Rh7DG156900

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3- polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin ferredoxin reductase system to COQ6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0011500
Physical Location & Seq
Reverse (-)
13564551 .. 13567843
3293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG156900.1

Sequence Viewer

Length: 1302 bp
ATGCCGATGACAAAGCACTTGAAGGTGGCCATCATTGATAGCAATCCCGCATTATCTAATGGACTCTCAATCAAGAAAGAAGACCCCCCTGATGCAAGGGTCAGTACAGTAACACCTGCAACCATATCTCTCTTCAAAGATATTGGCGCTTGGAAATATGTTGAACAGCATCGACATGCATATTTTGATCAAATGCAGGTTTGGGACTATACTGGCTTAGGATATACGAGATACGATGCTAGAGATGTACATAAAGAATATCTCGGGTGTGTAGTGGAGAATAAAGTGCTGCACAGTTCTCTTTTGTCATGCATGCAGGATACAGATTTCCAGAAGACTATTTACCCTTCCAGATTATCCTCAATGGCTTTAAATCCAGGCATTCTATCTATGGGGATGAACAGCACATCATCTGGGTTGAATGCGCGTGGAAACTTAGCAAAACTGGATCTAAGTGATGGCAATAGTTTATATGCAAAGTTGGTGGTTGGAGCTGATGGGTCCAAGTCACGTGTTAGGGAGTTGGCAGGATTTAAGACGACTGGATGGAAATACTCACAGAATGCAATCATTTGCACAGTAGAGCATCATGCAGAAAATCGATGTGCATGGCAAAGATTTCTACCTACCGGACCAATTGCACTTTTGCCTATTGGTGATAATTTTAGTAACATTGTTTGGACTATGAACCCGGAAGAAGCAACTGACCGTAAATCAATGGTCGAGGATGATTTTGTGAAAGCCGTAAATTCTGCTCTTGATTATGGATTTGGCCCTCATCCTAAGTCGAGCAACTTCGGAAGTGGAGGCATTTTTTCTTGGTTTAAGACAGACACAACTATTTCTGCCAATGAATGCTTCAAAGTTCCGCCGAAAGTGATGAAGTTGGCATCTGAAAGAATGGTGTTTCCCTTGTCTTTGATGCATGCCAATAACTATGTGTCAAAGCATGTGGTTCTAATTGGCGATGCAGCACACACTGTTCACCCTTTGGCTGGGCAAGGAGTTAATCTGGGTTTTGGAGATGCATTTGCTCTTTCAAGAATCATTTCTGAGGGCATTGCAGTGGGAACAGATATTTCTGAGGTCAATTTGTTGAAGAAATATGAAGCAGAGAGAAAAACGGCTAACGTTACAATGATGGCAATCCTAGATGGTTTCCAGAGGGCTTACTCAGTTGATTTTGGACCTTTAAATGTTCTACGCGCTGCTGCATTCCACGGAGCACAGTACATTCCACCTCTTAAAAGGAGTATAATCTCATATGCATCAGGGGATCAGAGATTGCCAATATTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01100 ko01110 map00130 map01100 map01110
Pfam Domains
Protein Families
DUF1232 FAD_binding_3

Protein Analysis

433

Amino Acids

47.58

Weight (kDa)

8.42

Isoelectric Point (pI)

23.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 33 - 253 1e-05 FAD binding domain
FAD_binding_3 PF01494 300 - 376 9.3e-12 FAD binding domain
SE PF08491 303 - 407 8.7e-08 Squalene epoxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24200 AT3G24200
fragaria_vesca FvH4_5g03680 FvH4_5g03680 FvH4_5g03680
malus_domestica MD06G1096300.v1.1 MD14G1116500.v1.1
prunus_persica Prupe.5G110900_v2.0.a1
pyrus_communis pycom06g09260
rosa_chinensis RchiOBHm_Chr7g0196051
rosa_laevigata RLG00000004070
rosa_multiflora Rmu_sc0001640.1_g000009
rosa_roxburghii Rroxscaffold_3G00259900
rosa_rugosa Rorug07G0029300 Rorug07G0029400
rosa_samantha Rh7AG156000 Rh7BG157700 Rh7CG161400 Rh7DG156900
rosa_wichuraiana Rw7G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 124
Acc36I ACCTGC 2 cut(s) 124, 187
AccII CGCG 2 cut(s) 427, 1206
AciI CCGC 2 cut(s) 48, 869
AclI AACGTT 1 cut(s) 1131
AclWI GGATC 2 cut(s) 456, 1284
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 748
AcvI CACGTG 1 cut(s) 512
AfaI GTAC 3 cut(s) 106, 249, 1232
AfiI CCNNNNNNNGG 1 cut(s) 995
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 7 cut(s) 22, 136, 164, 421, 862, 1041, 1099
AjnI CCWGG 1 cut(s) 376
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 1 cut(s) 494
AluI AGCT 1 cut(s) 494
Alw21I GWGCWC 1 cut(s) 1228
AlwI GGATC 2 cut(s) 456, 1284
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 2 cut(s) 27, 772
ApeKI GCWGC 4 cut(s) 289, 971, 1208, 1211
ApoI RAATTY 1 cut(s) 748
Asp700I GAANNNNTTC 1 cut(s) 1048
AspLEI GCGC 3 cut(s) 149, 427, 1208
AspS9I GGNCC 4 cut(s) 501, 632, 773, 1187
AsuC2I CCSGG 1 cut(s) 692
AsuHPI GGTGA 2 cut(s) 668, 977
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 3 cut(s) 501, 632, 1187
BalI TGGCCA 1 cut(s) 29
BarI GAAGNNNNNNTAC 2 cut(s) 326, 358
BbrPI CACGTG 1 cut(s) 512
BbsI GAAGAC 2 cut(s) 87, 341
Bbv12I GWGCWC 1 cut(s) 1228
BbvI GCAGC 4 cut(s) 276, 983, 1195, 1198
BccI CCATC 6 cut(s) 38, 452, 491, 540, 1135, 1148
BceAI ACGGC 2 cut(s) 728, 1140
BciT130I CCWGG 1 cut(s) 378
BciVI GTATCC 1 cut(s) 313
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 1 cut(s) 692
BfaI CTAG 2 cut(s) 240, 1151
BfoI RGCGCY 1 cut(s) 150
BfuAI ACCTGC 2 cut(s) 124, 187
BfuI GTATCC 1 cut(s) 313
BisI GCNGC 4 cut(s) 290, 972, 1209, 1212
BlsI GCNGC 4 cut(s) 291, 973, 1210, 1213
Bme1390I CCNGG 2 cut(s) 378, 692
Bme18I GGWCC 3 cut(s) 501, 632, 1187
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 4 cut(s) 501, 632, 773, 1187
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 2 cut(s) 378, 692
BmsI GCATC 9 cut(s) 82, 178, 226, 595, 899, 912, 958, 1015, 1277
BpiI GAAGAC 2 cut(s) 87, 341
Bpu10I CCTNAGC 1 cut(s) 217
BpuMI CCSGG 1 cut(s) 692
Bsa29I ATCGAT 1 cut(s) 601
BsaAI YACGTR 1 cut(s) 512
BsaBI GATNNNNATC 2 cut(s) 42, 1145
BsaJI CCNNGG 1 cut(s) 1219
BsaWI WCCGGW 1 cut(s) 629
Bsc4I CCNNNNNNNGG 1 cut(s) 995
Bse1I ACTGG 3 cut(s) 217, 450, 547
Bse3DI GCAATG 1 cut(s) 1059
Bse8I GATNNNNATC 2 cut(s) 42, 1145
BseBI CCWGG 1 cut(s) 378
BseCI ATCGAT 1 cut(s) 601
BseDI CCNNGG 1 cut(s) 1219
BseGI GGATG 4 cut(s) 402, 551, 733, 778
BseJI GATNNNNATC 2 cut(s) 42, 1145
BseLI CCNNNNNNNGG 1 cut(s) 995
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 3 cut(s) 1044, 1074, 1188
BseNI ACTGG 3 cut(s) 217, 450, 547
BseXI GCAGC 4 cut(s) 276, 983, 1195, 1198
BseYI CCCAGC 1 cut(s) 995
BsgI GTGCAG 1 cut(s) 275
Bsh1236I CGCG 2 cut(s) 427, 1206
BshFI GGCC 2 cut(s) 29, 774
BshVI ATCGAT 1 cut(s) 601
BsiHKAI GWGCWC 1 cut(s) 1228
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 2 cut(s) 630, 692
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 995
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 5 cut(s) 381, 427, 568, 860, 1214
BsnI GGCC 2 cut(s) 29, 774
BsoBI CYCGRG 1 cut(s) 263
Bsp1286I GDGCHC 1 cut(s) 1228
Bsp1407I TGTACA 1 cut(s) 247
Bsp143I GATC 3 cut(s) 187, 448, 1276
BspACI CCGC 2 cut(s) 48, 869
BspANI GGCC 2 cut(s) 29, 774
BspCNI CTCAG 3 cut(s) 1045, 1075, 1187
BspDI ATCGAT 1 cut(s) 601
BspFNI CGCG 2 cut(s) 427, 1206
BspLI GGNNCC 1 cut(s) 502
BspMI ACCTGC 2 cut(s) 124, 187
BspPI GGATC 2 cut(s) 456, 1284
BsrDI GCAATG 1 cut(s) 1059
BsrGI TGTACA 1 cut(s) 247
BsrI ACTGG 3 cut(s) 217, 450, 547
BssECI CCNNGG 1 cut(s) 1219
BssMI GATC 3 cut(s) 187, 448, 1276
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 6 cut(s) 109, 296, 580, 710, 982, 1230
Bst6I CTCTTC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 247
BstBAI YACGTR 1 cut(s) 512
BstC8I GCNNGC 2 cut(s) 314, 927
BstDEI CTNAG 7 cut(s) 217, 436, 452, 783, 1053, 1083, 1174
BstDSI CCRYGG 1 cut(s) 1219
BstF5I GGATG 4 cut(s) 402, 551, 733, 778
BstFNI CGCG 2 cut(s) 427, 1206
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 3 cut(s) 149, 427, 1208
BstKTI GATC 3 cut(s) 190, 451, 1279
BstMBI GATC 3 cut(s) 187, 448, 1276
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 4 cut(s) 179, 316, 929, 953
BstSCI CCNGG 2 cut(s) 376, 690
BstUI CGCG 2 cut(s) 427, 1206
BstV1I GCAGC 4 cut(s) 276, 983, 1195, 1198
BstV2I GAAGAC 2 cut(s) 87, 341
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
Bsu15I ATCGAT 1 cut(s) 601
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 2 cut(s) 29, 774
BsuTUI ATCGAT 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 1219
BtgZI GCGATG 1 cut(s) 981
BtsCI GGATG 4 cut(s) 402, 551, 733, 778
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 2 cut(s) 978, 1071
BveI ACCTGC 2 cut(s) 124, 187
Cac8I GCNNGC 2 cut(s) 314, 927
CfoI GCGC 3 cut(s) 149, 427, 1208
Cfr13I GGNCC 4 cut(s) 501, 632, 773, 1187
ClaI ATCGAT 1 cut(s) 601
Csp6I GTAC 3 cut(s) 105, 248, 1231
CspCI CAANNNNNGTGG 4 cut(s) 465, 500, 933, 968
CviAII CATG 7 cut(s) 176, 309, 313, 590, 609, 926, 950
CviJI RGCY 9 cut(s) 29, 216, 368, 494, 743, 774, 995, 1127, 1169
CviKI_1 RGCY 9 cut(s) 29, 216, 368, 494, 743, 774, 995, 1127, 1169
CviQI GTAC 3 cut(s) 105, 248, 1231
DdeI CTNAG 7 cut(s) 217, 436, 452, 783, 1053, 1083, 1174
DpnI GATC 3 cut(s) 189, 450, 1278
DpnII GATC 3 cut(s) 187, 448, 1276
DraI TTTAAA 2 cut(s) 372, 1194
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EciI GGCGGA 1 cut(s) 858
Eco47I GGWCC 3 cut(s) 501, 632, 1187
Eco72I CACGTG 1 cut(s) 512
Eco88I CYCGRG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 376
EcoT22I ATGCAT 5 cut(s) 181, 314, 927, 1030, 1270
FaeI CATG 7 cut(s) 179, 312, 316, 593, 612, 929, 953
FaqI GGGAC 1 cut(s) 218
FatI CATG 7 cut(s) 175, 308, 312, 589, 608, 925, 949
FauI CCCGC 1 cut(s) 55
FauNDI CATATG 1 cut(s) 1264
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 4 cut(s) 290, 972, 1209, 1212
FokI GGATG 4 cut(s) 409, 558, 740, 765
Fsp4HI GCNGC 4 cut(s) 290, 972, 1209, 1212
FspBI CTAG 2 cut(s) 240, 1151
GlaI GCGC 3 cut(s) 148, 426, 1207
GluI GCNGC 4 cut(s) 290, 972, 1209, 1212
GsaI CCCAGC 1 cut(s) 999
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 29, 774
HapII CCGG 2 cut(s) 630, 692
HhaI GCGC 3 cut(s) 149, 427, 1208
Hin1II CATG 7 cut(s) 179, 312, 316, 593, 612, 929, 953
Hin6I GCGC 3 cut(s) 147, 425, 1206
HinP1I GCGC 3 cut(s) 147, 425, 1206
HinfI GANTC 2 cut(s) 63, 1044
HpaII CCGG 2 cut(s) 630, 692
HphI GGTGA 2 cut(s) 668, 977
Hpy166II GTNNAC 1 cut(s) 985
Hpy188I TCNGA 5 cut(s) 800, 895, 1054, 1084, 1281
Hpy188III TCNNGA 7 cut(s) 73, 331, 351, 758, 1041, 1162, 1299
Hpy8I GTNNAC 1 cut(s) 985
HpyAV CCTTC 2 cut(s) 16, 357
HpyCH4III ACNGT 6 cut(s) 109, 296, 580, 710, 982, 1230
HpyCH4IV ACGT 2 cut(s) 511, 1131
HpyF3I CTNAG 7 cut(s) 217, 436, 452, 783, 1053, 1083, 1174
HpySE526I ACGT 2 cut(s) 511, 1131
Hsp92II CATG 7 cut(s) 179, 312, 316, 593, 612, 929, 953
HspAI GCGC 3 cut(s) 147, 425, 1206
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 3 cut(s) 187, 448, 1276
LmnI GCTCC 2 cut(s) 491, 1223
Lsp1109I GCAGC 4 cut(s) 276, 983, 1195, 1198
LweI GCATC 9 cut(s) 82, 178, 226, 595, 899, 912, 958, 1015, 1277
MaeI CTAG 2 cut(s) 240, 1151
MaeII ACGT 2 cut(s) 511, 1131
MaeIII GTNAC 4 cut(s) 109, 507, 668, 1132
MalI GATC 3 cut(s) 189, 450, 1278
MboI GATC 3 cut(s) 187, 448, 1276
MboII GAAGA 5 cut(s) 92, 124, 346, 707, 1111
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 1 cut(s) 1228
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 5 cut(s) 636, 661, 748, 960, 1090
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 1 cut(s) 57
MmeI TCCRAC 1 cut(s) 469
MnlI CCTC 8 cut(s) 370, 718, 786, 800, 1048, 1078, 1158, 1251
Mox20I TGGCCA 1 cut(s) 29
Mph1103I ATGCAT 5 cut(s) 181, 314, 927, 1030, 1270
MroXI GAANNNNTTC 1 cut(s) 1048
MscI TGGCCA 1 cut(s) 29
MseI TTAA 6 cut(s) 371, 534, 825, 1008, 1193, 1245
MslI CAYNNNNRTG 2 cut(s) 174, 1064
Msp20I TGGCCA 1 cut(s) 29
MspI CCGG 2 cut(s) 630, 692
MspR9I CCNGG 2 cut(s) 378, 692
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 5 cut(s) 381, 427, 568, 860, 1214
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 427, 1206
NciI CCSGG 1 cut(s) 692
NdeI CATATG 1 cut(s) 1264
NdeII GATC 3 cut(s) 187, 448, 1276
NlaIII CATG 7 cut(s) 179, 312, 316, 593, 612, 929, 953
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 507
NsiI ATGCAT 5 cut(s) 181, 314, 927, 1030, 1270
NspI RCATGY 4 cut(s) 179, 316, 929, 953
PaeI GCATGC 2 cut(s) 316, 929
PaqCI CACCTGC 1 cut(s) 124
PctI GAATGC 5 cut(s) 381, 427, 568, 860, 1214
PdmI GAANNNNTTC 1 cut(s) 1048
PfeI GAWTC 1 cut(s) 1044
PkrI GCNGC 4 cut(s) 291, 973, 1210, 1213
PleI GAGTC 1 cut(s) 57
PmaCI CACGTG 1 cut(s) 512
PmlI CACGTG 1 cut(s) 512
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 512
Psp1406I AACGTT 1 cut(s) 1131
Psp6I CCWGG 1 cut(s) 376
PspCI CACGTG 1 cut(s) 512
PspFI CCCAGC 1 cut(s) 995
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 502
PspPI GGNCC 4 cut(s) 501, 632, 773, 1187
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 3 cut(s) 106, 249, 1232
RsaNI GTAC 3 cut(s) 105, 248, 1231
RseI CAYNNNNRTG 2 cut(s) 174, 1064
SaqAI TTAA 6 cut(s) 371, 534, 825, 1008, 1193, 1245
SatI GCNGC 4 cut(s) 290, 972, 1209, 1212
Sau3AI GATC 3 cut(s) 187, 448, 1276
Sau96I GGNCC 4 cut(s) 501, 632, 773, 1187
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 2 cut(s) 378, 692
SduI GDGCHC 1 cut(s) 1228
SfaNI GCATC 9 cut(s) 82, 178, 226, 595, 899, 912, 958, 1015, 1277
SinI GGWCC 3 cut(s) 501, 632, 1187
SmiMI CAYNNNNRTG 2 cut(s) 174, 1064
SphI GCATGC 2 cut(s) 316, 929
Sse9I AATT 5 cut(s) 636, 661, 748, 960, 1090
SsiI CCGC 2 cut(s) 48, 869
SspI AATATT 1 cut(s) 1293
SspMI CTAG 2 cut(s) 240, 1151
StyD4I CCNGG 2 cut(s) 376, 690
TaaI ACNGT 6 cut(s) 109, 296, 580, 710, 982, 1230
TaiI ACGT 2 cut(s) 514, 1134
TaqI TCGA 4 cut(s) 172, 601, 723, 788
TasI AATT 5 cut(s) 636, 661, 748, 960, 1090
TatI WGTACW 3 cut(s) 104, 247, 1230
TfiI GAWTC 1 cut(s) 1044
Tru1I TTAA 6 cut(s) 371, 534, 825, 1008, 1193, 1245
Tru9I TTAA 6 cut(s) 371, 534, 825, 1008, 1193, 1245
TscAI CASTG 2 cut(s) 985, 1071
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 4 cut(s) 289, 971, 1208, 1211
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 5 cut(s) 413, 701, 867, 896, 1122
TspGWI ACGGA 1 cut(s) 1236
TspRI CASTG 2 cut(s) 985, 1071
VpaK11BI GGWCC 3 cut(s) 501, 632, 1187
XapI RAATTY 1 cut(s) 748
XceI RCATGY 4 cut(s) 179, 316, 929, 953
XmnI GAANNNNTTC 1 cut(s) 1048
XspI CTAG 2 cut(s) 240, 1151
Zsp2I ATGCAT 5 cut(s) 181, 314, 927, 1030, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.