MD10G1156500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013547
Physical Location & Seq
Reverse (-)
24499192 .. 24500379
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1156500.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGCCGGTGACCTTACGCCGCCTTCTCACCGGCGTCACCGCCACTATCAGAAACCCTAATGTTCACCTTCTTCCACGCCCACCTCTCCCCTATCGCCACGTACATCCCTACCCCTCATTTCCGTCCTCACATCCGTCACTCTATCTCAACCACCGCCTCCTGTCCTCCTCACCCACAGAAAACCCTATCCCCCCAAACCCGCTCCGAACCCGAACCCCACTTGAAAAGCAATTCGAGACGTGGGTCCAGAAGCTCAAACCGGGGTTCGGGCCGCTCGAAGTGAACGAGGCCCTGAAAGCCCAATCGGACGCGGATCTCGGCCTCGACATTTTCCGATGGACCGCCCAGCAGCGGAACTACAAGCACAACCACGCCACCTACCTCACCATGATCAGAATCTTGCTCGACGGCAGACGCTACCGCCACGCCGAAACCCTAGTCGAAGAGGTAATCGCCGGAGCCTGTGAAATTAGCGTGCCCCTTTACAATTCCATGATTCGATTTTGCTGCGGAAGAAAGCTGCTTTTCAATCGTGCTTTTGATATTTATAAGAAAATGTTGAATTCCGAGAATTGCAAACCGAATCTTGAAACTTATGCATTGCTGCTGAACTCGTTGCTTAGGAGGTTTAATAATATGCATGTTTGCTATGTGTACTTGCGCGCCGTGCGGTCTTTGTCGAAGCAGATGAAGGCGTGCGGTGTGATTCCGGATACGTTTGTGTTGAATATGATCATAAAGGCGTATTCGAAGTGTTTGCAGGTTGATGATGCAATGCAGGTTTTTCGCGAAATGGGGTTGTACGGGTGTGAGCCAAATGCGTATACTTATGGCTACATAGCTAAGGGGTTGTGTGAGAAGGGGAGGGTTGCTCAGGGTTTAGGGTTTTACAAGGAAATGAGAGGAAAAGGGTTGGTGCCTAGCAGTAGTACGTATATGATTGTGATTTGCAGTCTCGCTATGGGACGGAAGTATGATGATGCAACTGAGGTTGTGCTTGATATGTTGAGTAATGCTATGTCTCCTGATTTGTTAACTTACAAAACGTTGTTGGAAGGGTTGTGCCGGGATGGAAAGGGTGTCGAGGCATTTGATTTACTAGAGGATTTCCGGAGGAAGGATAGTATGATGAATGAAAGGACTTACAAGACTTTGTTAAATGCTTTGCATTTTGTAAATGGAGAGTAG

Protein Analysis

396

Amino Acids

45.17

Weight (kDa)

9.22

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 159 - 207 4.7e-07 PPR repeat family
PPR_long PF17177 170 - 292 3.4e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 237 - 285 1.4e-13 PPR repeat family
PPR_2 PF13041 282 - 320 5.3e-06 PPR repeat family
PPR_2 PF13041 312 - 356 2.5e-06 PPR repeat family
PPR_1 PF12854 340 - 368 5.5e-07 PPR repeat
PPR_2 PF13041 342 - 389 2.3e-09 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25210
fragaria_vesca FvH4_3g25580
malus_domestica MD05G1166500.v1.1 MD10G1156500.v1.1
prunus_persica Prupe.8G194300_v2.0.a1
pyrus_communis pycom05g15510 pycom10g13470
rosa_chinensis RchiOBHm_Chr5g0046411
rosa_laevigata RLG00000034408
rosa_multiflora Rmu_sc0003878.1_g000050
rosa_roxburghii Rroxscaffold_1G00034990 Rroxscaffold_7G00197530
rosa_rugosa Rorug05G0227800
rosa_samantha Rh5AG309000 Rh5BG317200 Rh5CG343100 Rh5DG327400
rosa_wichuraiana Rw0G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
Acc36I ACCTGC 2 cut(s) 751, 769
AccB1I GGYRCC 1 cut(s) 916
AccBSI CCGCTC 2 cut(s) 202, 274
AccI GTMKAC 1 cut(s) 824
AccII CGCG 3 cut(s) 311, 663, 789
AccIII TCCGGA 2 cut(s) 709, 1110
AclI AACGTT 1 cut(s) 1046
AclWI GGATC 1 cut(s) 321
AcsI RAATTY 1 cut(s) 562
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 4 cut(s) 102, 656, 803, 931
AfiI CCNNNNNNNGG 3 cut(s) 266, 351, 1117
AgsI TTSAA 5 cut(s) 224, 529, 562, 590, 727
AjiI CACGTC 1 cut(s) 240
AluBI AGCT 3 cut(s) 253, 520, 842
AluI AGCT 3 cut(s) 253, 520, 842
Alw26I GTCTC 3 cut(s) 230, 959, 1026
AlwI GGATC 1 cut(s) 321
Aor13HI TCCGGA 2 cut(s) 709, 1110
AoxI GGCC 3 cut(s) 269, 288, 319
ApeKI GCWGC 4 cut(s) 349, 507, 520, 604
ApoI RAATTY 1 cut(s) 562
ArsI GACNNNNNNTTYG 2 cut(s) 423, 455
AspLEI GCGC 2 cut(s) 663, 665
AspS9I GGNCC 4 cut(s) 244, 269, 289, 339
AsuC2I CCSGG 2 cut(s) 261, 1067
AsuHPI GGTGA 6 cut(s) 19, 19, 28, 56, 162, 376
AsuII TTCGAA 1 cut(s) 749
AvaII GGWCC 2 cut(s) 244, 339
BaeGI GKGCMC 1 cut(s) 480
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
BanI GGYRCC 1 cut(s) 916
BbvI GCAGC 4 cut(s) 361, 494, 507, 591
BccI CCATC 2 cut(s) 330, 1064
BceAI ACGGC 2 cut(s) 424, 650
BcgI CGANNNNNNTGC 6 cut(s) 489, 523, 739, 767, 773, 801
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 2 cut(s) 390, 732
BcnI CCSGG 2 cut(s) 261, 1067
BcoDI GTCTC 3 cut(s) 230, 959, 1026
BfaI CTAG 3 cut(s) 437, 921, 1100
BfuAI ACCTGC 2 cut(s) 751, 769
BfuI GTATCC 1 cut(s) 706
BisI GCNGC 6 cut(s) 19, 272, 350, 508, 521, 605
BlsI GCNGC 6 cut(s) 20, 273, 351, 509, 522, 606
Bme1390I CCNGG 2 cut(s) 261, 1067
Bme18I GGWCC 2 cut(s) 244, 339
BmgBI CACGTC 1 cut(s) 240
BmgT120I GGNCC 4 cut(s) 244, 269, 289, 339
BmiI GGNNCC 3 cut(s) 245, 460, 918
BmrFI CCNGG 2 cut(s) 261, 1067
BmsI GCATC 2 cut(s) 760, 970
BoxI GACNNNNGTC 1 cut(s) 242
BplI GAGNNNNNCTC 2 cut(s) 856, 888
Bpu10I CCTNAGC 3 cut(s) 620, 843, 873
Bpu14I TTCGAA 1 cut(s) 749
BpuMI CCSGG 2 cut(s) 261, 1067
BsaAI YACGTR 2 cut(s) 100, 933
BsaBI GATNNNNATC 1 cut(s) 395
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 260
BsaWI WCCGGW 2 cut(s) 709, 1110
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 351, 1117
Bse118I RCCGGY 2 cut(s) 4, 29
Bse3DI GCAATG 2 cut(s) 599, 780
Bse8I GATNNNNATC 1 cut(s) 395
BseAI TCCGGA 2 cut(s) 709, 1110
BseDI CCNNGG 1 cut(s) 260
BseGI GGATG 3 cut(s) 103, 130, 1075
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 3 cut(s) 266, 351, 1117
BseMI GCAATG 2 cut(s) 599, 780
BseMII CTCAG 2 cut(s) 887, 978
BsePI GCGCGC 1 cut(s) 661
BseRI GAGGAG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 4 cut(s) 361, 494, 507, 591
BseYI CCCAGC 1 cut(s) 345
Bsh1236I CGCG 3 cut(s) 311, 663, 789
BshFI GGCC 3 cut(s) 271, 290, 321
BshNI GGYRCC 1 cut(s) 916
BsiSI CCGG 7 cut(s) 5, 30, 260, 456, 710, 1066, 1111
BslFI GGGAC 1 cut(s) 978
BslI CCNNNNNNNGG 3 cut(s) 266, 351, 1117
BsmAI GTCTC 3 cut(s) 230, 959, 1026
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 978
BsnI GGCC 3 cut(s) 271, 290, 321
Bsp119I TTCGAA 1 cut(s) 749
Bsp1286I GDGCHC 1 cut(s) 480
Bsp13I TCCGGA 2 cut(s) 709, 1110
Bsp143I GATC 3 cut(s) 313, 390, 732
Bsp68I TCGCGA 1 cut(s) 789
BspANI GGCC 3 cut(s) 271, 290, 321
BspCNI CTCAG 2 cut(s) 886, 979
BspEI TCCGGA 2 cut(s) 709, 1110
BspFNI CGCG 3 cut(s) 311, 663, 789
BspLI GGNNCC 3 cut(s) 245, 460, 918
BspMI ACCTGC 2 cut(s) 751, 769
BspPI GGATC 1 cut(s) 321
BspT104I TTCGAA 1 cut(s) 749
BspT107I GGYRCC 1 cut(s) 916
BsrBI CCGCTC 2 cut(s) 202, 274
BsrDI GCAATG 2 cut(s) 599, 780
BsrFI RCCGGY 2 cut(s) 4, 29
BssAI RCCGGY 2 cut(s) 4, 29
BssECI CCNNGG 1 cut(s) 260
BssHII GCGCGC 1 cut(s) 661
BssMI GATC 3 cut(s) 313, 390, 732
BssNAI GTATAC 1 cut(s) 825
BssNI GRCGYC 1 cut(s) 33
Bst1107I GTATAC 1 cut(s) 825
Bst6I CTCTTC 1 cut(s) 438
BstACI GRCGYC 1 cut(s) 33
BstBAI YACGTR 2 cut(s) 100, 933
BstBI TTCGAA 1 cut(s) 749
BstC8I GCNNGC 3 cut(s) 476, 663, 697
BstDEI CTNAG 4 cut(s) 620, 843, 873, 987
BstEII GGTNACC 1 cut(s) 7
BstF5I GGATG 3 cut(s) 103, 130, 1075
BstFNI CGCG 3 cut(s) 311, 663, 789
BstHHI GCGC 2 cut(s) 663, 665
BstKTI GATC 3 cut(s) 316, 393, 735
BstMAI GTCTC 3 cut(s) 230, 959, 1026
BstMBI GATC 3 cut(s) 313, 390, 732
BstMWI GCNNNNNNNGC 2 cut(s) 296, 667
BstNSI RCATGY 1 cut(s) 644
BstPAI GACNNNNGTC 1 cut(s) 242
BstPI GGTNACC 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 259, 1065
BstSLI GKGCMC 1 cut(s) 480
BstSNI TACGTA 1 cut(s) 933
BstUI CGCG 3 cut(s) 311, 663, 789
BstV1I GCAGC 4 cut(s) 361, 494, 507, 591
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 825
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 3 cut(s) 271, 290, 321
BtrI CACGTC 1 cut(s) 240
BtsCI GGATG 3 cut(s) 103, 130, 1075
BtuMI TCGCGA 1 cut(s) 789
BveI ACCTGC 2 cut(s) 751, 769
Cac8I GCNNGC 3 cut(s) 476, 663, 697
CfoI GCGC 2 cut(s) 663, 665
Cfr10I RCCGGY 2 cut(s) 4, 29
Cfr13I GGNCC 4 cut(s) 244, 269, 289, 339
CseI GACGC 3 cut(s) 22, 317, 423
Csp6I GTAC 4 cut(s) 101, 655, 802, 930
CviAII CATG 3 cut(s) 388, 493, 641
CviQI GTAC 4 cut(s) 101, 655, 802, 930
DdeI CTNAG 4 cut(s) 620, 843, 873, 987
DpnI GATC 3 cut(s) 315, 392, 734
DpnII GATC 3 cut(s) 313, 390, 732
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco105I TACGTA 1 cut(s) 933
Eco47I GGWCC 2 cut(s) 244, 339
Eco91I GGTNACC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 289
EcoO65I GGTNACC 1 cut(s) 7
EcoRI GAATTC 1 cut(s) 562
EcoT22I ATGCAT 2 cut(s) 601, 642
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 3 cut(s) 391, 496, 644
FaqI GGGAC 1 cut(s) 978
FatI CATG 3 cut(s) 387, 492, 640
FauI CCCGC 1 cut(s) 207
FbaI TGATCA 2 cut(s) 390, 732
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 6 cut(s) 19, 272, 350, 508, 521, 605
FokI GGATG 3 cut(s) 90, 117, 1082
Fsp4HI GCNGC 6 cut(s) 19, 272, 350, 508, 521, 605
FspBI CTAG 3 cut(s) 437, 921, 1100
GlaI GCGC 2 cut(s) 662, 664
GluI GCNGC 6 cut(s) 19, 272, 350, 508, 521, 605
GsaI CCCAGC 1 cut(s) 349
HaeIII GGCC 3 cut(s) 271, 290, 321
HapII CCGG 7 cut(s) 5, 30, 260, 456, 710, 1066, 1111
HgaI GACGC 3 cut(s) 22, 317, 423
HhaI GCGC 2 cut(s) 663, 665
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 3 cut(s) 391, 496, 644
Hin6I GCGC 2 cut(s) 661, 663
HinP1I GCGC 2 cut(s) 661, 663
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HinfI GANTC 4 cut(s) 396, 496, 583, 706
HpaI GTTAAC 1 cut(s) 1035
HpaII CCGG 7 cut(s) 5, 30, 260, 456, 710, 1066, 1111
HphI GGTGA 6 cut(s) 19, 19, 28, 56, 162, 376
Hpy166II GTNNAC 5 cut(s) 64, 283, 655, 825, 1035
Hpy188I TCNGA 6 cut(s) 50, 206, 307, 335, 395, 568
Hpy188III TCNNGA 7 cut(s) 235, 247, 587, 710, 788, 1025, 1111
Hpy8I GTNNAC 5 cut(s) 64, 283, 655, 825, 1035
Hpy99I CGWCG 1 cut(s) 410
HpyAV CCTTC 6 cut(s) 32, 77, 685, 853, 1049, 1111
HpyCH4IV ACGT 5 cut(s) 99, 239, 716, 932, 1046
HpyCH4V TGCA 9 cut(s) 576, 599, 640, 760, 773, 778, 951, 983, 1168
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 667
HpyF3I CTNAG 4 cut(s) 620, 843, 873, 987
HpySE526I ACGT 5 cut(s) 99, 239, 716, 932, 1046
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 3 cut(s) 391, 496, 644
HspAI GCGC 2 cut(s) 661, 663
Kpn2I TCCGGA 2 cut(s) 709, 1110
Ksp22I TGATCA 2 cut(s) 390, 732
KspAI GTTAAC 1 cut(s) 1035
Kzo9I GATC 3 cut(s) 313, 390, 732
LmnI GCTCC 2 cut(s) 207, 458
Lsp1109I GCAGC 4 cut(s) 361, 494, 507, 591
LweI GCATC 2 cut(s) 760, 970
MaeI CTAG 3 cut(s) 437, 921, 1100
MaeII ACGT 5 cut(s) 99, 239, 716, 932, 1046
MaeIII GTNAC 3 cut(s) 7, 34, 135
MalI GATC 3 cut(s) 315, 392, 734
MbiI CCGCTC 2 cut(s) 202, 274
MboI GATC 3 cut(s) 313, 390, 732
MboII GAAGA 3 cut(s) 62, 455, 525
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 5 cut(s) 230, 468, 487, 562, 571
MmeI TCCRAC 1 cut(s) 1032
Mph1103I ATGCAT 2 cut(s) 601, 642
MroI TCCGGA 2 cut(s) 709, 1110
MseI TTAA 3 cut(s) 630, 1034, 1157
MspA1I CMGCKG 1 cut(s) 352
MspI CCGG 7 cut(s) 5, 30, 260, 456, 710, 1066, 1111
MspR9I CCNGG 2 cut(s) 261, 1067
MvnI CGCG 3 cut(s) 311, 663, 789
MwoI GCNNNNNNNGC 2 cut(s) 296, 667
NciI CCSGG 2 cut(s) 261, 1067
NdeII GATC 3 cut(s) 313, 390, 732
NlaIII CATG 3 cut(s) 391, 496, 644
NlaIV GGNNCC 3 cut(s) 245, 460, 918
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 3 cut(s) 7, 34, 135
NruI TCGCGA 1 cut(s) 789
NsiI ATGCAT 2 cut(s) 601, 642
NspI RCATGY 1 cut(s) 644
NspV TTCGAA 1 cut(s) 749
PauI GCGCGC 1 cut(s) 661
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 282
PfeI GAWTC 4 cut(s) 396, 496, 583, 706
PkrI GCNGC 6 cut(s) 20, 273, 351, 509, 522, 606
Ppu21I YACGTR 2 cut(s) 100, 933
PshAI GACNNNNGTC 1 cut(s) 242
PsiI TTATAA 1 cut(s) 549
Psp1406I AACGTT 1 cut(s) 1046
PspEI GGTNACC 1 cut(s) 7
PspFI CCCAGC 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 245, 460, 918
PspPI GGNCC 4 cut(s) 244, 269, 289, 339
PsuI RGATCY 1 cut(s) 313
PteI GCGCGC 1 cut(s) 661
RruI TCGCGA 1 cut(s) 789
RsaI GTAC 4 cut(s) 102, 656, 803, 931
RsaNI GTAC 4 cut(s) 101, 655, 802, 930
SaqAI TTAA 3 cut(s) 630, 1034, 1157
SatI GCNGC 6 cut(s) 19, 272, 350, 508, 521, 605
Sau3AI GATC 3 cut(s) 313, 390, 732
Sau96I GGNCC 4 cut(s) 244, 269, 289, 339
ScrFI CCNGG 2 cut(s) 261, 1067
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 2 cut(s) 760, 970
SfuI TTCGAA 1 cut(s) 749
SgrAI CRCCGGYG 1 cut(s) 29
SinI GGWCC 2 cut(s) 244, 339
SnaBI TACGTA 1 cut(s) 933
Sse9I AATT 5 cut(s) 230, 468, 487, 562, 571
SspMI CTAG 3 cut(s) 437, 921, 1100
StyD4I CCNGG 2 cut(s) 259, 1065
TaiI ACGT 5 cut(s) 102, 242, 719, 935, 1049
TaqI TCGA 9 cut(s) 234, 276, 324, 405, 441, 499, 680, 749, 1083
TasI AATT 5 cut(s) 230, 468, 487, 562, 571
TatI WGTACW 1 cut(s) 654
TauI GCSGC 2 cut(s) 21, 274
TfiI GAWTC 4 cut(s) 396, 496, 583, 706
Tru1I TTAA 3 cut(s) 630, 1034, 1157
Tru9I TTAA 3 cut(s) 630, 1034, 1157
TseFI GTSAC 3 cut(s) 7, 34, 135
TseI GCWGC 4 cut(s) 349, 507, 520, 604
Tsp45I GTSAC 3 cut(s) 7, 34, 135
TspDTI ATGAA 3 cut(s) 704, 1145, 1149
TspGWI ACGGA 3 cut(s) 111, 123, 982
VpaK11BI GGWCC 2 cut(s) 244, 339
XapI RAATTY 1 cut(s) 562
XceI RCATGY 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 824
XspI CTAG 3 cut(s) 437, 921, 1100
Zsp2I ATGCAT 2 cut(s) 601, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.