AT3G25210

Pentatricopeptide repeat-containing protein At3g25210

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013547
Physical Location & Seq
Forward (+)
9180244 .. 9181955
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G25210.1

Sequence Viewer

Length: 1140 bp
ATGTCGGCGACACTCCGACGCCTCATTCTTCTCACCTCCACAAATCGCTCCCGTAATCTATCTCCGCCGTTTCTTTCCACCGCCGTGAACCACCTATCTCTATCATTCTCTTCCGTCTCATCTTCACCGGAATCACACACTTCCCCTTCCCGGATCCGAACCCGAACACCACTCGAAACTCAATTCGAAACATGGATTCAGAATCTAAAACCAGGATTCACAAACTCCGACGTTGTGATAGCGTTACGAGCTCAATCCGATCCAGATCTAGCTCTCGACATATTCCGATGGACAGCTCAACAACGAGGCTACAAACACAATCACGAAGCTTACCACACTATGATCAAACAAGCAATCACCGGAAAACGAAACAACTTCGTCGAAACGTTAATCGAAGAAGTAATCGCCGGAGCTTGTGAAATGAGTGTTCCTCTCTACAATTGCATCATCAGATTCTGTTGTGGTCGTAAGTTTCTCTTCAATAGAGCTTTCGATGTTTATAACAAAATGCTTCGATCAGATGATTCAAAGCCTGACCTTGAAACTTATACTTTGCTACTAAGTTCATTGCTTAAGAGATTCAACAAATTGAATGTGTGTTATGTGTATCTACACGCCGTTAGATCGTTAACGAAGCAGATGAAATCGAATGGTGTGATTCCGGATACGTTTGTGTTGAATATGATTATCAAAGCTTATGCAAAGTGTCTTGAGGTTGATGAAGCTATAAGAGTGTTTAAAGAGATGGCTTTATATGGCTCTGAGCCTAATGCTTATACTTATAGTTACTTAGTTAAAGGGGTTTGTGAGAAAGGTAGAGTTGGACAGGGTTTAGGGTTTTATAAGGAGATGCAAGTCAAAGGAATGGTTCCGAATGGGAGTTGTTATATGGTTTTGATTTGTAGTTTGTCTATGGAGAGGAGATTGGATGAAGCTGTTGAGGTTGTGTATGATATGTTGGCGAATTCGTTGTCTCCGGATATGTTGACGTATAATACGGTGTTGACGGAGTTGTGTAGGGGAGGTAGAGGGAGTGAAGCTCTTGAGATGGTTGAGGAATGGAAGAAACGGGATCCGGTGATGGGTGAAAGGAATTACAGAACGTTGATGGATGAGGTTTATTTTTTGAATAAGGGATGA

Protein Analysis

379

Amino Acids

43.25

Weight (kDa)

8.78

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 143 - 191 1.5e-06 PPR repeat family
PPR_long PF17177 154 - 277 2.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 217 - 249 4.5e-06 PPR repeat
PPR_2 PF13041 221 - 269 2e-13 PPR repeat family
PPR_2 PF13041 295 - 340 7e-08 PPR repeat family
PPR_1 PF12854 323 - 355 1.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25210
fragaria_vesca FvH4_3g25580
malus_domestica MD05G1166500.v1.1 MD10G1156500.v1.1
prunus_persica Prupe.8G194300_v2.0.a1
pyrus_communis pycom05g15510 pycom10g13470
rosa_chinensis RchiOBHm_Chr5g0046411
rosa_laevigata RLG00000034408
rosa_multiflora Rmu_sc0003878.1_g000050
rosa_roxburghii Rroxscaffold_1G00034990 Rroxscaffold_7G00197530
rosa_rugosa Rorug05G0227800
rosa_samantha Rh5AG309000 Rh5BG317200 Rh5CG343100 Rh5DG327400
rosa_wichuraiana Rw0G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 501, 843
AccIII TCCGGA 2 cut(s) 661, 976
AciI CCGC 2 cut(s) 65, 81
AclI AACGTT 2 cut(s) 386, 1103
AclWI GGATC 5 cut(s) 148, 161, 254, 1067, 1080
AcsI RAATTY 1 cut(s) 964
AcyI GRCGYC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 150, 1082
AflII CTTAAG 1 cut(s) 572
AgsI TTSAA 7 cut(s) 481, 528, 542, 583, 592, 679, 1129
AjnI CCWGG 1 cut(s) 211
AleI CACNNNNGTG 1 cut(s) 83
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 2 cut(s) 121, 978
AlwI GGATC 5 cut(s) 148, 161, 254, 1067, 1080
AlwNI CAGNNNCTG 1 cut(s) 456
Aor13HI TCCGGA 2 cut(s) 661, 976
ApoI RAATTY 1 cut(s) 964
ArsI GACNNNNNNTTYG 2 cut(s) 956, 988
AsuC2I CCSGG 1 cut(s) 151
AsuHPI GGTGA 5 cut(s) 25, 117, 349, 1090, 1097
AsuII TTCGAA 1 cut(s) 186
BaeI ACNNNNGTAYC 2 cut(s) 657, 690
BamHI GGATCC 2 cut(s) 153, 1072
BanII GRGCYC 1 cut(s) 253
Bbv12I GWGCWC 1 cut(s) 253
BccI CCATC 5 cut(s) 282, 739, 1042, 1075, 1102
BceAI ACGGC 3 cut(s) 52, 68, 602
BciT130I CCWGG 1 cut(s) 213
BciVI GTATCC 1 cut(s) 658
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 121, 978
BfaI CTAG 1 cut(s) 269
BfrI CTTAAG 1 cut(s) 572
BfuI GTATCC 1 cut(s) 658
BglII AGATCT 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 151, 213
BmiI GGNNCC 3 cut(s) 155, 870, 1074
BmrFI CCNGG 2 cut(s) 151, 213
BmsI GCATC 2 cut(s) 453, 840
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 1024, 1056
Bpu14I TTCGAA 1 cut(s) 186
BpuEI CTTGAG 2 cut(s) 731, 1064
BpuMI CCSGG 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 19
BsaWI WCCGGW 5 cut(s) 127, 359, 661, 976, 1075
Bsc4I CCNNNNNNNGG 2 cut(s) 150, 1082
Bse3DI GCAATG 1 cut(s) 566
Bse8I GATNNNNATC 1 cut(s) 264
BseAI TCCGGA 2 cut(s) 661, 976
BseBI CCWGG 1 cut(s) 213
BseGI GGATG 2 cut(s) 934, 1117
BseJI GATNNNNATC 1 cut(s) 264
BseLI CCNNNNNNNGG 2 cut(s) 150, 1082
BseMI GCAATG 1 cut(s) 566
BseMII CTCAG 1 cut(s) 753
BseRI GAGGAG 1 cut(s) 934
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 7 cut(s) 128, 151, 360, 408, 662, 977, 1076
BslI CCNNNNNNNGG 2 cut(s) 150, 1082
BsmAI GTCTC 2 cut(s) 121, 978
BsmBI CGTCTC 1 cut(s) 121
Bsp119I TTCGAA 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 253
Bsp13I TCCGGA 2 cut(s) 661, 976
Bsp143I GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
BspACI CCGC 2 cut(s) 65, 81
BspCNI CTCAG 1 cut(s) 754
BspEI TCCGGA 2 cut(s) 661, 976
BspLI GGNNCC 3 cut(s) 155, 870, 1074
BspPI GGATC 5 cut(s) 148, 161, 254, 1067, 1080
BspT104I TTCGAA 1 cut(s) 186
BspTI CTTAAG 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 566
BssMI GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
BssNI GRCGYC 1 cut(s) 19
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 1000
Bst6I CTCTTC 2 cut(s) 115, 482
BstACI GRCGYC 1 cut(s) 19
BstAFI CTTAAG 1 cut(s) 572
BstBI TTCGAA 1 cut(s) 186
BstDEI CTNAG 3 cut(s) 560, 762, 790
BstF5I GGATG 2 cut(s) 934, 1117
BstKTI GATC 7 cut(s) 156, 262, 268, 345, 518, 626, 1075
BstMAI GTCTC 2 cut(s) 121, 978
BstMBI GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 2 cut(s) 149, 211
BstX2I RGATCY 3 cut(s) 153, 265, 1072
BstYI RGATCY 3 cut(s) 153, 265, 1072
BsuI GTATCC 1 cut(s) 658
BtsCI GGATG 2 cut(s) 934, 1117
CaiI CAGNNNCTG 1 cut(s) 456
CseI GACGC 1 cut(s) 27
CviAII CATG 1 cut(s) 192
DdeI CTNAG 3 cut(s) 560, 762, 790
DpnI GATC 7 cut(s) 155, 261, 267, 344, 517, 625, 1074
DpnII GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
DraI TTTAAA 1 cut(s) 739
Eam1104I CTCTTC 2 cut(s) 115, 482
EarI CTCTTC 2 cut(s) 115, 482
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 253
Eco53kI GAGCTC 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 251
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 1 cut(s) 211
EcoT38I GRGCYC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 1 cut(s) 195
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FatI CATG 1 cut(s) 191
FbaI TGATCA 1 cut(s) 342
FokI GGATG 2 cut(s) 941, 1124
FriOI GRGCYC 1 cut(s) 253
FspBI CTAG 1 cut(s) 269
HapII CCGG 7 cut(s) 128, 151, 360, 408, 662, 977, 1076
HgaI GACGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 1 cut(s) 195
HincII GTYRAC 3 cut(s) 630, 987, 1005
HindII GTYRAC 3 cut(s) 630, 987, 1005
HindIII AAGCTT 2 cut(s) 327, 693
HinfI GANTC 8 cut(s) 131, 196, 202, 216, 453, 524, 579, 658
HpaI GTTAAC 1 cut(s) 630
HpaII CCGG 7 cut(s) 128, 151, 360, 408, 662, 977, 1076
HphI GGTGA 5 cut(s) 25, 117, 349, 1090, 1097
Hpy166II GTNNAC 4 cut(s) 88, 630, 987, 1005
Hpy188III TCNNGA 7 cut(s) 263, 275, 323, 662, 710, 977, 1043
Hpy8I GTNNAC 4 cut(s) 88, 630, 987, 1005
Hpy99I CGWCG 3 cut(s) 21, 233, 383
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 1 cut(s) 1000
HpyCH4IV ACGT 5 cut(s) 231, 386, 668, 989, 1103
HpyCH4V TGCA 3 cut(s) 444, 701, 853
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 3 cut(s) 560, 762, 790
HpySE526I ACGT 5 cut(s) 231, 386, 668, 989, 1103
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 1 cut(s) 195
Kpn2I TCCGGA 2 cut(s) 661, 976
Ksp22I TGATCA 1 cut(s) 342
KspAI GTTAAC 1 cut(s) 630
Kzo9I GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
LmnI GCTCC 2 cut(s) 53, 410
LweI GCATC 2 cut(s) 453, 840
MaeI CTAG 1 cut(s) 269
MaeII ACGT 5 cut(s) 231, 386, 668, 989, 1103
MaeIII GTNAC 2 cut(s) 243, 785
MalI GATC 7 cut(s) 155, 261, 267, 344, 517, 625, 1074
MboI GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
MboII GAAGA 6 cut(s) 20, 102, 114, 407, 469, 1075
MfeI CAATTG 1 cut(s) 439
MflI RGATCY 3 cut(s) 153, 265, 1072
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 5 cut(s) 182, 439, 587, 964, 1093
MmeI TCCRAC 3 cut(s) 40, 252, 802
MroI TCCGGA 2 cut(s) 661, 976
MseI TTAA 5 cut(s) 389, 573, 629, 738, 795
MslI CAYNNNNRTG 1 cut(s) 83
MspCI CTTAAG 1 cut(s) 572
MspI CCGG 7 cut(s) 128, 151, 360, 408, 662, 977, 1076
MspR9I CCNGG 2 cut(s) 151, 213
MunI CAATTG 1 cut(s) 439
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 1 cut(s) 248
NciI CCSGG 1 cut(s) 151
NdeII GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
NlaIII CATG 1 cut(s) 195
NlaIV GGNNCC 3 cut(s) 155, 870, 1074
NspV TTCGAA 1 cut(s) 186
OliI CACNNNNGTG 1 cut(s) 83
PfeI GAWTC 8 cut(s) 131, 196, 202, 216, 453, 524, 579, 658
PfoI TCCNGGA 1 cut(s) 149
PsiI TTATAA 2 cut(s) 501, 843
Psp124BI GAGCTC 1 cut(s) 253
Psp1406I AACGTT 2 cut(s) 386, 1103
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 3 cut(s) 155, 870, 1074
PstNI CAGNNNCTG 1 cut(s) 456
PsuI RGATCY 3 cut(s) 153, 265, 1072
RseI CAYNNNNRTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 5 cut(s) 389, 573, 629, 738, 795
Sau3AI GATC 7 cut(s) 153, 259, 265, 342, 515, 623, 1072
ScrFI CCNGG 2 cut(s) 151, 213
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 2 cut(s) 453, 840
SfuI TTCGAA 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 572, 710, 1043
SmoI CTYRAG 3 cut(s) 572, 710, 1043
Sse9I AATT 5 cut(s) 182, 439, 587, 964, 1093
SsiI CCGC 2 cut(s) 65, 81
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 2 cut(s) 149, 211
TaaI ACNGT 1 cut(s) 1000
TaiI ACGT 5 cut(s) 234, 389, 671, 992, 1106
TaqI TCGA 8 cut(s) 174, 186, 276, 381, 393, 492, 514, 647
TasI AATT 5 cut(s) 182, 439, 587, 964, 1093
TfiI GAWTC 8 cut(s) 131, 196, 202, 216, 453, 524, 579, 658
Tru1I TTAA 5 cut(s) 389, 573, 629, 738, 795
Tru9I TTAA 5 cut(s) 389, 573, 629, 738, 795
TspDTI ATGAA 4 cut(s) 555, 656, 735, 945
TspGWI ACGGA 2 cut(s) 103, 1022
Vha464I CTTAAG 1 cut(s) 572
XapI RAATTY 1 cut(s) 964
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.