RLG00000004070

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011500
Physical Location & Seq
Forward (+)
55850061 .. 55854333
4273 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004070

Sequence Viewer

Length: 1530 bp
ATGAACAGCTTGATCAGAAGAAAGCTCGTCGAAAATGCATACAGATTGAAACTTTCACACAAGGGTTTCTGTACTGGTGCAGCTGCTAAAGTTTCCAGCTCCAATGGTGCCCAACCAAGCAATGATCATGTGGGGCAGCAGAAATCTTCAAATAAAAGCGAACCGTATGACATTGCTATTGTTGGAGGAGGCATGGTTGGCATGGCCCTAGCTTGTTCCTTGGCTAGAATGCCGATGACAAAGCACCTGAAGGTGGCCATTATTGATAGCAATCCTGCATTATCTAATGGACTCTCAATCAAGAAAGAAGACCCCCCTGATGCAAGGGTCAGTACAGTAACACCTGCAACCATATCTCTCTTCAAAGATATTGGCGCTTGGAAATATGTTGAACAGCATCGACATGCATATTTTGATCAAATGCAGGTTTGGGACTATACTGGCTTAGGATATACGAGATATGATGCTAGAGATGTACATAAAGAATATCTGGGGTGTGTAGTGGAGAATAAAGTGCTGCAGAGTTCTCTTTTGTCATGCATGCAGGATACAGATTTCCAGAAGACTATTTACCCTTCCAGATTATCCTCAATGCCTTTAAATCCAAGCATTCTATCTGTGGGGATGAACAGCACATCATCTGGGTTGAATGAGCGTGGAAACTTAGCAAAACTGGATCTAAGTGATGGCAATAGTTTATATGCAAAGTTGGTGGTGGGAGCTGATGGGTCCAAGTCACGTGTTAGGGAGTTGGCAGGATTTAAGACGACTGGATGGAAGTACTCACAGAATGCAATCATTTGCACAGTAGAGCATCATGCAGAAAATCGATGTGCATGGCAAAGATTTCTACCTACCGGACCAATTGCACTTTTGCCTGTTGGTGATAATTTTAGTAACATTGTTTGGACAATGAACCCGGAAGAAGCAACTGACCGTAAATCAATGGTCGAGGATGATTTTGTGAAAGCTGTAAATTATGCTCTTGATTATGGATTTGGCCCTCATCCTAAGTTGAGCAACTTCGGAAGTGGAGGCATTTTTTCTTGGTTTAAGACAGACACAACTATTTCTGCCAATGAGTGCTTCAAAGTTCCACCGAAAGTGATGAAGTTGGCATCTGAAAGAATGGTGTTTCCCTTGTCTTTGATGCATGCCAATAACTATGTGTCAAAGCATGTGGTTCTAATTGGCGATGCAGCACACACTGTTCACCCTTTGGCTGGGCAAGGAGTTAATCTGGGTTTTGGAGATGCATTTGCTCTTTCAAGAATCATTTCTGAGGGCATTGCAGTGGGAACAGGTATTTCTGAGGTCAATTTGTTGAAGAAATATGAAGCAGAGAGGAGAACGGCTAATGTCACAATGATGGCAATCCTAGATGGTTTCCAAAGGGCTTACTCAGTTGATTTTGGACCTTTAAATGTTCTACGCGCTGCTGCATTCCACGGAGCACAGTACATTCCACCCCTTAAAAGGAGTATAATCTCATATGCATCAGGGGATCAGAGATTGCCAATATTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

510

Amino Acids

55.66

Weight (kDa)

9.0

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 57 - 324 4.8e-07 FAD binding domain
FAD_binding_3 PF01494 377 - 452 1.1e-12 FAD binding domain
SE PF08491 378 - 483 1.5e-06 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 2 cut(s) 352, 415
AccB1I GGYRCC 1 cut(s) 107
AccII CGCG 1 cut(s) 1434
AclWI GGATC 2 cut(s) 684, 1512
AcoI YGGCCR 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 269
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 5 cut(s) 73, 334, 477, 782, 1460
AfiI CCNNNNNNNGG 3 cut(s) 253, 1223, 1476
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 8 cut(s) 49, 150, 364, 392, 649, 1090, 1269, 1327
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AluBI AGCT 7 cut(s) 9, 25, 83, 99, 212, 722, 971
AluI AGCT 7 cut(s) 9, 25, 83, 99, 212, 722, 971
Alw21I GWGCWC 1 cut(s) 1456
AlwI GGATC 2 cut(s) 684, 1512
AoxI GGCC 3 cut(s) 204, 255, 1000
ApeKI GCWGC 7 cut(s) 80, 83, 136, 517, 1199, 1436, 1439
Asp700I GAANNNNTTC 1 cut(s) 1276
AspLEI GCGC 2 cut(s) 377, 1436
AspS9I GGNCC 5 cut(s) 205, 729, 860, 1001, 1415
AsuC2I CCSGG 1 cut(s) 920
AsuHPI GGTGA 2 cut(s) 896, 1205
AvaII GGWCC 3 cut(s) 729, 860, 1415
BaeGI GKGCMC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 315, 569
Bbv12I GWGCWC 1 cut(s) 1456
BbvI GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
BccI CCATC 5 cut(s) 680, 719, 768, 1363, 1376
BceAI ACGGC 1 cut(s) 1368
BciVI GTATCC 1 cut(s) 541
BclI TGATCA 3 cut(s) 12, 124, 415
BcnI CCSGG 1 cut(s) 920
BfaI CTAG 4 cut(s) 209, 225, 468, 1379
BfmI CTRYAG 1 cut(s) 518
BfoI RGCGCY 1 cut(s) 378
BfuAI ACCTGC 2 cut(s) 352, 415
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
BlsI GCNGC 7 cut(s) 82, 85, 138, 519, 1201, 1438, 1441
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 1 cut(s) 920
Bme18I GGWCC 3 cut(s) 729, 860, 1415
BmgT120I GGNCC 5 cut(s) 205, 729, 860, 1001, 1415
BmiI GGNNCC 2 cut(s) 109, 730
BmrFI CCNGG 1 cut(s) 920
BmsI GCATC 9 cut(s) 310, 406, 454, 823, 1127, 1140, 1186, 1243, 1505
BpiI GAAGAC 2 cut(s) 315, 569
Bpu10I CCTNAGC 1 cut(s) 445
BpuMI CCSGG 1 cut(s) 920
Bsa29I ATCGAT 1 cut(s) 829
BsaAI YACGTR 1 cut(s) 740
BsaBI GATNNNNATC 2 cut(s) 270, 1373
BsaJI CCNNGG 2 cut(s) 219, 1447
BsaWI WCCGGW 1 cut(s) 857
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 1223, 1476
Bse1I ACTGG 4 cut(s) 79, 445, 678, 775
Bse3DI GCAATG 3 cut(s) 127, 171, 1287
Bse8I GATNNNNATC 2 cut(s) 270, 1373
BseCI ATCGAT 1 cut(s) 829
BseDI CCNNGG 2 cut(s) 219, 1447
BseGI GGATG 4 cut(s) 630, 779, 961, 1006
BseJI GATNNNNATC 2 cut(s) 270, 1373
BseLI CCNNNNNNNGG 3 cut(s) 253, 1223, 1476
BseMI GCAATG 3 cut(s) 127, 171, 1287
BseMII CTCAG 3 cut(s) 1272, 1302, 1416
BseNI ACTGG 4 cut(s) 79, 445, 678, 775
BseRI GAGGAG 2 cut(s) 201, 1360
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 1 cut(s) 99
Bsh1236I CGCG 1 cut(s) 1434
BshFI GGCC 3 cut(s) 206, 257, 1002
BshNI GGYRCC 1 cut(s) 107
BshVI ATCGAT 1 cut(s) 829
BsiHKAI GWGCWC 1 cut(s) 1456
BsiSI CCGG 2 cut(s) 858, 920
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 3 cut(s) 253, 1223, 1476
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 4 cut(s) 234, 609, 796, 1442
BsnI GGCC 3 cut(s) 206, 257, 1002
Bsp1286I GDGCHC 2 cut(s) 112, 1456
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 5 cut(s) 12, 124, 415, 676, 1504
BspANI GGCC 3 cut(s) 206, 257, 1002
BspCNI CTCAG 3 cut(s) 1273, 1303, 1415
BspDI ATCGAT 1 cut(s) 829
BspFNI CGCG 1 cut(s) 1434
BspLI GGNNCC 2 cut(s) 109, 730
BspMAI CTGCAG 1 cut(s) 522
BspMI ACCTGC 2 cut(s) 352, 415
BspPI GGATC 2 cut(s) 684, 1512
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 3 cut(s) 127, 171, 1287
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 4 cut(s) 79, 445, 678, 775
BssECI CCNNGG 2 cut(s) 219, 1447
BssMI GATC 5 cut(s) 12, 124, 415, 676, 1504
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 6 cut(s) 165, 337, 808, 938, 1210, 1458
Bst6I CTCTTC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 475
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 2 cut(s) 542, 1155
BstDEI CTNAG 7 cut(s) 445, 664, 680, 1011, 1281, 1311, 1402
BstDSI CCRYGG 1 cut(s) 1447
BstF5I GGATG 4 cut(s) 630, 779, 961, 1006
BstFNI CGCG 1 cut(s) 1434
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 2 cut(s) 377, 1436
BstKTI GATC 5 cut(s) 15, 127, 418, 679, 1507
BstMBI GATC 5 cut(s) 12, 124, 415, 676, 1504
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstNSI RCATGY 4 cut(s) 407, 544, 1157, 1181
BstSCI CCNGG 1 cut(s) 918
BstSFI CTRYAG 1 cut(s) 518
BstSLI GKGCMC 1 cut(s) 112
BstUI CGCG 1 cut(s) 1434
BstV1I GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
BstV2I GAAGAC 2 cut(s) 315, 569
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
Bsu15I ATCGAT 1 cut(s) 829
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 3 cut(s) 206, 257, 1002
BsuTUI ATCGAT 1 cut(s) 829
BtgI CCRYGG 1 cut(s) 1447
BtgZI GCGATG 1 cut(s) 1209
BtsCI GGATG 4 cut(s) 630, 779, 961, 1006
BtsI GCAGTG 1 cut(s) 1299
BtsIMutI CAGTG 2 cut(s) 1206, 1299
BveI ACCTGC 2 cut(s) 352, 415
Cac8I GCNNGC 2 cut(s) 542, 1155
CfoI GCGC 2 cut(s) 377, 1436
Cfr13I GGNCC 5 cut(s) 205, 729, 860, 1001, 1415
ClaI ATCGAT 1 cut(s) 829
Csp6I GTAC 5 cut(s) 72, 333, 476, 781, 1459
CspCI CAANNNNNGTGG 4 cut(s) 693, 728, 1161, 1196
CviQI GTAC 5 cut(s) 72, 333, 476, 781, 1459
DdeI CTNAG 7 cut(s) 445, 664, 680, 1011, 1281, 1311, 1402
DpnI GATC 5 cut(s) 14, 126, 417, 678, 1506
DpnII GATC 5 cut(s) 12, 124, 415, 676, 1504
DraI TTTAAA 2 cut(s) 600, 1422
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 219
Eco47I GGWCC 3 cut(s) 729, 860, 1415
Eco57I CTGAAG 1 cut(s) 269
Eco72I CACGTG 1 cut(s) 740
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 6 cut(s) 40, 409, 542, 1155, 1258, 1498
ErhI CCWWGG 1 cut(s) 219
FaqI GGGAC 1 cut(s) 446
FauNDI CATATG 1 cut(s) 1492
FbaI TGATCA 3 cut(s) 12, 124, 415
Fnu4HI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
FokI GGATG 4 cut(s) 637, 786, 968, 993
Fsp4HI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
FspBI CTAG 4 cut(s) 209, 225, 468, 1379
GlaI GCGC 2 cut(s) 376, 1435
GluI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
GsaI CCCAGC 1 cut(s) 1227
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 3 cut(s) 206, 257, 1002
HapII CCGG 2 cut(s) 858, 920
HhaI GCGC 2 cut(s) 377, 1436
Hin6I GCGC 2 cut(s) 375, 1434
HinP1I GCGC 2 cut(s) 375, 1434
HinfI GANTC 2 cut(s) 291, 1272
HpaII CCGG 2 cut(s) 858, 920
HphI GGTGA 2 cut(s) 896, 1205
Hpy166II GTNNAC 1 cut(s) 1213
Hpy188I TCNGA 6 cut(s) 17, 1028, 1123, 1282, 1312, 1509
Hpy188III TCNNGA 5 cut(s) 301, 559, 579, 986, 1269
Hpy8I GTNNAC 1 cut(s) 1213
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 244, 585
HpyCH4III ACNGT 6 cut(s) 165, 337, 808, 938, 1210, 1458
HpyCH4IV ACGT 1 cut(s) 739
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
HpyF3I CTNAG 7 cut(s) 445, 664, 680, 1011, 1281, 1311, 1402
HpySE526I ACGT 1 cut(s) 739
HspAI GCGC 2 cut(s) 375, 1434
Ksp22I TGATCA 3 cut(s) 12, 124, 415
Kzo9I GATC 5 cut(s) 12, 124, 415, 676, 1504
LmnI GCTCC 3 cut(s) 104, 719, 1451
Lsp1109I GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
LweI GCATC 9 cut(s) 310, 406, 454, 823, 1127, 1140, 1186, 1243, 1505
MaeI CTAG 4 cut(s) 209, 225, 468, 1379
MaeII ACGT 1 cut(s) 739
MaeIII GTNAC 4 cut(s) 337, 735, 896, 1360
MalI GATC 5 cut(s) 14, 126, 417, 678, 1506
MboI GATC 5 cut(s) 12, 124, 415, 676, 1504
MboII GAAGA 7 cut(s) 30, 138, 320, 352, 574, 935, 1339
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 2 cut(s) 112, 1456
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 5 cut(s) 864, 889, 976, 1188, 1318
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 9 cut(s) 179, 182, 598, 946, 1014, 1028, 1276, 1306, 1338
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 6 cut(s) 40, 409, 542, 1155, 1258, 1498
MroXI GAANNNNTTC 1 cut(s) 1276
MscI TGGCCA 1 cut(s) 257
MseI TTAA 6 cut(s) 599, 762, 1053, 1236, 1421, 1473
MslI CAYNNNNRTG 3 cut(s) 402, 1292, 1367
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 858, 920
MspR9I CCNGG 1 cut(s) 920
MunI CAATTG 1 cut(s) 864
Mva1269I GAATGC 4 cut(s) 234, 609, 796, 1442
MvnI CGCG 1 cut(s) 1434
MwoI GCNNNNNNNGC 1 cut(s) 198
NciI CCSGG 1 cut(s) 920
NdeI CATATG 1 cut(s) 1492
NdeII GATC 5 cut(s) 12, 124, 415, 676, 1504
NlaIV GGNNCC 2 cut(s) 109, 730
NmuCI GTSAC 2 cut(s) 735, 1360
NsiI ATGCAT 6 cut(s) 40, 409, 542, 1155, 1258, 1498
NspI RCATGY 4 cut(s) 407, 544, 1157, 1181
PaeI GCATGC 2 cut(s) 544, 1157
PaqCI CACCTGC 1 cut(s) 352
PctI GAATGC 4 cut(s) 234, 609, 796, 1442
PdmI GAANNNNTTC 1 cut(s) 1276
PfeI GAWTC 1 cut(s) 1272
PkrI GCNGC 7 cut(s) 82, 85, 138, 519, 1201, 1438, 1441
PleI GAGTC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 1 cut(s) 285
Ppu21I YACGTR 1 cut(s) 740
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 1 cut(s) 1223
PspN4I GGNNCC 2 cut(s) 109, 730
PspPI GGNCC 5 cut(s) 205, 729, 860, 1001, 1415
PstI CTGCAG 1 cut(s) 522
PsuI RGATCY 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 5 cut(s) 73, 334, 477, 782, 1460
RsaNI GTAC 5 cut(s) 72, 333, 476, 781, 1459
RseI CAYNNNNRTG 3 cut(s) 402, 1292, 1367
SaqAI TTAA 6 cut(s) 599, 762, 1053, 1236, 1421, 1473
SatI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
Sau3AI GATC 5 cut(s) 12, 124, 415, 676, 1504
Sau96I GGNCC 5 cut(s) 205, 729, 860, 1001, 1415
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 1 cut(s) 920
SduI GDGCHC 2 cut(s) 112, 1456
SfaNI GCATC 9 cut(s) 310, 406, 454, 823, 1127, 1140, 1186, 1243, 1505
SfcI CTRYAG 1 cut(s) 518
SinI GGWCC 3 cut(s) 729, 860, 1415
SmiMI CAYNNNNRTG 3 cut(s) 402, 1292, 1367
SphI GCATGC 2 cut(s) 544, 1157
Sse9I AATT 5 cut(s) 864, 889, 976, 1188, 1318
SspI AATATT 1 cut(s) 1521
SspMI CTAG 4 cut(s) 209, 225, 468, 1379
StyD4I CCNGG 1 cut(s) 918
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 6 cut(s) 165, 337, 808, 938, 1210, 1458
TaiI ACGT 1 cut(s) 742
TaqI TCGA 4 cut(s) 30, 400, 829, 951
TasI AATT 5 cut(s) 864, 889, 976, 1188, 1318
TatI WGTACW 5 cut(s) 71, 332, 475, 780, 1458
TfiI GAWTC 1 cut(s) 1272
Tru1I TTAA 6 cut(s) 599, 762, 1053, 1236, 1421, 1473
Tru9I TTAA 6 cut(s) 599, 762, 1053, 1236, 1421, 1473
TscAI CASTG 2 cut(s) 1213, 1299
TseFI GTSAC 2 cut(s) 735, 1360
TseI GCWGC 7 cut(s) 80, 83, 136, 517, 1199, 1436, 1439
Tsp45I GTSAC 2 cut(s) 735, 1360
TspDTI ATGAA 5 cut(s) 17, 641, 929, 1124, 1350
TspGWI ACGGA 1 cut(s) 1464
TspRI CASTG 2 cut(s) 1213, 1299
VpaK11BI GGWCC 3 cut(s) 729, 860, 1415
XceI RCATGY 4 cut(s) 407, 544, 1157, 1181
XmnI GAANNNNTTC 1 cut(s) 1276
XspI CTAG 4 cut(s) 209, 225, 468, 1379
ZrmI AGTACT 1 cut(s) 782
Zsp2I ATGCAT 6 cut(s) 40, 409, 542, 1155, 1258, 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.