MD14G1116500.v1.1

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011500
Physical Location & Seq
Forward (+)
18917208 .. 18922055
4848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1116500.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGGTGCCCAACCCAGCAATGATCATGTGGAGAGGCCAAATTTTTCGAATAAATGTGATCCCTACGACATTGCTATTATTGGAGGAGGGATGGTTGGCATGGCCCTTGCTTGTTCCTTGGGTAACAAAAATGCCATTGACAAAGCACTTGAAAGTTGCCATCATTGATAGCAATCCTGCAGTATCAAATGGACTTAGCATCAAGAAAGAAGACCCCCCTGATCCAAGGGCCAGTGCAGTAGCACCTGCAACCATATCTCTCTTCCAAGATATTGGTGCTTGGAAATATGTGGAACAGAACAGGCATGCGTATTTTGATCAAATGCAGGTTTGGGACTATACTGGCTTAGGTTATACAAGATACAATGCAAGAGATGTACATAAAGATTCTCTAGGGTGTGTAGTGGAGAATAAAGTACTGCACAGTTCCCTATTGTCATGTATGCAGAATACAGATTTCCAGAAGACTGTCTACCCTTCGAGATTGTCTACAATGACTTTACAGCCAAGAAACTTATCCATGGGGATGGAAAGCACATCATCTGGATTGAATGAGCAGGGGAACTTAGCGAAGCTTGATCTAATTGACGGCAACAGTCTGTATGCAAAGTTGGTGGTGGGAGCTGATGGGTCCAAGTCACGTGTTAGAGAGTTGGCAGGATTCAAGACAACCGGATGGAATTACTCGCAGAACGCAATCATTTGTACTGTTGAACATAGTGTAGAAAACCGATTTGCATGGCAACGCTTTCTACCTGCTGGGCCAATTGCACTTTTGCCAATAGGTGATAATTTCAGTAACATTGTTTGGACCATGAACCCGAACGAAGCAACTGACCGCAAGTTAAAGGCTGAGGATGAATTTTTGAAAGATGTCAATTATGCTCTGGATTATGGATTTGGCCCTCATCCTAAGTCAAGCACCTCGGGAGGTGGAAGCATATTTTCTTGGTTTAAAACAGACACGAATTTATCAGCCAATGACTGCTTCAAAGTTCCGCCAAAAGTGTTGAAGTTGGCATCTGAGAGAACGGTGTTTCCTTTGTCTTTGATGCATGCCAACGACTACGTGTCAAAGCATGTGGTTCTAATTGGCGATGCAGCACATACCGTTCACCCTTTGGCGGGCAAGGGGTTAATTTGGGGTTTGGAGACGCATTTGCTCTATCAAGAATCATTTGTATTGTTGTTGAAGAAATATGAAGCAGAGAGGAAATCGGCAAATGTCACAATGATGGCAATCCTGGACGGTTTCCAGAAGGCTTACTCTGTTGATTTCGGACCTTTAAATGTACTGCGGCCTGCAGCATTCAACGGAGCGCAGTACATTCCACCCCTTAAGAGGAGTATCATTTCATATGCCCTCAGGGGATCAAAGATTGCCAATTTTTTCTTGAGACGGACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

469

Amino Acids

52.22

Weight (kDa)

9.08

Isoelectric Point (pI)

27.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 71 - 291 5.4e-06 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
Acc36I ACCTGC 3 cut(s) 253, 316, 763
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 471, 488
AciI CCGC 4 cut(s) 838, 998, 1124, 1297
AclWI GGATC 3 cut(s) 52, 215, 1378
AcsI RAATTY 3 cut(s) 39, 860, 967
AcvI CACGTG 1 cut(s) 641
AfaI GTAC 5 cut(s) 378, 417, 706, 1293, 1325
AfiI CCNNNNNNNGG 3 cut(s) 1123, 1124, 1341
AflII CTTAAG 1 cut(s) 1337
AflIII ACRYGT 2 cut(s) 640, 1068
AgsI TTSAA 9 cut(s) 151, 550, 664, 713, 868, 991, 1012, 1192, 1312
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 1 cut(s) 1242
AjuI GAANNNNNNNTTGG 2 cut(s) 971, 1003
AloI GAACNNNNNNTCC 2 cut(s) 1021, 1053
AluBI AGCT 2 cut(s) 574, 623
AluI AGCT 2 cut(s) 574, 623
Alw26I GTCTC 2 cut(s) 1145, 1390
AlwI GGATC 3 cut(s) 52, 215, 1378
Ama87I CYCGRG 1 cut(s) 925
AoxI GGCC 6 cut(s) 34, 101, 228, 761, 901, 1298
ApeKI GCWGC 2 cut(s) 1100, 1304
ApoI RAATTY 3 cut(s) 39, 860, 967
AspLEI GCGC 1 cut(s) 1321
AspS9I GGNCC 7 cut(s) 102, 228, 630, 761, 810, 902, 1280
AsuHPI GGTGA 2 cut(s) 797, 1106
AsuII TTCGAA 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 925
AvaII GGWCC 3 cut(s) 630, 810, 1280
AxyI CCTNAGG 1 cut(s) 1364
BaeGI GKGCMC 1 cut(s) 9
BanI GGYRCC 1 cut(s) 4
BarI GAAGNNNNNNTAC 2 cut(s) 455, 487
BbrPI CACGTG 1 cut(s) 641
BbsI GAAGAC 2 cut(s) 216, 470
BbvCI CCTCAGC 1 cut(s) 852
BbvI GCAGC 2 cut(s) 1112, 1316
BccI CCATC 6 cut(s) 84, 167, 520, 620, 669, 1228
BceAI ACGGC 1 cut(s) 604
BciT130I CCWGG 1 cut(s) 1244
BclI TGATCA 2 cut(s) 21, 316
BcoDI GTCTC 2 cut(s) 1145, 1390
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 2 cut(s) 177, 1302
BfrI CTTAAG 1 cut(s) 1337
BfuAI ACCTGC 3 cut(s) 253, 316, 763
BisI GCNGC 3 cut(s) 1101, 1298, 1305
BlsI GCNGC 3 cut(s) 1102, 1299, 1306
BmcAI AGTACT 1 cut(s) 417
Bme1390I CCNGG 1 cut(s) 1244
Bme18I GGWCC 3 cut(s) 630, 810, 1280
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmeT110I CYCGRG 1 cut(s) 925
BmgT120I GGNCC 7 cut(s) 102, 228, 630, 761, 810, 902, 1280
BmiI GGNNCC 2 cut(s) 6, 631
BmrFI CCNGG 1 cut(s) 1244
BmsI GCATC 4 cut(s) 207, 1028, 1041, 1087
BpiI GAAGAC 2 cut(s) 216, 470
Bpu10I CCTNAGC 2 cut(s) 346, 852
Bpu14I TTCGAA 1 cut(s) 46
BsaAI YACGTR 2 cut(s) 641, 1069
BsaBI GATNNNNATC 2 cut(s) 171, 1238
BsaJI CCNNGG 4 cut(s) 116, 224, 519, 924
BsaWI WCCGGW 1 cut(s) 671
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 3 cut(s) 1123, 1124, 1341
Bse1I ACTGG 2 cut(s) 231, 346
Bse21I CCTNAGG 1 cut(s) 1364
Bse3DI GCAATG 2 cut(s) 24, 68
Bse8I GATNNNNATC 2 cut(s) 171, 1238
BseBI CCWGG 1 cut(s) 1244
BseDI CCNNGG 4 cut(s) 116, 224, 519, 924
BseGI GGATG 5 cut(s) 95, 531, 680, 862, 907
BseJI GATNNNNATC 2 cut(s) 171, 1238
BseLI CCNNNNNNNGG 3 cut(s) 1123, 1124, 1341
BseMI GCAATG 2 cut(s) 24, 68
BseMII CTCAG 3 cut(s) 843, 1014, 1378
BseNI ACTGG 2 cut(s) 231, 346
BseRI GAGGAG 2 cut(s) 98, 1357
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 2 cut(s) 1112, 1316
BseYI CCCAGC 2 cut(s) 13, 758
BsgI GTGCAG 2 cut(s) 255, 404
BshFI GGCC 6 cut(s) 36, 103, 230, 763, 903, 1300
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 925
BsiSI CCGG 1 cut(s) 672
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 3 cut(s) 1123, 1124, 1341
BsmAI GTCTC 2 cut(s) 1145, 1390
BsmBI CGTCTC 2 cut(s) 1145, 1390
BsmFI GGGAC 1 cut(s) 347
BsmI GAATGC 1 cut(s) 1307
BsnI GGCC 6 cut(s) 36, 103, 230, 763, 903, 1300
BsoBI CYCGRG 1 cut(s) 925
Bsp119I TTCGAA 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 376
Bsp143I GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 4 cut(s) 838, 998, 1124, 1297
BspANI GGCC 6 cut(s) 36, 103, 230, 763, 903, 1300
BspCNI CTCAG 3 cut(s) 844, 1015, 1377
BspLI GGNNCC 2 cut(s) 6, 631
BspMAI CTGCAG 2 cut(s) 181, 1306
BspMI ACCTGC 3 cut(s) 253, 316, 763
BspPI GGATC 3 cut(s) 52, 215, 1378
BspT104I TTCGAA 1 cut(s) 46
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 1337
BsrDI GCAATG 2 cut(s) 24, 68
BsrGI TGTACA 1 cut(s) 376
BsrI ACTGG 2 cut(s) 231, 346
BssECI CCNNGG 4 cut(s) 116, 224, 519, 924
BssMI GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
BssT1I CCWWGG 3 cut(s) 116, 224, 519
Bst2UI CCWGG 1 cut(s) 1244
Bst4CI ACNGT 7 cut(s) 425, 469, 596, 709, 1033, 1111, 1250
Bst6I CTCTTC 1 cut(s) 266
BstAFI CTTAAG 1 cut(s) 1337
BstAUI TGTACA 1 cut(s) 376
BstBAI YACGTR 2 cut(s) 641, 1069
BstBI TTCGAA 1 cut(s) 46
BstC8I GCNNGC 4 cut(s) 306, 1056, 1126, 1302
BstDEI CTNAG 7 cut(s) 194, 346, 565, 852, 912, 1023, 1364
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 5 cut(s) 95, 531, 680, 862, 907
BstHHI GCGC 1 cut(s) 1321
BstKTI GATC 6 cut(s) 24, 60, 223, 319, 580, 1373
BstMAI GTCTC 2 cut(s) 1145, 1390
BstMBI GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
BstNI CCWGG 1 cut(s) 1244
BstNSI RCATGY 3 cut(s) 308, 1058, 1082
BstSCI CCNGG 1 cut(s) 1242
BstSFI CTRYAG 2 cut(s) 177, 1302
BstSLI GKGCMC 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 1112, 1316
BstV2I GAAGAC 2 cut(s) 216, 470
BstXI CCANNNNNNTGG 2 cut(s) 272, 526
Bsu36I CCTNAGG 1 cut(s) 1364
BsuRI GGCC 6 cut(s) 36, 103, 230, 763, 903, 1300
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 1 cut(s) 1110
BtsCI GGATG 5 cut(s) 95, 531, 680, 862, 907
BtsIMutI CAGTG 1 cut(s) 238
BveI ACCTGC 3 cut(s) 253, 316, 763
Cac8I GCNNGC 4 cut(s) 306, 1056, 1126, 1302
CfoI GCGC 1 cut(s) 1321
Cfr13I GGNCC 7 cut(s) 102, 228, 630, 761, 810, 902, 1280
CseI GACGC 1 cut(s) 1162
Csp6I GTAC 5 cut(s) 377, 416, 705, 1292, 1324
CspCI CAANNNNNGTGG 4 cut(s) 594, 629, 1062, 1097
CviAII CATG 9 cut(s) 25, 99, 305, 438, 520, 738, 814, 1055, 1079
CviQI GTAC 5 cut(s) 377, 416, 705, 1292, 1324
DdeI CTNAG 7 cut(s) 194, 346, 565, 852, 912, 1023, 1364
DpnI GATC 6 cut(s) 23, 59, 222, 318, 579, 1372
DpnII GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
DraI TTTAAA 2 cut(s) 955, 1287
DriI GACNNNNNGTC 1 cut(s) 1069
Eam1104I CTCTTC 1 cut(s) 266
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 987
Eco130I CCWWGG 3 cut(s) 116, 224, 519
Eco47I GGWCC 3 cut(s) 630, 810, 1280
Eco72I CACGTG 1 cut(s) 641
Eco81I CCTNAGG 1 cut(s) 1364
Eco88I CYCGRG 1 cut(s) 925
EcoRII CCWGG 1 cut(s) 1242
EcoT14I CCWWGG 3 cut(s) 116, 224, 519
EcoT22I ATGCAT 1 cut(s) 1056
ErhI CCWWGG 3 cut(s) 116, 224, 519
Esp3I CGTCTC 2 cut(s) 1145, 1390
FaeI CATG 9 cut(s) 28, 102, 308, 441, 523, 741, 817, 1058, 1082
FaqI GGGAC 1 cut(s) 347
FatI CATG 9 cut(s) 24, 98, 304, 437, 519, 737, 813, 1054, 1078
FauI CCCGC 1 cut(s) 1117
FauNDI CATATG 1 cut(s) 1357
FbaI TGATCA 2 cut(s) 21, 316
FblI GTMKAC 2 cut(s) 471, 488
Fnu4HI GCNGC 3 cut(s) 1101, 1298, 1305
FokI GGATG 5 cut(s) 102, 538, 687, 869, 894
Fsp4HI GCNGC 3 cut(s) 1101, 1298, 1305
FspBI CTAG 1 cut(s) 392
GlaI GCGC 1 cut(s) 1320
GluI GCNGC 3 cut(s) 1101, 1298, 1305
GsaI CCCAGC 2 cut(s) 17, 762
HaeIII GGCC 6 cut(s) 36, 103, 230, 763, 903, 1300
HapII CCGG 1 cut(s) 672
HgaI GACGC 1 cut(s) 1162
HhaI GCGC 1 cut(s) 1321
Hin1II CATG 9 cut(s) 28, 102, 308, 441, 523, 741, 817, 1058, 1082
Hin6I GCGC 1 cut(s) 1319
HinP1I GCGC 1 cut(s) 1319
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 3 cut(s) 386, 660, 1172
HpaII CCGG 1 cut(s) 672
HphI GGTGA 2 cut(s) 797, 1106
Hpy166II GTNNAC 3 cut(s) 472, 489, 1114
Hpy188I TCNGA 2 cut(s) 1024, 1280
Hpy8I GTNNAC 3 cut(s) 472, 489, 1114
HpyAV CCTTC 2 cut(s) 486, 1252
HpyCH4III ACNGT 7 cut(s) 425, 469, 596, 709, 1033, 1111, 1250
HpyCH4IV ACGT 3 cut(s) 640, 1068, 1403
HpyF3I CTNAG 7 cut(s) 194, 346, 565, 852, 912, 1023, 1364
HpySE526I ACGT 3 cut(s) 640, 1068, 1403
Hsp92II CATG 9 cut(s) 28, 102, 308, 441, 523, 741, 817, 1058, 1082
HspAI GCGC 1 cut(s) 1319
Ksp22I TGATCA 2 cut(s) 21, 316
Kzo9I GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
LmnI GCTCC 2 cut(s) 620, 1316
Lsp1109I GCAGC 2 cut(s) 1112, 1316
LweI GCATC 4 cut(s) 207, 1028, 1041, 1087
MaeI CTAG 1 cut(s) 392
MaeII ACGT 3 cut(s) 640, 1068, 1403
MaeIII GTNAC 4 cut(s) 121, 636, 797, 1225
MalI GATC 6 cut(s) 23, 59, 222, 318, 579, 1372
MboI GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
MboII GAAGA 4 cut(s) 221, 253, 475, 1204
MfeI CAATTG 1 cut(s) 765
MhlI GDGCHC 1 cut(s) 9
Mph1103I ATGCAT 1 cut(s) 1056
MseI TTAA 5 cut(s) 845, 954, 1136, 1286, 1338
MslI CAYNNNNRTG 2 cut(s) 524, 1232
MspCI CTTAAG 1 cut(s) 1337
MspI CCGG 1 cut(s) 672
MspR9I CCNGG 1 cut(s) 1244
MunI CAATTG 1 cut(s) 765
Mva1269I GAATGC 1 cut(s) 1307
MvaI CCWGG 1 cut(s) 1244
NcoI CCATGG 1 cut(s) 519
NdeI CATATG 1 cut(s) 1357
NdeII GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
NlaIII CATG 9 cut(s) 28, 102, 308, 441, 523, 741, 817, 1058, 1082
NlaIV GGNNCC 2 cut(s) 6, 631
NmuCI GTSAC 2 cut(s) 636, 1225
NsiI ATGCAT 1 cut(s) 1056
NspI RCATGY 3 cut(s) 308, 1058, 1082
NspV TTCGAA 1 cut(s) 46
PaeI GCATGC 2 cut(s) 308, 1058
PaqCI CACCTGC 1 cut(s) 253
PctI GAATGC 1 cut(s) 1307
PfeI GAWTC 3 cut(s) 386, 660, 1172
PfoI TCCNGGA 1 cut(s) 1242
PkrI GCNGC 3 cut(s) 1102, 1299, 1306
PmaCI CACGTG 1 cut(s) 641
PmlI CACGTG 1 cut(s) 641
Ppu21I YACGTR 2 cut(s) 641, 1069
Psp6I CCWGG 1 cut(s) 1242
PspCI CACGTG 1 cut(s) 641
PspFI CCCAGC 2 cut(s) 13, 758
PspGI CCWGG 1 cut(s) 1242
PspN4I GGNNCC 2 cut(s) 6, 631
PspPI GGNCC 7 cut(s) 102, 228, 630, 761, 810, 902, 1280
PstI CTGCAG 2 cut(s) 181, 1306
RsaI GTAC 5 cut(s) 378, 417, 706, 1293, 1325
RsaNI GTAC 5 cut(s) 377, 416, 705, 1292, 1324
RseI CAYNNNNRTG 2 cut(s) 524, 1232
SaqAI TTAA 5 cut(s) 845, 954, 1136, 1286, 1338
SatI GCNGC 3 cut(s) 1101, 1298, 1305
Sau3AI GATC 6 cut(s) 21, 57, 220, 316, 577, 1370
Sau96I GGNCC 7 cut(s) 102, 228, 630, 761, 810, 902, 1280
ScaI AGTACT 1 cut(s) 417
ScrFI CCNGG 1 cut(s) 1244
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 4 cut(s) 207, 1028, 1041, 1087
SfcI CTRYAG 2 cut(s) 177, 1302
SfuI TTCGAA 1 cut(s) 46
SinI GGWCC 3 cut(s) 630, 810, 1280
SmiMI CAYNNNNRTG 2 cut(s) 524, 1232
SmlI CTYRAG 2 cut(s) 1337, 1393
SmoI CTYRAG 2 cut(s) 1337, 1393
SphI GCATGC 2 cut(s) 308, 1058
SsiI CCGC 4 cut(s) 838, 998, 1124, 1297
SspMI CTAG 1 cut(s) 392
StyD4I CCNGG 1 cut(s) 1242
StyI CCWWGG 3 cut(s) 116, 224, 519
TaaI ACNGT 7 cut(s) 425, 469, 596, 709, 1033, 1111, 1250
TaiI ACGT 3 cut(s) 643, 1071, 1406
TaqI TCGA 2 cut(s) 46, 479
TatI WGTACW 5 cut(s) 376, 415, 704, 1291, 1323
TauI GCSGC 1 cut(s) 1300
TfiI GAWTC 3 cut(s) 386, 660, 1172
Tru1I TTAA 5 cut(s) 845, 954, 1136, 1286, 1338
Tru9I TTAA 5 cut(s) 845, 954, 1136, 1286, 1338
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 636, 1225
TseI GCWGC 2 cut(s) 1100, 1304
Tsp45I GTSAC 2 cut(s) 636, 1225
TspDTI ATGAA 4 cut(s) 830, 873, 1215, 1344
TspGWI ACGGA 1 cut(s) 1329
TspRI CASTG 1 cut(s) 238
Vha464I CTTAAG 1 cut(s) 1337
VpaK11BI GGWCC 3 cut(s) 630, 810, 1280
XapI RAATTY 3 cut(s) 39, 860, 967
XceI RCATGY 3 cut(s) 308, 1058, 1082
XmiI GTMKAC 2 cut(s) 471, 488
XspI CTAG 1 cut(s) 392
ZrmI AGTACT 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.