FvH4_3g21980

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014590
Physical Location & Seq
Forward (+)
15032776 .. 15034231
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21980.t1

Sequence Viewer

Length: 588 bp
ATGGCCAAAAGCATTGCGTTTCCGATGTTGGGGCTTCTTTGTCTATTGCTGTTGCTGCAGAAGAGTGGTGCAACTGAATTTACAATAGGTTGGACTGTTCCTGATGCATCAACTCACTTTGATCAATGGGCAGCGAACAACAGATTTCAAATTGGTGACTCCATAGTGTTCAACTATTCTCCTGGCCAAGACTCAGTGCTTCGAGTCAGCCAGGACGACTACACAAATTGCAACACCGATGCAACAGCTGAGAAATTCAGCGATGGCCACTCTACCTTCAAGTTTAACCAATCTGGGCCATTCTACTTCATCAGTGGGAACAAAGATAACTGTGTCAAAAATGAGAAGCTGGTTGTGATTGTCCTGGCAGATAGAAACCAAAACAGCACTGGAACCCCTCCATCTCCTGCACCTTCTGGCGAAGCGTCTCCACCTCCTTCACCAGGGACTGTCCAGAGTAACCCGACTCCAGCTCCCGAAAGTGATCAAATCCCTCCTCCACCTCCTAGCAGTGCTTCTTCAATCTTCATCAGTTTTGTTGGATCCATTGGAGCAGTTGCTGCATATTCATCAGTTTTATTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

196

Amino Acids

20.69

Weight (kDa)

4.67

Isoelectric Point (pI)

48.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 31 - 113 2e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20570
fragaria_vesca FvH4_3g21980
malus_domestica MD03G1209600.v1.1 MD11G1224400.v1.1
prunus_persica Prupe.4G205500_v2.0.a1
pyrus_communis pycom03g15840 pycom11g19810
rosa_chinensis RchiOBHm_Chr5g0037831
rosa_laevigata RLG00000033815
rosa_multiflora Rmu_sc0005416.1_g000014
rosa_roxburghii Rroxscaffold_1G00043090
rosa_rugosa Rorug05G0168000
rosa_samantha Rh5AG256800 Rh5BG259200 Rh5CG292200 Rh5DG269100
rosa_wichuraiana Rw5G023810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 537, 550
AcoI YGGCCR 3 cut(s) 3, 184, 265
AcsI RAATTY 2 cut(s) 77, 254
AfiI CCNNNNNNNGG 2 cut(s) 29, 443
AgsI TTSAA 4 cut(s) 149, 172, 280, 522
AjnI CCWGG 4 cut(s) 181, 210, 363, 442
AluBI AGCT 3 cut(s) 248, 349, 473
AluI AGCT 3 cut(s) 248, 349, 473
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 2 cut(s) 537, 550
AlwNI CAGNNNCTG 2 cut(s) 449, 560
AoxI GGCC 4 cut(s) 3, 184, 265, 296
ApeKI GCWGC 3 cut(s) 55, 131, 560
ApoI RAATTY 2 cut(s) 77, 254
AspS9I GGNCC 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 167, 432
BalI TGGCCA 3 cut(s) 5, 186, 267
BamHI GGATCC 1 cut(s) 542
BbvI GCAGC 3 cut(s) 42, 143, 547
BccI CCATC 2 cut(s) 257, 409
BciT130I CCWGG 4 cut(s) 183, 212, 365, 444
BclI TGATCA 2 cut(s) 121, 484
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 1 cut(s) 507
BfmI CTRYAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 56, 132, 561
BlsI GCNGC 3 cut(s) 57, 133, 562
Bme1390I CCNGG 4 cut(s) 183, 212, 365, 444
BmgT120I GGNCC 1 cut(s) 296
BmiI GGNNCC 2 cut(s) 394, 544
BmrFI CCNGG 4 cut(s) 183, 212, 365, 444
BmsI GCATC 3 cut(s) 94, 116, 229
BpmI CTGGAG 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 443
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 443
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 4 cut(s) 183, 212, 365, 444
BseDI CCNNGG 1 cut(s) 443
BseLI CCNNNNNNNGG 2 cut(s) 29, 443
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 207, 240
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 3 cut(s) 42, 143, 547
BsgI GTGCAG 1 cut(s) 393
BshFI GGCC 4 cut(s) 5, 186, 267, 298
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 2 cut(s) 29, 443
BsmAI GTCTC 1 cut(s) 432
BsmBI CGTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 460
BsnI GGCC 4 cut(s) 5, 186, 267, 298
Bsp143I GATC 3 cut(s) 121, 484, 542
BspANI GGCC 4 cut(s) 5, 186, 267, 298
BspCNI CTCAG 2 cut(s) 206, 241
BspLI GGNNCC 2 cut(s) 394, 544
BspMAI CTGCAG 1 cut(s) 60
BspPI GGATC 2 cut(s) 537, 550
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 443
BssMI GATC 3 cut(s) 121, 484, 542
Bst2UI CCWGG 4 cut(s) 183, 212, 365, 444
Bst4CI ACNGT 3 cut(s) 97, 332, 451
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 560
BstDEI CTNAG 2 cut(s) 193, 249
BstENI CCTNNNNNAGG 1 cut(s) 441
BstKTI GATC 3 cut(s) 124, 487, 545
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 3 cut(s) 121, 484, 542
BstMWI GCNNNNNNNGC 2 cut(s) 55, 560
BstNI CCWGG 4 cut(s) 183, 212, 365, 444
BstSCI CCNGG 4 cut(s) 181, 210, 363, 442
BstSFI CTRYAG 1 cut(s) 56
BstV1I GCAGC 3 cut(s) 42, 143, 547
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
BsuRI GGCC 4 cut(s) 5, 186, 267, 298
BtgZI GCGATG 1 cut(s) 276
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 4 cut(s) 201, 319, 387, 517
CaiI CAGNNNCTG 2 cut(s) 449, 560
Cfr13I GGNCC 1 cut(s) 296
CseI GACGC 1 cut(s) 414
CviJI RGCY 9 cut(s) 5, 34, 186, 210, 248, 267, 298, 349, 473
CviKI_1 RGCY 9 cut(s) 5, 34, 186, 210, 248, 267, 298, 349, 473
DdeI CTNAG 2 cut(s) 193, 249
DpnI GATC 3 cut(s) 123, 486, 544
DpnII GATC 3 cut(s) 121, 484, 542
EaeI YGGCCR 3 cut(s) 3, 184, 265
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 441
EcoRII CCWGG 4 cut(s) 181, 210, 363, 442
EcoT22I ATGCAT 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 432
FaiI YATR 2 cut(s) 164, 565
FaqI GGGAC 1 cut(s) 460
FbaI TGATCA 2 cut(s) 121, 484
Fnu4HI GCNGC 3 cut(s) 56, 132, 561
Fsp4HI GCNGC 3 cut(s) 56, 132, 561
FspBI CTAG 1 cut(s) 507
GluI GCNGC 3 cut(s) 56, 132, 561
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 4 cut(s) 5, 186, 267, 298
HgaI GACGC 1 cut(s) 414
HinfI GANTC 4 cut(s) 158, 191, 204, 466
HphI GGTGA 2 cut(s) 167, 432
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 3 cut(s) 101, 454, 476
HpyAV CCTTC 3 cut(s) 286, 423, 447
HpyCH4III ACNGT 3 cut(s) 97, 332, 451
HpyCH4V TGCA 7 cut(s) 58, 71, 107, 231, 242, 410, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 560
HpyF3I CTNAG 2 cut(s) 193, 249
Ksp22I TGATCA 2 cut(s) 121, 484
Kzo9I GATC 3 cut(s) 121, 484, 542
LmnI GCTCC 2 cut(s) 478, 551
Lsp1109I GCAGC 3 cut(s) 42, 143, 547
LweI GCATC 3 cut(s) 94, 116, 229
MaeI CTAG 1 cut(s) 507
MaeIII GTNAC 2 cut(s) 155, 458
MalI GATC 3 cut(s) 123, 486, 544
MboI GATC 3 cut(s) 121, 484, 542
MboII GAAGA 3 cut(s) 73, 510, 517
MflI RGATCY 1 cut(s) 542
MlsI TGGCCA 3 cut(s) 5, 186, 267
MluCI AATT 4 cut(s) 77, 150, 226, 254
MluNI TGGCCA 3 cut(s) 5, 186, 267
MlyI GAGTC 4 cut(s) 152, 185, 213, 460
MmeI TCCRAC 2 cut(s) 71, 520
MnlI CCTC 5 cut(s) 408, 444, 504, 507, 513
Mox20I TGGCCA 3 cut(s) 5, 186, 267
Mph1103I ATGCAT 1 cut(s) 109
MscI TGGCCA 3 cut(s) 5, 186, 267
MseI TTAA 1 cut(s) 285
Msp20I TGGCCA 3 cut(s) 5, 186, 267
MspA1I CMGCKG 1 cut(s) 248
MspR9I CCNGG 4 cut(s) 183, 212, 365, 444
MvaI CCWGG 4 cut(s) 183, 212, 365, 444
MwoI GCNNNNNNNGC 2 cut(s) 55, 560
NdeII GATC 3 cut(s) 121, 484, 542
NlaIV GGNNCC 2 cut(s) 394, 544
NmuCI GTSAC 1 cut(s) 155
NsiI ATGCAT 1 cut(s) 109
PkrI GCNGC 3 cut(s) 57, 133, 562
PleI GAGTC 4 cut(s) 152, 185, 212, 460
PpsI GAGTC 4 cut(s) 152, 185, 212, 460
Psp6I CCWGG 4 cut(s) 181, 210, 363, 442
PspGI CCWGG 4 cut(s) 181, 210, 363, 442
PspN4I GGNNCC 2 cut(s) 394, 544
PspPI GGNCC 1 cut(s) 296
PstI CTGCAG 1 cut(s) 60
PstNI CAGNNNCTG 2 cut(s) 449, 560
PsuI RGATCY 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 248
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 56, 132, 561
Sau3AI GATC 3 cut(s) 121, 484, 542
Sau96I GGNCC 1 cut(s) 296
SchI GAGTC 4 cut(s) 152, 185, 213, 460
ScrFI CCNGG 4 cut(s) 183, 212, 365, 444
SetI ASST 8 cut(s) 91, 250, 278, 351, 415, 436, 475, 505
SfaNI GCATC 3 cut(s) 94, 116, 229
SfcI CTRYAG 1 cut(s) 56
Sse9I AATT 4 cut(s) 77, 150, 226, 254
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 4 cut(s) 181, 210, 363, 442
TaaI ACNGT 3 cut(s) 97, 332, 451
TaqI TCGA 1 cut(s) 202
TasI AATT 4 cut(s) 77, 150, 226, 254
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 4 cut(s) 201, 319, 394, 517
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 3 cut(s) 55, 131, 560
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 3 cut(s) 298, 517, 558
TspRI CASTG 4 cut(s) 201, 319, 394, 517
XagI CCTNNNNNAGG 1 cut(s) 441
XapI RAATTY 2 cut(s) 77, 254
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XspI CTAG 1 cut(s) 507
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.