Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007043

Physical Location & Seq

Genomic Coordinates Reverse (-)

9271515 .. 9273751

Gene Structure 2237 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G25530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 870 bp

ATGGAAGTAGGGTTTCTGGGTTTGGGAATCATGGGAAAAGCCATGTCAATGAATCTATTGAAGAATGGATTCAAAGTCACTGTATGGAACAGAACACTCTCCAAGTGTGATGAGCTTGTGGAGCATGGTGCATCAGTATGTGAGAGTCCAGCTGAAGTAATCAAGAAATGCAAATACACTATTGCTATGCTCTCTGATCCTTGTGCTGCTCTTTCGGTTGTTTTCGATAAAGGCGGTGTTTTGGAGCAGATATGTGAAGGAAAAGGTTATATCGATATGTCGACTGTTGATGCAGAGACTTCTTTGAAGATCAATGAGGCAATCACCGGGAAGGGTGGTCGGTTCGTAGAAGGTCCGGTTTCAGGTAGCAAAAAGCCAGCTGAAGATGGCCAACTCATTATCCTTGCTGCTGGTGACAAGGCACTCTTTGAGGAATCAATCCCAGCTTTTGATGTCTTGGGGAAGAGATCGTTTTACTTGGGACAAGTTGGAAACGGAGCTAAAATGAAGCTAATAGTGAACATGATAATGGGAAGCATGATGAATGCATTCTCTGAGGGGCTTGTATTGGCTGACAAGAGTGGACTTAGCTCTGACACTCTTTTGGATATTCTGGATCTGGGAGCAATGACTAACCCGATGTTCAAGGGGAAAGGACCTTCAATGAACAAGAGTAGTTACCCACCAGCATTTCCATTGAAACATCAGCAGAAAGACATGAGGCTAGCTCTTGCTCTTGGCGATGAAAACGCGGTTTCCATGCCTGTAGCCGCGGCTGCAAACGAGGCTTTTAAGAAGGCGAGAAGCTTGGGACTAGGAGATCTCGACTTCTCTGCTGTGATTGAAGCTGTGAAATTCTCCCGCGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003858 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0016491 GO:0016614 GO:0016616 GO:0035064 GO:0042393 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055114 GO:0140030 GO:0140034

KEGG Pathways

Metabolic & Signaling

ko00630 ko00650 ko01100 ko01120 ko01200 map00630 map00650 map01100 map01120 map01200

Pfam Domains

Protein Families

NAD_binding_11 NAD_binding_2

Protein Analysis

289

Amino Acids

30.69

Weight (kDa)

5.88

Isoelectric Point (pI)

25.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_binding_2	PF03446	2 - 161	1.2e-43	NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11	PF14833	164 - 283	2e-26	NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007043)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25530 AT3G25530 AT3G25530
fragaria_vesca	FvH4_3g21710 FvH4_3g21710 FvH4_3g21710
malus_domestica	MD14G1000800.v1.1
prunus_persica	Prupe.7G000400_v2.0.a1 Prupe.7G000400_v2.0.a1
pyrus_communis	pycom49g00040
rosa_chinensis	RchiOBHm_Chr3g0497571
rosa_laevigata	RLG00000022451
rosa_multiflora	Rmu_sc0000212.1_g000018
rosa_roxburghii	Rroxscaffold_6G00387920
rosa_rugosa	Rorug03G0294100 Rorug03G0294200 Rorug03G0294200 Rorug03G0294200 Rorug03G0294300 Rorug03G0294400
rosa_samantha	Rh3AG337600 Rh3BG376200 Rh3CG371900 Rh3DG372500
rosa_wichuraiana	Rw0G011450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	281
AccII	CGCG	3 cut(s)	752, 773, 864
AciI	CCGC	5 cut(s)	234, 752, 771, 773, 862
AclWI	GGATC	2 cut(s)	191, 624
AcoI	YGGCCR	1 cut(s)	388
AcsI	RAATTY	1 cut(s)	854
AcuI	CTGAAG	2 cut(s)	174, 402
AfiI	CCNNNNNNNGG	1 cut(s)	362
AgsI	TTSAA	7 cut(s)	61, 73, 307, 646, 663, 700, 845
Alw26I	GTCTC	1 cut(s)	290
AlwI	GGATC	2 cut(s)	191, 624
AoxI	GGCC	1 cut(s)	388
ApeKI	GCWGC	3 cut(s)	206, 407, 776
ApoI	RAATTY	1 cut(s)	854
Asp700I	GAANNNNTTC	2 cut(s)	68, 548
AspS9I	GGNCC	2 cut(s)	353, 656
AsuC2I	CCSGG	1 cut(s)	328
AsuHPI	GGTGA	2 cut(s)	316, 425
AsuNHI	GCTAGC	1 cut(s)	724
AvaII	GGWCC	2 cut(s)	353, 656
BalI	TGGCCA	1 cut(s)	390
BbvI	GCAGC	3 cut(s)	193, 394, 763
BccI	CCATC	1 cut(s)	380
BcgI	CGANNNNNNTGC	2 cut(s)	815, 849
BcnI	CCSGG	1 cut(s)	328
BcoDI	GTCTC	1 cut(s)	290
BfaI	CTAG	2 cut(s)	725, 815
BfmI	CTRYAG	1 cut(s)	765
BglII	AGATCT	1 cut(s)	820
BisI	GCNGC	5 cut(s)	207, 408, 771, 774, 777
BlsI	GCNGC	5 cut(s)	208, 409, 772, 775, 778
Bme1390I	CCNGG	1 cut(s)	328
Bme18I	GGWCC	2 cut(s)	353, 656
BmgT120I	GGNCC	2 cut(s)	353, 656
BmrFI	CCNGG	1 cut(s)	328
BmsI	GCATC	2 cut(s)	140, 280
BmtI	GCTAGC	1 cut(s)	728
BplI	GAGNNNNNCTC	2 cut(s)	712, 744
BpuMI	CCSGG	1 cut(s)	328
Bsa29I	ATCGAT	1 cut(s)	273
BsaJI	CCNNGG	1 cut(s)	771
BsaWI	WCCGGW	1 cut(s)	355
Bsc4I	CCNNNNNNNGG	1 cut(s)	362
Bse3DI	GCAATG	1 cut(s)	633
BseCI	ATCGAT	1 cut(s)	273
BseDI	CCNNGG	1 cut(s)	771
BseLI	CCNNNNNNNGG	1 cut(s)	362
BseMI	GCAATG	1 cut(s)	633
BseMII	CTCAG	1 cut(s)	546
BseXI	GCAGC	3 cut(s)	193, 394, 763
BseYI	CCCAGC	1 cut(s)	442
Bsh1236I	CGCG	3 cut(s)	752, 773, 864
BshFI	GGCC	1 cut(s)	390
BshVI	ATCGAT	1 cut(s)	273
BsiSI	CCGG	2 cut(s)	327, 356
BslFI	GGGAC	2 cut(s)	495, 825
BslI	CCNNNNNNNGG	1 cut(s)	362
BsmAI	GTCTC	1 cut(s)	290
BsmFI	GGGAC	2 cut(s)	495, 825
BsmI	GAATGC	2 cut(s)	548, 550
BsnI	GGCC	1 cut(s)	390
Bsp143I	GATC	5 cut(s)	196, 309, 467, 616, 820
BspACI	CCGC	5 cut(s)	234, 752, 771, 773, 862
BspANI	GGCC	1 cut(s)	390
BspCNI	CTCAG	1 cut(s)	547
BspDI	ATCGAT	1 cut(s)	273
BspFNI	CGCG	3 cut(s)	752, 773, 864
BspOI	GCTAGC	1 cut(s)	728
BspPI	GGATC	2 cut(s)	191, 624
BsrDI	GCAATG	1 cut(s)	633
BssECI	CCNNGG	1 cut(s)	771
BssMI	GATC	5 cut(s)	196, 309, 467, 616, 820
Bst4CI	ACNGT	2 cut(s)	82, 286
Bst6I	CTCTTC	1 cut(s)	458
BstC8I	GCNNGC	2 cut(s)	378, 726
BstDEI	CTNAG	2 cut(s)	555, 587
BstDSI	CCRYGG	1 cut(s)	771
BstFNI	CGCG	3 cut(s)	752, 773, 864
BstKTI	GATC	5 cut(s)	199, 312, 470, 619, 823
BstMAI	GTCTC	1 cut(s)	290
BstMBI	GATC	5 cut(s)	196, 309, 467, 616, 820
BstMWI	GCNNNNNNNGC	3 cut(s)	121, 776, 785
BstSCI	CCNGG	1 cut(s)	326
BstSFI	CTRYAG	1 cut(s)	765
BstUI	CGCG	3 cut(s)	752, 773, 864
BstV1I	GCAGC	3 cut(s)	193, 394, 763
BstX2I	RGATCY	2 cut(s)	616, 820
BstYI	RGATCY	2 cut(s)	616, 820
Bsu15I	ATCGAT	1 cut(s)	273
BsuRI	GGCC	1 cut(s)	390
BsuTUI	ATCGAT	1 cut(s)	273
BtgI	CCRYGG	1 cut(s)	771
BtgZI	GCGATG	1 cut(s)	756
BtsIMutI	CAGTG	1 cut(s)	78
Cac8I	GCNNGC	2 cut(s)	378, 726
Cfr13I	GGNCC	2 cut(s)	353, 656
Cfr42I	CCGCGG	1 cut(s)	774
ClaI	ATCGAT	1 cut(s)	273
CviAII	CATG	7 cut(s)	31, 43, 125, 523, 538, 718, 760
DdeI	CTNAG	2 cut(s)	555, 587
DpnI	GATC	5 cut(s)	198, 311, 469, 618, 822
DpnII	GATC	5 cut(s)	196, 309, 467, 616, 820
EaeI	YGGCCR	1 cut(s)	388
Eam1104I	CTCTTC	1 cut(s)	458
EarI	CTCTTC	1 cut(s)	458
Eco47I	GGWCC	2 cut(s)	353, 656
Eco57I	CTGAAG	2 cut(s)	174, 402
EcoO109I	RGGNCCY	1 cut(s)	656
EcoT22I	ATGCAT	1 cut(s)	550
FaeI	CATG	7 cut(s)	34, 46, 128, 526, 541, 721, 763
FalI	AAGNNNNNCTT	2 cut(s)	410, 442
FaqI	GGGAC	2 cut(s)	495, 825
FatI	CATG	7 cut(s)	30, 42, 124, 522, 537, 717, 759
FblI	GTMKAC	1 cut(s)	281
Fnu4HI	GCNGC	5 cut(s)	207, 408, 771, 774, 777
Fsp4HI	GCNGC	5 cut(s)	207, 408, 771, 774, 777
FspBI	CTAG	2 cut(s)	725, 815
GluI	GCNGC	5 cut(s)	207, 408, 771, 774, 777
GsaI	CCCAGC	1 cut(s)	446
HaeIII	GGCC	1 cut(s)	390
HapII	CCGG	2 cut(s)	327, 356
Hin1II	CATG	7 cut(s)	34, 46, 128, 526, 541, 721, 763
HincII	GTYRAC	1 cut(s)	282
HindII	GTYRAC	1 cut(s)	282
HindIII	AAGCTT	1 cut(s)	805
HinfI	GANTC	5 cut(s)	27, 52, 69, 145, 434
HpaII	CCGG	2 cut(s)	327, 356
HphI	GGTGA	2 cut(s)	316, 425
Hpy166II	GTNNAC	3 cut(s)	282, 520, 584
Hpy188I	TCNGA	3 cut(s)	196, 556, 595
Hpy188III	TCNNGA	3 cut(s)	163, 614, 824
Hpy8I	GTNNAC	3 cut(s)	282, 520, 584
HpyAV	CCTTC	5 cut(s)	251, 325, 344, 669, 790
HpyCH4III	ACNGT	2 cut(s)	82, 286
HpyCH4V	TGCA	5 cut(s)	131, 171, 293, 548, 779
HpyF10VI	GCNNNNNNNGC	3 cut(s)	121, 776, 785
HpyF3I	CTNAG	2 cut(s)	555, 587
Hsp92II	CATG	7 cut(s)	34, 46, 128, 526, 541, 721, 763
KspI	CCGCGG	1 cut(s)	774
Kzo9I	GATC	5 cut(s)	196, 309, 467, 616, 820
LmnI	GCTCC	4 cut(s)	121, 244, 497, 623
Lsp1109I	GCAGC	3 cut(s)	193, 394, 763
LweI	GCATC	2 cut(s)	140, 280
MaeI	CTAG	2 cut(s)	725, 815
MaeIII	GTNAC	3 cut(s)	76, 413, 677
MalI	GATC	5 cut(s)	198, 311, 469, 618, 822
MboI	GATC	5 cut(s)	196, 309, 467, 616, 820
MboII	GAAGA	4 cut(s)	73, 319, 395, 475
MflI	RGATCY	2 cut(s)	616, 820
MlsI	TGGCCA	1 cut(s)	390
MluCI	AATT	1 cut(s)	854
MluNI	TGGCCA	1 cut(s)	390
MlyI	GAGTC	1 cut(s)	154
MmeI	TCCRAC	1 cut(s)	469
MnlI	CCTC	5 cut(s)	310, 424, 550, 714, 778
Mox20I	TGGCCA	1 cut(s)	390
Mph1103I	ATGCAT	1 cut(s)	550
MroXI	GAANNNNTTC	2 cut(s)	68, 548
MscI	TGGCCA	1 cut(s)	390
MseI	TTAA	1 cut(s)	792
MslI	CAYNNNNRTG	3 cut(s)	47, 136, 527
Msp20I	TGGCCA	1 cut(s)	390
MspA1I	CMGCKG	3 cut(s)	152, 380, 773
MspI	CCGG	2 cut(s)	327, 356
MspR9I	CCNGG	1 cut(s)	328
Mva1269I	GAATGC	2 cut(s)	548, 550
MvnI	CGCG	3 cut(s)	752, 773, 864
MwoI	GCNNNNNNNGC	3 cut(s)	121, 776, 785
NciI	CCSGG	1 cut(s)	328
NdeII	GATC	5 cut(s)	196, 309, 467, 616, 820
NheI	GCTAGC	1 cut(s)	724
NlaIII	CATG	7 cut(s)	34, 46, 128, 526, 541, 721, 763
NmuCI	GTSAC	2 cut(s)	76, 413
NsiI	ATGCAT	1 cut(s)	550
PctI	GAATGC	2 cut(s)	548, 550
PdmI	GAANNNNTTC	2 cut(s)	68, 548
PfeI	GAWTC	4 cut(s)	27, 52, 69, 434
PkrI	GCNGC	5 cut(s)	208, 409, 772, 775, 778
PleI	GAGTC	1 cut(s)	153
PpsI	GAGTC	1 cut(s)	153
PpuMI	RGGWCCY	1 cut(s)	656
Psp5II	RGGWCCY	1 cut(s)	656
PspFI	CCCAGC	1 cut(s)	442
PspPI	GGNCC	2 cut(s)	353, 656
PspPPI	RGGWCCY	1 cut(s)	656
PsuI	RGATCY	2 cut(s)	616, 820
PvuII	CAGCTG	2 cut(s)	152, 380
RseI	CAYNNNNRTG	3 cut(s)	47, 136, 527
SacII	CCGCGG	1 cut(s)	774
SalI	GTCGAC	1 cut(s)	280
SaqAI	TTAA	1 cut(s)	792
SatI	GCNGC	5 cut(s)	207, 408, 771, 774, 777
Sau3AI	GATC	5 cut(s)	196, 309, 467, 616, 820
Sau96I	GGNCC	2 cut(s)	353, 656
SchI	GAGTC	1 cut(s)	154
ScrFI	CCNGG	1 cut(s)	328
SfaNI	GCATC	2 cut(s)	140, 280
SfcI	CTRYAG	1 cut(s)	765
Sfr303I	CCGCGG	1 cut(s)	774
SgrBI	CCGCGG	1 cut(s)	774
SinI	GGWCC	2 cut(s)	353, 656
SmiMI	CAYNNNNRTG	3 cut(s)	47, 136, 527
Sse9I	AATT	1 cut(s)	854
SsiI	CCGC	5 cut(s)	234, 752, 771, 773, 862
SspMI	CTAG	2 cut(s)	725, 815
StyD4I	CCNGG	1 cut(s)	326
TaaI	ACNGT	2 cut(s)	82, 286
TaqI	TCGA	4 cut(s)	225, 273, 281, 825
TasI	AATT	1 cut(s)	854
TauI	GCSGC	2 cut(s)	773, 776
TfiI	GAWTC	4 cut(s)	27, 52, 69, 434
Tru1I	TTAA	1 cut(s)	792
Tru9I	TTAA	1 cut(s)	792
TscAI	CASTG	1 cut(s)	85
TseFI	GTSAC	2 cut(s)	76, 413
TseI	GCWGC	3 cut(s)	206, 407, 776
Tsp45I	GTSAC	2 cut(s)	76, 413
TspDTI	ATGAA	5 cut(s)	65, 521, 557, 680, 759
TspGWI	ACGGA	1 cut(s)	510
TspRI	CASTG	1 cut(s)	85
VpaK11BI	GGWCC	2 cut(s)	353, 656
XapI	RAATTY	1 cut(s)	854
XmiI	GTMKAC	1 cut(s)	281
XmnI	GAANNNNTTC	2 cut(s)	68, 548
XspI	CTAG	2 cut(s)	725, 815
Zsp2I	ATGCAT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.