Rorug07G0029300

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0011500
Physical Location & Seq
Reverse (-)
2041922 .. 2045635
3714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0029300.1

Sequence Viewer

Length: 1356 bp
ATGGTGTCCTCCTCTCAGAACAATAACAAAACCCTCCATTCCCTCCTTGCCACAAAGCTCACCAAGCTCACAATCCCACCCTCCTCCTCTTCTCCGCCTCAAGACTTCGACTTTAACGACGTCTTCGGCCCCCCGACCGCCTCCAACGACGGCGGCGCGTCCACGTCATACTCGCCTTCGTTACTCGTCATCCACAACCGCTCCCACTCCTTCGTCGGCCCCTCCCCGCGCTTCACTCCCTCCTCCTCCTCCTCCTCCCTTCTCCCCTTCACCGCCGCCGACTCCGACTCCGAGGACGACGCCGTTTCCGAAACCCTGAAAGATGAGGAATCGGCGGTCCGGAAGTTCGGGCCTTGGGATTTCGAGATTCTCAGAGTCGTCGGCAAGGGCGCGTTCGGGAAGGTGTTTCAGGTGAGGAAGAAGAGCAGTGATGGCGATGAAGATGGTGATGGGCTTTATGCCATGAAGGTCATGAGGAAGGAGACTGTTATCAAGAAGAACCATGTGGATTACATGAAGGCTGAGAGGGATATCCTCACCAAAGTTGTGCACCCTTTTATTGTTCAGCTCCGCTACTCTTTTCAGACCAAGTGTAAGCTGTACTTGATCATGGATTTCATGAATGGAGGGCATTTGTTCTATCATTTGTACCGGCAGGGGATATTCAGTGAGGATCAGGCGAGGTTTTATACTGCTGAGATAGTGTCTGCTGTTTCACATCTTCACAAATGCGGGATTGTGCATCGGGACCTTAAGCCGGAAAATGTTCTCATGGATGCTGATGGGCATGTGATGCTGACGGATTTTGGACTAGCAAAGGAAATTGATGAAGATAGCAGATCGAATTCAATGTGTGGAACCACCGAATACATGGCTCCTGAAATTCTGCTGTCTAAAGGCCACAACAAAGATGCTGATTGGTGGAGCATTGGTATACTTTTGTATGAGATGCTAAGTGGTCAGCCACCATTTACGCATGCAAATAGAAAGAAACTTCAGGAGAGAATCATCAGAGAGAAAGTTAAACTTCCACCTTACCTTACCACTGAAGCTCACTCTTTGCTCAAAGGATTGCTACAAAAGGAACCATCCCAAAGGTTAGGCAGTGGCCCCAGTGGAGGTGATAATGTCAAAAGTCATAAATGGTTTAAAACAATCAACTGGAAGAAGTTGGAGGCCAGAGAACTGCTCCCAAAGTTCAAGCCAGATGTGACTGGAAAAGAATGCACGGCCAATTTTGACCGGTGCTGGACAACAATGCCTCTAGATGACTCACCAGCTCCTACACCAACAGCGGGTGAGCATTTTCAGGGATATACTTATGTGGCACCTAACACTTGGCTTTCATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

451

Amino Acids

50.48

Weight (kDa)

6.97

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 121 - 383 1.2e-73 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 122 - 366 1.9e-39 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 201 - 282 2.7e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 225 - 365 9.6e-10 Kinase-like
Pkinase_fungal PF17667 234 - 306 2.1e-08 Fungal protein kinase
Pkinase_C PF00433 404 - 441 1.7e-06 Protein kinase C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 1327
AccBSI CCGCTC 1 cut(s) 201
AccI GTMKAC 1 cut(s) 934
AccII CGCG 3 cut(s) 158, 229, 392
AccIII TCCGGA 1 cut(s) 339
AclWI GGATC 1 cut(s) 681
AcoI YGGCCR 1 cut(s) 1230
AcsI RAATTY 2 cut(s) 844, 882
AcuI CTGAAG 2 cut(s) 980, 1068
AcyI GRCGYC 2 cut(s) 120, 300
AfaI GTAC 2 cut(s) 602, 650
AfiI CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
AflII CTTAAG 1 cut(s) 752
AgeI ACCGGT 1 cut(s) 1242
AgsI TTSAA 2 cut(s) 849, 1201
AjiI CACGTC 1 cut(s) 165
AluBI AGCT 6 cut(s) 58, 67, 568, 598, 1052, 1280
AluI AGCT 6 cut(s) 58, 67, 568, 598, 1052, 1280
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 1 cut(s) 476
Alw44I GTGCAC 1 cut(s) 548
AlwI GGATC 1 cut(s) 681
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 7 cut(s) 127, 217, 350, 898, 1108, 1176, 1230
ApaLI GTGCAC 1 cut(s) 548
ApoI RAATTY 2 cut(s) 844, 882
AsiGI ACCGGT 1 cut(s) 1242
Asp700I GAANNNNTTC 1 cut(s) 765
AspLEI GCGC 3 cut(s) 158, 231, 392
AspS9I GGNCC 6 cut(s) 128, 218, 337, 350, 748, 1109
AsuHPI GGTGA 8 cut(s) 52, 262, 424, 458, 529, 1133, 1266, 1310
AvaII GGWCC 2 cut(s) 337, 748
BaeGI GKGCMC 1 cut(s) 552
BanI GGYRCC 1 cut(s) 1327
BbsI GAAGAC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 552
BccI CCATC 5 cut(s) 425, 437, 443, 776, 1096
BceAI ACGGC 3 cut(s) 166, 287, 1245
BclI TGATCA 1 cut(s) 606
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 2 cut(s) 812, 1265
BfrI CTTAAG 1 cut(s) 752
BisI GCNGC 2 cut(s) 154, 276
BlsI GCNGC 2 cut(s) 155, 277
Bme18I GGWCC 2 cut(s) 337, 748
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 6 cut(s) 128, 218, 337, 350, 748, 1109
BmiI GGNNCC 8 cut(s) 130, 220, 749, 859, 876, 1086, 1111, 1329
BmrI ACTGGG 1 cut(s) 1107
BmsI GCATC 5 cut(s) 751, 766, 783, 901, 939
BmuI ACTGGG 1 cut(s) 1107
BpiI GAAGAC 1 cut(s) 115
BplI GAGNNNNNCTC 2 cut(s) 1173, 1205
BpuEI CTTGAG 1 cut(s) 84
BsaHI GRCGYC 2 cut(s) 120, 300
BsaJI CCNNGG 2 cut(s) 291, 353
BsaWI WCCGGW 2 cut(s) 339, 1242
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 272, 302, 916, 946
Bsc4I CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
Bse118I RCCGGY 2 cut(s) 651, 1242
Bse1I ACTGG 3 cut(s) 1113, 1166, 1219
BseAI TCCGGA 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 291, 353
BseGI GGATG 3 cut(s) 189, 781, 1088
BseLI CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
BseMII CTCAG 4 cut(s) 29, 385, 513, 687
BseNI ACTGG 3 cut(s) 1113, 1166, 1219
BseRI GAGGAG 7 cut(s) 73, 76, 232, 235, 238, 241, 244
BseSI GKGCMC 1 cut(s) 552
Bsh1236I CGCG 3 cut(s) 158, 229, 392
Bsh1285I CGRYCG 1 cut(s) 138
BshFI GGCC 7 cut(s) 129, 219, 352, 900, 1110, 1178, 1232
BshNI GGYRCC 1 cut(s) 1327
BshTI ACCGGT 1 cut(s) 1242
BsiEI CGRYCG 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 4 cut(s) 340, 652, 758, 1243
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 3 cut(s) 757, 1118, 1295
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 761
BsmI GAATGC 1 cut(s) 1229
BsnI GGCC 7 cut(s) 129, 219, 352, 900, 1110, 1178, 1232
Bsp1286I GDGCHC 1 cut(s) 552
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 3 cut(s) 606, 673, 839
BspANI GGCC 7 cut(s) 129, 219, 352, 900, 1110, 1178, 1232
BspCNI CTCAG 4 cut(s) 28, 384, 514, 688
BspEI TCCGGA 1 cut(s) 339
BspFNI CGCG 3 cut(s) 158, 229, 392
BspHI TCATGA 2 cut(s) 471, 618
BspLI GGNNCC 8 cut(s) 130, 220, 749, 859, 876, 1086, 1111, 1329
BspPI GGATC 1 cut(s) 681
BspQI GCTCTTC 1 cut(s) 416
BspT107I GGYRCC 1 cut(s) 1327
BspTI CTTAAG 1 cut(s) 752
BsrBI CCGCTC 1 cut(s) 201
BsrFI RCCGGY 2 cut(s) 651, 1242
BsrI ACTGG 3 cut(s) 1113, 1166, 1219
BssAI RCCGGY 2 cut(s) 651, 1242
BssECI CCNNGG 2 cut(s) 291, 353
BssMI GATC 3 cut(s) 606, 673, 839
BssNAI GTATAC 1 cut(s) 935
BssNI GRCGYC 2 cut(s) 120, 300
BssT1I CCWWGG 1 cut(s) 353
Bst1107I GTATAC 1 cut(s) 935
Bst4CI ACNGT 1 cut(s) 487
Bst6I CTCTTC 2 cut(s) 94, 416
BstACI GRCGYC 2 cut(s) 120, 300
BstAFI CTTAAG 1 cut(s) 752
BstAPI GCANNNNNTGC 1 cut(s) 793
BstC8I GCNNGC 1 cut(s) 978
BstDEI CTNAG 5 cut(s) 15, 371, 522, 696, 953
BstF5I GGATG 3 cut(s) 189, 781, 1088
BstFNI CGCG 3 cut(s) 158, 229, 392
BstHHI GCGC 3 cut(s) 158, 231, 392
BstKTI GATC 3 cut(s) 609, 676, 842
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 3 cut(s) 606, 673, 839
BstMCI CGRYCG 1 cut(s) 138
BstMWI GCNNNNNNNGC 3 cut(s) 64, 432, 793
BstNSI RCATGY 2 cut(s) 791, 980
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 3 cut(s) 158, 229, 392
BstV2I GAAGAC 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 935
BsuRI GGCC 7 cut(s) 129, 219, 352, 900, 1110, 1178, 1232
BtgZI GCGATG 1 cut(s) 450
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 3 cut(s) 189, 781, 1088
BtsI GCAGTG 2 cut(s) 433, 1111
BtsIMutI CAGTG 5 cut(s) 433, 673, 1044, 1111, 1120
Cac8I GCNNGC 1 cut(s) 978
CciI TCATGA 2 cut(s) 471, 618
CfoI GCGC 3 cut(s) 158, 231, 392
Cfr10I RCCGGY 2 cut(s) 651, 1242
Cfr13I GGNCC 6 cut(s) 128, 218, 337, 350, 748, 1109
CpoI CGGWCCG 1 cut(s) 337
CseI GACGC 2 cut(s) 147, 308
Csp6I GTAC 2 cut(s) 601, 649
CspAI ACCGGT 1 cut(s) 1242
CspI CGGWCCG 1 cut(s) 337
CviQI GTAC 2 cut(s) 601, 649
DdeI CTNAG 5 cut(s) 15, 371, 522, 696, 953
DpnI GATC 3 cut(s) 608, 675, 841
DpnII GATC 3 cut(s) 606, 673, 839
DraI TTTAAA 1 cut(s) 1150
EaeI YGGCCR 1 cut(s) 1230
Eam1104I CTCTTC 2 cut(s) 94, 416
EarI CTCTTC 2 cut(s) 94, 416
EciI GGCGGA 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 353
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 337, 748
Eco57I CTGAAG 2 cut(s) 980, 1068
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 844
EcoRV GATATC 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 353
FalI AAGNNNNNCTT 4 cut(s) 587, 619, 1011, 1043
FaqI GGGAC 1 cut(s) 761
FauI CCCGC 3 cut(s) 234, 725, 1288
FbaI TGATCA 1 cut(s) 606
FblI GTMKAC 1 cut(s) 934
Fnu4HI GCNGC 2 cut(s) 154, 276
FokI GGATG 3 cut(s) 176, 788, 1075
Fsp4HI GCNGC 2 cut(s) 154, 276
FspBI CTAG 2 cut(s) 812, 1265
GlaI GCGC 3 cut(s) 157, 230, 391
GluI GCNGC 2 cut(s) 154, 276
HaeIII GGCC 7 cut(s) 129, 219, 352, 900, 1110, 1178, 1232
HapII CCGG 4 cut(s) 340, 652, 758, 1243
HgaI GACGC 2 cut(s) 147, 308
HhaI GCGC 3 cut(s) 158, 231, 392
Hin1I GRCGYC 2 cut(s) 120, 300
Hin6I GCGC 3 cut(s) 156, 229, 390
HinP1I GCGC 3 cut(s) 156, 229, 390
HinfI GANTC 7 cut(s) 281, 287, 329, 367, 375, 1005, 1271
HpaII CCGG 4 cut(s) 340, 652, 758, 1243
HphI GGTGA 8 cut(s) 52, 262, 424, 458, 529, 1133, 1266, 1310
Hpy166II GTNNAC 3 cut(s) 162, 550, 935
Hpy188I TCNGA 7 cut(s) 18, 286, 292, 310, 374, 585, 1013
Hpy8I GTNNAC 3 cut(s) 162, 550, 935
Hpy99I CGWCG 5 cut(s) 122, 152, 218, 302, 383
HpyAV CCTTC 8 cut(s) 186, 220, 269, 277, 394, 460, 472, 511
HpyCH4III ACNGT 1 cut(s) 487
HpyCH4IV ACGT 2 cut(s) 120, 164
HpyCH4V TGCA 4 cut(s) 550, 742, 980, 1227
HpyF10VI GCNNNNNNNGC 3 cut(s) 64, 432, 793
HpyF3I CTNAG 5 cut(s) 15, 371, 522, 696, 953
HpySE526I ACGT 2 cut(s) 120, 164
Hsp92I GRCGYC 2 cut(s) 120, 300
HspAI GCGC 3 cut(s) 156, 229, 390
Kpn2I TCCGGA 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 3 cut(s) 606, 673, 839
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 6 cut(s) 206, 573, 880, 924, 1194, 1285
LweI GCATC 5 cut(s) 751, 766, 783, 901, 939
MaeI CTAG 2 cut(s) 812, 1265
MaeII ACGT 2 cut(s) 120, 164
MaeIII GTNAC 2 cut(s) 180, 1210
MalI GATC 3 cut(s) 608, 675, 841
MbiI CCGCTC 1 cut(s) 201
MboI GATC 3 cut(s) 606, 673, 839
MboII GAAGA 9 cut(s) 81, 115, 430, 433, 452, 508, 713, 842, 1177
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 4 cut(s) 822, 844, 882, 1234
MlyI GAGTC 4 cut(s) 275, 281, 384, 1265
MmeI TCCRAC 3 cut(s) 168, 309, 1152
MroI TCCGGA 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 765
MseI TTAA 4 cut(s) 114, 753, 1023, 1149
MspA1I CMGCKG 1 cut(s) 1295
MspCI CTTAAG 1 cut(s) 752
MspI CCGG 4 cut(s) 340, 652, 758, 1243
Mva1269I GAATGC 1 cut(s) 1229
MvnI CGCG 3 cut(s) 158, 229, 392
MwoI GCNNNNNNNGC 3 cut(s) 64, 432, 793
NdeII GATC 3 cut(s) 606, 673, 839
NlaIV GGNNCC 8 cut(s) 130, 220, 749, 859, 876, 1086, 1111, 1329
NmuCI GTSAC 1 cut(s) 1210
NspI RCATGY 2 cut(s) 791, 980
PaeI GCATGC 1 cut(s) 980
PagI TCATGA 2 cut(s) 471, 618
PciSI GCTCTTC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 306
PctI GAATGC 1 cut(s) 1229
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 3 cut(s) 329, 367, 1005
PinAI ACCGGT 1 cut(s) 1242
PkrI GCNGC 2 cut(s) 155, 277
PleI GAGTC 4 cut(s) 275, 281, 383, 1265
PpsI GAGTC 4 cut(s) 275, 281, 383, 1265
PpuMI RGGWCCY 1 cut(s) 748
Psp5II RGGWCCY 1 cut(s) 748
PspN4I GGNNCC 8 cut(s) 130, 220, 749, 859, 876, 1086, 1111, 1329
PspPI GGNCC 6 cut(s) 128, 218, 337, 350, 748, 1109
PspPPI RGGWCCY 1 cut(s) 748
RsaI GTAC 2 cut(s) 602, 650
RsaNI GTAC 2 cut(s) 601, 649
Rsr2I CGGWCCG 1 cut(s) 337
RsrII CGGWCCG 1 cut(s) 337
SapI GCTCTTC 1 cut(s) 416
SaqAI TTAA 4 cut(s) 114, 753, 1023, 1149
SatI GCNGC 2 cut(s) 154, 276
Sau3AI GATC 3 cut(s) 606, 673, 839
Sau96I GGNCC 6 cut(s) 128, 218, 337, 350, 748, 1109
SchI GAGTC 4 cut(s) 275, 281, 384, 1265
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 5 cut(s) 751, 766, 783, 901, 939
SinI GGWCC 2 cut(s) 337, 748
SmlI CTYRAG 2 cut(s) 99, 752
SmoI CTYRAG 2 cut(s) 99, 752
SphI GCATGC 1 cut(s) 980
Sse9I AATT 4 cut(s) 822, 844, 882, 1234
SspMI CTAG 2 cut(s) 812, 1265
StyI CCWWGG 1 cut(s) 353
TaaI ACNGT 1 cut(s) 487
TaiI ACGT 2 cut(s) 123, 167
TaqI TCGA 3 cut(s) 108, 363, 842
TasI AATT 4 cut(s) 822, 844, 882, 1234
TatI WGTACW 1 cut(s) 600
TauI GCSGC 2 cut(s) 156, 278
TfiI GAWTC 3 cut(s) 329, 367, 1005
Tru1I TTAA 4 cut(s) 114, 753, 1023, 1149
Tru9I TTAA 4 cut(s) 114, 753, 1023, 1149
TscAI CASTG 5 cut(s) 433, 673, 1051, 1111, 1120
TseFI GTSAC 1 cut(s) 1210
Tsp45I GTSAC 1 cut(s) 1210
TspDTI ATGAA 7 cut(s) 453, 479, 530, 607, 635, 843, 1335
TspGWI ACGGA 1 cut(s) 815
TspRI CASTG 5 cut(s) 433, 673, 1051, 1111, 1120
Vha464I CTTAAG 1 cut(s) 752
VneI GTGCAC 1 cut(s) 548
VpaK11BI GGWCC 2 cut(s) 337, 748
XapI RAATTY 2 cut(s) 844, 882
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 2 cut(s) 791, 980
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XmiI GTMKAC 1 cut(s) 934
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 2 cut(s) 812, 1265
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.