MD05G1166500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013547
Physical Location & Seq
Reverse (-)
29636387 .. 29637103
717 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1166500.v1.1.491

Sequence Viewer

Length: 717 bp
ATGCCCCTTTACAATTCCATGATTAGATTTTACTGCGGGAGAAAGCTGCTTTTCAATCGTGCTTTTGATGTTTATAAGAAAATGTTGAATTACGAGAATTGCAAACCGAATCTGGAAACTTATGCATTGCTGCTCAACTCTTTGCTTAGGAGGTTTAATAATATGCATGTTTGCTATGTGTACTTGCGAGCCGTGCGGTCTTTGGCGAAGCAGATGAAGGCGTGCGGTGTGATACCGGATACGTTTATGTTGAAAATGATCATAAAGGCGTATTCCAAGTGTTTGCAGGTTGATGATGCAATGCAGGTATTTCGCGAAATGGGGTTGTACGGATGTGAGCCGAACACCTATACTTATGGTTACATTGCTAAAGGGTTGTGTGAGAAGGGGAGGGTAGCTCAGGGTTTAGGGTTTTACAAGGAAATGAGAGGAAAAGGGTTGGTGCCTAGCAGTAGTACGTGTATGAATGTGATTTGCAGTCTCACCATGGGACGGAAGTATGATGATGCTATTGAGGTTGTGCTTGATATGTTGAGTAATGCTATGTCTCCTGATTTGTTAACTTACAAGACGTTATTGGAAGGATTGTGCCGGGATGGGAAAGGTGTCGAGGCATTTGATCTACTAGAGGATTTGCGGAGGAAGGATGCTGGTATGATGAATGAAAGGACTTACAAGACTTTGTTAAATGCTTTGCATTTTGTAAATGAGGAGTAG

Protein Analysis

239

Amino Acids

27.35

Weight (kDa)

8.91

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 4 - 49 1.3e-07 PPR repeat family
PPR_long PF17177 12 - 149 1.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 79 - 127 7e-12 PPR repeat family
PPR_2 PF13041 149 - 198 5e-08 PPR repeat family
PPR_1 PF12854 182 - 211 1.7e-07 PPR repeat
PPR_2 PF13041 188 - 232 1.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013547)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G25210
fragaria_vesca FvH4_3g25580
malus_domestica MD05G1166500.v1.1 MD10G1156500.v1.1
prunus_persica Prupe.8G194300_v2.0.a1
pyrus_communis pycom05g15510 pycom10g13470
rosa_chinensis RchiOBHm_Chr5g0046411
rosa_laevigata RLG00000034408
rosa_multiflora Rmu_sc0003878.1_g000050
rosa_roxburghii Rroxscaffold_1G00034990 Rroxscaffold_7G00197530
rosa_rugosa Rorug05G0227800
rosa_samantha Rh5AG309000 Rh5BG317200 Rh5CG343100 Rh5DG327400
rosa_wichuraiana Rw0G021510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 75
Acc36I ACCTGC 2 cut(s) 277, 295
AccB1I GGYRCC 1 cut(s) 442
AccII CGCG 1 cut(s) 315
AciI CCGC 4 cut(s) 36, 196, 225, 637
AfaI GTAC 3 cut(s) 182, 329, 457
AfiI CCNNNNNNNGG 1 cut(s) 492
AflIII ACRYGT 1 cut(s) 458
AgsI TTSAA 3 cut(s) 55, 88, 253
AluBI AGCT 2 cut(s) 46, 398
AluI AGCT 2 cut(s) 46, 398
Alw26I GTCTC 2 cut(s) 485, 552
ApeKI GCWGC 2 cut(s) 46, 130
AsuC2I CCSGG 1 cut(s) 593
AsuHPI GGTGA 1 cut(s) 475
BanI GGYRCC 1 cut(s) 442
BbvI GCAGC 2 cut(s) 33, 117
BccI CCATC 1 cut(s) 590
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciVI GTATCC 1 cut(s) 232
BclI TGATCA 1 cut(s) 258
BcnI CCSGG 1 cut(s) 593
BcoDI GTCTC 2 cut(s) 485, 552
BfaI CTAG 2 cut(s) 447, 626
BfuAI ACCTGC 2 cut(s) 277, 295
BfuI GTATCC 1 cut(s) 232
BisI GCNGC 2 cut(s) 47, 131
BlsI GCNGC 2 cut(s) 48, 132
Bme1390I CCNGG 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 444
BmrFI CCNGG 1 cut(s) 593
BmsI GCATC 3 cut(s) 286, 496, 637
BplI GAGNNNNNCTC 2 cut(s) 382, 414
Bpu10I CCTNAGC 2 cut(s) 146, 399
BpuMI CCSGG 1 cut(s) 593
BsaAI YACGTR 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 486
BsaWI WCCGGW 1 cut(s) 235
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse3DI GCAATG 3 cut(s) 125, 306, 363
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 3 cut(s) 338, 601, 652
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMI GCAATG 3 cut(s) 125, 306, 363
BseMII CTCAG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 33, 117
Bsh1236I CGCG 1 cut(s) 315
BshNI GGYRCC 1 cut(s) 442
BsiSI CCGG 2 cut(s) 236, 592
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 2 cut(s) 485, 552
BsmFI GGGAC 1 cut(s) 504
Bsp143I GATC 2 cut(s) 258, 619
Bsp19I CCATGG 1 cut(s) 486
Bsp68I TCGCGA 1 cut(s) 315
BspACI CCGC 4 cut(s) 36, 196, 225, 637
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 444
BspMI ACCTGC 2 cut(s) 277, 295
BspT107I GGYRCC 1 cut(s) 442
BsrDI GCAATG 3 cut(s) 125, 306, 363
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 2 cut(s) 258, 619
BssT1I CCWWGG 1 cut(s) 486
BstBAI YACGTR 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 189, 223
BstDEI CTNAG 2 cut(s) 146, 399
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 3 cut(s) 338, 601, 652
BstFNI CGCG 1 cut(s) 315
BstKTI GATC 2 cut(s) 261, 622
BstMAI GTCTC 2 cut(s) 485, 552
BstMBI GATC 2 cut(s) 258, 619
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 591
BstUI CGCG 1 cut(s) 315
BstV1I GCAGC 2 cut(s) 33, 117
BsuI GTATCC 1 cut(s) 232
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 3 cut(s) 338, 601, 652
BtuMI TCGCGA 1 cut(s) 315
BveI ACCTGC 2 cut(s) 277, 295
Cac8I GCNNGC 2 cut(s) 189, 223
Csp6I GTAC 3 cut(s) 181, 328, 456
CviAII CATG 3 cut(s) 19, 167, 487
CviJI RGCY 4 cut(s) 46, 191, 340, 398
CviKI_1 RGCY 4 cut(s) 46, 191, 340, 398
CviQI GTAC 3 cut(s) 181, 328, 456
DdeI CTNAG 2 cut(s) 146, 399
DpnI GATC 2 cut(s) 260, 621
DpnII GATC 2 cut(s) 258, 619
Eco130I CCWWGG 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 486
EcoT22I ATGCAT 2 cut(s) 127, 168
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 3 cut(s) 22, 170, 490
FaqI GGGAC 1 cut(s) 504
FatI CATG 3 cut(s) 18, 166, 486
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 47, 131
FokI GGATG 3 cut(s) 345, 608, 659
Fsp4HI GCNGC 2 cut(s) 47, 131
FspBI CTAG 2 cut(s) 447, 626
GluI GCNGC 2 cut(s) 47, 131
HapII CCGG 2 cut(s) 236, 592
Hin1II CATG 3 cut(s) 22, 170, 490
HincII GTYRAC 1 cut(s) 561
HindII GTYRAC 1 cut(s) 561
HinfI GANTC 1 cut(s) 109
HpaI GTTAAC 1 cut(s) 561
HpaII CCGG 2 cut(s) 236, 592
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 2 cut(s) 181, 561
Hpy188III TCNNGA 3 cut(s) 113, 314, 551
Hpy8I GTNNAC 2 cut(s) 181, 561
HpyAV CCTTC 4 cut(s) 211, 379, 575, 637
HpyCH4IV ACGT 3 cut(s) 242, 458, 572
HpyCH4V TGCA 8 cut(s) 102, 125, 166, 286, 299, 304, 477, 697
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpyF3I CTNAG 2 cut(s) 146, 399
HpySE526I ACGT 3 cut(s) 242, 458, 572
Hsp92II CATG 3 cut(s) 22, 170, 490
Ksp22I TGATCA 1 cut(s) 258
KspAI GTTAAC 1 cut(s) 561
Kzo9I GATC 2 cut(s) 258, 619
LpnPI CCDG 8 cut(s) 98, 249, 272, 290, 386, 564, 605, 636
Lsp1109I GCAGC 2 cut(s) 33, 117
LweI GCATC 3 cut(s) 286, 496, 637
MaeI CTAG 2 cut(s) 447, 626
MaeII ACGT 3 cut(s) 242, 458, 572
MaeIII GTNAC 1 cut(s) 359
MalI GATC 2 cut(s) 260, 621
MboI GATC 2 cut(s) 258, 619
MluCI AATT 3 cut(s) 13, 88, 97
MnlI CCTC 8 cut(s) 144, 384, 422, 508, 604, 622, 633, 703
Mph1103I ATGCAT 2 cut(s) 127, 168
MseI TTAA 3 cut(s) 156, 560, 686
MspI CCGG 2 cut(s) 236, 592
MspR9I CCNGG 1 cut(s) 593
MvnI CGCG 1 cut(s) 315
MwoI GCNNNNNNNGC 1 cut(s) 193
NciI CCSGG 1 cut(s) 593
NcoI CCATGG 1 cut(s) 486
NdeII GATC 2 cut(s) 258, 619
NlaIII CATG 3 cut(s) 22, 170, 490
NlaIV GGNNCC 1 cut(s) 444
NruI TCGCGA 1 cut(s) 315
NsiI ATGCAT 2 cut(s) 127, 168
NspI RCATGY 1 cut(s) 170
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 2 cut(s) 48, 132
Ppu21I YACGTR 1 cut(s) 459
PsiI TTATAA 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 444
RruI TCGCGA 1 cut(s) 315
RsaI GTAC 3 cut(s) 182, 329, 457
RsaNI GTAC 3 cut(s) 181, 328, 456
SaqAI TTAA 3 cut(s) 156, 560, 686
SatI GCNGC 2 cut(s) 47, 131
Sau3AI GATC 2 cut(s) 258, 619
ScrFI CCNGG 1 cut(s) 593
SfaNI GCATC 3 cut(s) 286, 496, 637
Sse9I AATT 3 cut(s) 13, 88, 97
SsiI CCGC 4 cut(s) 36, 196, 225, 637
SspMI CTAG 2 cut(s) 447, 626
StyD4I CCNGG 1 cut(s) 591
StyI CCWWGG 1 cut(s) 486
TaiI ACGT 3 cut(s) 245, 461, 575
TaqI TCGA 1 cut(s) 609
TasI AATT 3 cut(s) 13, 88, 97
TatI WGTACW 1 cut(s) 180
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 3 cut(s) 156, 560, 686
Tru9I TTAA 3 cut(s) 156, 560, 686
TseI GCWGC 2 cut(s) 46, 130
TspDTI ATGAA 4 cut(s) 230, 479, 674, 678
TspGWI ACGGA 2 cut(s) 345, 508
XceI RCATGY 1 cut(s) 170
XspI CTAG 2 cut(s) 447, 626
Zsp2I ATGCAT 2 cut(s) 127, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.