FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G24200 AT3G24200 |
| fragaria_vesca | FvH4_5g03680 FvH4_5g03680 FvH4_5g03680 |
| malus_domestica | MD06G1096300.v1.1 MD14G1116500.v1.1 |
| prunus_persica | Prupe.5G110900_v2.0.a1 |
| pyrus_communis | pycom06g09260 |
| rosa_chinensis | RchiOBHm_Chr7g0196051 |
| rosa_laevigata | RLG00000004070 |
| rosa_multiflora | Rmu_sc0001640.1_g000009 |
| rosa_roxburghii | Rroxscaffold_3G00259900 |
| rosa_rugosa | Rorug07G0029300 Rorug07G0029400 |
| rosa_samantha | Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900 |
| rosa_wichuraiana | Rw7G013330 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 340 |
| Acc36I | ACCTGC | 3 cut(s) | 340, 403, 1348 |
| AccB1I | GGYRCC | 1 cut(s) | 362 |
| AccI | GTMKAC | 1 cut(s) | 693 |
| AccII | CGCG | 3 cut(s) | 566, 690, 733 |
| AciI | CCGC | 5 cut(s) | 564, 1361, 1406, 1457, 1505 |
| AclI | AACGTT | 1 cut(s) | 1318 |
| AclWI | GGATC | 2 cut(s) | 302, 761 |
| AcoI | YGGCCR | 1 cut(s) | 210 |
| AcuI | CTGAAG | 3 cut(s) | 491, 1092, 1470 |
| AcyI | GRCGYC | 1 cut(s) | 655 |
| AfaI | GTAC | 7 cut(s) | 322, 465, 531, 624, 799, 1288, 1388 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 269, 919, 1071, 1211 |
| AflIII | ACRYGT | 1 cut(s) | 1165 |
| AgsI | TTSAA | 2 cut(s) | 238, 1079 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1156 |
| AjnI | CCWGG | 1 cut(s) | 365 |
| Alw21I | GWGCWC | 2 cut(s) | 15, 1252 |
| Alw26I | GTCTC | 2 cut(s) | 1001, 1034 |
| AlwI | GGATC | 2 cut(s) | 302, 761 |
| AoxI | GGCC | 3 cut(s) | 210, 430, 853 |
| ApeKI | GCWGC | 3 cut(s) | 200, 1424, 1427 |
| AspLEI | GCGC | 1 cut(s) | 716 |
| AspS9I | GGNCC | 6 cut(s) | 146, 854, 903, 910, 995, 1403 |
| AsuHPI | GGTGA | 4 cut(s) | 629, 890, 1193, 1193 |
| AvaII | GGWCC | 5 cut(s) | 146, 903, 910, 995, 1403 |
| BalI | TGGCCA | 1 cut(s) | 212 |
| BanI | GGYRCC | 1 cut(s) | 362 |
| BanII | GRGCYC | 1 cut(s) | 1444 |
| BbsI | GAAGAC | 1 cut(s) | 557 |
| Bbv12I | GWGCWC | 2 cut(s) | 15, 1252 |
| BbvI | GCAGC | 3 cut(s) | 187, 1414, 1436 |
| BccI | CCATC | 7 cut(s) | 137, 574, 634, 674, 713, 762, 1351 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 568, 602 |
| BciT130I | CCWGG | 1 cut(s) | 367 |
| BcoDI | GTCTC | 2 cut(s) | 1001, 1034 |
| BfaI | CTAG | 2 cut(s) | 594, 1229 |
| BfmI | CTRYAG | 1 cut(s) | 1263 |
| BfoI | RGCGCY | 1 cut(s) | 717 |
| BfuAI | ACCTGC | 3 cut(s) | 340, 403, 1348 |
| BglII | AGATCT | 1 cut(s) | 541 |
| BisI | GCNGC | 5 cut(s) | 201, 1425, 1428, 1457, 1505 |
| BlpI | GCTNAGC | 2 cut(s) | 663, 674 |
| BlsI | GCNGC | 5 cut(s) | 202, 1426, 1429, 1458, 1506 |
| Bme1390I | CCNGG | 1 cut(s) | 367 |
| Bme18I | GGWCC | 5 cut(s) | 146, 903, 910, 995, 1403 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1156 |
| BmgT120I | GGNCC | 6 cut(s) | 146, 854, 903, 910, 995, 1403 |
| BmiI | GGNNCC | 2 cut(s) | 364, 912 |
| BmrFI | CCNGG | 1 cut(s) | 367 |
| BmsI | GCATC | 6 cut(s) | 307, 399, 932, 964, 1039, 1231 |
| BoxI | GACNNNNGTC | 2 cut(s) | 35, 144 |
| BpiI | GAAGAC | 1 cut(s) | 557 |
| BpmI | CTGGAG | 2 cut(s) | 530, 689 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 663, 674 |
| BsaBI | GATNNNNATC | 2 cut(s) | 465, 684 |
| BsaHI | GRCGYC | 1 cut(s) | 655 |
| BsaJI | CCNNGG | 4 cut(s) | 433, 906, 1089, 1435 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 269, 919, 1071, 1211 |
| Bse1I | ACTGG | 2 cut(s) | 708, 769 |
| Bse3DI | GCAATG | 4 cut(s) | 124, 159, 1275, 1359 |
| Bse8I | GATNNNNATC | 2 cut(s) | 465, 684 |
| BseBI | CCWGG | 1 cut(s) | 367 |
| BseDI | CCNNGG | 4 cut(s) | 433, 906, 1089, 1435 |
| BseGI | GGATG | 3 cut(s) | 626, 773, 955 |
| BseJI | GATNNNNATC | 2 cut(s) | 465, 684 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 269, 919, 1071, 1211 |
| BseMI | GCAATG | 4 cut(s) | 124, 159, 1275, 1359 |
| BseMII | CTCAG | 2 cut(s) | 530, 1200 |
| BseNI | ACTGG | 2 cut(s) | 708, 769 |
| BseRI | GAGGAG | 1 cut(s) | 1074 |
| BseXI | GCAGC | 3 cut(s) | 187, 1414, 1436 |
| BseYI | CCCAGC | 1 cut(s) | 749 |
| Bsh1236I | CGCG | 3 cut(s) | 566, 690, 733 |
| BshFI | GGCC | 3 cut(s) | 212, 432, 855 |
| BshNI | GGYRCC | 1 cut(s) | 362 |
| BsiHKAI | GWGCWC | 2 cut(s) | 15, 1252 |
| BslFI | GGGAC | 1 cut(s) | 434 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 269, 919, 1071, 1211 |
| BsmAI | GTCTC | 2 cut(s) | 1001, 1034 |
| BsmFI | GGGAC | 1 cut(s) | 434 |
| BsmI | GAATGC | 2 cut(s) | 1417, 1430 |
| BsnI | GGCC | 3 cut(s) | 212, 432, 855 |
| Bsp1286I | GDGCHC | 3 cut(s) | 15, 1252, 1444 |
| Bsp1407I | TGTACA | 2 cut(s) | 463, 529 |
| Bsp143I | GATC | 4 cut(s) | 307, 541, 753, 928 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 663, 674 |
| Bsp19I | CCATGG | 1 cut(s) | 906 |
| Bsp68I | TCGCGA | 1 cut(s) | 733 |
| BspACI | CCGC | 5 cut(s) | 564, 1361, 1406, 1457, 1505 |
| BspANI | GGCC | 3 cut(s) | 212, 432, 855 |
| BspCNI | CTCAG | 2 cut(s) | 529, 1199 |
| BspFNI | CGCG | 3 cut(s) | 566, 690, 733 |
| BspLI | GGNNCC | 2 cut(s) | 364, 912 |
| BspMI | ACCTGC | 3 cut(s) | 340, 403, 1348 |
| BspPI | GGATC | 2 cut(s) | 302, 761 |
| BspQI | GCTCTTC | 1 cut(s) | 604 |
| BspT107I | GGYRCC | 1 cut(s) | 362 |
| BsrDI | GCAATG | 4 cut(s) | 124, 159, 1275, 1359 |
| BsrGI | TGTACA | 2 cut(s) | 463, 529 |
| BsrI | ACTGG | 2 cut(s) | 708, 769 |
| BssECI | CCNNGG | 4 cut(s) | 433, 906, 1089, 1435 |
| BssMI | GATC | 4 cut(s) | 307, 541, 753, 928 |
| BssNI | GRCGYC | 1 cut(s) | 655 |
| BssT1I | CCWWGG | 3 cut(s) | 433, 906, 1089 |
| Bst2UI | CCWGG | 1 cut(s) | 367 |
| Bst4CI | ACNGT | 6 cut(s) | 76, 325, 556, 802, 840, 1198 |
| Bst6I | CTCTTC | 1 cut(s) | 604 |
| BstACI | GRCGYC | 1 cut(s) | 655 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1501 |
| BstAUI | TGTACA | 2 cut(s) | 463, 529 |
| BstC8I | GCNNGC | 4 cut(s) | 15, 214, 218, 1143 |
| BstDEI | CTNAG | 7 cut(s) | 26, 516, 663, 674, 726, 1186, 1418 |
| BstDSI | CCRYGG | 2 cut(s) | 906, 1435 |
| BstF5I | GGATG | 3 cut(s) | 626, 773, 955 |
| BstFNI | CGCG | 3 cut(s) | 566, 690, 733 |
| BstH2I | RGCGCY | 1 cut(s) | 717 |
| BstHHI | GCGC | 1 cut(s) | 716 |
| BstKTI | GATC | 4 cut(s) | 310, 544, 756, 931 |
| BstMAI | GTCTC | 2 cut(s) | 1001, 1034 |
| BstMBI | GATC | 4 cut(s) | 307, 541, 753, 928 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 200, 209, 1349, 1358, 1421, 1465, 1501 |
| BstNI | CCWGG | 1 cut(s) | 367 |
| BstNSI | RCATGY | 1 cut(s) | 395 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 35, 144 |
| BstSCI | CCNGG | 1 cut(s) | 365 |
| BstSFI | CTRYAG | 1 cut(s) | 1263 |
| BstUI | CGCG | 3 cut(s) | 566, 690, 733 |
| BstV1I | GCAGC | 3 cut(s) | 187, 1414, 1436 |
| BstV2I | GAAGAC | 1 cut(s) | 557 |
| BstX2I | RGATCY | 1 cut(s) | 541 |
| BstYI | RGATCY | 1 cut(s) | 541 |
| BsuRI | GGCC | 3 cut(s) | 212, 432, 855 |
| BtgI | CCRYGG | 2 cut(s) | 906, 1435 |
| BtgZI | GCGATG | 2 cut(s) | 593, 693 |
| BtsCI | GGATG | 3 cut(s) | 626, 773, 955 |
| BtsIMutI | CAGTG | 1 cut(s) | 135 |
| BtuMI | TCGCGA | 1 cut(s) | 733 |
| BveI | ACCTGC | 3 cut(s) | 340, 403, 1348 |
| Cac8I | GCNNGC | 4 cut(s) | 15, 214, 218, 1143 |
| CfoI | GCGC | 1 cut(s) | 716 |
| Cfr13I | GGNCC | 6 cut(s) | 146, 854, 903, 910, 995, 1403 |
| CpoI | CGGWCCG | 1 cut(s) | 1403 |
| CseI | GACGC | 2 cut(s) | 663, 679 |
| Csp6I | GTAC | 7 cut(s) | 321, 464, 530, 623, 798, 1287, 1387 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 687, 722, 1149, 1184 |
| CspI | CGGWCCG | 1 cut(s) | 1403 |
| CviAII | CATG | 6 cut(s) | 86, 155, 392, 646, 831, 907 |
| CviQI | GTAC | 7 cut(s) | 321, 464, 530, 623, 798, 1287, 1387 |
| DdeI | CTNAG | 7 cut(s) | 26, 516, 663, 674, 726, 1186, 1418 |
| DpnI | GATC | 4 cut(s) | 309, 543, 755, 930 |
| DpnII | GATC | 4 cut(s) | 307, 541, 753, 928 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1156 |
| EaeI | YGGCCR | 1 cut(s) | 210 |
| Eam1104I | CTCTTC | 1 cut(s) | 604 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1156 |
| EarI | CTCTTC | 1 cut(s) | 604 |
| Eco130I | CCWWGG | 3 cut(s) | 433, 906, 1089 |
| Eco147I | AGGCCT | 1 cut(s) | 432 |
| Eco24I | GRGCYC | 1 cut(s) | 1444 |
| Eco47I | GGWCC | 5 cut(s) | 146, 903, 910, 995, 1403 |
| Eco57I | CTGAAG | 3 cut(s) | 491, 1092, 1470 |
| EcoRII | CCWGG | 1 cut(s) | 365 |
| EcoT14I | CCWWGG | 3 cut(s) | 433, 906, 1089 |
| EcoT22I | ATGCAT | 1 cut(s) | 124 |
| EcoT38I | GRGCYC | 1 cut(s) | 1444 |
| ErhI | CCWWGG | 3 cut(s) | 433, 906, 1089 |
| FaeI | CATG | 6 cut(s) | 89, 158, 395, 649, 834, 910 |
| FaqI | GGGAC | 1 cut(s) | 434 |
| FatI | CATG | 6 cut(s) | 85, 154, 391, 645, 830, 906 |
| FauI | CCCGC | 1 cut(s) | 571 |
| FblI | GTMKAC | 1 cut(s) | 693 |
| Fnu4HI | GCNGC | 5 cut(s) | 201, 1425, 1428, 1457, 1505 |
| FokI | GGATG | 3 cut(s) | 613, 780, 962 |
| FriOI | GRGCYC | 1 cut(s) | 1444 |
| Fsp4HI | GCNGC | 5 cut(s) | 201, 1425, 1428, 1457, 1505 |
| FspBI | CTAG | 2 cut(s) | 594, 1229 |
| GlaI | GCGC | 1 cut(s) | 715 |
| GluI | GCNGC | 5 cut(s) | 201, 1425, 1428, 1457, 1505 |
| GsaI | CCCAGC | 1 cut(s) | 753 |
| GsuI | CTGGAG | 2 cut(s) | 530, 689 |
| HaeII | RGCGCY | 1 cut(s) | 717 |
| HaeIII | GGCC | 3 cut(s) | 212, 432, 855 |
| HgaI | GACGC | 2 cut(s) | 663, 679 |
| HhaI | GCGC | 1 cut(s) | 716 |
| Hin1I | GRCGYC | 1 cut(s) | 655 |
| Hin1II | CATG | 6 cut(s) | 89, 158, 395, 649, 834, 910 |
| Hin6I | GCGC | 1 cut(s) | 714 |
| HinP1I | GCGC | 1 cut(s) | 714 |
| HincII | GTYRAC | 1 cut(s) | 606 |
| HindII | GTYRAC | 1 cut(s) | 606 |
| HindIII | AAGCTT | 1 cut(s) | 1100 |
| HinfI | GANTC | 8 cut(s) | 22, 189, 315, 536, 983, 1184, 1306, 1374 |
| HphI | GGTGA | 4 cut(s) | 629, 890, 1193, 1193 |
| Hpy166II | GTNNAC | 6 cut(s) | 149, 530, 606, 694, 1019, 1201 |
| Hpy188I | TCNGA | 5 cut(s) | 510, 541, 928, 1111, 1403 |
| Hpy188III | TCNNGA | 9 cut(s) | 470, 547, 732, 980, 987, 1004, 1378, 1488, 1515 |
| Hpy8I | GTNNAC | 6 cut(s) | 149, 530, 606, 694, 1019, 1201 |
| HpyAV | CCTTC | 1 cut(s) | 355 |
| HpyCH4III | ACNGT | 6 cut(s) | 76, 325, 556, 802, 840, 1198 |
| HpyCH4IV | ACGT | 2 cut(s) | 1165, 1318 |
| HpyCH4V | TGCA | 7 cut(s) | 58, 122, 335, 412, 1238, 1343, 1430 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 200, 209, 1349, 1358, 1421, 1465, 1501 |
| HpyF3I | CTNAG | 7 cut(s) | 26, 516, 663, 674, 726, 1186, 1418 |
| HpySE526I | ACGT | 2 cut(s) | 1165, 1318 |
| Hsp92I | GRCGYC | 1 cut(s) | 655 |
| Hsp92II | CATG | 6 cut(s) | 89, 158, 395, 649, 834, 910 |
| HspAI | GCGC | 1 cut(s) | 714 |
| Kzo9I | GATC | 4 cut(s) | 307, 541, 753, 928 |
| LguI | GCTCTTC | 1 cut(s) | 604 |
| LmnI | GCTCC | 1 cut(s) | 670 |
| Lsp1109I | GCAGC | 3 cut(s) | 187, 1414, 1436 |
| LweI | GCATC | 6 cut(s) | 307, 399, 932, 964, 1039, 1231 |
| MaeI | CTAG | 2 cut(s) | 594, 1229 |
| MaeII | ACGT | 2 cut(s) | 1165, 1318 |
| MaeIII | GTNAC | 4 cut(s) | 79, 325, 878, 1181 |
| MalI | GATC | 4 cut(s) | 309, 543, 755, 930 |
| MboI | GATC | 4 cut(s) | 307, 541, 753, 928 |
| MboII | GAAGA | 7 cut(s) | 288, 497, 562, 591, 860, 1098, 1393 |
| MflI | RGATCY | 1 cut(s) | 541 |
| MhlI | GDGCHC | 3 cut(s) | 15, 1252, 1444 |
| MlsI | TGGCCA | 1 cut(s) | 212 |
| MluCI | AATT | 3 cut(s) | 130, 792, 935 |
| MluNI | TGGCCA | 1 cut(s) | 212 |
| MlyI | GAGTC | 2 cut(s) | 324, 1178 |
| MmeI | TCCRAC | 2 cut(s) | 166, 1374 |
| Mox20I | TGGCCA | 1 cut(s) | 212 |
| Mph1103I | ATGCAT | 1 cut(s) | 124 |
| MscI | TGGCCA | 1 cut(s) | 212 |
| MseI | TTAA | 4 cut(s) | 125, 572, 1136, 1461 |
| MslI | CAYNNNNRTG | 2 cut(s) | 237, 471 |
| Msp20I | TGGCCA | 1 cut(s) | 212 |
| MspA1I | CMGCKG | 2 cut(s) | 1408, 1427 |
| MspR9I | CCNGG | 1 cut(s) | 367 |
| Mva1269I | GAATGC | 2 cut(s) | 1417, 1430 |
| MvaI | CCWGG | 1 cut(s) | 367 |
| MvnI | CGCG | 3 cut(s) | 566, 690, 733 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 200, 209, 1349, 1358, 1421, 1465, 1501 |
| NcoI | CCATGG | 1 cut(s) | 906 |
| NdeII | GATC | 4 cut(s) | 307, 541, 753, 928 |
| NlaIII | CATG | 6 cut(s) | 89, 158, 395, 649, 834, 910 |
| NlaIV | GGNNCC | 2 cut(s) | 364, 912 |
| NmuCI | GTSAC | 4 cut(s) | 79, 325, 878, 1181 |
| NruI | TCGCGA | 1 cut(s) | 733 |
| NsiI | ATGCAT | 1 cut(s) | 124 |
| NspI | RCATGY | 1 cut(s) | 395 |
| PaqCI | CACCTGC | 1 cut(s) | 340 |
| PceI | AGGCCT | 1 cut(s) | 432 |
| PciSI | GCTCTTC | 1 cut(s) | 604 |
| PctI | GAATGC | 2 cut(s) | 1417, 1430 |
| PfeI | GAWTC | 6 cut(s) | 22, 189, 536, 983, 1306, 1374 |
| PkrI | GCNGC | 5 cut(s) | 202, 1426, 1429, 1458, 1506 |
| PleI | GAGTC | 2 cut(s) | 323, 1178 |
| PpsI | GAGTC | 2 cut(s) | 323, 1178 |
| PshAI | GACNNNNGTC | 2 cut(s) | 35, 144 |
| Psp1406I | AACGTT | 1 cut(s) | 1318 |
| Psp6I | CCWGG | 1 cut(s) | 365 |
| PspFI | CCCAGC | 1 cut(s) | 749 |
| PspGI | CCWGG | 1 cut(s) | 365 |
| PspN4I | GGNNCC | 2 cut(s) | 364, 912 |
| PspPI | GGNCC | 6 cut(s) | 146, 854, 903, 910, 995, 1403 |
| PsuI | RGATCY | 1 cut(s) | 541 |
| PvuII | CAGCTG | 1 cut(s) | 1427 |
| RruI | TCGCGA | 1 cut(s) | 733 |
| RsaI | GTAC | 7 cut(s) | 322, 465, 531, 624, 799, 1288, 1388 |
| RsaNI | GTAC | 7 cut(s) | 321, 464, 530, 623, 798, 1287, 1387 |
| RseI | CAYNNNNRTG | 2 cut(s) | 237, 471 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1403 |
| RsrII | CGGWCCG | 1 cut(s) | 1403 |
| SapI | GCTCTTC | 1 cut(s) | 604 |
| SaqAI | TTAA | 4 cut(s) | 125, 572, 1136, 1461 |
| SatI | GCNGC | 5 cut(s) | 201, 1425, 1428, 1457, 1505 |
| Sau3AI | GATC | 4 cut(s) | 307, 541, 753, 928 |
| Sau96I | GGNCC | 6 cut(s) | 146, 854, 903, 910, 995, 1403 |
| SchI | GAGTC | 2 cut(s) | 324, 1178 |
| ScrFI | CCNGG | 1 cut(s) | 367 |
| SduI | GDGCHC | 3 cut(s) | 15, 1252, 1444 |
| SfaNI | GCATC | 6 cut(s) | 307, 399, 932, 964, 1039, 1231 |
| SfcI | CTRYAG | 1 cut(s) | 1263 |
| SinI | GGWCC | 5 cut(s) | 146, 903, 910, 995, 1403 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 237, 471 |
| Sse9I | AATT | 3 cut(s) | 130, 792, 935 |
| SseBI | AGGCCT | 1 cut(s) | 432 |
| SsiI | CCGC | 5 cut(s) | 564, 1361, 1406, 1457, 1505 |
| SspMI | CTAG | 2 cut(s) | 594, 1229 |
| StuI | AGGCCT | 1 cut(s) | 432 |
| StyD4I | CCNGG | 1 cut(s) | 365 |
| StyI | CCWWGG | 3 cut(s) | 433, 906, 1089 |
| TaaI | ACNGT | 6 cut(s) | 76, 325, 556, 802, 840, 1198 |
| TaiI | ACGT | 2 cut(s) | 1168, 1321 |
| TaqI | TCGA | 2 cut(s) | 71, 931 |
| TasI | AATT | 3 cut(s) | 130, 792, 935 |
| TatI | WGTACW | 5 cut(s) | 320, 463, 529, 797, 1386 |
| TauI | GCSGC | 2 cut(s) | 1459, 1507 |
| TfiI | GAWTC | 6 cut(s) | 22, 189, 536, 983, 1306, 1374 |
| Tru1I | TTAA | 4 cut(s) | 125, 572, 1136, 1461 |
| Tru9I | TTAA | 4 cut(s) | 125, 572, 1136, 1461 |
| TscAI | CASTG | 1 cut(s) | 142 |
| TseFI | GTSAC | 4 cut(s) | 79, 325, 878, 1181 |
| TseI | GCWGC | 3 cut(s) | 200, 1424, 1427 |
| Tsp45I | GTSAC | 4 cut(s) | 79, 325, 878, 1181 |
| TspDTI | ATGAA | 6 cut(s) | 17, 195, 634, 946, 956, 1338 |
| TspGWI | ACGGA | 1 cut(s) | 1386 |
| TspRI | CASTG | 1 cut(s) | 142 |
| VpaK11BI | GGWCC | 5 cut(s) | 146, 903, 910, 995, 1403 |
| XceI | RCATGY | 1 cut(s) | 395 |
| XmiI | GTMKAC | 1 cut(s) | 693 |
| XspI | CTAG | 2 cut(s) | 594, 1229 |
| Zsp2I | ATGCAT | 1 cut(s) | 124 |