FvH4_3g25580

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013547
Physical Location & Seq
Reverse (-)
18489766 .. 18494172
4407 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25580.t1

Sequence Viewer

Length: 1122 bp
ATGCCGGCGATGTCGTTTCGTCGCCTTCTCACCTTTACCCTCAGACAATGCCCGCCTCTCTCCTCCCGCCACGTCATCCTCCCGTCTCTCTATCACCGCCTCCTCTCCTCCTCCGCCCGAAACCCTCCGGACTCATTCCGGACCCGAACCCCGCTGGAGAAGCAATTCGAAACATGGATCCACAAACTCAAACCCGGCTTCGGCCCACCCGACGTCGACGAGGCCCTCAAGGCCCAATCGGACCCCGATCTCGCCCTCGACATTTTCCGGTGGACCGCCCAGCAGCGCAACTACAAGCACAGCCACTCCACCTACCTCACCATGATCAAGATCCTCATCGACGGCCGCCGCTACCGCCACGCCGAAACGCTTCTCGAAGAGGTAGTCGCCGGAGCTTGTGAGATTAGTGTCCCTCTTTATAACTCCATTATTCGATTCTGTTGTGGTAGGAAGATGCTTTTCAATCGGGCTTTTGATGTGTACAAGAAAATGTTGAATTCCGAGAGTTGTAAGCCCACATTGGAGACATATGCGTTGCTGCTTAATTCTTTGCTTAGGAGGTTTAATAACAAGACTGTTTGTTATGTGTATTTGCGGTCGGTGAGATCGTTGTCCAAGCAAATGAAGGCCTCCGGTGTGATTCCGGATACTTTCGTGTTGAATATGATCATTAAGGCTTATTCCAAGTGTTTGGAAGTTGATGAGGCGCTGCAGCTGTTTCGGGAAATGGGGTTGTATGGATGCGAGCCCAATGCTTATACTTATGGCTACATTGCTAAGGGGTTGTGTGAGAAGGGGAGGGTAGGTCAGGGTTTAGGGTTTTACAAGGAAATGAGGGGAAAGGGGTTGGTGCCCAGCAGTAGTACTTTTATGGTTGTGATTTGCAGTCTTGCTTTGGAGCGGAGATATGAGGATGCGAGTGATGTTGTGTTTGATATGTTGAGTAATTCTGTATCTCCTGATTTGTTGACTTATAAAACGTTATTGGAAGGGTTGTGCCGGGATGGAAAAGGTGAGGAGGCATTTGATTTGCTGGAGAATTTCAGGAGCAGGGATAGAGCCATGAATGAAAGGACATACAAGACATTGTTAAATGCTTTGCATTTTATAAATAAGGAGTAA

Protein Analysis

374

Amino Acids

42.83

Weight (kDa)

9.18

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 137 - 185 3e-06 PPR repeat family
PPR_long PF17177 148 - 285 1.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 212 - 243 9.5e-06 PPR repeat
PPR_2 PF13041 215 - 263 8.7e-14 PPR repeat family
PPR_long PF17177 271 - 366 7.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 285 - 334 6.3e-08 PPR repeat family
PPR_1 PF12854 318 - 346 5.4e-08 PPR repeat
PPR_2 PF13041 320 - 367 3.1e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25210
fragaria_vesca FvH4_3g25580
malus_domestica MD05G1166500.v1.1 MD10G1156500.v1.1
prunus_persica Prupe.8G194300_v2.0.a1
pyrus_communis pycom05g15510 pycom10g13470
rosa_chinensis RchiOBHm_Chr5g0046411
rosa_laevigata RLG00000034408
rosa_multiflora Rmu_sc0003878.1_g000050
rosa_roxburghii Rroxscaffold_1G00034990 Rroxscaffold_7G00197530
rosa_rugosa Rorug05G0227800
rosa_samantha Rh5AG309000 Rh5BG317200 Rh5CG343100 Rh5DG327400
rosa_wichuraiana Rw0G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 420, 975, 1109
AatII GACGTC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 850
AccBSI CCGCTC 1 cut(s) 901
AccI GTMKAC 1 cut(s) 216
AccIII TCCGGA 3 cut(s) 127, 138, 643
AclI AACGTT 1 cut(s) 980
AclWI GGATC 3 cut(s) 172, 185, 325
AcoI YGGCCR 1 cut(s) 343
AcsI RAATTY 2 cut(s) 496, 1039
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 482, 865
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 3 cut(s) 463, 496, 661
AjiI CACGTC 1 cut(s) 73
AluBI AGCT 2 cut(s) 395, 715
AluI AGCT 2 cut(s) 395, 715
Alw26I GTCTC 2 cut(s) 90, 518
AlwI GGATC 3 cut(s) 172, 185, 325
Aor13HI TCCGGA 3 cut(s) 127, 138, 643
AoxI GGCC 5 cut(s) 202, 222, 231, 343, 627
ApeKI GCWGC 4 cut(s) 283, 538, 709, 712
ApoI RAATTY 2 cut(s) 496, 1039
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
Asp700I GAANNNNTTC 2 cut(s) 164, 369
AspLEI GCGC 2 cut(s) 288, 709
AspS9I GGNCC 6 cut(s) 141, 203, 223, 232, 241, 273
AsuC2I CCSGG 2 cut(s) 195, 1001
AsuHPI GGTGA 5 cut(s) 22, 86, 310, 613, 1025
AsuII TTCGAA 1 cut(s) 168
AvaII GGWCC 3 cut(s) 141, 241, 273
BaeGI GKGCMC 1 cut(s) 855
BaeI ACNNNNGTAYC 2 cut(s) 639, 672
BamHI GGATCC 1 cut(s) 177
BanI GGYRCC 1 cut(s) 850
BanII GRGCYC 1 cut(s) 750
BbvI GCAGC 4 cut(s) 295, 525, 696, 724
BccI CCATC 1 cut(s) 998
BceAI ACGGC 1 cut(s) 358
BcgI CGANNNNNNTGC 4 cut(s) 701, 734, 735, 768
BciVI GTATCC 1 cut(s) 640
BclI TGATCA 2 cut(s) 324, 666
BcnI CCSGG 2 cut(s) 195, 1001
BcoDI GTCTC 2 cut(s) 90, 518
BfmI CTRYAG 1 cut(s) 710
BfoI RGCGCY 1 cut(s) 710
BfuI GTATCC 1 cut(s) 640
BglI GCCNNNNNGGC 1 cut(s) 230
BisI GCNGC 6 cut(s) 284, 346, 349, 539, 710, 713
BlsI GCNGC 6 cut(s) 285, 347, 350, 540, 711, 714
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 2 cut(s) 195, 1001
Bme18I GGWCC 3 cut(s) 141, 241, 273
BmgBI CACGTC 1 cut(s) 73
BmgT120I GGNCC 6 cut(s) 141, 203, 223, 232, 241, 273
BmiI GGNNCC 4 cut(s) 143, 179, 243, 852
BmrFI CCNGG 2 cut(s) 195, 1001
BmsI GCATC 3 cut(s) 444, 731, 904
BpmI CTGGAG 2 cut(s) 176, 1055
Bpu10I CCTNAGC 2 cut(s) 554, 777
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 2 cut(s) 195, 1001
BsaBI GATNNNNATC 2 cut(s) 329, 335
BsaHI GRCGYC 1 cut(s) 213
BsaWI WCCGGW 5 cut(s) 127, 138, 267, 632, 643
BsaXI ACNNNNNCTCC 4 cut(s) 290, 320, 790, 820
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 4
Bse3DI GCAATG 1 cut(s) 771
Bse8I GATNNNNATC 2 cut(s) 329, 335
BseAI TCCGGA 3 cut(s) 127, 138, 643
BseGI GGATG 4 cut(s) 75, 746, 919, 1009
BseJI GATNNNNATC 2 cut(s) 329, 335
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 1 cut(s) 55
BseRI GAGGAG 5 cut(s) 52, 92, 97, 100, 1031
BseSI GKGCMC 1 cut(s) 855
BseX3I CGGCCG 1 cut(s) 343
BseXI GCAGC 4 cut(s) 295, 525, 696, 724
BseYI CCCAGC 2 cut(s) 279, 854
Bsh1285I CGRYCG 2 cut(s) 346, 599
BshFI GGCC 5 cut(s) 204, 224, 233, 345, 629
BshNI GGYRCC 1 cut(s) 850
BsiEI CGRYCG 2 cut(s) 346, 599
BsiSI CCGG 9 cut(s) 5, 128, 139, 195, 268, 390, 633, 644, 1000
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 2 cut(s) 90, 518
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 5 cut(s) 204, 224, 233, 345, 629
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 2 cut(s) 750, 855
Bsp13I TCCGGA 3 cut(s) 127, 138, 643
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 6 cut(s) 177, 247, 324, 330, 605, 666
BspANI GGCC 5 cut(s) 204, 224, 233, 345, 629
BspCNI CTCAG 1 cut(s) 54
BspEI TCCGGA 3 cut(s) 127, 138, 643
BspLI GGNNCC 4 cut(s) 143, 179, 243, 852
BspMAI CTGCAG 1 cut(s) 714
BspPI GGATC 3 cut(s) 172, 185, 325
BspT104I TTCGAA 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 850
BsrBI CCGCTC 1 cut(s) 901
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 480
BssAI RCCGGY 1 cut(s) 4
BssMI GATC 6 cut(s) 177, 247, 324, 330, 605, 666
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 213
BstAUI TGTACA 1 cut(s) 480
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 6, 53, 746
BstDEI CTNAG 3 cut(s) 41, 554, 777
BstF5I GGATG 4 cut(s) 75, 746, 919, 1009
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 2 cut(s) 288, 709
BstKTI GATC 6 cut(s) 180, 250, 327, 333, 608, 669
BstMAI GTCTC 2 cut(s) 90, 518
BstMBI GATC 6 cut(s) 177, 247, 324, 330, 605, 666
BstMCI CGRYCG 2 cut(s) 346, 599
BstMWI GCNNNNNNNGC 3 cut(s) 160, 230, 354
BstSCI CCNGG 2 cut(s) 193, 999
BstSFI CTRYAG 1 cut(s) 710
BstSLI GKGCMC 1 cut(s) 855
BstV1I GCAGC 4 cut(s) 295, 525, 696, 724
BstX2I RGATCY 2 cut(s) 177, 330
BstXI CCANNNNNNTGG 1 cut(s) 691
BstYI RGATCY 2 cut(s) 177, 330
BstZI CGGCCG 1 cut(s) 343
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 5 cut(s) 204, 224, 233, 345, 629
BtgZI GCGATG 1 cut(s) 23
BtrI CACGTC 1 cut(s) 73
BtsCI GGATG 4 cut(s) 75, 746, 919, 1009
Cac8I GCNNGC 3 cut(s) 6, 53, 746
CfoI GCGC 2 cut(s) 288, 709
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 6 cut(s) 141, 203, 223, 232, 241, 273
Csp6I GTAC 2 cut(s) 481, 864
CviAII CATG 3 cut(s) 174, 322, 1063
CviQI GTAC 2 cut(s) 481, 864
DdeI CTNAG 3 cut(s) 41, 554, 777
DpnI GATC 6 cut(s) 179, 249, 326, 332, 607, 668
DpnII GATC 6 cut(s) 177, 247, 324, 330, 605, 666
EaeI YGGCCR 1 cut(s) 343
EagI CGGCCG 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
EciI GGCGGA 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 343
Eco147I AGGCCT 1 cut(s) 629
Eco24I GRGCYC 1 cut(s) 750
Eco47I GGWCC 3 cut(s) 141, 241, 273
Eco52I CGGCCG 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 750
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 3 cut(s) 177, 325, 1066
FaqI GGGAC 1 cut(s) 395
FatI CATG 3 cut(s) 173, 321, 1062
FauI CCCGC 3 cut(s) 60, 74, 159
FauNDI CATATG 1 cut(s) 529
FbaI TGATCA 2 cut(s) 324, 666
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 6 cut(s) 284, 346, 349, 539, 710, 713
FokI GGATG 4 cut(s) 62, 753, 926, 1016
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 6 cut(s) 284, 346, 349, 539, 710, 713
GlaI GCGC 2 cut(s) 287, 708
GluI GCNGC 6 cut(s) 284, 346, 349, 539, 710, 713
GsaI CCCAGC 2 cut(s) 283, 858
GsuI CTGGAG 2 cut(s) 176, 1055
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 5 cut(s) 204, 224, 233, 345, 629
HapII CCGG 9 cut(s) 5, 128, 139, 195, 268, 390, 633, 644, 1000
HhaI GCGC 2 cut(s) 288, 709
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 3 cut(s) 177, 325, 1066
Hin6I GCGC 2 cut(s) 286, 707
HinP1I GCGC 2 cut(s) 286, 707
HincII GTYRAC 2 cut(s) 217, 969
HindII GTYRAC 2 cut(s) 217, 969
HinfI GANTC 3 cut(s) 131, 435, 640
HpaII CCGG 9 cut(s) 5, 128, 139, 195, 268, 390, 633, 644, 1000
HphI GGTGA 5 cut(s) 22, 86, 310, 613, 1025
Hpy166II GTNNAC 4 cut(s) 217, 273, 481, 969
Hpy188I TCNGA 3 cut(s) 44, 241, 502
Hpy188III TCNNGA 8 cut(s) 128, 139, 328, 374, 644, 722, 959, 1045
Hpy8I GTNNAC 4 cut(s) 217, 273, 481, 969
Hpy99I CGWCG 5 cut(s) 24, 215, 218, 221, 344
HpyAV CCTTC 4 cut(s) 35, 619, 787, 983
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 3 cut(s) 72, 213, 980
HpyCH4V TGCA 3 cut(s) 712, 885, 1102
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 230, 354
HpyF3I CTNAG 3 cut(s) 41, 554, 777
HpySE526I ACGT 3 cut(s) 72, 213, 980
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 3 cut(s) 177, 325, 1066
HspAI GCGC 2 cut(s) 286, 707
Kpn2I TCCGGA 3 cut(s) 127, 138, 643
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 324, 666
Kzo9I GATC 6 cut(s) 177, 247, 324, 330, 605, 666
LmnI GCTCC 3 cut(s) 392, 898, 1047
Lsp1109I GCAGC 4 cut(s) 295, 525, 696, 724
LweI GCATC 3 cut(s) 444, 731, 904
MaeII ACGT 3 cut(s) 72, 213, 980
MalI GATC 6 cut(s) 179, 249, 326, 332, 607, 668
MbiI CCGCTC 1 cut(s) 901
MboI GATC 6 cut(s) 177, 247, 324, 330, 605, 666
MboII GAAGA 2 cut(s) 389, 463
MflI RGATCY 2 cut(s) 177, 330
MhlI GDGCHC 2 cut(s) 750, 855
MluCI AATT 5 cut(s) 164, 496, 544, 946, 1039
MlyI GAGTC 1 cut(s) 125
MroI TCCGGA 3 cut(s) 127, 138, 643
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 164, 369
MseI TTAA 4 cut(s) 543, 564, 672, 1091
MspA1I CMGCKG 2 cut(s) 154, 715
MspI CCGG 9 cut(s) 5, 128, 139, 195, 268, 390, 633, 644, 1000
MspR9I CCNGG 2 cut(s) 195, 1001
MwoI GCNNNNNNNGC 3 cut(s) 160, 230, 354
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 195, 1001
NdeI CATATG 1 cut(s) 529
NdeII GATC 6 cut(s) 177, 247, 324, 330, 605, 666
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 3 cut(s) 177, 325, 1066
NlaIV GGNNCC 4 cut(s) 143, 179, 243, 852
NspV TTCGAA 1 cut(s) 168
PceI AGGCCT 1 cut(s) 629
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 605
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 164, 369
PfeI GAWTC 2 cut(s) 435, 640
PkrI GCNGC 6 cut(s) 285, 347, 350, 540, 711, 714
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PsiI TTATAA 3 cut(s) 420, 975, 1109
Psp1406I AACGTT 1 cut(s) 980
PspFI CCCAGC 2 cut(s) 279, 854
PspN4I GGNNCC 4 cut(s) 143, 179, 243, 852
PspPI GGNCC 6 cut(s) 141, 203, 223, 232, 241, 273
PstI CTGCAG 1 cut(s) 714
PsuI RGATCY 2 cut(s) 177, 330
PvuII CAGCTG 1 cut(s) 715
RsaI GTAC 2 cut(s) 482, 865
RsaNI GTAC 2 cut(s) 481, 864
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 4 cut(s) 543, 564, 672, 1091
SatI GCNGC 6 cut(s) 284, 346, 349, 539, 710, 713
Sau3AI GATC 6 cut(s) 177, 247, 324, 330, 605, 666
Sau96I GGNCC 6 cut(s) 141, 203, 223, 232, 241, 273
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 195, 1001
SduI GDGCHC 2 cut(s) 750, 855
SfaNI GCATC 3 cut(s) 444, 731, 904
SfcI CTRYAG 1 cut(s) 710
SfiI GGCCNNNNNGGCC 1 cut(s) 230
SfuI TTCGAA 1 cut(s) 168
SgrDI CGTCGACG 1 cut(s) 215
SinI GGWCC 3 cut(s) 141, 241, 273
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 5 cut(s) 164, 496, 544, 946, 1039
SseBI AGGCCT 1 cut(s) 629
StuI AGGCCT 1 cut(s) 629
StyD4I CCNGG 2 cut(s) 193, 999
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 3 cut(s) 75, 216, 983
TaqI TCGA 6 cut(s) 168, 216, 258, 339, 375, 433
TasI AATT 5 cut(s) 164, 496, 544, 946, 1039
TatI WGTACW 2 cut(s) 480, 863
TauI GCSGC 2 cut(s) 348, 351
TfiI GAWTC 2 cut(s) 435, 640
Tru1I TTAA 4 cut(s) 543, 564, 672, 1091
Tru9I TTAA 4 cut(s) 543, 564, 672, 1091
TseI GCWGC 4 cut(s) 283, 538, 709, 712
TspDTI ATGAA 3 cut(s) 638, 1079, 1083
VpaK11BI GGWCC 3 cut(s) 141, 241, 273
XapI RAATTY 2 cut(s) 496, 1039
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 164, 369
ZraI GACGTC 1 cut(s) 214
ZrmI AGTACT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.