pycom49g00040

Glyoxylate succinic semialdehyde reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_49
OG0007043
Physical Location & Seq
Forward (+)
70088 .. 71428
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom49g00040.1

Sequence Viewer

Length: 378 bp
ATGGAGGTCGGGTTTTTGGGGCTGGGAATAATGGGGAAAGCCATGTCTATGAATCTGCTCCGGCATGGCTTCAAGGTCACCGTTTGGAACAGGACTCTTTCTAAGAGTCATGAACTCGTGGAGCATGGTGCTTCTGTTGCAGAAACCCCTGCAGAAGTGGTTAGGAAGTGCAAGTATACCATTGCAATGTTATCTGATCCTTCAGCTGCTCTTTCGGTGGTGTTTGGAAAAGATGGCATTCTTGAGCAGATTTGTGCTGGAAAATCTTATGTTGACATGTCTACAGTTGATGCTGATACTTCTTCAAAGATCAATGAGATACAGGATCTTCCTGATTCAGAATGCTATTTTGGGCCTTTTCGTGTAGTGAATATGTAG

Protein Analysis

126

Amino Acids

13.62

Weight (kDa)

5.54

Isoelectric Point (pI)

25.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_2 PF03446 2 - 106 2.1e-29 NAD binding domain of 6-phosphogluconate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 176, 281
AclWI GGATC 2 cut(s) 191, 333
AcuI CTGAAG 1 cut(s) 186
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 73, 306
AjuI GAANNNNNNNTTGG 2 cut(s) 333, 365
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
AlwI GGATC 2 cut(s) 191, 333
AoxI GGCC 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 70
BauI CACGAG 1 cut(s) 116
BbvI GCAGC 1 cut(s) 193
BccI CCATC 1 cut(s) 227
BfmI CTRYAG 2 cut(s) 150, 282
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 353
BmsI GCATC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 263
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse3DI GCAATG 2 cut(s) 180, 192
BseMI GCAATG 2 cut(s) 180, 192
BseXI GCAGC 1 cut(s) 193
BseYI CCCAGC 1 cut(s) 22
BshFI GGCC 1 cut(s) 355
BsiSI CCGG 1 cut(s) 61
BsmI GAATGC 2 cut(s) 237, 347
BsnI GGCC 1 cut(s) 355
Bsp143I GATC 3 cut(s) 196, 309, 325
BspANI GGCC 1 cut(s) 355
BspHI TCATGA 1 cut(s) 109
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 2 cut(s) 191, 333
BsrDI GCAATG 2 cut(s) 180, 192
BssMI GATC 3 cut(s) 196, 309, 325
BssNAI GTATAC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 116
Bst1107I GTATAC 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 82, 286
BstDEI CTNAG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 76
BstKTI GATC 3 cut(s) 199, 312, 328
BstMBI GATC 3 cut(s) 196, 309, 325
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 280
BstPI GGTNACC 1 cut(s) 76
BstSFI CTRYAG 2 cut(s) 150, 282
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BstZ17I GTATAC 1 cut(s) 177
BsuRI GGCC 1 cut(s) 355
CciI TCATGA 1 cut(s) 109
Cfr13I GGNCC 1 cut(s) 353
CviAII CATG 5 cut(s) 43, 65, 110, 125, 277
CviJI RGCY 5 cut(s) 22, 41, 69, 206, 355
CviKI_1 RGCY 5 cut(s) 22, 41, 69, 206, 355
DdeI CTNAG 1 cut(s) 102
DpnI GATC 3 cut(s) 198, 311, 327
DpnII GATC 3 cut(s) 196, 309, 325
Eco57I CTGAAG 1 cut(s) 186
Eco91I GGTNACC 1 cut(s) 76
EcoO65I GGTNACC 1 cut(s) 76
FaeI CATG 5 cut(s) 46, 68, 113, 128, 280
FaiI YATR 9 cut(s) 44, 50, 66, 111, 126, 177, 270, 278, 374
FatI CATG 5 cut(s) 42, 64, 109, 124, 276
FblI GTMKAC 2 cut(s) 176, 281
Fnu4HI GCNGC 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 207
GluI GCNGC 1 cut(s) 207
GsaI CCCAGC 1 cut(s) 26
HaeIII GGCC 1 cut(s) 355
HapII CCGG 1 cut(s) 61
Hin1II CATG 5 cut(s) 46, 68, 113, 128, 280
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 4 cut(s) 52, 94, 106, 335
HpaII CCGG 1 cut(s) 61
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 3 cut(s) 177, 274, 282
Hpy188I TCNGA 2 cut(s) 196, 340
Hpy188III TCNNGA 3 cut(s) 110, 242, 332
Hpy8I GTNNAC 3 cut(s) 177, 274, 282
HpyAV CCTTC 1 cut(s) 210
HpyCH4III ACNGT 2 cut(s) 82, 286
HpyCH4V TGCA 4 cut(s) 140, 152, 171, 185
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 1 cut(s) 102
Hsp92II CATG 5 cut(s) 46, 68, 113, 128, 280
Kzo9I GATC 3 cut(s) 196, 309, 325
LmnI GCTCC 2 cut(s) 63, 121
LpnPI CCDG 7 cut(s) 8, 74, 76, 162, 243, 308, 345
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 1 cut(s) 280
MaeIII GTNAC 1 cut(s) 76
MalI GATC 3 cut(s) 198, 311, 327
MboI GATC 3 cut(s) 196, 309, 325
MboII GAAGA 2 cut(s) 294, 320
MflI RGATCY 1 cut(s) 325
MlyI GAGTC 2 cut(s) 88, 115
MslI CAYNNNNRTG 2 cut(s) 47, 185
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 1 cut(s) 61
Mva1269I GAATGC 2 cut(s) 237, 347
MwoI GCNNNNNNNGC 1 cut(s) 137
NdeII GATC 3 cut(s) 196, 309, 325
NlaIII CATG 5 cut(s) 46, 68, 113, 128, 280
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 280
PagI TCATGA 1 cut(s) 109
PciI ACATGT 1 cut(s) 276
PctI GAATGC 2 cut(s) 237, 347
PfeI GAWTC 2 cut(s) 52, 335
PkrI GCNGC 1 cut(s) 208
PleI GAGTC 2 cut(s) 88, 114
PpsI GAGTC 2 cut(s) 88, 114
PscI ACATGT 1 cut(s) 276
PspEI GGTNACC 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 353
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 206
RseI CAYNNNNRTG 2 cut(s) 47, 185
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 3 cut(s) 196, 309, 325
Sau96I GGNCC 1 cut(s) 353
SchI GAGTC 2 cut(s) 88, 115
SetI ASST 3 cut(s) 9, 78, 208
SfaNI GCATC 1 cut(s) 280
SfcI CTRYAG 2 cut(s) 150, 282
SmiMI CAYNNNNRTG 2 cut(s) 47, 185
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
TaaI ACNGT 2 cut(s) 82, 286
TfiI GAWTC 2 cut(s) 52, 335
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 65, 126
XceI RCATGY 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 176, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.