Rh7BG157700

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0011500
Physical Location & Seq
Reverse (-)
11818520 .. 11823023
4504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG157700.1

Sequence Viewer

Length: 1644 bp
ATGTCGTTTTCCTCCTTTGATCAAAGCAACGCATATACCTACCCTCTTCTTCTCTCCAACGTCTCCATTGTGAAGTCTGAAGGCTCCGAACCATTGAGCTCCAGCAATTCGAAAATGAACAGCTTGATCAGAAGAAAGCTCGTTGAAAATGCATACAGATTGAAACTTTCACACAAGGGTTTCTGTACTGGTGCAGCCGCTAAAGTTTCCAGCTCCAATGGTGCCCAACCTAGCAATGATCATGTGGGGCAGCAGAAATCTTCAAATAAAAGCGAACCGTATGACATTGCTATTGTTGGAGGAGGCATGGTTGGCATGGCCCTAGCTTGTTCCTTGGCTAGAATGCCGATGACAAAGCACTTGAAGGTGGCCATCATTGATAGCAATCCTGCATTATCTAATGGACTCTCAATCAAGAAAGAAGACCTCCCTGATGCAAGGGTCAGTACAGTAACACCTGCAACCATATCTCTCTTCAAAGATATTGGCGCTTGGAAATATGTTGAACAGCATCGACATGCATATTTTGATCAAATGCAGGTTTGGGACTATACTGGCTTAGGATATACGAGATACGATGCTAGAGATGTACATAAAGAATATCTGGGGTGTGTAGTGGAGAATAAAGTGCTGCACAGTTCTCTTTTGTCATGCATGCAGGATACAGATTTCCAGAAGACTATTTACCCTTCCAGATTATCCTCAATGGCTTTAAATCCAGGCATTCTATCTATGGGGATGAACAGCACATCATCTGGGTTGAATGAGCGTAGAAACTTAGCAAAACTGGATCTAAGTGATGGCAATAGTTTATATGCAAAGTTGGTGGTTGGAGCTGATGGGTCCAAGTCACGTGTTAGGGAATTGGCAGGATTTAAGACGACTGGATGGAAATACTCACAGAATGCAATCATTTGCACAGTAGAGCATCATGCAGAAAATCGATGTGCATGGCAAAGATTTCTACCTACCGGACCAATTGCACTTTTGCCTATTGGTGATAATTTTAGTAACATTGTTTGGACTATGAACCCGGAAGAAGCAACTGACCGTAAATCAATGGTCGAGGATGATTTTGTGAAAGCCGTAAATTCTGCTCTTGATTATGGATTTGGCCCTCATCCTAAGTCGAGCAACTTCGGAAGTGGAGGCATTTTTTCTTGGTTTAAGACAGACACAACTATTTCTGCTAATGAATACTTCAAAGTTCCGCCGAAAGTGATGAAGTTGGCATCTGAAAGAATGGTGTTTCCCTTGTCTTTGATGCATGCCAATAACTATGTGTCAAAGCATGTGGTTCTAATTGGCGATGCAGCACACACTGTTCACCCTTTGGCTGGGCAAGGAGTTAATCTGGGTTTTGGAGATGCATTTGCTCTTTCAAGAATCATTTCTGAGGGCATTGCAGTGGGAACAGATATTTCTGAGGTCAATTTGTTGAAGAAATATGAAGCAGAGAGAAAAACGGCTAATGTTACAATGATGGCAATCCTAGATGGTTTCCAGAGGGCTTACTCAGTTGATTTTGGACCTTTAAATGTTCTACGCGCTGCTGCATTCCACGGAGCACAGTACATTCCACCCCTTAAAAGGAGTATAATCTCATATGCATCAGGGGATCAGAGATTGCCAATATTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

547

Amino Acids

59.86

Weight (kDa)

8.93

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 95 - 367 1.8e-08 FAD binding domain
FAD_binding_3 PF01494 415 - 490 1.3e-11 FAD binding domain
SE PF08491 417 - 521 1.3e-07 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 466
Acc36I ACCTGC 2 cut(s) 466, 529
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 1 cut(s) 1548
AciI CCGC 2 cut(s) 198, 1211
AclWI GGATC 2 cut(s) 798, 1626
AcoI YGGCCR 1 cut(s) 369
AcsI RAATTY 1 cut(s) 1090
AcuI CTGAAG 1 cut(s) 99
AcvI CACGTG 1 cut(s) 854
AfaI GTAC 4 cut(s) 187, 448, 591, 1574
AfiI CCNNNNNNNGG 2 cut(s) 1337, 1590
AflIII ACRYGT 1 cut(s) 853
AjnI CCWGG 1 cut(s) 718
AluBI AGCT 6 cut(s) 99, 123, 139, 213, 326, 836
AluI AGCT 6 cut(s) 99, 123, 139, 213, 326, 836
Alw21I GWGCWC 2 cut(s) 101, 1570
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 798, 1626
AoxI GGCC 3 cut(s) 318, 369, 1114
ApeKI GCWGC 6 cut(s) 194, 250, 631, 1313, 1550, 1553
ApoI RAATTY 1 cut(s) 1090
Asp700I GAANNNNTTC 1 cut(s) 1390
AspLEI GCGC 2 cut(s) 491, 1550
AspS9I GGNCC 5 cut(s) 319, 843, 974, 1115, 1529
AsuC2I CCSGG 1 cut(s) 1034
AsuHPI GGTGA 2 cut(s) 1010, 1319
AsuII TTCGAA 1 cut(s) 110
AvaII GGWCC 3 cut(s) 843, 974, 1529
BaeGI GKGCMC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 371
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 101
BarI GAAGNNNNNNTAC 2 cut(s) 668, 700
BbrPI CACGTG 1 cut(s) 854
BbsI GAAGAC 2 cut(s) 429, 683
Bbv12I GWGCWC 2 cut(s) 101, 1570
BbvI GCAGC 6 cut(s) 206, 262, 618, 1325, 1537, 1540
BccI CCATC 6 cut(s) 380, 794, 833, 882, 1477, 1490
BceAI ACGGC 2 cut(s) 1070, 1482
BciT130I CCWGG 1 cut(s) 720
BciVI GTATCC 1 cut(s) 655
BclI TGATCA 4 cut(s) 19, 126, 238, 529
BcnI CCSGG 1 cut(s) 1034
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 5 cut(s) 231, 323, 339, 582, 1493
BfoI RGCGCY 1 cut(s) 492
BfuAI ACCTGC 2 cut(s) 466, 529
BfuI GTATCC 1 cut(s) 655
BisI GCNGC 7 cut(s) 195, 198, 251, 632, 1314, 1551, 1554
BlsI GCNGC 7 cut(s) 196, 199, 252, 633, 1315, 1552, 1555
Bme1390I CCNGG 2 cut(s) 720, 1034
Bme18I GGWCC 3 cut(s) 843, 974, 1529
BmgT120I GGNCC 5 cut(s) 319, 843, 974, 1115, 1529
BmiI GGNNCC 3 cut(s) 85, 223, 844
BmrFI CCNGG 2 cut(s) 720, 1034
BmsI GCATC 9 cut(s) 424, 520, 568, 937, 1241, 1254, 1300, 1357, 1619
BpiI GAAGAC 2 cut(s) 429, 683
BpmI CTGGAG 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 559
Bpu14I TTCGAA 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 1034
Bsa29I ATCGAT 1 cut(s) 943
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 2 cut(s) 384, 1487
BsaJI CCNNGG 2 cut(s) 333, 1561
BsaWI WCCGGW 1 cut(s) 971
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 2 cut(s) 1337, 1590
Bse1I ACTGG 4 cut(s) 193, 559, 792, 889
Bse3DI GCAATG 3 cut(s) 241, 285, 1401
Bse8I GATNNNNATC 2 cut(s) 384, 1487
BseBI CCWGG 1 cut(s) 720
BseCI ATCGAT 1 cut(s) 943
BseDI CCNNGG 2 cut(s) 333, 1561
BseGI GGATG 4 cut(s) 744, 893, 1075, 1120
BseJI GATNNNNATC 2 cut(s) 384, 1487
BseLI CCNNNNNNNGG 2 cut(s) 1337, 1590
BseMI GCAATG 3 cut(s) 241, 285, 1401
BseMII CTCAG 3 cut(s) 1386, 1416, 1530
BseNI ACTGG 4 cut(s) 193, 559, 792, 889
BseRI GAGGAG 1 cut(s) 315
BseSI GKGCMC 1 cut(s) 226
BseXI GCAGC 6 cut(s) 206, 262, 618, 1325, 1537, 1540
BseYI CCCAGC 1 cut(s) 1337
BsgI GTGCAG 2 cut(s) 213, 617
Bsh1236I CGCG 1 cut(s) 1548
BshFI GGCC 3 cut(s) 320, 371, 1116
BshNI GGYRCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 943
BsiHKAI GWGCWC 2 cut(s) 101, 1570
BsiSI CCGG 2 cut(s) 972, 1034
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 2 cut(s) 1337, 1590
BsmAI GTCTC 1 cut(s) 67
BsmBI CGTCTC 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 560
BsmI GAATGC 4 cut(s) 348, 723, 910, 1556
BsnI GGCC 3 cut(s) 320, 371, 1116
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 3 cut(s) 101, 226, 1570
Bsp1407I TGTACA 1 cut(s) 589
Bsp143I GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
BspACI CCGC 2 cut(s) 198, 1211
BspANI GGCC 3 cut(s) 320, 371, 1116
BspCNI CTCAG 3 cut(s) 1387, 1417, 1529
BspDI ATCGAT 1 cut(s) 943
BspFNI CGCG 1 cut(s) 1548
BspLI GGNNCC 3 cut(s) 85, 223, 844
BspMI ACCTGC 2 cut(s) 466, 529
BspPI GGATC 2 cut(s) 798, 1626
BspT104I TTCGAA 1 cut(s) 110
BspT107I GGYRCC 1 cut(s) 221
BsrDI GCAATG 3 cut(s) 241, 285, 1401
BsrGI TGTACA 1 cut(s) 589
BsrI ACTGG 4 cut(s) 193, 559, 792, 889
BssECI CCNNGG 2 cut(s) 333, 1561
BssMI GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
BssT1I CCWWGG 1 cut(s) 333
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 7 cut(s) 279, 451, 638, 922, 1052, 1324, 1572
Bst6I CTCTTC 2 cut(s) 51, 479
BstAUI TGTACA 1 cut(s) 589
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 656, 1269
BstDEI CTNAG 7 cut(s) 559, 778, 794, 1125, 1395, 1425, 1516
BstDSI CCRYGG 1 cut(s) 1561
BstF5I GGATG 4 cut(s) 744, 893, 1075, 1120
BstFNI CGCG 1 cut(s) 1548
BstH2I RGCGCY 1 cut(s) 492
BstHHI GCGC 2 cut(s) 491, 1550
BstKTI GATC 6 cut(s) 22, 129, 241, 532, 793, 1621
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 720
BstNSI RCATGY 4 cut(s) 521, 658, 1271, 1295
BstSCI CCNGG 2 cut(s) 718, 1032
BstSLI GKGCMC 1 cut(s) 226
BstUI CGCG 1 cut(s) 1548
BstV1I GCAGC 6 cut(s) 206, 262, 618, 1325, 1537, 1540
BstV2I GAAGAC 2 cut(s) 429, 683
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
Bsu15I ATCGAT 1 cut(s) 943
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 3 cut(s) 320, 371, 1116
BsuTUI ATCGAT 1 cut(s) 943
BtgI CCRYGG 1 cut(s) 1561
BtgZI GCGATG 1 cut(s) 1323
BtsCI GGATG 4 cut(s) 744, 893, 1075, 1120
BtsI GCAGTG 1 cut(s) 1413
BtsIMutI CAGTG 2 cut(s) 1320, 1413
BveI ACCTGC 2 cut(s) 466, 529
Cac8I GCNNGC 2 cut(s) 656, 1269
CfoI GCGC 2 cut(s) 491, 1550
Cfr13I GGNCC 5 cut(s) 319, 843, 974, 1115, 1529
ClaI ATCGAT 1 cut(s) 943
Csp6I GTAC 4 cut(s) 186, 447, 590, 1573
CspCI CAANNNNNGTGG 4 cut(s) 807, 842, 1275, 1310
CviQI GTAC 4 cut(s) 186, 447, 590, 1573
DdeI CTNAG 7 cut(s) 559, 778, 794, 1125, 1395, 1425, 1516
DpnI GATC 6 cut(s) 21, 128, 240, 531, 792, 1620
DpnII GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
DraI TTTAAA 2 cut(s) 714, 1536
EaeI YGGCCR 1 cut(s) 369
Eam1104I CTCTTC 2 cut(s) 51, 479
EarI CTCTTC 2 cut(s) 51, 479
EciI GGCGGA 1 cut(s) 1200
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 101
Eco47I GGWCC 3 cut(s) 843, 974, 1529
Eco53kI GAGCTC 1 cut(s) 99
Eco57I CTGAAG 1 cut(s) 99
Eco72I CACGTG 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 718
EcoT14I CCWWGG 1 cut(s) 333
EcoT22I ATGCAT 6 cut(s) 154, 523, 656, 1269, 1372, 1612
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 67
FaqI GGGAC 1 cut(s) 560
FauNDI CATATG 1 cut(s) 1606
FbaI TGATCA 4 cut(s) 19, 126, 238, 529
Fnu4HI GCNGC 7 cut(s) 195, 198, 251, 632, 1314, 1551, 1554
FokI GGATG 4 cut(s) 751, 900, 1082, 1107
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 7 cut(s) 195, 198, 251, 632, 1314, 1551, 1554
FspBI CTAG 5 cut(s) 231, 323, 339, 582, 1493
GlaI GCGC 2 cut(s) 490, 1549
GluI GCNGC 7 cut(s) 195, 198, 251, 632, 1314, 1551, 1554
GsaI CCCAGC 1 cut(s) 1341
GsuI CTGGAG 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 492
HaeIII GGCC 3 cut(s) 320, 371, 1116
HapII CCGG 2 cut(s) 972, 1034
HhaI GCGC 2 cut(s) 491, 1550
Hin6I GCGC 2 cut(s) 489, 1548
HinP1I GCGC 2 cut(s) 489, 1548
HinfI GANTC 2 cut(s) 405, 1386
HpaII CCGG 2 cut(s) 972, 1034
HphI GGTGA 2 cut(s) 1010, 1319
Hpy166II GTNNAC 1 cut(s) 1327
Hpy188I TCNGA 8 cut(s) 79, 88, 131, 1142, 1237, 1396, 1426, 1623
Hpy188III TCNNGA 7 cut(s) 415, 673, 693, 1100, 1383, 1504, 1641
Hpy8I GTNNAC 1 cut(s) 1327
HpyAV CCTTC 3 cut(s) 74, 358, 699
HpyCH4III ACNGT 7 cut(s) 279, 451, 638, 922, 1052, 1324, 1572
HpyCH4IV ACGT 2 cut(s) 60, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 7 cut(s) 559, 778, 794, 1125, 1395, 1425, 1516
HpySE526I ACGT 2 cut(s) 60, 853
HspAI GCGC 2 cut(s) 489, 1548
Ksp22I TGATCA 4 cut(s) 19, 126, 238, 529
Kzo9I GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
LmnI GCTCC 5 cut(s) 89, 104, 218, 833, 1565
Lsp1109I GCAGC 6 cut(s) 206, 262, 618, 1325, 1537, 1540
LweI GCATC 9 cut(s) 424, 520, 568, 937, 1241, 1254, 1300, 1357, 1619
MaeI CTAG 5 cut(s) 231, 323, 339, 582, 1493
MaeII ACGT 2 cut(s) 60, 853
MaeIII GTNAC 4 cut(s) 451, 849, 1010, 1474
MalI GATC 6 cut(s) 21, 128, 240, 531, 792, 1620
MboI GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
MboII GAAGA 9 cut(s) 38, 41, 144, 252, 434, 466, 688, 1049, 1453
MfeI CAATTG 1 cut(s) 978
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 3 cut(s) 101, 226, 1570
MlsI TGGCCA 1 cut(s) 371
MluCI AATT 7 cut(s) 106, 863, 978, 1003, 1090, 1302, 1432
MluNI TGGCCA 1 cut(s) 371
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 3 cut(s) 81, 277, 811
Mox20I TGGCCA 1 cut(s) 371
Mph1103I ATGCAT 6 cut(s) 154, 523, 656, 1269, 1372, 1612
MroXI GAANNNNTTC 1 cut(s) 1390
MscI TGGCCA 1 cut(s) 371
MseI TTAA 6 cut(s) 713, 876, 1167, 1350, 1535, 1587
MslI CAYNNNNRTG 2 cut(s) 516, 1406
Msp20I TGGCCA 1 cut(s) 371
MspI CCGG 2 cut(s) 972, 1034
MspR9I CCNGG 2 cut(s) 720, 1034
MunI CAATTG 1 cut(s) 978
Mva1269I GAATGC 4 cut(s) 348, 723, 910, 1556
MvaI CCWGG 1 cut(s) 720
MvnI CGCG 1 cut(s) 1548
MwoI GCNNNNNNNGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 1034
NdeI CATATG 1 cut(s) 1606
NdeII GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
NlaIV GGNNCC 3 cut(s) 85, 223, 844
NmuCI GTSAC 1 cut(s) 849
NsiI ATGCAT 6 cut(s) 154, 523, 656, 1269, 1372, 1612
NspI RCATGY 4 cut(s) 521, 658, 1271, 1295
NspV TTCGAA 1 cut(s) 110
PaeI GCATGC 2 cut(s) 658, 1271
PaqCI CACCTGC 1 cut(s) 466
PctI GAATGC 4 cut(s) 348, 723, 910, 1556
PdmI GAANNNNTTC 1 cut(s) 1390
PfeI GAWTC 1 cut(s) 1386
PkrI GCNGC 7 cut(s) 196, 199, 252, 633, 1315, 1552, 1555
PleI GAGTC 1 cut(s) 399
PmaCI CACGTG 1 cut(s) 854
PmlI CACGTG 1 cut(s) 854
PpsI GAGTC 1 cut(s) 399
Ppu21I YACGTR 1 cut(s) 854
Psp124BI GAGCTC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 718
PspCI CACGTG 1 cut(s) 854
PspFI CCCAGC 1 cut(s) 1337
PspGI CCWGG 1 cut(s) 718
PspN4I GGNNCC 3 cut(s) 85, 223, 844
PspPI GGNCC 5 cut(s) 319, 843, 974, 1115, 1529
PsuI RGATCY 1 cut(s) 790
RsaI GTAC 4 cut(s) 187, 448, 591, 1574
RsaNI GTAC 4 cut(s) 186, 447, 590, 1573
RseI CAYNNNNRTG 2 cut(s) 516, 1406
SacI GAGCTC 1 cut(s) 101
SaqAI TTAA 6 cut(s) 713, 876, 1167, 1350, 1535, 1587
SatI GCNGC 7 cut(s) 195, 198, 251, 632, 1314, 1551, 1554
Sau3AI GATC 6 cut(s) 19, 126, 238, 529, 790, 1618
Sau96I GGNCC 5 cut(s) 319, 843, 974, 1115, 1529
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 2 cut(s) 720, 1034
SduI GDGCHC 3 cut(s) 101, 226, 1570
SfaNI GCATC 9 cut(s) 424, 520, 568, 937, 1241, 1254, 1300, 1357, 1619
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 3 cut(s) 843, 974, 1529
SmiMI CAYNNNNRTG 2 cut(s) 516, 1406
SphI GCATGC 2 cut(s) 658, 1271
Sse9I AATT 7 cut(s) 106, 863, 978, 1003, 1090, 1302, 1432
SsiI CCGC 2 cut(s) 198, 1211
SspI AATATT 1 cut(s) 1635
SspMI CTAG 5 cut(s) 231, 323, 339, 582, 1493
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 2 cut(s) 718, 1032
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 7 cut(s) 279, 451, 638, 922, 1052, 1324, 1572
TaiI ACGT 2 cut(s) 63, 856
TaqI TCGA 5 cut(s) 110, 514, 943, 1065, 1130
TasI AATT 7 cut(s) 106, 863, 978, 1003, 1090, 1302, 1432
TatI WGTACW 4 cut(s) 185, 446, 589, 1572
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 1386
Tru1I TTAA 6 cut(s) 713, 876, 1167, 1350, 1535, 1587
Tru9I TTAA 6 cut(s) 713, 876, 1167, 1350, 1535, 1587
TscAI CASTG 2 cut(s) 1327, 1413
TseFI GTSAC 1 cut(s) 849
TseI GCWGC 6 cut(s) 194, 250, 631, 1313, 1550, 1553
Tsp45I GTSAC 1 cut(s) 849
TspDTI ATGAA 6 cut(s) 131, 755, 1043, 1209, 1238, 1464
TspGWI ACGGA 1 cut(s) 1578
TspRI CASTG 2 cut(s) 1327, 1413
VpaK11BI GGWCC 3 cut(s) 843, 974, 1529
XapI RAATTY 1 cut(s) 1090
XceI RCATGY 4 cut(s) 521, 658, 1271, 1295
XmnI GAANNNNTTC 1 cut(s) 1390
XspI CTAG 5 cut(s) 231, 323, 339, 582, 1493
Zsp2I ATGCAT 6 cut(s) 154, 523, 656, 1269, 1372, 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.