pycom03g15840

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014590
Physical Location & Seq
Reverse (-)
17087727 .. 17088863
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15840.2

Sequence Viewer

Length: 612 bp
ATGGCCAACTCCATTCACAAAAATAATATTAATGCAGTTTTTGTTCTGGGGATCGTATGTGTGATGCTGTTGATACAGAAGAGTGGTGCAACTGACTTTGCAATTGGAGGCTCAAGGGGTTGGGCTGTTCCAGATTCTTCTACTCACTATAGTCAATGGGCAGAAGGAAACAGGTTTCAAATCGGAGACTCCATTGTGTTCAACTACTCTCCTGGACAAGACTCAGTGCTTCGAGTAACCCAGGATGACTACAACAACTGCAACACTGATTCGCCTTCGGCCGAGAAATACTCTGATGGCCACACTGCCTTCCAGTTTAACCAATCTGGACCGTTCTATTTCATCAGTGGGAACAAAGACAACTGTCTCAAAAATGAGAAGGTGCTAGTGATTGTCTTGGCAGACAGAAGCAACAGAAATGCAACTCCTCCTTCACCTTCAAACTCAACAGATATGACCCCATCTCCAGCTCCCTCTGGTGAGGCGTCTCCACCTCCAACTCCAGCTCCTGAAACACCTCCTCCACCTCCGAATGCGGCTTCTTCAATTTTCATGAGTTTTGTTGGGTCCGTCGGAGCATTTGCTGCATCCTCAACACTTTTACTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

204

Amino Acids

21.52

Weight (kDa)

4.99

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20570
fragaria_vesca FvH4_3g21980
malus_domestica MD03G1209600.v1.1 MD11G1224400.v1.1
prunus_persica Prupe.4G205500_v2.0.a1
pyrus_communis pycom03g15840 pycom11g19810
rosa_chinensis RchiOBHm_Chr5g0037831
rosa_laevigata RLG00000033815
rosa_multiflora Rmu_sc0005416.1_g000014
rosa_roxburghii Rroxscaffold_1G00043090
rosa_rugosa Rorug05G0168000
rosa_samantha Rh5AG256800 Rh5BG259200 Rh5CG292200 Rh5DG269100
rosa_wichuraiana Rw5G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 536
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 3 cut(s) 3, 279, 298
AcyI GRCGYC 1 cut(s) 485
AgsI TTSAA 4 cut(s) 179, 202, 441, 546
AjnI CCWGG 2 cut(s) 211, 240
AluBI AGCT 2 cut(s) 470, 506
AluI AGCT 2 cut(s) 470, 506
Alw26I GTCTC 3 cut(s) 180, 371, 492
AlwI GGATC 1 cut(s) 59
AlwNI CAGNNNCTG 1 cut(s) 509
AoxI GGCC 3 cut(s) 3, 279, 298
ApeKI GCWGC 1 cut(s) 584
AseI ATTAAT 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 329, 567
AsuHPI GGTGA 2 cut(s) 426, 491
AvaII GGWCC 2 cut(s) 329, 567
BalI TGGCCA 2 cut(s) 5, 300
BbvI GCAGC 1 cut(s) 571
BccI CCATC 2 cut(s) 290, 469
BciT130I CCWGG 2 cut(s) 213, 242
BcoDI GTCTC 3 cut(s) 180, 371, 492
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 1 cut(s) 148
BisI GCNGC 2 cut(s) 537, 585
BlsI GCNGC 2 cut(s) 538, 586
Bme1390I CCNGG 2 cut(s) 213, 242
Bme18I GGWCC 2 cut(s) 329, 567
BmgT120I GGNCC 2 cut(s) 329, 567
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 2 cut(s) 213, 242
BmsI GCATC 2 cut(s) 54, 596
BoxI GACNNNNGTC 1 cut(s) 363
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 2 cut(s) 450, 486
BpuEI CTTGAG 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 6 cut(s) 448, 478, 490, 505, 520, 535
Bse1I ACTGG 1 cut(s) 313
BseBI CCWGG 2 cut(s) 213, 242
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 250, 587
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 417, 510
BseX3I CGGCCG 1 cut(s) 279
BseXI GCAGC 1 cut(s) 571
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 3 cut(s) 5, 281, 300
BsiEI CGRYCG 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 180, 371, 492
BsmBI CGTCTC 1 cut(s) 492
BsmI GAATGC 1 cut(s) 538
BsnI GGCC 3 cut(s) 5, 281, 300
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 1 cut(s) 536
BspANI GGCC 3 cut(s) 5, 281, 300
BspCNI CTCAG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 552
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 1 cut(s) 59
BsrI ACTGG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 51
BssNI GRCGYC 1 cut(s) 485
Bst2UI CCWGG 2 cut(s) 213, 242
Bst4CI ACNGT 2 cut(s) 333, 365
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 1 cut(s) 485
BstAPI GCANNNNNTGC 1 cut(s) 584
BstDEI CTNAG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 250, 587
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 3 cut(s) 180, 371, 492
BstMBI GATC 1 cut(s) 51
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 584
BstNI CCWGG 2 cut(s) 213, 242
BstPAI GACNNNNGTC 1 cut(s) 363
BstSCI CCNGG 2 cut(s) 211, 240
BstSFI CTRYAG 1 cut(s) 148
BstV1I GCAGC 1 cut(s) 571
BstZI CGGCCG 1 cut(s) 279
BsuRI GGCC 3 cut(s) 5, 281, 300
BtsCI GGATG 2 cut(s) 250, 587
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 4 cut(s) 231, 264, 303, 352
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 552
Cfr13I GGNCC 2 cut(s) 329, 567
CseI GACGC 1 cut(s) 474
CviAII CATG 1 cut(s) 553
CviJI RGCY 8 cut(s) 5, 111, 125, 281, 300, 470, 506, 539
CviKI_1 RGCY 8 cut(s) 5, 111, 125, 281, 300, 470, 506, 539
DdeI CTNAG 1 cut(s) 223
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 3 cut(s) 3, 279, 298
EagI CGGCCG 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 279
Eco47I GGWCC 2 cut(s) 329, 567
Eco52I CGGCCG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 211, 240
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 1 cut(s) 556
FaiI YATR 4 cut(s) 58, 150, 455, 554
FatI CATG 1 cut(s) 552
Fnu4HI GCNGC 2 cut(s) 537, 585
FokI GGATG 2 cut(s) 257, 574
Fsp4HI GCNGC 2 cut(s) 537, 585
FspBI CTAG 1 cut(s) 386
GluI GCNGC 2 cut(s) 537, 585
GsuI CTGGAG 2 cut(s) 450, 486
HaeIII GGCC 3 cut(s) 5, 281, 300
HgaI GACGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 485
Hin1II CATG 1 cut(s) 556
HinfI GANTC 4 cut(s) 134, 188, 221, 269
HphI GGTGA 2 cut(s) 426, 491
Hpy188I TCNGA 4 cut(s) 185, 295, 531, 575
Hpy188III TCNNGA 4 cut(s) 131, 327, 509, 553
Hpy99I CGWCG 1 cut(s) 575
HpyAV CCTTC 6 cut(s) 158, 285, 319, 373, 441, 447
HpyCH4III ACNGT 2 cut(s) 333, 365
HpyCH4V TGCA 6 cut(s) 35, 89, 101, 261, 422, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 584
HpyF3I CTNAG 1 cut(s) 223
Hsp92I GRCGYC 1 cut(s) 485
Hsp92II CATG 1 cut(s) 556
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 3 cut(s) 475, 511, 575
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 2 cut(s) 54, 596
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 3 cut(s) 91, 129, 534
MfeI CAATTG 1 cut(s) 102
MlsI TGGCCA 2 cut(s) 5, 300
MluCI AATT 2 cut(s) 102, 546
MluNI TGGCCA 2 cut(s) 5, 300
MlyI GAGTC 2 cut(s) 182, 215
MmeI TCCRAC 2 cut(s) 521, 553
MnlI CCTC 9 cut(s) 101, 438, 475, 484, 504, 528, 531, 537, 601
Mox20I TGGCCA 2 cut(s) 5, 300
MscI TGGCCA 2 cut(s) 5, 300
MseI TTAA 2 cut(s) 30, 318
Msp20I TGGCCA 2 cut(s) 5, 300
MspR9I CCNGG 2 cut(s) 213, 242
MunI CAATTG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 538
MvaI CCWGG 2 cut(s) 213, 242
MwoI GCNNNNNNNGC 1 cut(s) 584
NdeII GATC 1 cut(s) 51
NlaIII CATG 1 cut(s) 556
NlaIV GGNNCC 1 cut(s) 568
NmeAIII GCCGAG 1 cut(s) 307
PagI TCATGA 1 cut(s) 552
PctI GAATGC 1 cut(s) 538
PfeI GAWTC 2 cut(s) 134, 269
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 2 cut(s) 538, 586
PleI GAGTC 2 cut(s) 182, 215
PpsI GAGTC 2 cut(s) 182, 215
PshAI GACNNNNGTC 1 cut(s) 363
PshBI ATTAAT 1 cut(s) 30
Psp6I CCWGG 2 cut(s) 211, 240
PspGI CCWGG 2 cut(s) 211, 240
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 2 cut(s) 329, 567
PstNI CAGNNNCTG 1 cut(s) 509
SaqAI TTAA 2 cut(s) 30, 318
SatI GCNGC 2 cut(s) 537, 585
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 2 cut(s) 329, 567
SchI GAGTC 2 cut(s) 182, 215
ScrFI CCNGG 2 cut(s) 213, 242
SetI ASST 8 cut(s) 176, 384, 439, 472, 496, 508, 520, 529
SfaNI GCATC 2 cut(s) 54, 596
SfcI CTRYAG 1 cut(s) 148
SinI GGWCC 2 cut(s) 329, 567
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 2 cut(s) 102, 546
SsiI CCGC 1 cut(s) 536
SspI AATATT 1 cut(s) 28
SspMI CTAG 1 cut(s) 386
StyD4I CCNGG 2 cut(s) 211, 240
TaaI ACNGT 2 cut(s) 333, 365
TaqI TCGA 1 cut(s) 232
TasI AATT 2 cut(s) 102, 546
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 2 cut(s) 134, 269
Tru1I TTAA 2 cut(s) 30, 318
Tru9I TTAA 2 cut(s) 30, 318
TscAI CASTG 4 cut(s) 231, 271, 310, 352
TseI GCWGC 1 cut(s) 584
TspDTI ATGAA 2 cut(s) 331, 541
TspGWI ACGGA 1 cut(s) 559
TspRI CASTG 4 cut(s) 231, 271, 310, 352
VpaK11BI GGWCC 2 cut(s) 329, 567
VspI ATTAAT 1 cut(s) 30
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.