Rorug03G0294300

Glyoxylate succinic semialdehyde reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007043
Physical Location & Seq
Reverse (-)
30249618 .. 30250097
480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0294300.1

Sequence Viewer

Length: 480 bp
ATGCCTCTTCGTTCCTCTATTGAACTTGCATCTACATTGGAACATTGGGTGAGACCCAATTTTCCCTCTTTGAAATTAAATGTGGATGGGGCTGTTGATACTAATGCCGACATGAGAGGGATATGGGCTGTTTTACTGAATCACTGTGGAGATTTCTTGTTGGCTGTATCAAAGGGCTTAAGAGGTGATTTCAGTGCTAAGGCAGTAGAACTTTATGCGGCTTTTCTGGGTCTCCAAACTATTAGGCAAAGGGGATTCACTGTGGTCTCTCTTATATTGGAAATGGATGCACTTGCAGTTGTTCAAGACTTAAATGATCAAAAACCAAATCTGTCGGTTGAAGGAGCATTAGTGGAGGAGGTGAAAGCCCTATTCTACCTCTTCTCTTCAGTGTTTTACACATATAGCCCTAGGAAATGTAACCAAGCAGCGCACATTTTAGCTAGGAAAACGCTTTTGTTTCCTGCATTTCAAATTTAG

Protein Analysis

159

Amino Acids

17.66

Weight (kDa)

6.82

Isoelectric Point (pI)

32.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 27 - 150 1e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 218
AcsI RAATTY 1 cut(s) 474
AcuI CTGAAG 1 cut(s) 372
AflII CTTAAG 1 cut(s) 178
AgsI TTSAA 5 cut(s) 23, 73, 305, 341, 473
AluBI AGCT 1 cut(s) 443
AluI AGCT 1 cut(s) 443
Alw26I GTCTC 3 cut(s) 46, 236, 271
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 1 cut(s) 474
AspA2I CCTAGG 1 cut(s) 410
AspLEI GCGC 1 cut(s) 433
AsuHPI GGTGA 3 cut(s) 61, 197, 373
AvrII CCTAGG 1 cut(s) 410
BbvI GCAGC 1 cut(s) 440
BccI CCATC 1 cut(s) 80
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 3 cut(s) 46, 236, 271
BfaI CTAG 2 cut(s) 411, 444
BfrI CTTAAG 1 cut(s) 178
BisI GCNGC 2 cut(s) 219, 429
BlnI CCTAGG 1 cut(s) 410
BlsI GCNGC 2 cut(s) 220, 430
BmsI GCATC 2 cut(s) 38, 277
Bpu10I CCTNAGC 1 cut(s) 198
BsaI GGTCTC 3 cut(s) 46, 236, 271
BsaJI CCNNGG 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
BseDI CCNNGG 1 cut(s) 410
BseGI GGATG 2 cut(s) 91, 292
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 1 cut(s) 440
BsmAI GTCTC 3 cut(s) 46, 236, 271
Bso31I GGTCTC 3 cut(s) 46, 236, 271
Bsp143I GATC 1 cut(s) 316
BspACI CCGC 1 cut(s) 218
BspTI CTTAAG 1 cut(s) 178
BspTNI GGTCTC 3 cut(s) 46, 236, 271
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 1 cut(s) 316
BssT1I CCWWGG 1 cut(s) 410
Bst4CI ACNGT 2 cut(s) 146, 262
Bst6I CTCTTC 3 cut(s) 12, 386, 391
BstAFI CTTAAG 1 cut(s) 178
BstDEI CTNAG 1 cut(s) 198
BstF5I GGATG 2 cut(s) 91, 292
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 1 cut(s) 319
BstMAI GTCTC 3 cut(s) 46, 236, 271
BstMBI GATC 1 cut(s) 316
BstV1I GCAGC 1 cut(s) 440
BtsCI GGATG 2 cut(s) 91, 292
BtsIMutI CAGTG 4 cut(s) 142, 199, 258, 396
CfoI GCGC 1 cut(s) 433
CviAII CATG 1 cut(s) 112
CviJI RGCY 8 cut(s) 92, 128, 164, 177, 221, 368, 408, 443
CviKI_1 RGCY 8 cut(s) 92, 128, 164, 177, 221, 368, 408, 443
DdeI CTNAG 1 cut(s) 198
DpnI GATC 1 cut(s) 318
DpnII GATC 1 cut(s) 316
Eam1104I CTCTTC 3 cut(s) 12, 386, 391
EarI CTCTTC 3 cut(s) 12, 386, 391
Eco130I CCWWGG 1 cut(s) 410
Eco31I GGTCTC 3 cut(s) 46, 236, 271
Eco57I CTGAAG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 410
FaeI CATG 1 cut(s) 115
FaiI YATR 6 cut(s) 113, 124, 216, 275, 403, 405
FatI CATG 1 cut(s) 111
FbaI TGATCA 1 cut(s) 316
Fnu4HI GCNGC 2 cut(s) 219, 429
FokI GGATG 2 cut(s) 98, 299
Fsp4HI GCNGC 2 cut(s) 219, 429
FspBI CTAG 2 cut(s) 411, 444
GlaI GCGC 1 cut(s) 432
GluI GCNGC 2 cut(s) 219, 429
HhaI GCGC 1 cut(s) 433
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HinfI GANTC 2 cut(s) 139, 255
HphI GGTGA 3 cut(s) 61, 197, 373
Hpy188III TCNNGA 1 cut(s) 305
HpyAV CCTTC 1 cut(s) 335
HpyCH4III ACNGT 2 cut(s) 146, 262
HpyCH4V TGCA 4 cut(s) 29, 290, 296, 467
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 1 cut(s) 431
Ksp22I TGATCA 1 cut(s) 316
Kzo9I GATC 1 cut(s) 316
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 1 cut(s) 212
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 2 cut(s) 38, 277
MaeI CTAG 2 cut(s) 411, 444
MaeIII GTNAC 1 cut(s) 419
MalI GATC 1 cut(s) 318
MboI GATC 1 cut(s) 316
MboII GAAGA 2 cut(s) 373, 378
MluCI AATT 3 cut(s) 58, 74, 474
MnlI CCTC 8 cut(s) 15, 25, 76, 110, 176, 349, 352, 389
MseI TTAA 3 cut(s) 77, 179, 311
MspCI CTTAAG 1 cut(s) 178
NdeII GATC 1 cut(s) 316
NlaIII CATG 1 cut(s) 115
PfeI GAWTC 2 cut(s) 139, 255
PkrI GCNGC 2 cut(s) 220, 430
SaqAI TTAA 3 cut(s) 77, 179, 311
SatI GCNGC 2 cut(s) 219, 429
Sau3AI GATC 1 cut(s) 316
SetI ASST 4 cut(s) 187, 363, 381, 445
SfaNI GCATC 2 cut(s) 38, 277
SmlI CTYRAG 1 cut(s) 178
SmoI CTYRAG 1 cut(s) 178
Sse9I AATT 3 cut(s) 58, 74, 474
SsiI CCGC 1 cut(s) 218
SspMI CTAG 2 cut(s) 411, 444
StyI CCWWGG 1 cut(s) 410
TaaI ACNGT 2 cut(s) 146, 262
TasI AATT 3 cut(s) 58, 74, 474
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 2 cut(s) 139, 255
Tru1I TTAA 3 cut(s) 77, 179, 311
Tru9I TTAA 3 cut(s) 77, 179, 311
TscAI CASTG 4 cut(s) 149, 199, 265, 396
TseI GCWGC 1 cut(s) 428
TspRI CASTG 4 cut(s) 149, 199, 265, 396
Vha464I CTTAAG 1 cut(s) 178
XapI RAATTY 1 cut(s) 474
XmaJI CCTAGG 1 cut(s) 410
XspI CTAG 2 cut(s) 411, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.