Rw0G021510

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00991
OG0013547
Physical Location & Seq
Forward (+)
9678 .. 11681
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G021510.1

Sequence Viewer

Length: 1173 bp
ATGCCGGCGACGTTACGTCGCGTTCTCACCGCTACTCTCAGACGCCCCAATATCCTCTTTCCTCAACGCCCACCTCTCTCCTCCCGCCACGTAATCCCTGACCCTTCATTTCCGGTCACCCTCCCGTCACTCTATCACCGCCTCCTATCCTCCTCCTCCGCCGCCGCCCAAAACCCTCCGGACTCATTCCGAACCCGAACCCCACTGGAGAAGCAATTCGAAACATGGGTCCAGAAACTCAAACCCGGATTCGGCCCACCCGACGTCGACGAGGCCCTGAAAGCCCAATCGGATCCTGACCTCGCTCTCGACATTTTCCGATGGACGGCCCAGCAGCGCAACTACAAGCACAACCACTCCACCTACCTCACCATGATCAAAATCCTGATCAACGGCCGCCGCTACCGCCACGCCGAAACCCTACTTGAGGAGGTGGTCGCCGGAGCTTGTGAGATAAGTGTCCCGCTCTATAACTCCATTATTCGATTCTGTTGTGGAAGAAAGATGCTCTTCAATCGCGCTTTTGATGTGTACAAGAAAATGTTGAATTCGGAGAACTGTAAGCCCACATTGGAGACCTATGCATTGCTGCTTAATTCATTGCTCAGGAGGTTTGATAATATCACTGTTTGCCATGTGTATTTGCGGTCGGTGAGGTCGTTGTCGAAGCAAATGAAGGCATCCGGTGTGATACCGGATACTTTTGTGTTGAATATGATCATCAAGGCTTATTCCAAGTGTTTGGAAGTTGATGAGGCACTTCAGGTTTTTCGGGAAATGGGGTTGTATGGATGTGAGCCGAATGGTTATACTTATGGTTACATTGCCAAAGGGTTGTGTGAGAAGGGGAGGGTAGGTCAGGGTTTAGGGTTTTACAGGGAAATGAGAGGGAAGGGTTTGGTGCCTAGCAGTAGTACTTACATGGTTGTAATTTGCAGTCTTGCTCTGGAGCGGAGATATGAGGATGCGAGTAATGTCGTGTTTGATATGTTGAGTAATTCTATGTCTCCTGATTTGTTAACTTACAAAACTTTGTTGGAAGGATTGTGCCGGGATGGGAAAGGTGAGGAGGCATTTGATCTACTAGAGACTTTTAGGAGCAGGGATAGAGCCATGAATGAAAAGACATACAAGACATTGTTAAATGCATTGCATTTTGTAAATAGGGAGTAG

Protein Analysis

390

Amino Acids

44.53

Weight (kDa)

9.23

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 154 - 202 1.4e-06 PPR repeat family
PPR_long PF17177 165 - 301 3e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 232 - 280 8.8e-13 PPR repeat family
PPR_long PF17177 288 - 383 3.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 302 - 351 6.6e-08 PPR repeat family
PPR_1 PF12854 335 - 363 7.1e-08 PPR repeat
PPR_2 PF13041 337 - 384 6e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25210
fragaria_vesca FvH4_3g25580
malus_domestica MD05G1166500.v1.1 MD10G1156500.v1.1
prunus_persica Prupe.8G194300_v2.0.a1
pyrus_communis pycom05g15510 pycom10g13470
rosa_chinensis RchiOBHm_Chr5g0046411
rosa_laevigata RLG00000034408
rosa_multiflora Rmu_sc0003878.1_g000050
rosa_roxburghii Rroxscaffold_1G00034990 Rroxscaffold_7G00197530
rosa_rugosa Rorug05G0227800
rosa_samantha Rh5AG309000 Rh5BG317200 Rh5CG343100 Rh5DG327400
rosa_wichuraiana Rw0G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 901
AccBSI CCGCTC 2 cut(s) 466, 952
AccI GTMKAC 1 cut(s) 267
AccII CGCG 2 cut(s) 21, 519
AccIII TCCGGA 1 cut(s) 178
AclWI GGATC 2 cut(s) 287, 300
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 746
AcyI GRCGYC 2 cut(s) 43, 264
AfaI GTAC 2 cut(s) 533, 916
AfiI CCNNNNNNNGG 3 cut(s) 251, 325, 427
AgsI TTSAA 3 cut(s) 514, 547, 712
AhdI GACNNNNNGTC 1 cut(s) 15
AluBI AGCT 1 cut(s) 446
AluI AGCT 1 cut(s) 446
Alw26I GTCTC 3 cut(s) 569, 1011, 1082
AlwI GGATC 2 cut(s) 287, 300
Aor13HI TCCGGA 1 cut(s) 178
AoxI GGCC 4 cut(s) 253, 273, 327, 394
ApeKI GCWGC 2 cut(s) 334, 589
ApoI RAATTY 1 cut(s) 547
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 339, 521
AspS9I GGNCC 4 cut(s) 229, 254, 274, 328
AsuC2I CCSGG 2 cut(s) 246, 1052
AsuHPI GGTGA 6 cut(s) 19, 109, 128, 361, 664, 1076
AsuII TTCGAA 1 cut(s) 219
AvaII GGWCC 1 cut(s) 229
BaeI ACNNNNGTAYC 2 cut(s) 690, 723
BamHI GGATCC 1 cut(s) 292
BanI GGYRCC 1 cut(s) 901
BbvI GCAGC 2 cut(s) 346, 576
BccI CCATC 2 cut(s) 315, 1049
BceAI ACGGC 2 cut(s) 342, 409
BciVI GTATCC 1 cut(s) 691
BclI TGATCA 3 cut(s) 375, 387, 717
BcnI CCSGG 2 cut(s) 246, 1052
BcoDI GTCTC 3 cut(s) 569, 1011, 1082
BfaI CTAG 2 cut(s) 906, 1085
BfuI GTATCC 1 cut(s) 691
BisI GCNGC 6 cut(s) 162, 165, 335, 397, 400, 590
BlsI GCNGC 6 cut(s) 163, 166, 336, 398, 401, 591
BmcAI AGTACT 1 cut(s) 916
Bme1390I CCNGG 2 cut(s) 246, 1052
Bme18I GGWCC 1 cut(s) 229
BmeRI GACNNNNNGTC 1 cut(s) 15
BmgT120I GGNCC 4 cut(s) 229, 254, 274, 328
BmiI GGNNCC 3 cut(s) 230, 294, 903
BmrFI CCNGG 2 cut(s) 246, 1052
BmsI GCATC 3 cut(s) 495, 689, 955
BpmI CTGGAG 2 cut(s) 227, 968
Bpu10I CCTNAGC 1 cut(s) 605
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 1 cut(s) 446
BpuMI CCSGG 2 cut(s) 246, 1052
BsaAI YACGTR 1 cut(s) 91
BsaBI GATNNNNATC 1 cut(s) 380
BsaHI GRCGYC 2 cut(s) 43, 264
BsaI GGTCTC 1 cut(s) 569
BsaWI WCCGGW 4 cut(s) 112, 178, 683, 694
BsaXI ACNNNNNCTCC 4 cut(s) 341, 371, 841, 871
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 325, 427
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 1 cut(s) 210
Bse3DI GCAATG 4 cut(s) 584, 599, 822, 1148
Bse8I GATNNNNATC 1 cut(s) 380
BseAI TCCGGA 1 cut(s) 178
BseGI GGATG 4 cut(s) 680, 797, 970, 1060
BseJI GATNNNNATC 1 cut(s) 380
BseLI CCNNNNNNNGG 3 cut(s) 251, 325, 427
BseMI GCAATG 4 cut(s) 584, 599, 822, 1148
BseMII CTCAG 2 cut(s) 52, 619
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 5 cut(s) 70, 142, 145, 443, 1082
BseX3I CGGCCG 1 cut(s) 394
BseXI GCAGC 2 cut(s) 346, 576
BseYI CCCAGC 1 cut(s) 330
Bsh1236I CGCG 2 cut(s) 21, 519
Bsh1285I CGRYCG 2 cut(s) 397, 650
BshFI GGCC 4 cut(s) 255, 275, 329, 396
BshNI GGYRCC 1 cut(s) 901
BsiEI CGRYCG 2 cut(s) 397, 650
BsiSI CCGG 8 cut(s) 5, 113, 179, 246, 441, 684, 695, 1051
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 3 cut(s) 251, 325, 427
BsmAI GTCTC 3 cut(s) 569, 1011, 1082
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 4 cut(s) 255, 275, 329, 396
Bso31I GGTCTC 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 219
Bsp13I TCCGGA 1 cut(s) 178
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 5 cut(s) 292, 375, 387, 717, 1078
BspANI GGCC 4 cut(s) 255, 275, 329, 396
BspCNI CTCAG 2 cut(s) 51, 618
BspEI TCCGGA 1 cut(s) 178
BspFNI CGCG 2 cut(s) 21, 519
BspLI GGNNCC 3 cut(s) 230, 294, 903
BspPI GGATC 2 cut(s) 287, 300
BspQI GCTCTTC 1 cut(s) 515
BspT104I TTCGAA 1 cut(s) 219
BspT107I GGYRCC 1 cut(s) 901
BspTNI GGTCTC 1 cut(s) 569
BsrBI CCGCTC 2 cut(s) 466, 952
BsrDI GCAATG 4 cut(s) 584, 599, 822, 1148
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 4
BssMI GATC 5 cut(s) 292, 375, 387, 717, 1078
BssNI GRCGYC 2 cut(s) 43, 264
Bst4CI ACNGT 2 cut(s) 560, 628
Bst6I CTCTTC 1 cut(s) 515
BstACI GRCGYC 2 cut(s) 43, 264
BstAUI TGTACA 1 cut(s) 531
BstBAI YACGTR 1 cut(s) 91
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 38, 605
BstEII GGTNACC 1 cut(s) 115
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 4 cut(s) 680, 797, 970, 1060
BstFNI CGCG 2 cut(s) 21, 519
BstHHI GCGC 2 cut(s) 339, 521
BstKTI GATC 5 cut(s) 295, 378, 390, 720, 1081
BstMAI GTCTC 3 cut(s) 569, 1011, 1082
BstMBI GATC 5 cut(s) 292, 375, 387, 717, 1078
BstMCI CGRYCG 2 cut(s) 397, 650
BstMWI GCNNNNNNNGC 2 cut(s) 281, 405
BstPI GGTNACC 1 cut(s) 115
BstSCI CCNGG 2 cut(s) 244, 1050
BstUI CGCG 2 cut(s) 21, 519
BstV1I GCAGC 2 cut(s) 346, 576
BstX2I RGATCY 1 cut(s) 292
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 1 cut(s) 292
BstZI CGGCCG 1 cut(s) 394
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 4 cut(s) 255, 275, 329, 396
BtsCI GGATG 4 cut(s) 680, 797, 970, 1060
BtsIMutI CAGTG 2 cut(s) 203, 624
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 339, 521
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 4 cut(s) 229, 254, 274, 328
CseI GACGC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 532, 915
CviAII CATG 5 cut(s) 225, 373, 635, 922, 1114
CviQI GTAC 2 cut(s) 532, 915
DdeI CTNAG 2 cut(s) 38, 605
DpnI GATC 5 cut(s) 294, 377, 389, 719, 1080
DpnII GATC 5 cut(s) 292, 375, 387, 717, 1078
DriI GACNNNNNGTC 1 cut(s) 15
EaeI YGGCCR 1 cut(s) 394
EagI CGGCCG 1 cut(s) 394
Eam1104I CTCTTC 1 cut(s) 515
Eam1105I GACNNNNNGTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 148
EclXI CGGCCG 1 cut(s) 394
Eco31I GGTCTC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 229
Eco52I CGGCCG 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 746
Eco91I GGTNACC 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 274
EcoO65I GGTNACC 1 cut(s) 115
EcoRI GAATTC 1 cut(s) 547
EcoT22I ATGCAT 2 cut(s) 586, 1150
FaeI CATG 5 cut(s) 228, 376, 638, 925, 1117
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 1 cut(s) 446
FatI CATG 5 cut(s) 224, 372, 634, 921, 1113
FauI CCCGC 2 cut(s) 92, 471
FbaI TGATCA 3 cut(s) 375, 387, 717
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 6 cut(s) 162, 165, 335, 397, 400, 590
FokI GGATG 4 cut(s) 667, 804, 977, 1067
Fsp4HI GCNGC 6 cut(s) 162, 165, 335, 397, 400, 590
FspBI CTAG 2 cut(s) 906, 1085
GlaI GCGC 2 cut(s) 338, 520
GluI GCNGC 6 cut(s) 162, 165, 335, 397, 400, 590
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 2 cut(s) 227, 968
HaeIII GGCC 4 cut(s) 255, 275, 329, 396
HapII CCGG 8 cut(s) 5, 113, 179, 246, 441, 684, 695, 1051
HgaI GACGC 1 cut(s) 51
HhaI GCGC 2 cut(s) 339, 521
Hin1I GRCGYC 2 cut(s) 43, 264
Hin1II CATG 5 cut(s) 228, 376, 638, 925, 1117
Hin6I GCGC 2 cut(s) 337, 519
HinP1I GCGC 2 cut(s) 337, 519
HincII GTYRAC 2 cut(s) 268, 1020
HindII GTYRAC 2 cut(s) 268, 1020
HinfI GANTC 3 cut(s) 182, 249, 486
HpaI GTTAAC 1 cut(s) 1020
HpaII CCGG 8 cut(s) 5, 113, 179, 246, 441, 684, 695, 1051
HphI GGTGA 6 cut(s) 19, 109, 128, 361, 664, 1076
Hpy166II GTNNAC 3 cut(s) 268, 532, 1020
Hpy188I TCNGA 5 cut(s) 41, 191, 292, 320, 553
Hpy188III TCNNGA 9 cut(s) 179, 232, 296, 308, 385, 607, 773, 947, 1010
Hpy8I GTNNAC 3 cut(s) 268, 532, 1020
Hpy99I CGWCG 5 cut(s) 13, 21, 266, 269, 272
HpyAV CCTTC 5 cut(s) 114, 670, 838, 886, 1034
HpyCH4III ACNGT 2 cut(s) 560, 628
HpyCH4IV ACGT 4 cut(s) 11, 16, 90, 264
HpyCH4V TGCA 4 cut(s) 584, 936, 1148, 1153
HpyF10VI GCNNNNNNNGC 2 cut(s) 281, 405
HpyF3I CTNAG 2 cut(s) 38, 605
HpySE526I ACGT 4 cut(s) 11, 16, 90, 264
Hsp92I GRCGYC 2 cut(s) 43, 264
Hsp92II CATG 5 cut(s) 228, 376, 638, 925, 1117
HspAI GCGC 2 cut(s) 337, 519
Kpn2I TCCGGA 1 cut(s) 178
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 3 cut(s) 375, 387, 717
KspAI GTTAAC 1 cut(s) 1020
Kzo9I GATC 5 cut(s) 292, 375, 387, 717, 1078
LguI GCTCTTC 1 cut(s) 515
LmnI GCTCC 3 cut(s) 443, 949, 1098
Lsp1109I GCAGC 2 cut(s) 346, 576
LweI GCATC 3 cut(s) 495, 689, 955
MaeI CTAG 2 cut(s) 906, 1085
MaeII ACGT 4 cut(s) 11, 16, 90, 264
MaeIII GTNAC 4 cut(s) 12, 115, 126, 818
MalI GATC 5 cut(s) 294, 377, 389, 719, 1080
MbiI CCGCTC 2 cut(s) 466, 952
MboI GATC 5 cut(s) 292, 375, 387, 717, 1078
MboII GAAGA 2 cut(s) 502, 510
MflI RGATCY 1 cut(s) 292
MluCI AATT 5 cut(s) 215, 547, 595, 930, 997
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 1017
Mph1103I ATGCAT 2 cut(s) 586, 1150
MroI TCCGGA 1 cut(s) 178
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 3 cut(s) 594, 1019, 1142
MspI CCGG 8 cut(s) 5, 113, 179, 246, 441, 684, 695, 1051
MspR9I CCNGG 2 cut(s) 246, 1052
MvnI CGCG 2 cut(s) 21, 519
MwoI GCNNNNNNNGC 2 cut(s) 281, 405
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 2 cut(s) 246, 1052
NdeII GATC 5 cut(s) 292, 375, 387, 717, 1078
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 228, 376, 638, 925, 1117
NlaIV GGNNCC 3 cut(s) 230, 294, 903
NmuCI GTSAC 2 cut(s) 115, 126
NsiI ATGCAT 2 cut(s) 586, 1150
NspV TTCGAA 1 cut(s) 219
PciSI GCTCTTC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 656
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 2 cut(s) 249, 486
PkrI GCNGC 6 cut(s) 163, 166, 336, 398, 401, 591
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
Ppu21I YACGTR 1 cut(s) 91
PspEI GGTNACC 1 cut(s) 115
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 230, 294, 903
PspPI GGNCC 4 cut(s) 229, 254, 274, 328
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 2 cut(s) 533, 916
RsaNI GTAC 2 cut(s) 532, 915
SalI GTCGAC 1 cut(s) 266
SapI GCTCTTC 1 cut(s) 515
SaqAI TTAA 3 cut(s) 594, 1019, 1142
SatI GCNGC 6 cut(s) 162, 165, 335, 397, 400, 590
Sau3AI GATC 5 cut(s) 292, 375, 387, 717, 1078
Sau96I GGNCC 4 cut(s) 229, 254, 274, 328
ScaI AGTACT 1 cut(s) 916
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 2 cut(s) 246, 1052
SfaNI GCATC 3 cut(s) 495, 689, 955
SfuI TTCGAA 1 cut(s) 219
SgrDI CGTCGACG 1 cut(s) 266
SinI GGWCC 1 cut(s) 229
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 5 cut(s) 215, 547, 595, 930, 997
SspMI CTAG 2 cut(s) 906, 1085
StyD4I CCNGG 2 cut(s) 244, 1050
TaaI ACNGT 2 cut(s) 560, 628
TaiI ACGT 4 cut(s) 14, 19, 93, 267
TaqI TCGA 5 cut(s) 219, 267, 309, 484, 665
TasI AATT 5 cut(s) 215, 547, 595, 930, 997
TatI WGTACW 2 cut(s) 531, 914
TauI GCSGC 4 cut(s) 164, 167, 399, 402
TfiI GAWTC 2 cut(s) 249, 486
Tru1I TTAA 3 cut(s) 594, 1019, 1142
Tru9I TTAA 3 cut(s) 594, 1019, 1142
TscAI CASTG 2 cut(s) 210, 631
TseFI GTSAC 2 cut(s) 115, 126
TseI GCWGC 2 cut(s) 334, 589
Tsp45I GTSAC 2 cut(s) 115, 126
TspDTI ATGAA 5 cut(s) 96, 588, 689, 1130, 1134
TspRI CASTG 2 cut(s) 210, 631
VpaK11BI GGWCC 1 cut(s) 229
XagI CCTNNNNNAGG 1 cut(s) 425
XapI RAATTY 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 2 cut(s) 906, 1085
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 916
Zsp2I ATGCAT 2 cut(s) 586, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.