FvH4_5g03680

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011500
Physical Location & Seq
Forward (+)
2156492 .. 2160967
4476 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03680.t3

Sequence Viewer

Length: 1530 bp
ATGAACAGCTTGATCAGAAGAAAGCTCGTCGAAAATGGATACAGATTGAAAGTTCCACACAAGGGTTTCTGTACTGGTGCAGCTGCTAGAGTTTCCAGCTCCAATGGTGCCCCACCAAGCAATGATCATGTGGGGCAGCAGAAATCTTCAGATAGAGGCGAACCGTATGACGTTGCTATTGTTGGAGGTGGCATGGTTGGGATGGCCTTCGCTTGTTCTTTGGCAAGAATGCCGATGACAAAGCACTTGAAAGTTGCCATCATTGATAGCAATCCTGCATTATCTAGCGGACTCTCAATCAAGAAAGAAGACCCCCCTGATGCAAGGGTCAGTACAGTAACACCTGCAACTATATCTCTCTTCAAAGATATTGGCGCTTGGAAATATGTTGAACAGCATCGACATGCATATTTTGATCAAATGCAGGTTTGGGACTATACTGGCTTAGGATATACGAGATACGATGCAAGAGATGTACATAAAGAATATCTGGGGTGTGTAGTGGAGAATAAAGTGCTGCAGAGTTCTCTTTTGTCATGCATGCAGGATACAGATTTCCAGAAGACTATTTACCCTTCCATATTGTCCTCAATGGCTTTAAATCCAAGCATTCTATCTATGGGGGTGAAAAGCACATCATCTGGGTTGAATGAGCGAGGAAATTTAGCAAAACTGGATCTAAGTGATGGCAATAGTCTATATGCAAAGTTGGTGGTTGGAGCTGATGGGTCCAAGTCACGTGTTAGGGAGTTAGCAGGATTCAAAACGACTGGTTGGAAATACTCACAGAATGCAATCATTTGCACTGTAGAGCATACTGCAGAAAATCGAAGTGCATGGCAAAGATTCCTACCTACCGGACCAATTGCACTTTTGCCCATTGGTGATAATTTCAGTAACATTGTTTGGACTATGAACCCACAAGAAGCAACTGACCGTAAATTAATGGTCGAGGATGATTTTGTGAAAAACGTAAATTATGCTCTTGATTATGGATTTGGCCCTCATCCTAAGTCGAGCAACTTTGGAAGTGGAGGAATATTTTCTTGGTTTAAGACAGACACAACTATTTCTGCTAATGAGTGCTTCAAAGTTCCACTGAAAGTGGTGAAGTTGGCATCTGAAAGAATGGTGTTTCCCTTGTCTTTGATGCATGCCAATAACTATGCGTCAAAGCATGTGGTTCTAATTGGCGATGCAGCACACACTGTTCACCCTTTGGCTGGGCAAGGAGTTAATCTGGGTTTTGGTGATGCATTTGCTCTTTCAAGAATAATTTCTGAGGGCATTGCAGTGGGAACAGATATTGCTGAGGTCAATTTGTTAAAGAGATATGAAGCAGAGAGGAAAACGGCTAATGTTACAATGATGGCAATCCTAGATGGTTTCCAAAGGGCTTACTCAGTTGATTTTGGACCTTTAAATGTTCTACGTGCTGCTGCATTCCACGGAGCACAGTACATTCCACCTCTTAAAAGGAGTATAATCTCATATGCATCAGGGGACCAGAGATTGCCAATATTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

510

Amino Acids

55.59

Weight (kDa)

8.97

Isoelectric Point (pI)

29.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 196 - 272 4.6e-13 FAD binding domain
SE PF08491 198 - 303 3.2e-08 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 352
Acc36I ACCTGC 2 cut(s) 352, 415
AccB1I GGYRCC 1 cut(s) 107
AciI CCGC 1 cut(s) 288
AclWI GGATC 1 cut(s) 684
AcsI RAATTY 1 cut(s) 661
AcuI CTGAAG 1 cut(s) 132
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 4 cut(s) 73, 334, 477, 1460
AfiI CCNNNNNNNGG 2 cut(s) 62, 1223
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 8 cut(s) 49, 250, 364, 392, 649, 763, 1090, 1269
AjuI GAANNNNNNNTTGG 2 cut(s) 1030, 1062
AluBI AGCT 5 cut(s) 9, 25, 83, 99, 722
AluI AGCT 5 cut(s) 9, 25, 83, 99, 722
Alw21I GWGCWC 1 cut(s) 1456
AlwI GGATC 1 cut(s) 684
AoxI GGCC 2 cut(s) 204, 1000
ApeKI GCWGC 7 cut(s) 80, 83, 136, 517, 1199, 1436, 1439
ApoI RAATTY 1 cut(s) 661
AseI ATTAAT 1 cut(s) 944
Asp700I GAANNNNTTC 2 cut(s) 1042, 1276
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 5 cut(s) 729, 860, 1001, 1415, 1504
AsuHPI GGTGA 5 cut(s) 637, 896, 1120, 1205, 1262
AvaII GGWCC 4 cut(s) 729, 860, 1415, 1504
BaeGI GKGCMC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 107
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 315, 569
Bbv12I GWGCWC 1 cut(s) 1456
BbvCI CCTCAGC 1 cut(s) 1311
BbvI GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
BccI CCATC 6 cut(s) 196, 266, 680, 719, 1363, 1376
BceAI ACGGC 1 cut(s) 1368
BciVI GTATCC 2 cut(s) 32, 541
BclI TGATCA 3 cut(s) 12, 124, 415
BfaI CTAG 3 cut(s) 87, 285, 1379
BfmI CTRYAG 3 cut(s) 518, 807, 819
BfoI RGCGCY 1 cut(s) 378
BfuAI ACCTGC 2 cut(s) 352, 415
BfuI GTATCC 2 cut(s) 32, 541
BisI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
BlsI GCNGC 7 cut(s) 82, 85, 138, 519, 1201, 1438, 1441
Bme18I GGWCC 4 cut(s) 729, 860, 1415, 1504
BmgT120I GGNCC 5 cut(s) 729, 860, 1001, 1415, 1504
BmiI GGNNCC 3 cut(s) 109, 730, 1505
BmsI GCATC 8 cut(s) 310, 406, 454, 1127, 1140, 1186, 1243, 1505
BpiI GAAGAC 2 cut(s) 315, 569
Bpu10I CCTNAGC 2 cut(s) 445, 1311
BsaAI YACGTR 2 cut(s) 740, 1433
BsaBI GATNNNNATC 2 cut(s) 270, 1373
BsaJI CCNNGG 1 cut(s) 1447
BsaWI WCCGGW 1 cut(s) 857
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 1223
Bse1I ACTGG 4 cut(s) 79, 445, 678, 775
Bse3DI GCAATG 2 cut(s) 127, 1287
Bse8I GATNNNNATC 2 cut(s) 270, 1373
BseDI CCNNGG 1 cut(s) 1447
BseGI GGATG 3 cut(s) 207, 961, 1006
BseJI GATNNNNATC 2 cut(s) 270, 1373
BseLI CCNNNNNNNGG 2 cut(s) 62, 1223
BseMI GCAATG 2 cut(s) 127, 1287
BseMII CTCAG 3 cut(s) 1272, 1302, 1416
BseNI ACTGG 4 cut(s) 79, 445, 678, 775
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 1 cut(s) 99
BshFI GGCC 2 cut(s) 206, 1002
BshNI GGYRCC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1456
BsiSI CCGG 1 cut(s) 858
BslFI GGGAC 2 cut(s) 446, 1517
BslI CCNNNNNNNGG 2 cut(s) 62, 1223
BsmFI GGGAC 2 cut(s) 446, 1517
BsmI GAATGC 4 cut(s) 234, 609, 796, 1442
BsnI GGCC 2 cut(s) 206, 1002
Bsp1286I GDGCHC 2 cut(s) 112, 1456
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 4 cut(s) 12, 124, 415, 676
BspACI CCGC 1 cut(s) 288
BspANI GGCC 2 cut(s) 206, 1002
BspCNI CTCAG 3 cut(s) 1273, 1303, 1415
BspLI GGNNCC 3 cut(s) 109, 730, 1505
BspMAI CTGCAG 2 cut(s) 522, 823
BspMI ACCTGC 2 cut(s) 352, 415
BspPI GGATC 1 cut(s) 684
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 2 cut(s) 127, 1287
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 4 cut(s) 79, 445, 678, 775
BssECI CCNNGG 1 cut(s) 1447
BssMI GATC 4 cut(s) 12, 124, 415, 676
Bst4CI ACNGT 6 cut(s) 165, 337, 808, 938, 1210, 1458
Bst6I CTCTTC 1 cut(s) 365
BstAUI TGTACA 1 cut(s) 475
BstBAI YACGTR 2 cut(s) 740, 1433
BstC8I GCNNGC 2 cut(s) 542, 1155
BstDEI CTNAG 6 cut(s) 445, 680, 1011, 1281, 1311, 1402
BstDSI CCRYGG 1 cut(s) 1447
BstF5I GGATG 3 cut(s) 207, 961, 1006
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 4 cut(s) 15, 127, 418, 679
BstMBI GATC 4 cut(s) 12, 124, 415, 676
BstNSI RCATGY 4 cut(s) 407, 544, 1157, 1181
BstSFI CTRYAG 3 cut(s) 518, 807, 819
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
BstV2I GAAGAC 2 cut(s) 315, 569
BstX2I RGATCY 1 cut(s) 676
BstYI RGATCY 1 cut(s) 676
BsuI GTATCC 2 cut(s) 32, 541
BsuRI GGCC 2 cut(s) 206, 1002
BtgI CCRYGG 1 cut(s) 1447
BtgZI GCGATG 1 cut(s) 1209
BtsCI GGATG 3 cut(s) 207, 961, 1006
BtsI GCAGTG 1 cut(s) 1299
BtsIMutI CAGTG 4 cut(s) 804, 1097, 1206, 1299
BveI ACCTGC 2 cut(s) 352, 415
Cac8I GCNNGC 2 cut(s) 542, 1155
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 5 cut(s) 729, 860, 1001, 1415, 1504
CseI GACGC 1 cut(s) 1158
Csp6I GTAC 4 cut(s) 72, 333, 476, 1459
CspCI CAANNNNNGTGG 4 cut(s) 693, 728, 1161, 1196
CviAII CATG 8 cut(s) 128, 193, 404, 537, 541, 837, 1154, 1178
CviQI GTAC 4 cut(s) 72, 333, 476, 1459
DdeI CTNAG 6 cut(s) 445, 680, 1011, 1281, 1311, 1402
DpnI GATC 4 cut(s) 14, 126, 417, 678
DpnII GATC 4 cut(s) 12, 124, 415, 676
DraI TTTAAA 2 cut(s) 600, 1422
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
Eco47I GGWCC 4 cut(s) 729, 860, 1415, 1504
Eco57I CTGAAG 1 cut(s) 132
Eco72I CACGTG 1 cut(s) 740
EcoT22I ATGCAT 5 cut(s) 409, 542, 1155, 1258, 1498
FaeI CATG 8 cut(s) 131, 196, 407, 540, 544, 840, 1157, 1181
FaqI GGGAC 2 cut(s) 446, 1517
FatI CATG 8 cut(s) 127, 192, 403, 536, 540, 836, 1153, 1177
FauNDI CATATG 1 cut(s) 1492
FbaI TGATCA 3 cut(s) 12, 124, 415
Fnu4HI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
FokI GGATG 3 cut(s) 214, 968, 993
Fsp4HI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
FspBI CTAG 3 cut(s) 87, 285, 1379
GlaI GCGC 1 cut(s) 376
GluI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
GsaI CCCAGC 1 cut(s) 1227
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 2 cut(s) 206, 1002
HapII CCGG 1 cut(s) 858
HgaI GACGC 1 cut(s) 1158
HhaI GCGC 1 cut(s) 377
Hin1II CATG 8 cut(s) 131, 196, 407, 540, 544, 840, 1157, 1181
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HinfI GANTC 3 cut(s) 291, 759, 846
HpaII CCGG 1 cut(s) 858
HphI GGTGA 5 cut(s) 637, 896, 1120, 1205, 1262
Hpy166II GTNNAC 1 cut(s) 1213
Hpy188I TCNGA 4 cut(s) 17, 151, 1123, 1282
Hpy188III TCNNGA 5 cut(s) 301, 559, 986, 1269, 1527
Hpy8I GTNNAC 1 cut(s) 1213
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 217, 585
HpyCH4III ACNGT 6 cut(s) 165, 337, 808, 938, 1210, 1458
HpyCH4IV ACGT 4 cut(s) 171, 739, 972, 1432
HpyF3I CTNAG 6 cut(s) 445, 680, 1011, 1281, 1311, 1402
HpySE526I ACGT 4 cut(s) 171, 739, 972, 1432
Hsp92II CATG 8 cut(s) 131, 196, 407, 540, 544, 840, 1157, 1181
HspAI GCGC 1 cut(s) 375
Ksp22I TGATCA 3 cut(s) 12, 124, 415
Kzo9I GATC 4 cut(s) 12, 124, 415, 676
LmnI GCTCC 3 cut(s) 104, 719, 1451
Lsp1109I GCAGC 7 cut(s) 70, 92, 148, 504, 1211, 1423, 1426
LweI GCATC 8 cut(s) 310, 406, 454, 1127, 1140, 1186, 1243, 1505
MaeI CTAG 3 cut(s) 87, 285, 1379
MaeII ACGT 4 cut(s) 171, 739, 972, 1432
MaeIII GTNAC 4 cut(s) 337, 735, 896, 1360
MalI GATC 4 cut(s) 14, 126, 417, 678
MboI GATC 4 cut(s) 12, 124, 415, 676
MboII GAAGA 5 cut(s) 30, 138, 320, 352, 574
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 1 cut(s) 676
MhlI GDGCHC 2 cut(s) 112, 1456
MluCI AATT 8 cut(s) 661, 864, 889, 941, 976, 1188, 1275, 1318
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 3 cut(s) 163, 697, 755
Mph1103I ATGCAT 5 cut(s) 409, 542, 1155, 1258, 1498
MroXI GAANNNNTTC 2 cut(s) 1042, 1276
MseI TTAA 7 cut(s) 599, 944, 1053, 1236, 1325, 1421, 1473
MslI CAYNNNNRTG 2 cut(s) 402, 1292
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 858
MunI CAATTG 1 cut(s) 864
Mva1269I GAATGC 4 cut(s) 234, 609, 796, 1442
NdeI CATATG 1 cut(s) 1492
NdeII GATC 4 cut(s) 12, 124, 415, 676
NlaIII CATG 8 cut(s) 131, 196, 407, 540, 544, 840, 1157, 1181
NlaIV GGNNCC 3 cut(s) 109, 730, 1505
NmuCI GTSAC 1 cut(s) 735
NsiI ATGCAT 5 cut(s) 409, 542, 1155, 1258, 1498
NspI RCATGY 4 cut(s) 407, 544, 1157, 1181
PaeI GCATGC 2 cut(s) 544, 1157
PaqCI CACCTGC 1 cut(s) 352
PctI GAATGC 4 cut(s) 234, 609, 796, 1442
PdmI GAANNNNTTC 2 cut(s) 1042, 1276
PfeI GAWTC 2 cut(s) 759, 846
PkrI GCNGC 7 cut(s) 82, 85, 138, 519, 1201, 1438, 1441
PleI GAGTC 1 cut(s) 285
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 1 cut(s) 285
Ppu21I YACGTR 2 cut(s) 740, 1433
PshBI ATTAAT 1 cut(s) 944
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 1 cut(s) 1223
PspN4I GGNNCC 3 cut(s) 109, 730, 1505
PspPI GGNCC 5 cut(s) 729, 860, 1001, 1415, 1504
PstI CTGCAG 2 cut(s) 522, 823
PsuI RGATCY 1 cut(s) 676
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 4 cut(s) 73, 334, 477, 1460
RsaNI GTAC 4 cut(s) 72, 333, 476, 1459
RseI CAYNNNNRTG 2 cut(s) 402, 1292
SaqAI TTAA 7 cut(s) 599, 944, 1053, 1236, 1325, 1421, 1473
SatI GCNGC 7 cut(s) 81, 84, 137, 518, 1200, 1437, 1440
Sau3AI GATC 4 cut(s) 12, 124, 415, 676
Sau96I GGNCC 5 cut(s) 729, 860, 1001, 1415, 1504
SchI GAGTC 1 cut(s) 285
SduI GDGCHC 2 cut(s) 112, 1456
SfaNI GCATC 8 cut(s) 310, 406, 454, 1127, 1140, 1186, 1243, 1505
SfcI CTRYAG 3 cut(s) 518, 807, 819
SinI GGWCC 4 cut(s) 729, 860, 1415, 1504
SmiMI CAYNNNNRTG 2 cut(s) 402, 1292
SphI GCATGC 2 cut(s) 544, 1157
Sse9I AATT 8 cut(s) 661, 864, 889, 941, 976, 1188, 1275, 1318
SsiI CCGC 1 cut(s) 288
SspI AATATT 2 cut(s) 1041, 1521
SspMI CTAG 3 cut(s) 87, 285, 1379
TaaI ACNGT 6 cut(s) 165, 337, 808, 938, 1210, 1458
TaiI ACGT 4 cut(s) 174, 742, 975, 1435
TaqI TCGA 5 cut(s) 30, 400, 829, 951, 1016
TasI AATT 8 cut(s) 661, 864, 889, 941, 976, 1188, 1275, 1318
TatI WGTACW 4 cut(s) 71, 332, 475, 1458
TfiI GAWTC 2 cut(s) 759, 846
Tru1I TTAA 7 cut(s) 599, 944, 1053, 1236, 1325, 1421, 1473
Tru9I TTAA 7 cut(s) 599, 944, 1053, 1236, 1325, 1421, 1473
TscAI CASTG 4 cut(s) 811, 1104, 1213, 1299
TseFI GTSAC 1 cut(s) 735
TseI GCWGC 7 cut(s) 80, 83, 136, 517, 1199, 1436, 1439
Tsp45I GTSAC 1 cut(s) 735
TspDTI ATGAA 3 cut(s) 17, 929, 1350
TspGWI ACGGA 1 cut(s) 1464
TspRI CASTG 4 cut(s) 811, 1104, 1213, 1299
VpaK11BI GGWCC 4 cut(s) 729, 860, 1415, 1504
VspI ATTAAT 1 cut(s) 944
XapI RAATTY 1 cut(s) 661
XceI RCATGY 4 cut(s) 407, 544, 1157, 1181
XmnI GAANNNNTTC 2 cut(s) 1042, 1276
XspI CTAG 3 cut(s) 87, 285, 1379
Zsp2I ATGCAT 5 cut(s) 409, 542, 1155, 1258, 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.