MD06G1096300.v1.1

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011500
Physical Location & Seq
Forward (+)
23047123 .. 23051710
4588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1096300.v1.1.491

Sequence Viewer

Length: 1530 bp
ATGAACAGCTTGATTATCAGAAGAAATCTTGCTGAAAGTATCAAAAGATTGAAGCTTTCTCAAAGGGCTTTGTGTACGGACGCAGCAGCAAAAGTTTCCAGCTCCAATGGTGCCCAACCCGGCAATGGCCGTGTGGCGCAGCCGATTTTTTCGAATAAAAGCAATCCGTATGACATTGCTATTGTTGGAGGAGGGATGGTTGGCATGGCTCTTGCTTGTTCCTTGGCAAAAATGCCGTTGACAAAGCACTTGAAAGTTGCCATCATTGATAGCAATCCCGCAGTATCAAATGGGCTTAGCATCAAGAAAGAAGACCCCCCTGATCCAAGGGTCAGTACAGTAACACCTGTGACCATATCTCTCTTCAAAGATATTGGCGCTTGGAAATATGTGGAACAGAATAGACATGCATATTTTGATCAAATGCAGGTTTGGGACTATACTGGCTTAGGATATACAAGGTACGATGCAAGAGATGTACATAAAGATTCTCTGGGGTGTGTAGTGGAGAATAAAGTACTGCACAGTTCTCTATTGTCATGTATGCAGAATACAGATTTCCAGAAGACTATCTACCCTTCCAGATTGTCCACAATGACTTTACAGCCAAGGAACTTATCCATGGGGGTGGGAAGCACATCATCTGGATTGAATGAGCAGGGGAACTTAGCAAAGCTTGATCTAATTGACGGCAACAGTCTATATGCAAAGTTGGTGGTTGGAGCTGATGGGTCCAAGTCACGTGTTAGAGAGTTGGCAGGATTTAAGACAACCGGATGGAGTTACTCACAGAATGCAATCATTTGTACTGTTGAACATAGTGTGGAAAACCGATGTGCATGGCAACGCTTTCTACCTGCTGGGCCAATTGCACTTTTGCCAATAGGTGATAATTTCAGCAATATTGTTTGGACTATGAAACCGAAAGAAGCAACTGACCGTAAATTAAAGGCTGAGGATGAATTTGTGAAAGATGTCAATTATGCTCTGGATTATGGATTTGGTCCACATCCTGGGTCAAGCACCTCAGGAGGTGGAGGCATATTTTCTTTGTTTACAAACGCGAATTTATCTGCCAACGATTGCTTCAAGGTTCCACCGAAAGTGTTGAAGTTGGTGTCTGAAAGAATGGTGTTTCCATTGTCTTTGATGCATGCCAACAACTACGCGTCAAAGCATGTGGTTCTAATCGGCGATGCAGCACATACCGTTCACCCTTTGGCTGGACAAGGGGTGAATTTGGGTTTTGGAGACGCGTTTGCTCTTTCAAGAATCATTTCGGAGGGAATTGCAGTGGGAAGAGATATTGCCGAGGTATCGTTGTTGAAGAAATACGAAGCAGAGCGGAAATCAGCAAATGTCACAATGATGGCAATCCTGGACGGGTTCCAGAAGGCTTACTCCGTTGATTTCGGTCCTTTAAATGTTATTCGGGCAGCTGCATTCAATGGAGCACAGTACTTCCCACCCCTTAAGAGGAGTATTATTTCATACGCATCAGGGGATCAAAGATTGCCAATTTTCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

510

Amino Acids

55.21

Weight (kDa)

9.21

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 58 - 323 7.4e-08 FAD binding domain
FAD_binding_3 PF01494 373 - 452 2.7e-13 FAD binding domain
SE PF08491 379 - 483 1.2e-07 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 418, 865
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 1013
AccBSI CCGCTC 1 cut(s) 1345
AccII CGCG 3 cut(s) 1064, 1169, 1256
AciI CCGC 2 cut(s) 279, 1345
AclWI GGATC 2 cut(s) 317, 1512
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 3 cut(s) 962, 1066, 1237
AcvI CACGTG 1 cut(s) 743
AfaI GTAC 7 cut(s) 76, 337, 464, 480, 519, 808, 1460
AfiI CCNNNNNNNGG 4 cut(s) 125, 1013, 1223, 1476
AflII CTTAAG 1 cut(s) 1472
AflIII ACRYGT 3 cut(s) 742, 1167, 1254
AjnI CCWGG 2 cut(s) 1012, 1377
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 6 cut(s) 9, 55, 102, 676, 725, 1439
AluI AGCT 6 cut(s) 9, 55, 102, 676, 725, 1439
Alw21I GWGCWC 1 cut(s) 1456
Alw26I GTCTC 1 cut(s) 1245
AlwI GGATC 2 cut(s) 317, 1512
AoxI GGCC 2 cut(s) 127, 863
ApeKI GCWGC 6 cut(s) 83, 86, 139, 1199, 1436, 1439
ApoI RAATTY 3 cut(s) 962, 1066, 1237
Asp700I GAANNNNTTC 1 cut(s) 1276
AspLEI GCGC 2 cut(s) 139, 380
AspS9I GGNCC 4 cut(s) 732, 863, 1004, 1415
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 3 cut(s) 899, 1205, 1246
AsuII TTCGAA 1 cut(s) 152
AvaII GGWCC 3 cut(s) 732, 1004, 1415
AxyI CCTNAGG 1 cut(s) 1027
BaeGI GKGCMC 1 cut(s) 115
BanI GGYRCC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 557, 589
BbrPI CACGTG 1 cut(s) 743
BbsI GAAGAC 2 cut(s) 318, 572
Bbv12I GWGCWC 1 cut(s) 1456
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 6 cut(s) 95, 98, 151, 1211, 1426, 1448
BccI CCATC 5 cut(s) 190, 269, 722, 771, 1363
BceAI ACGGC 3 cut(s) 114, 220, 706
BciT130I CCWGG 2 cut(s) 1014, 1379
BclI TGATCA 1 cut(s) 418
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 1245
BfoI RGCGCY 1 cut(s) 381
BfrI CTTAAG 1 cut(s) 1472
BfuAI ACCTGC 2 cut(s) 418, 865
BisI GCNGC 6 cut(s) 84, 87, 140, 1200, 1437, 1440
BlpI GCTNAGC 1 cut(s) 296
BlsI GCNGC 6 cut(s) 85, 88, 141, 1201, 1438, 1441
BmcAI AGTACT 2 cut(s) 519, 1460
Bme1390I CCNGG 3 cut(s) 120, 1014, 1379
Bme18I GGWCC 3 cut(s) 732, 1004, 1415
BmgT120I GGNCC 4 cut(s) 732, 863, 1004, 1415
BmiI GGNNCC 4 cut(s) 112, 733, 1095, 1388
BmrFI CCNGG 3 cut(s) 120, 1014, 1379
BmsI GCATC 5 cut(s) 309, 457, 1140, 1186, 1505
BpiI GAAGAC 2 cut(s) 318, 572
Bpu10I CCTNAGC 2 cut(s) 448, 954
Bpu1102I GCTNAGC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 120
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 2 cut(s) 273, 1373
BsaJI CCNNGG 6 cut(s) 222, 326, 608, 621, 1013, 1311
BsaWI WCCGGW 1 cut(s) 773
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 1013, 1223, 1476
Bse1I ACTGG 1 cut(s) 448
Bse21I CCTNAGG 1 cut(s) 1027
Bse3DI GCAATG 2 cut(s) 130, 174
Bse8I GATNNNNATC 2 cut(s) 273, 1373
BseBI CCWGG 2 cut(s) 1014, 1379
BseDI CCNNGG 6 cut(s) 222, 326, 608, 621, 1013, 1311
BseGI GGATG 4 cut(s) 201, 782, 964, 1009
BseJI GATNNNNATC 2 cut(s) 273, 1373
BseLI CCNNNNNNNGG 4 cut(s) 125, 1013, 1223, 1476
BseMI GCAATG 2 cut(s) 130, 174
BseMII CTCAG 2 cut(s) 945, 1041
BseNI ACTGG 1 cut(s) 448
BseRI GAGGAG 2 cut(s) 204, 1492
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 6 cut(s) 95, 98, 151, 1211, 1426, 1448
BseYI CCCAGC 1 cut(s) 860
BsgI GTGCAG 1 cut(s) 506
Bsh1236I CGCG 3 cut(s) 1064, 1169, 1256
BshFI GGCC 2 cut(s) 129, 865
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 1456
BsiSI CCGG 2 cut(s) 120, 774
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 4 cut(s) 125, 1013, 1223, 1476
BsmAI GTCTC 1 cut(s) 1245
BsmBI CGTCTC 1 cut(s) 1245
BsmFI GGGAC 1 cut(s) 449
BsmI GAATGC 2 cut(s) 799, 1442
BsnI GGCC 2 cut(s) 129, 865
Bsp119I TTCGAA 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 115, 1456
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 4 cut(s) 322, 418, 679, 1504
Bsp1720I GCTNAGC 1 cut(s) 296
Bsp19I CCATGG 1 cut(s) 621
BspACI CCGC 2 cut(s) 279, 1345
BspANI GGCC 2 cut(s) 129, 865
BspCNI CTCAG 2 cut(s) 946, 1040
BspFNI CGCG 3 cut(s) 1064, 1169, 1256
BspLI GGNNCC 4 cut(s) 112, 733, 1095, 1388
BspMI ACCTGC 2 cut(s) 418, 865
BspPI GGATC 2 cut(s) 317, 1512
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 1472
BsrBI CCGCTC 1 cut(s) 1345
BsrDI GCAATG 2 cut(s) 130, 174
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 1 cut(s) 448
BssECI CCNNGG 6 cut(s) 222, 326, 608, 621, 1013, 1311
BssMI GATC 4 cut(s) 322, 418, 679, 1504
BssT1I CCWWGG 4 cut(s) 222, 326, 608, 621
Bst2UI CCWGG 2 cut(s) 1014, 1379
Bst4CI ACNGT 7 cut(s) 340, 527, 698, 811, 941, 1210, 1458
Bst6I CTCTTC 2 cut(s) 368, 1294
BstAFI CTTAAG 1 cut(s) 1472
BstAUI TGTACA 1 cut(s) 478
BstBAI YACGTR 1 cut(s) 743
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 1155
BstDEI CTNAG 6 cut(s) 296, 448, 667, 954, 1027, 1527
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 4 cut(s) 201, 782, 964, 1009
BstFNI CGCG 3 cut(s) 1064, 1169, 1256
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 2 cut(s) 139, 380
BstKTI GATC 4 cut(s) 325, 421, 682, 1507
BstMAI GTCTC 1 cut(s) 1245
BstMBI GATC 4 cut(s) 322, 418, 679, 1504
BstNI CCWGG 2 cut(s) 1014, 1379
BstNSI RCATGY 3 cut(s) 410, 1157, 1181
BstSCI CCNGG 3 cut(s) 118, 1012, 1377
BstSLI GKGCMC 1 cut(s) 115
BstUI CGCG 3 cut(s) 1064, 1169, 1256
BstV1I GCAGC 6 cut(s) 95, 98, 151, 1211, 1426, 1448
BstV2I GAAGAC 2 cut(s) 318, 572
BstXI CCANNNNNNTGG 1 cut(s) 628
Bsu36I CCTNAGG 1 cut(s) 1027
BsuRI GGCC 2 cut(s) 129, 865
BtgI CCRYGG 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 1209
BtsCI GGATG 4 cut(s) 201, 782, 964, 1009
BtsI GCAGTG 1 cut(s) 1299
BtsIMutI CAGTG 1 cut(s) 1299
BveI ACCTGC 2 cut(s) 418, 865
Cac8I GCNNGC 1 cut(s) 1155
CfoI GCGC 2 cut(s) 139, 380
Cfr13I GGNCC 4 cut(s) 732, 863, 1004, 1415
CseI GACGC 3 cut(s) 89, 1158, 1262
Csp6I GTAC 7 cut(s) 75, 336, 463, 479, 518, 807, 1459
CspCI CAANNNNNGTGG 4 cut(s) 696, 731, 1161, 1196
CviAII CATG 7 cut(s) 205, 407, 540, 622, 840, 1154, 1178
CviQI GTAC 7 cut(s) 75, 336, 463, 479, 518, 807, 1459
DdeI CTNAG 6 cut(s) 296, 448, 667, 954, 1027, 1527
DpnI GATC 4 cut(s) 324, 420, 681, 1506
DpnII GATC 4 cut(s) 322, 418, 679, 1504
DraI TTTAAA 1 cut(s) 1422
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 368, 1294
EarI CTCTTC 2 cut(s) 368, 1294
Eco130I CCWWGG 4 cut(s) 222, 326, 608, 621
Eco47I GGWCC 3 cut(s) 732, 1004, 1415
Eco72I CACGTG 1 cut(s) 743
Eco81I CCTNAGG 1 cut(s) 1027
EcoRII CCWGG 2 cut(s) 1012, 1377
EcoT14I CCWWGG 4 cut(s) 222, 326, 608, 621
EcoT22I ATGCAT 2 cut(s) 412, 1155
ErhI CCWWGG 4 cut(s) 222, 326, 608, 621
Esp3I CGTCTC 1 cut(s) 1245
FaeI CATG 7 cut(s) 208, 410, 543, 625, 843, 1157, 1181
FaqI GGGAC 1 cut(s) 449
FatI CATG 7 cut(s) 204, 406, 539, 621, 839, 1153, 1177
FauI CCCGC 1 cut(s) 286
FbaI TGATCA 1 cut(s) 418
Fnu4HI GCNGC 6 cut(s) 84, 87, 140, 1200, 1437, 1440
FokI GGATG 4 cut(s) 208, 789, 971, 996
Fsp4HI GCNGC 6 cut(s) 84, 87, 140, 1200, 1437, 1440
GlaI GCGC 2 cut(s) 138, 379
GluI GCNGC 6 cut(s) 84, 87, 140, 1200, 1437, 1440
GsaI CCCAGC 1 cut(s) 864
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 2 cut(s) 129, 865
HapII CCGG 2 cut(s) 120, 774
HgaI GACGC 3 cut(s) 89, 1158, 1262
HhaI GCGC 2 cut(s) 139, 380
Hin1II CATG 7 cut(s) 208, 410, 543, 625, 843, 1157, 1181
Hin6I GCGC 2 cut(s) 137, 378
HinP1I GCGC 2 cut(s) 137, 378
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HindIII AAGCTT 2 cut(s) 53, 674
HinfI GANTC 2 cut(s) 488, 1272
HpaII CCGG 2 cut(s) 120, 774
HphI GGTGA 3 cut(s) 899, 1205, 1246
Hpy166II GTNNAC 6 cut(s) 75, 240, 591, 1007, 1056, 1213
Hpy188I TCNGA 3 cut(s) 20, 1123, 1282
Hpy188III TCNNGA 8 cut(s) 304, 562, 582, 645, 989, 1029, 1269, 1390
Hpy8I GTNNAC 6 cut(s) 75, 240, 591, 1007, 1056, 1213
HpyAV CCTTC 2 cut(s) 588, 1387
HpyCH4III ACNGT 7 cut(s) 340, 527, 698, 811, 941, 1210, 1458
HpyCH4IV ACGT 1 cut(s) 742
HpyF3I CTNAG 6 cut(s) 296, 448, 667, 954, 1027, 1527
HpySE526I ACGT 1 cut(s) 742
Hsp92II CATG 7 cut(s) 208, 410, 543, 625, 843, 1157, 1181
HspAI GCGC 2 cut(s) 137, 378
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 4 cut(s) 322, 418, 679, 1504
LmnI GCTCC 3 cut(s) 107, 722, 1451
Lsp1109I GCAGC 6 cut(s) 95, 98, 151, 1211, 1426, 1448
LweI GCATC 5 cut(s) 309, 457, 1140, 1186, 1505
MaeII ACGT 1 cut(s) 742
MaeIII GTNAC 5 cut(s) 340, 349, 738, 782, 1360
MalI GATC 4 cut(s) 324, 420, 681, 1506
MbiI CCGCTC 1 cut(s) 1345
MboI GATC 4 cut(s) 322, 418, 679, 1504
MboII GAAGA 6 cut(s) 33, 323, 355, 577, 1311, 1339
MfeI CAATTG 1 cut(s) 867
MhlI GDGCHC 2 cut(s) 115, 1456
MluI ACGCGT 2 cut(s) 1167, 1254
MmeI TCCRAC 2 cut(s) 166, 700
MnlI CCTC 9 cut(s) 182, 185, 949, 1025, 1031, 1036, 1276, 1306, 1470
Mph1103I ATGCAT 2 cut(s) 412, 1155
MroXI GAANNNNTTC 1 cut(s) 1276
MseI TTAA 4 cut(s) 765, 947, 1421, 1473
MslI CAYNNNNRTG 2 cut(s) 626, 1367
MspA1I CMGCKG 1 cut(s) 1439
MspCI CTTAAG 1 cut(s) 1472
MspI CCGG 2 cut(s) 120, 774
MspR9I CCNGG 3 cut(s) 120, 1014, 1379
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 2 cut(s) 799, 1442
MvaI CCWGG 2 cut(s) 1014, 1379
MvnI CGCG 3 cut(s) 1064, 1169, 1256
NciI CCSGG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 621
NdeII GATC 4 cut(s) 322, 418, 679, 1504
NlaIII CATG 7 cut(s) 208, 410, 543, 625, 843, 1157, 1181
NlaIV GGNNCC 4 cut(s) 112, 733, 1095, 1388
NmeAIII GCCGAG 1 cut(s) 1336
NmuCI GTSAC 3 cut(s) 349, 738, 1360
NsiI ATGCAT 2 cut(s) 412, 1155
NspI RCATGY 3 cut(s) 410, 1157, 1181
NspV TTCGAA 1 cut(s) 152
PaeI GCATGC 1 cut(s) 1157
PctI GAATGC 2 cut(s) 799, 1442
PdmI GAANNNNTTC 1 cut(s) 1276
PfeI GAWTC 2 cut(s) 488, 1272
PflMI CCANNNNNTGG 1 cut(s) 1013
PfoI TCCNGGA 1 cut(s) 1377
PkrI GCNGC 6 cut(s) 85, 88, 141, 1201, 1438, 1441
PmaCI CACGTG 1 cut(s) 743
PmlI CACGTG 1 cut(s) 743
Ppu21I YACGTR 1 cut(s) 743
Psp6I CCWGG 2 cut(s) 1012, 1377
PspCI CACGTG 1 cut(s) 743
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 2 cut(s) 1012, 1377
PspN4I GGNNCC 4 cut(s) 112, 733, 1095, 1388
PspPI GGNCC 4 cut(s) 732, 863, 1004, 1415
PvuII CAGCTG 1 cut(s) 1439
RsaI GTAC 7 cut(s) 76, 337, 464, 480, 519, 808, 1460
RsaNI GTAC 7 cut(s) 75, 336, 463, 479, 518, 807, 1459
RseI CAYNNNNRTG 2 cut(s) 626, 1367
SaqAI TTAA 4 cut(s) 765, 947, 1421, 1473
SatI GCNGC 6 cut(s) 84, 87, 140, 1200, 1437, 1440
Sau3AI GATC 4 cut(s) 322, 418, 679, 1504
Sau96I GGNCC 4 cut(s) 732, 863, 1004, 1415
ScaI AGTACT 2 cut(s) 519, 1460
ScrFI CCNGG 3 cut(s) 120, 1014, 1379
SduI GDGCHC 2 cut(s) 115, 1456
SfaNI GCATC 5 cut(s) 309, 457, 1140, 1186, 1505
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 3 cut(s) 732, 1004, 1415
SmiMI CAYNNNNRTG 2 cut(s) 626, 1367
SmlI CTYRAG 1 cut(s) 1472
SmoI CTYRAG 1 cut(s) 1472
SphI GCATGC 1 cut(s) 1157
SsiI CCGC 2 cut(s) 279, 1345
SspI AATATT 1 cut(s) 904
StyD4I CCNGG 3 cut(s) 118, 1012, 1377
StyI CCWWGG 4 cut(s) 222, 326, 608, 621
TaaI ACNGT 7 cut(s) 340, 527, 698, 811, 941, 1210, 1458
TaiI ACGT 1 cut(s) 745
TaqI TCGA 1 cut(s) 152
TaqII GACCGA 1 cut(s) 1403
TatI WGTACW 5 cut(s) 335, 478, 517, 806, 1458
TfiI GAWTC 2 cut(s) 488, 1272
Tru1I TTAA 4 cut(s) 765, 947, 1421, 1473
Tru9I TTAA 4 cut(s) 765, 947, 1421, 1473
TscAI CASTG 1 cut(s) 1299
TseFI GTSAC 3 cut(s) 349, 738, 1360
TseI GCWGC 6 cut(s) 83, 86, 139, 1199, 1436, 1439
Tsp45I GTSAC 3 cut(s) 349, 738, 1360
TspDTI ATGAA 4 cut(s) 17, 932, 975, 1479
TspGWI ACGGA 3 cut(s) 92, 156, 1393
TspRI CASTG 1 cut(s) 1299
Van91I CCANNNNNTGG 1 cut(s) 1013
Vha464I CTTAAG 1 cut(s) 1472
VpaK11BI GGWCC 3 cut(s) 732, 1004, 1415
XapI RAATTY 3 cut(s) 962, 1066, 1237
XceI RCATGY 3 cut(s) 410, 1157, 1181
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 1276
ZrmI AGTACT 2 cut(s) 519, 1460
Zsp2I ATGCAT 2 cut(s) 412, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.