FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G24200 AT3G24200 |
| fragaria_vesca | FvH4_5g03680 FvH4_5g03680 FvH4_5g03680 |
| malus_domestica | MD06G1096300.v1.1 MD14G1116500.v1.1 |
| prunus_persica | Prupe.5G110900_v2.0.a1 |
| pyrus_communis | pycom06g09260 |
| rosa_chinensis | RchiOBHm_Chr7g0196051 |
| rosa_laevigata | RLG00000004070 |
| rosa_multiflora | Rmu_sc0001640.1_g000009 |
| rosa_roxburghii | Rroxscaffold_3G00259900 |
| rosa_rugosa | Rorug07G0029300 Rorug07G0029400 |
| rosa_samantha | Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900 |
| rosa_wichuraiana | Rw7G013330 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 358 |
| Acc36I | ACCTGC | 3 cut(s) | 358, 421, 868 |
| AccBSI | CCGCTC | 1 cut(s) | 661 |
| AccI | GTMKAC | 2 cut(s) | 458, 547 |
| AciI | CCGC | 2 cut(s) | 661, 1438 |
| AclWI | GGATC | 4 cut(s) | 320, 690, 733, 1518 |
| AcsI | RAATTY | 3 cut(s) | 56, 616, 1072 |
| AcuI | CTGAAG | 1 cut(s) | 1078 |
| AcvI | CACGTG | 1 cut(s) | 746 |
| AfaI | GTAC | 7 cut(s) | 79, 173, 340, 467, 522, 811, 1323 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1111, 1229, 1482 |
| AflIII | ACRYGT | 1 cut(s) | 745 |
| AgsI | TTSAA | 9 cut(s) | 55, 256, 370, 398, 597, 655, 1275, 1333, 1453 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1174 |
| AjiI | CACGTC | 1 cut(s) | 1069 |
| AjnI | CCWGG | 1 cut(s) | 1383 |
| AluBI | AGCT | 5 cut(s) | 9, 28, 89, 105, 679 |
| AluI | AGCT | 5 cut(s) | 9, 28, 89, 105, 679 |
| Alw21I | GWGCWC | 2 cut(s) | 1028, 1462 |
| AlwI | GGATC | 4 cut(s) | 320, 690, 733, 1518 |
| AoxI | GGCC | 2 cut(s) | 210, 866 |
| ApeKI | GCWGC | 4 cut(s) | 86, 1205, 1442, 1445 |
| ApoI | RAATTY | 3 cut(s) | 56, 616, 1072 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1282 |
| AspLEI | GCGC | 1 cut(s) | 991 |
| AspS9I | GGNCC | 5 cut(s) | 211, 735, 866, 1007, 1421 |
| AsuHPI | GGTGA | 4 cut(s) | 902, 1126, 1211, 1211 |
| AvaII | GGWCC | 3 cut(s) | 735, 1007, 1421 |
| AxyI | CCTNAGG | 2 cut(s) | 1030, 1504 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1313, 1346 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 560, 592 |
| BbrPI | CACGTG | 1 cut(s) | 746 |
| BbsI | GAAGAC | 3 cut(s) | 321, 575, 585 |
| Bbv12I | GWGCWC | 2 cut(s) | 1028, 1462 |
| BbvCI | CCTCAGC | 2 cut(s) | 957, 1317 |
| BbvI | GCAGC | 4 cut(s) | 98, 1217, 1429, 1432 |
| BccI | CCATC | 6 cut(s) | 272, 686, 725, 774, 1369, 1382 |
| BciT130I | CCWGG | 1 cut(s) | 1385 |
| BciVI | GTATCC | 1 cut(s) | 547 |
| BclI | TGATCA | 2 cut(s) | 12, 421 |
| BfaI | CTAG | 2 cut(s) | 231, 497 |
| BfmI | CTRYAG | 1 cut(s) | 813 |
| BfuAI | ACCTGC | 3 cut(s) | 358, 421, 868 |
| BfuI | GTATCC | 1 cut(s) | 547 |
| BisI | GCNGC | 4 cut(s) | 87, 1206, 1443, 1446 |
| BlsI | GCNGC | 4 cut(s) | 88, 1207, 1444, 1447 |
| BmcAI | AGTACT | 1 cut(s) | 522 |
| Bme1390I | CCNGG | 1 cut(s) | 1385 |
| Bme18I | GGWCC | 3 cut(s) | 735, 1007, 1421 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1174 |
| BmgBI | CACGTC | 1 cut(s) | 1069 |
| BmgT120I | GGNCC | 5 cut(s) | 211, 735, 866, 1007, 1421 |
| BmiI | GGNNCC | 1 cut(s) | 736 |
| BmrFI | CCNGG | 1 cut(s) | 1385 |
| BmsI | GCATC | 7 cut(s) | 73, 312, 412, 1133, 1146, 1192, 1249 |
| BpiI | GAAGAC | 3 cut(s) | 321, 575, 585 |
| Bpu10I | CCTNAGC | 3 cut(s) | 451, 957, 1317 |
| Bsa29I | ATCGAT | 2 cut(s) | 270, 835 |
| BsaAI | YACGTR | 1 cut(s) | 746 |
| BsaBI | GATNNNNATC | 2 cut(s) | 276, 1379 |
| BsaJI | CCNNGG | 3 cut(s) | 225, 329, 624 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1111, 1229, 1482 |
| Bse1I | ACTGG | 2 cut(s) | 451, 781 |
| Bse21I | CCTNAGG | 2 cut(s) | 1030, 1504 |
| Bse3DI | GCAATG | 4 cut(s) | 133, 163, 177, 1311 |
| Bse8I | GATNNNNATC | 2 cut(s) | 276, 1379 |
| BseBI | CCWGG | 1 cut(s) | 1385 |
| BseCI | ATCGAT | 2 cut(s) | 270, 835 |
| BseDI | CCNNGG | 3 cut(s) | 225, 329, 624 |
| BseGI | GGATG | 5 cut(s) | 88, 785, 967, 1012, 1393 |
| BseJI | GATNNNNATC | 2 cut(s) | 276, 1379 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1111, 1229, 1482 |
| BseMI | GCAATG | 4 cut(s) | 133, 163, 177, 1311 |
| BseMII | CTCAG | 5 cut(s) | 677, 948, 1278, 1308, 1518 |
| BseNI | ACTGG | 2 cut(s) | 451, 781 |
| BseXI | GCAGC | 4 cut(s) | 98, 1217, 1429, 1432 |
| BseYI | CCCAGC | 2 cut(s) | 863, 1229 |
| BsgI | GTGCAG | 1 cut(s) | 509 |
| BshFI | GGCC | 2 cut(s) | 212, 868 |
| BshVI | ATCGAT | 2 cut(s) | 270, 835 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1028, 1462 |
| BsiWI | CGTACG | 1 cut(s) | 171 |
| BslFI | GGGAC | 2 cut(s) | 309, 452 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1111, 1229, 1482 |
| BsmFI | GGGAC | 2 cut(s) | 309, 452 |
| BsmI | GAATGC | 3 cut(s) | 240, 802, 1448 |
| BsnI | GGCC | 2 cut(s) | 212, 868 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1028, 1462 |
| Bsp143I | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| Bsp19I | CCATGG | 1 cut(s) | 624 |
| BspACI | CCGC | 2 cut(s) | 661, 1438 |
| BspANI | GGCC | 2 cut(s) | 212, 868 |
| BspCNI | CTCAG | 5 cut(s) | 678, 949, 1279, 1309, 1517 |
| BspDI | ATCGAT | 2 cut(s) | 270, 835 |
| BspLI | GGNNCC | 1 cut(s) | 736 |
| BspMI | ACCTGC | 3 cut(s) | 358, 421, 868 |
| BspPI | GGATC | 4 cut(s) | 320, 690, 733, 1518 |
| BsrBI | CCGCTC | 1 cut(s) | 661 |
| BsrDI | GCAATG | 4 cut(s) | 133, 163, 177, 1311 |
| BsrI | ACTGG | 2 cut(s) | 451, 781 |
| BssECI | CCNNGG | 3 cut(s) | 225, 329, 624 |
| BssMI | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| BssNAI | GTATAC | 2 cut(s) | 459, 548 |
| BssT1I | CCWWGG | 3 cut(s) | 225, 329, 624 |
| Bst1107I | GTATAC | 2 cut(s) | 459, 548 |
| Bst2UI | CCWGG | 1 cut(s) | 1385 |
| Bst4CI | ACNGT | 6 cut(s) | 343, 701, 814, 1216, 1326, 1464 |
| Bst6I | CTCTTC | 1 cut(s) | 371 |
| BstBAI | YACGTR | 1 cut(s) | 746 |
| BstC8I | GCNNGC | 3 cut(s) | 30, 1161, 1440 |
| BstDEI | CTNAG | 9 cut(s) | 451, 670, 686, 957, 1017, 1030, 1287, 1317, 1504 |
| BstDSI | CCRYGG | 1 cut(s) | 624 |
| BstF5I | GGATG | 5 cut(s) | 88, 785, 967, 1012, 1393 |
| BstHHI | GCGC | 1 cut(s) | 991 |
| BstKTI | GATC | 7 cut(s) | 15, 328, 424, 685, 728, 943, 1513 |
| BstMBI | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 204 |
| BstNI | CCWGG | 1 cut(s) | 1385 |
| BstNSI | RCATGY | 3 cut(s) | 413, 1163, 1187 |
| BstSCI | CCNGG | 1 cut(s) | 1383 |
| BstSFI | CTRYAG | 1 cut(s) | 813 |
| BstV1I | GCAGC | 4 cut(s) | 98, 1217, 1429, 1432 |
| BstV2I | GAAGAC | 3 cut(s) | 321, 575, 585 |
| BstX2I | RGATCY | 2 cut(s) | 682, 725 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 631 |
| BstYI | RGATCY | 2 cut(s) | 682, 725 |
| BstZ17I | GTATAC | 2 cut(s) | 459, 548 |
| Bsu15I | ATCGAT | 2 cut(s) | 270, 835 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1030, 1504 |
| BsuI | GTATCC | 1 cut(s) | 547 |
| BsuRI | GGCC | 2 cut(s) | 212, 868 |
| BsuTUI | ATCGAT | 2 cut(s) | 270, 835 |
| BtgI | CCRYGG | 1 cut(s) | 624 |
| BtrI | CACGTC | 1 cut(s) | 1069 |
| BtsCI | GGATG | 5 cut(s) | 88, 785, 967, 1012, 1393 |
| BtsI | GCAGTG | 1 cut(s) | 1305 |
| BtsIMutI | CAGTG | 3 cut(s) | 239, 1212, 1305 |
| BveI | ACCTGC | 3 cut(s) | 358, 421, 868 |
| Cac8I | GCNNGC | 3 cut(s) | 30, 1161, 1440 |
| CfoI | GCGC | 1 cut(s) | 991 |
| Cfr13I | GGNCC | 5 cut(s) | 211, 735, 866, 1007, 1421 |
| ClaI | ATCGAT | 2 cut(s) | 270, 835 |
| CseI | GACGC | 1 cut(s) | 1164 |
| Csp6I | GTAC | 7 cut(s) | 78, 172, 339, 466, 521, 810, 1322 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 699, 734, 1167, 1202 |
| CviAII | CATG | 9 cut(s) | 134, 199, 208, 410, 543, 625, 843, 1160, 1184 |
| CviQI | GTAC | 7 cut(s) | 78, 172, 339, 466, 521, 810, 1322 |
| DdeI | CTNAG | 9 cut(s) | 451, 670, 686, 957, 1017, 1030, 1287, 1317, 1504 |
| DpnI | GATC | 7 cut(s) | 14, 327, 423, 684, 727, 942, 1512 |
| DpnII | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| DraI | TTTAAA | 1 cut(s) | 1060 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1174 |
| Eam1104I | CTCTTC | 1 cut(s) | 371 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1174 |
| EarI | CTCTTC | 1 cut(s) | 371 |
| Eco130I | CCWWGG | 3 cut(s) | 225, 329, 624 |
| Eco47I | GGWCC | 3 cut(s) | 735, 1007, 1421 |
| Eco57I | CTGAAG | 1 cut(s) | 1078 |
| Eco72I | CACGTG | 1 cut(s) | 746 |
| Eco81I | CCTNAGG | 2 cut(s) | 1030, 1504 |
| EcoRII | CCWGG | 1 cut(s) | 1383 |
| EcoT14I | CCWWGG | 3 cut(s) | 225, 329, 624 |
| EcoT22I | ATGCAT | 3 cut(s) | 415, 1161, 1264 |
| ErhI | CCWWGG | 3 cut(s) | 225, 329, 624 |
| FaeI | CATG | 9 cut(s) | 137, 202, 211, 413, 546, 628, 846, 1163, 1187 |
| FaqI | GGGAC | 2 cut(s) | 309, 452 |
| FatI | CATG | 9 cut(s) | 133, 198, 207, 409, 542, 624, 842, 1159, 1183 |
| FauI | CCCGC | 2 cut(s) | 654, 1431 |
| FauNDI | CATATG | 1 cut(s) | 1498 |
| FbaI | TGATCA | 2 cut(s) | 12, 421 |
| FblI | GTMKAC | 2 cut(s) | 458, 547 |
| Fnu4HI | GCNGC | 4 cut(s) | 87, 1206, 1443, 1446 |
| FokI | GGATG | 5 cut(s) | 95, 792, 974, 999, 1400 |
| Fsp4HI | GCNGC | 4 cut(s) | 87, 1206, 1443, 1446 |
| FspBI | CTAG | 2 cut(s) | 231, 497 |
| GlaI | GCGC | 1 cut(s) | 990 |
| GluI | GCNGC | 4 cut(s) | 87, 1206, 1443, 1446 |
| GsaI | CCCAGC | 2 cut(s) | 867, 1233 |
| HaeIII | GGCC | 2 cut(s) | 212, 868 |
| HgaI | GACGC | 1 cut(s) | 1164 |
| HhaI | GCGC | 1 cut(s) | 991 |
| Hin1II | CATG | 9 cut(s) | 137, 202, 211, 413, 546, 628, 846, 1163, 1187 |
| Hin6I | GCGC | 1 cut(s) | 989 |
| HinP1I | GCGC | 1 cut(s) | 989 |
| HindIII | AAGCTT | 1 cut(s) | 26 |
| HinfI | GANTC | 3 cut(s) | 297, 1278, 1518 |
| HphI | GGTGA | 4 cut(s) | 902, 1126, 1211, 1211 |
| Hpy166II | GTNNAC | 4 cut(s) | 166, 459, 548, 1219 |
| Hpy188I | TCNGA | 7 cut(s) | 20, 687, 730, 1097, 1129, 1288, 1421 |
| Hpy188III | TCNNGA | 7 cut(s) | 307, 323, 565, 585, 1082, 1275, 1533 |
| Hpy8I | GTNNAC | 4 cut(s) | 166, 459, 548, 1219 |
| HpyAV | CCTTC | 1 cut(s) | 591 |
| HpyCH4III | ACNGT | 6 cut(s) | 343, 701, 814, 1216, 1326, 1464 |
| HpyCH4IV | ACGT | 2 cut(s) | 745, 1068 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 204 |
| HpyF3I | CTNAG | 9 cut(s) | 451, 670, 686, 957, 1017, 1030, 1287, 1317, 1504 |
| HpySE526I | ACGT | 2 cut(s) | 745, 1068 |
| Hsp92II | CATG | 9 cut(s) | 137, 202, 211, 413, 546, 628, 846, 1163, 1187 |
| HspAI | GCGC | 1 cut(s) | 989 |
| Ksp22I | TGATCA | 2 cut(s) | 12, 421 |
| Kzo9I | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| LmnI | GCTCC | 3 cut(s) | 94, 110, 1457 |
| Lsp1109I | GCAGC | 4 cut(s) | 98, 1217, 1429, 1432 |
| LweI | GCATC | 7 cut(s) | 73, 312, 412, 1133, 1146, 1192, 1249 |
| MaeI | CTAG | 2 cut(s) | 231, 497 |
| MaeII | ACGT | 2 cut(s) | 745, 1068 |
| MaeIII | GTNAC | 3 cut(s) | 343, 902, 1366 |
| MalI | GATC | 7 cut(s) | 14, 327, 423, 684, 727, 942, 1512 |
| MbiI | CCGCTC | 1 cut(s) | 661 |
| MboI | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| MboII | GAAGA | 6 cut(s) | 33, 326, 358, 580, 585, 1345 |
| MfeI | CAATTG | 1 cut(s) | 870 |
| MflI | RGATCY | 2 cut(s) | 682, 725 |
| MhlI | GDGCHC | 2 cut(s) | 1028, 1462 |
| MluCI | AATT | 9 cut(s) | 56, 529, 616, 784, 870, 895, 982, 1072, 1524 |
| MlyI | GAGTC | 1 cut(s) | 291 |
| MmeI | TCCRAC | 2 cut(s) | 169, 703 |
| Mph1103I | ATGCAT | 3 cut(s) | 415, 1161, 1264 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1282 |
| MseI | TTAA | 4 cut(s) | 768, 1059, 1242, 1479 |
| MslI | CAYNNNNRTG | 4 cut(s) | 408, 629, 1164, 1373 |
| MspR9I | CCNGG | 1 cut(s) | 1385 |
| MunI | CAATTG | 1 cut(s) | 870 |
| Mva1269I | GAATGC | 3 cut(s) | 240, 802, 1448 |
| MvaI | CCWGG | 1 cut(s) | 1385 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 204 |
| NcoI | CCATGG | 1 cut(s) | 624 |
| NdeI | CATATG | 1 cut(s) | 1498 |
| NdeII | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| NlaIII | CATG | 9 cut(s) | 137, 202, 211, 413, 546, 628, 846, 1163, 1187 |
| NlaIV | GGNNCC | 1 cut(s) | 736 |
| NmeAIII | GCCGAG | 1 cut(s) | 279 |
| NmuCI | GTSAC | 1 cut(s) | 1366 |
| NsiI | ATGCAT | 3 cut(s) | 415, 1161, 1264 |
| NspI | RCATGY | 3 cut(s) | 413, 1163, 1187 |
| PaeI | GCATGC | 1 cut(s) | 1163 |
| PaqCI | CACCTGC | 1 cut(s) | 358 |
| PctI | GAATGC | 3 cut(s) | 240, 802, 1448 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1282 |
| PfeI | GAWTC | 2 cut(s) | 1278, 1518 |
| Pfl23II | CGTACG | 1 cut(s) | 171 |
| PfoI | TCCNGGA | 1 cut(s) | 1383 |
| PkrI | GCNGC | 4 cut(s) | 88, 1207, 1444, 1447 |
| PleI | GAGTC | 1 cut(s) | 291 |
| PmaCI | CACGTG | 1 cut(s) | 746 |
| PmlI | CACGTG | 1 cut(s) | 746 |
| PpsI | GAGTC | 1 cut(s) | 291 |
| Ppu21I | YACGTR | 1 cut(s) | 746 |
| Psp6I | CCWGG | 1 cut(s) | 1383 |
| PspCI | CACGTG | 1 cut(s) | 746 |
| PspFI | CCCAGC | 2 cut(s) | 863, 1229 |
| PspGI | CCWGG | 1 cut(s) | 1383 |
| PspLI | CGTACG | 1 cut(s) | 171 |
| PspN4I | GGNNCC | 1 cut(s) | 736 |
| PspPI | GGNCC | 5 cut(s) | 211, 735, 866, 1007, 1421 |
| PsuI | RGATCY | 2 cut(s) | 682, 725 |
| RsaI | GTAC | 7 cut(s) | 79, 173, 340, 467, 522, 811, 1323 |
| RsaNI | GTAC | 7 cut(s) | 78, 172, 339, 466, 521, 810, 1322 |
| RseI | CAYNNNNRTG | 4 cut(s) | 408, 629, 1164, 1373 |
| SaqAI | TTAA | 4 cut(s) | 768, 1059, 1242, 1479 |
| SatI | GCNGC | 4 cut(s) | 87, 1206, 1443, 1446 |
| Sau3AI | GATC | 7 cut(s) | 12, 325, 421, 682, 725, 940, 1510 |
| Sau96I | GGNCC | 5 cut(s) | 211, 735, 866, 1007, 1421 |
| ScaI | AGTACT | 1 cut(s) | 522 |
| SchI | GAGTC | 1 cut(s) | 291 |
| ScrFI | CCNGG | 1 cut(s) | 1385 |
| SduI | GDGCHC | 2 cut(s) | 1028, 1462 |
| SfaNI | GCATC | 7 cut(s) | 73, 312, 412, 1133, 1146, 1192, 1249 |
| SfcI | CTRYAG | 1 cut(s) | 813 |
| SinI | GGWCC | 3 cut(s) | 735, 1007, 1421 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 408, 629, 1164, 1373 |
| SphI | GCATGC | 1 cut(s) | 1163 |
| Sse9I | AATT | 9 cut(s) | 56, 529, 616, 784, 870, 895, 982, 1072, 1524 |
| SsiI | CCGC | 2 cut(s) | 661, 1438 |
| SspMI | CTAG | 2 cut(s) | 231, 497 |
| StyD4I | CCNGG | 1 cut(s) | 1383 |
| StyI | CCWWGG | 3 cut(s) | 225, 329, 624 |
| TaaI | ACNGT | 6 cut(s) | 343, 701, 814, 1216, 1326, 1464 |
| TaiI | ACGT | 2 cut(s) | 748, 1071 |
| TaqI | TCGA | 4 cut(s) | 270, 406, 835, 1022 |
| TasI | AATT | 9 cut(s) | 56, 529, 616, 784, 870, 895, 982, 1072, 1524 |
| TatI | WGTACW | 3 cut(s) | 465, 520, 809 |
| TfiI | GAWTC | 2 cut(s) | 1278, 1518 |
| Tru1I | TTAA | 4 cut(s) | 768, 1059, 1242, 1479 |
| Tru9I | TTAA | 4 cut(s) | 768, 1059, 1242, 1479 |
| TscAI | CASTG | 3 cut(s) | 246, 1219, 1305 |
| TseFI | GTSAC | 1 cut(s) | 1366 |
| TseI | GCWGC | 4 cut(s) | 86, 1205, 1442, 1445 |
| Tsp45I | GTSAC | 1 cut(s) | 1366 |
| TspDTI | ATGAA | 5 cut(s) | 17, 174, 935, 1356, 1485 |
| TspGWI | ACGGA | 3 cut(s) | 95, 1134, 1470 |
| TspRI | CASTG | 3 cut(s) | 246, 1219, 1305 |
| VpaK11BI | GGWCC | 3 cut(s) | 735, 1007, 1421 |
| XapI | RAATTY | 3 cut(s) | 56, 616, 1072 |
| XceI | RCATGY | 3 cut(s) | 413, 1163, 1187 |
| XmiI | GTMKAC | 2 cut(s) | 458, 547 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1282 |
| XspI | CTAG | 2 cut(s) | 231, 497 |
| ZrmI | AGTACT | 1 cut(s) | 522 |
| Zsp2I | ATGCAT | 3 cut(s) | 415, 1161, 1264 |