCCGTCAGGGACTACTGAGATTAACCCTACTCCTGCTCCTGAAGGTGAAACACCTCCTCCACCTCCGAACGCGGCTTCTTCAGTTTTCATGAGTTTTATTGGGTCCGTCGGAGCATTTGCTGCATCCTCAACACTTTTACTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

215

Amino Acids

22.91

Weight (kDa)

6.05

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 41 - 124 2.5e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20570
fragaria_vesca FvH4_3g21980
malus_domestica MD03G1209600.v1.1 MD11G1224400.v1.1
prunus_persica Prupe.4G205500_v2.0.a1
pyrus_communis pycom03g15840 pycom11g19810
rosa_chinensis RchiOBHm_Chr5g0037831
rosa_laevigata RLG00000033815
rosa_multiflora Rmu_sc0005416.1_g000014
rosa_roxburghii Rroxscaffold_1G00043090
rosa_rugosa Rorug05G0168000
rosa_samantha Rh5AG256800 Rh5BG259200 Rh5CG292200 Rh5DG269100
rosa_wichuraiana Rw5G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 569
AciI CCGC 2 cut(s) 497, 569
AclWI GGATC 1 cut(s) 448
AcoI YGGCCR 3 cut(s) 3, 214, 298
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 2 cut(s) 558, 561
AfiI CCNNNNNNNGG 1 cut(s) 503
AgsI TTSAA 4 cut(s) 179, 313, 319, 438
AjnI CCWGG 2 cut(s) 211, 240
AluBI AGCT 1 cut(s) 467
AluI AGCT 1 cut(s) 467
Alw26I GTCTC 2 cut(s) 180, 489
AlwI GGATC 1 cut(s) 448
AoxI GGCC 4 cut(s) 3, 214, 298, 329
ApeKI GCWGC 1 cut(s) 617
ApoI RAATTY 1 cut(s) 12
AseI ATTAAT 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 329, 600
AsuC2I CCSGG 1 cut(s) 328
AsuHPI GGTGA 2 cut(s) 264, 554
AvaII GGWCC 1 cut(s) 600
BalI TGGCCA 3 cut(s) 5, 216, 300
BbsI GAAGAC 1 cut(s) 301
BbvI GCAGC 1 cut(s) 604
BccI CCATC 2 cut(s) 290, 466
BceAI ACGGC 2 cut(s) 294, 484
BciT130I CCWGG 2 cut(s) 213, 242
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 180, 489
BfaI CTAG 1 cut(s) 233
BisI GCNGC 2 cut(s) 570, 618
BlsI GCNGC 2 cut(s) 571, 619
Bme1390I CCNGG 3 cut(s) 213, 242, 328
Bme18I GGWCC 1 cut(s) 600
BmgT120I GGNCC 2 cut(s) 329, 600
BmiI GGNNCC 1 cut(s) 601
BmrFI CCNGG 3 cut(s) 213, 242, 328
BmsI GCATC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 301
BpmI CTGGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 517, 538, 547, 568
Bsc4I CCNNNNNNNGG 1 cut(s) 503
BseBI CCWGG 2 cut(s) 213, 242
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 620
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMII CTCAG 2 cut(s) 237, 504
BseRI GAGGAG 1 cut(s) 543
BseXI GCAGC 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 569
BshFI GGCC 4 cut(s) 5, 216, 300, 331
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 520
BslI CCNNNNNNNGG 1 cut(s) 503
BsmAI GTCTC 2 cut(s) 180, 489
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 1 cut(s) 520
BsnI GGCC 4 cut(s) 5, 216, 300, 331
Bsp143I GATC 1 cut(s) 453
BspACI CCGC 2 cut(s) 497, 569
BspANI GGCC 4 cut(s) 5, 216, 300, 331
BspCNI CTCAG 2 cut(s) 236, 505
BspFNI CGCG 1 cut(s) 569
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 1 cut(s) 601
BspPI GGATC 1 cut(s) 448
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 213, 242
Bst4CI ACNGT 1 cut(s) 307
Bst6I CTCTTC 1 cut(s) 74
BstAPI GCANNNNNTGC 1 cut(s) 617
BstDEI CTNAG 3 cut(s) 223, 282, 513
BstF5I GGATG 1 cut(s) 620
BstFNI CGCG 1 cut(s) 569
BstKTI GATC 1 cut(s) 456
BstMAI GTCTC 2 cut(s) 180, 489
BstMBI GATC 1 cut(s) 453
BstMWI GCNNNNNNNGC 2 cut(s) 117, 617
BstNI CCWGG 2 cut(s) 213, 242
BstSCI CCNGG 3 cut(s) 211, 240, 326
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 604
BstV2I GAAGAC 1 cut(s) 301
BsuRI GGCC 4 cut(s) 5, 216, 300, 331
BtsCI GGATG 1 cut(s) 620
BtsIMutI CAGTG 3 cut(s) 231, 303, 352
CciI TCATGA 1 cut(s) 585
Cfr13I GGNCC 2 cut(s) 329, 600
CseI GACGC 2 cut(s) 73, 471
CviAII CATG 1 cut(s) 586
CviJI RGCY 9 cut(s) 5, 111, 120, 216, 281, 300, 331, 467, 572
CviKI_1 RGCY 9 cut(s) 5, 111, 120, 216, 281, 300, 331, 467, 572
DdeI CTNAG 3 cut(s) 223, 282, 513
DpnI GATC 1 cut(s) 455
DpnII GATC 1 cut(s) 453
EaeI YGGCCR 3 cut(s) 3, 214, 298
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 600
Eco57I CTGAAG 2 cut(s) 558, 561
EcoRII CCWGG 2 cut(s) 211, 240
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 1 cut(s) 589
FaiI YATR 4 cut(s) 58, 150, 452, 587
FaqI GGGAC 1 cut(s) 520
FatI CATG 1 cut(s) 585
Fnu4HI GCNGC 2 cut(s) 570, 618
FokI GGATG 1 cut(s) 607
Fsp4HI GCNGC 2 cut(s) 570, 618
FspBI CTAG 1 cut(s) 233
GluI GCNGC 2 cut(s) 570, 618
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 4 cut(s) 5, 216, 300, 331
HapII CCGG 1 cut(s) 327
HgaI GACGC 2 cut(s) 73, 471
Hin1II CATG 1 cut(s) 589
HinfI GANTC 4 cut(s) 134, 188, 221, 269
HpaII CCGG 1 cut(s) 327
HphI GGTGA 2 cut(s) 264, 554
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 5 cut(s) 185, 369, 475, 564, 608
Hpy188III TCNNGA 3 cut(s) 131, 536, 586
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 4 cut(s) 373, 438, 480, 533
HpyCH4III ACNGT 1 cut(s) 307
HpyCH4V TGCA 6 cut(s) 35, 89, 101, 261, 419, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 617
HpyF3I CTNAG 3 cut(s) 223, 282, 513
Hsp92II CATG 1 cut(s) 589
Kzo9I GATC 1 cut(s) 453
LmnI GCTCC 3 cut(s) 472, 538, 608
Lsp1109I GCAGC 1 cut(s) 604
LweI GCATC 1 cut(s) 629
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 61, 235
MalI GATC 1 cut(s) 455
MboI GATC 1 cut(s) 453
MboII GAAGA 5 cut(s) 91, 129, 301, 426, 567
MfeI CAATTG 1 cut(s) 102
MlsI TGGCCA 3 cut(s) 5, 216, 300
MluCI AATT 2 cut(s) 12, 102
MluNI TGGCCA 3 cut(s) 5, 216, 300
MlyI GAGTC 2 cut(s) 182, 215
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 7 cut(s) 101, 219, 501, 561, 564, 570, 634
Mox20I TGGCCA 3 cut(s) 5, 216, 300
MscI TGGCCA 3 cut(s) 5, 216, 300
MseI TTAA 2 cut(s) 30, 519
Msp20I TGGCCA 3 cut(s) 5, 216, 300
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 3 cut(s) 213, 242, 328
MunI CAATTG 1 cut(s) 102
MvaI CCWGG 2 cut(s) 213, 242
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 2 cut(s) 117, 617
NciI CCSGG 1 cut(s) 328
NdeII GATC 1 cut(s) 453
NlaIII CATG 1 cut(s) 589
NlaIV GGNNCC 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 61
PagI TCATGA 1 cut(s) 585
PfeI GAWTC 2 cut(s) 134, 269
PkrI GCNGC 2 cut(s) 571, 619
PleI GAGTC 2 cut(s) 182, 215
PpsI GAGTC 2 cut(s) 182, 215
PshBI ATTAAT 1 cut(s) 30
Psp6I CCWGG 2 cut(s) 211, 240
PspGI CCWGG 2 cut(s) 211, 240
PspN4I GGNNCC 1 cut(s) 601
PspPI GGNCC 2 cut(s) 329, 600
SaqAI TTAA 2 cut(s) 30, 519
SatI GCNGC 2 cut(s) 570, 618
Sau3AI GATC 1 cut(s) 453
Sau96I GGNCC 2 cut(s) 329, 600
SchI GAGTC 2 cut(s) 182, 215
ScrFI CCNGG 3 cut(s) 213, 242, 328
SetI ASST 8 cut(s) 176, 277, 384, 469, 493, 544, 553, 562
SfaNI GCATC 1 cut(s) 629
SinI GGWCC 1 cut(s) 600
Sse9I AATT 2 cut(s) 12, 102
SsiI CCGC 2 cut(s) 497, 569
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 233
StyD4I CCNGG 3 cut(s) 211, 240, 326
TaaI ACNGT 1 cut(s) 307
TasI AATT 2 cut(s) 12, 102
TauI GCSGC 1 cut(s) 572
TfiI GAWTC 2 cut(s) 134, 269
Tru1I TTAA 2 cut(s) 30, 519
Tru9I TTAA 2 cut(s) 30, 519
TscAI CASTG 3 cut(s) 231, 310, 352
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 617
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 331, 420, 492, 574
TspGWI ACGGA 2 cut(s) 281, 592
TspRI CASTG 3 cut(s) 231, 310, 352
VpaK11BI GGWCC 1 cut(s) 600
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 1 cut(s) 12
XcmI CCANNNNNNNNNTGG 1 cut(s) 209
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.